g.barbadense_cottongen_reftransV1_0097827, g.barbadense_cottongen_reftransV1_0097827 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy Swiss-Prot
Match: SBT35_ARATH (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 405.216 bits (1040), Expect = 2.902e-125
Identity = 283/754 (37.53%), Postives = 403/754 (53.45%), Query Frame = 3

Query:  522 DRSFIHSSTEIFLVTFSGREERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLD---DNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYY-NVMHASN-GNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVC------SEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNF-LKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSA------INFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEK-VKGKIIVCFDG---FPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPV--TNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTKFKCPTKSSDDLXXXXXXXXXXXXKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGS-EASSGYNFGSITWSAAQYSVRTVFAVNVE 2708
            D  F+  S    L +  G +     S+   Y H   GF+A LT+ +A +L+    VV V  DS  +L TTR+WD+L     N N  LN  +         VIIG IDTG+W ES SF D G+G IP  WKG C  G  F  ++CNRKLIGA+Y+ N   A N G NTT+ R   S RD +GHGTHTASIA G+ V N SY GLA G  RGG+P  RIA YK C          C  S ILKA+D+++ DGVD++S+S+G    L P   L+D IA GAFHA   G++V C+GGN GP   TV N APWI TVAA+ +DR F + + LGN ++  G A      + F++L   E           A ++    +  C   +L+P + + GK+++CF     F  VSR    + A  A   G+I+      +       FP   +   +G  +L YI S ++P   I P+  + G      VA FSSRGP+ ++  ILKPDI APGV+IL A +P D   S       GG+ + +GTSMA P V G  AL+K++HP W+ +  RSA++TTA   D   + +     S   A P + G G ++P KA +PGL+++    DY+ +LC  G  + +I  +      C T  +  L  N+   +I   K E     +T+ R+VTNVG  +  Y   V+ P+G++V V P+ + F    K VSF V    + + ++G+ FG++ W+ + ++V    +V  +
Sbjct:   41 DPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLSVANPNNLLNDTNMGDQ-----VIIGFIDTGVWPESESFNDNGVGPIPSHWKGGCESGEKFISTNCNRKLIGAKYFINGFLAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGGAPRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGNRKVILGQALYTGQELGFTSLVYPEN----------AGFTNETFSGVCERLNLNPNRTMAGKVVLCFTTNTLFTAVSRAASYVKA--AGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRSTRSPVVKIQPSRTLVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILAATSP-DSNSSV------GGFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVNPEKAADPGLIYDMGPRDYILYLCSAGYNDSSITQLVGNVTVCSTPKTSVLDVNLPSITIPDLKDE-----VTLTRTVTNVGTVDSVYKVVVEPPLGIQVVVAPETLVFNSKTKNVSFTVRVSTTHKINTGFYFGNLIWTDSMHNVTIPVSVRTQ 765          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy Swiss-Prot
Match: SBT4E_ARATH (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 402.134 bits (1032), Expect = 2.819e-124
Identity = 280/721 (38.83%), Postives = 399/721 (55.34%), Query Frame = 3

Query:  561 VTFSGREERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVC-SEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINEN--DESAPFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVT---NGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTK-FKC----PTKSSDDLXXXXXXXXXXXXKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNF-GSITWSAAQYSVRT 2687
            +  S  E +ER   +  Y  A   F+A L+  EA ++     VVSV  +   +LHTT+SWDF+       L    +L  K+  DVIIG++DTGI  +S SF D G+G  P +WKG C  GP    + CN K+IGA+Y+      +  N     V +SP D  GHGTHT+S  AG +VANAS +G+A GTARG  P  R+A YKVC +  GC    IL   + A+ DGV+IISI   +      ++  D I++G+FHA + G++   S GN+GP   TVTN  PWI TVAAS IDR F SK+ LGNG+ F G  I+  +  + ++YPL  G D A        AR C+  SLD +KVKGK++VC  G   V  TIK          G I++++   D +  F +   P T V +++G  I +YINS ++ SA+I  T ++    PAP VA FSSRGP+  +  +LKPDI APG+ IL A T K          +   + + SGTSMACPHV G +A VKS HP WT + I+SA++T+A       KP++   N  A FA     G G+I+P +A +PGLV++     Y++FLC  G    T+  +  T+   C    P    D L       ++  +K          +R VTNVG P+  YT+TV+AP G+++ V P+ ++F +  ++ SFKV+    + + G    G + W + ++SVR+
Sbjct:   63 LNISQEEAKERKVYS--YTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFV----GLPLTAKRHL--KAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSC--GPYKNFTGCNNKIIGAKYFK-----HDGNVPAGEV-RSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAGFEAAIHDGVEIISI---SIGGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFS-PKAKSYPLVSGVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSY-----GGAGAIIVSDQYLDNAQIFMA---PATSVNSSVGDIIYRYINSTRSASAVIQKTRQV--TIPAPFVASFSSRGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKSFHPDWTPAAIKSAIITSA-------KPISRRVNKDAEFA----YGGGQINPRRAASPGLVYDMDDISYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTST---LAVFRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWKSPRHSVRS 739          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy Swiss-Prot
Match: SBT25_ARATH (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 1.040e-81
Identity = 249/738 (33.74%), Postives = 357/738 (48.37%), Query Frame = 3

Query:  612 YNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEG---MGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSED-GCPGSTILKAIDDAVKDGVDIISISIG--------TSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTR-TETYPLAYGKDI-----AAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVV--SRTIKKLVA--EDAKAKGLILINEN-DESAPFDSGPFPFTEVGTTI-----GYKILKYINSN------------KNPSAI---ILPTVEIPGIKPAPVVAYFSSRGP-----SVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSAS--------FATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYN-GSPEKTIRSMSKTKFKCPTKSSDDLXXXXXXXXXXXXKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSI 2654
            Y H + GF+A ++ ++A  L    GV SV  D  ++  TT + +FL   ++     G +   ++  D++IG +D+GI+   PSF        G +P  +KG C E P  KKS CNRK++GA+++     + G     +    SP D  GHG+HTA+IAAG        HG   G A G +P  RIA YK      G   + ++ AID AV DGVDI+S+S+G         + FL P    D   +GA  A   GV VA + GN GP P T+ + +PWI TVAA+  DR + + + LGNG++  G  +  S  TR    Y L    D+      +KY+P    R   P   + + V+G I++C   F  V  + +IKK+VA  +   A G +L+ EN      FD  P P    G  I        ++ Y N++            K   +I   + P +     K AP VA FS+RGP     S    ++LKPDI+APG  I  A  P   +E    G    G+AL SGTSMA PH+ G +ALVK  HP+W+ + I+SALMTT+TV D   + +     S         ATP + G G ++P  AL+PGL+F+   EDYL FLC   G     IR+ + T      K   +  +           +   +G  T+ R VTNV     TYT T +    + ++V P  +T      R +F V       S  Y+FG +
Sbjct:   89 YKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLGLPTDVWPTGGGF--DRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLP-HYKGKCEEDPHTKKSFCNRKIVGAQHFAEAAKAAGAFNPDIDY-ASPMDGDGHGSHTAAIAAGNNGIPLRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPTTTKTTFLNPF---DATLLGAVKA---GVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLTLGNGKMLAG--MGLSPPTRPHRLYTLVSANDVLLDSSVSKYNPSDCQR---PEVFNKKLVEGNILLCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFD--PVPSAIPGILITDVSKSMDLIDYYNASTSRDWTGRVKSFKAEGSIGDGLAPVLH----KSAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYVG---EGFALISGTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVNPSAALDPGLIFDAGYEDYLGFLCTTPGISAHEIRNYTNTACNYDMKHPSNFNAPSIA-------VSHLVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNPPAMTLRPGATR-TFSVTMTVRSVSGVYSFGEV 794          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy Swiss-Prot
Match: SBT24_ARATH (Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana GN=SBT2.4 PE=1 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 1.166e-64
Identity = 227/741 (30.63%), Postives = 341/741 (46.02%), Query Frame = 3

Query:  612 YNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGM-----GEIPK-RWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFH-----AEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAK---------YSPIP-EARSCY-PGSLDPEKVKGKIIVCF--DGFPVVSRTIKKL--VAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTI-----GYKILKY-----------INSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTE-----NILKPDIMAPGVAILGAITPKDEEESASDGVKPG-GYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVT----NGSASFATPH-EMGVGEISPVKALNPGLVFETTTEDYLRFLCY--NGSPEKTIRSMSKTKFKCPTKSSDDLXXXXXXXXXXXXKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAA 2669
            + H +   +   T  +A +L    GV +V  D  ++L TT + DFL+          +    ++  D++IG +DTGI    PSF    +       + +  + G C  GP F    CN K+I AR+++    ++G   + + +  SP D  GHG+H ASIAAG         G   G A G +P  RIA YK           ++ AID A+ DGVD++++S+G     +P   K P  +G F      A + GV V  + GN GP P +V + +PW+  VAA N DR + + ++L  G+  QG  ++   L      PL   + + AK           P+  +   C  P + DP  V G I++C   DGF     T+  +   A      G ILI  N     + + P  F+  G  I        IL+Y           + +     A I           APVV+ FSSRGP+ +       ++LKPDI+APG  I GA +      SA D +  G  +A+ SGTSMA PH+ G  AL+K ++P WT +MI SA+ TTA  YD+  + ++      S  F + H + G G ++P +AL+PGLV     EDY+ FLC   N SP  TIR    T   C T  S     N    +IS  K       L ++RS  +V +   TY  +V  P G  V++ P   T +   K     + F+ ++  + + FG +  + +
Sbjct:  101 FKHVINAIAVRTTASQAKKLGKTKGVKAVEEDKGVKLMTTYTPDFLELPQQVWQKISNEGDRRAGEDIVIGFVDTGINPTHPSFAALDLTNPYSSNLSRLHFSGDCEIGPFFPPGSCNGKIISARFFSAGARASGALNSSLDI-LSPFDASGHGSHVASIAAGNAGVPVIVDGFFYGRASGMAPRSRIAVYKAIYPSIGTLVDVIAAIDQAIMDGVDVLTLSVGPD---EPPVDK-PTVLGIFDLAMLLARKAGVFVVQAVGNNGPSPSSVLSYSPWVVGVAAGNTDRSYPAPLILDGGQTVQGVGLSGPTLGA----PLVQHRLVLAKDAVRTNGSVLQPLTRDIEECQRPENFDPAAVFGSIVICTFSDGFYNQMSTVLAITQTARTLGFMGFILI-ANPRFGDYVAEPVIFSAPGILIPTVSAAQIILRYYEEKTFRDTRGVATQFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATRSPLDVLKPDILAPGHQIWGAWS----LPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLNPSWTPAMIASAISTTANEYDSNGEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLVLPAGFEDYISFLCSLPNISP-ATIR--DATGVLCTTTLSHPANLNHPSVTISALKES-----LVVRRSFQDVSNKTETYLGSVLPPNGTTVRLTPTWFT-VPPQKTQDLDIEFNVTQVLNKFTFGEVVLTGS 818          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy Swiss-Prot
Match: SUBV_BACSU (Minor extracellular protease vpr OS=Bacillus subtilis (strain 168) GN=vpr PE=1 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 2.566e-23
Identity = 147/603 (24.38%), Postives = 248/603 (41.13%), Query Frame = 3

Query:  600 ITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFL--DDNSNPKLNHGS-YLPHKSTYD-------VIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPR-DTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGST--ILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTN--AAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAA---KYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILIN----ENDESAPFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHE-MGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEK 2339
            +   Y     GFS  L  +E  +L     V +V+P+   +    +  D    +D  +P+++  + Y+     +D       + + +IDTG+    P  K +  G+          +G DF  +D          Y+      G+          PR +   HGTH A   A            A GT +G +P   + AY+V    G  G+T  ++  ++ AV+DG D++++S+G S    P++     +     A   GV+   S GN GP+ +TV +   +    +V A+ +    +              A+ F + +  +        D+ A   K   + EA        + + + GK+ V   G   ++   K   A+ A A G+++ N    E + + P  S   P  ++    G K++  + + +  +   L   +  G +    VA FSSRGP V+   ++KPDI APGV I+  I   D +        P GY  + GTSMA PH+ GA A++K   PKW+   I++A+M TA    +    V         PH   G G    + A+    +    +  Y  FL  NG+  K
Sbjct:  102 VNREYEQVFSGFSMKLPANEIPKLLAVKDVKAVYPNVTYKTDNMKDKDVTISEDAVSPQMDDSAPYIGANDAWDLGYTGKGIKVAIIDTGVEYNHPDLK-KNFGQ---------YKGYDFVDND----------YDPKETPTGD----------PRGEATDHGTHVAGTVA------------ANGTIKGVAPDATLLAYRVLGPGGS-GTTENVIAGVERAVQDGADVMNLSLGNS-LNNPDW---ATSTALDWAMSEGVVAVTSNGNSGPNGWTVGSPGTSREAISVGATQLPLNEY--------------AVTFGSYSSAKVMGYNKEDDVKALNNKEVELVEAGIGEAKDFEGKDLTGKVAVVKRG--SIAFVDKADNAKKAGAIGMVVYNNLSGEIEANVPGMS--VPTIKLSLEDGEKLVSALKAGETKTTFKLTVSKALGEQ----VADFSSRGP-VMDTWMIKPDISAPGVNIVSTIPTHDPDH-------PYGYGSKQGTSMASPHIAGAVAVIKQAKPKWSVEQIKAAIMNTAVTLKDSDGEV--------YPHNAQGAGSARIMNAIKADSLVSPGSYSYGTFLKENGNETK 619          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy Swiss-Prot
Match: P2P_LACPA (PII-type proteinase OS=Lactobacillus paracasei GN=prtP PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.462e-9
Identity = 116/476 (24.37%), Postives = 192/476 (40.34%), Query Frame = 3

Query:  813 VIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKK-SDCNRKLIGARY------YNVMHASNGNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGST----ILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDR-GFHSKVLLGN-----GRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEK----------------------VKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDS----GPFPFTEVGTTIGYKILKYINSNKNPS-AIILPTVEIPGIK-PAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVK 2105
             ++ +IDTGI    P+ KD  + +     K V +   D +K +D  +     RY      Y   +A N +  T   VD+       HG H A I       +     +      G +P  ++ A KV +      +T    ++ AI+D+ K G D++++S+G+ +  Q   L+DP      +A + G     S GN G    T  +A   +      N D  G     ++G      G     SA N   +++  T  +  GKD+      I  + + + GS D +K                       KGKI +   G   ++   K+  A+ A A GLI++N +  + P  S      FP   + +  G K++ ++ ++ + S  + +    +P  K     ++ F+S GP  ++    KPDI APG  I                    GY   SGTSMA P + G+ AL+K
Sbjct:  211 TVVSVIDTGI---DPTHKDMRLSDD----KDVKLTKYDVEKFTDTAKH---GRYFTSKVPYGFNYADNNDTITDDTVDEQ------HGMHVAGIIGANGTGDDPTKSVV-----GVAPEAQLLAMKVFTNSDTSATTGSATLVSAIEDSAKIGADVLNMSLGSDSGNQT--LEDPEIAAVQNANESGTAAVISAGNSG----TSGSATQGV------NKDYYGLQDNEMVGTPGTSRGATTVASAENTDVISQAVT--ITDGKDLQLGPETIQLSSNDFTGSFDQKKFYVVKDASGDLSKGAAADYTADAKGKIAIVKRG--ELNFADKQKYAQAAGAAGLIIVNNDGTATPLTSIRLTTTFPTFGLSSKTGQKLVDWVTAHPDDSLGVKIALTLLPNQKYTEDKMSDFTSYGP--VSNLSFKPDITAPGGNIWST-------------QNNNGYTNMSGTSMASPFIAGSQALLK 634          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy Swiss-Prot
Match: TKSP_THEKO (Subtilisin-like serine protease OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) GN=TK1689 PE=1 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.075e-9
Identity = 38/105 (36.19%), Postives = 57/105 (54.29%), Query Frame = 3

Query: 1860 VEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKP--GGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATV 2168
            + +  +    V+  FSSRGP+   +N LKP+++APG  I+ A        S +   +P    Y    GTSMA PHV G +AL+   HP WT   +++AL+ TA +
Sbjct:  321 ITVGAVDKYDVITDFSSRGPTA--DNRLKPEVVAPGNWIIAA------RASGTSMGQPINDYYTAAPGTSMATPHVAGIAALLLQAHPSWTPDKVKTALIETADI 417          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy Swiss-Prot
Match: SUBT_BACAM (Subtilisin BPN' OS=Bacillus amyloliquefaciens GN=apr PE=1 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 3.108e-9
Identity = 48/126 (38.10%), Postives = 64/126 (50.79%), Query Frame = 3

Query: 1788 TTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTAT 2165
            +T+GY         K PS I +  V+    +     A FSS GP +        D+MAPGV+I          +S   G K G Y   +GTSMA PHV GA+AL+ S HP WT++ +RS+L  T T
Sbjct:  270 STVGYP-------GKYPSVIAVGAVDSSNQR-----ASFSSVGPEL--------DVMAPGVSI----------QSTLPGNKYGAY---NGTSMASPHVAGAAALILSKHPNWTNTQVRSSLENTTT 362          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy Swiss-Prot
Match: SUBT_BACSA (Subtilisin amylosacchariticus OS=Bacillus subtilis subsp. amylosacchariticus GN=apr PE=1 SV=2)

HSP 1 Score: 63.5438 bits (153), Expect = 3.531e-9
Identity = 40/108 (37.04%), Postives = 55/108 (50.93%), Query Frame = 3

Query: 1857 TVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGG-YALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDN 2177
            T+ +  +  +   A FSS G  +        D+MAPGV+I   +              PGG Y   +GTSMA PHV GA+AL+ S HP WT++ +R  L +TAT   N
Sbjct:  280 TIAVGAVNSSNQRASFSSAGSEL--------DVMAPGVSIQSTL--------------PGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGN 365          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy Swiss-Prot
Match: SUBT_GEOSE (Subtilisin J OS=Geobacillus stearothermophilus GN=aprJ PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 3.562e-9
Identity = 40/108 (37.04%), Postives = 55/108 (50.93%), Query Frame = 3

Query: 1857 TVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGG-YALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDN 2177
            T+ +  +  +   A FSS G  +        D+MAPGV+I   +              PGG Y   +GTSMA PHV GA+AL+ S HP WT++ +R  L +TAT   N
Sbjct:  280 TIAVGAVNSSNQRASFSSAGSEL--------DVMAPGVSIQSTL--------------PGGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGN 365          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy TrEMBL
Match: A0A0D2V8Q0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G001600 PE=4 SV=1)

HSP 1 Score: 1409.05 bits (3646), Expect = 0.000e+0
Identity = 709/718 (98.75%), Postives = 711/718 (99.03%), Query Frame = 3

Query:  555 FLVTFSGREERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTKFKCPTKSSDDLXXXXXXXXXXXXKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE 2708
             L T   REERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTASIAAGAIVANASYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQP+FLKDPIAIGAFHAEQMGVMV CSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYI SNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEE+ASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTKFKCPTKSSDDLISNINYPSISISKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE
Sbjct:   81 LLSTVIPREERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTASIAAGAIVANASYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPDFLKDPIAIGAFHAEQMGVMVVCSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYIKSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEETASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTKFKCPTKSSDDLISNINYPSISISKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE 798          

HSP 2 Score: 134.035 bits (336), Expect = 8.566e-29
Identity = 86/88 (97.73%), Postives = 86/88 (97.73%), Query Frame = 2

Query:  233 MLSSQLKFPCAKKLSHSPYKYTEFILAKSTTNTITIMXXXXXXXXXXXXXXXXXXXQTPKRYVVYMGSSAGETAESDHLQLLSTVIPR 496
            MLSSQLKFPCAKKLSHSPYKYTEFILAKSTTNTITIMSCLLQLLSFFLIISSA SIQTPK YVVYMGSSAGETAESDHLQLLSTVIPR
Sbjct:    1 MLSSQLKFPCAKKLSHSPYKYTEFILAKSTTNTITIMSCLLQLLSFFLIISSAASIQTPKHYVVYMGSSAGETAESDHLQLLSTVIPR 88          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy TrEMBL
Match: A0A0D2W1B5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G001600 PE=4 SV=1)

HSP 1 Score: 1228 bits (3176), Expect = 0.000e+0
Identity = 624/628 (99.36%), Postives = 626/628 (99.68%), Query Frame = 3

Query:  825 MIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTKFKCPTKSSDDLXXXXXXXXXXXXKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE 2708
            MIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTASIAAGAIVANASYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQP+FLKDPIAIGAFHAEQMGVMV CSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYI SNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEE+ASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTKFKCPTKSSDDLISNINYPSISISKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE
Sbjct:    1 MIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTASIAAGAIVANASYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPDFLKDPIAIGAFHAEQMGVMVVCSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYIKSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEETASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTKFKCPTKSSDDLISNINYPSISISKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE 628          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy TrEMBL
Match: A0A061EVM6_THECC (Subtilisin-like serine endopeptidase family protein OS=Theobroma cacao GN=TCM_024130 PE=4 SV=1)

HSP 1 Score: 1122.84 bits (2903), Expect = 0.000e+0
Identity = 567/718 (78.97%), Postives = 630/718 (87.74%), Query Frame = 3

Query:  555 FLVTFSGREERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTKFKCPTKSSDDLXXXXXXXXXXXXKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE 2708
             L T   R++  RIS+ +HYNHALRGFSAMLT++EASEL+G +GVVS+FPDS+LQLHTTRSWDFL+  S P+ +HGSY  HKS+YDVIIGMID GIW ESPSF+DEGMGEIP RWKGVCMEGPDFKKS+CNRKLIGARYYNV+H SNGN TTQMRVDKSPRDTVGHGTHTAS AAGA V NASY+GLAQGTARGGSP  RIA YK CSEDGCP ST LKAIDDA+KDGVDIISISIG S+ LQ ++LKDPIAIGAFHAEQ+GVMV CSGGNEGPDPFT+ NAAPWIFTVAASNIDR F S VLLGNGR FQGSAINFSNLTR+ETYPLAYGKDIAAK+SPI EARSCYPGSLDPE+VKGK+I+C D FP+VSR IKKLVAEDA+AKGLILINEN + APFDSG FPFTEVG+  GYKILKYINSNKNP+A ILPT ++P  +PAPVVAYFSSRGPSVL ENILKPDIMAPGVAIL A+ PK        G KP  YA++SGTSMACPH+TGASA +KSVHPKWTSSMIRSALMTTATVYDNM KP+TN S SFATPHE GVGEISP+KALNPGLVFETT+EDYL+FLCY+GS EKTIRSM+KT FKCP KSSD+LIS+INYPSISIS+L+K+ GF TI RSVTNVG  NVTYT+T QAP+G+KVKV+PK ITF+ENV+RV F+V FDG  AS+GYNFGS+TWS   YSVR VFAVNV+
Sbjct:   51 LLSTVIPRQDSGRISLIHHYNHALRGFSAMLTENEASELAGHDGVVSLFPDSVLQLHTTRSWDFLEGQSRPRFSHGSY-QHKSSYDVIIGMIDGGIWPESPSFRDEGMGEIPTRWKGVCMEGPDFKKSNCNRKLIGARYYNVLHTSNGNKTTQMRVDKSPRDTVGHGTHTASTAAGAQVDNASYNGLAQGTARGGSPNARIAMYKACSEDGCPSSTTLKAIDDAIKDGVDIISISIGMSSLLQSDYLKDPIAIGAFHAEQLGVMVVCSGGNEGPDPFTIINAAPWIFTVAASNIDRDFQSTVLLGNGRTFQGSAINFSNLTRSETYPLAYGKDIAAKFSPISEARSCYPGSLDPERVKGKVIICVDSFPIVSREIKKLVAEDAQAKGLILINENGKGAPFDSGAFPFTEVGSATGYKILKYINSNKNPTATILPTADVPRHRPAPVVAYFSSRGPSVLAENILKPDIMAPGVAILAAVIPK-----VVQGKKPLEYAIKSGTSMACPHITGASAFIKSVHPKWTSSMIRSALMTTATVYDNMGKPLTNSSGSFATPHETGVGEISPLKALNPGLVFETTSEDYLKFLCYSGSSEKTIRSMAKTNFKCPRKSSDNLISSINYPSISISRLDKTRGFRTITRSVTNVGLLNVTYTATAQAPLGLKVKVLPKTITFVENVRRVPFRVSFDGRGASTGYNFGSLTWSGGPYSVRMVFAVNVK 762          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy TrEMBL
Match: M5X7H3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001918mg PE=4 SV=1)

HSP 1 Score: 947.577 bits (2448), Expect = 0.000e+0
Identity = 487/710 (68.59%), Postives = 574/710 (80.85%), Query Frame = 3

Query:  579 EERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTKFKCPTKSSDDLXXXXXXXXXXXXKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE 2708
             E ER+SI + YNHA RGFSAMLT+ EAS LSG + VVS+FPDSIL+LHTTRSWDFL+  S  +L    Y    S+ DVIIGMIDTGIW ES SF DEG+G +P RWKGVCMEG DF+KS+CNRKLIGARYYNV    +GN ++  R   SPRD+VGHGTHTAS AAG  V NASY+GLAQGTARGG P  RIA YK CS+ GC G+TILKAIDDA++DGVDIISISIG S+  Q ++L DPIAIGAFHAEQMGVMV CSGGN+GPDP+T+ N APWIFTVAASNIDR F S ++LGNG+ F GSAINFSNLTR+ TYPL +GKD+A  Y+P+ EAR+CYPGSLDP+KV GKI+VC D  P VSR IKKLV EDAKAKGLILI+E ++S PFDSG FP+TEVG   G++IL+YINS KNP+A ILPTV++P  +PAP VAYFSSRGP+ LTENILKPDIMAPGVAIL AI PK+E  +  +G KP  ++++SGTSMACPHVTGA+A +KSVH +WTSSMI+SALMTTATV++NM+KP+TN S +FA PHE+GVGEI+P+KAL+PGLVFETTTE+YL FLCY G PEK IRSMS TKF CP  S D+LISN+NYPSISISKL +     TI+R+ TNV   N TY + V AP G+ VKV+P+K+ F E V+RVSF+V F G EA  GYNFGSITW   ++SVRTVF+VNVE
Sbjct:   34 HEIERLSIIHKYNHAFRGFSAMLTETEASVLSGHDDVVSIFPDSILELHTTRSWDFLEAESG-RLPSNKYQRGLSS-DVIIGMIDTGIWPESSSFNDEGIGAVPSRWKGVCMEGSDFRKSNCNRKLIGARYYNVPWTRDGNQSSLARTKGSPRDSVGHGTHTASTAAGVQVLNASYYGLAQGTARGGLPSARIACYKACSDVGCSGATILKAIDDAIRDGVDIISISIGMSSLFQSDYLNDPIAIGAFHAEQMGVMVICSGGNDGPDPYTIVNTAPWIFTVAASNIDRDFQSNIVLGNGKNFTGSAINFSNLTRSRTYPLVFGKDVAGYYTPVSEARNCYPGSLDPKKVVGKIVVCVDDDPAVSRKIKKLVVEDAKAKGLILIDEAEKSVPFDSGIFPYTEVGNIAGFQILQYINSTKNPTATILPTVDVPRYRPAPAVAYFSSRGPAELTENILKPDIMAPGVAILAAIAPKNETGTVPNGKKPSTFSIKSGTSMACPHVTGAAAFIKSVHRRWTSSMIKSALMTTATVFNNMKKPLTNSSNTFANPHEVGVGEINPLKALSPGLVFETTTENYLEFLCYYGYPEKNIRSMSNTKFICPKSSIDELISNVNYPSISISKLNRHQPAKTIQRTATNVAALNSTYIAKVHAPAGLIVKVLPEKLVFAEGVRRVSFQVSFYGKEAPRGYNFGSITWFDGRHSVRTVFSVNVE 741          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy TrEMBL
Match: B9SHW1_RICCO (Xylem serine proteinase 1, putative OS=Ricinus communis GN=RCOM_0622480 PE=4 SV=1)

HSP 1 Score: 921.768 bits (2381), Expect = 0.000e+0
Identity = 475/711 (66.81%), Postives = 562/711 (79.04%), Query Frame = 3

Query:  579 EERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQM-RVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTKFKCPTKSSDDLXXXXXXXXXXXXKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE 2708
             E ERIS+ +HY+HA  GFSAMLT+ EASELSG   VVSVF D  L+LHTTRSWDFL+ NS   +       H S+ DVIIG+IDTGIW ESPSF D+G+GEIP RWKGVCMEG DFKKS+CNRKLIGARYY+ +  +  NN T + + + SPRD +GHGTHTASIA GA VAN SY+GLA+GTARGGSP  R+A YK C+ DGC GSTIL+AIDDA+KDGVD+ISISIG S+  Q ++L DPIAIGAFHA+QMGVM+ CS GN+GPDP+T+ N+APWIFTVAASNIDR F S ++LGNG+ F+GSAINFSNL R+ TYPLA+G + AA ++P+ EAR+CYPGSLD  KV GKI+VC D  P + R IKKLV EDA+AKGLILINE +E  PFDSG FPF EVG   G ++LKYINS K P+A ILP V++P  +PAPVVAYFSSRGP+ LTENILKPDIMAPGVAIL AITPK+E  S   G KP GYA+ SGTSMACPHVTGA+A +KSVH  W+SS IRSALMTTA +Y+NM KP+TN S+S++ PHEMGVGEI+P+ AL+PGLVFETTTEDYL+FLCY G  EK IRSMS T F CP  S D LISNINYPS+SISKL++     T+KR VTNVG PN TY +T+QAP G++VKV PKK+ F E V R SFK+ F+G  A+ GYN+GS+TW    +SVR  FAV VE
Sbjct:   70 HESERISLVHHYSHAFTGFSAMLTEIEASELSGHERVVSVFKDPTLKLHTTRSWDFLEANSG--MQSSQKYSHLSS-DVIIGVIDTGIWPESPSFSDKGLGEIPSRWKGVCMEGHDFKKSNCNRKLIGARYYDTILRTYKNNKTHVAKPNGSPRDDIGHGTHTASIAGGAEVANVSYYGLARGTARGGSPSSRLAIYKACTTDGCAGSTILQAIDDAIKDGVDVISISIGLSSIFQSDYLNDPIAIGAFHAQQMGVMIICSAGNDGPDPYTIVNSAPWIFTVAASNIDRDFQSTMILGNGKTFRGSAINFSNLKRSRTYPLAFGGNAAANFTPVSEARNCYPGSLDRAKVAGKIVVCIDNDPSIPRRIKKLVVEDARAKGLILINEVEEGVPFDSGVFPFAEVGNIAGTQLLKYINSTKKPTATILPAVDVPRYRPAPVVAYFSSRGPAQLTENILKPDIMAPGVAILAAITPKNESGSVPVGKKPAGYAIRSGTSMACPHVTGAAAFIKSVHQGWSSSRIRSALMTTANIYNNMGKPLTNSSSSYSNPHEMGVGEINPLSALDPGLVFETTTEDYLQFLCYYGYSEKNIRSMSNTNFNCPRVSFDKLISNINYPSVSISKLDRHQPARTVKRIVTNVGSPNSTYVTTLQAPQGLEVKVTPKKLIFKEGVSRKSFKISFNGKMATKGYNYGSVTWVDGTHSVRLTFAVYVE 777          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy TrEMBL
Match: A0A0D2TSD9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G001600 PE=4 SV=1)

HSP 1 Score: 921.383 bits (2380), Expect = 0.000e+0
Identity = 456/464 (98.28%), Postives = 457/464 (98.49%), Query Frame = 3

Query:  555 FLVTFSGREERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILK 1946
             L T   REERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTASIAAGAIVANASYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQP+FLKDPIAIGAFHAEQMGVMV CSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYI SNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILK
Sbjct:   81 LLSTVIPREERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTASIAAGAIVANASYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPDFLKDPIAIGAFHAEQMGVMVVCSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYIKSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILK 544          

HSP 2 Score: 134.035 bits (336), Expect = 1.969e-29
Identity = 86/88 (97.73%), Postives = 86/88 (97.73%), Query Frame = 2

Query:  233 MLSSQLKFPCAKKLSHSPYKYTEFILAKSTTNTITIMXXXXXXXXXXXXXXXXXXXQTPKRYVVYMGSSAGETAESDHLQLLSTVIPR 496
            MLSSQLKFPCAKKLSHSPYKYTEFILAKSTTNTITIMSCLLQLLSFFLIISSA SIQTPK YVVYMGSSAGETAESDHLQLLSTVIPR
Sbjct:    1 MLSSQLKFPCAKKLSHSPYKYTEFILAKSTTNTITIMSCLLQLLSFFLIISSAASIQTPKHYVVYMGSSAGETAESDHLQLLSTVIPR 88          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy TrEMBL
Match: A0A067L7X2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24599 PE=4 SV=1)

HSP 1 Score: 921.383 bits (2380), Expect = 0.000e+0
Identity = 486/752 (64.63%), Postives = 581/752 (77.26%), Query Frame = 3

Query:  471 NYCPQ*FQGFIT*SITH----DRSFIHSSTEIFLVTFSGREERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQM-RVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTKFKCPTKSSDDLXXXXXXXXXXXXKLEK-SIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE 2708
            N  PQ +  ++  SI++    D  F  S+    L +    +E ERIS+ +HYNHA  GFSAMLT+ EASELS    V+SVF D IL+LHTTRSWDFL+  S  +++H     H S+ DVIIGMIDTGIW ESPSF D+G+GEIP RWKG+CMEG DFKKS+CNRKLIGARYY+    +  NN T + +   SPRD +GHGTHT SIA G+ VAN SY+GLA GTARGGSP  R+A YK CS DGC GS ILKA++DA+KDGVDIISISIG S+  Q ++L DPIAIGAFHA+QMGVMV CS GN+GPDPFT+ N+APWIFTVAASNIDR F S VLLGNG+ FQGSAINFSNLTR+ TYPLA+G++ AAK++P+ EA +C PGSLD EKV GKI+VC D    V R IKKLV EDA AKG++LI+E+++  PFDSG FPF+E+G+    ++LKYINS KNP+A ILP V++P  KPAPVVAYFSSRGP  LTENILKPDIMAPGVAIL A+ PK+E  S   G KP GYA+ SGTSMACPHVTGA+A +KSVH  WT SMI+SALMTTAT+Y+NM KP+TN S S++ PHEMGVGEI+P+KAL+PGL+FETT EDYL+FLCY G  EK IR M+ TKF CP  S D LISNINYPSISISKL++     +TIKRSV NVG PN TY + V+AP G+ VKV+PKK+ F E + R+SFKV FDG  AS+GYNFGS+TW   ++SVR VF VNVE
Sbjct:   27 NQIPQKYVVYMGSSISNGNEGDAKFAESAHLQLLTSIIPSQESERISLIHHYNHAFNGFSAMLTESEASELSENEEVISVFKDPILKLHTTRSWDFLEAKSGMQISH--QYSHLSS-DVIIGMIDTGIWPESPSFDDKGIGEIPSRWKGICMEGHDFKKSNCNRKLIGARYYDTYQRTYKNNKTHVPKPSGSPRDYIGHGTHTTSIAGGSEVANVSYYGLAAGTARGGSPSTRLAIYKACSLDGCSGSIILKAMEDAIKDGVDIISISIGMSSIFQSDYLNDPIAIGAFHAQQMGVMVICSAGNDGPDPFTIVNSAPWIFTVAASNIDRDFQSTVLLGNGKTFQGSAINFSNLTRSRTYPLAFGEEAAAKFTPVSEASNCDPGSLDREKVAGKILVCTDSEFSVPRQIKKLVVEDAGAKGMLLIHEDEKGVPFDSGVFPFSELGSFAASQLLKYINSTKNPTATILPAVDVPRYKPAPVVAYFSSRGPGQLTENILKPDIMAPGVAILAAMIPKNESGSVPIGKKPSGYAIRSGTSMACPHVTGAAAFIKSVHRGWTPSMIKSALMTTATIYNNMGKPITNSSHSYSNPHEMGVGEINPLKALHPGLIFETTMEDYLQFLCYYGYSEKNIRLMANTKFNCPRISFDKLISNINYPSISISKLDRHQAAQITIKRSVINVGTPNATYIAKVEAPQGLLVKVLPKKLVFKEGLSRLSFKVSFDGQMASNGYNFGSVTWLDGRHSVRIVFTVNVE 775          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy TrEMBL
Match: V4T314_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000349mg PE=4 SV=1)

HSP 1 Score: 919.072 bits (2374), Expect = 0.000e+0
Identity = 483/715 (67.55%), Postives = 570/715 (79.72%), Query Frame = 3

Query:  579 EERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKS---TYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDES-APFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSK-TKFKCPTKSSDDLXXXXXXXXXXXXKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE 2708
            EE +RIS+ +HY H+ +GFSAMLT+ EAS LSG   +VSVFPD IL+LHTTRSWDFL+  +  K    ++  HK    + DVIIG+IDTGIW ESPSFKD GM EIP +WKGVCM+  DFKKS+CNRKLIGAR+Y++   SN +NTT+  +  SPRD+VGHGTHTAS AAGA VANASY GLA+GTARGGSP  RIA+YK CSEDGC GS IL+A+DDA+ DGVDIISISIG S+  Q ++L DPIAIGAFHAEQMGVMV CS GN+GPDP TV N APWIFTV AS+IDR F S VLLGNG+  +GSAI+ SNL+ + TYP+A+GKDIAAK++P+ EAR+C PGSLDP+KV GKIIVC D  P V R IKKLVAEDA AKGLILI+E+ E   PFDSG FPF+EVG+  G++I+ YINS KNP+A ILPTV++PG KPAPVVAYFSSRGP  LTENILKPD+ APGVAIL AI PK    S   G KP GYA+ SGTSMACPHVTGA+A ++SVH +W+SS+I+SALMTTATVYDN  KP+ N + S A+PHE GVGEISP+KALNPGLVFETTT+DYLRFLCY G  EK IRSMSK T F CP  S D+LISNINYPSISISKL++     T+KR+VTNVG  NVTY S V AP G+ VKV+P+K+ F E VKR+SF V F G EA+ GYNFGS+TWS  ++SV+ +FAVNV+
Sbjct:   65 EESDRISLIHHYKHSFKGFSAMLTEKEASVLSGHEKIVSVFPDPILKLHTTRSWDFLEAEAEAKAPTSTWSSHKYHNISSDVIIGIIDTGIWPESPSFKDRGMSEIPSKWKGVCMDSHDFKKSNCNRKLIGARFYSIPLTSNNHNTTRTTLAGSPRDSVGHGTHTASTAAGAHVANASYFGLARGTARGGSPSSRIASYKACSEDGCSGSAILQAMDDAIADGVDIISISIGMSSLFQSDYLNDPIAIGAFHAEQMGVMVICSAGNDGPDPSTVVNTAPWIFTVGASSIDRDFQSTVLLGNGKTIKGSAISLSNLSSSMTYPIAFGKDIAAKFTPVSEARTCRPGSLDPKKVAGKIIVCVDDDPTVPRKIKKLVAEDADAKGLILIDEDYEKHVPFDSGIFPFSEVGSVAGFQIIHYINSTKNPTATILPTVDVPGYKPAPVVAYFSSRGPGELTENILKPDVTAPGVAILAAIVPKANAGSFPIGKKPAGYAITSGTSMACPHVTGAAAFIRSVHRRWSSSIIKSALMTTATVYDNTGKPLKNNAGSTASPHETGVGEISPLKALNPGLVFETTTKDYLRFLCYFGYSEKNIRSMSKYTNFNCPRNSIDNLISNINYPSISISKLDRHRAAETVKRTVTNVGLQNVTYISRVNAPSGLIVKVLPQKLVFAEGVKRMSFSVSFYGKEAAGGYNFGSVTWSDNRHSVQMMFAVNVQ 779          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy TrEMBL
Match: V4T887_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000349mg PE=4 SV=1)

HSP 1 Score: 914.835 bits (2363), Expect = 0.000e+0
Identity = 483/716 (67.46%), Postives = 570/716 (79.61%), Query Frame = 3

Query:  579 EERERISITNHYNHALRGFSAMLTDDEASELS-GRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKS---TYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHASNGNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDES-APFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSK-TKFKCPTKSSDDLXXXXXXXXXXXXKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE 2708
            EE +RIS+ +HY H+ +GFSAMLT+ EAS LS G   +VSVFPD IL+LHTTRSWDFL+  +  K    ++  HK    + DVIIG+IDTGIW ESPSFKD GM EIP +WKGVCM+  DFKKS+CNRKLIGAR+Y++   SN +NTT+  +  SPRD+VGHGTHTAS AAGA VANASY GLA+GTARGGSP  RIA+YK CSEDGC GS IL+A+DDA+ DGVDIISISIG S+  Q ++L DPIAIGAFHAEQMGVMV CS GN+GPDP TV N APWIFTV AS+IDR F S VLLGNG+  +GSAI+ SNL+ + TYP+A+GKDIAAK++P+ EAR+C PGSLDP+KV GKIIVC D  P V R IKKLVAEDA AKGLILI+E+ E   PFDSG FPF+EVG+  G++I+ YINS KNP+A ILPTV++PG KPAPVVAYFSSRGP  LTENILKPD+ APGVAIL AI PK    S   G KP GYA+ SGTSMACPHVTGA+A ++SVH +W+SS+I+SALMTTATVYDN  KP+ N + S A+PHE GVGEISP+KALNPGLVFETTT+DYLRFLCY G  EK IRSMSK T F CP  S D+LISNINYPSISISKL++     T+KR+VTNVG  NVTY S V AP G+ VKV+P+K+ F E VKR+SF V F G EA+ GYNFGS+TWS  ++SV+ +FAVNV+
Sbjct:   65 EESDRISLIHHYKHSFKGFSAMLTEKEASVLSAGHEKIVSVFPDPILKLHTTRSWDFLEAEAEAKAPTSTWSSHKYHNISSDVIIGIIDTGIWPESPSFKDRGMSEIPSKWKGVCMDSHDFKKSNCNRKLIGARFYSIPLTSNNHNTTRTTLAGSPRDSVGHGTHTASTAAGAHVANASYFGLARGTARGGSPSSRIASYKACSEDGCSGSAILQAMDDAIADGVDIISISIGMSSLFQSDYLNDPIAIGAFHAEQMGVMVICSAGNDGPDPSTVVNTAPWIFTVGASSIDRDFQSTVLLGNGKTIKGSAISLSNLSSSMTYPIAFGKDIAAKFTPVSEARTCRPGSLDPKKVAGKIIVCVDDDPTVPRKIKKLVAEDADAKGLILIDEDYEKHVPFDSGIFPFSEVGSVAGFQIIHYINSTKNPTATILPTVDVPGYKPAPVVAYFSSRGPGELTENILKPDVTAPGVAILAAIVPKANAGSFPIGKKPAGYAITSGTSMACPHVTGAAAFIRSVHRRWSSSIIKSALMTTATVYDNTGKPLKNNAGSTASPHETGVGEISPLKALNPGLVFETTTKDYLRFLCYFGYSEKNIRSMSKYTNFNCPRNSIDNLISNINYPSISISKLDRHRAAETVKRTVTNVGLQNVTYISRVNAPSGLIVKVLPQKLVFAEGVKRMSFSVSFYGKEAAGGYNFGSVTWSDNRHSVQMMFAVNVQ 780          

BLAST of g.barbadense_cottongen_reftransV1_0097827 vs. ExPASy TrEMBL
Match: F6HIW6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0047g00200 PE=4 SV=1)

HSP 1 Score: 913.294 bits (2359), Expect = 0.000e+0
Identity = 470/714 (65.83%), Postives = 560/714 (78.43%), Query Frame = 3

Query:  582 ERERISITNHYNHALRGFSAMLTDDEASELSGRNGVVSVFPDSILQLHTTRSWDFLDDNSNPKLNHGSYLPHKSTYDVIIGMIDTGIWAESPSFKDEGMGEIPKRWKGVCMEGPDFKKSDCNRKLIGARYYNVMHA-----SNGNNTTQMRVDKSPRDTVGHGTHTXXXXXXXXXXXXXYHGLAQGTARGGSPFFRIAAYKVCSEDGCPGSTILKAIDDAVKDGVDIISISIGTSAFLQPNFLKDPIAIGAFHAEQMGVMVACSGGNEGPDPFTVTNAAPWIFTVAASNIDRGFHSKVLLGNGRIFQGSAINFSNLTRTETYPLAYGKDIAAKYSPIPEARSCYPGSLDPEKVKGKIIVCFDGFPVVSRTIKKLVAEDAKAKGLILINENDESAPFDSGPFPFTEVGTTIGYKILKYINSNKNPSAIILPTVEIPGIKPAPVVAYFSSRGPSVLTENILKPDIMAPGVAILGAITPKDEEESASDGVKPGGYALESGTSMACPHVTGASALVKSVHPKWTSSMIRSALMTTATVYDNMRKPVTNGSASFATPHEMGVGEISPVKALNPGLVFETTTEDYLRFLCYNGSPEKTIRSMSKTKFKCPTKSSDDLXXXXXXXXXXXXKLEKSIGFLTIKRSVTNVGHPNVTYTSTVQAPMGMKVKVIPKKITFLENVKRVSFKVLFDGSEASSGYNFGSITWSAAQYSVRTVFAVNVE 2708
            E ERIS+ + YNHA +GFSAMLT  EAS LSG   +VS+FPD +LQLHTTRSWDFL+  S   +       H  + DVIIG+IDTGIW ESPSF D G+GEIP RWKGVCMEG DFKKS+CNRKLIGARYYN   A     S+ N +  + +  SPRD+VGHGTHTASIAAGA +ANASY+GLA GTARGGSP  RIA+YK CS +GC GSTI+KA DDA+KDGVDIIS+SIG ++  Q +FL DPIAIGAFHA+QMGVMV CS GN GPDP+T+ N+APWIFTVAASNIDR F S V+LGNG+ F G AINFSNLTR++TYPLA  +D+AA ++P  +ARSCYPGSLDP+KV+GKIIVC  G     R I+KLV EDAKA G+ILI+E  + +PF+SG +PFTEVG   G+ ILKYINS KNP+A ILPT E+P I+PAPVVA+FSSRGP  LTENILKPDIMAPGVAIL A+ PK E  S   G K   + + SGTSMACPHVTGA+A +KSVHP+W+SSMIRSALMTTA + +NMRK +TN +   A PHEMGVGEISP++ALNPGLVFET +EDYL FLCY G PEKTIR+++  KF CP+ S D+LISNINYPSISISKL++ +   T+ R+V NVG PN TY + + AP+G+++ V PKKI F+E ++R +FKV F G EAS GY+FGSITW    +SVRTVFAVNVE
Sbjct:   31 ESERISLIHSYNHAFKGFSAMLTQGEASILSGHEEIVSIFPDPLLQLHTTRSWDFLNVESG--ITSTPLFHHNLSRDVIIGVIDTGIWPESPSFSDNGIGEIPSRWKGVCMEGSDFKKSNCNRKLIGARYYNTPKALIQPKSSSNKSHPINLTGSPRDSVGHGTHTASIAAGAPIANASYYGLAPGTARGGSPSARIASYKACSLEGCSGSTIMKAFDDAIKDGVDIISVSIGMTSIFQSDFLNDPIAIGAFHAQQMGVMVVCSAGNSGPDPYTIVNSAPWIFTVAASNIDRDFQSTVVLGNGKTFPGPAINFSNLTRSKTYPLARSEDVAAAFTPSSDARSCYPGSLDPKKVRGKIIVC-SGDGSNPRRIQKLVVEDAKAIGMILIDEYQKGSPFESGIYPFTEVGDIAGFHILKYINSTKNPTATILPTKEVPRIRPAPVVAFFSSRGPGGLTENILKPDIMAPGVAILAAMIPKTEVGSVPIGRKVSKFGIRSGTSMACPHVTGAAAFIKSVHPQWSSSMIRSALMTTAIISNNMRKDLTNSTGFSANPHEMGVGEISPLRALNPGLVFETASEDYLHFLCYYGYPEKTIRAVANKKFTCPSTSFDELISNINYPSISISKLDRHLAAQTVTRTVRNVGSPNSTYIAQLHAPVGLEITVSPKKIVFVEGLERATFKVSFKGKEASRGYSFGSITWFDGLHSVRTVFAVNVE 741