g.barbadense_cottongen_reftransV1_0098060, g.barbadense_cottongen_reftransV1_0098060 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy Swiss-Prot
Match: BRCA1_ARATH (Protein BREAST CANCER SUSCEPTIBILITY 1 homolog OS=Arabidopsis thaliana GN=BRCA1 PE=1 SV=1)

HSP 1 Score: 445.277 bits (1144), Expect = 2.432e-136
Identity = 226/416 (54.33%), Postives = 286/416 (68.75%), Query Frame = 2

Query: 1964 SIVKKLPLANGVALHKCE-TITIKIQCAFCLSMEETEASGEMVHYYEGSSVPLDYNGGSKVIHSHKNCTEWAPNVYFENDKAINLEAELSRSRKIKCSCCGLKGAALGCYEKSCRKTFHVPCAKLVSQCRWDVDNFVILCPLHASSKLPNEKSESQGIRKRRNLRGQLLIHHNEIPINDEVSVQKKWNTCESG--NKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFIIYSVELPVKCDPSKKHMILSRRQLNAEALASSTGAKAVSNSWVLSSISACKL 3202
            S + K        L +C  ++T K  CAFC   E+TEASGEM HYY G  V  D+NGGSKVIH HKNC EWAPNVYF +   +NL+ EL+RSR+I CSCCGLKGAALGCY KSC+ +FHV CAKL+ +CRWD   FV+LCPL AS KLP E++ S+  + +R  +  L  H     ++ + ++++       G   KLVL CSGLTVEEK +++EF +L+GVT+ K WDS +TH+IAS +ENGACKRTLK +M ILEGKWIL I W+KAC+K  K V E  YEIT+DVHGIR+GP LGR R   K+PKLF G KFYIMGDFE +YKG LQD+++AAGGTIL R+P+S D              +++SVE      PSKK  +  RR  +AEALA S  A+A S+SWVL SI+ C++
Sbjct:  538 STINKSSSGGSAHLRRCNGSLTKKFTCAFCQCSEDTEASGEMTHYYRGEPVSADFNGGSKVIHVHKNCAEWAPNVYFNDLTIVNLDVELTRSRRISCSCCGLKGAALGCYNKSCKNSFHVTCAKLIPECRWDNVKFVMLCPLDASIKLPCEEANSKDRKCKRTPKEPL--HSQPKQVSGKANIRELHIKQFHGFSKKLVLSCSGLTVEEKTVIAEFAELSGVTISKNWDSTVTHVIASINENGACKRTLKFMMAILEGKWILTIDWIKACMKNTKYVSEEPYEITMDVHGIREGPYLGRQRALKKKPKLFTGLKFYIMGDFELAYKGYLQDLIVAAGGTILRRRPVSSDDN-------EASTIVVFSVE------PSKKKTLTQRRS-DAEALAKSARARAASSSWVLDSIAGCQI 937          

HSP 2 Score: 187.193 bits (474), Expect = 2.667e-61
Identity = 115/257 (44.75%), Postives = 159/257 (61.87%), Query Frame = 2

Query:  323 SVCILKSMKSGSDCPVCKVPCRRREVRPAPHMDNLVSIYKSMEVASGFNIFVTQDAPLDTSADSGKRVKN-DLNCCERMDNQGANKGRGSREAKSTETSD-------PISVKPSFPTNKRVQVPQYPLSETSMLPEKNGGGLIEVTEDESKMSSDILKEN-NLGEKGEQILSPFFWLR-EEDVERPSQLSDGDQCLYITPPGLPSFSDMKDSDDEGFSKEEVHGNRDHLNLFDSEMFEWTQGACSPELLSSPDKMQV 1063
            + CI+KSMK  + CPVCK+P  RRE+R APHMD+LVSIYK+ME ASG  +FV+Q+ P  + +D  K+V++  +      + QG+ KGR S+  +  +T +       PI +KPS  T KRVQ+ Q  LS  S+          E  +D ++ +   L E+ +L ++G   LSPFFWLR E+D E  SQ ++ DQ L  TP  +PSFSD+ DSD E  SKE+     +  ++FDSEMFEWTQ  CSPE+L SP K +V
Sbjct:   37 NACIVKSMKMDATCPVCKIPYHRREIRGAPHMDSLVSIYKNMEDASGIKLFVSQNNP--SPSDKEKQVRDASVEKASDKNRQGSRKGRASKRNEYGKTKEIDVDAPGPIVMKPSSQTKKRVQLLQN-LSAESLTKPTESVETAEKPKDYTENTVIRLDEHPSLNKEGN--LSPFFWLRDEDDGENSSQRTESDQLLGTTPVNVPSFSDLMDSDHESPSKEDEQQKPNPGDMFDSEMFEWTQRPCSPEILPSPVKAKV 288          

HSP 3 Score: 73.1738 bits (178), Expect = 2.667e-61
Identity = 31/36 (86.11%), Postives = 33/36 (91.67%), Query Frame = 3

Query:  102 MGDPSHLEKMGRELKCPICLSLLNSAVSLTCNHVFC 209
            M D SHLE+MGRELKCPICLSL NSAVSL+CNHVFC
Sbjct:    1 MADTSHLERMGRELKCPICLSLYNSAVSLSCNHVFC 36          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy Swiss-Prot
Match: BARD1_ARATH (BRCA1-associated RING domain protein 1 OS=Arabidopsis thaliana GN=BARD1 PE=1 SV=1)

HSP 1 Score: 353.599 bits (906), Expect = 6.375e-105
Identity = 177/394 (44.92%), Postives = 244/394 (61.93%), Query Frame = 2

Query: 2021 ITIKIQCAFCLSMEETEASGEMVHYYEGSSVPLDYNGGSKVIHSHKNCTEWAPNVYFENDKAINLEAELSRSRKIKCSCCGLKGAALGCYEKSCRKTFHVPCAKLVSQCRWDVDNFVILCPLHASSKLPNEKSESQGIRKRRNLRGQLLIHHNEIPINDEVSVQKKWNTCESGNKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFIIYSVELPVKCDPSKKHMILSRRQLNAEALASSTGAKAVSNSWVLSSISACKL 3202
            ITI   C FC S   +EA+GEM+HY  G  V  D    S VIH H  C EWAP VY+E D   NL+AEL+R  KIKC+ C LKGAALGC+ KSCR+++HVPCA+ +S+CRWD ++F++LCP H+S K PNEKS            G  +     +P  +   +     T     +LVLC S L+  +K++M         T+ + W+ ++TH+IASTDE GAC RTLK+LMGIL GKWI+N  W+KA +KA + VDE  +EI ID  G +DGP+  RLR +  +PKLF+G KFY  GDF   YK DLQ+++  AGGTIL+ +   G   +  +        ++Y+++ P  C   ++  I+ +R  +AEALAS TG++ V ++WVL SI+  KL
Sbjct:  331 ITI---CGFCQSARVSEATGEMLHYSRGRPVDGDDIFRSNVIHVHSACIEWAPQVYYEGDTVKNLKAELARGMKIKCTKCSLKGAALGCFVKSCRRSYHVPCAREISRCRWDYEDFLLLCPAHSSVKFPNEKS------------GHRVSRAEPLPKINPAELCSLEQTPAFTKELVLCGSALSKSDKKLMESLAVRFNATISRYWNPSVTHVIASTDEKGACTRTLKVLMGILNGKWIINAAWMKASLKASQPVDEEPFEIQIDTQGCQDGPKTARLRAETNKPKLFEGLKFYFFGDFYKGYKEDLQNLVKVAGGTILNTEDELGAESSNNVNDQRSSSIVVYNIDPPHGCALGEEVTIIWQRANDAEALASQTGSRLVGHTWVLESIAGYKL 709          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy Swiss-Prot
Match: BARD1_HUMAN (BRCA1-associated RING domain protein 1 OS=Homo sapiens GN=BARD1 PE=1 SV=2)

HSP 1 Score: 112.849 bits (281), Expect = 6.501e-24
Identity = 61/148 (41.22%), Postives = 85/148 (57.43%), Query Frame = 2

Query: 2546 LVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKP 2989
            LVL  SGL+ E+++++SE   +        +DS +TH++   D   A + TLK ++GIL G WIL  +WVKAC++      E KYEI        +GPR  RL  +   PKLFDGC FY+ G F+   K +L  ++ A GG IL RKP
Sbjct:  570 LVLIGSGLSSEQQKMLSELAVILKAKKYTEFDSTVTHVVVPGD---AVQSTLKCMLGILNGCWILKFEWVKACLRRKVCEQEEKYEIP-------EGPRRSRLNREQLLPKLFDGCYFYLWGTFKHHPKDNLIKLVTAGGGQILSRKP 707          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy Swiss-Prot
Match: BARD1_RAT (BRCA1-associated RING domain protein 1 OS=Rattus norvegicus GN=Bard1 PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 2.077e-23
Identity = 66/182 (36.26%), Postives = 101/182 (55.49%), Query Frame = 2

Query: 2447 NLRGQLLIHHNEIPIN-DEVSVQKKWNTCESGNKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKP 2989
            N+R  LL+       +  + SVQ      +SG  LVL  SGL+ ++++++S+ E +        +D+ +TH+I   +E    + TLK ++GIL G W+L   WVKAC+ + +   E KYE+         GP+  RL  +   PKLFDGC F++ G+F+   K DL  ++ AAGG IL RKP
Sbjct:  528 NIRSLLLLPEKTDSFSTSQCSVQVNTGQRKSG-PLVLIGSGLSSQQQKLLSKLETVLKAKKCAEFDNTVTHVIVPDEE---AQSTLKCMLGILNGCWVLKFDWVKACLDSQEREQEEKYEVP-------GGPQRSRLNREQLLPKLFDGCYFFLGGNFKHHPKEDLLKLIAAAGGRILSRKP 698          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy Swiss-Prot
Match: BARD1_MOUSE (BRCA1-associated RING domain protein 1 OS=Mus musculus GN=Bard1 PE=2 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 1.889e-22
Identity = 66/183 (36.07%), Postives = 100/183 (54.64%), Query Frame = 2

Query: 2447 NLRGQLLI-HHNEIPINDEVSVQKKWNTCESGN-KLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKP 2989
            N+R  LL+   NE     + S+    NT +  N  LV   SGL+ ++++++S+ E +        +DS +TH+I   +E    + TLK ++GIL G WIL   WVKAC+ +     E KYE+         GP+  RL  +   PKLFDGC F++ G+F+   + DL  ++ AAGG +L RKP
Sbjct:  526 NIRSLLLLPEENESFSTSQCSI---VNTGQRKNGPLVFIGSGLSSQQQKMLSKLETVLKAKKCMEFDSTVTHVIVPDEE---AQSTLKCMLGILSGCWILKFDWVKACLDSKVREQEEKYEVP-------GGPQRSRLNREQLLPKLFDGCYFFLGGNFKHHPRDDLLKLIAAAGGKVLSRKP 695          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy Swiss-Prot
Match: BRCA1_BOVIN (Breast cancer type 1 susceptibility protein homolog OS=Bos taurus GN=BRCA1 PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 3.509e-13
Identity = 50/163 (30.67%), Postives = 84/163 (51.53%), Query Frame = 2

Query: 2522 NTCESGNKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRD--GPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDP 3004
            +T  S  +L +  SGLT +E  ++ +F +   VT+        TH+I  TD    C+RTLK  +GI  GKW+++  WV   IK  K++DE  +E+  DV   R+  GP+  R   +++  K+F G +    G F       L+ ++   G +++ ++P S  P
Sbjct: 1635 STERSKKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKEGKMLDEHDFEVRGDVVNGRNHQGPKRAR---ESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVV-KEPSSFTP 1793          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy Swiss-Prot
Match: BRCA1_MOUSE (Breast cancer type 1 susceptibility protein homolog OS=Mus musculus GN=Brca1 PE=1 SV=3)

HSP 1 Score: 78.1814 bits (191), Expect = 4.244e-13
Identity = 50/161 (31.06%), Postives = 83/161 (51.55%), Query Frame = 2

Query: 2546 LVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRD--GPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKP-ISGDPGARLL 3019
            + +  SGLT +E   + +F +   +T+        TH+I  TD    C+RTLK  +GI  GKWI++  WV   I+  +L++  ++E+  DV   R+  GPR    R +  + KLF G + Y    F    K +L+ +L   G +++   P ++ D GA L+
Sbjct: 1593 ISMVVSGLTPKEVMTVQKFAEKYRLTLTDAITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVVRSIQERRLLNVHEFEVKGDVVTGRNHQGPR----RSRESREKLFKGLQVYCCEPFTNMPKDELERMLQLCGASVVKELPSLTHDTGAHLV 1749          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy Swiss-Prot
Match: BRCA1_RAT (Breast cancer type 1 susceptibility protein homolog OS=Rattus norvegicus GN=Brca1 PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.614e-12
Identity = 50/158 (31.65%), Postives = 79/158 (50.00%), Query Frame = 2

Query: 2546 LVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDV--HGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKP-ISGDPGA 3010
            + +  SGLT +E  I+ +F +   + +        TH+I  TD    C+RTLK  +GI  GKWI++  WV   I+  KL+   ++E+  DV       GPR    R +  Q KLF+G + Y    F    K +L+ +L   G +++   P ++ D GA
Sbjct: 1596 ISMVVSGLTPKEVMIVQKFAEKYRLALTDVITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVIKSIQERKLLSVHEFEVKGDVVTGSNHQGPR----RSRESQEKLFEGLQIYCCEPFTNMPKDELERMLQLCGASVVKELPLLTRDTGA 1749          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy Swiss-Prot
Match: BRCA1_PONPY (Breast cancer type 1 susceptibility protein homolog OS=Pongo pygmaeus GN=BRCA1 PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.237e-12
Identity = 48/171 (28.07%), Postives = 87/171 (50.88%), Query Frame = 2

Query: 2543 KLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRD--GPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFII 3049
            ++ +  SGLT EE  ++ +F +   +T+        TH++  TD    C+RTLK  +GI  GKW+++  WV   IK  K+++E  +E+  DV   R+  GP+  R   +++  K+F G +    G F       L+ ++   G +++  K +S    +  LG+   PI ++
Sbjct: 1649 RMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRAR---ESQDRKIFRGLEICCYGPFTNMPTDQLEWIVQLCGASVV--KELS----SFTLGTGVHPIVVV 1810          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy Swiss-Prot
Match: BRCA1_PANTR (Breast cancer type 1 susceptibility protein homolog OS=Pan troglodytes GN=BRCA1 PE=2 SV=2)

HSP 1 Score: 75.0998 bits (183), Expect = 3.530e-12
Identity = 48/171 (28.07%), Postives = 87/171 (50.88%), Query Frame = 2

Query: 2543 KLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRD--GPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFII 3049
            ++ +  SGLT EE  ++ +F +   +T+        TH++  TD    C+RTLK  +GI  GKW+++  WV   IK  K+++E  +E+  DV   R+  GP+  R   +++  K+F G +    G F       L+ ++   G +++  K +S    +  LG+   PI ++
Sbjct: 1649 RMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRAR---ESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVV--KELS----SFTLGTGVHPIVVV 1810          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy TrEMBL
Match: B9SYJ0_RICCO (Brca1 associated ring domain, putative OS=Ricinus communis GN=RCOM_1288560 PE=4 SV=1)

HSP 1 Score: 763.837 bits (1971), Expect = 0.000e+0
Identity = 482/996 (48.39%), Postives = 619/996 (62.15%), Query Frame = 2

Query:  329 CILKSMKSGSDCPVCKVPCRRREVRPAPHMDNLVSIYKSMEVASGFNIFVTQDAPLDTSADSGKRVKNDLNC--------CE-RMDNQGANKGRGSREA--KSTETSDPISVKPSFPTNKRVQVPQYPLSETSMLPEKNGGGLIEVTEDES-----KMSSDILKENNL-GEKGEQILSPFFWLR-EEDVERPSQLSDGDQCLYITPPGLPSFSDMKDSDDEGFSK----EEVHGNRDHLNLFDSEMFEWTQGACSPELLSSP--DKMQVAD--TYEPASQGA-STAEPCNNGTCKITGHDASEEMLPNIESLRIQCVSSNKIRSCKSKELGKKQRKTAQKNIADRTSSHISGDHVNLGKGSEDFNKKQKDDCSSSSYLAKTRKNCKVAGLDHHVTEPMASHFSAK--APKQEDTPLAAIVGMGKCGDENENLRMVKRCRKITAXXXXXXXXXXXXXXXXXXXXDTPKEVLTVCGQANEYMIQQKSSLPVPLAHENAAEQFR-KGLSKHTREVKL-APTLKSEKNSGC--KKKIKVYFSDDMKSWLPEDQQQQGGNANI----SLERVSEKVHGRTTVGNSESSIVK------------KLPLANGVALHKCETITIKIQCAFCLSMEETEASGEMVHYYEGSSVPLDYNGGSKVIHSHKNCTEWAPNVYFENDKAINLEAELSRSRKIKCSCCGLKGAALGCYEKSCRKTFHVPCAKLVSQCRWDVDNFVILCPLHASSKLPNEKSESQ-GIRKRRNLRGQLLIHHNEIPINDEVSVQKKWNTCESGNKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFIIYSVELPVKCDPSKKHMILSRRQLNAEALASSTGAKAVSN 3166
            CI+KSMKSGS+CPVCKVP +RREVR APHMDNLV+IYKSME ASGF IFVTQD P      S K ++ + NC        C+  ++NQ   K +GSR+    + E SDPI+ KPSFPT KRVQVPQ   S+T    EK     +E+  DE+     K SS    EN +  EKGE + SPFFWLR EED+E+ SQ + G Q L ITPP +P+FSD+KDSDDE  SK    EEV G  +  + FDSEMFEWTQ ACSPEL S+P   KM VAD  +  P++ G  ST     N      G  A + ML       ++     ++++C +  L +K ++T QK             H N G  S DFNK +K    + S  + T        L H    P++    +   + K+ D  L + VG  +C D++    + K C KI+  C+     + KK+KL  +  D                I++  ++  P   E +  +   K +S+  R+ +L +  L SE N     KK +KV+F    +  +  D Q+   N +     S+E V++ V  R T    ++ I+             KL  ANG AL  C+ ++ K +C+FCLS EE+EASGEMVHYY G  V   Y GG KVIHSH+NC EWAPNVYFE+D AINLEAEL+RSR+IKC CCGLKGAALGCYEKSCRK+FHV CAK++ QCRWD DNFV LCPLHASSKLPNE S +Q  IRK+   + Q     N++ + D VS    WN+  + + L+LCCSGLTVEE+EI+SEF++L+GVTV K WD ++TH+IASTD NGACKRTLKILMGILEGKWILNI+WVKAC+ AMK V E  +E+ +D+HGIRDGPRLGR+R+  KQPK+F+G +FY MGDF  SYKG +QD++ A GGTILHRKP+   PGA    SP+   FIIYS ELP KCD  KK MIL+RR+ +AEALAS T AKA+++
Sbjct:   39 CIVKSMKSGSNCPVCKVPYQRREVRAAPHMDNLVNIYKSMEAASGFQIFVTQDPP------STKLLEGNTNCRREDIQRICQDTVENQRMGKRKGSRKKFKSNLEFSDPIAAKPSFPTKKRVQVPQSLPSKTPTRNEK-----LEIKSDENIRDGFKNSSLAQNENPVPSEKGEPVFSPFFWLRDEEDIEKLSQHTVGSQFLDITPPHVPAFSDIKDSDDEYPSKLSPEEEVCGKSNDADFFDSEMFEWTQRACSPELYSTPFKHKMTVADEDSSIPSTLGPKSTNTQIVNDKSNRRGRTARKAMLKKCAKKDVE----QELKACFN--LKRKTKETMQKEA-----------HENKG-DSLDFNKNRKSRKKTVSGTSSTE-------LKHESVLPVSLRGESLNLSDKRLDADLPSSVGENQC-DKDFKRNITKICGKISTLCRRAHSLRSKKRKLESIIND----------------IEENDAVQNPEDEEESPSKIDGKNVSEVKRKCQLHSSALSSENNRKLRQKKNMKVFFHGISEDGILTDHQEGQSNTSTKETQSIEMVTD-VFTRQTQFIEKAQIIPGSRIQNNPVMLGKLDRANGAALQSCQALSRKTRCSFCLSSEESEASGEMVHYYNGRPVAASYGGGYKVIHSHRNCAEWAPNVYFEDDTAINLEAELTRSRRIKCCCCGLKGAALGCYEKSCRKSFHVTCAKMIPQCRWDTDNFVTLCPLHASSKLPNEDSLTQERIRKKCIPKRQQSNQCNQVDLKD-VSTCPSWNSSLTPDNLILCCSGLTVEEREIVSEFQRLSGVTVFKNWDLSVTHVIASTDVNGACKRTLKILMGILEGKWILNIEWVKACMNAMKPVQEDPFEVLVDIHGIRDGPRLGRIRILKKQPKIFEGLQFYFMGDFVASYKGYIQDLIFAGGGTILHRKPL---PGAEGASSPS--TFIIYSTELPDKCDIKKKDMILNRRKADAEALASETEAKALAD 974          

HSP 2 Score: 73.9442 bits (180), Expect = 5.943e-10
Identity = 33/36 (91.67%), Postives = 34/36 (94.44%), Query Frame = 3

Query:  102 MGDPSHLEKMGRELKCPICLSLLNSAVSLTCNHVFC 209
            MGDP HLEKMG ELKCPICLSLLNSAVSLTCNH+FC
Sbjct:    1 MGDPIHLEKMGTELKCPICLSLLNSAVSLTCNHIFC 36          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy TrEMBL
Match: M5WNS3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000720mg PE=4 SV=1)

HSP 1 Score: 743.421 bits (1918), Expect = 0.000e+0
Identity = 480/1005 (47.76%), Postives = 617/1005 (61.39%), Query Frame = 2

Query:  329 CILKSMKSGSDCPVCKVPCRRREVRPAPHMDNLVSIYKSMEVASGFNIFVTQDAPLDTSADSGKRVKND--------LNCCERMDNQGANKGRGSREAKST-ETSDPISVKPSFPTNKRVQVPQYPLSETSMLPEKNGGGLIEVTEDESKMSSDILKENN-LGEKGEQILSPFFWLREEDVERPSQLSDGDQCLYITPPGLPSFSDMKDSDDEGFSK----EEVHGNRDHL-NLFDSEMFEWTQGACSPELLSSPDKMQVADTYE-PASQGASTAEPCNNGTCKITGHDA-------SEEMLPNIESL-RIQCVSSNKIRSCKSKELGKKQRKTAQKNIADR---TSSHISGDHVNLG---KGSEDFNKKQ--KDDCSSSSYLAKTRKNCKVAGLDHHVTEPMASHFSAKAPKQED------TPLAAIVGMGKCGDENENLRMVKRCRKITAXXXXXXXXXXXXXXXXXXXXDTPKEVLTVCGQANEYMIQQKSSLPVPLAHENAAEQFRKGLSKHTREVKLA--------PTLKSEKNSGCKKKIKVYFSDDMKSWLPEDQQQQGGNANISLERVSEKVHGRTTVGNSESSIVKKLP-LANGVALHKCETITIKIQCAFCLSMEETEASGEMVHYYEGSSVPLDYNGGSKVIHSHKNCTEWAPNVYFENDKAINLEAELSRSRKIKCSCCGLKGAALGCYEKSCRKTFHVPCAKLVSQCRWDVDNFVILCPLHASSKLPNEKSESQGIRKRRNLRGQLLIHHNEIPINDEVSVQKKWNTCESGNKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFIIYSVELPVKCDPSKKHMILSRRQLNAEALASSTGAKAVSNSWVLSSISACKL 3202
            CI+KSMKSGS+CPVCK+P RRRE+RPAPHMDNLVSIYKSMEVASG NIFVTQ+AP   S+D   + ++D         +C +R + Q + +G+GSR+ KS  + S  +SVKPSFPT KRVQVPQ PLSET   PEK  G L++   + SK SS  LK    L E GE +LSPFFWLR++D E  SQ +DGDQ L  +PP +P+FSD+KDS+DE  ++     EVHG   ++ +LFDSEMFEWTQ  CSPEL  SP KMQV D+ +    Q     E   N   K+  H A       S +   N+  L  I    +       S EL K+ R+T  +NI+ R   TS    G  V++    KGS+ F ++   K++ SS + ++K  K    + +    T       S +A  Q +      T     +G  + G    + +  K C ++ A+ Q     + +KQK+  +     +EV  V  QAN+      S + +       A +F     K  R  KL          T+ S+ NS           DD    + E      GN N +  + +EK+ G   V  ++ S V+KLP L N + L +CE I  KIQCAFCLS EE+EASGE+VHYY G  V +D+NGG KVIH+H+ CTEW                    SR+IKCSCC +KGAALGCYE+SCRK+FHV CAKL+ +CRWD+DNFV+LCPLHASSKLPNE SESQ  R++ N R Q    H +  +  + ++      C S  KLVLCCS LT  E+E +S FE+L+G TVLK WDS++TH+IAS DENGAC+RTLK+LMGILEG+WIL+++W+ ACI+AMKLV+E  YEI +D++GIRDGPRLGRLRLQNKQPKLFDG KFY MGDF PSYKG LQD++IAAGGTILHRKP+     A    SP    FIIYS+E P +  PSK   IL RRQ +A+ALASS GA A SNSW+L+SI+ACKL
Sbjct:   39 CIVKSMKSGSNCPVCKIPYRRREIRPAPHMDNLVSIYKSMEVASGINIFVTQNAPSTKSSDGKLQAQDDGHDEQDIPKHCQDRDEKQKSLRGKGSRKTKSNLKNSGSLSVKPSFPTKKRVQVPQCPLSETPTRPEKTVGKLMD---ERSKHSSTTLKGRPVLNEVGEPMLSPFFWLRDKDEENLSQHTDGDQ-LTDSPPNVPTFSDIKDSEDEYSARLTPPGEVHGKSSNVADLFDSEMFEWTQRECSPELFQSPSKMQVPDSDDIDRVQEKELKEISQNK--KLDAHSAKNARSRNSRQSSGNMYFLPDIPPAGAKDNSQVVSNELNKQGRQT--RNISKRKCATSHTDPGADVSVNVNSKGSKVFYEEPVCKNNSSSLAKISKRSKKEHPSVVAMKPTSENVHVLSTEAETQNNGDDKGITESPTSLGKSEGGSAPPSKKAGKICNEVNAKSQMHCPVRSRKQKMVSMPNKMLEEVSEVQKQANKDTTTDLSLINLSTVDNKKASEFGNKSRKLQRGAKLCDRELKNKKATVSSDGNS----------KDDTFVRVGEGH----GNVNENGNQPTEKIEGNCDVP-TDRSPVQKLPSLTNNLVLQRCEAIPSKIQCAFCLSSEESEASGEIVHYYNGKPVAVDHNGGLKVIHAHRICTEW--------------------SRRIKCSCCEIKGAALGCYERSCRKSFHVTCAKLMPECRWDIDNFVMLCPLHASSKLPNESSESQARRRKSNPRKQSNAEHYKAAVKQDNTMPPDRKFCGSSKKLVLCCSSLTNAERESVSGFERLSGFTVLKNWDSSVTHVIASVDENGACRRTLKVLMGILEGRWILSMEWINACIEAMKLVNEEPYEINVDIYGIRDGPRLGRLRLQNKQPKLFDGFKFYFMGDFVPSYKGYLQDLVIAAGGTILHRKPVPEGQKAFSASSPKCQTFIIYSLEQPEQGHPSKG-TILDRRQSDAKALASSAGAMAASNSWILNSIAACKL 999          

HSP 2 Score: 71.2478 bits (173), Expect = 4.520e-9
Identity = 32/36 (88.89%), Postives = 33/36 (91.67%), Query Frame = 3

Query:  102 MGDPSHLEKMGRELKCPICLSLLNSAVSLTCNHVFC 209
            M  P+HLEKMGRELKCPICLSLLNSAVSL CNHVFC
Sbjct:    1 METPAHLEKMGRELKCPICLSLLNSAVSLNCNHVFC 36          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy TrEMBL
Match: A0A072V4U2_MEDTR (Breast cancer susceptibility-like protein, putative OS=Medicago truncatula GN=MTR_2g018880 PE=4 SV=1)

HSP 1 Score: 700.279 bits (1806), Expect = 0.000e+0
Identity = 432/1010 (42.77%), Postives = 582/1010 (57.62%), Query Frame = 2

Query:  329 CILKSMKSGSDCPVCKVPCRRREVRPAPHMDNLVSIYKSMEVASGFNIFVTQDAPLDTSADSGKRVKNDLNCCER----------MDNQGANKGRGSREAKSTETSD---------PIS--VKPSFPTNKRVQVPQYPLSETSMLPEKNGGGLIEVTEDESKMSSDILKENNLG-EKGEQILSPFFWLREE-DVERPSQLSDGDQCLYITPPGLPSFSDMKDSDDEGFSKE----EVHGNRDHLNLFDSEMFEWTQGACSPELLSSPDKMQVADTYE----------PASQGASTAEPCNNGTCKITGHDASEEMLPNIESLRIQCVSSNKIRSCKSKELGKKQRKTAQKNIADRTSS-HISGDHVNLGKG--SEDFNKKQKDDCSSSSYLAKTRKNCKVAGL--DHHVTEPMASHFSAKAPKQEDTPLAAIVGMGKCGD----------ENENLRMVKRCRKITAXXXXXXXXXXXXXXXXXXXXDTPKEVLTVCGQANEYMIQQKSSLPVPLAHENAAEQFRKGLSKHTREVKLAPTLKSEKNSGCKKKIKVYFSDDMKSWLPEDQQQQGGNANISLERVSEKVHGRTTVGNSESSIVKKLPLANGVALHKCETITIKIQCAFCLSMEETEASGEMVHYYEGSSVPLDYNGGSKVIHSHKNCTEWAPNVYFENDKAINLEAELSRSRKIKCSCCGLKGAALGCYEKSCRKTFHVPCAKLVSQCRWDVDNFVILCPLHASSKLPNEKSESQGIRKRRNLRGQLLIHHNEIPINDEVSVQKKWNTCESGNKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFIIYSVELPVKCDPSKKHMILSRRQLNAEALASSTGAKAVSNSWVLSSISACKL 3202
            CI KSMKS S CPVCK+P  RREVRPAPH+DNLV+IYK+ME ASG N+F TQ+     S D   +   D    E+           + +   + R  ++ K++  S          P S   KPSFP  KRVQVPQ  LSET +   K GG + +V ++ ++ +S     + +G EK +Q+  PFFWLREE D ER S  +D DQ +    P  PSFSD+KD+DDE   K+    EV  N+  ++LFDSE FEWTQ  CSPEL SSP K QV DT E             +  ++ + CN       G+  +E +LP + S   +  SS+ +     K   K+ RK  +K+  D     +   D +N+     S+   ++  D    +S L K+ K  K          T P+A   S             I+G+   GD          E++    ++   KI  + Q +   K +   +     D P+++ +V  Q  E      SSL + +         RK  S  +R  K  P++ +E+ S  K K+   F    K+    ++ +  G+ N           G      ++SS  K   L +   L KC +   + QCAFCLS EE+EASG MVHY++   V  D+ GG KVIH H+NCTEWAPNVYFE+D AINLEAE+SRSR+IKC  CGLKGAALGC+EKSCR++FHVPCAK   +CRWD++NFV+LCPLHA S LP E S SQ   K+   +   +        +D  S  +  +   S  K+VLCCS L+V+EKE++SEFE+++ VTVLK WDS +TH+IASTDENGAC+RTLK+L+GILEGKWIL+I+W+KAC+K  + VDE +YE+ +D+HGI+DGPRLGRLR+ NKQPKLFDG KFY MGDF PSYKG LQD++IAAGG +LHRKP+S D    L         IIYS+ELP K +P +K  I S+R+ +AEALA  T +K  SN+W+L+SI+ CKL
Sbjct:   36 CIFKSMKSASSCPVCKIPFTRREVRPAPHIDNLVTIYKNMEAASGINLFGTQNPTATKSPDDEMQGGGDAESGEKGIGGTHENHAEEEKTPTRKRSRKKLKTSTISSEKLKTSRKSPASNRAKPSFPAKKRVQVPQDILSETPLRNLKTGGSITQVNKEGTQEASIKRNVSAIGSEKRDQVKEPFFWLREEQDGERLSAQTDEDQIIEGPTPIPPSFSDLKDADDESPLKQAPSDEVQ-NKPSVDLFDSEFFEWTQKPCSPELFSSPIKTQVEDTDEIDENIEEWVAALQEKQTSVDACNTNFENPNGNQLAEALLPGLSS---EIRSSDDLNG--KKNSTKRSRKAREKSRQDHIRELNCLSDEINMDADITSKVIEEQSLDYKHKASNLKKSNKRGKKVSFVTSTESTPPIACTVSN------------ILGVQNIGDRKTAKNSYTSESKQDNEMQFPEKIAGKRQVRRSGKQRLDVVD----DPPEDLTSVQNQCKELAGSASSSLRLQMDSNRNTVNTRKRKSSLSR--KSTPSV-TERKSTKKSKLSPEFISRTKNG---EEIKPTGSTN----------PGTDVRPLNDSSKEKHCSLTDQPVLRKCVSHAKEYQCAFCLSSEESEASGPMVHYFDEKPVTADFEGGPKVIHCHRNCTEWAPNVYFEDDNAINLEAEISRSRRIKCGFCGLKGAALGCFEKSCRRSFHVPCAKWTPECRWDMENFVMLCPLHAPSLLPCESSGSQHNSKKHTAKKVKICSRK----HDTTSQSQIAHG--SHKKIVLCCSALSVQEKEVVSEFERVSKVTVLKTWDSGVTHVIASTDENGACRRTLKVLLGILEGKWILSIEWIKACMKETRPVDEERYEVNVDIHGIKDGPRLGRLRVVNKQPKLFDGYKFYFMGDFIPSYKGYLQDLVIAAGGIVLHRKPVSADQNEILPTMNQHRTLIIYSLELPAKSNPLEKDAIFSQRRCDAEALARPTCSKVASNTWILNSIAGCKL 1001          

HSP 2 Score: 64.6994 bits (156), Expect = 4.542e-7
Identity = 30/36 (83.33%), Postives = 32/36 (88.89%), Query Frame = 3

Query:  102 MGDPSHLEKMGRELKCPICLSLLNSAVSLTCNHVFC 209
            MGD   LEKMGRELKCPICL LL+SAVSLTCNH+FC
Sbjct:    1 MGD---LEKMGRELKCPICLGLLHSAVSLTCNHLFC 33          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy TrEMBL
Match: A5AT78_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_016807 PE=4 SV=1)

HSP 1 Score: 699.508 bits (1804), Expect = 0.000e+0
Identity = 443/988 (44.84%), Postives = 568/988 (57.49%), Query Frame = 2

Query:  416 MDNLVSIYKSMEVASGFNIFVTQDAP-LDTSADS--------GKRVKNDLNCC---------ERMDNQGANKGRGSREAKST--ETSDPISVKPSFPTNKRVQVPQYPLSETSMLPEKNGGGLIEVTEDESKMSSDILKEN-NLGEKGEQILSPFFWLRE-EDVERPSQLSDGDQCLYITPPGLPSFSDMKDSDDEGFSKEEVHGNRD-HLNL---FDSEMFEWTQGACSPELLSSPDKMQVADTYE----------PASQGASTAEPCNNGTCKITGHDASEEMLPNIESLRIQCVSSNKIRSCK-------SKELGKKQRKTAQKNIADRTSSHISGDHVNLGKGSEDFNKKQKDDCSSSSYL-AKTRKNCKVAGLDHHVTEPMASHFSAKAP-----KQED----TPLAAIVGMGKCGDENENLRMV-KRCRKITAXXXXXXXXXXXXXXXXXXXXDTPKEVLTVCGQANEYMIQQKSSLPVPLAHENAAEQFRKGLSKHTREVKLAPTLKSEKNSGCKKKIKVYFSDDMKSWLPEDQQQQGGNANISLERV----SEKVHGRTTVGNSESSIVKKLPLANGVALHKCETITIKIQCAFCLSMEETEASGEMVHYYEGSSVPLDYNGGSKVIHSHKNCTEWAPNVYFENDKAINLEAELSRSRKIKCSCCGLKGAALGCYEKSCRKTFHVPCAKLVSQCRWDVDNFVILCPLHASSKLPNEKSESQG-IRKRRNLRGQLLIHHNEIPINDEVSVQKKWNTCESGNKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFIIYSVELPVKCDPSKKHMILSRRQLNAEALASSTGAKAVSNSWVLSSISACKL 3202
            MD+LVSIYKSME ASG N+FVTQ+AP    S D+         ++ + D NC          ER  NQ   KG+G + +  T  E S     KPSFP  KRVQVPQYPLSET   P K  GGL E+++   K +S +L E   L + GE + SPFFWLRE EDVE  SQ  DGD  L  + P +PSFSD+K SDDE  S+    G  D   N+   FDSE+FEWTQ ACSPEL SSP +MQV D+ E          P SQ A+  E     T  +TG +  E       ++R+  +SS + ++         SK+ G+K  K  QK  A R +  + G H+N     EDF   Q  D   SS L  KT K C+ A  D++ T     + S+ +       Q+D    T L A +      DEN NL+   +RC  +  Q Q     + K QKL   + D  ++      Q N  M      + +P+A +  A    +  SKH R +      K +K     KK+KV   D  K  L +D Q+  G+  +S +      + + +    V +  S+  K L   +G AL KCE+I  KI CAFC S +++EASGEMVHY+ G  +  D+NGGS +IHSH+NCTEWAPNVYFE+  A+NL+AEL+RSR+I C CCG+KGAALGCYEKSCRK+FH PCAKL  QCRWD DNFV+LCPLHASSKLPNE S      RK+ + +GQ  I   ++ +  ++S  ++WN+  S  KLVLCCS LTV EK+I+SEFE+L+GVTVLK+W   I                                   KAC+KA + V E +YEI ID+HGIRDGPRLGRLRL NKQPKLF+G KFY  GDF PSYKG LQD++IAAGGT+LHRKPI G+      GS  +  FIIYS+ELP KC P  K+ I + R+  AEALA STGAK  SNSW L+SI+AC++
Sbjct:    1 MDSLVSIYKSMEAASGINVFVTQNAPSTKLSGDTCIPNMRMYKQQTEGDQNCGGQKADNASEERARNQRKLKGKGPKRSLKTNPEDSGLNPAKPSFPGKKRVQVPQYPLSETPKRPAKLDGGLSEMSKGGPKNNSVVLNEKPALNDNGELVFSPFFWLREDEDVENSSQQMDGDLXLSSSLPNVPSFSDIKGSDDEMPSELTPKGKVDTEFNVADPFDSEIFEWTQRACSPELCSSPMEMQVPDSDEFDEIQKGENKPDSQSATKEEEAPR-TENMTGMNCKEGT--GCAAMRLPAMSSPETKNANGQNGIGISKKRGRKSNKKGQKKRAKRGADEVLGIHINAQSVVEDFIPVQDCDKDGSSNLRKKTHKGCEKACFDNNATGAAPENVSSVSVGSKSLNQDDENIITALPASLVKKHVSDENLNLKKRGRRCANVNTQSQKGHTVRSKNQKLESAEDDMLEKGAITPNQINYDMFSHSPCVSLPMADDGKASNRGEKASKHGRIISKV-NQKRDKRLRPSKKLKVSTDDISKYGLIDDTQE--GHTKVSAKSTQPINNNQCNPEVRVLDYSSTAKKALSATSGGALRKCESIPNKISCAFCHSAQDSEASGEMVHYFNGRPIAADHNGGSNIIHSHRNCTEWAPNVYFEDGTAVNLKAELTRSRRITCCCCGIKGAALGCYEKSCRKSFHFPCAKLTPQCRWDTDNFVMLCPLHASSKLPNEISGPPAKTRKKCSTKGQSDIQRAQVAVKHDISTSQRWNSHGSPGKLVLCCSALTVAEKDIVSEFERLSGVTVLKKWGPGI-----------------------------------KACMKAKEPVAEEQYEIGIDIHGIRDGPRLGRLRLLNKQPKLFNGFKFYFFGDFMPSYKGYLQDLVIAAGGTVLHRKPILGNQETLSSGSSIYETFIIYSLELPEKCGPDMKNQIFTCRRSEAEALARSTGAKVASNSWFLNSIAACQV 947          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy TrEMBL
Match: B9GJF8_POPTR (BRCT domain-containing family protein OS=Populus trichocarpa GN=POPTR_0001s36840g PE=4 SV=1)

HSP 1 Score: 618.616 bits (1594), Expect = 0.000e+0
Identity = 310/536 (57.84%), Postives = 380/536 (70.90%), Query Frame = 2

Query: 1640 KEVLTVCGQANEYMIQQKSSLPVPLAHENAAEQFRKGLSKHTREVKLAPTLKSEKNSGCKKKIKVYFSDDMKSWLPEDQQQQGGNANISLERVSEKVHGRTTVGNSESSIVKKL-------PLA--------NGVALHKCETITIKIQCAFCLSMEETEASGEMVHYYEGSSVPLDYNGGSKVIHSHKNCTEWAPNVYFENDKAINLEAELSRSRKIKCSCCGLKGAALGCYEKSCRKTFHVPCAKLVSQCRWDVDNFVILCPLHASSKLPNEKSESQGIRKRRNLRGQLLIHHNEIPINDEVSVQKKWNTCESGNKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFIIYSVELPVKCDPSKKHMILSRRQLNAEALASSTGAKAVSNSWVLSSISACKL 3202
            KEV ++  Q ++        LP PL  +    + +    K   EVK A   +  ++  CKK ++V     ++  L  D Q+   N      + +E + G +      +  V+          LA        NG A+  C+ +   IQCAFCLS E +EASGEM+HY  G  V  DYNGGS+VIHSHKNC EWAPNVYFE D AINLEAEL+RSR+IKC CCGLKGAALGCYEKSCRK+FHVPCAKL  QCRWD +NFVILCPLHAS KLPNE  +SQ  RK    +GQ    +N++    ++++ K   +C + +KLVLCCS LTV EKEI+SEFE L+GVTVLK WDS++TH+IASTDENG C+RTLK+LMGIL+GKWI+NI+WVKACIKAMKLV+E++YEIT DVHGIRDGPR GRLR+ NKQP +F+G KFY MGDF PSYKG +QD+L+A GGTILHRKPISG  G  LL S   P FI+YS+E+P KCDPSKK+MIL+RRQ +AEALASSTGAKAVSN WVL+SI+ACKL
Sbjct:  491 KEVHSIHNQMDKDEHAHDYFLPAPLVEDKKPSEIKGKRGKQGEEVKSALCPEHNQDLKCKKNMEVSLDGIIEDGLVSDHQEGADNVFAEEAQSTENITGNSARATESAEKVQASLNTRILDDLATLRDHCQENGAAILNCK-LNYNIQCAFCLSSEVSEASGEMIHYNNGIPVAADYNGGSRVIHSHKNCAEWAPNVYFEGDNAINLEAELARSRRIKCCCCGLKGAALGCYEKSCRKSFHVPCAKLTHQCRWDTENFVILCPLHASCKLPNESKQSQERRKNCISKGQTPRQYNQVTFKHDINMHKSRKSCLTHDKLVLCCSALTVGEKEIVSEFESLSGVTVLKNWDSSVTHVIASTDENGTCRRTLKVLMGILKGKWIVNIEWVKACIKAMKLVEEMRYEITADVHGIRDGPRNGRLRVLNKQPNIFEGFKFYFMGDFIPSYKGYIQDLLVAGGGTILHRKPISGAQGTLLLDSKP-PTFIMYSLEMPDKCDPSKKNMILNRRQSDAEALASSTGAKAVSNKWVLNSIAACKL 1024          

HSP 2 Score: 270.781 bits (691), Expect = 5.197e-86
Identity = 151/269 (56.13%), Postives = 183/269 (68.03%), Query Frame = 2

Query:  329 CILKSMKSGSDCPVCKVPCRRREVRPAPHMDNLVSIYKSMEVASGFNIFVTQDAPLDTS-ADSGKRVKNDLNC--------CERMDNQGANKGRGSREAKSTETS----DPISVKPSFPTNKRVQVPQYPLSETSMLPEKNGGGLIEVTEDESKMSSDILKENN-LGEKGEQILSPFFWLREEDVERPSQLSDGDQCLYITPPGLPSFSDMKDSDDEG----FSKEEVHGNRDHL-NLFDSEMFEWTQGACSPELLSSPDKMQVADTYE 1078
            CI+KSMKSGSDCPVCKVP RRREVR APHMDNLVSIYKSME+ASGFNIFVTQ+ P  T  +D  K+V +D +C        C+ M ++     R     K  E++     PI+ KPSFPT KRVQVPQ  LS TS  PEK   G  E+ +D+ K  S + ++N  + EKGE++LSPFFWLR+ED E+ SQ +D DQ L ITPP +P+FSD+KDSDDE      SK  V GN  H+ + FDSEMFEWTQ ACSP++ SSP KMQ   T E
Sbjct:   65 CIVKSMKSGSDCPVCKVPYRRREVRAAPHMDNLVSIYKSMEIASGFNIFVTQNPPPSTKLSDVQKQVGDDTDCGREDIGWICQDMLDEQLKISRQKDSRKLDESNMELCGPITSKPSFPTKKRVQVPQCSLSGTSAQPEKLQTGSREIKKDDFKNDSSVSRKNPVVDEKGERMLSPFFWLRDEDGEKSSQHTDMDQLLDITPPNVPTFSDIKDSDDENPPESSSKGAVCGNESHVADFFDSEMFEWTQRACSPDIFSSPAKMQAEKTTE 333          

HSP 3 Score: 78.1814 bits (191), Expect = 5.197e-86
Identity = 34/37 (91.89%), Postives = 37/37 (100.00%), Query Frame = 3

Query:   99 KMGDPSHLEKMGRELKCPICLSLLNSAVSLTCNHVFC 209
            KMGDP+HLEKMGRELKCPICLSLL++AVSLTCNHVFC
Sbjct:   26 KMGDPTHLEKMGRELKCPICLSLLDTAVSLTCNHVFC 62          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy TrEMBL
Match: A0A0E0L4I8_ORYPU (Uncharacterized protein OS=Oryza punctata PE=4 SV=1)

HSP 1 Score: 610.527 bits (1573), Expect = 0.000e+0
Identity = 391/1009 (38.75%), Postives = 541/1009 (53.62%), Query Frame = 2

Query:  329 CILKSMKSGSDCPVCKVPCRRREVRPAPHMDNLVSIYKSMEVASGFNIFVTQDAPLDTSAD------SGKRVKNDLNCCERMDNQGANKGRGSREAKSTETSDPISVKPSFPTNKRVQVPQYPLSETSMLPEKNGGGLIEVTEDESKMSSDILKENN----LGEKGEQILSPFFWLREEDVERPSQLSDGDQCLYITP--PGLPSFSDMKDSDDEGFSKEEVHGNRDHLNLFDSEMFEWTQGACSPELLSSPDK--------------------MQVADTYEPASQGASTAEPCNNGTCKITGHDASEEMLPNIESLRIQCVSSNKIRSCKSKELGKKQRKTAQKNIADRTSSHISGDHVNLGKGSEDFNKKQ---KDDCSSSSYLAKTRKNCKVAGLDHHVTEPMASHFSAKAPKQEDTPLAAIVG--MGKCGDENENLRMVKRCRKITAXXXXXXXXXXXXXXXXXXXXDTPKEVLTVCGQANEYMIQQKSSLP---VPLAHENAAEQFRK-GLSKHTREVK--------LAPTLKSEKNSGCKKKIKVYFSDDMKSWLPEDQQQQGGNANISLERVSEKVHGRTTVGNSESSIVKKLPLANGVALHKCETITIKIQCAFCLSMEETEASGEMVHYYEGSSVPLDYNGGSKVIHSHKNCTEWAPNVYFENDKAINLEAELSRSRKIKCSCCGLKGAALGCYEKSCRKTFHVPCAKLVSQCRWDVDNFVILCPLHASSKLPNEKSESQGIRKRRNLRGQL--LIHHNEIPINDEVSVQKKWNTCESGNKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFIIYSVELPVKCDPSKKHMILSRRQLNAEALASSTGAKAVSNSWVLSSISACKL 3202
            C+ +SMKS S CPVCKVP RRRE+RPAPHMDNLVSI+KSME A+G N+  TQ+AP    AD      SGK  K       R     + K + + +  +   S P + KPSF TNKR+QV  +P SET +  E+    +++  E ++  + D+ +  N      + G   LSPFFWLRE++ E  +  +  +     TP     PSFSD+KDSDDE       +       LFDSE+FEWTQ  CSPEL S+P K                    M +  +++     ++ A+  N    K T     +    N  + ++  ++   I+   + + G  +RKTA      + SS I G + +  +     N K      D S  SYL K  +   V   D  ++E   +      PK     L  I G  M    ++N   R  KR R+++                        K V    G  NE  I Q  +L    +     NA  Q +  G  K   E++        +AP +   K   C+ K  VY    +++   +++Q +                G    GN     ++  P A  V L KCE  T+K+ CAFC +   TE SG MVHY  G  VP ++NGG+  +HSHKNC EWAP+VYFE+D   NL  EL+RSR+IKC+CCG+KGAALGC+E SCR++FH  CAKL+ +CRWD +NFV+LCPLH SSKLPNE SE Q   KR+     +      ++I  N +     KW +  S  K VLCCS L+  EKE++SEF KLAGV +   W SN+TH+IASTD +GACKRTLK LM IL G+WI++I WVK C++ M+ +DE K+E+T DVHGI DGPRLGR R+ ++QPKLFDG +FY+ GD+  SY+G LQD+++AAGGT+L RKP+S D    L  S    + I+YS+E   +     +     RRQ +A+AL  ++G + VS++WV+ SI+AC L
Sbjct:   39 CLTESMKSASTCPVCKVPFRRREMRPAPHMDNLVSIFKSMEAATGTNVVSTQEAPAVKVADGSDCVNSGKHSKRSQKPLARKRKVSSEKEKNTAKDAAASASHP-TTKPSFSTNKRIQVRPFPESETPIRVER----IMKPGEPKNNRNDDVEEGANKAVASAQPGSPSLSPFFWLREQEQEGCTAETLSETLSLDTPLRHNAPSFSDIKDSDDEIPLNTTPNSKAGATELFDSEIFEWTQRPCSPELYSTPLKKQSKAKNKLDQIEEKGDEEDMHIGGSFDKLGHASNAAQLVNT---KATKQKRKKTSPTNKNNAKLSNLAEPCIKKSDANQQGSNRRKTAALKSCQKNSSAI-GRNTSGRRNKASSNSKPIHCSSDYSPESYLPK--EGLDVEAPDKPLSERTQNLEKNSQPKGSARKLE-IAGKTMADTAEKNSEPRS-KRARRMSDHAIA-------------------KPVEVPSGSGNEAEIPQLHTLAKGSIQRKSSNARRQSKICGEQKSKNELENTIPNTATMAPIILHGK---CQNKEAVYTDPSVRTASVKNKQAKFSK--------RPDCFGTENFGN-----LQACP-ARNVLLKKCEVSTLKVSCAFCQTDVITEESGVMVHYQNGKQVPAEFNGGA--VHSHKNCLEWAPDVYFEDDSVFNLTTELARSRRIKCACCGIKGAALGCFEMSCRRSFHFTCAKLIPECRWDNENFVMLCPLHRSSKLPNENSEQQKQPKRKTTFKGITGFSRPSQIGSNQDCGNNWKWPS-GSPRKWVLCCSSLSGSEKELVSEFAKLAGVPISATWSSNVTHVIASTDLSGACKRTLKFLMAILNGRWIVSIDWVKTCMECMEPIDEHKFEVTTDVHGITDGPRLGRCRVIDRQPKLFDGMRFYLHGDYTKSYRGYLQDLVVAAGGTVLQRKPVSRDQQKLLDDSSD--LLIVYSLENQDRAKSKAETKAADRRQADAQALTCASGGRVVSSAWVIDSIAACNL 993          

HSP 2 Score: 67.0106 bits (162), Expect = 9.023e-8
Identity = 29/36 (80.56%), Postives = 32/36 (88.89%), Query Frame = 3

Query:  102 MGDPSHLEKMGRELKCPICLSLLNSAVSLTCNHVFC 209
            M D   LEKMGRELKCPICLSLL+SAVS++CNHVFC
Sbjct:    1 MADMGSLEKMGRELKCPICLSLLSSAVSISCNHVFC 36          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy TrEMBL
Match: A0A067GIX5_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g001785mg PE=4 SV=1)

HSP 1 Score: 547.354 bits (1409), Expect = 2.162e-174
Identity = 353/780 (45.26%), Postives = 474/780 (60.77%), Query Frame = 2

Query:  323 SVCILKSMKSGSDCPVCKVPCRRREVRPAPHMDNLVSIYKSMEVASGFNIFVTQD-APLDTSAD--SGKRVKNDLNCCERMDNQGANKG-RGSREAKST-ETSDPISVKPSFPTNKRVQVPQYPLSETSMLPEKNGGGLIEVTEDESKMSSDILKENNLGEKGEQILSPFFWLR-EEDVERPSQLSDGDQCLYITPPGLPSFSDMKDSDDEGFSK----EEVHGNRDHLNLFDSEMFEWTQGACSPELLSSPDKMQVADT------YEPASQGASTAEPCNNG--TCKITGHDASEEMLPNIESLRIQCVS-SNKIRSCKSKELGKKQRKTAQKNIADRTSSHISGDHVNLGKGSEDFNKKQKDDCSSSSYLAKT---RKNCKVAGL-DHHVTEPMASHFSAKAPKQEDTPLAAIVGMGKCGDENEN-LRMVKRCRKITAXXXXXXXXXXXXXXXXXXXXDTPKEVLTVCGQANEYMIQQKSSLPVPLAHENAAEQFRKGLSKHTREVKL-APTLKSEKNSGCKKKIKVYFSDDMKSWL-PEDQQQQGGNANISLERVSEKVHGRTTVGNSESSIVKKLPLANGVALHKCETITIKIQCAFCLSMEETEASGEMVHYYEGSSVPLDYNGGSKVIHSHKNCTEWAPNVYFENDKAINLEAELSRSRKIKCSCCGLKGAALGCYEKSCRKTFHVPCAKLVSQCRWDVDNFVILCPLHASSKLPNEKSESQGIRKRRNLRGQLLIHHNEIPINDEVSVQKKWNTCESGNKLVLCCSGLTVEEK 2584
            + CI+KSMKSGS+CPVCKVP  RRE+R APHMDNLVS+YKSMEVASG NIFVTQD +   +S D   G++V     C +++++QG +KG R  R +K+T E S  +  KPS PT KRVQV QYPL ET   P K  GG ++ T  E K SS +++EN +  K   +LSPFFWLR EED E+ SQ ++ D   YITPP +PSFSD+KDS+D   S+     EVHG  D L+ F+SE+FEWTQ ACSPELL SP K QVAD        E A QG++T    +NG  TC I       E     ++L  +  + +N I + K  + G+K   TAQK  A R ++  S       + SE+F +KQ    S++ +  K+   R  C    + D+ +   + +    +  K   + L   +G  +  DE+ + ++  K+C KI +Q +   C+K K++K+   +V+  + V  V  +  +  + Q S +P      +  E   KG +  T +    +  LK +K     K++K  FS   K  L  E + Q+G ++  +  +  E V G + +G     +++KL      AL KCET+  KIQC+FC S E +EASGEMVHYY G  V  DYNGGSK+IHSH+NCTEWAPNVYFE+D  INLEAEL+RSR+IKC CCGLKGAALGCYEK+CRK+FHVPCAKL+ QCRWD DNFV+LCPLH SS LPNE   SQ  RK + +  +LL  HN++    ++S    W + E  NKLVLCCS LTV E+
Sbjct:   38 NACIVKSMKSGSNCPVCKVPYHRREIRAAPHMDNLVSVYKSMEVASGINIFVTQDESSTKSSGDLICGEQVTG--GCQDKVEHQGTSKGKRFRRTSKATVEPSGTVCTKPSCPTKKRVQVAQYPLPETPTQPAKLEGGTVKNTTGELKSSSVVIRENPVINKEGTVLSPFFWLRDEEDREKSSQHTETD--AYITPPDVPSFSDLKDSEDISSSELSPTAEVHGKSDGLDFFESEIFEWTQRACSPELLPSPAKTQVADAEENGKGLEAALQGSNTVVDISNGNATCVIFEQGNGNENEGRCKALCPRNTNPNNMIENGKLNKKGRKANSTAQKKYAKRDTNKGSTTPATSNEESENFVQKQTS-ISNAKFSQKSSNRRGTCAAEPIPDNVLAASVRTEIVDQHGKNIGSDLPTSLGKKQGSDEDLHFMKTRKKCWKINSQTEEDFCTKSKRRKVDSTEVNVLENVSVVQKKTYKDTVPQPSLIPT-FEDADKKESLLKGKTTKTAKKTKTSSNLKCDKELQWNKRMKASFSGISKDGLVGESKVQEGCSSTANETQSPEGVRGSSDIG-----VLEKLHKTQRGALRKCETLAHKIQCSFCHSSENSEASGEMVHYYNGKPVSADYNGGSKIIHSHRNCTEWAPNVYFEDDTVINLEAELARSRRIKCCCCGLKGAALGCYEKTCRKSFHVPCAKLILQCRWDTDNFVMLCPLHTSSNLPNENPGSQESRK-KCISKKLLTQHNKVAFKSDISTN-SWQSWE--NKLVLCCSALTVGEQ 802          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy TrEMBL
Match: A0A0B2PH80_GLYSO (Protein BREAST CANCER SUSCEPTIBILITY 1 like OS=Glycine soja GN=glysoja_032801 PE=4 SV=1)

HSP 1 Score: 540.036 bits (1390), Expect = 1.722e-169
Identity = 260/431 (60.32%), Postives = 319/431 (74.01%), Query Frame = 2

Query: 1922 EKVHGRTTVGN-SESSIVKKLPLANGVALHKCETITIKIQCAFCLSMEETEASGEMVHYYEGSSVPLDYNGGSKVIHSHKNCTEWAPNVYFENDKAINLEAELSRSRKIKCSCCGLKGAALGCYEKSCRKTFHVPCAKLVSQCRWDVDNFVILCPLHASSKLPNEKSESQGIRKR---RNLRGQLLIHHNEIPINDEVSVQKKWNTCESGNKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFIIYSVELPVKCDPSKKHMILSRRQLNAEALASSTGAKAVSNSWVLSSISACKL 3202
            E +H    V + +++S  K  PL +  AL KCE+   K QC FCLS EE+E SG MVHY +G  V  DY GGSKV H H+NCTEWAPNVYF+ D AINLEAE+SRSR+IKCS CGLKGAALGCYEKSCR++FHVPCA   SQCRWD  NFV+LCPLHASS LP E S SQ   K+      +   L H      N   +  + +       K+VLCCS L+V+E+E++SEFE+++   VLK WDS++TH+IASTDENGAC+RTLK+L+GILEGKWILNI+W+KAC+K M  VDE +YEI +D+HGIRDGPRLGRLR+ NKQPKLF G KFY+MGDF PSYKG LQD+L+AAGG ILHRKP+SGD  +    +  +   IIYS+ELP KC P KK  I S+R+ +AE LASSTG+K  SN+W+L+SI+ACKL
Sbjct:  559 ESIHQGPDVRDLNDTSKEKHCPLMDQTALRKCESHVTKYQCIFCLSSEESETSGPMVHYLDGRPVTADYEGGSKVTHCHRNCTEWAPNVYFDGDNAINLEAEISRSRRIKCSFCGLKGAALGCYEKSCRRSFHVPCANWTSQCRWDTQNFVMLCPLHASSMLPCEGSGSQKRSKKCAASEGKNHGLKHDT---TNQSRAAHRSYK------KIVLCCSALSVQEREVVSEFERVSKAAVLKNWDSSVTHVIASTDENGACRRTLKVLLGILEGKWILNIEWIKACMKEMGPVDEERYEINVDIHGIRDGPRLGRLRVLNKQPKLFYGYKFYVMGDFIPSYKGYLQDLLVAAGGIILHRKPVSGDQESTSPDTHPYQTLIIYSLELPDKCKPLKKDTICSQRRHDAEVLASSTGSKVASNTWILNSIAACKL 980          

HSP 2 Score: 234.572 bits (597), Expect = 9.059e-69
Identity = 139/270 (51.48%), Postives = 168/270 (62.22%), Query Frame = 2

Query:  329 CILKSMKSGSDCPVCKVPCRRREVRPAPHMDNLVSIYKSMEVASGFNIFVTQDAPLDTSADSGKRVKNDLNCCERMDNQGANKGRGSREAK------------STETSDPISVKPSFPTNKRVQVPQYPLSETSMLPEKNGGGLIEVTEDESKMSSDILKENNLG---EKGEQILSPFFWLREE-DVERPSQLSDGDQCLYITPPGLPSFSDMKDSDDEGFSK----EEVHGNRDHLNLFDSEMFEWTQGACSPELLSSPDKMQVADTYE 1078
            C+ KSMKS S CPVCK+P  RREVRPAPHMDNLVSIYK+ME ASG NIF TQ+AP+   +D  K+ + D N   +M+  G + GR   +              STE+S     KPSFP  KRVQVPQ  LSET M   K G  L E+ +++       + E+      EK   +LSPFFWLREE D ER SQ +D D+ +  + P  PSFSD+KDSDDE  S     +EV  N+  +NLFDSEMFEWTQ  C+PEL SSP KMQV DTYE
Sbjct:   36 CVFKSMKSASACPVCKIPFTRREVRPAPHMDNLVSIYKNMEAASGVNIFETQNAPVTNLSDGEKQCEVDAN-SGKMEAGGTHTGRPREKKTRKKKESKKTVQASTESSGSDFAKPSFPAKKRVQVPQNLLSETPMKSLKIGVSLSEINKEKEGTEKVPVMESERPLQREKDNPVLSPFFWLREEKDGERLSQQTDDDEIIDGSTPNPPSFSDLKDSDDEIPSNVAPMDEVQ-NKGSVNLFDSEMFEWTQRPCTPELFSSPSKMQVMDTYE 303          

HSP 3 Score: 56.9954 bits (136), Expect = 9.059e-69
Identity = 27/36 (75.00%), Postives = 30/36 (83.33%), Query Frame = 3

Query:  102 MGDPSHLEKMGRELKCPICLSLLNSAVSLTCNHVFC 209
            MGD   LE+M REL CPIC SLL+SAVSLTCNH+FC
Sbjct:    1 MGD---LERMARELICPICWSLLDSAVSLTCNHLFC 33          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy TrEMBL
Match: V7BTN7_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_006G167200g PE=4 SV=1)

HSP 1 Score: 535.413 bits (1378), Expect = 2.120e-166
Identity = 255/416 (61.30%), Postives = 312/416 (75.00%), Query Frame = 2

Query: 1955 SESSIVKKLPLANGVALHKCETITIKIQCAFCLSMEETEASGEMVHYYEGSSVPLDYNGGSKVIHSHKNCTEWAPNVYFENDKAINLEAELSRSRKIKCSCCGLKGAALGCYEKSCRKTFHVPCAKLVSQCRWDVDNFVILCPLHASSKLPNEKSESQGIRKRRNLRGQLLIHHNEIPINDEVSVQKKWNTCESGNKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFIIYSVELPVKCDPSKKHMILSRRQLNAEALASSTGAKAVSNSWVLSSISACKL 3202
            +++S  K   L +   L KCE++    QC FCLS EE+E SG MVHY +G  VP DY GG KV H H+NCTEWAPNVYF+ D AINLEAE+SRSR+I+CS CGLKGAALGCYEKSCR++FHVPCAK  S CRWD  NFV+LCPLHASS LP E S SQ     R+ +G      N  P  D  + ++      S  K+VLCCS L+V+EKEI+S+FE ++ VTVLK WDS++TH+IAST+ENGAC+RTLK+LMGILEGKWIL ++W+KAC+K M  V E +YEI +DVHGIRDGPRLGRLR+ NKQPKLFDG KFY MGDF PSYKG LQD+++AAGG ILHRKP+SGD  + LL +  +   IIYS+ELP KC PS    I  +R  +AE LASSTG+K  +N+W+L+SI+ACKL
Sbjct:  687 NDASKEKHCSLTDKTVLRKCESLVKSYQCLFCLSSEESEVSGPMVHYLDGKPVPADYEGGFKVTHCHRNCTEWAPNVYFDGDNAINLEAEISRSRRIRCSFCGLKGAALGCYEKSCRRSFHVPCAKWTSLCRWDTQNFVMLCPLHASSMLPCEGSGSQ----ERSKKGPGRDGKNHGPSLD--TTRQTRADHRSYKKIVLCCSALSVQEKEIVSKFESVSKVTVLKNWDSSVTHVIASTEENGACRRTLKVLMGILEGKWILKVEWIKACMKEMNPVGEERYEINVDVHGIRDGPRLGRLRVLNKQPKLFDGYKFYFMGDFIPSYKGYLQDLVVAAGGIILHRKPVSGDQKSMLLDTHPYQTLIIYSLELPDKCKPSNTDTICRQRCHDAEVLASSTGSKVATNTWILNSIAACKL 1096          

HSP 2 Score: 221.476 bits (563), Expect = 9.949e-66
Identity = 137/275 (49.82%), Postives = 160/275 (58.18%), Query Frame = 2

Query:  329 CILKSMKSGSDCPVCKVPCRRREVRPAPHMDNLVSIYKSMEVASGFNIFVTQDAPLDT------SADSGKRVKNDLNCCERMDNQGANKGR------------GSREAKSTETSDPISVKPSFPTNKRVQVPQYPLSETSMLPEKNGGGLIEVTEDESKMSSDILKENNLG---EKGEQILSPFFWLREE-DVERPSQLSDGDQCLYITPPGLPSFSDMKDSDDEGFSKEEVHGNRDH---LNLFDSEMFEWTQGACSPELLSSPDKMQVADTYE 1078
            CI+KSMKS   CPVCK+P  RREVRPAPHMDNLVSIYK+MEVASG NIFVTQ+   D       +ADSGK           ++  G++KG                   + E+S     KPSFP  KRV VPQ  LSET M   K G  L E  +++  +    + E+      EK   +LSPFFWLREE D E  SQ +D DQ +  + P  PSFSD+KDSDDE  S       R H   +NLFDSEMFEWTQ  CSPEL SSP KMQV DTYE
Sbjct:   36 CIVKSMKSAFACPVCKIPFTRREVRPAPHMDNLVSIYKNMEVASGINIFVTQNVSQDVEKQCEGNADSGK-----------VEAGGSHKGHVQEKKTRKIKKVKKTVQTNMESSGSGFAKPSFPAKKRVLVPQNILSETPMKNLKLGDSLNEFNKEKEGVEKVSVMESERPLQIEKSVPVLSPFFWLREEKDGETLSQPADEDQIIDCSTPNPPSFSDLKDSDDEHTSNVAPFDERQHQISVNLFDSEMFEWTQRPCSPELFSSPSKMQVMDTYE 299          

HSP 3 Score: 59.6918 bits (143), Expect = 9.949e-66
Identity = 27/36 (75.00%), Postives = 31/36 (86.11%), Query Frame = 3

Query:  102 MGDPSHLEKMGRELKCPICLSLLNSAVSLTCNHVFC 209
            MGD   LE+MG+ELKCPIC SL +SAVSLTCNH+FC
Sbjct:    1 MGD---LERMGKELKCPICWSLFDSAVSLTCNHLFC 33          

BLAST of g.barbadense_cottongen_reftransV1_0098060 vs. ExPASy TrEMBL
Match: V7BSA9_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_006G167200g PE=4 SV=1)

HSP 1 Score: 535.028 bits (1377), Expect = 2.553e-166
Identity = 255/416 (61.30%), Postives = 312/416 (75.00%), Query Frame = 2

Query: 1955 SESSIVKKLPLANGVALHKCETITIKIQCAFCLSMEETEASGEMVHYYEGSSVPLDYNGGSKVIHSHKNCTEWAPNVYFENDKAINLEAELSRSRKIKCSCCGLKGAALGCYEKSCRKTFHVPCAKLVSQCRWDVDNFVILCPLHASSKLPNEKSESQGIRKRRNLRGQLLIHHNEIPINDEVSVQKKWNTCESGNKLVLCCSGLTVEEKEIMSEFEKLAGVTVLKRWDSNITHIIASTDENGACKRTLKILMGILEGKWILNIKWVKACIKAMKLVDELKYEITIDVHGIRDGPRLGRLRLQNKQPKLFDGCKFYIMGDFEPSYKGDLQDVLIAAGGTILHRKPISGDPGARLLGSPAFPIFIIYSVELPVKCDPSKKHMILSRRQLNAEALASSTGAKAVSNSWVLSSISACKL 3202
            +++S  K   L +   L KCE++    QC FCLS EE+E SG MVHY +G  VP DY GG KV H H+NCTEWAPNVYF+ D AINLEAE+SRSR+I+CS CGLKGAALGCYEKSCR++FHVPCAK  S CRWD  NFV+LCPLHASS LP E S SQ     R+ +G      N  P  D  + ++      S  K+VLCCS L+V+EKEI+S+FE ++ VTVLK WDS++TH+IAST+ENGAC+RTLK+LMGILEGKWIL ++W+KAC+K M  V E +YEI +DVHGIRDGPRLGRLR+ NKQPKLFDG KFY MGDF PSYKG LQD+++AAGG ILHRKP+SGD  + LL +  +   IIYS+ELP KC PS    I  +R  +AE LASSTG+K  +N+W+L+SI+ACKL
Sbjct:  689 NDASKEKHCSLTDKTVLRKCESLVKSYQCLFCLSSEESEVSGPMVHYLDGKPVPADYEGGFKVTHCHRNCTEWAPNVYFDGDNAINLEAEISRSRRIRCSFCGLKGAALGCYEKSCRRSFHVPCAKWTSLCRWDTQNFVMLCPLHASSMLPCEGSGSQ----ERSKKGPGRDGKNHGPSLD--TTRQTRADHRSYKKIVLCCSALSVQEKEIVSKFESVSKVTVLKNWDSSVTHVIASTEENGACRRTLKVLMGILEGKWILKVEWIKACMKEMNPVGEERYEINVDVHGIRDGPRLGRLRVLNKQPKLFDGYKFYFMGDFIPSYKGYLQDLVVAAGGIILHRKPVSGDQKSMLLDTHPYQTLIIYSLELPDKCKPSNTDTICRQRCHDAEVLASSTGSKVATNTWILNSIAACKL 1098          

HSP 2 Score: 216.468 bits (550), Expect = 3.351e-64
Identity = 137/277 (49.46%), Postives = 160/277 (57.76%), Query Frame = 2

Query:  329 CILKSMKSGSDCPVCKVPCRRREVRPAPHMDNLVSIYKSMEVASGFNIFVTQDAPLDT------SADSGKRVKNDLNCCERMDNQGANKGR------------GSREAKSTETSDPISVKPSFPTNKRVQVPQYPLSETSMLPEKNGGGLIEVTEDESKMSSDILKENNLG---EKGEQILSPFFWLREE-DVERPSQLSDGDQCLYITPPGLPSFSDMKDSDDEGFSKEEVHGNRDH---LNLFDSEMFEWTQGACSPELLSSPDKM--QVADTYE 1078
            CI+KSMKS   CPVCK+P  RREVRPAPHMDNLVSIYK+MEVASG NIFVTQ+   D       +ADSGK           ++  G++KG                   + E+S     KPSFP  KRV VPQ  LSET M   K G  L E  +++  +    + E+      EK   +LSPFFWLREE D E  SQ +D DQ +  + P  PSFSD+KDSDDE  S       R H   +NLFDSEMFEWTQ  CSPEL SSP KM  QV DTYE
Sbjct:   36 CIVKSMKSAFACPVCKIPFTRREVRPAPHMDNLVSIYKNMEVASGINIFVTQNVSQDVEKQCEGNADSGK-----------VEAGGSHKGHVQEKKTRKIKKVKKTVQTNMESSGSGFAKPSFPAKKRVLVPQNILSETPMKNLKLGDSLNEFNKEKEGVEKVSVMESERPLQIEKSVPVLSPFFWLREEKDGETLSQPADEDQIIDCSTPNPPSFSDLKDSDDEHTSNVAPFDERQHQISVNLFDSEMFEWTQRPCSPELFSSPSKMQLQVMDTYE 301          

HSP 3 Score: 59.6918 bits (143), Expect = 3.351e-64
Identity = 27/36 (75.00%), Postives = 31/36 (86.11%), Query Frame = 3

Query:  102 MGDPSHLEKMGRELKCPICLSLLNSAVSLTCNHVFC 209
            MGD   LE+MG+ELKCPIC SL +SAVSLTCNH+FC
Sbjct:    1 MGD---LERMGKELKCPICWSLFDSAVSLTCNHLFC 33