• Masonbrink RE, Gallagher JP, Jareczek JJ, Renny-Byfield S, Grover CE, Gong L, Wendel JF. CenH3 evolution in diploids and polyploids of three angiosperm genera. BMC Plant Biol. 2014 Dec 30;14:383. doi: 10.1186/s12870-014-0383-3.
BACKGROUND: Centromeric DNA sequences alone are neither necessary nor sufficient for centromere specification. The centromere specific histone, CenH3, evolves rapidly in many species, perhaps as a coevolutionary response to rapidly evolving centromeric DNA. To gain insight into CenH3 evolution, we characterized patterns of nucleotide and protein diversity among diploids and allopolyploids within three diverse angiosperm genera, Brassica, Oryza, ... [more]

Cotton Cluster File Request

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Dick Fabro
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Roberto Ricketts
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Ronda Rickman
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Viarp 54
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Trello https://trello.com/c/jPFD76SW/1-nhacai247 & Ronda Consulting
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Molly Pringle
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Rudolph Schnaars
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Corso Garibaldi 75
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Wallace Chiu
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About the assembly

DNA was extracted from leaves D1-35. Trimmed data were independently assembled for each species via ABySS v2.0.1 (Simpson et al. 2009), using every 5th kmer value from 40 through 100. A single assembly with the highest E-size (Salzberg et al. 2012) was selected for each species and subsequently annotated with MAKER v2.31.6 (Holt and Yandell 2011) using evidence from: 1) the NCBI G. raimondii EST database (Udall et al. 2006), 2) G. raimondii reference genome predicted proteins, as hosted by CottonGen.org (Paterson et al. 2012), and 3) three ab initio gene ... [more]

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