|
|
Publication Overview
| Title | A simple method for isolation of megabase DNA from cotton. |
|---|
| Authors | Zhao XP; Zhang HB; Wing RA; Paterson AH |
|---|
| Type | Journal Article |
|---|
| Journal Name | Plant Molecular Biology Reporter - ISPMB |
|---|
| Volume | 12 |
|---|
| Year | 1994 |
|---|
| Page(s) | 110 115 |
|---|
| Citation | Zhao X, Zhang H, Wing R, Paterson A. A simple method for isolation of megabase DNA from cotton. Plant molecular biology reporter. 1994 June; 12(2):110-115. |
|---|
| Publication Code | ISP-12-110 |
|---|
Abstract
A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning.
Properties
Additional
details for this publication include:
| Property Name | Value |
|---|
| Journal Abbreviation | Plant mol. biol. report. |
| Language Abbr | eng |
| Publication Date | 1994 June |
| Publication Type | Journal Article |
| eISSN | 1572-9818 |
| Journal Alias | Plant Molecular Biology Reporter |
| Journal Code | ISP |
| Language | English |
| pISSN | 0735-9640 |
| Publication Code | ISP-12-110 |
| Published Location | Germany |
| URL | https://link.springer.com/article/10.1007/BF02668372 |
| Keywords | Gossypium hirsutum, molecular weight, genomics, isolation, DNA, genome analysis Gossypium high molecular weight DNA physical mapping pulsed-field gel electrophoresis |
|
