g.barbadense_cottongen_reftransV1_0097431, g.barbadense_cottongen_reftransV1_0097431 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy Swiss-Prot
Match: PP395_ARATH (Pentatricopeptide repeat-containing protein At5g21222 OS=Arabidopsis thaliana GN=ATC401 PE=2 SV=1)

HSP 1 Score: 668.692 bits (1724), Expect = 0.000e+0
Identity = 307/456 (67.32%), Postives = 381/456 (83.55%), Query Frame = -2

Query:  725 SSKCIVCSGNNSCQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIF-EMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEI-GVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLN 2086
            S  C++CSG  +C  VR+RTKLMN LIE+G+PQEA  IFN+L EEGHKP+L+TYT LV+ALTR K + S++SLISKVE+NG+KPD+ILFNA+INA SESGN+ +AMKIF+KMK+SGCKPT STFNTL+KGYG IGK EESS+LL++ML+D  +QPNDRT NIL++AWCN+  IEEAWN+V++M + G+ PDVVT+NT+A+AYA+ G T  AE MI   M +NKV PNVRTCG IV GYC+EG M EALRF YRMKELGV PNL VFNSL+KGFL++ D +GV E + LMEEFGVKPDV+TFST+MNAWSS G M +C+EI+ DM++ GI+PD+HAFSILAKGY RAGEP +AE +LN M KFGV+PNVVI+T +ISGWC+AG+M  A +VY+KMC I G++PNL TYETLIWG+GEAKQPWKAEELL+ ME K +   R T+QL+AD W++IG+ N
Sbjct:  304 SFPCVICSGGTTCGDVRSRTKLMNGLIERGRPQEAHSIFNTLIEEGHKPSLITYTTLVTALTRQKHFHSLLSLISKVEKNGLKPDTILFNAIINASSESGNLDQAMKIFEKMKESGCKPTASTFNTLIKGYGKIGKLEESSRLLDMMLRDEMLQPNDRTCNILVQAWCNQRKIEEAWNIVYKMQSYGVKPDVVTFNTLAKAYARIGSTCTAEDMIIPRMLHNKVKPNVRTCGTIVNGYCEEGKMEEALRFFYRMKELGVHPNLFVFNSLIKGFLNINDMDGVGEVVDLMEEFGVKPDVVTFSTLMNAWSSVGDMKRCEEIYTDMLEGGIDPDIHAFSILAKGYARAGEPEKAEQILNQMRKFGVRPNVVIYTQIISGWCSAGEMKKAMQVYKKMCGIVGLSPNLTTYETLIWGFGEAKQPWKAEELLKDMEGKNVVPTRKTMQLIADGWKSIGVSN 759          

HSP 2 Score: 194.126 bits (492), Expect = 3.029e-50
Identity = 105/376 (27.93%), Postives = 196/376 (52.13%), Query Frame = -2

Query:  698 MINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELM--LQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMI-FEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDT-NGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNV 1813
            ++N   E G  +EA  IF  + + G KP+  T+ TL+     + + +    LL L+  ++   ++P+   +N +I A     N+++A  +  +M  SG  P   T+NT+ + Y + G+   + +++   +++  + PN RTC I+V  +C +  + EA   VY+M+  GV+P++V FN+L K +  +  T    D  +  M    VKP+V T  TI+N +   G M++    F  M + G+ P++  F+ L KG++   +      +++ M +FGV+P+VV F+T+++ W + G M     +Y  M E G+ P++  +  L  GY  A +P KAE++L  M + G+         +   W + G + +A ++ K +
Sbjct:  325 LMNGLIERGRPQEAHSIFNTLIEEGHKPSLITYTTLVTA---LTRQKHFHSLLSLISKVEKNGLKPDTILFNAIINASSESGNLDQAMKIFEKMKESGCKPTASTFNTLIKGYGKIGKLEESSRLLDMMLRDEMLQPNDRTCNILVQAWCNQRKIEEAWNIVYKMQSYGVKPDVVTFNTLAKAYARIGSTCTAEDMIIPRMLHNKVKPNVRTCGTIVNGYCEEGKMEEALRFFYRMKELGVHPNLFVFNSLIKGFLNINDMDGVGEVVDLMEEFGVKPDVVTFSTLMNAWSSVGDMKRCEEIYTDMLEGGIDPDIHAFSILAKGYARAGEPEKAEQILNQMRKFGVRPNVVIYTQIISGWCSAGEMKKAMQVYKKM 697          

HSP 3 Score: 141.354 bits (355), Expect = 4.678e-33
Identity = 85/318 (26.73%), Postives = 158/318 (49.69%), Query Frame = -2

Query:  689 GNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLM-EEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLL-NSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNVDRG 1636
            G+V+   +  N LI     +   +EA ++ + ++  G  P ++TY T+  A  +    +    +I +++ N + P+      I+    + GN+ +A++   +MKE G +P    FN+L+KG+  +         L +M  +  ++P+  T + ++ AW +   +++   I   M   G++PDV  F+ LAK Y R G    AE ++   M    V+PNV    T+++G+C  GKM  A R + +M E+GV PNL  + +LI G+          E++ +MEE G+  +  T   + +AW ++G +     I  ++  G
Sbjct:  317 GDVRSRTKLMNGLIE----RGRPQEAHSIFNTLIEEGHKPSLITYTTLVTALTRQKHFHSLLSLISKVEKNGLKPDTILFNAIINASSESGNLDQAMKIFEKMKESGCKPTASTFNTLIKGYGKIGKLEESSRLLDMMLRDEMLQPNDRTCNILVQAWCNQRKIEEAWNIVYKMQSYGVKPDVVTFNTLAKAYARIGSTCTAEDMIIPRMLHNKVKPNVRTCGTIVNGYCEEGKMEEALRFFYRMKELGVHPNLFVFNSLIKGFLNINDMDGVGEVVDLMEEFGVKPDVVTFSTLMNAWSSVGDMKRCEEIYTDMLEG 630          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy Swiss-Prot
Match: PP397_ARATH (Pentatricopeptide repeat-containing protein At5g25630 OS=Arabidopsis thaliana GN=At5g25630 PE=2 SV=2)

HSP 1 Score: 596.66 bits (1537), Expect = 0.000e+0
Identity = 297/572 (51.92%), Postives = 403/572 (70.45%), Query Frame = -2

Query:  374 CIVCSGNNSCQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQ--PNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFE--MQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNVDRGIASNTKDDIPAEGMEKTYKKQSLSASYSNVLQMPGVAMNEQNGSSSSKARSQMVLKKYDSSSLFPAQASVFGVQPGIICQKQLTTTREYRMHGQFVNSCKLVFIN 2077
            C  C   +SC+TVR+RTKLMN+LIE+G+P EAQ +F +L E GH+P+L++YT L++A+T  K+Y SI S++S+VE++G K DSI FNA+INAFSESGN+++A++   KMK+ G  PTTST+NTL+KGYG  GK E SS+LL+LML++GNV   PN RT+N+L++AWC K  +EEAW VV +M   G+ PD VTYNTIA  Y Q GET RAE  + E  +   K  PN RTCGI+V GYC+EG + + LRFV RMKE+ V+ NLVVFNSL+ GF++V D +G+DE LTLM+E  VK DVIT+ST+MNAWSSAG M+K  ++F +MVKAG++PD HA+SILAKGY+RA EP +AE LL ++     +PNVVIFTTVISGWC+ G M+ A RV+ KMC+ GV+PN+ T+ETL+WGY E KQPWKAEE+LQ+M   G+  E ST  L+A+AWR  GL +E+       ++ I +    DI    +EK Y+KQS  +S+ N+LQ+P                  +  ++  ++      A   G +  IICQKQ  +  ++ + GQFV+SC  VF++
Sbjct:   33 CKSCVEGSSCRTVRSRTKLMNVLIERGRPHEAQTVFKTLAETGHRPSLISYTTLLAAMTVQKQYGSISSIVSEVEQSGTKLDSIFFNAVINAFSESGNMEDAVQALLKMKELGLNPTTSTYNTLIKGYGIAGKPERSSELLDLMLEEGNVDVGPNIRTFNVLVQAWCKKKKVEEAWEVVKKMEECGVRPDTVTYNTIATCYVQKGETVRAESEVVEKMVMKEKAKPNGRTCGIVVGGYCREGRVRDGLRFVRRMKEMRVEANLVVFNSLINGFVEVMDRDGIDEVLTLMKECNVKADVITYSTVMNAWSSAGYMEKAAQVFKEMVKAGVKPDAHAYSILAKGYVRAKEPKKAEELLETL-IVESRPNVVIFTTVISGWCSNGSMDDAMRVFNKMCKFGVSPNIKTFETLMWGYLEVKQPWKAEEVLQMMRGCGVKPENSTFLLLAEAWRVAGLTDES-------NKAINALKCKDIEIAKLEKLYQKQSSGSSF-NLLQIP------------------VGKRELPTAKAMNLSACKLGARVPIICQKQ--SQAQFGISGQFVHSCT-VFLS 574          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy Swiss-Prot
Match: PPR12_ARATH (Pentatricopeptide repeat-containing protein At1g05670, mitochondrial OS=Arabidopsis thaliana GN=At1g05670 PE=2 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 7.937e-66
Identity = 131/462 (28.35%), Postives = 239/462 (51.73%), Query Frame = -2

Query:  677 CQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEF---GVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNVDR-GIASN 2050
            C  V +   +++ + + G+ +EA  +   +  +G+ P +++Y+ +V+   R      +  LI  ++  G+KP+S ++ ++I        + EA + F +M + G  P T  + TL+ G+   G    +SK    M    ++ P+  TY  +I  +C   ++ EA  + H M   G+ PD VT+  +   Y + G    A ++   M     +PNV T   ++ G CKEG++  A   ++ M ++G+QPN+  +NS+V G   +  +  ++EA+ L+ EF   G+  D +T++T+M+A+  +G MDK QEI  +M+  G++P +  F++L  G+   G   + E LLN M   G+ PN   F +++  +C    +  A  +Y+ MC  GV P+  TYE L+ G+ +A+   +A  L Q M+ KG S   ST  ++   +       EAR +   + R G+A++
Sbjct:  243 CWNVASYNIVIHFVCQLGRIKEAHHLLLLMELKGYTPDVISYSTVVNGYCRFGELDKVWKLIEVMKRKGLKPNSYIYGSIIGLLCRICKLAEAEEAFSEMIRQGILPDTVVYTTLIDGFCKRGDIRAASKFFYEM-HSRDITPDVLTYTAIISGFCQIGDMVEAGKLFHEMFCKGLEPDSVTFTELINGYCKAGHMKDAFRVHNHMIQAGCSPNVVTYTTLIDGLCKEGDLDSANELLHEMWKIGLQPNIFTYNSIVNG---LCKSGNIEEAVKLVGEFEAAGLNADTVTYTTLMDAYCKSGEMDKAQEILKEMLGKGLQPTIVTFNVLMNGFCLHGMLEDGEKLLNWMLAKGIAPNATTFNSLVKQYCIRNNLKAATAIYKDMCSRGVGPDGKTYENLVKGHCKARNMKEAWFLFQEMKGKGFSVSVSTYSVLIKGFLKRKKFLEAREVFDQMRREGLAAD 700          

HSP 2 Score: 152.91 bits (385), Expect = 6.066e-37
Identity = 93/364 (25.55%), Postives = 167/364 (45.88%), Query Frame = -2

Query: 1043 TKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPD-----------------------------------VITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEP 2029
            T L++   ++G  + A   F  +      P ++TYTA++S   ++        L  ++   G++PDS+ F  +IN + ++G++K+A ++   M ++GC P   T+ TL+ G    G  + +++LL  M + G +QPN  TYN ++   C   NIEEA  +V    A+G++ D VTY T+  AY ++GE  +A++++ EM    + P + T  +++ G+C  G + +  + +  M   G+ PN   FNSLVK +    +          M   GV PD                                   V T+S ++  +       + +E+FD M + G+  D   F   +    +   P
Sbjct:  355 TTLIDGFCKRGDIRAASKFFYEMHSRDITPDVLTYTAIISGFCQIGDMVEAGKLFHEMFCKGLEPDSVTFTELINGYCKAGHMKDAFRVHNHMIQAGCSPNVVTYTTLIDGLCKEGDLDSANELLHEMWKIG-LQPNIFTYNSIVNGLCKSGNIEEAVKLVGEFEAAGLNADTVTYTTLMDAYCKSGEMDKAQEILKEMLGKGLQPTIVTFNVLMNGFCLHGMLEDGEKLLNWMLAKGIAPNATTFNSLVKQYCIRNNLKAATAIYKDMCSRGVGPDGKTYENLVKGHCKARNMKEAWFLFQEMKGKGFSVSVSTYSVLIKGFLKRKKFLEAREVFDQMRREGLAADKEIFDFFSDTKYKGKRP 717          

HSP 3 Score: 109.768 bits (273), Expect = 3.130e-23
Identity = 72/296 (24.32%), Postives = 143/296 (48.31%), Query Frame = -2

Query:  710 KLNIEEAWN-----VVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYR-MKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRI 1579
            KLN+ +++      +V+     G  P V  ++   +     G    A ++  +M N  +  +V +C + +T   K+   T     V+R   E+GV  N+  +N ++     +         L LME  G  PDVI++ST++N +   G +DK  ++ + M + G++P+ + +  +     R  +  EAE   + M + G+ P+ V++TT+I G+C  G +  A++ + +M    +TP++ TY  +I G+ +     +A +L   M  KG+  +  T   + + +   G + +A R+
Sbjct:  150 KLNVTDSFVQFFDLLVYTYKDWGSDPRV--FDVFFQVLVDFGLLREARRVFEKMLNYGLVLSVDSCNVYLTRLSKDCYKTATAIIVFREFPEVGVCWNVASYNIVIHFVCQLGRIKEAHHLLLLMELKGYTPDVISYSTVVNGYCRFGELDKVWKLIEVMKRKGLKPNSYIYGSIIGLLCRICKLAEAEEAFSEMIRQGILPDTVVYTTLIDGFCKRGDIRAASKFFYEMHSRDITPDVLTYTAIISGFCQIGDMVEAGKLFHEMFCKGLEPDSVTFTELINGYCKAGHMKDAFRV 443          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy Swiss-Prot
Match: RF1_ORYSI (Protein Rf1, mitochondrial OS=Oryza sativa subsp. indica GN=Rf1 PE=2 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 9.384e-61
Identity = 121/455 (26.59%), Postives = 249/455 (54.73%), Query Frame = -2

Query:  698 GNNSCQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNV 2062
            G  S   V + T ++N   ++G   +A   ++ + + G  P +VTY ++++AL + +     + +++ + +NG+ PD + +N++++ +  SG  KEA+   +KM+  G +P   T++ LM      G+  E+ K+ + M + G ++P   TY  L++ +  K  + E   ++  MV +G+HPD   ++ +  AYA+ G+  +A  +  +M+   + PN  T G ++   CK G + +A+ +  +M + G+ P  +V+NSL+ G          +E +  M + G+  + I F++I+++    G + + +++F+ MV+ G++P+V  ++ L  GY  AG+  EA  LL+ M   G++PN V ++T+I+G+C   +M  A  ++++M   GV+P++ TY  ++ G  + ++   A+EL   + E G   E ST  ++        L ++A ++ +N+
Sbjct:  189 GGGSPPDVVSYTTVINGFFKEGDSDKAYSTYHEMLDRGILPDVVTYNSIIAALCKAQAMDKAMEVLNTMVKNGVMPDCMTYNSILHGYCSSGQPKEAIGFLKKMRSDGVEPDVVTYSLLMDYLCKNGRCMEARKIFDSMTKRG-LKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILICAYAKQGKVDQAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGLSPGNIVYNSLIHGLCTCNKWERAEELILEMLDRGICLNTIFFNSIIDSHCKEGRVIESEKLFELMVRIGVKPNVITYNTLINGYCLAGKMDEAMKLLSGMVSVGLKPNTVTYSTLINGYCKISRMEDALVLFKEMESSGVSPDIITYNIILQGLFQTRRTAAAKELYVRITESGTQIELSTYNIILHGLCKNKLTDDALQMFQNL 642          

HSP 2 Score: 186.422 bits (472), Expect = 9.458e-48
Identity = 110/470 (23.40%), Postives = 225/470 (47.87%), Query Frame = -2

Query:  695 QEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETY-----------------------------------RAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTL---MEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNVD 1990
             +A  + N++ + G  P  +TY +++       + K  I  + K+  +G++PD + ++ +++   ++G   EA KIF  M K G KP  +T+ TL++GY   G   E   LL+LM+++G + P+   ++ILI A+  +  +++A  V  +M   G++P+ VTY  +     ++G                                      RAE++I EM +  +  N      I+  +CKEG + E+ +    M  +GV+PN++ +N+L+ G+        +DEA+ L   M   G+KP+ +T+ST++N +     M+    +F +M  +G+ PD+  ++I+ +G  +      A+ L   + + G Q  +  +  ++ G C     + A ++++ +C + +     T+  +I    +  +  +A++L       G+     T +L+A+     GLL E  ++  +++
Sbjct:  248 DKAMEVLNTMVKNGVMPDCMTYNSILHGYCSSGQPKEAIGFLKKMRSDGVEPDVVTYSLLMDYLCKNGRCMEARKIFDSMTKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNG-IHPDHYVFSILICAYAKQGKVDQAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGLSPGNIVYNSLIHGLCTCNKWERAEELILEMLDRGICLNTIFFNSIIDSHCKEGRVIESEKLFELMVRIGVKPNVITYNTLINGY---CLAGKMDEAMKLLSGMVSVGLKPNTVTYSTLINGYCKISRMEDALVLFKEMESSGVSPDIITYNIILQGLFQTRRTAAAKELYVRITESGTQIELSTYNIILHGLCKNKLTDDALQMFQNLCLMDLKLEARTFNIMIDALLKVGRNDEAKDLFVAFSSNGLVPNYWTYRLMAENIIGQGLLEELDQLFLSME 713          

HSP 3 Score: 178.333 bits (451), Expect = 4.334e-45
Identity = 113/468 (24.15%), Postives = 215/468 (45.94%), Query Frame = -2

Query:  758 ARTKLMNILIEKGKPQEA-QFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEEN---GMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTL---MEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPD-------VHA----------------------------FSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLV 2035
            A T L+  L    +  +A   +   +TE G  P + +Y  L+  L    R +  + L+  + ++   G  PD + +  +IN F + G+  +A   + +M   G  P   T+N+++         +++ ++L  M+++G V P+  TYN ++  +C+    +EA   + +M + G+ PDVVTY+ +     + G    A ++   M    + P + T G ++ GY  +G + E    +  M   G+ P+  VF+ L+  +        VD+A+ +   M + G+ P+ +T+  ++     +G ++     F+ M+  G+ P        +H                             F+ +   + + G   E+E L   M + GV+PNV+ + T+I+G+C AGKM+ A ++   M  +G+ PN  TY TLI GY +  +   A  L + ME  G+S +  T  ++
Sbjct:  124 AFTPLLKGLCADKRTSDAMDIVLRRMTELGCIPNVFSYNILLKGLCDENRSQEALELLHMMADDRGGGSPPDVVSYTTVINGFFKEGDSDKAYSTYHEMLDRGILPDVVTYNSIIAALCKAQAMDKAMEVLNTMVKNG-VMPDCMTYNSILHGYCSSGQPKEAIGFLKKMRSDGVEPDVVTYSLLMDYLCKNGRCMEARKIFDSMTKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILICAY---AKQGKVDQAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGLSPGNIVYNSLIHGLCTCNKWERAEELILEMLDRGICLNTIFFNSIIDSHCKEGRVIESEKLFELMVRIGVKPNVITYNTLINGYCLAGKMDEAMKLLSGMVSVGLKPNTVTYSTLINGYCKISRMEDALVLFKEMESSGVSPDIITYNII 587          

HSP 4 Score: 166.392 bits (420), Expect = 3.517e-41
Identity = 111/459 (24.18%), Postives = 206/459 (44.88%), Query Frame = -2

Query:  752 LMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWC--NK-----------------LN----------------IEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVAD 2023
            LM+ L + G+  EA+ IF+S+T+ G KP + TY  L+           +  L+  +  NG+ PD  +F+ +I A+++ G V +AM +F KM++ G  P   T+  ++      G+ E++    E M+ +G + P +  YN LI   C  NK                 LN                + E+  +   MV  G+ P+V+TYNT+   Y   G+   A +++  M +  + PN  T   ++ GYCK   M +AL     M+  GV P+++ +N +++G      T    E    + E G + ++ T++ I++      L D   ++F ++    ++ +   F+I+    ++ G   EA+ L  +    G+ PN   +  +       G +    +++  M + G T +      ++    +  +  +A   L +++EK  S E ST  L  D
Sbjct:  307 LMDYLCKNGRCMEARKIFDSMTKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILICAYAKQGKVDQAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEG-LSPGNIVYNSLIHGLCTCNKWERAEELILEMLDRGICLNTIFFNSIIDSHCKEGRVIESEKLFELMVRIGVKPNVITYNTLINGYCLAGKMDEAMKLLSGMVSVGLKPNTVTYSTLINGYCKISRMEDALVLFKEMESSGVSPDIITYNIILQGLFQTRRTAAAKELYVRITESGTQIELSTYNIILHGLCKNKLTDDALQMFQNLCLMDLKLEARTFNIMIDALLKVGRNDEAKDLFVAFSSNGLVPNYWTYRLMAENIIGQGLLEELDQLFLSMEDNGCTVDSGMLNFIVRELLQRGEITRAGTYLSMIDEKHFSLEASTASLFID 764          

HSP 5 Score: 147.132 bits (370), Expect = 5.178e-35
Identity = 82/308 (26.62%), Postives = 148/308 (48.05%), Query Frame = -2

Query:  719 VQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIF-EMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKEL---GVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEA 1630
            V P+  TY ILI   C    ++  +  +  ++  G   D + +  + +       T  A  ++   M      PNV +  I++ G C E    EAL  ++ M +    G  P++V + +++ GF    D++        M + G+ PDV+T+++I+ A   A  MDK  E+ + MVK G+ PD   ++ +  GY  +G+P EA   L  M   GV+P+VV ++ ++   C  G+   A ++++ M + G+ P + TY TL+ GY       +   LL +M   GI  +     ++  A+   G +++A
Sbjct:   83 VTPDLCTYGILIGCCCRAGRLDLGFAALGNVIKKGFRVDAIAFTPLLKGLCADKRTSDAMDIVLRRMTELGCIPNVFSYNILLKGLCDENRSQEALELLHMMADDRGGGSPPDVVSYTTVINGFFKEGDSDKAYSTYHEMLDRGILPDVVTYNSIIAALCKAQAMDKAMEVLNTMVKNGVMPDCMTYNSILHGYCSSGQPKEAIGFLKKMRSDGVEPDVVTYSLLMDYLCKNGRCMEARKIFDSMTKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILICAYAKQGKVDQA 390          

HSP 6 Score: 106.301 bits (264), Expect = 3.804e-22
Identity = 72/281 (25.62%), Postives = 130/281 (46.26%), Query Frame = -2

Query:  686 VVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFL-DVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVK---AGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNV-DRGI 1513
            V  YN +ARA A                 ++V P++ T GI++   C+ G +      +  + + G + + + F  L+KG   D   ++ +D  L  M E G  P+V +++ ++          +  E+   M      G  PDV +++ +  G+ + G+  +A S  + M   G+ P+VV + ++I+  C A  M+ A  V   M + GV P+  TY +++ GY  + QP +A   L+ M   G+  +  T  L+ D     G   EAR+I  ++  RG+
Sbjct:   69 VSRYNRMARAGA-----------------DEVTPDLCTYGILIGCCCRAGRLDLGFAALGNVIKKGFRVDAIAFTPLLKGLCADKRTSDAMDIVLRRMTELGCIPNVFSYNILLKGLCDENRSQEALELLHMMADDRGGGSPPDVVSYTTVINGFFKEGDSDKAYSTYHEMLDRGILPDVVTYNSIIAALCKAQAMDKAMEVLNTMVKNGVMPDCMTYNSILHGYCSSGQPKEAIGFLKKMRSDGVEPDVVTYSLLMDYLCKNGRCMEARKIFDSMTKRGL 332          

HSP 7 Score: 62.3882 bits (150), Expect = 1.561e-8
Identity = 43/192 (22.40%), Postives = 87/192 (45.31%), Query Frame = -2

Query: 1454 TKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRA 2029
            + L+N   +  + ++A  +F  +   G  P ++TY  ++  L + +R  +   L  ++ E+G + +   +N +++   ++    +A+++FQ +     K    TFN ++     +G+ +E+  L      +G V PN  TY ++      +  +EE   +   M  +G   D    N I R   Q GE  RA
Sbjct:  550 STLINGYCKISRMEDALVLFKEMESSGVSPDIITYNIILQGLFQTRRTAAAKELYVRITESGTQIELSTYNIILHGLCKNKLTDDALQMFQNLCLMDLKLEARTFNIMIDALLKVGRNDEAKDLFVAFSSNGLV-PNYWTYRLMAENIIGQGLLEELDQLFLSMEDNGCTVDSGMLNFIVRELLQRGEITRA 740          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy Swiss-Prot
Match: PPR96_ARATH (Pentatricopeptide repeat-containing protein At1g62930, chloroplastic OS=Arabidopsis thaliana GN=At1g62930 PE=2 SV=2)

HSP 1 Score: 222.631 bits (566), Expect = 9.912e-61
Identity = 123/436 (28.21%), Postives = 225/436 (51.61%), Query Frame = -2

Query:  716 LMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEAR 2023
            L++ L    K  EA  + + +   G +P L TY  +V+ L +       +SL+ K+E+  ++ D +++  +I+A     NV +A+ +F +M   G +P   T+N+L++   N G+  ++S+LL  M++   + PN  T++ LI A+  +  + EA  +   M+   + PD+ TY+++   +        A+ M   M +    PNV T   ++ G+CK   + E +     M + G+  N V +N+L++G     D +   +    M   GV PD+IT+S +++     G ++K   +F+ + K+ +EPD++ ++I+ +G  +AG+  +   L  S+   GV+PNV+I+TT+ISG+C  G    A  ++ +M E G  PN  TY TLI           + EL++ M   G   + STI +V      I +L++ R
Sbjct:  191 LIHGLFLHNKASEAVALIDRMVARGCQPDLFTYGTVVNGLCKRGDIDLALSLLKKMEKGKIEADVVIYTTIIDALCNYKNVNDALNLFTEMDNKGIRPNVVTYNSLIRCLCNYGRWSDASRLLSDMIER-KINPNVVTFSALIDAFVKEGKLVEAEKLYDEMIKRSIDPDIFTYSSLINGFCMHDRLDEAKHMFELMISKDCFPNVVTYNTLIKGFCKAKRVEEGMELFREMSQRGLVGNTVTYNTLIQGLFQAGDCDMAQKIFKKMVSDGVPPDIITYSILLDGLCKYGKLEKALVVFEYLQKSKMEPDIYTYNIMIEGMCKAGKVEDGWDLFCSLSLKGVKPNVIIYTTMISGFCRKGLKEEADALFREMKEDGTLPNSGTYNTLIRARLRDGDKAASAELIKEMRSCGFVGDASTISMV------INMLHDGR 619          

HSP 2 Score: 204.912 bits (520), Expect = 9.516e-55
Identity = 118/438 (26.94%), Postives = 218/438 (49.77%), Query Frame = -2

Query:  719 RTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEA 2032
            R KL   ++   K  +A  +F  + +    P++V +  L+SA+ ++ ++  +ISL  +++   +  D   +N +IN F     +  A+ +  KM K G +P   T ++L+ GY +  +  E+  L++ M      QPN  T+N LI          EA  ++ RMVA G  PD+ TY T+     + G+   A  ++ +M+  K+  +V     I+   C   N+ +AL     M   G++PN+V +NSL++   +    +     L+ M E  + P+V+TFS +++A+   G + + ++++D+M+K  I+PD+  +S L  G+       EA+ +   M      PNVV + T+I G+C A ++     ++ +M + G+  N  TY TLI G  +A     A+++ + M   G+  +  T  ++ D     G L +A
Sbjct:   48 REKLSRNVLLDLKLDDAVDLFGEMVQSRPLPSIVEFNKLLSAIAKMNKFDLVISLGERMQNLRISYDLYSYNILINCFCRRSQLPLALAVLGKMMKLGYEPDIVTLSSLLNGYCHGKRISEAVALVDQMFV-MEYQPNTVTFNTLIHGLFLHNKASEAVALIDRMVARGCQPDLFTYGTVVNGLCKRGDIDLALSLLKKMEKGKIEADVVIYTTIIDALCNYKNVNDALNLFTEMDNKGIRPNVVTYNSLIRCLCNYGRWSDASRLLSDMIERKINPNVVTFSALIDAFVKEGKLVEAEKLYDEMIKRSIDPDIFTYSSLINGFCMHDRLDEAKHMFELMISKDCFPNVVTYNTLIKGFCKAKRVEEGMELFREMSQRGLVGNTVTYNTLIQGLFQAGDCDMAQKIFKKMVSDGVPPDIITYSILLDGLCKYGKLEKA 484          

HSP 3 Score: 82.4185 bits (202), Expect = 7.687e-15
Identity = 58/216 (26.85%), Postives = 98/216 (45.37%), Query Frame = -2

Query:  674 PNLVVFNSLVKGFLDVTDTNGVDEALTL---MEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNVD-RGIASNT 1309
            P++V FN L+     +   N  D  ++L   M+   +  D+ +++ ++N +     +     +   M+K G EPD+   S L  GY       EA +L++ M     QPN V F T+I G     K + A  + ++M   G  P+L TY T++ G  +      A  LL+ ME+  I A+      + DA      +N+A  +   +D +GI  N 
Sbjct:   78 PSIVEFNKLLSA---IAKMNKFDLVISLGERMQNLRISYDLYSYNILINCFCRRSQLPLALAVLGKMMKLGYEPDIVTLSSLLNGYCHGKRISEAVALVDQMFVMEYQPNTVTFNTLIHGLFLHNKASEAVALIDRMVARGCQPDLFTYGTVVNGLCKRGDIDLALSLLKKMEKGKIEADVVIYTTIIDALCNYKNVNDALNLFTEMDNKGIRPNV 290          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy Swiss-Prot
Match: PPR38_ARATH (Putative pentatricopeptide repeat-containing protein At1g12700, mitochondrial OS=Arabidopsis thaliana GN=At1g12700 PE=3 SV=1)

HSP 1 Score: 216.468 bits (550), Expect = 8.287e-59
Identity = 119/420 (28.33%), Postives = 224/420 (53.33%), Query Frame = -2

Query:  752 LIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVAD 2011
            L  +GK  EA  + + + E G +P +VTY ++V+ + R       + L+ K+EE  +K D   ++ +I++    G +  A+ +F++M+  G K +  T+N+L++G    GK  + + LL+ M+    + PN  T+N+L+  +  +  ++EA  +   M+  G+ P+++TYNT+   Y        A  M+  M  NK +P++ T   ++ GYC    + + ++    + + G+  N V ++ LV+GF         +E    M   GV PDV+T+  +++     G ++K  EIF+D+ K+ ++  +  ++ + +G  + G+  +A +L  S+   GV+PNV+ +T +ISG C  G ++ A  +  KM E G  PN  TY TLI  +        + +L++ M+  G SA+ S+I++V D
Sbjct:  168 LFLEGKVSEAVVLVDRMVENGCQPDVVTYNSIVNGICRSGDTSLALDLLRKMEERNVKADVFTYSTIIDSLCRDGCIDAAISLFKEMETKGIKSSVVTYNSLVRGLCKAGKWNDGALLLKDMVSR-EIVPNVITFNVLLDVFVKEGKLQEANELYKEMITRGISPNIITYNTLMDGYCMQNRLSEANNMLDLMVRNKCSPDIVTFTSLIKGYCMVKRVDDGMKVFRNISKRGLVANAVTYSILVQGFCQSGKIKLAEELFQEMVSHGVLPDVMTYGILLDGLCDNGKLEKALEIFEDLQKSKMDLGIVMYTTIIEGMCKGGKVEDAWNLFCSLPCKGVKPNVMTYTVMISGLCKKGSLSEANILLRKMEEDGNAPNDCTYNTLIRAHLRDGDLTASAKLIEEMKSCGFSADASSIKMVID 586          

HSP 2 Score: 191.045 bits (484), Expect = 3.205e-50
Identity = 120/450 (26.67%), Postives = 217/450 (48.22%), Query Frame = -2

Query:  746 SGNNSCQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEK----GISAERSTIQ------LVADAW 2065
            S  N C   R R+ +++I     K  +A  +F  +      P+LV ++   SA+ R K++  ++    ++E NG+  +    N MIN F        A  +  K+ K G +P T+TFNTL+KG    GK  E+  L++ M+++G  QP+  TYN ++   C   +   A +++ +M    +  DV TY+TI  +  + G    A  +  EM+   +  +V T   +V G CK G   +    +  M    + PN++ FN L+  F+        +E    M   G+ P++IT++T+M+ +     + +   + D MV+    PD+  F+ L KGY       +   +  ++ K G+  N V ++ ++ G+C +GK+  A  ++++M   GV P++ TY  L+ G  +  +  KA E+ + +++     GI    + I+       V DAW
Sbjct:   50 SNGNVCFRERLRSGIVDI-----KKDDAIALFQEMIRSRPLPSLVDFSRFFSAIARTKQFNLVLDFCKQLELNGIAHNIYTLNIMINCFCRCCKTCFAYSVLGKVMKLGYEPDTTTFNTLIKGLFLEGKVSEAVVLVDRMVENG-CQPDVVTYNSIVNGICRSGDTSLALDLLRKMEERNVKADVFTYSTIIDSLCRDGCIDAAISLFKEMETKGIKSSVVTYNSLVRGLCKAGKWNDGALLLKDMVSREIVPNVITFNVLLDVFVKEGKLQEANELYKEMITRGISPNIITYNTLMDGYCMQNRLSEANNMLDLMVRNKCSPDIVTFTSLIKGYCMVKRVDDGMKVFRNISKRGLVANAVTYSILVQGFCQSGKIKLAEELFQEMVSHGVLPDVMTYGILLDGLCDNGKLEKALEIFEDLQKSKMDLGIVMYTTIIEGMCKGGKVEDAW 493          

HSP 3 Score: 128.642 bits (322), Expect = 1.502e-29
Identity = 75/294 (25.51%), Postives = 144/294 (48.98%), Query Frame = -2

Query: 1142 LMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDK 2023
            L+++ +++GK QEA  ++  +   G  P ++TY  L+       R     +++  +  N   PD + F ++I  +     V + MK+F+ + K G      T++ L++G+   GK + + +L + M+  G V P+  TY IL+   C+   +E+A  +   +  S M   +V Y TI     + G+   A  +   +    V PNV T  ++++G CK+G+++EA   + +M+E G  PN   +N+L++  L   D     + +  M+  G   D  +   +++   S G +DK
Sbjct:  304 LLDVFVKEGKLQEANELYKEMITRGISPNIITYNTLMDGYCMQNRLSEANNMLDLMVRNKCSPDIVTFTSLIKGYCMVKRVDDGMKVFRNISKRGLVANAVTYSILVQGFCQSGKIKLAEELFQEMVSHG-VLPDVMTYGILLDGLCDNGKLEKALEIFEDLQKSKMDLGIVMYTTIIEGMCKGGKVEDAWNLFCSLPCKGVKPNVMTYTVMISGLCKKGSLSEANILLRKMEEDGNAPNDCTYNTLIRAHLRDGDLTASAKLIEEMKSCGFSADASSIKMVIDMLLS-GELDK 595          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy Swiss-Prot
Match: PP407_ARATH (Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana GN=EMB2745 PE=2 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 1.817e-58
Identity = 124/417 (29.74%), Postives = 217/417 (52.04%), Query Frame = -2

Query:  707 EGHKPTLVTYTALVSALTRLKRYKSII-SLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRII 1954
             G  P +++Y A++ A  R KR  S   ++  ++ E+ + P+   +N +I  F  +GN+  A+ +F KM+  GC P   T+NTL+ GY  + K ++  KLL  M   G ++PN  +YN++I   C +  ++E   V+  M   G   D VTYNT+ + Y + G  ++A  M  EM  + + P+V T   ++   CK GNM  A+ F+ +M+  G+ PN   + +LV GF      N     L  M + G  P V+T++ ++N     G M+    + +DM + G+ PDV ++S +  G+ R+ +  EA  +   M + G++P+ + ++++I G+C   +   A  +YE+M  +G+ P+  TY  LI  Y       KA +L   M EKG+  +  T  ++ +         EA+R++
Sbjct:  163 HGFMPGVLSYNAVLDATIRSKRNISFAENVFKEMLESQVSPNVFTYNILIRGFCFAGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKIDDGFKLLRSMALKG-LEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSRTREAKRLL 578          

HSP 2 Score: 197.208 bits (500), Expect = 1.119e-51
Identity = 120/446 (26.91%), Postives = 222/446 (49.78%), Query Frame = -2

Query:  692 IFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEF---GVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVIS---------------GWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNVDR 1975
            +  S+  +G +P L++Y  +++ L R  R K +  +++++   G   D + +N +I  + + GN  +A+ +  +M + G  P+  T+ +L+      G    + + L+ M   G + PN+RTY  L+  +  K  + EA+ V+  M  +G  P VVTYN +   +  TG+   A  ++ +M+   ++P+V +   +++G+C+  ++ EALR    M E G++P+ + ++SL++GF +   T    EA  L EE    G+ PD  T++ ++NA+   G ++K  ++ ++MV+ G+ PDV  +S+L  G  +     EA+ LL  +      P+ V + T+I                G+C  G M  A +V+E M      P+   Y  +I G+  A    KA  L + M + G      T+  +  A    G +NE   +I +V R
Sbjct:  262 LLRSMALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRG-LCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCEQRRTK---EACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLIENCSNIEFKSVVSLIKGFCMKGMMTEADQVFESMLGKNHKPDGTAYNIMIHGHCRAGDIRKAYTLYKEMVKSGFLLHTVTVIALVKALHKEGKVNELNSVIVHVLR 703          

HSP 3 Score: 145.591 bits (366), Expect = 1.419e-34
Identity = 82/313 (26.20%), Postives = 166/313 (53.04%), Query Frame = -2

Query:  677 YNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYR-AEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNV-DRGIASN 1609
            ++++++++     I++A ++VH   A G  P V++YN +  A  ++      AE +  EM  ++V+PNV T  I++ G+C  GN+  AL    +M+  G  PN+V +N+L+ G+  +   +   + L  M   G++P++I+++ ++N     G M +   +  +M + G   D   ++ L KGY + G   +A  +   M + G+ P+V+ +T++I   C AG MN A    ++M   G+ PN  TY TL+ G+ +     +A  +L+ M + G S    T   + +     G + +A  +++++ ++G++ +
Sbjct:  137 FDLVVKSYSRLSLIDKALSIVHLAQAHGFMPGVLSYNAVLDATIRSKRNISFAENVFKEMLESQVSPNVFTYNILIRGFCFAGNIDVALTLFDKMETKGCLPNVVTYNTLIDGYCKLRKIDDGFKLLRSMALKGLEPNLISYNVVINGLCREGRMKEVSFVLTEMNRRGYSLDEVTYNTLIKGYCKEGNFHQALVMHAEMLRHGLTPSVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPD 449          

HSP 4 Score: 137.887 bits (346), Expect = 4.009e-32
Identity = 86/370 (23.24%), Postives = 178/370 (48.11%), Query Frame = -2

Query:  935 TVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMN 2044
            +V   T L++ + + G    A    + +   G  P   TYT LV   ++         ++ ++ +NG  P  + +NA+IN    +G +++A+ + + MK+ G  P   +++T++ G+      +E+ ++   M++ G ++P+  TY+ LI+ +C +   +EA ++   M+  G+ PD  TY  +  AY   G+  +A Q+  EM    V P+V T  +++ G  K+    EA R + ++      P+ V +++L++   ++              EF       +  +++  +   G+M +  ++F+ M+    +PD  A++I+  G+ RAG+  +A +L   M K G   + V    ++      GK+N
Sbjct:  344 SVITYTSLIHSMCKAGNMNRAMEFLDQMRVRGLCPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKG-IKPDTITYSSLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLIENCSNI--------------EFK------SVVSLIKGFCMKGMMTEADQVFESMLGKNHKPDGTAYNIMIHGHCRAGDIRKAYTLYKEMVKSGFLLHTVTVIALVKALHKEGKVN 692          

HSP 5 Score: 134.035 bits (336), Expect = 6.921e-31
Identity = 91/372 (24.46%), Postives = 182/372 (48.92%), Query Frame = -2

Query:  989 CQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPN-------VRTCGII--------VTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEF---GVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPN 2050
            C   R  T L++   +KG   EA  +   + + G  P++VTY AL++      + +  I+++  ++E G+ PD + ++ +++ F  S +V EA+++ ++M + G KP T T+++L++G+    + +E+  L E ML+ G + P++ TY  LI A+C + ++E+A  + + MV  G+ PDVVTY+ +     +   T  A++++ ++   +  P+       +  C  I        + G+C +G MTEA +    M     +P+   +N ++ G     D   + +A TL +E    G     +T   ++ A    G +++   +   ++++    +     +L +   R G       +L  M K G  PN
Sbjct:  377 CPNERTYTTLVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALINGHCVTGKMEDAIAVLEDMKEKGLSPDVVSYSTVLSGFCRSYDVDEALRVKREMVEKGIKPDTITYSSLIQGFCEQRRTKEACDLYEEMLRVG-LPPDEFTYTALINAYCMEGDLEKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTLIENCSNIEFKSVVSLIKGFCMKGMMTEADQVFESMLGKNHKPDGTAYNIMIHGHCRAGD---IRKAYTLYKEMVKSGFLLHTVTVIALVKALHKEGKVNELNSVIVHVLRSCELSEAEQAKVLVEINHREGNMDVVLDVLAEMAKDGFLPN 744          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy Swiss-Prot
Match: PP247_ARATH (Pentatricopeptide repeat-containing protein At3g22470, mitochondrial OS=Arabidopsis thaliana GN=At3g22470 PE=2 SV=1)

HSP 1 Score: 213.772 bits (543), Expect = 9.304e-58
Identity = 121/426 (28.40%), Postives = 221/426 (51.88%), Query Frame = -2

Query:  752 TKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVAD 2029
            + L+N L  KG+  EA  + + + E G +P  VTY  +++ L +       + L  K+EE  +K   + ++ +I++  + G+  +A+ +F +M+  G K    T+++L+ G  N GK ++ +K+L  M+   N+ P+  T++ LI  +  +  + EA  + + M+  G+ PD +TYN++   + +    + A QM   M +    P++ T  I++  YCK   + + +R    +   G+ PN + +N+LV GF      N   E    M   GV P V+T+  +++     G ++K  EIF+ M K+ +   +  ++I+  G   A +  +A SL  S+   GV+P+VV +  +I G C  G ++ A  ++ KM E G TP+  TY  LI  +        + EL++ M+  G SA+ STI++V D
Sbjct:  179 STLINGLCLKGRVSEALVLIDRMVEYGFQPDEVTYGPVLNRLCKSGNSALALDLFRKMEERNIKASVVQYSIVIDSLCKDGSFDDALSLFNEMEMKGIKADVVTYSSLIGGLCNDGKWDDGAKMLREMIGR-NIIPDVVTFSALIDVFVKEGKLLEAKELYNEMITRGIAPDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDIVTYSILINSYCKAKRVDDGMRLFREISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRGVPPSVVTYGILLDGLCDNGELNKALEIFEKMQKSRMTLGIGIYNIIIHGMCNASKVDDAWSLFCSLSDKGVKPDVVTYNVMIGGLCKKGSLSEADMLFRKMKEDGCTPDDFTYNILIRAHLGGSGLISSVELIEEMKVCGFSADSSTIKMVID 603          

HSP 2 Score: 204.527 bits (519), Expect = 1.273e-54
Identity = 121/451 (26.83%), Postives = 230/451 (51.00%), Query Frame = -2

Query:  692 RARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFG--VKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNVDR 2038
            R R  +++I     K  +A  +F S+ +    PT + +  L SA+ R K+Y  ++     +E NG++ D      MIN +     +  A  +  +  K G +P T TF+TL+ G+   G+  E+  L++ M++    +P+  T + LI   C K  + EA  ++ RMV  G  PD VTY  +     ++G +  A  +  +M+   +  +V    I++   CK+G+  +AL     M+  G++ ++V ++SL+ G  +  D    D A  L E  G  + PDV+TFS +++ +   G + + +E++++M+  GI PD   ++ L  G+ +     EA  + + M   G +P++V ++ +I+ +C A +++   R++ ++   G+ PN  TY TL+ G+ ++ +   A+EL Q M  +G+     T  ++ D     G LN+A  I + + +
Sbjct:   41 RLRNGIVDI-----KVNDAIDLFESMIQSRPLPTPIDFNRLCSAVARTKQYDLVLGFCKGMELNGIEHDMYTMTIMINCYCRKKKLLFAFSVLGRAWKLGYEPDTITFSTLVNGFCLEGRVSEAVALVDRMVEMKQ-RPDLVTVSTLINGLCLKGRVSEALVLIDRMVEYGFQPDEVTYGPVLNRLCKSGNSALALDLFRKMEERNIKASVVQYSIVIDSLCKDGSFDDALSLFNEMEMKGIKADVVTYSSLIGGLCN--DGKWDDGAKMLREMIGRNIIPDVVTFSALIDVFVKEGKLLEAKELYNEMITRGIAPDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDIVTYSILINSYCKAKRVDDGMRLFREISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRGVPPSVVTYGILLDGLCDNGELNKALEIFEKMQK 483          

HSP 3 Score: 181.415 bits (459), Expect = 8.583e-47
Identity = 108/446 (24.22%), Postives = 219/446 (49.10%), Query Frame = -2

Query:  698 TKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELM--LQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNV 2029
            T ++N    K K   A  +     + G++P  +T++ LV+      R    ++L+ ++ E   +PD +  + +IN     G V EA+ +  +M + G +P   T+  ++     + K+  S+  L+L   +++ N++ +   Y+I+I + C   + ++A ++ + M   G+  DVVTY+++       G+     +M+ EM    + P+V T   ++  + KEG + EA      M   G+ P+ + +NSL+ GF      +  ++   LM   G +PD++T+S ++N++  A  +D    +F ++   G+ P+   ++ L  G+ ++G+   A+ L   M   GV P+VV +  ++ G C  G++N A  ++EKM +  +T  +  Y  +I G   A +   A  L   + +KG+  +  T  ++       G L+EA  + + +
Sbjct:  109 TIMINCYCRKKKLLFAFSVLGRAWKLGYEPDTITFSTLVNGFCLEGRVSEAVALVDRMVEMKQRPDLVTVSTLINGLCLKGRVSEALVLIDRMVEYGFQPDEVTYGPVLN---RLCKSGNSALALDLFRKMEERNIKASVVQYSIVIDSLCKDGSFDDALSLFNEMEMKGIKADVVTYSSLIGGLCNDGKWDDGAKMLREMIGRNIIPDVVTFSALIDVFVKEGKLLEAKELYNEMITRGIAPDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDIVTYSILINSYCKAKRVDDGMRLFREISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRGVPPSVVTYGILLDGLCDNGELNKALEIFEKMQKSRMTLGIGIYNIIIHGMCNASKVDDAWSLFCSLSDKGVKPDVVTYNVMIGGLCKKGSLSEADMLFRKM 551          

HSP 4 Score: 128.642 bits (322), Expect = 1.682e-29
Identity = 77/307 (25.08%), Postives = 149/307 (48.53%), Query Frame = -2

Query: 1151 GNNSCQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEF---GVKPDVITFSTIMNAWSSAGL 2062
            G N    V   + L+++ +++GK  EA+ ++N +   G  P  +TY +L+    +         +   +   G +PD + ++ +IN++ ++  V + M++F+++   G  P T T+NTL+ G+   GK   + +L + M+  G V P+  TY IL+   C+   + +A  +  +M  S M   +  YN I        +   A  +   + +  V P+V T  +++ G CK+G+++EA     +MKE G  P+   +N L++  L     +G+  ++ L+EE    G   D  T   +++  S   L
Sbjct:  308 GRNIIPDVVTFSALIDVFVKEGKLLEAKELYNEMITRGIAPDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDIVTYSILINSYCKAKRVDDGMRLFREISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRG-VPPSVVTYGILLDGLCDNGELNKALEIFEKMQKSRMTLGIGIYNIIIHGMCNASKVDDAWSLFCSLSDKGVKPDVVTYNVMIGGLCKKGSLSEADMLFRKMKEDGCTPDDFTYNILIRAHL---GGSGLISSVELIEEMKVCGFSADSSTIKMVIDMLSDRRL 610          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy Swiss-Prot
Match: PPR98_ARATH (Pentatricopeptide repeat-containing protein At1g63080, mitochondrial OS=Arabidopsis thaliana GN=At1g63080 PE=2 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 1.374e-57
Identity = 114/426 (26.76%), Postives = 226/426 (53.05%), Query Frame = -2

Query:  752 TKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVAD 2029
            T L++ L +  K  EA  +   +  +G +P LVTY A+++ L +       ++L++K+E+  ++ D ++++ +I++  +  +V +A+ +F +M   G +P   T+++L+    N G+  ++S+LL  ML+   + PN  T+N LI A+  +  + EA  +   M+   + P++VTYN++   +        A+Q+   M +    P+V T   ++ G+CK   + + +     M   G+  N V + +L+ GF   +D +        M   GV P+++T++T+++     G ++K   +F+ + K+ +EPD++ ++I+++G  +AG+  +   L  S+   GV+P+V+ + T+ISG+C  G    A  ++ KM E G  P+  TY TLI  +        + EL++ M     + + ST  LV D
Sbjct:  174 TTLVHGLFQHNKASEAVALVERMVVKGCQPDLVTYGAVINGLCKRGEPDLALNLLNKMEKGKIEADVVIYSTVIDSLCKYRHVDDALNLFTEMDNKGIRPDVFTYSSLISCLCNYGRWSDASRLLSDMLER-KINPNVVTFNSLIDAFAKEGKLIEAEKLFDEMIQRSIDPNIVTYNSLINGFCMHDRLDEAQQIFTLMVSKDCLPDVVTYNTLINGFCKAKKVVDGMELFRDMSRRGLVGNTVTYTTLIHGFFQASDCDNAQMVFKQMVSDGVHPNIMTYNTLLDGLCKNGKLEKAMVVFEYLQKSKMEPDIYTYNIMSEGMCKAGKVEDGWDLFCSLSLKGVKPDVIAYNTMISGFCKKGLKEEAYTLFIKMKEDGPLPDSGTYNTLIRAHLRDGDKAASAELIKEMRSCRFAGDASTYGLVTD 598          

HSP 2 Score: 194.897 bits (494), Expect = 1.945e-51
Identity = 107/435 (24.60%), Postives = 214/435 (49.20%), Query Frame = -2

Query:  719 LMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEA 2023
            ++N L  + +   A  I   + + G+ P++VT  +L++      R    ++L+ ++ E G +PD++ F  +++   +     EA+ + ++M   GC+P   T+  ++ G    G+ + +  LL  M + G ++ +   Y+ +I + C   ++++A N+   M   G+ PDV TY+++       G    A +++ +M   K+ PNV T   ++  + KEG + EA +    M +  + PN+V +NSL+ GF      +   +  TLM      PDV+T++T++N +  A  +    E+F DM + G+  +   ++ L  G+ +A +   A+ +   M   GV PN++ + T++ G C  GK+  A  V+E + +  + P++ TY  +  G  +A +     +L   +  KG+  +      +   +   GL  EA
Sbjct:  106 MINCLCRRSQLSFALAILGKMMKLGYGPSIVTLNSLLNGFCHGNRISEAVALVDQMVEMGYQPDTVTFTTLVHGLFQHNKASEAVALVERMVVKGCQPDLVTYGAVINGLCKRGEPDLALNLLNKM-EKGKIEADVVIYSTVIDSLCKYRHVDDALNLFTEMDNKGIRPDVFTYSSLISCLCNYGRWSDASRLLSDMLERKINPNVVTFNSLIDAFAKEGKLIEAEKLFDEMIQRSIDPNIVTYNSLINGFCMHDRLDEAQQIFTLMVSKDCLPDVVTYNTLINGFCKAKKVVDGMELFRDMSRRGLVGNTVTYTTLIHGFFQASDCDNAQMVFKQMVSDGVHPNIMTYNTLLDGLCKNGKLEKAMVVFEYLQKSKMEPDIYTYNIMSEGMCKAGKVEDGWDLFCSLSLKGVKPDVIAYNTMISGFCKKGLKEEA 539          

HSP 3 Score: 194.512 bits (493), Expect = 2.464e-51
Identity = 114/447 (25.50%), Postives = 221/447 (49.44%), Query Frame = -2

Query:  692 RTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNVDR 2032
            R KL    +   K  EA  +F  + +    P++V ++ L+SA+ ++K++  +IS   K+E  G+  +   +N MIN       +  A+ I  KM K G  P+  T N+L+ G+ +  +  E+  L++ M++ G  QP+  T+  L+          EA  +V RMV  G  PD+VTY  +     + GE   A  ++ +M+  K+  +V     ++   CK  ++ +AL     M   G++P++  ++SL+    +    +     L+ M E  + P+V+TF+++++A++  G + + +++FD+M++  I+P++  ++ L  G+       EA+ +   M      P+VV + T+I+G+C A K+     ++  M   G+  N  TY TLI G+ +A     A+ + + M   G+     T   + D     G L +A  + + + +
Sbjct:   33 REKLSRNALLHLKLDEAVDLFGEMVKSRPFPSIVEFSKLLSAIAKMKKFDLVISFGEKMEILGVSHNLYTYNIMINCLCRRSQLSFALAILGKMMKLGYGPSIVTLNSLLNGFCHGNRISEAVALVDQMVEMG-YQPDTVTFTTLVHGLFQHNKASEAVALVERMVVKGCQPDLVTYGAVINGLCKRGEPDLALNLLNKMEKGKIEADVVIYSTVIDSLCKYRHVDDALNLFTEMDNKGIRPDVFTYSSLISCLCNYGRWSDASRLLSDMLERKINPNVVTFNSLIDAFAKEGKLIEAEKLFDEMIQRSIDPNIVTYNSLINGFCMHDRLDEAQQIFTLMVSKDCLPDVVTYNTLINGFCKAKKVVDGMELFRDMSRRGLVGNTVTYTTLIHGFFQASDCDNAQMVFKQMVSDGVHPNIMTYNTLLDGLCKNGKLEKAMVVFEYLQK 478          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy Swiss-Prot
Match: PP445_ARATH (Pentatricopeptide repeat-containing protein At5g65560 OS=Arabidopsis thaliana GN=At5g65560 PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 2.862e-57
Identity = 141/556 (25.36%), Postives = 267/556 (48.02%), Query Frame = -2

Query:  524 CQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLA-----------------------------------TYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNV-DRGI--ASNTKDDIPAEGMEKTYKKQSLS----ASYSNVLQMPGVA-----MNEQNGSSSSKARSQMVL 2050
            C  +    K++N   + G  +EA    + + E G  P   TYT+L+    + K   S   + +++   G + + + +  +I+    +  + EAM +F KMK   C PT  T+  L+K      +  E+  L++ M + G ++PN  TY +LI + C++   E+A  ++ +M+  G+ P+V+TYN +   Y + G    A  ++  M++ K++PN RT   ++ GYCK  N+ +A+  + +M E  V P++V +NSL+ G     + +     L+LM + G+ PD  T+++++++   +  +++  ++FD + + G+ P+V  ++ L  GY +AG+  EA  +L  M      PN + F  +I G C  GK+  A  + EKM +IG+ P ++                                   TY T I  Y    +   AE+++  M E G+S +  T   +   +  +G  N A  ++K + D G   + +T   +    +E  Y KQ  S     + SN+++   V      M E + + ++K+  +++L
Sbjct:  215 CPNIYTYNKMVNGYCKLGNVEEANQYVSKIVEAGLDPDFFTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSERKSEALNLVKEMEETG-IKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCK-SNVHKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLLKDGDFDHAYSRFQQMLSSGTKPDAHTYTTFIQTYCREGRLLDAEDMMAKMRENGVSPDLFTYSSLIKGYGDLGQTNFAFDVLKRMRDTGCEPSQHTFLSLIKHLLEMKYGKQKGSEPELCAMSNMMEFDTVVELLEKMVEHSVTPNAKSYEKLIL 768          

HSP 2 Score: 188.734 bits (478), Expect = 3.960e-48
Identity = 127/475 (26.74%), Postives = 227/475 (47.79%), Query Frame = -2

Query:  692 TVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGY--GNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEE----FGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLI-------WGYGEAKQPWKAE-----------ELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNVDR 2044
            TVR  T L+  L    +  EA  +   + E G KP + TYT L+ +L    +++    L+ ++ E G+ P+ I +NA+IN + + G +++A+ + + M+     P T T+N L+KGY   N+ KA     +L  ML+   V P+  TYN LI   C   N + A+ ++  M   G+ PD  TY ++  +  ++     A  +   ++   V PNV     ++ GYCK G + EA   + +M      PN + FN+L+ G       +G  +  TL+EE     G++P V T + +++     G  D     F  M+ +G +PD H ++   + Y R G   +AE ++  M + GV P++  ++++I G+   G+ N A  V ++M + G  P+  T+ +LI       +G  +  +P               ELL+ M E  ++    + + +      +G L  A ++  ++ R
Sbjct:  322 TVRTYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKSNVHKA---MGVLNKMLER-KVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGLC----ADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLLKDGDFDHAYSRFQQMLSSGTKPDAHTYTTFIQTYCREGRLLDAEDMMAKMRENGVSPDLFTYSSLIKGYGDLGQTNFAFDVLKRMRDTGCEPSQHTFLSLIKHLLEMKYGKQKGSEPELCAMSNMMEFDTVVELLEKMVEHSVTPNAKSYEKLILGICEVGNLRVAEKVFDHMQR 788          

HSP 3 Score: 166.007 bits (419), Expect = 8.118e-41
Identity = 91/375 (24.27%), Postives = 190/375 (50.67%), Query Frame = -2

Query:  698 FNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNV 1822
            +N ++N+ +  G V E  +++ +M +    P   T+N ++ GY  +G  EE+++ +  +++ G + P+  TY  LI  +C + +++ A+ V + M   G   + V Y  +            A  +  +M++++  P VRT  +++   C     +EAL  V  M+E G++PN+  +  L+             E L  M E G+ P+VIT++ ++N +   G+++   ++ + M    + P+   ++ L KGY ++    +A  +LN M +  V P+VV + ++I G C +G  + A R+   M + G+ P+  TY ++I    ++K+  +A +L   +E+KG++        + D +   G ++EA  +++ +
Sbjct:  186 YNTLLNSLARFGLVDEMKQVYMEMLEDKVCPNIYTYNKMVNGYCKLGNVEEANQYVSKIVEAG-LDPDFFTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKSN-VHKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKM 558          

HSP 4 Score: 137.117 bits (344), Expect = 1.220e-31
Identity = 79/310 (25.48%), Postives = 160/310 (51.61%), Query Frame = -2

Query:  698 ILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAE-RSTIQLV-----ADAWRAIGLLNEA--RRIIKNV 1603
            + +   C K+N +E + + ++++       +  YNT+  + A+ G     +Q+  EM  +KV PN+ T   +V GYCK GN+ EA ++V ++ E G+ P+   + SL+ G+    D +   +    M   G + + + ++ +++    A  +D+  ++F  M      P V  +++L K    +    EA +L+  M + G++PN+  +T +I   C+  K   A  +  +M E G+ PN+ TY  LI GY +      A +++++ME + +S   R+  +L+     ++  +A+G+LN+   R+++ +V
Sbjct:  160 LYVLDLCRKMNKDERFELKYKLI-------IGCYNTLLNSLARFGLVDEMKQVYMEMLEDKVCPNIYTYNKMVNGYCKLGNVEEANQYVSKIVEAGLDPDFFTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKSNVHKAMGVLNKMLERKVLPDV 462          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy TrEMBL
Match: F6H2Y7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0008g03850 PE=4 SV=1)

HSP 1 Score: 775.007 bits (2000), Expect = 0.000e+0
Identity = 382/630 (60.63%), Postives = 477/630 (75.71%), Query Frame = -2

Query:  374 RSIAMGKHQEGTI-MXXXXXXXXXXXXXXVDEKEQSHPGTGSADSEVSSKCIVCSGNNSCQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRI---IKNVDRGIASNTKDDIPAEGMEKTYKKQSLSASYSNVLQMPGVAMNEQNGSSSSKARSQMVLKKYDSS---------SLFPAQASVFGVQPGIICQKQLTTTREYRMHGQFVNSCKLVFIN 2224
             S A  +HQ+GT  + K+ V+A    V   D K + HP + SAD +   +CI C    SC+TVR+RTKLMNI+IEKG+PQE Q I +S+ E GHKP+LVTYT L++ALT  K + SI S+IS+VEENGM+PDSI FNA+INAFSESGN++EAMK F KMK+SG KPTTSTFNTL+KGYG  G+ EES KLLELM QD NV+PN RTYN+L+RAWCNK NI +AWNVV++MVASG+ PD VTYNTIA AYAQ GE  +AE +I EMQN+ V PN RTC II+ GYCKEG + EAL+FVYRMK+LG+QPNLVVFNSL+KGF+D  D +GV+E LTLMEEFGVKPDVITFSTIMNAWS+AG MDKC+EIFDDMVKA I+PD H +SILAKGY+RAGEP +AE +LN+M K G  PNVVIFTT+I+GWC+AG+M +A +++EKMCE G+ PNL T+ETLIWGYGEA+QPWK+EE+LQIMEE  +  E++T+ LVA+AWRA GL  EA RI   +KN +    ++ +D IP   +EK Y+K   SAS+ N LQ+P + +++Q GS S+  RS+MVL   D S         SL    +  FG +  IICQKQ  +  +  M+ Q  +SC LVF+N
Sbjct:   10 HSDAPTEHQDGTTDVKKEEVEALQTEVKRRDGKHELHPVSQSADPQSQVRCISCLSKTSCRTVRSRTKLMNIMIEKGRPQEVQSILDSIIEGGHKPSLVTYTTLLAALTIQKHFDSIHSIISQVEENGMEPDSIFFNAVINAFSESGNMQEAMKYFWKMKESGSKPTTSTFNTLIKGYGIAGEPEESQKLLELMSQDENVRPNLRTYNVLVRAWCNKKNIMKAWNVVYKMVASGLQPDAVTYNTIATAYAQNGEASQAEGIILEMQNSNVQPNERTCCIIIGGYCKEGKIKEALQFVYRMKDLGLQPNLVVFNSLIKGFIDAVDRDGVNEVLTLMEEFGVKPDVITFSTIMNAWSAAGFMDKCREIFDDMVKARIQPDAHVYSILAKGYVRAGEPEKAEEILNAMIKSGFHPNVVIFTTIINGWCSAGRMEYAIKIFEKMCECGIAPNLKTFETLIWGYGEARQPWKSEEVLQIMEEFNVQPEKTTLLLVAEAWRATGLKKEANRILSAVKNEEMTHETDREDKIPVGSLEKHYQKP--SASFPNQLQLPNLVISDQKGSGSATKRSRMVLGDTDFSLESSWTATRSLSLTHSCTFGARSPIICQKQ--SHGQLCMYSQLAHSCPLVFLN 635          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy TrEMBL
Match: A5BLD2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033966 PE=4 SV=1)

HSP 1 Score: 770.77 bits (1989), Expect = 0.000e+0
Identity = 380/630 (60.32%), Postives = 475/630 (75.40%), Query Frame = -2

Query:  374 RSIAMGKHQEGTI-MXXXXXXXXXXXXXXVDEKEQSHPGTGSADSEVSSKCIVCSGNNSCQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRI---IKNVDRGIASNTKDDIPAEGMEKTYKKQSLSASYSNVLQMPGVAMNEQNGSSSSKARSQMVLKKYDSS---------SLFPAQASVFGVQPGIICQKQLTTTREYRMHGQFVNSCKLVFIN 2224
             S A  +HQ+GT    K+ V+A    V   D K + HP + SAD +   +CI C    SC+TVR+RTKLMNI+IEKG+PQE Q I +S+ E GHKP+LVTYT L++ALT  K + SI S+IS+VEENGM+PDSI FNA+INAFSESGN++EAMK F KMK+SG KPTTSTFNTL+KGYG  G+ EES KLL+LM QD NV+PN RTYN+L+RAWCNK NI +AWNVV++MVASG+ PD VTYNTIA AYAQ GE  +AE +I EMQN+ V PN RTC II+ GYCKEG + EAL+FVYRMK+LG+QPNLVVFNSL+KGF+D  D +GV+E LTLMEEFGVKPDVITFSTIMNAWS+AG MDKC+EIFDDMVKA I+PD H +SILAKGY+RAGEP +AE +LN+M K G  PNVV+FTT+I+GWC+AG+M +A +++EKMCE G+ PNL T+ETLIWGYGEA+QPWK+EE+LQIMEE  +  E++T+ LVA+AWRA GL  EA RI   +KN +    ++ +D IP    EK Y+K   SAS+ N LQ+P + +++Q GS S+  RS+MVL   D S         SL    +  FG +  IICQKQ  +  +  M+ Q  +SC LVF+N
Sbjct:   10 HSDAPTEHQDGTTDAKKEEVEALQTEVKRRDGKHELHPVSQSADPQSQVRCISCLSKTSCRTVRSRTKLMNIMIEKGRPQEVQSILDSIIEGGHKPSLVTYTTLLAALTIQKHFDSIHSIISQVEENGMEPDSIFFNAVINAFSESGNMQEAMKYFWKMKESGSKPTTSTFNTLIKGYGIAGEPEESQKLLZLMSQDENVRPNLRTYNVLVRAWCNKXNIMKAWNVVYKMVASGLQPDAVTYNTIATAYAQNGEASQAEGIILEMQNSNVQPNERTCCIIIGGYCKEGKIKEALQFVYRMKDLGLQPNLVVFNSLIKGFIDAVDRDGVNEVLTLMEEFGVKPDVITFSTIMNAWSAAGFMDKCREIFDDMVKARIQPDAHVYSILAKGYVRAGEPEKAEEILNAMIKSGFHPNVVMFTTIINGWCSAGRMEYAIKIFEKMCECGIAPNLKTFETLIWGYGEARQPWKSEEVLQIMEEFNVQPEKTTLLLVAEAWRATGLKKEANRILSAVKNEETTHETDREDKIPVGSSEKHYQKP--SASFPNQLQLPNLVISDQKGSGSATKRSRMVLGDTDFSLESSWTATRSLSLTHSCTFGARSPIICQKQ--SHGQLCMYSQLAHSCPLVFLN 635          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy TrEMBL
Match: A0A067KR04_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07785 PE=4 SV=1)

HSP 1 Score: 767.689 bits (1981), Expect = 0.000e+0
Identity = 368/581 (63.34%), Postives = 452/581 (77.80%), Query Frame = -2

Query:  374 CIVCSGNNSCQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNVDRGIASNTKD----DIPAEGMEKTYKKQSLSASYSNVLQMPGVAMNEQNGSSSSKARSQMVLKKYDSS---------SLFPAQASVFGVQPGIICQKQLTTTREYRMHGQFVNSCKLVFIN 2077
            C+ C     C+TVR+RTKLMNILIEKGKPQEA+ IF+SL E GHKP+L+TYT L++ALT  KR+ SI S+IS+VEENGMKPDSI FNA+INAFSESGN++EAM  F KMK++G KPTTST+NTL+KGYG  GK EES+KLL+LM ++G+V+PN RTYN+L+RAWCNK NI EAWNVVH+MVAS M PDVVTYNTIA AY Q GET  AE +I EMQN  + PN RTCGII++GYCKEG + EALRFVYRMKE GV PNLVV NSL+KGF++  D +GVDE L LMEEF VKPDVITFSTIMNAWS+AG MDKC+EIFDDMVKAGIEPD HA+SILAKGY+RA EP +AE LL +M + G  PNVVIFTTVISGWC++GKM +A RV++ MCE G++PNL T+ETL+WG+ EAKQPWKAEE+LQIM E  +  ERST+ LVA+AWRAIGL  EA RI+  + R   ++ KD    D+P E +EK Y+KQS + SYSN+L +P    N+Q GSS++  + +MVL++ + S         S+  +Q   FG +  IIC KQ  +  ++ M+GQF  SC  VF+N
Sbjct:    9 CMFCMTKEGCRTVRSRTKLMNILIEKGKPQEAESIFDSLIEGGHKPSLITYTTLLAALTMQKRFNSIHSIISQVEENGMKPDSIFFNAVINAFSESGNMEEAMDTFWKMKENGMKPTTSTYNTLIKGYGISGKPEESAKLLDLMSREGDVKPNLRTYNVLVRAWCNKKNISEAWNVVHKMVASRMQPDVVTYNTIATAYTQKGETNLAEGIISEMQNKGMQPNERTCGIIISGYCKEGRIKEALRFVYRMKEFGVYPNLVVLNSLIKGFIETMDRDGVDEVLKLMEEFNVKPDVITFSTIMNAWSTAGFMDKCREIFDDMVKAGIEPDAHAYSILAKGYVRAQEPEKAEELLTTMIRSGFHPNVVIFTTVISGWCSSGKMEYAIRVFDMMCEYGISPNLKTFETLMWGFSEAKQPWKAEEILQIMREFKVEPERSTVLLVAEAWRAIGLTKEANRILGTIKRKEMTHRKDRAEEDMPVESLEKLYQKQSTNVSYSNLLHVPSTVTNDQKGSSAALRKGRMVLREAEFSMEYSWLAAKSMCFSQTCKFGSRLPIICHKQ--SQGQHGMYGQFAQSCTAVFLN 587          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy TrEMBL
Match: M1C967_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400024327 PE=4 SV=1)

HSP 1 Score: 759.214 bits (1959), Expect = 0.000e+0
Identity = 370/586 (63.14%), Postives = 453/586 (77.30%), Query Frame = -2

Query:  374 VSSKCIVCSGN-NSCQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVYRMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKNV---DRGIASNTKDDIPAE-GMEKTYKKQSLSASYSNVLQMPGVAMNEQNGSSSSKARSQMVLKKYDS---------SSLFPAQASVFGVQPGIICQKQLTTTREYRMHGQFVNSCKLVFIN 2089
            +SS+C  C G+ +SCQ VR+RTKLMNILIE+ KPQE   IF  LTE+GH+PTLVTYT +++A T  KR+KSI  L+SK+E++G+KPDSI FNAMINAFSESGN+KEAMK+F KM+   CKPTTSTFNTL+KG+G IGK EES KLLE+M    NV+PNDRTYNIL+RAWCNK  I EAWNVVHRMV++G+ PDVVTYNTIA AYA+  E  RAE MIF+MQN+KVAPN RTCGIIV GYCKEGNMTEALRFVYRMK+LGV PNLV+FNSL+KGFLD+TDT+GVDE LTLMEEFGVKPDV+TFSTIMNAWSSAGLMDKCQEIFDDM+KA IEPD HAFSILAKGY+RAGEP +AE+LL  M   GV PNVV+FTT+ISGWC+AG+M +A RV+E+MC+  V+PNL T+ETLIWGYGEAKQPWKAEE LQ+M++ G++  ++T++LV+DAWRAIG LNEA++I  +V   D    +  +D  P +   E+ Y   SLS+            ++ +NGSS    RSQ+VL +Y S          S+       F V+  +I  KQ     +  ++G FVNSC++V +N
Sbjct:   42 ISSRCTTCWGSMSSCQIVRSRTKLMNILIERKKPQEVHSIFQGLTEDGHRPTLVTYTTVLAAFTLQKRFKSIPLLLSKLEQDGLKPDSIFFNAMINAFSESGNLKEAMKVFHKMRDKQCKPTTSTFNTLIKGFGIIGKPEESLKLLEIMSLQENVKPNDRTYNILVRAWCNKSKIAEAWNVVHRMVSAGVKPDVVTYNTIATAYARNKEPGRAEAMIFQMQNHKVAPNHRTCGIIVDGYCKEGNMTEALRFVYRMKDLGVLPNLVIFNSLIKGFLDITDTSGVDETLTLMEEFGVKPDVVTFSTIMNAWSSAGLMDKCQEIFDDMIKASIEPDSHAFSILAKGYVRAGEPEKAEALLKVMASSGVHPNVVMFTTIISGWCSAGQMEYAMRVFERMCKRDVSPNLKTFETLIWGYGEAKQPWKAEEFLQVMDKMGVTHTKNTLRLVSDAWRAIGFLNEAKKISGDVEYDDSEFLNALEDKQPGDNNFERVYPNDSLSSLKQ---------VSNKNGSSPVNKRSQIVLSRYSSFAGSINVATKSIMLPHTCGFKVKSLMIFDKQCQV--QIGIYG-FVNSCRMVALN 615          

BLAST of g.barbadense_cottongen_reftransV1_0097431 vs. ExPASy TrEMBL
Match: V7AQD2_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_010G165600g PE=4 SV=1)

HSP 1 Score: 756.903 bits (1953), Expect = 0.000e+0
Identity = 366/583 (62.78%), Postives = 459/583 (78.73%), Query Frame = -2

Query:  377 SKCIVCSGNNSCQTVRARTKLMNILIEKGKPQEAQFIFNSLTEEGHKPTLVTYTALVSALTRLKRYKSIISLISKVEENGMKPDSILFNAMINAFSESGNVKEAMKIFQKMKKSGCKPTTSTFNTLMKGYGNIGKAEESSKLLELMLQDGNVQPNDRTYNILIRAWCNKLNIEEAWNVVHRMVASGMHPDVVTYNTIARAYAQTGETYRAEQMIFEMQNNKVAPNVRTCGIIVTGYCKEGNMTEALRFVY-RMKELGVQPNLVVFNSLVKGFLDVTDTNGVDEALTLMEEFGVKPDVITFSTIMNAWSSAGLMDKCQEIFDDMVKAGIEPDVHAFSILAKGYIRAGEPGEAESLLNSMGKFGVQPNVVIFTTVISGWCTAGKMNHAARVYEKMCEIGVTPNLATYETLIWGYGEAKQPWKAEELLQIMEEKGISAERSTIQLVADAWRAIGLLNEARRIIKN-------VDRGIASNTKDDIPAEGMEKTYKKQSLSASYSNVLQMPGVAMNEQNGSSSSKARSQMVLKKYD-----SSSLFPAQAS-VFGVQPGIICQKQLTTTREYRMHGQFVNSCKLVFI 2083
            S C++C G NSCQTV +RTKLMN LI KGKP EAQ +FN+LTEEGHKPTL+TYT LV+ALTR KR KSI ++++KV +NGMKPDSIL NAMINAFS+SG V EAMKIF+KMK+ GCKPTTST+NTL+KG+G  G+  E+ KL E+M QD NV+PNDRTYNILI+AWC K  +EEAWNV+H+MVASG+ PDVVTYNT+ARAYAQ GET +AE++I +MQ NKV PN RTCGII++GYCKEGNMTEALRF+Y +MKELGV PN VVFNSL+KG+LD+ DT+GVDEAL LMEEFG+KPDV+TFSTIMNAWSSAGLMD C+EIF++MVKA IEPD+HA+SILAKGY RAG+  +AE+LL SM K+GVQPNVVI TT+ISGWC  GKM+ A R+YEKM ++G+ PNL TYETLIWGYGEAK PWKAEELL  MEE+G++ E ST+QLVADAWR+IGLL EA RI+ +       +D+   S   D+I        Y +Q  SAS+SN+LQ+P V +     +++   RSQ V+K  D     ++S+F  + +  +GVQ   + ++Q+    + ++   F++ C++V I
Sbjct:   42 SHCVICRGGNSCQTVLSRTKLMNTLIGKGKPHEAQTVFNNLTEEGHKPTLITYTTLVAALTRQKRLKSIPAVLAKVADNGMKPDSILINAMINAFSDSGKVDEAMKIFKKMKEYGCKPTTSTYNTLIKGFGIAGRPYEAMKLSEIMGQDQNVKPNDRTYNILIQAWCTKNKLEEAWNVMHKMVASGIQPDVVTYNTMARAYAQIGETEKAERLILKMQYNKVKPNERTCGIIISGYCKEGNMTEALRFLYIKMKELGVHPNPVVFNSLIKGYLDIKDTDGVDEALALMEEFGIKPDVVTFSTIMNAWSSAGLMDNCEEIFNNMVKADIEPDIHAYSILAKGYARAGQSQKAEALLTSMTKYGVQPNVVILTTIISGWCAEGKMDRAIRLYEKMHDVGIPPNLKTYETLIWGYGEAKLPWKAEELLTTMEERGVAPETSTVQLVADAWRSIGLLKEANRILNDSEEEESELDKKFDS---DNI--------YTRQKFSASHSNMLQIPDVVVPHPERTTNGNIRSQRVVKVSDNMRHITTSMFLVRTTNSYGVQAHSVSKQQI----QNQVVRPFLDYCRVVSI 609