Wide coverage of the tetraploid cotton genome using newly developed microsatellite markers.

Publication Overview
TitleWide coverage of the tetraploid cotton genome using newly developed microsatellite markers.
AuthorsNguyen TB; Giband M; Brottier P; Risterucci AM; Lacape JM
TypeJournal Article
Journal NameTheoretical and Applied Genetics
Volume109
Issue(1)
Year2004
Page(s)167 175
CitationNguyen TB, Giband M, Brottier P, Risterucci AM, Lacape JM. Wide coverage of the tetraploid cotton genome using newly developed microsatellite markers. Theoretical and applied genetics. 2004 Jun; 109(1):167-75
Publication CodeTAG-109-167

Abstract

Microsatellite (simple-sequence repeat (SSR)) markers were developed and positioned on the genetic map of tetraploid cotton. Three hundred and ninety-two unique microsatellite sequences, all but two containing a (CA/GT) repeat, were isolated, and the deduced primers were used to screen for polymorphism between the Gossypium hirsutum and G. barbadense parents of the mapping population analyzed in our laboratory. The observed rate of polymorphism was 56\\%. The 204 polymorphic SSRs revealed 261 segregating bands, which ultimately gave rise to 233 mapped loci. The updated status of our genetic map is now of 1,160 loci and 5,519 cM, with an average distance between two loci of 4.8 cM. The presence of a total of 466 microsatellite loci, with an average distance of 12 cM between two SSR loci, now provides wide coverage of the genome of tetraploid cotton and thus represents a powerful means for the production of a consensus map and for the effective tracking of QTLs.
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Featuremaps
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Map Name
Guazuncho-2 x VH8-4602, BC1 (2005)
Properties
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Property NameValue
Publication ModelPrint-Electronic
ISSN0040-5752
Publication Date2004 Jun
Journal AbbreviationTheor. Appl. Genet.
Language Abbreng
Publication TypeComparative Study
Publication TypeJournal Article
Journal CountryGermany
eISSN1432-2242
Journal AliasTheoretical and Applied Genetics. Theoretische und angewandte Genetik
Journal CodeTAG
LanguageEnglish
pISSN0040-5752
Publication CodeTAG-109-167
Published LocationGermany
Keywordsmicrosatellite; repetitive DNA; simple sequence repeats; SSR; Gossypium hirsutum; Gossypium barbadense; interspecific hybridization; microsatellite repeats; genetic markers; genetic polymorphism; loci; chromosome mapping; nucleotide sequences; alleles; gene segregation; genetic recombination; segregation distortion; DNA Primers; Gene Library; *Genome, Plant; Gossypium/*genetics; Hybridization, Genetic; Microsatellite Repeats/*genetics; Polymorphism, Genetic; Polyploidy; Sequence Analysis, DNA; x gossypium-barbadense; genetic-linkage maps; ssr markers; hirsutum l; l.; population; plants