g.barbadense_cottongen_reftransV1_0097430, g.barbadense_cottongen_reftransV1_0097430 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y005_SYNY3 (Uncharacterized protein sll0005 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=sll0005 PE=3 SV=1)

HSP 1 Score: 263.077 bits (671), Expect = 1.025e-72
Identity = 149/442 (33.71%), Postives = 241/442 (54.52%), Query Frame = -3

Query: 2490 LPEA--YDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILR-LGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSL 3806
             PE+  Y  + + +++  RP  V  RL+ +        + I    L+           D  +S+    + L+E + +LGPT+IKVGQ+LSTRPD++ P     L+ L DQ+P FP  +A + IEEEL +P    ++ +S EP+AAAS GQVY+G    G  VAVKVQRP+L   +  D+YI+R L L   + + + +SD     DEL   +  E++Y  EA N  +F   +     + VP ++   T +R LTMEW+ G                   K ++ Q +DA      LV  GV+ SL QLLE G  HADPHPGNL   A GR+ +LDFG++  ++   ++ ++ ++VH+VN D+ SL +   ++D ++P T+++ +   L    G   L   + ++ F  +  ++ ++  ++ FR+P YY L++RS+ +LEG ++
Sbjct:   47 FPESWEYHPDLIMEFYRKRPLQVLGRLINILFPLLRFILGIWWEKLRGK---------DPTVSRAK-AIQLRELLTNLGPTYIKVGQALSTRPDLVPPVFLDELTTLQDQLPSFPNEVAYRFIEEELGAPAEEIYAELSPEPIAAASLGQVYKGKLKTGEAVAVKVQRPDLVRRITLDIYIMRSLSLWARRSVKRLRSDLVAITDELASRVFEEMNYYQEAINGEKFAQLYGSLPEIYVPSIYWQYTGRRVLTMEWVEG-------------IKLTNIKAIQAQGIDATH----LVEVGVQCSLRQLLEHGFFHADPHPGNLLAMADGRLAYLDFGMMSTIQPYQRYGLIEAVVHLVNRDFDSLAKDYVKLDFLKPDTDLKPIIPALGQVFGNA-LGASVAELNFKSITDQMSAMMYEFPFRVPAYYALIIRSMVTLEGIAI 460          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y1919_SYNY3 (Uncharacterized protein slr1919 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=slr1919 PE=3 SV=1)

HSP 1 Score: 248.054 bits (632), Expect = 1.620e-68
Identity = 154/460 (33.48%), Postives = 236/460 (51.30%), Query Frame = -3

Query: 2484 KPLPEA--------YDAEEVADYFSHRPHVVAFRLLEV---FSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAK-----RKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAK-RRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLCF 3812
            KPLP          Y+A+ +A+Y+  RP  V +R LEV   F  F +  +  +  G  +  +   ++D             L+E +  LGPTFIKVGQ+LSTRPD++  +  + L +L DQ+PPF   LA +++EE+L   +   +  IS  PVAAAS GQVYR     G +VAVKVQRPNLR  +  D+Y++RLG    QK  +        D  L  DE G  L  E+DY  E  NA +F +     + ++VP ++   + ++ LT+EW+                   G K  +  K+ A      D++  GV + L QLLE G  HADPHPGNL  T  GR+ ++DFG++ ++E   +  + +SIV ++N D+ +L +    +  + P T+I  +   LE+  G   +   + D  F  +      +   Y FR+P  + L++RSL + EG +L  
Sbjct:    5 KPLPPQSELDNIRRYNAQAIANYYRRRPWKVLWRALEVVWSFGFFLTCLLWDQWTGQVEYYKRQRAED-------------LRELLTKLGPTFIKVGQALSTRPDLVRRDFLEELIKLQDQLPPFDNDLAFQLMEEQLGMKVDEAYREISAHPVAAASLGQVYRAMLFSGEEVAVKVQRPNLRPRLSLDLYLMRLG---AQKFGRFLPLNLGHDLTLIVDEFGIKLFEEIDYLNEGRNAEKFAENFHGDAEVKVPCIYWQYSNQKVLTLEWI------------------QGFKLTDTDKIRAAGLDPSDIIRIGVTSGLRQLLEHGFFHADPHPGNLFATLDGRMAYIDFGMMDQLEPGTKETIASSIVQLINKDYLALTEDFIALGFLAPNTDITPIIPALENVFGSA-IGQSVQDFNFKTITDDFSELMYDYPFRVPAKFALIIRSLVTQEGLALSL 429          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y1796_ARATH (Uncharacterized aarF domain-containing protein kinase At1g79600, chloroplastic OS=Arabidopsis thaliana GN=At1g79600 PE=2 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 1.937e-66
Identity = 147/439 (33.49%), Postives = 237/439 (53.99%), Query Frame = -3

Query: 2490 YDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRG-CTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLL--QKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKR-RLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSL 3794
            Y  E +A     +P     R LE+  +    A+++   G+ +        ++++N+ +    + L+     LGPTF+K+GQ LSTRPD+  P+  + L+EL D +P FP   A   IE ELD  + + FS +S EP+AAAS GQVY+      G  VAVKVQRP +   +  D Y++R G+G L  + +    +D     DE    +  EL+Y  EA NA +F   ++  + + VP +F   T ++ LTMEW+                   G+K  E+  ++++  ++LDLVN G++ SL QLLE G  HADPHPGNL  T  G++ FLDFG++    ++ +FA++  +VH+VN D+ ++ +    +  + P  ++  +   L D   +  L   + ++ F  ++  + +V  +Y F +PPYY L+LRSL  LEG +L
Sbjct:   96 YSPELIARKHGSQPFKALRRSLEILGALGGFALKL---GIDQK-----QGNLEKNMKKR--AIELRRIFTRLGPTFVKLGQGLSTRPDLCPPDYLEELAELQDALPTFPDAEAFACIERELDLSLETIFSSVSPEPIAAASLGQVYKAQLRYSGQVVAVKVQRPGIEEAIGLDFYLIR-GVGKLINKYVDFITTDVLTLIDEFACRVYQELNYVQEAQNARRFKKLYADKADVLVPDIFWDYTSRKVLTMEWV------------------EGTKLNEQLAIESQGLKVLDLVNTGIQCSLRQLLEYGFFHADPHPGNLLATPDGKLAFLDFGMMSETPEEARFAIIGHVVHLVNRDYEAMARDYYALKFLSPDVDVTPIIPALRDFFDDA-LNYTVSELNFKTLVDGLGAVFYQYPFNVPPYYALILRSLTVLEGLAL 504          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y4139_ARATH (Uncharacterized aarF domain-containing protein kinase At4g31390, chloroplastic OS=Arabidopsis thaliana GN=At4g31390 PE=2 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 2.721e-64
Identity = 134/377 (35.54%), Postives = 204/377 (54.11%), Query Frame = -3

Query: 2484 LKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCT-LDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRK--SDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKV-DAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLCF 3602
            L+  + +LGP+FIK GQ L+ RPDII  +    L  L D +PPFP  +A  IIEEEL  P+ + FS IS + +AAAS GQVYR      G DVA+KVQRP +  ++ RD+++ R     L   + +K   +  L  DE G+ L+ ELDYTLEA N   FL+       +++P V+++L   R L MEW+                   G +  + Q + DA   L   +  GV A+L QLLE G+ H DPHPGN+     GRI ++DFG +  + ++++  ++ ++VH VN D+  +    T +  +   T++  +   LE A+ +     G+ D  F  V G+   +   +  R+P  ++LV+RSL + EG  +CF
Sbjct:  170 LRNLLCNLGPSFIKAGQVLANRPDIIREDYMNELCILQDDVPPFPNEVAFNIIEEELGQPLENIFSKISSQTIAAASLGQVYRATLRATGEDVAIKVQRPQIEPIIYRDLFLFRTLASFLNGFSLQKLGCNAELIVDEFGEKLLEELDYTLEARNIEDFLENFKDDPTVKIPGVYKNLCGPRVLVMEWI------------------DGIRCTDPQAIKDAGIDLNGFLTVGVSAALRQLLEFGLFHGDPHPGNIFAMQDGRIAYVDFGNVAVLSQQNKQILIDAVVHAVNEDYGEMANDFTRLGFLAKDTDVSPIVPALE-AIWQNSAGKGLADFNFRSVTGQFNKLVYDFPIRIPERFSLVIRSLLTQEG--ICF 525          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y1181_ARATH (Uncharacterized aarF domain-containing protein kinase At1g71810, chloroplastic OS=Arabidopsis thaliana GN=At1g71810 PE=2 SV=1)

HSP 1 Score: 233.802 bits (595), Expect = 1.151e-62
Identity = 135/372 (36.29%), Postives = 197/372 (52.96%), Query Frame = -3

Query: 2490 LKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRG-CTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSL 3602
            L++ ++ LGP ++K+ Q++S+RPD+I P     LS L DQI PF   +A  +IE+EL  PI   FS IS EPVAAAS GQVY+      G  VAVKVQRP +R  +  D  ILR   GL++K  +  SD     DE    L  E+DY  EA N  +F   +     + VPK++   +  + L MEW+ G                        QK++    L  LV  GV  S  QLLE G  HADPHPGN   T  G++ +LDFG++     + +   + + +H+VN D+ +L +    + ++ P      VT  L D   +  +  G+ +I F  +LG +     ++ FR+PPY++LV+RSLA LEG ++
Sbjct:  123 LRKLLVELGPAYVKIAQAVSSRPDLIPPIYLDELSLLQDQITPFSTEVAFNMIEDELGLPIDELFSEISPEPVAAASLGQVYQARLRRSGKVVAVKVQRPGVRAAIALDTLILRYIAGLIKKAGRFNSDLEAVVDEWATSLFKEMDYLNEAQNGIKFRKLYGGIKDVLVPKMYTEYSTSKVLVMEWVEG------------------------QKLNEVNDLY-LVEVGVYCSFNQLLEYGFYHADPHPGNFLRTYDGQLAYLDFGMMGDFRPELRDGFMEACLHLVNRDFKALAKDFVTLGLLPPTAEKSAVTKALTDVFQDA-ISRGVRNISFGDLLGDLGKTMYRFKFRIPPYFSLVIRSLAVLEGIAI 468          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y095_SYNY3 (Uncharacterized protein sll0095 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=sll0095 PE=3 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 6.174e-48
Identity = 125/387 (32.30%), Postives = 200/387 (51.68%), Query Frame = -3

Query: 2499 QYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGL-------LQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLV-NKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTN---IRRVTMDLEDALGEVELKDGIPDIK-FSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 3623
            +Y     L + +L LGPTFIK+GQSLSTR DII  E  +A ++L D++PPF    A+ +IE+EL   I   F      P+A+AS GQV+R     G  V VKVQRP L  ++  D  +L   L L        +++A++     +Y  E    L  E+DY  E  NA +F    + +  ++VP+++   T +  LT+E++                   G K  +RQ ++     LDLV   G+ A L QLL  G   +DPHPGN+   + G + F DFG +  ++   +  M+ +   ++  D + +L+AL  M +V P  +   I+R+   L D       +D   DIK F +V  +++++  +  FR+PP  T +L+S+++L+G
Sbjct:   54 RYRRAQWLVQQLLYLGPTFIKIGQSLSTRADIIPAEYIEAFTQLQDRVPPFDSRQAIAVIEQELHGAIDEIFQQFEVTPLASASLGQVHRAVLPTGEAVVVKVQRPGLDSLLNLDFELLHQTLRLAKRWLPGFRRLAQKYEIEAIY-QEFFSLLFLEIDYIHEGKNAERFRQNFADYPRVRVPEIYWQYTTRMVLTLEYLP------------------GIKVDDRQALETAGINLDLVIQTGICAYLKQLLVDGFFQSDPHPGNMAVDSQGDLIFYDFGTMAEVKIIAKDQMVQTFFAVLRKDTNQVLEALIYMGLVEPKGDLSPIKRIINFLLD-----NFRDKPIDIKAFDQVGEEVYAMFQQQPFRLPPQMTFILKSISTLDG 416          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: UBIB_NEIMB (Probable protein kinase UbiB OS=Neisseria meningitidis serogroup B (strain MC58) GN=ubiB PE=3 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 3.226e-44
Identity = 131/413 (31.72%), Postives = 197/413 (47.70%), Query Frame = -3

Query: 2499 RIRMAGLKKSLR------------PGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAK--RKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 3695
            R R+AGL  SL             P SSK     L      + L+  + SLGP FIK GQ LSTRPD+I  + +  L++L D++PPF   L+ + IE+ L   I   ++    EP+A+AS  QV++     G  VAVKV RPNL  V+ +D+ ++R G G ++++    ++  PR    E  K L  ELD   EAANASQ      +   + VPKVF        LT+EWM G   +D+               L+   +D    L  L + GVE   TQ+   G  HAD HPGN+   A  R   LDFG++  +    +  +  + +   N D+  +  A  E   V   T    +   +  A+ E      I  I F  VL +++ V+ +++  + P   L+ ++L ++EG
Sbjct:   16 RYRLAGLCASLMGSGWICALLKMMPQSSK-----LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLSREQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGEQVAVKVLRPNLLPVIEQDLSLMRFGAGWVERLFADGKRLKPREVVAEFDKYLHDELDLMREAANASQLGRNFQNSDMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAK-------------LKADGID----LHKLADYGVEIFFTQVFRDGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATAHIESGWVPADTRAEELEAAVR-AVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQLVLLQKTLLNIEG 405          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y1770_SYNY3 (Uncharacterized protein sll1770 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=sll1770 PE=3 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 2.126e-42
Identity = 116/374 (31.02%), Postives = 180/374 (48.13%), Query Frame = -3

Query: 2499 LKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAK--RKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYT-ASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTN---IRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 3602
            ++E +LSLGPTFIKVGQ  STR D+   E  + LS+L D++P F    A  IIEEEL  PI   +      P+AAAS GQV++     G DV VKVQRP L+ +   D+ IL+      Q   K  R  D     +E  K L  E DY  E  +A  F        +++VP+V+   T  + LT+E++ G                  S Y   +    +R+  +L   G  A L QLL  G  HADPHPGNL  +  +G + F DFG++  +    +  ++ ++  +   +   ++ +L  +  ++   +   IRR    L D      +     +   +++   ++ +A    FR P  +T V+R+ ++LEG
Sbjct:   88 IRENLLSLGPTFIKVGQLFSTRSDLFPAEYVEELSKLQDEVPAFSYEQAAGIIEEELGKPIAKLYRSFDPVPLAAASLGQVHKAQLHTGEDVVVKVQRPGLKKLFTIDLAILKKIAQYFQNHPKWGRGRDWNGIYEECCKILWQETDYLREGRSADTFRRNFRGEDWVKVPRVYWRYTSTQILTLEYLPGIKI---------------SHYDALEAAGLERK--ELAQLGARAYLFQLLNHGFFHADPHPGNLAVSPEAGELIFYDFGMMGEITPDTKNKLMDTLFGVAEKNAERIVNSLVALGALKETEDMGPIRRSVQFLLDNF----MDKPFEEQSITKISDDLYEIAYDQPFRFPATFTFVMRAFSTLEG 440          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: UBIB_NEIMA (Probable protein kinase UbiB OS=Neisseria meningitidis serogroup A / serotype 4A (strain Z2491) GN=ubiB PE=3 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 7.316e-42
Identity = 128/413 (30.99%), Postives = 194/413 (46.97%), Query Frame = -3

Query: 2499 RIRMAGLKKSLR------------PGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAK--RKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 3695
            R R+AGL  SL             P SSK     L      + L+  + SLGP FIK GQ LSTRPD+I  + +  L++L D++PPF   L+ + IE+ L   I   ++    EP+A+AS  QV++     G  VAVKV RPNL  ++ +D+ ++R G   ++++    R+  P     E  K L  ELD   EAANA Q      + + + VPKVF        LT+EWM G    D+               L+   +D    L  L + GVE   TQ+   G  HAD HPGN+   A  R   LDFG++  +    +  +  + +   N D+  +  A  E   V   T    +   +  A+ E      I  I F  VL +++ V+ +++  + P   L+ ++L ++EG
Sbjct:   16 RYRLAGLCASLMGSGWICALLKMMPQSSK-----LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLSREQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQDLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFHNSNMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAK-------------LKADGID----LHKLADYGVEIFFTQVFRDGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATAHIESGWVPADTRAEELEAAVR-AVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQLVLLQKTLLNIEG 405          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y889_SYNY3 (Uncharacterized protein slr0889 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=slr0889 PE=3 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 1.262e-40
Identity = 111/375 (29.60%), Postives = 181/375 (48.27%), Query Frame = -3

Query: 2499 VLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIA-KRKSDPRLYADELGKGLVGELDYTLEAA---NASQFLDAHSHFSFMQ--VPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 3605
            VL + ++ LGP +IK+GQ LSTRPD++ P    AL+ L   +PP P      +++ E   P+G  F  I  EP+AA S GQ++R     G  VA+KV+RP +  +V +D  +++    LL      +  D    ADE  + +  EL++  EAA   N    L   + F   Q  +PKV+  LT ++ L +EW+ G            S         ++   + K+ +  L+ +   A   QL   G  HADPHPGN+ Y A GR+  +D G++ R++ + +  +   ++ IV+ D     Q   E+       N +R+ +D E  L +      + +  FS V+ +   +A     ++P    L  + LA+LEG
Sbjct:   45 VLTKILVELGPFYIKLGQLLSTRPDLLPPRYINALTALQSNVPPLPWSAIEDLLQREFPQPLGETFQEIESEPIAAGSIGQIHRAVLQSGETVAIKVKRPGIDVIVEQDSLLIKDVAELLALTEFGQNYDIVKLADEFTQTVKAELNFDTEAAYTNNLRTNLAKTTWFDPNQLVIPKVYWELTNQKFLVLEWLDGVPILTADLTQPPS---------DKDIAEKKKEITTLLFR---AFFQQLYVDGFFHADPHPGNIFYLADGRLALIDCGMVGRLDPRTRQLLTEMLLAIVDLDAKRCAQLTVELSESVGRVNFQRLEVDYERMLRKY-YDLSLSEFNFSEVVYEFLRIARVNKLKVPACLGLYAKCLANLEG 406          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: G7I9J4_MEDTR (AarF domain kinase OS=Medicago truncatula GN=MTR_1g095480 PE=4 SV=1)

HSP 1 Score: 800.431 bits (2066), Expect = 0.000e+0
Identity = 400/543 (73.66%), Postives = 454/543 (83.61%), Query Frame = -3

Query: 2487 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLC 4115
              NF HFG VVR+DM+FLK+G   GV WAN+ FRIPQ  K VDD+VWLRNLEDP  +     P WP+P YP LSG+DL+M DLKALEAY SYFY+ SK WSKPLPE YD ++VA YFS RPHVVA R+LEVFSSFASA + IR +GL+K L   +   +D+  S+YNFG+VLKETML+LGPTFIKVGQSLSTRPDIIG E+SKALSELHDQIPPFPR +AMKI+EEEL +P+ SFFSYISEEPVAAASFGQVY   T DG +VAVKVQRPNLRHVVVRD+YILRLGLGLLQKIAKRKSD RLYADELG+G VGELDYTLEAANA +F + HS FSFM+VPK+F HL+RKR LTMEWM GE      +   S S  + ++Y +RQKVDAKRRLLDLVNKGVEA+L QLLETG++HADPHPGNLR T+SG IGFLDFGLLC+MEK+HQFAMLASIVHIVNGDW+SL+ AL +MD+VRPGTNIR VTM+LE ALGEVE KDGIPD+KFSRVLGKI SVA KYHFRMP YYTLVLRSLAS EG ++ 
Sbjct:   33 LGNFGHFGQVVRKDMEFLKRGFNNGVAWANDAFRIPQIAKKVDDLVWLRNLEDPQ-ATSFSTPSWPEPWYPGLSGVDLLMYDLKALEAYASYFYHLSKIWSKPLPETYDPQDVAHYFSARPHVVALRMLEVFSSFASAGVSIRTSGLRKFLPINAEGGMDDKTSEYNFGLVLKETMLNLGPTFIKVGQSLSTRPDIIGVEMSKALSELHDQIPPFPRTVAMKILEEELGAPLESFFSYISEEPVAAASFGQVYFARTTDGVNVAVKVQRPNLRHVVVRDIYILRLGLGLLQKIAKRKSDLRLYADELGRGFVGELDYTLEAANALKFREVHSSFSFMRVPKIFLHLSRKRVLTMEWMVGE----SPTDLISVSTGNSTEYSDRQKVDAKRRLLDLVNKGVEATLVQLLETGLIHADPHPGNLRCTSSGEIGFLDFGLLCQMEKRHQFAMLASIVHIVNGDWASLVNALIDMDMVRPGTNIRLVTMELEQALGEVEFKDGIPDVKFSRVLGKILSVAFKYHFRMPAYYTLVLRSLASFEGLAIA 570          

HSP 2 Score: 231.106 bits (588), Expect = 3.374e-59
Identity = 124/218 (56.88%), Postives = 156/218 (71.56%), Query Frame = -1

Query:  482 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKR 1135
            V+LNRKKEF+W+RL+LF+RVGATR++LQLV ++S ETS D  P++  G FD+AYL+L +LPSKDGV LRRL+MTADGAS++RA VSKE K  R QLCK+IAD L Q M+K  GQ V  +QY  ++ LA G  N E        +SS  YDY S+  DRRL++I SK++ SA     LMLRF W S V  I ASALA HR+++SLS AY+GP    PKR
Sbjct:  604 VLLNRKKEFQWQRLSLFLRVGATRKALQLVTSNS-ETSPDQSPNKAAGTFDIAYLILTILPSKDGVALRRLLMTADGASIIRAMVSKEGKVIRQQLCKVIADALCQWMIKLCGQGVIDTQYP-RVMLANGTSNKES--GRSPRSSSPSYDYISIFRDRRLRVIFSKVVKSASSHKILMLRFCWSSLVIIITASALACHRVVLSLSEAYLGPIFDAPKR 817          

HSP 3 Score: 68.5514 bits (166), Expect = 3.504e-8
Identity = 32/37 (86.49%), Postives = 34/37 (91.89%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLA+AAD  FKTFEAAYP+VVRKLLTENSA TRKILH
Sbjct:  566 GLAIAADKKFKTFEAAYPYVVRKLLTENSAATRKILH 602          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: I1LD20_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 798.119 bits (2060), Expect = 0.000e+0
Identity = 402/543 (74.03%), Postives = 466/543 (85.82%), Query Frame = -3

Query: 2487 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLC 4115
              +FS F  VVR+D++FLK+G+  GV WA ETFRIP+  K +DDVVWLRNLED   SPP   P WPQPCYP L+G+DL+M DLKA EAY SYFYY SK W++PLP+ YD ++VA YFS RPH+V  R+LEV  SFA+A I IR +G  K LR    +D+D+  SQYNFGMVLKET+L+LGPTFIKVGQSLSTRPDIIG E+SKALSELHDQIPPFPR +AMKI+EEE   P+ SFFSYISEEP+AAASFGQVY   T DG++VAVKVQRPNL HVVVRD+YILRLGLGLLQKIAKRKSDPRLYADELGKG VGELDYTLEAANAS+FL+ HS F+FM VPKVF HLTRKR LTMEWM GES TDLLS+T  +S+ + S+Y ERQK+DAKRRLLDLV+KG+E++L QLLETG+LHADPHPGNLRYT+SG+IGFLDFGLLC+MEK+HQ AMLASI+HIVNGDW+SL++AL +MDVVRPGTNIR VT++LE ALGEVE K+GIPD+KFSRVLGKIW+VALK+HFRMPPYYTLVLRSLASLEG ++ 
Sbjct:   32 LGDFSLFAQVVRKDVEFLKRGIDNGVAWAKETFRIPEVAKKIDDVVWLRNLED-PTSPPLPSPSWPQPCYPGLTGVDLLMYDLKAFEAYASYFYYFSKLWTRPLPQDYDPQQVAQYFSVRPHLVTLRVLEVLFSFATAMISIRTSGFSKFLRLIPEEDVDDTSSQYNFGMVLKETLLNLGPTFIKVGQSLSTRPDIIGVEMSKALSELHDQIPPFPRTVAMKIMEEEFGCPLESFFSYISEEPIAAASFGQVYFARTTDGNNVAVKVQRPNLHHVVVRDIYILRLGLGLLQKIAKRKSDPRLYADELGKGFVGELDYTLEAANASKFLEVHSSFTFMNVPKVFPHLTRKRVLTMEWMVGESPTDLLSVTAGNSVGNVSEYSERQKLDAKRRLLDLVSKGIESTLVQLLETGLLHADPHPGNLRYTSSGQIGFLDFGLLCQMEKRHQLAMLASIIHIVNGDWASLVRALVDMDVVRPGTNIRLVTLELEQALGEVEFKEGIPDVKFSRVLGKIWTVALKHHFRMPPYYTLVLRSLASLEGLAIA 573          

HSP 2 Score: 231.876 bits (590), Expect = 2.060e-59
Identity = 124/225 (55.11%), Postives = 163/225 (72.44%), Query Frame = -1

Query:  467 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTE--LHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAISA 1135
            V+LN++KEF+W+RL+LF+RVGATR++L+LV AS+ ETSLD+  S+     D+AYL+LRLLPSKDGV +RRL+MTADGASL++A VSKE + FR QLCKII  ILYQ M+K  GQ +  +QYS ++ LA G  + E  L P + L T    YDY S+  DRRL++I SK+L SA ++  LMLRF W S    I AS LA H+L++SLS AY+      PKR+A+SA
Sbjct:  607 VLLNQRKEFQWQRLSLFLRVGATRKALRLV-ASNSETSLDHSTSKATDTIDIAYLVLRLLPSKDGVAIRRLLMTADGASLIKAMVSKEGEFFREQLCKIIVGILYQWMIKLFGQGITITQYS-RMVLANGPSSKESGLSPRSSLPT----YDYNSIFRDRRLRVIFSKVLKSASRDKILMLRFSWASLKIIITASTLACHQLVVSLSEAYLSKIFDAPKRYAVSA 825          

HSP 3 Score: 66.6254 bits (161), Expect = 1.212e-7
Identity = 31/37 (83.78%), Postives = 34/37 (91.89%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLA+AAD +FKTFEAAYP+VVRKLLTENSA TR ILH
Sbjct:  569 GLAIAADTNFKTFEAAYPYVVRKLLTENSAATRNILH 605          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: A0A072VPE2_MEDTR (AarF domain kinase OS=Medicago truncatula GN=MTR_1g095480 PE=4 SV=1)

HSP 1 Score: 797.734 bits (2059), Expect = 0.000e+0
Identity = 400/543 (73.66%), Postives = 454/543 (83.61%), Query Frame = -3

Query: 2487 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLC 4115
              NF HFG VVR+DM+FLK+G   GV WAN+ FRIPQ  K VDD+VWLRNLEDP  +     P WP+P YP LSG+DL+M DLKALEAY SYFY+ SK WSKPLPE YD ++VA YFS RPHVVA R+LEVFSSFASA + IR +GL+K L   +   +D+  S+YNFG+VLKETML+LGPTFIKVGQSLSTRPDIIG E+SKALSELHDQIPPFPR +AMKI+EEEL +P+ SFFSYISEEPVAAASFGQVY   T DG +VAVKVQRPNLRHVVVRD+YILRLGLGLLQKIAKRKSD RLYADELG+G VGELDYTLEAANA +F + HS FSFM+VPK+F HL+RKR LTMEWM GE      +   S S  + ++Y +RQKVDAKRRLLDLVNKGVEA+L QLLETG++HADPHPGNLR T+SG IGFLDFGLLC+MEK+HQFAMLASIVHIVNGDW+SL+ AL +MD+VRPGTNIR VTM+LE ALGEVE KDGIPD+KFSRVLGKI SVA KYHFRMP YYTLVLRSLAS EG ++ 
Sbjct:   33 LGNFGHFGQVVRKDMEFLKRGFNNGVAWANDAFRIPQIAKKVDDLVWLRNLEDPQ-ATSFSTPSWPEPWYPGLSGVDLLMYDLKALEAYASYFYHLSKIWSKPLPETYDPQDVAHYFSARPHVVALRMLEVFSSFASAGVSIRTSGLRKFLPINAEGGMDDKTSEYNFGLVLKETMLNLGPTFIKVGQSLSTRPDIIGVEMSKALSELHDQIPPFPRTVAMKILEEELGAPLESFFSYISEEPVAAASFGQVYFARTTDGVNVAVKVQRPNLRHVVVRDIYILRLGLGLLQKIAKRKSDLRLYADELGRGFVGELDYTLEAANALKFREVHSSFSFMRVPKIFLHLSRKRVLTMEWMVGE----SPTDLISVSTGNSTEYSDRQKVDAKRRLLDLVNKGVEATLVQLLETGLIHADPHPGNLRCTSSGEIGFLDFGLLCQMEKRHQFAMLASIVHIVNGDWASLVNALIDMDMVRPGTNIRLVTMELEQALGEVEFKDGIPDVKFSRVLGKILSVAFKYHFRMPAYYTLVLRSLASFEGLAIA 570          

HSP 2 Score: 68.1662 bits (165), Expect = 3.592e-8
Identity = 32/37 (86.49%), Postives = 34/37 (91.89%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLA+AAD  FKTFEAAYP+VVRKLLTENSA TRKILH
Sbjct:  566 GLAIAADKKFKTFEAAYPYVVRKLLTENSAATRKILH 602          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: A0A0A0KWU3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G623450 PE=4 SV=1)

HSP 1 Score: 794.653 bits (2051), Expect = 0.000e+0
Identity = 395/543 (72.74%), Postives = 458/543 (84.35%), Query Frame = -3

Query: 2487 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLC 4115
             AN+ HF DVVR+D++F+K G+ +G+ WAN+ FRIPQ  K+VDDV+WLRN+EDP  +     P  PQP YP LSG+DL MADLKALEAY  Y+Y  SK W+KPLPE YD E VA+YF  RPH+V  RLLEVFSSFASAAIRIRM+ ++K   PG+S   D + S+ NFG+VLKET+L+LGPTFIKVGQSLSTRPDIIG EISKALSELHDQIPPFPR +AMKII+EEL SP+ SFFSYISE+PVAAASFGQVYRG TLDG  VAVKVQRPN+ HVVVRDVYILRLGLG LQKIAKRK+D RLYADELGKGL+GELDY LEA NA++F++ HS F F+ VPKVF+HL+RKR LTMEW++G+       + T SS K  S Y ERQKVDA+RRLLDLVNKGVEA+L QLL+TG+LHADPHPGNLRY  SG+IGFLDFGLLCRME+KHQ AMLASIVH+VNG+W+SL++AL EMDVVRPGTN+RRVT+DLE ALGEVE K GIPD+KFS+VLGKIWS+ALKYHFRMPPYYTL+LRSLAS EG +L 
Sbjct:   38 LANWGHFADVVRKDVEFIKGGLGKGIRWANDAFRIPQVSKSVDDVLWLRNIEDPQ-AVNLPTPSRPQPSYPELSGVDLFMADLKALEAYAVYYYSLSKIWTKPLPEVYDPESVAEYFGCRPHIVGLRLLEVFSSFASAAIRIRMSRVQKF--PGTSLHEDSDESKSNFGLVLKETLLNLGPTFIKVGQSLSTRPDIIGSEISKALSELHDQIPPFPRTIAMKIIQEELGSPVESFFSYISEDPVAAASFGQVYRGRTLDGISVAVKVQRPNMLHVVVRDVYILRLGLGFLQKIAKRKNDLRLYADELGKGLLGELDYNLEARNATEFMETHSRFPFIHVPKVFRHLSRKRVLTMEWISGD---SPTELLTISSGKPSSTYSERQKVDARRRLLDLVNKGVEATLVQLLDTGLLHADPHPGNLRYIPSGQIGFLDFGLLCRMEEKHQVAMLASIVHLVNGEWASLVEALAEMDVVRPGTNMRRVTLDLEYALGEVEFKAGIPDVKFSKVLGKIWSLALKYHFRMPPYYTLMLRSLASFEGLALA 574          

HSP 2 Score: 201.445 bits (511), Expect = 1.106e-49
Identity = 106/235 (45.11%), Postives = 158/235 (67.23%), Query Frame = -1

Query:  470 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAY-------------LLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAIS 1135
            V+LN+KKEF+W+R+ LF+R+GA R+ LQ + A   E +++    + +    + Y             L+ RLL SK+G VLRRLIMT +GASL++A VSKEAK FR QLC I+ADI++Q  +K LGQ   A+     +R+ G   + +   S++L+T+  + DY S L+DRR++L+ SK+L SA  +P LML+F+W SFV F+ ASA+A HR+++SLS AY+GP S  PK++A++
Sbjct:  608 VILNKKKEFQWQRVVLFLRIGARRKGLQQMLAPHNEAAIELSAVKANNQAAIEYSTVKANSDLELVNLITRLLVSKEGAVLRRLIMTVNGASLIQAMVSKEAKFFRQQLCTIVADIIHQWALKTLGQGSQATGLGTTVRM-GILSDKKGRSSSQLTTTGQI-DYISFLNDRRMRLLFSKVLKSASTKPILMLKFFWTSFVIFVTASAVACHRIVVSLSEAYLGPISLSPKQYAVT 840          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: B9HUD9_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s22780g PE=4 SV=2)

HSP 1 Score: 794.267 bits (2050), Expect = 0.000e+0
Identity = 406/538 (75.46%), Postives = 472/538 (87.73%), Query Frame = -3

Query: 2499 ANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 4112
             NFSHFGD V +D +F+KKG+ +G++WANE FRIPQ  K +DD++WLRNLED N SPP +P  WPQP YP L+G+DL++ADLKALE+Y SYFY  SK WSKPLPEAYD +EVADYF+ RPH+VAFRLLEVF++FA+A IRIR +G++K LR GS +D++ N+SQY+ GMVLKETML+LGPTFIKVGQSLSTRPDIIG EI+KALS LHDQIPPFPR LAMKI EEEL SP+ SFFSY+SEEPVAAASFGQVYRG TLDG  VA+KVQRPNL HVVVRD+YI+RLGLGLLQKIAKRKSD RLYADELGKGLVGELDY++EAANAS+FLDAHS FSF+  PK+F  L+RKR LTMEW+ GE  TDLLS++T       S Y ERQK++AKRRLLDLV+KGVEASL QLLETG+LH DPHPGNLRY +SG+IGFLDFGLLC+MEKKH+FAMLA+IVHIVNGDW+SL+ AL +MDVVRPGT+IRR+TM+LE++LGEVE KDGIPD+KFSRVLGKI SVA+K HFRMPPY+TLVLRSLASLEG
Sbjct:   39 GNFSHFGDTVGKDFEFIKKGINKGMDWANEAFRIPQVSKTLDDILWLRNLEDHN-SPPIEPQSWPQPSYPGLTGVDLLLADLKALESYASYFYCLSKIWSKPLPEAYDPQEVADYFNCRPHLVAFRLLEVFTAFATATIRIRASGMRKFLRSGSDEDVNGNISQYDLGMVLKETMLNLGPTFIKVGQSLSTRPDIIGTEITKALSGLHDQIPPFPRTLAMKIFEEELGSPVESFFSYVSEEPVAAASFGQVYRGSTLDGRTVALKVQRPNLHHVVVRDIYIIRLGLGLLQKIAKRKSDLRLYADELGKGLVGELDYSIEAANASKFLDAHSSFSFIYAPKIFPDLSRKRVLTMEWVVGERPTDLLSLST------SSAYSERQKLEAKRRLLDLVSKGVEASLVQLLETGLLHGDPHPGNLRYISSGQIGFLDFGLLCQMEKKHRFAMLAAIVHIVNGDWASLVHALIDMDVVRPGTSIRRITMELENSLGEVEFKDGIPDVKFSRVLGKILSVAIKNHFRMPPYFTLVLRSLASLEG 569          

HSP 2 Score: 257.299 bits (656), Expect = 9.344e-68
Identity = 129/223 (57.85%), Postives = 169/223 (75.78%), Query Frame = -1

Query:  467 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAISA 1135
            VVLN++KEFRWERLALF+RVG+TR++   V AS  E+SLD LP R  GVFD A+L+LRLLPS+DG+VLR+L+MTA+GASL+RA VSKEA   R QLC++IAD LY  M +  G+ + A++Y  ++RL     N EL  S+RL+    VYDYQS++ DRRLK+I S+IL+SARK+P LML+FYW +FV  + AS  A HR+L+SLS A + P+ F+P R AISA
Sbjct:  607 VVLNKQKEFRWERLALFLRVGSTRKAFNRVIASKNESSLDYLPKRASGVFDTAHLVLRLLPSRDGIVLRKLLMTANGASLIRAMVSKEAIFVRQQLCRVIADALYHWMTQTFGRGIMATRYGSQVRLTSEADNRELSTSSRLTVP--VYDYQSIIRDRRLKVIFSRILDSARKDPVLMLKFYWTTFVMVVTASVRACHRVLVSLSEATLAPSRFLP-RVAISA 826          

HSP 3 Score: 75.485 bits (184), Expect = 2.851e-10
Identity = 35/37 (94.59%), Postives = 37/37 (100.00%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLAVAADP+FKTFEAAYP+VVRKLLTENSAETRKILH
Sbjct:  569 GLAVAADPNFKTFEAAYPYVVRKLLTENSAETRKILH 605          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: A0A059BI92_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_G02820 PE=4 SV=1)

HSP 1 Score: 791.571 bits (2043), Expect = 0.000e+0
Identity = 399/539 (74.03%), Postives = 462/539 (85.71%), Query Frame = -3

Query: 2499 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 4115
            F +FSHF D V++D  FL+K + RG+EWANE  RIP   K +DDVVWLR LE+P+ +PP +   WP+PCYP LSG+DL+MADL+ALEAY++YFY  S+ WS+PLPE Y+ E+V  YFS RPHVVA RLLEVF SFASA ++IR +G+   LR  S KD D N+SQYNFG+ LKETML+LGPTFIKVGQSLSTRPDIIG EISKALSELHDQIPPFPR +AM+ I+EE  SP  S FS+ISE+PVAAASFGQVYRG TLDG DVA+KVQRPNLRHVVVRD+YILRL LGL+QKIAKRKSD RLYADELGKGLVGELDYTLEA+NA++F + HSHF+FM+VPKV +HL+RKR LTMEWM GES TDLL ++TS+++  G  Y E Q  DAKRRLLDLV+KGVE++L QLLETG+LHADPHPGNLRYT++G+IGFLDFGLLC+ME++HQ AMLASIVHIVNGDW+SL+QALTEMDVVRPGTN+ R   DLEDALGEVE  DGIPD+KFSRVLGKIWSVALKYHFRMPPYYTLVLRSLAS EG
Sbjct:   47 FGDFSHFADAVKKDASFLRKRIGRGIEWANEALRIPLVAKTLDDVVWLRVLENPD-APPVEDCPWPRPCYPELSGLDLVMADLRALEAYMAYFYCLSRVWSRPLPEVYNVEDVNYYFSCRPHVVALRLLEVFFSFASATVKIRASGIASYLRLNSDKDNDGNISQYNFGLALKETMLNLGPTFIKVGQSLSTRPDIIGSEISKALSELHDQIPPFPRTVAMQTIKEEFGSPAQSIFSHISEDPVAAASFGQVYRGKTLDGLDVAIKVQRPNLRHVVVRDIYILRLALGLVQKIAKRKSDLRLYADELGKGLVGELDYTLEASNAAEFSETHSHFTFMRVPKVLEHLSRKRVLTMEWMVGESPTDLLEVSTSNAMVGGCAYSEEQIFDAKRRLLDLVSKGVESTLVQLLETGLLHADPHPGNLRYTSTGQIGFLDFGLLCQMERRHQLAMLASIVHIVNGDWASLIQALTEMDVVRPGTNLWR---DLEDALGEVEFTDGIPDVKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASFEG 581          

HSP 2 Score: 261.922 bits (668), Expect = 2.823e-69
Identity = 132/222 (59.46%), Postives = 166/222 (74.77%), Query Frame = -1

Query:  470 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAIS 1135
            VV  +KKEFRWERL+LF++VG TR+S+Q   A + + SLD +P+R    FDVA L+LRLLPSK GVVLRRL+MTADGASL+RA VSKE   FR QLC IIA+ILY  M+ A+G     +QY+ +++LA G  N EL PS+  STS  +YDYQS+  DRRL++I SK L SA+ +P LMLRF W S V F+ ASALA HR +++LS AY+GP SF+PKRFAIS
Sbjct:  619 VVFTKKKEFRWERLSLFLKVGVTRKSMQGSRALNDDASLDIIPNRNTSAFDVANLVLRLLPSKQGVVLRRLLMTADGASLIRAMVSKEGGFFRQQLCGIIAEILYHWMLGAIGSSTRVAQYNSRVKLASGTSNYELGPSSGRSTS--IYDYQSIFRDRRLRVIFSKALRSAQSDPVLMLRFCWSSLVIFVTASALACHRAVVNLSEAYLGPLSFVPKRFAIS 838          

HSP 3 Score: 70.4774 bits (171), Expect = 8.271e-9
Identity = 32/37 (86.49%), Postives = 36/37 (97.30%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLAVAADP+FKTF+AAYP+VVRKLLTEN+A TRKILH
Sbjct:  581 GLAVAADPNFKTFQAAYPYVVRKLLTENTAATRKILH 617          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: B9T4T1_RICCO (Ubiquinone biosynthesis protein coq-8, putative OS=Ricinus communis GN=RCOM_0332910 PE=4 SV=1)

HSP 1 Score: 786.178 bits (2029), Expect = 0.000e+0
Identity = 389/539 (72.17%), Postives = 441/539 (81.82%), Query Frame = -3

Query: 2499 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 4115
              NFSHFG+ V +D +F+KKGV+                                                 L+GMDL MADLKALEAY SYFYY SK WSKPLPE YD ++VADYFS RPHVVA RLLEVFS+FASA IRIR +G++K L+P S +D++ N+SQYNFG+VLKETML+LGPTFIKVGQSLSTRPDIIG EISKALSELHDQIPPFPR +AMKI+EEEL SP+ SFFS ISEEPVAAASFGQVYR  TLDG +VA+KVQRPNLRHVVVRD+YILRLGLGL+QKIAKRK+D RLYADELGKGLVGELDY+LEAANAS+F D HS F FM VPK++ HLTRKR LTMEW+ GES TDLLSI+  +++ HG  Y ERQK +AKRRLLDLV+KGVEASL QLLETG+LHADPHPGNLRYT+SG++GFLDFGLLC+MEKKHQFAMLASIVHIVNGDW SL++AL EMD+VRPGTN+RRVTM+LE++LGEVE +DGIPD+KFSRVL KIWSVALKYHFRMPPYYTLVLRSLASLEG
Sbjct:   43 IGNFSHFGETVHKDFEFIKKGVR-------------------------------------------------LTGMDLFMADLKALEAYASYFYYLSKLWSKPLPEVYDPQDVADYFSCRPHVVALRLLEVFSAFASATIRIRASGMRKFLQPNSDRDVNGNISQYNFGVVLKETMLNLGPTFIKVGQSLSTRPDIIGTEISKALSELHDQIPPFPRTMAMKIVEEELGSPVESFFSCISEEPVAAASFGQVYRANTLDGCNVALKVQRPNLRHVVVRDIYILRLGLGLVQKIAKRKNDLRLYADELGKGLVGELDYSLEAANASKFQDIHSSFKFMHVPKIYHHLTRKRVLTMEWVVGESPTDLLSISAGNAVDHGYAYSERQKTEAKRRLLDLVSKGVEASLVQLLETGLLHADPHPGNLRYTSSGQLGFLDFGLLCQMEKKHQFAMLASIVHIVNGDWESLVRALIEMDIVRPGTNLRRVTMELENSLGEVEFRDGIPDVKFSRVLSKIWSVALKYHFRMPPYYTLVLRSLASLEG 532          

HSP 2 Score: 243.817 bits (621), Expect = 1.545e-63
Identity = 131/224 (58.48%), Postives = 164/224 (73.21%), Query Frame = -1

Query:  467 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDG-VFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAISA 1135
            VVLN++KEFRW+RLALF+RVG+TR+ L    A   E+S D L +R+ G VFDVA+L+L LLPS+DG+ LR+L+MTADGASLVRA VSKEA  FR QL ++IAD+LYQ +VK LG    A++YS ++RL     N EL PS+ LS S  +YDYQS+  DRRLK+I S+ILNSA K P LML+  W S V  +AASALA HR+L+SLS  YI P S   K  A+SA
Sbjct:  570 VVLNKRKEFRWDRLALFLRVGSTRKVLNRAIAPKSESSFDYLTNRSSGGVFDVAHLVLLLLPSRDGIALRKLLMTADGASLVRAVVSKEAVFFRQQLSRVIADLLYQWVVKTLGIGNKATKYSSQVRLTSELDNKELGPSSNLSMS--MYDYQSIFQDRRLKVIFSRILNSAMKNPVLMLKLCWTSVVMVVAASALACHRVLVSLSEIYIAPFSLARKEVALSA 791          

HSP 3 Score: 72.4034 bits (176), Expect = 2.430e-9
Identity = 33/37 (89.19%), Postives = 36/37 (97.30%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLAVAADP+FKTFEAAYP+VVRKLLTENS ETR+ILH
Sbjct:  532 GLAVAADPNFKTFEAAYPYVVRKLLTENSNETRRILH 568          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: A5AE62_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_032583 PE=4 SV=1)

HSP 1 Score: 783.482 bits (2022), Expect = 0.000e+0
Identity = 400/540 (74.07%), Postives = 451/540 (83.52%), Query Frame = -3

Query: 2487 SHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFI-KVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLC 4103
            + FG+VV +DM+FLKK + RGV+WAN   RIPQ  K++D ++WLR  EDP  +    PP WPQP YP LSG+DL MADLKALE Y SYFY+ SK WSKPLPE YD  EVADYF+ RPH+VA RLLEVFSSFA AAIRIR +G+         +DI+ N+S YNFGM       S  P     VGQS+STRPDIIGPEISKALS LHDQIPPFPR +AMKIIEEEL SP+ +FF YISEEPVAAASFGQVYRG TLDGS+VAVKVQRPNL HVVVRD+YILR+GLGL+QKIAKRKSDPRLYADELGKGL GELDYTLEAANAS+FL+ HS FSF++VPKV +HL+RKR LTMEWM GE+ +DL+S +  +SI H S Y ERQ+ DAKRRLLDLVNKGVEASL QLL+TG+LHADPHPGNLRY  SG+IGFLDFGLLCRMEKKHQFAMLASIVHIVNGDW SL+ ALTEMDV+R GTNI+RVTMDLEDALGEVE KDGIPD+KFS+VLGKIWS+ALKYHFRMPPYYTLVLRSLASLEG ++ 
Sbjct:   33 ASFGEVVXKDMEFLKKRIGRGVQWANGALRIPQLSKSLDRLLWLRMTEDP-LAASLPPPSWPQPSYPGLSGVDLFMADLKALETYASYFYHLSKLWSKPLPEVYDPLEVADYFNRRPHIVALRLLEVFSSFAFAAIRIRTSGITMFYGSNMDRDINGNISPYNFGMSQDYKYASYMPLLADAVGQSISTRPDIIGPEISKALSGLHDQIPPFPRDVAMKIIEEELGSPVEAFFRYISEEPVAAASFGQVYRGITLDGSNVAVKVQRPNLHHVVVRDIYILRIGLGLVQKIAKRKSDPRLYADELGKGLSGELDYTLEAANASEFLETHSSFSFIRVPKVLRHLSRKRVLTMEWMVGENPSDLISASAGNSIAHVSGYSERQQTDAKRRLLDLVNKGVEASLVQLLDTGLLHADPHPGNLRYMPSGQIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWPSLVHALTEMDVIRAGTNIQRVTMDLEDALGEVEFKDGIPDVKFSKVLGKIWSIALKYHFRMPPYYTLVLRSLASLEGLAIA 571          

HSP 2 Score: 250.751 bits (639), Expect = 1.373e-65
Identity = 129/223 (57.85%), Postives = 166/223 (74.44%), Query Frame = -1

Query:  467 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAISA 1135
            VVLNR+KEF+W++L+LF+RVGATR+ LQ + A +GE  L+  P   +G  DVA L+LRLLPSKDGVVLRRL+MTADGASL+R  +SKEA  FR QLCK IAD+LYQRM++ +GQ +  +Q+S + RL  G  N +L  S+   +S+  YDYQS+L DRRLK+I  KI +S R++P L LRF W SF+ F+ ASALA HR+L+SLS  Y+GP S   KR AISA
Sbjct:  605 VVLNRRKEFQWQKLSLFLRVGATRKGLQQLVAPNGEAPLNYSPGGVNGTVDVANLVLRLLPSKDGVVLRRLLMTADGASLIRMMISKEAIFFRQQLCKAIADVLYQRMLEVIGQGIAITQHSSQWRLRSGPNNRDL--SSLSRSSALTYDYQSVLRDRRLKVIFFKIFDSVRRDPVLTLRFCWASFIMFMTASALACHRILVSLSEIYLGPVSLPSKRVAISA 825          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: A0A068U9S9_COFCA (Coffea canephora DH200=94 genomic scaffold, scaffold_12 OS=Coffea canephora GN=GSCOC_T00017736001 PE=4 SV=1)

HSP 1 Score: 775.007 bits (2000), Expect = 0.000e+0
Identity = 387/530 (73.02%), Postives = 461/530 (86.98%), Query Frame = -3

Query: 2499 RRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKD--IDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 4082
            ++D +FL+KG  +GVEWAN T  +P+  K++DD +WLR++EDP    P + P WPQP YP LSG+DL++ADL+ALEAY+ YFYY SK W+KPLPE+YD++EVADYF  RPHVVA RLLEVF++F+SAAIR+R++G+      GSSK    D+++SQY+FG++LKETML+LGPTFIK+GQSLSTRPDIIG EISKALSELH+ IP FP+ +A KIIEEEL SP+G FFSYISEEP+AAASFGQVYR  T+DG DVAVKVQRPNL HVVVRD+YILRLGLGLL++IAKRKSDPRLYADELGKGLVGELDYTLEAANA +F++AHS + F+ VPK+F+HL+RK+ LTMEWM G++  DLL ++T S  +H     ER++ +AKRRLLDLVNKGVEASL QLLETG+LHADPHPGNLRYT+SG+IGFLDFGLLC+MEKKHQFAMLASIVHIV GDW+SL+QAL +MDVVRPGTN+ RVTMDLEDALGEVEL  GIP++KFSRVLGKIWSVALKYHFRMPPYYTL+LRSLASLEG
Sbjct:   44 KKDFEFLRKGFSKGVEWANTTLHLPKIAKSIDDFIWLRHVEDPRVVSPLRTPSWPQPYYPELSGIDLLVADLQALEAYIRYFYYLSKLWTKPLPESYDSQEVADYFRLRPHVVALRLLEVFAAFSSAAIRMRISGI------GSSKSNVADKDISQYSFGILLKETMLNLGPTFIKIGQSLSTRPDIIGTEISKALSELHENIPSFPKVVAFKIIEEELGSPVGKFFSYISEEPIAAASFGQVYRARTVDGFDVAVKVQRPNLHHVVVRDIYILRLGLGLLKEIAKRKSDPRLYADELGKGLVGELDYTLEAANAEKFMEAHSRYPFICVPKIFRHLSRKKILTMEWMVGDNPRDLLFLSTESLDQHPG-LTERRQNEAKRRLLDLVNKGVEASLIQLLETGLLHADPHPGNLRYTSSGQIGFLDFGLLCQMEKKHQFAMLASIVHIVYGDWASLVQALIQMDVVRPGTNVLRVTMDLEDALGEVELNRGIPNVKFSRVLGKIWSVALKYHFRMPPYYTLLLRSLASLEG 566          

HSP 2 Score: 238.039 bits (606), Expect = 1.750e-61
Identity = 123/222 (55.41%), Postives = 158/222 (71.17%), Query Frame = -1

Query:  470 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAIS 1135
            VV NRKKEF+W++LALF+RVGA R+ L LV AS  ETS D       G  DVA L+LRLLPSKDG VLRRL+MTA+G SLVRA VSKEA + R Q C++IADILYQ + + LG  V   Q+S +++L  G  N E+  S+R+   + + DYQSLL DRR+K+IL KIL+SAR++P LMLRFYW SF+  + ASA+A HR+L++L  A +   S  PKR A+ 
Sbjct:  604 VVFNRKKEFQWKKLALFLRVGAARKGLHLVAASKSETSRDPSSIGVSGELDVANLILRLLPSKDGYVLRRLLMTANGTSLVRAMVSKEANSMRQQFCRVIADILYQWISRVLGNGVQVVQFSPQVQLGRGASNIEIDSSSRI---TPMIDYQSLLRDRRIKIILFKILDSARRDPILMLRFYWTSFIMLVTASAMACHRVLVTLFEASLAHISLAPKRIAVG 822          

HSP 3 Score: 66.6254 bits (161), Expect = 1.341e-7
Identity = 30/37 (81.08%), Postives = 35/37 (94.59%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLAVAADP+FKTFEAAYP+VVRKLLT+NSA + +ILH
Sbjct:  566 GLAVAADPNFKTFEAAYPYVVRKLLTDNSASSGRILH 602          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: W9RIR0_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_013421 PE=4 SV=1)

HSP 1 Score: 773.081 bits (1995), Expect = 0.000e+0
Identity = 399/533 (74.86%), Postives = 451/533 (84.62%), Query Frame = -3

Query: 2487 DMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAG--LKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFI-KVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLC 4076
            DM+F+K G+ +G+EWAN+ FRIPQ  KA+D+ VWLRN+EDPN + P   P WPQP YP LSG+DL MADLKALEAY +YFYY SK WSKPLPE YDA+ VADYF+ RPHVVAFRLLEVFSSFA+A IRIR +   L+K LR     DI+  LS+YNFG+       S        VGQSLSTRPDIIG EISKALSELHDQIPPFPR  AMKIIEEEL SP+ S FSYIS+EPVAAASFGQVY   TLDGS VAVKVQRPN+RHVVVRD+YILRLGLG+LQKIAKRKSD RLYADELGKGLVGELDYTLEAANAS+F++ HS FSFM+VPKV QHL++KR LTMEWM GES TDLLS++T SS+ + S Y ERQK DAKRRLLDLV+KGVEA+L QLLETG+LHADPHPGNLRYT+SG+IGFLDFGLLCRMEKKHQFAMLASIVHIVNGDW+SL+ ALT+MD++RPGTNIRRV +DLE ALGEVE +DGIPD+KFSRVLGKI S+ALKY FRMPPY+TL+LRSLAS EG +L 
Sbjct:   44 DMEFVKNGIGKGLEWANKAFRIPQVSKAIDEFVWLRNMEDPN-ASPQPSPSWPQPSYPGLSGVDLFMADLKALEAYGAYFYYLSKTWSKPLPEVYDAQRVADYFNCRPHVVAFRLLEVFSSFAAATIRIRTSDSRLRKFLRSSGDNDINGGLSEYNFGVSQDYQYASYMRLLADAVGQSLSTRPDIIGSEISKALSELHDQIPPFPRNEAMKIIEEELGSPVESVFSYISDEPVAAASFGQVYFASTLDGSTVAVKVQRPNMRHVVVRDIYILRLGLGILQKIAKRKSDLRLYADELGKGLVGELDYTLEAANASEFMEVHSSFSFMRVPKVLQHLSQKRVLTMEWMVGESPTDLLSMSTWSSVDNDSAYAERQKFDAKRRLLDLVSKGVEATLVQLLETGLLHADPHPGNLRYTSSGQIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWASLVYALTDMDIIRPGTNIRRVILDLEYALGEVEFRDGIPDLKFSRVLGKILSIALKYQFRMPPYFTLLLRSLASFEGLALA 575          

HSP 2 Score: 249.21 bits (635), Expect = 5.025e-65
Identity = 125/222 (56.31%), Postives = 166/222 (74.77%), Query Frame = -1

Query:  470 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAIS 1135
            VVLN+KKEF+W+RLALF+R GATR+ L  +  S  E ++ NLPS ++ +FD+A L+LRLL S DG VLRRL+MTADGAS+++A VSKEAK FR Q C++IAD+LY  + KALGQ +  ++Y  K+ L+ G  N EL  S+   +S  +YDY S+L DRRLK+I S IL SAR++P LMLRFYWVSFV  + ASA+A HR+++SLS AY GP S  PK++A+ 
Sbjct:  609 VVLNKKKEFQWQRLALFLRAGATRKGLNRMIVSRNEAAIKNLPSTSNNIFDLANLVLRLLHSNDGAVLRRLLMTADGASIIQAVVSKEAKVFREQFCRVIADVLYLWIFKALGQDITTTRYGSKVILSIGAGNREL--SSSSRSSMPIYDYDSILRDRRLKVIFSHILKSARRKPVLMLRFYWVSFVMLLTASAIACHRVVVSLSEAYFGPVSLAPKQYAMG 828