Heat stress treated cotton (G.hirsutum L.) library

Library NameHeat stress treated cotton (G.hirsutum L.) library
Unique NameLIBEST_027679
OrganismGossypium hirsutum (Upland Cotton)
SNP Chip Base
Array NameHeat stress treated cotton (G.hirsutum L.) library
OrganismGossypium hirsutum (Upland Cotton)
Property NameValue
Vector typeBAC; plasmid
VectorPCR-TRAP (GenHunter)
Cloning site1: HindIII; 2: HindIII
Tissue typeleaf
Developmental stage77 day old plants with 4-5 squares (squaring stage)
HostE.coli DH5
CommentsDifferential display technique was used to compare expression patterns of heat treated and untreated cotton plants. Differentially expressed bands were excised from polyacrilamide gels and cDNAs were eluted. Extracted cDNAs were reamplified by PCR. PCR products were cloned to PCR-TRAP vector. Two direction sequencing was done using Lgh Primer (CGACAACACCGATAATC)and Rgh Primer (GACGCGAACGAAGCAAC).
Cross References
External references for this cDNA
NCBI BioSampleLIBEST_027679