g.barbadense_cottongen_reftransV1_0097180, g.barbadense_cottongen_reftransV1_0097180 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy Swiss-Prot
Match: RPK2_ARATH (LRR receptor-like serine/threonine-protein kinase RPK2 OS=Arabidopsis thaliana GN=RPK2 PE=2 SV=1)

HSP 1 Score: 818.15 bits (2112), Expect = 0.000e+0
Identity = 540/1112 (48.56%), Postives = 683/1112 (61.42%), Query Frame = 2

Query:  449 KDPCLWHGVSCDPLEGRVITXXXXXXXXXTCSVLQLSASKTAEVKGDQVR------GNFTLLYPCLHVDVDDNISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDL------------------------GFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXG---PLFSRKGSSFMFHQEEYGE--GDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIPVPCMVVFNISQNSLYGNISRFSHGECSNGSLNLSM------SYMDLVGLYSSFFYRNA-----LINVGSGHSPFSLSEFVVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGE-MKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISLNCESVKENPNLQPCHT---D*SSSELERHHFGNISQG-GRSPRENIQKI---RSEFNQIEIASITSASVIFSVLIALIFFLVCMKKFACNAVSDHVSGRKEVVTCNSISIQLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLGRFQGVQQFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQERSRRTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWASMLLAQGRGCEFFMARLWDSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRLKAIQPPT 3622
            +D C W GVSCD                S+  V+ L+ S +   +  + R      G F L    +  D   N     L G L   I  LT LRVLSL FN F GE+P+ +  +  LEVLDL                        GFN   G IP +L+  T L ++NL GN+LNGT+P   G    F+V+    N L G +P ++G++CG L HL L+ N L+G IP +LG C  L+SL+L  N L+  IP   G L+ LE LD+SRN LSG +P  LG+C  L +LVL N       + S +G + +    +      D+NF++G +PE I +L  + +LW+P   LEG FP  WGSC NL+ +N+ QNF  G+IP     CKNL  LDLSSN L+G L   I VPCM VF++  NSL G I  F +   S+    +        SY D   +Y SFF   A     LI++GS   P       V H+F+ N FTG++    ++   L  +++Y F   GN   G      FD C  L  +  + S NK+ G +P  + + C  LKIL  + N+  G IPTS  D+ SL+ LNLS N+L G IP  +G+ M  + YLS++NNN +G +P    QL SL+VL+LSSN LSG I  D   LK+L+ L L++N L+G IP GF+     +VFNVS NNL+G +P  +    C +V  NP L+PCH       SS+       +I+Q    SP EN       +  FN +EIASI SAS I SVLIAL+      +K+   +     + ++EV     I + +T++NVVRATG FN  N IG+GGFGATYKAE+   VVVA+KRLS+GRFQGVQQF AEIKTLGR++HPNLVTLIGYH SE EMFL+YNYLPGGNLE FIQERS R  +WR+LHKIALDIA AL +LHD+CVPRVLHRD+KPSNILLD   NAYLSDFGLARLLGTSETHATT VAGTFGYVAPEYAMTCRVSDKADVYSYGVV+L LLSDKKALDPSF S+GNGFNIV WA MLL QGR  EFF A LWD+GP  DL+EVLHLAV+CT +S S+RP+M++V RRLK +QPP+
Sbjct:   72 EDYCSWFGVSCD----------------SSSRVMALNISGSGSSEISRNRFTCGDIGKFPLYGFGVRRDCTGNHG--ALAGNLPSVIMSLTGLRVLSLPFNSFSGEIPVGIWGMEKLEVLDLEGNLMTGSLPDQFTGLRNLRVMNLGFNRVSGEIPNSLQNLTKLEILNLGGNKLNGTVPGFVG---RFRVLHLPLNWLQGSLPKDIGDSCGKLEHLDLSGNFLTGRIPESLGKCAGLRSLLLYMNTLEETIPLEFGSLQKLEVLDVSRNTLSGPLPVELGNCSSLSVLVLSNLYNVYEDINSVRGEADLPPGADLTSMTEDFNFYQGGIPEEITRLPKLKILWVPRATLEGRFPGDWGSCQNLEMVNLGQNFFKGEIPVGLSKCKNLRLLDLSSNRLTGELLKEISVPCMSVFDVGGNSLSGVIPDFLNNTTSHCPPVVYFDRFSIESYSDPSSVYLSFFTEKAQVGTSLIDLGSDGGP------AVFHNFADNNFTGTLKSIPLAQERLGKRVSYIFSAGGNRLYGQFPGNLFDNCDELKAVYVNVSFNKLSGRIPQGLNNMCTSLKILDASVNQIFGPIPTSLGDLASLVALNLSWNQLQGQIPGSLGKKMAALTYLSIANNNLTGQIPQSFGQLHSLDVLDLSSNHLSGGIPHDFVNLKNLTVLLLNNNNLSGPIPSGFA---TFAVFNVSSNNLSGPVPSTNGLTKCSTVSGNPYLRPCHVFSLTTPSSDSRDSTGDSITQDYASSPVENAPSQSPGKGGFNSLEIASIASASAIVSVLIALVILFFYTRKWHPKS-KIMATTKREVTMFMDIGVPITFDNVVRATGNFNASNLIGNGGFGATYKAEISQDVVVAIKRLSIGRFQGVQQFHAEIKTLGRLRHPNLVTLIGYHASETEMFLVYNYLPGGNLEKFIQERSTR--DWRVLHKIALDIARALAYLHDQCVPRVLHRDVKPSNILLDDDCNAYLSDFGLARLLGTSETHATTGVAGTFGYVAPEYAMTCRVSDKADVYSYGVVLLELLSDKKALDPSFVSYGNGFNIVQWACMLLRQGRAKEFFTAGLWDAGPHDDLVEVLHLAVVCTVDSLSTRPTMKQVVRRLKQLQPPS 1150          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy Swiss-Prot
Match: RPK1_ARATH (Probable LRR receptor-like serine/threonine-protein kinase RPK1 OS=Arabidopsis thaliana GN=RPK1 PE=1 SV=1)

HSP 1 Score: 440.269 bits (1131), Expect = 1.588e-138
Identity = 246/451 (54.55%), Postives = 309/451 (68.51%), Query Frame = 2

Query: 2264 GEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISLNCESVKENPNLQPCHTD*SSSELERHHFGNISQGGRSPRENIQKIRSEFNQIEIASITSASVIFSVLIALIFFLVCMKKFACNAVSDHVSGRKEVVTCNSISIQLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLGRFQGVQQFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQERSRRTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWASMLLAQGRGCEFFMARLWDSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRLKAIQP 3616
            G I P + +L  +  L L  N L G+IP     +  L + ++  NN  G I           V +N  L+   +           F +  + G S  ++    +S    IEIASI SASVI  VL+ L+   +  +K+  N+    V   KE+     I I LTYE +VRATG F+  NCIG GGFG+TYKAEV P  V AVKRLS+GRFQG QQF AEI  L  V+HPNLV LIGYH SE EMFLIYNYL GGNL++FI+ERS+  +EW++LHKIALD+A AL +LH++C P+VLHRDIKPSNILLD ++NAYLSDFGL++LLGTS++H TT VAGTFGYVAPEYAMTCRVS+KADVYSYG+V+L L+SDK+ALDPSFSS  NGFNIV+WA M+L+QG+  E F   LW++GP  DL+EVLHLA+ CT +S S RP+M++  R LK IQP
Sbjct:  109 GVISPVVGDLSEIRVLSLSFNDLRGEIPKEIWGLEKLEILDLKGNNFIGGI----------RVVDNVVLRKLMS-----------FEDEDEIGPSSADDDSPGKSGLYPIEIASIVSASVIVFVLLVLVILFIYTRKWKRNS-QVQVDEIKEIKVFVDIGIPLTYEIIVRATGYFSNSNCIGHGGFGSTYKAEVSPTNVFAVKRLSVGRFQGDQQFHAEISALEMVRHPNLVMLIGYHASETEMFLIYNYLSGGNLQDFIKERSKAAIEWKVLHKIALDVARALSYLHEQCSPKVLHRDIKPSNILLDNNYNAYLSDFGLSKLLGTSQSHVTTGVAGTFGYVAPEYAMTCRVSEKADVYSYGIVLLELISDKRALDPSFSSHENGFNIVSWAHMMLSQGKAKEVFTTGLWETGPPDDLVEVLHLALKCTVDSLSIRPTMKQAVRLLKRIQP 537          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy Swiss-Prot
Match: Y2317_ARATH (Probable leucine-rich repeat receptor-like protein kinase At2g33170 OS=Arabidopsis thaliana GN=At2g33170 PE=2 SV=1)

HSP 1 Score: 369.007 bits (946), Expect = 2.056e-106
Identity = 324/1038 (31.21%), Postives = 512/1038 (49.33%), Query Frame = 2

Query:  647 NISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXX--XXXGPLFSRKGSS------FMFHQEEYG-------------EGDY--NFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIPV--PCMVVFNISQNSLYGNISRFSHGECSNGSLNLSMSYM-----DLVGLYSSFFYRNALINVGSGHSPFSLSEFVVLH--DFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLI-FDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXX-XGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISLNCESVKENPNLQPCHTD*SSSELERHHFGNIS--QGGRSPRENIQKIRSEFNQIEIASITSASVIFSVLIALIFFLVCMKKFACNAVSDHVSGRKEVVTCNSISI----QLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLGRFQGVQQ-------FAAEIKTLGRVQHPNLVTLIG--YHVSEAEMFLIYNYLPGGNLENFIQERSRRTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWASMLLAQ----GRGCEFFMARLWDSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRL 3601
            ++S + L G +SP+IG L  L  L+L +N   G++P E+G  + LEV+ L  N F G IP  + K + LR  N+  N+L+G +P   G + + + +    N L+G +P  LG N   L       N+ SG+IP+ +G C  LK L L+ N +  ++P  +G L  L+ + L +N  SG +P  +G+   L+ L L   +  GP+ S  G+       +++  +  G             E D+  N   GE+P  + K+  + +L+L    L GI P       NL +L+++ N LTG IP  F N  ++  L L  N+LSG +P  + +  P  VV + S+N L G I  F   + +   LNL  + +       V    S      + N  +G  P  L + V L   +  +N+F+G +PP I +   L         L  N F  NL     +   +L  L+ F+ S+N + G +P  + + CK L+ L L+ N F+GS+P     +  L  L LSENR  G IP  IG +  +  L +  N FSG++P  L  LSSL++    S +  SGEI P++  L  L  L L++N L+G+IP  F N+++L   N S+NNLTG +P   I  N        N   C     S +     + +IS  + G + R                 I  +SVI  + + LI  +V   +      + +V  ++     + I      + T ++++ AT  F+    +G G  G  YKA +  G  +AVK+L   R             F AEI TLG+++H N+V L    YH       L+Y Y+  G+L   +      +++W     IAL  A  L +LH +C PR++HRDIK +NIL+D++F A++ DFGLA+++    + + + VAG++GY+APEYA T +V++K D+YS+GVV+L LL+ K  + P       G ++ TW    +          + ++ ++ D    + +I V  +AV+CT  SPS RP+MREV   L
Sbjct:   91 DLSSMNLSGIVSPSIGGLVNLVYLNLAYNALTGDIPREIGNCSKLEVMFLNNNQFGGSIPVEINKLSQLRSFNICNNKLSGPLPEEIGDLYNLEELVAYTNNLTGPLPRSLG-NLNKLTTFRAGQNDFSGNIPTEIGKCLNLKLLGLAQNFISGELPKEIGMLVKLQEVILWQNKFSGFIPKDIGNLTSLETLALYGNSLVGPIPSEIGNMKSLKKLYLYQNQLNGTIPKELGKLSKVMEIDFSENLLSGEIPVELSKISELRLLYLFQNKLTGIIPNELSKLRNLAKLDLSINSLTGPIPPGFQNLTSMRQLQLFHNSLSGVIPQGLGLYSPLWVV-DFSENQLSGKIPPFICQQSNLILLNLGSNRIFGNIPPGVLRCKSLLQLRVVGNRLTGQFPTELCKLVNLSAIELDQNRFSGPLPPEIGTCQKLQR-----LHLAANQFSSNLP----NEISKLSNLVTFNVSSNSLTGPIPSEIAN-CKMLQRLDLSRNSFIGSLPPELGSLHQLEILRLSENRFSGNIPFTIGNLTHLTELQMGGNLFSGSIPPQLGLLSSLQIAMNLSYNDFSGEIPPEIGNLHLLMYLSLNNNHLSGEIPTTFENLSSLLGCNFSYNNLTGQLPHTQIFQNMTLTSFLGNKGLCGGHLRSCDPSHSSWPHISSLKAGSARRGR-------------IIIIVSSVIGGISLLLIAIVVHFLRNPVEPTAPYVHDKEPFFQESDIYFVPKERFTVKDILEATKGFHDSYIVGRGACGTVYKAVMPSGKTIAVKKLESNREGNNNNSNNTDNSFRAEILTLGKIRHRNIVRLYSFCYHQGSNSNLLLYEYMSRGSLGELLHGGKSHSMDWPTRFAIALGAAEGLAYLHHDCKPRIIHRDIKSNNILIDENFEAHVGDFGLAKVIDMPLSKSVSAVAGSYGYIAPEYAYTMKVTEKCDIYSFGVVLLELLTGKAPVQP----LEQGGDLATWTRNHIRDHSLTSEILDPYLTKVEDDVILNHMITVTKIAVLCTKSSPSDRPTMREVVLML 1099          

HSP 2 Score: 111.694 bits (278), Expect = 2.270e-23
Identity = 130/452 (28.76%), Postives = 189/452 (41.81%), Query Frame = 2

Query: 1070 LKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIPVPCMVVFNISQNSLYGNISRFSHGECSNGSLNLSMSYMDLVGLYSSFFYRNALINVGSGHSPFSLSEFVVLHD--FSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIP 2419
            + SL LSS  L   +  S+G L NL  L+L+ N L+G +P  +G+C                S+    F+         + N F G +P  I KL  +    + N  L G  P+  G   NL+ L    N LTG +P S GN   L                         F   QN   GNI     G+C N         + L+GL  +F          SG  P  +   V L +    +N+F+G +P  I +L +L     YG                                N +VG +P  +G+  K LK L L  N+  G+IP     +  +++++ SEN L G IP  + ++ E+R L L  N  +G +P +L +L +L  L+LS NSL+G I P    L  +  L+L HN L+G IP G    + L V + S N L+G IP
Sbjct:   87 VTSLDLSSMNLSGIVSPSIGGLVNLVYLNLAYNALTGDIPREIGNC----------------SKLEVMFL---------NNNQFGGSIPVEINKLSQLRSFNICNNKLSGPLPEEIGDLYNLEELVAYTNNLTGPLPRSLGNLNKL-----------------------TTFRAGQNDFSGNIPT-EIGKCLN---------LKLLGLAQNFI---------SGELPKEIGMLVKLQEVILWQNKFSGFIPKDIGNLTSLETLALYG--------------------------------NSLVGPIPSEIGNM-KSLKKLYLYQNQLNGTIPKELGKLSKVMEIDFSENLLSGEIPVELSKISELRLLYLFQNKLTGIIPNELSKLRNLAKLDLSINSLTGPIPPGFQNLTSMRQLQLFHNSLSGVIPQGLGLYSPLWVVDFSENQLSGKIP 438          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy Swiss-Prot
Match: Y5639_ARATH (Probable leucine-rich repeat receptor-like protein kinase At5g63930 OS=Arabidopsis thaliana GN=At5g63930 PE=2 SV=1)

HSP 1 Score: 363.999 bits (933), Expect = 1.046e-104
Identity = 332/1044 (31.80%), Postives = 519/1044 (49.71%), Query Frame = 2

Query:  647 NISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSF-QVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXX--XXXXXGPLFSRKGS----SFMFH---------QEEYGEGDY--------NFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIP-VPCMVVFNISQNSLYGNISRFS--HGE-------CSNGSLNLSMSYMDLVGLYSSFFYRNALINVGSGHSPFSLSEFVVLH--DFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLI-----FDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXX-XXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPL-NSISLNCESVKEN---PNLQPCHTD*SSSELERHHFGNISQGGRSPRENIQKIRSEFNQIEIASITSASV--IFSVLIALIFFLVCMKKFACNAVSDHVSGRKEVVTCNSI---SIQLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLGRFQG-----VQQFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQERSRRTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWA-SMLLAQGRGCEFFMARLW--DSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRL 3601
            N+S + L G+LSP+IG L  L+ L L +N   G++P E+G  + LE+L L  N F G IP  + K  SL  + +  N+++G++P   G + S  Q+V +S N +SG++P  +G N   L       N +SGS+PS +G C  L  L L+ N L  ++P  +G L+ L  + L  N  SG +P  + +C  L+ L        GP+    G      F++            E G   Y        N   GE+P  +  + G+ +L+L    L G  P    +  NL +L+++ N LTG IP  F   + L+ L L  N+LSG++P  +     + V ++S N L G I  +   H          +N S N+         L      RN L+    G  P +L + V +   +  +N+F GS+P  + +   L         L  N F G L         R  G++      + S+NK+ GE+P  +   CK L+ L +  N F G++P+    +  L  L LS N L G IP  +G +  +  L +  N F+G++P +L  L+     L LS N L+GEI P+L+ L  L  L L++N L+G+IP  F+N+++L  +N S+N+LTG IPL  +IS++     E    P L  C        ++   F      G+        +RS     +I +IT+A +  +  +LIALI +L  M++      S    G+   ++ +         T++++V AT  F+    +G G  G  YKA +  G  +AVK+L+     G        F AEI TLG ++H N+V L G+   +    L+Y Y+P G+L   + + S   ++W    KIAL  A  L +LH +C PR+ HRDIK +NILLD  F A++ DFGLA+++    + + + +AG++GY+APEYA T +V++K+D+YSYGVV+L LL+ K  + P       G ++V W  S +           ARL   D      ++ VL +A++CT  SP +RPSMR+V   L
Sbjct:   79 NLSSMVLSGKLSPSIGGLVHLKQLDLSYNGLSGKIPKEIGNCSSLEILKLNNNQFDGEIPVEIGKLVSLENLIIYNNRISGSLPVEIGNLLSLSQLVTYS-NNISGQLPRSIG-NLKRLTSFRAGQNMISGSLPSEIGGCESLVMLGLAQNQLSGELPKEIGMLKKLSQVILWENEFSGFIPREISNCTSLETLALYKNQLVGPIPKELGDLQSLEFLYLYRNGLNGTIPREIGNLSYAIEIDFSENALTGEIPLELGNIEGLELLYLFENQLTGTIPVELSTLKNLSKLDLSINALTGPIPLGFQYLRGLFMLQLFQNSLSGTIPPKLGWYSDLWVLDMSDNHLSGRIPSYLCLHSNMIILNLGTNNLSGNIPTGITTCKTLVQLRLARNNLV----GRFPSNLCKQVNVTAIELGQNRFRGSIPREVGNCSALQR-----LQLADNGFTGELP--------REIGMLSQLGTLNISSNKLTGEVPSEI-FNCKMLQRLDMCCNNFSGTLPSEVGSLYQLELLKLSNNNLSGTIPVALGNLSRLTELQMGGNLFNGSIPRELGSLTGLQIALNLSYNKLTGEIPPELSNLVMLEFLLLNNNNLSGEIPSSFANLSSLLGYNFSYNSLTGPIPLLRNISMSSFIGNEGLCGPPLNQC--------IQTQPFAPSQSTGKP-----GGMRSS----KIIAITAAVIGGVSLMLIALIVYL--MRRPVRTVASSAQDGQPSEMSLDIYFPPKEGFTFQDLVAATDNFDESFVVGRGACGTVYKAVLPAGYTLAVKKLASNHEGGNNNNVDNSFRAEILTLGNIRHRNIVKLHGFCNHQGSNLLLYEYMPKGSLGEILHDPSC-NLDWSKRFKIALGAAQGLAYLHHDCKPRIFHRDIKSNNILLDDKFEAHVGDFGLAKVIDMPHSKSMSAIAGSYGYIAPEYAYTMKVTEKSDIYSYGVVLLELLTGKAPVQP----IDQGGDVVNWVRSYIRRDALSSGVLDARLTLEDERIVSHMLTVLKIALLCTSVSPVARPSMRQVVLML 1078          

HSP 2 Score: 124.79 bits (312), Expect = 1.918e-27
Identity = 121/410 (29.51%), Postives = 189/410 (46.10%), Query Frame = 2

Query:  623 CLHVDVDDNISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENC--GSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIP-VPCMVVFNISQNSLYGNISRFSHGECSNGSLNLSMSYMDLVGLYSSFFYRNALIN-------VGSGHSPFSLSEFVVL--HDFSRNQFTGSVP 1816
             + +D  +N     L G +   +G++  L +L L  N+  G +P+E+  L  L  LDL  NA  GPIP   +    L ++ L  N L+GTIP   G  +   V+D S N LSG IP+ L   C   +++ L+L +N LSG+IP+ +  C  L  L L+ N L    PS+L K  N+ A++L +N   G +P  +G+C                          + +  +   N F GELP  I  L  +  L + +  L G  P    +C  L+RL++  N  +G +P+  G+   L  L LS+NNLSG++P A+  +  +    +  N   G+I R   G  +   + L++SY  L G          ++          SG  P S +    L  ++FS N  TG +P
Sbjct:  315 AIEIDFSEN----ALTGEIPLELGNIEGLELLYLFENQLTGTIPVELSTLKNLSKLDLSINALTGPIPLGFQYLRGLFMLQLFQNSLSGTIPPKLGWYSDLWVLDMSDNHLSGRIPSYL---CLHSNMIILNLGTNNLSGNIPTGITTCKTLVQLRLARNNLVGRFPSNLCKQVNVTAIELGQNRFRGSIPREVGNCSA----------------------LQRLQLAD---NGFTGELPREIGMLSQLGTLNISSNKLTGEVPSEIFNCKMLQRLDMCCNNFSGTLPSEVGSLYQLELLKLSNNNLSGTIPVALGNLSRLTELQMGGNLFNGSIPR-ELGSLTGLQIALNLSYNKLTGEIPPELSNLVMLEFLLLNNNNLSGEIPSSFANLSSLLGYNFSYNSLTGPIP 691          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy Swiss-Prot
Match: Y1723_ARATH (Leucine-rich repeat receptor-like serine/threonine-protein kinase At1g17230 OS=Arabidopsis thaliana GN=At1g17230 PE=2 SV=2)

HSP 1 Score: 359.762 bits (922), Expect = 3.076e-103
Identity = 332/1053 (31.53%), Postives = 502/1053 (47.67%), Query Frame = 2

Query:  623 CLHVDVDDNISFVG--LRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGE-----------------------NCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIP-VPCMVVFNISQNSLYGNISRFSHGECSNGSLNLSMSYMDLVGLYSSFFYR-NALI------NVGSGHSPFSLSEFVVLHD--FSRNQFTGSVPPFIISLYTLSAKLNYGFWLNG-------------------NNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPD-LAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISLNCESVKENPNLQPCHTD*SSSELERHHFGNISQGGRSPRENIQKIRSEFNQIEIASITSASVIFSVLIALIFFLVCMKKFACNAVSDHVSGRKEVVTCNSISIQ-LTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLGRFQGVQ---QFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQERSRRT-VEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWASMLLAQG-RGCEFFMARLW--DSGPQHDLIEVLHLAVMCTGESPSSRPSMREVA 3592
            C H+    ++   G  L G LSP I  L  LR L++  N   G +P ++     LEVLDL  N FHG IP  L    +L+ + L  N L G+IP   G ++S Q +    N L+G IP  + +                        C SL  L LA N L GS+P  L     L  LIL  N L  +IP S+G +  LE L L  N+ +G +P  +G                  ++    +++          N   GE+P  I  L     +      L G  P+ +G   NLK L++ +N L G IP   G    L  LDLS N L+G++P  +  +P +V   +  N L G I     G  SN S+ L MS   L G   + F R   LI      N  SG+ P  L     L       NQ TGS+P  + +L  L+A   +  WL+G                   NNF G +         ++ G  F+ S+N++ G +P  +G +C  ++ L L+ N+F G I      +V L  L LS+NRL G IP   G++  +  L L  N  S  +P +L +L+SL++    S++     +PD L  L+ L  L L+ NKL+G+IP    N+ +L + N+S NNL G++P  ++    +S     N   C++       +R H   +     S    +         + I  I   SV     + L  + +  ++ A  A+ D    + +V+       +  TY+ +V AT  F+    +G G  G  YKAE+  G V+AVK+L+  R +G      F AEI TLG+++H N+V L G+   +    L+Y Y+  G+L   +Q   +   ++W   ++IAL  A  L +LH +C P+++HRDIK +NILLD+ F A++ DFGLA+L+  S + + + VAG++GY+APEYA T +V++K D+YS+GVV+L L++ K  + P       G ++V W    +       E F ARL   D    H++  VL +A+ CT  SP+SRP+MREV 
Sbjct:   63 CTHLRTVTSVDLNGMNLSGTLSPLICKLHGLRKLNVSTNFISGPIPQDLSLCRSLEVLDLCTNRFHGVIPIQLTMIITLKKLYLCENYLFGSIPRQIGNLSSLQELVIYSNNLTGVIPPSMAKLRQLRIIRAGRNGFSGVIPSEISGCESLKVLGLAENLLEGSLPKQLEKLQNLTDLILWQNRLSGEIPPSVGNISRLEVLALHENYFTGSIPREIGK----------------LTKMKRLYLY---------TNQLTGEIPREIGNLIDAAEIDFSENQLTGFIPKEFGHILNLKLLHLFENILLGPIPRELGELTLLEKLDLSINRLNGTIPQELQFLPYLVDLQLFDNQLEGKIPPLI-GFYSNFSV-LDMSANSLSGPIPAHFCRFQTLILLSLGSNKLSGNIPRDLKTCKSLTKLMLGDNQLTGSLPIELFNLQNLTALELHQNWLSGNISADLGKLKNLERLRLANNNFTGEIPP-EIGNLTKIVG--FNISSNQLTGHIPKELG-SCVTIQRLDLSGNKFSGYIAQELGQLVYLEILRLSDNRLTGEIPHSFGDLTRLMELQLGGNLLSENIPVELGKLTSLQISLNISHNNLSGTIPDSLGNLQMLEILYLNDNKLSGEIPASIGNLMSLLICNISNNNLVGTVPDTAVFQRMDSSNFAGNHGLCNS-------QRSHCQPLVPHSDSKLNWLINGSQRQKILTITCIVIGSVFLITFLGLC-WTIKRREPAFVALEDQT--KPDVMDSYYFPKKGFTYQGLVDATRNFSEDVVLGRGACGTVYKAEMSGGEVIAVKKLN-SRGEGASSDNSFRAEISTLGKIRHRNIVKLYGFCYHQNSNLLLYEYMSKGSLGEQLQRGEKNCLLDWNARYRIALGAAEGLCYLHHDCRPQIVHRDIKSNNILLDERFQAHVGDFGLAKLIDLSYSKSMSAVAGSYGYIAPEYAYTMKVTEKCDIYSFGVVLLELITGKPPVQP----LEQGGDLVNWVRRSIRNMIPTIEMFDARLDTNDKRTVHEMSLVLKIALFCTSNSPASRPTMREVV 1069          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy Swiss-Prot
Match: PSKR1_DAUCA (Phytosulfokine receptor 1 OS=Daucus carota GN=PSKR PE=1 SV=1)

HSP 1 Score: 355.525 bits (911), Expect = 2.363e-102
Identity = 315/1036 (30.41%), Postives = 489/1036 (47.20%), Query Frame = 2

Query:  665 LRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPA-IFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIP----VPCMVVFNISQNSLYGNISRFSHGECSN-GSLNLSMSYM--DLVGLYSSFFYRNALINVGS----GHSP--FSLSEFVVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEF----------------VGSI----PTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIP--LNSISLNCESVKENPNL-----QPCH-TD*SSSELERHHFGNISQGGRSPRENIQKIRSEFNQIEIASITSASVIFSVLIALIFFLVCMKKFACN----AVSDHVS-GRKEVVTCNSISI--QLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLGRFQGVQQFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQER--SRRTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALD---PSFSSFGNGFNIVTWASMLLAQGRGCEFFMARLWDSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRLKAI 3610
            L G+LS ++  L +L+VL+L  N   G +   +  L+ LEVLDL  N F G  P+ L    SLRV+N+  N  +G IPA +   +   + +D + N   G IP  +G NC S+ +L LASN LSGSIP  L     L  L L +N L   + S LGKL NL  LD+S N  SG +P                               ++  Y     N F GE+P S+     I +L L N  L G       + +NL  L++A N  +G IP++  NC  L  ++ +       +P +      +  +   N S  ++   +    H  C N  +L L++++   +L  + S  F    ++ + S    G  P   S S  + L D S NQ +G++PP++ SL +L       F+L                         D SNN  +GE+P    H+   L+ L    N                   G +    P+SF  M+     +LS N L G I    G+++++  L+L NNN SG +P                         +L+ +  L  L L HN L+G IP     ++ LS F+V++N L+G IP  +   +    S + N  L      PCH TD      +  H   +       ++NI+KI      + +A  T    +F + + L+  L    +   +    A +D +  G + VV  ++     +L+ ++++++T  FN  N IG GGFG  YKA +  G  VA+KRLS    Q  ++F AE++TL R QHPNLV L+GY   + +  LIY+Y+  G+L+ ++ E+     +++W+   +IA   A  L +LH  C P +LHRDIK SNILL   F A+L+DFGLARL+   +TH TTD+ GT GY+ PEY      + K DVYS+GVV+L LL+ ++ +D   P  S      ++++W   +  + R  E F   ++D     +++ VL +A  C GE+P +RP+ +++   L+ I
Sbjct:   98 LSGKLSESVAKLDQLKVLNLTHNSLSGSIAASLLNLSNLEVLDLSSNDFSGLFPS-LINLPSLRVLNVYENSFHGLIPASLCNNLPRIREIDLAMNYFDGSIPVGIG-NCSSVEYLGLASNNLSGSIPQELFQLSNLSVLALQNNRLSGALSSKLGKLSNLGRLDISSNKFSGKIPDVF-------------------------LELNKLWYFSAQSNLFNGEMPRSLSNSRSISLLSLRNNTLSGQIYLNCSAMTNLTSLDLASNSFSGSIPSNLPNCLRLKTINFAKIKFIAQIPESFKNFQSLTSLSFSNSSIQNISSALEILQH--CQNLKTLVLTLNFQKEELPSVPSLQFKNLKVLIIASCQLRGTVPQWLSNSPSLQLLDLSWNQLSGTIPPWLGSLNSL-------FYL-------------------------DLSNNTFIGEIP----HSLTSLQSLVSKENAVEEPSPDFPFFKKKNTNAGGLQYNQPSSFPPMI-----DLSYNSLNGSIWPEFGDLRQLHVLNLKNNNLSGNIP------------------------ANLSGMTSLEVLDLSHNNLSGNIPPSLVKLSFLSTFSVAYNKLSGPIPTGVQFQTFPNSSFEGNQGLCGEHASPCHITD------QSPHGSAVKS-----KKNIRKI------VAVAVGTGLGTVFLLTVTLLIILRTTSRGEVDPEKKADADEIELGSRSVVLFHNKDSNNELSLDDILKSTSSFNQANIIGCGGFGLVYKATLPDGTKVAIKRLSGDTGQMDREFQAEVETLSRAQHPNLVHLLGYCNYKNDKLLIYSYMDNGSLDYWLHEKVDGPPSLDWKTRLRIARGAAEGLAYLHQSCEPHILHRDIKSSNILLSDTFVAHLADFGLARLILPYDTHVTTDLVGTLGYIPPEYGQASVATYKGDVYSFGVVLLELLTGRRPMDVCKPRGSR-----DLISWVLQMKTEKRESEIFDPFIYDKDHAEEMLLVLEIACRCLGENPKTRPTTQQLVSWLENI 1017          

HSP 2 Score: 73.9442 bits (180), Expect = 8.424e-12
Identity = 86/342 (25.15%), Postives = 133/342 (38.89%), Query Frame = 2

Query:  647 NISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPN-------------------------ELGENCGSLMHLHL-------------------------ASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXG----PLFSRKGSS---FMFHQEE----YGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASF 1489
            +IS     G++      L KL   S   N F GE+P  +     + +L L  N   G I       T+L  ++L+ N  +G+IP+        + ++F+  K   +IP                          E+ ++C +L  L L                         AS +L G++P  L N   L+ L LS N L   IP  LG L +L  LDLS N   G +P +L   + L       +      P F +K ++     ++Q        +  YN   G +      L  +HVL L N NL G  P      ++L+ L+++ N L+G IP S 
Sbjct:  260 DISSNKFSGKIPDVFLELNKLWYFSAQSNLFNGEMPRSLSNSRSISLLSLRNNTLSGQIYLNCSAMTNLTSLDLASNSFSGSIPSNLPNCLRLKTINFAKIKFIAQIPESFKNFQSLTSLSFSNSSIQNISSALEILQHCQNLKTLVLTLNFQKEELPSVPSLQFKNLKVLIIASCQLRGTVPQWLSNSPSLQLLDLSWNQLSGTIPPWLGSLNSLFYLDLSNNTFIGEIPHSLTSLQSLVSKENAVEEPSPDFPFFKKKNTNAGGLQYNQPSSFPPMIDLSYNSLNGSIWPEFGDLRQLHVLNLKNNNLSGNIPANLSGMTSLEVLDLSHNNLSGNIPPSL 601          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy Swiss-Prot
Match: MSP1_ORYSJ (Leucine-rich repeat receptor protein kinase MSP1 OS=Oryza sativa subsp. japonica GN=MSP1 PE=1 SV=1)

HSP 1 Score: 358.221 bits (918), Expect = 9.529e-102
Identity = 338/1135 (29.78%), Postives = 524/1135 (46.17%), Query Frame = 2

Query:  599 GNFTLLYPCLHVDVDDNISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXX--XXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLN-----------------------VAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAI-PVPCMVVFNISQNSLYGNISRFSHGECSNGSLNLSMSYMDLVGLYS------------SFFYRNALINVGSGHSPFSLSEFVVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELK------------------HLSALRLDHNKLTGQIPF----------------------------GFSNMTALSVFNVSFNNLTGSIP--LNSI-SLNCESVKENPNLQ---PC---------HTD*SSSELERHHFGNISQGGR-SPRENIQKIRSEFNQIEIASITSASVIFSVLIALIFFLVCMKK-------FACNAVS-----------DHVSGRKE-------VVTCNSISIQLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLG-RFQGVQQFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQERSR--RTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWASMLLAQGRGCEFF-----MARLWDSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRLK 3604
            GN + L   LH D   N     L G + P I  LT L  L L  N F G +P E+G+L  LE+L LG N   G IP  +     L++++L   Q  G IP     ++S   +D S N    E+P+ +GE  G+L  L   +  LSG++P  LGNC +L  + LS N L   IP     LE + +  +  N LSG VP  +   K  + +       +GPL        +    E      N   G +P  I +   +H L L + NL G   + +  C+NL  LN                       ++QN   G +PA     K L  + LS+N ++G +P +I  +  +   +I  N L G I + S G+  N + NLS+    L G+                 Y N   N+ S  S  +L + ++L   S NQ +GS+P  I   +   A  +  F                   L+  GL+ D S N++ G++P ++   C  + +L+L  N   G+IP    ++ +L  +NLS N   GP+  + G + +++ L LSNN+  G++P                 +  G+ILP +A L                   +L+ L + +N L+G I F                              SN T LS  ++  N+LTG +P  L+ + SLN   +  N NL    PC           + S + ++ +   + + GG  S      K    ++++  A IT  +  F ++I L+   V +++        A  + S           D + G+K        + T     +++T +++++AT  F+  + IG GGFG  YKA +  G  VA+KRL  G +FQG ++F AE++T+G+V+HPNLV L+GY V   E FLIY Y+  G+LE +++ R+     + W    KI L  A  L  LH   VP ++HRD+K SNILLD++F   +SDFGLAR++   ETH +TD+AGTFGY+ PEY +T + + K DVYS+GVV+L LL+ +            G N+V W   ++A+G+  E F     ++ +W    +  +  VL +A  CT + P  RP+M EV + LK
Sbjct:  206 GNLSCL---LHFDASQN----NLTGSIFPGITSLTNLLTLDLSSNSFEGTIPREIGQLENLELLILGKNDLTGRIPQEIGSLKQLKLLHLEECQFTGKIPWSISGLSSLTELDISDNNFDAELPSSMGE-LGNLTQLIAKNAGLSGNMPKELGNCKKLTVINLSFNALIGPIPEEFADLEAIVSFFVEGNKLSGRVPDWIQKWKNARSIRLGQNKFSGPLPVLPLQHLLSFAAE-----SNLLSGSIPSHICQANSLHSLLLHHNNLTGTIDEAFKGCTNLTELNLLDNHIHGEVPGYLAELPLVTLELSQNKFAGMLPAELWESKTLLEISLSNNEITGPIPESIGKLSVLQRLHIDNNLLEGPIPQ-SVGDLRNLT-NLSLRGNRLSGIIPLALFNCRKLATLDLSYNNLTGNIPSAISHLTLLDSLIL---SSNQLSGSIPAEICVGFENEAHPDSEF-------------------LQHHGLL-DLSYNQLTGQIPTSI-KNCAMVMVLNLQGNLLNGTIPVELGELTNLTSINLSFNEFVGPMLPWSGPLVQLQGLILSNNHLDGSIP-----------------AKIGQILPKIAVLDLSSNALTGTLPQSLLCNNYLNHLDVSNNHLSGHIQFSCPDGKEYSSTLLFFNSSSNHFSGSLDESISNFTQLSTLDIHNNSLTGRLPSALSDLSSLNYLDLSSN-NLYGAIPCGICNIFGLSFANFSGNYIDMYSLADCAAGGICSTNGTDHKALHPYHRVRRA-ITICAFTFVIIIVLVLLAVYLRRKLVRSRPLAFESASKAKATVEPTSTDELLGKKSREPLSINLATFEHALLRVTADDILKATENFSKVHIIGDGGFGTVYKAALPEGRRVAIKRLHGGHQFQGDREFLAEMETIGKVKHPNLVPLLGYCVCGDERFLIYEYMENGSLEMWLRNRADALEALGWPDRLKICLGSARGLAFLHHGFVPHIIHRDMKSSNILLDENFEPRVSDFGLARIISACETHVSTDIAGTFGYIPPEYGLTMKSTTKGDVYSFGVVMLELLTGRPPTGQ--EEVQGGGNLVGWVRWMIARGKQNELFDPCLPVSSVW----REQMARVLAIARDCTADEPFKRPTMLEVVKGLK 1276          

HSP 2 Score: 172.94 bits (437), Expect = 4.167e-42
Identity = 185/623 (29.70%), Postives = 278/623 (44.62%), Query Frame = 2

Query:  629 HVDVDDNISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIP------------------------AALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIP-VPCMVVFNISQNSLYGNISRFSHGECSNGSLNLSMSYMDLVGLYSSFFYRNALINVGSGHSPFSLSEFVVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKC-LKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIP 2419
            H  V  ++S V L       IG    L  L+     F GELP  +G L  L+ LDL  N   GPIP                         A+ +   L  +++S N ++G++P   G + + +++D   N  +G IP   G N   L+H   + N L+GSI   + +   L +L LSSN  +  IP  +G+LENLE L L +N L+G +P  +G  KQLK                   + H EE        F G++P SI  L  +  L + + N +   P + G   NL +L      L+G +P   GNCK L  ++LS N L G +P     +  +V F +  N L G +  +     +  S+ L                     N  SG  P    + ++      N  +GS+P  I    +L + L     L+ NN  G +   +F  C  L  L  +  +N I GE+P   G+  +  L  L L+ N+F G +P    +  +LL+++LS N + GPIP  IG++  ++ L + NN   G +P  +  L +L  L L  N LSG I   L   + L+ L L +N LTG IP   S++T L    +S N L+GSIP
Sbjct:   65 HNVVAIDLSSVPLYAPFPLCIGAFQSLVRLNFSGCGFSGELPEALGNLQNLQYLDLSNNELTGPIPISLYNLKMLKEMVLDYNSLSGQLSPAIAQLQHLTKLSISMNSISGSLPPDLGSLKNLELLDIKMNTFNGSIPATFG-NLSCLLHFDASQNNLTGSIFPGITSLTNLLTLDLSSNSFEGTIPREIGQLENLELLILGKNDLTGRIPQEIGSLKQLK-------------------LLHLEECQ------FTGKIPWSISGLSSLTELDISDNNFDAELPSSMGELGNLTQLIAKNAGLSGNMPKELGNCKKLTVINLSFNALIGPIPEEFADLEAIVSFFVEGNKLSGRVPDWIQKWKNARSIRLGQ-------------------NKFSGPLPVLPLQHLLSFAAESNLLSGSIPSHICQANSLHSLL-----LHHNNLTGTIDE-AFKGCTNLTEL--NLLDNHIHGEVP---GYLAELPLVTLELSQNKFAGMLPAELWESKTLLEISLSNNEITGPIPESIGKLSVLQRLHIDNNLLEGPIPQSVGDLRNLTNLSLRGNRLSGIIPLALFNCRKLATLDLSYNNLTGNIPSAISHLTLLDSLILSSNQLSGSIP 631          

HSP 3 Score: 93.9745 bits (232), Expect = 7.641e-18
Identity = 98/349 (28.08%), Postives = 146/349 (41.83%), Query Frame = 2

Query:  578 VKGDQVRGNFTL-LYPCLHVDVDDNISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGK------------LNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNK------------------------LSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYF 1513
            ++G+++ G   L L+ C  +   D +S+  L G +  AI HLT L  L L  N+  G +P E+              L    +LDL +N   G IP ++K C  + V+NL GN LNGTIP   G +T+   ++ SFN+                        L G IP ++G+    +  L L+SN L+G++P +L          L +N L +              LD+S N LSG +  +  D             G  +S   S+ +F          N F G L ESI     +  L + N +L G  P      S+L  L+++ N L G IP    N   L F
Sbjct:  573 LRGNRLSGIIPLALFNCRKLATLD-LSYNNLTGNIPSAISHLTLLDSLILSSNQLSGSIPAEICVGFENEAHPDSEFLQHHGLLDLSYNQLTGQIPTSIKNCAMVMVLNLQGNLLNGTIPVELGELTNLTSINLSFNEFVGPMLPWSGPLVQLQGLILSNNHLDGSIPAKIGQILPKIAVLDLSSNALTGTLPQSL----------LCNNYLNH--------------LDVSNNHLSGHIQFSCPD-------------GKEYS---STLLFFNS-----SSNHFSGSLDESISNFTQLSTLDIHNNSLTGRLPSALSDLSSLNYLDLSSNNLYGAIPCGICNIFGLSF 875          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy Swiss-Prot
Match: RCH2_ARATH (Receptor-like protein kinase 2 OS=Arabidopsis thaliana GN=RCH2 PE=1 SV=1)

HSP 1 Score: 354.369 bits (908), Expect = 3.814e-101
Identity = 313/1017 (30.78%), Postives = 492/1017 (48.38%), Query Frame = 2

Query:  650 ISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNK-LSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYG--------EGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNL-YFXXXXXXXXXXXXPAAIPVPCMVVFNISQNSLYGNISRFSHGECSNGSLNLSMSYMDLVGLYSSFFYRNALINVGSGHSPFSL---SEFVVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDX-XXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSI--SLNCESVKENPNLQPCHTD*SSSELERHHFGNISQGGRSPRENIQKIRSEFNQIEIASITSASVIFSVLIALIFFLVCMKKFACNAVSDHVSGRKEVVTCNSISIQLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLGRFQG---------VQQFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQERSRRTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSET-HATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWASMLLAQGRGCEFFMA---RLWDSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRLKAIQ 3613
            IS   L G L  ++G    L+VL L  N   G++P  + KL  LE L L  N   G IP  + KC+ L+ + L  N L G+IP   G ++  +V+    NK +SG+IP+E+G+ C +L  L LA   +SG++PS+LG   +L++L + + ++  +IPS LG    L  L L  N LSG +P  +G   +L+          LF  + S      EE G        +   N   G +P SI +L  +    + +    G  P T  +CS+L +L + +N ++G IP+  G    L  F   S+       P       +   ++S+NSL G I               S  +M L  L       N+L    SG  P  +   S  V L     N+ TG +P  I SL     K+N+    + N   G +       C  L   + D SNN + G LP N   +   L++L +++N+F G IP S   +VSL KL LS+N   G IP+ +G    ++ L L +N  SG +P +L    +    L LSSN L+G+I   +A L  LS L L HN L G +    +N+  L   N+S+N+ +G +P N +   L+ + ++ N  L  C +   S  L       +   G + R                 +T A +I   ++ +I   V + +   N  ++  S   E         Q    +V +   C    N IG G  G  Y+A+V  G V+AVK+L      G            F+AE+KTLG ++H N+V  +G   +     L+Y+Y+P G+L + + ER   +++W + ++I L  A  L +LH +C+P ++HRDIK +NIL+   F  Y++DFGLA+L+   +    +  VAG++GY+APEY  + ++++K+DVYSYGVVVL +L+ K+ +DP+      G ++V W    + Q RG    +    R        ++++VL  A++C   SP  RP+M++VA  LK I+
Sbjct:  112 ISGANLTGTLPESLGDCLGLKVLDLSSNGLVGDIPWSLSKLRNLETLILNSNQLTGKIPPDISKCSKLKSLILFDNLLTGSIPTELGKLSGLEVIRIGGNKEISGQIPSEIGD-CSNLTVLGLAETSVSGNLPSSLGKLKKLETLSIYTTMISGEIPSDLGNCSELVDLFLYENSLSGSIPREIGQLTKLEQ---------LFLWQNSLVGGIPEEIGNCSNLKMIDLSLNLLSGSIPSSIGRLSFLEEFMISDNKFSGSIPTTISNCSSLVQLQLDKNQISGLIPSELGTLTKLTLFFAWSNQLEGSIPPGLADCTDLQALDLSRNSLTGTIP--------------SGLFM-LRNLTKLLLISNSL----SGFIPQEIGNCSSLVRLR-LGFNRITGEIPSGIGSL----KKINF-LDFSSNRLHGKVPD-EIGSCSELQ--MIDLSNNSLEGSLP-NPVSSLSGLQVLDVSANQFSGKIPASLGRLVSLNKLILSKNLFSGSIPTSLGMCSGLQLLDLGSNELSGEIPSELGDIENLEIALNLSSNRLTGKIPSKIASLNKLSILDLSHNMLEGDLA-PLANIENLVSLNISYNSFSGYLPDNKLFRQLSPQDLEGNKKL--CSSTQDSCFLTYRKGNGLGDDGDASR------------TRKLRLTLALLITLTVVLMILGAVAVIRARRNIDNERDSELGETYKWQFTPFQKLNFSVDQIIRCLVEPNVIGKGCSGVVYRADVDNGEVIAVKKLWPAMVNGGHDEKTKNVRDSFSAEVKTLGTIRHKNIVRFLGCCWNRNTRLLMYDYMPNGSLGSLLHERRGSSLDWDLRYRILLGAAQGLAYLHHDCLPPIVHRDIKANNILIGLDFEPYIADFGLAKLVDEGDIGRCSNTVAGSYGYIAPEYGYSMKITEKSDVYSYGVVVLEVLTGKQPIDPTVPE---GIHLVDW----VRQNRGSLEVLDSTLRSRTEAEADEMMQVLGTALLCVNSSPDERPTMKDVAAMLKEIK 1067          

HSP 2 Score: 152.91 bits (385), Expect = 4.289e-36
Identity = 149/477 (31.24%), Postives = 226/477 (47.38%), Query Frame = 2

Query:  995 SLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWL-PNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIP-VPCMVVFNISQNSLYGNISRFSHGECSNGSLNLSMSYMDLVGLYSSFFYRNALINVGSGHSPFSLSEFVVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIP 2419
            SL  L ++   L+G++P +LG+C  LK L LSSN L  DIP SL KL NLE L L+ N L+G +PP +  C                S+  S  +F          N   G +P  + KL G+ V+ +  N  + G  P   G CSNL  L +A+  ++G +P+S G  K L  L + +  +SG +P+ +     +V   + +NSL G+I R                   L  L   F ++N+L  VG           + + D S N  +GS+P  I  L  L       F ++ N F G++   +   C  L  L  D   N+I G +P  +G   K L +    SN+  GSIP    D   L  L+LS N L G IPS +  ++ +  L L +N+ SG +P ++   SSL  L L  N ++GEI   +  LK ++ L    N+L G++P    + + L + ++S N+L GS+P
Sbjct:  106 SLQKLTISGANLTGTLPESLGDCLGLKVLDLSSNGLVGDIPWSLSKLRNLETLILNSNQLTGKIPPDISKC----------------SKLKSLILFD---------NLLTGSIPTELGKLSGLEVIRIGGNKEISGQIPSEIGDCSNLTVLGLAETSVSGNLPSSLGKLKKLETLSIYTTMISGEIPSDLGNCSELVDLFLYENSLSGSIPR---------------EIGQLTKLEQLFLWQNSL--VGGIPEEIGNCSNLKMIDLSLNLLSGSIPSSIGRLSFLEE-----FMISDNKFSGSIPT-TISNCSSLVQLQLD--KNQISGLIPSELGTLTK-LTLFFAWSNQLEGSIPPGLADCTDLQALDLSRNSLTGTIPSGLFMLRNLTKLLLISNSLSGFIPQEIGNCSSLVRLRLGFNRITGEIPSGIGSLKKINFLDFSSNRLHGKVPDEIGSCSELQMIDLSNNSLEGSLP 531          

HSP 3 Score: 90.1225 bits (222), Expect = 8.929e-17
Identity = 60/186 (32.26%), Postives = 99/186 (53.23%), Query Frame = 2

Query:  632 VDVDDNISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVP 1189
            +D+ +N     L G L   +  L+ L+VL +  N+F G++P  +G+L  L  L L  N F G IP +L  C+ L++++L  N+L+G IP+  G I + ++                         L+L+SN L+G IPS + +  +L  L LS N+L+ D+ + L  +ENL +L++S N  SG +P
Sbjct:  519 IDLSNN----SLEGSLPNPVSSLSGLQVLDVSANQFSGKIPASLGRLVSLNKLILSKNLFSGSIPTSLGMCSGLQLLDLGSNELSGEIPSELGDIENLEIA------------------------LNLSSNRLTGKIPSKIASLNKLSILDLSHNMLEGDL-APLANIENLVSLNISYNSFSGYLP 675          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy Swiss-Prot
Match: GSO2_ARATH (LRR receptor-like serine/threonine-protein kinase GSO2 OS=Arabidopsis thaliana GN=GSO2 PE=2 SV=2)

HSP 1 Score: 355.525 bits (911), Expect = 5.924e-101
Identity = 329/1063 (30.95%), Postives = 517/1063 (48.64%), Query Frame = 2

Query:  665 LRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFN------------------------KLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVL-WLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIP-VPCMVVFNISQNSLYGNI-------SRFSHGECSNGSLNLSMSYMDLVGLYSSFFYRNALINVGSGHSPFSLSEFVVLHDF--SRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPT---SFTDMVSLL---------------------KLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXX-XGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISLNCESVKENPNLQPCHTD*SSSELERHHFGNISQGGRSPRENIQKIRSEFNQIEIASITS-ASVIFSVLIALIFFLVCMKKFA------CNAVSDHVSGRKEVVTCNSISIQLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKR-LSLGRFQGVQQFAAEIKTLGRVQHPNLVTLIGYHVSEAE--MFLIYNYLPGGNLENFIQE----RSRRTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLG---TSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWASMLLAQGRGCEFFMARLWDSG-------PQHDLIEVLHLAVMCTGESPSSRPSMREVARRL 3601
            L G +   IG+ T L + +  FN   G LP E+ +L  L+ L+LG N+F G IP+ L    S++ +NL GNQL G IP     + + Q +D S N                        +LSG +P  +  N  SL  L L+  +LSG IP+ + NC  LK L LS+N L   IP SL +L  L  L L+ N L G +  ++ +   L+   L + N      K   F+   E     + N F GE+P  I     +  + W  N  L G  P + G   +L RL++ +N L G IPAS GNC  +  +DL+ N LSGS+P++   +  + +F I  NSL GN+          +    S+   N S+S   L G  SS+   +   N   G  P  L +   L      +NQFTG +P     +  LS        ++ N+  G + V     C +L  +  D +NN + G +P  +G     L  L L+SN+FVGS+PT   S T++++L                       LNL EN+L GP+PS IG++ ++  L LS N  +G +P ++ QL  L+     S +  +G I   ++ L  L +L L HN+L G++P    +M +L   N+S+NNL G +         ++   N  L  C +              +S   R+  +N Q+  S    + I++I+S A++   VL+ ++FF      F           S+  S +  + +       + +++++ AT   N +  IGSGG G  YKAE+  G  +AVK+ L        + F  E+KTLG ++H +LV L+GY  S+A+    LIY Y+  G++ +++      + +  + W    KIAL +A  + +LH +CVP ++HRDIK SN+LLD +  A+L DFGLA++L     + T + T  AG++GY+APEYA + + ++K+DVYS G+V++ +++ K    P+ + F    ++V W   +L    G E    +L DS         +    +VL +A+ CT   P  RPS R+ +  L
Sbjct:  204 LEGPIPAEIGNCTSLALFAAAFNRLNGSLPAELNRLKNLQTLNLGDNSFSGEIPSQLGDLVSIQYLNLIGNQLQGLIPKRLTELANLQTLDLSSNNLTGVIHEEFWRMNQLEFLVLAKNRLSGSLPKTICSNNTSLKQLFLSETQLSGEIPAEISNCQSLKLLDLSNNTLTGQIPDSLFQLVELTNLYLNNNSLEGTLSSSISNLTNLQEFTLYHNNLEGKVPKEIGFLGKLEIMYLYE-NRFSGEMPVEIGNCTRLQEIDWYGN-RLSGEIPSSIGRLKDLTRLHLRENELVGNIPASLGNCHQMTVIDLADNQLSGSIPSSFGFLTALELFMIYNNSLQGNLPDSLINLKNLTRINFSSNKFNGSIS--PLCG-SSSYLSFDVTENGFEGDIPLELGKSTNLDRLRLGKNQFTGRIPRTFGKISELSL-----LDISRNSLSGIIPV-ELGLCKKLTHI--DLNNNYLSGVIPTWLGK-LPLLGELKLSSNKFVGSLPTEIFSLTNILTLFLDGNSLNGSIPQEIGNLQALNALNLEENQLSGPLPSTIGKLSKLFELRLSRNALTGEIPVEIGQLQDLQSALDLSYNNFTGRIPSTISTLPKLESLDLSHNQLVGEVPGQIGDMKSLGYLNLSYNNLEGKLKKQFSRWQADAFVGNAGL--CGSP-------------LSHCNRAGSKN-QRSLSPKTVVIISAISSLAAIALMVLVIILFFKQNHDLFKKVRGGNSAFSSNSSSSQAPLFSNGGAKSDIKWDDIMEATHYLNEEFMIGSGGSGKVYKAELKNGETIAVKKILWKDDLMSNKSFNREVKTLGTIRHRHLVKLMGYCSSKADGLNLLIYEYMANGSVWDWLHANENTKKKEVLGWETRLKIALGLAQGVEYLHYDCVPPIVHRDIKSSNVLLDSNIEAHLGDFGLAKILTGNYDTNTESNTMFAGSYGYIAPEYAYSLKATEKSDVYSMGIVLMEIVTGKM---PTEAMFDEETDMVRWVETVLDTPPGSE-AREKLIDSELKSLLPCEEEAAYQVLEIALQCTKSYPQERPSSRQASEYL 1232          

HSP 2 Score: 178.718 bits (452), Expect = 5.979e-44
Identity = 201/678 (29.65%), Postives = 304/678 (44.84%), Query Frame = 2

Query:  647 NISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFG-------------------------ELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNF-------FEGELPESIV-KLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIP-VPCMVVFNISQNSLYGNI-----------------SRFSH------GECS------------NGSLNLSMSYM-DLVGLYSSFFYRNALINVGSGHSPFSLS--EFVVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGN--------------NFEGNLSV*SFDP-CLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIP 2419
            N+S +GL G +SP+IG    L  + L  N   G                         ++P ++G L  L+ L LG N  +G IP       +L+++ L+  +L G IP+ FG +   Q +    N+L G IP E+G NC SL     A N L+GS+P+ L     L++L L  N    +IPS LG L +++ L+L  N L GL+P  L +   L+ L          S    + + H+E +      F         G LP++I      +  L+L    L G  P    +C +LK L+++ N LTGQIP S      L  L L++N+L G+L ++I  +  +  F +  N+L G +                 +RFS       G C+            +G +  S+  + DL  L+      N L+    G+ P SL     + + D + NQ +GS+P     L  L   + Y   L GN              NF  N    S  P C     L FD + N   G++PL +G +   L  L L  N+F G IP +F  +  L  L++S N L G IP  +G  K++ ++ L+NN  SG +P  L +L  L  L+LSSN   G +  ++  L ++  L LD N L G IP    N+ AL+  N+  N L+G +P
Sbjct:   77 NLSGLGLTGSISPSIGRFNNLIHIDLSSNRLVGPIPTTLSNLSSSLESLHLFSNLLSGDIPSQLGSLVNLKSLKLGDNELNGTIPETFGNLVNLQMLALASCRLTGLIPSRFGRLVQLQTLILQDNELEGPIPAEIG-NCTSLALFAAAFNRLNGSLPAELNRLKNLQTLNLGDNSFSGEIPSQLGDLVSIQYLNLIGNQLQGLIPKRLTELANLQTLD--------LSSNNLTGVIHEEFWRMNQLEFLVLAKNRLSGSLPKTICSNNTSLKQLFLSETQLSGEIPAEISNCQSLKLLDLSNNTLTGQIPDSLFQLVELTNLYLNNNSLEGTLSSSISNLTNLQEFTLYHNNLEGKVPKEIGFLGKLEIMYLYENRFSGEMPVEIGNCTRLQEIDWYGNRLSGEIPSSIGRLKDLTRLH---LRENELV----GNIPASLGNCHQMTVIDLADNQLSGSIPSSFGFLTALELFMIYNNSLQGNLPDSLINLKNLTRINFSSNKFNGSISPLCGSSSYLSFDVTENGFEGDIPLELGKSTN-LDRLRLGKNQFTGRIPRTFGKISELSLLDISRNSLSGIIPVELGLCKKLTHIDLNNNYLSGVIPTWLGKLPLLGELKLSSNKFVGSLPTEIFSLTNILTLFLDGNSLNGSIPQEIGNLQALNALNLEENQLSGPLP 737          

HSP 3 Score: 144.05 bits (362), Expect = 2.894e-33
Identity = 184/581 (31.67%), Postives = 262/581 (45.09%), Query Frame = 2

Query:  860 INLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXX--XXXXXXXGPL----------------FSRKGSSF------MFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIPV--PCMVVFNISQNSLYGNI-------SRFSHGECSNGSL--NLSMSYMDLVGLYSSFFYRNALINVGSGHSPFSLSEFVVLHDFS--RNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILP-----------------------DLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPL 2422
            +NLSG  L G+I    G   +   +D S N+L G IP  L     SL  LHL SN LSG IPS LG+   LKSL L  N L   IP + G L NL+ L L+   L+GL+P   G   QL+   L      GP+                F+R   S       + + +    GD N F GE+P  +  L  I  L L    L+G+ P+     +NL+ L+++ N LTG I   F     L FL L+ N LSGSLP  I      +    +S+  L G I             + SN +L   +  S   LV L + +   N+L     G    S+S    L +F+   N   G VP  I  L  L        +L  N F G + V     C RL  +  D   N++ GE+P ++G   K L  L L  NE VG+IP S  +   +  ++L++N+L G IPS  G +  +    + NN+  G +P  L+ L +L  +  SSN  +G I P                       +L +  +L  LRL  N+ TG+IP  F  ++ LS+ ++S N+L+G IP+
Sbjct:   76 LNLSGLGLTGSISPSIGRFNNLIHIDLSSNRLVGPIPTTLSNLSSSLESLHLFSNLLSGDIPSQLGSLVNLKSLKLGDNELNGTIPETFGNLVNLQMLALASCRLTGLIPSRFGRLVQLQTLILQDNELEGPIPAEIGNCTSLALFAAAFNRLNGSLPAELNRLKNLQTLNLGD-NSFSGEIPSQLGDLVSIQYLNLIGNQLQGLIPKRLTELANLQTLDLSSNNLTGVIHEEFWRMNQLEFLVLAKNRLSGSLPKTICSNNTSLKQLFLSETQLSGEIPAEISNCQSLKLLDLSNNTLTGQIPDSLFQLVELTNLYLNNNSL----EGTLSSSISNLTNLQEFTLYHNNLEGKVPKEIGFLGKLEI-----MYLYENRFSGEMPV-EIGNCTRLQEI--DWYGNRLSGEIPSSIGR-LKDLTRLHLRENELVGNIPASLGNCHQMTVIDLADNQLSGSIPSSFGFLTALELFMIYNNSLQGNLPDSLINLKNLTRINFSSNKFNGSISPLCGSSSYLSFDVTENGFEGDIPLELGKSTNLDRLRLGKNQFTGRIPRTFGKISELSLLDISRNSLSGIIPV 642          

HSP 4 Score: 122.094 bits (305), Expect = 1.571e-26
Identity = 93/288 (32.29%), Postives = 136/288 (47.22%), Query Frame = 2

Query:  626 LHVDVDDNISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASF 1489
            L  DV +N    G  G +   +G  T L  L LG N+F G +P   GK++ L +LD+  N+  G IP  L  C  L  I+L+ N L+G IP   G +     +  S NK  G +P E+  +  +++ L L  N L+GSIP  +GN   L +L L  N L   +PS++GKL  L  L LSRN L+G +P  +G  + L+  +                        +  YN F G +P +I  L  +  L L +  L G  P   G   +L  LN++ N L G++   F
Sbjct:  579 LSFDVTEN----GFEGDIPLELGKSTNLDRLRLGKNQFTGRIPRTFGKISELSLLDISRNSLSGIIPVELGLCKKLTHIDLNNNYLSGVIPTWLGKLPLLGELKLSSNKFVGSLPTEIF-SLTNILTLFLDGNSLNGSIPQEIGNLQALNALNLEENQLSGPLPSTIGKLSKLFELRLSRNALTGEIPVEIGQLQDLQSAL------------------------DLSYNNFTGRIPSTISTLPKLESLDLSHNQLVGEVPGQIGDMKSLGYLNLSYNNLEGKLKKQF 837          

HSP 5 Score: 118.627 bits (296), Expect = 1.802e-25
Identity = 147/512 (28.71%), Postives = 231/512 (45.12%), Query Frame = 2

Query:  971 NELGENCG--SLMHLHLASNELSGSIPSNLGNCGELKSLILSSNIL-------------------------QNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAI-PVPCMVVFNISQNSLYGNISRFSHGECSNGSLNLSMSYMDLVGLYSSFFYR-NALINVGSGHSPFSLSEFVVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIP 2419
            N  G  CG   ++ L+L+   L+GSI  ++G    L  + LSSN L                           DIPS LG L NL++L L  N L+G +P T G+   L++L L +                             G +P    +L  +  L L +  LEG  P   G+C++L     A N L G +PA     KNL  L+L  N+ SG +P+ +  +  +   N+  N L G I +    E +N    L +S  +L G+    F+R N L             EF+VL   ++N+ +GS+P  I S  T   +L    +L+     G +       C  L   + D SNN + G++P ++    + L  L L +N   G++ +S +++ +L +  L  N L G +P  IG + ++  + L  N FSG MP ++   + L+ ++   N LSGEI   +  LK L+ L L  N+L G IP    N   ++V +++ N L+GSIP
Sbjct:   62 NWTGVTCGGREIIGLNLSGLGLTGSISPSIGRFNNLIHIDLSSNRLVGPIPTTLSNLSSSLESLHLFSNLLSGDIPSQLGSLVNLKSLKLGDNELNGTIPETFGNLVNLQMLALASCR-------------------------LTGLIPSRFGRLVQLQTLILQDNELEGPIPAEIGNCTSLALFAAAFNRLNGSLPAELNRLKNLQTLNLGDNSFSGEIPSQLGDLVSIQYLNLIGNQLQGLIPK-RLTELANLQ-TLDLSSNNLTGVIHEEFWRMNQL-------------EFLVL---AKNRLSGSLPKTICSNNTSLKQL----FLSETQLSGEIPA-EISNCQSLK--LLDLSNNTLTGQIPDSLFQLVE-LTNLYLNNNSLEGTLSSSISNLTNLQEFTLYHNNLEGKVPKEIGFLGKLEIMYLYENRFSGEMPVEIGNCTRLQEIDWYGNRLSGEIPSSIGRLKDLTRLHLRENELVGNIPASLGNCHQMTVIDLADNQLSGSIP 522          

HSP 6 Score: 70.0922 bits (170), Expect = 1.309e-10
Identity = 70/246 (28.46%), Postives = 103/246 (41.87%), Query Frame = 2

Query: 1727 NVGSGHSPFSLSEFVVLHDFS--RNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISLNCESVKE 2458
            N+ SG  P  L   V L       N+  G++P    +L  L         L G      L    F   ++L  LI    +N++ G +P  +G+ C  L + + A N   GS+P     + +L  LNL +N   G IPS +G++  I+YL+L  N   G +P                          L EL +L  L L  N LTG I   F  M  L    ++ N L+GS+P  +I  N  S+K+
Sbjct:  130 NLLSGDIPSQLGSLVNLKSLKLGDNELNGTIPETFGNLVNLQMLALASCRLTG------LIPSRFGRLVQLQTLILQ--DNELEGPIPAEIGN-CTSLALFAAAFNRLNGSLPAELNRLKNLQTLNLGDNSFSGEIPSQLGDLVSIQYLNLIGNQLQGLIP------------------------KRLTELANLQTLDLSSNNLTGVIHEEFWRMNQLEFLVLAKNRLSGSLP-KTICSNNTSLKQ 341          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy Swiss-Prot
Match: RPK1_IPONI (Receptor-like protein kinase OS=Ipomoea nil GN=INRPK1 PE=2 SV=2)

HSP 1 Score: 352.829 bits (904), Expect = 8.200e-101
Identity = 338/1053 (32.10%), Postives = 491/1053 (46.63%), Query Frame = 2

Query:  647 NISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAI-PVPCMVVFNISQNSLYGNISRFSHGECSNGSLNLSMSYMDLVGLYSSFFYRNALINVGSGHSPFSL---SEFVVLHDFSRNQFTGSVPPFIISLYTLSAKL---NY----------------GFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSEN------------------------RL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISLNCESVKENPNL-----QPCHTD*SSSELERHHFGNI-------SQGGRSPRENIQKIRSEFNQIEIASITSASVIFSVLIALIFFLVCMKKFACNAVSDHVSGRKEVVTCNSISIQLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSL-GRFQGVQQFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQERSR-RTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTD-VAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNG-FNIVTWASMLLAQGRGCE-----FFMARLWDSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRL 3601
            N+S  G+ G   P I HL  L+ + L  N FFG +P ++G  +LLE +DL  N+F G IP  L    +LR ++L  N L G  P     I   + V F+ N L+G IP+ +G N   L  L L  N+ SG +PS+LGN   L+ L L+ N L   +P +L  LENL  LD+  N L G +P     CKQ+  + L N         G        E+G        G +P    +L  +  L+L   +  G  P   G C ++  L + QN L G+IP   G    L +L L +NNLSG +P +I  +  +    + QN+L G                L +   +L  L S   Y N      +G  P  L   S   VL D +RN FTG +PP + S   L   L   NY                   L  NN  G L     D   + + L FD S N   G +P ++G+  K +  + L+SN+  GSIP     +V L  LNLS N                         L G IPS +G + E+  LSL  N+FSG +P  L Q + L  L+L  N L+G+I P +  L+ L +L L  NKL GQ+P     +  L   +VS NNL+G++ + S   +   +  + NL      P  T   +S       GN        + G   P  +I +  +  +      +++  +   VL AL+F ++C+  F+           +E+              V+ AT   N +  IG G  G  YKA + P  V AVK+L   G   G      EI+T+G+V+H NL+ L  + + +    ++Y Y+  G+L + + E +  + ++W   H IA+  A  L +LH +C P ++HRDIKP NILLD     ++SDFG+A+LL  S T   ++ V GT GY+APE A T   S ++DVYSYGVV+L L++ KKALDPSF    NG  +IV W   +  Q    +       +  L DS     + E L LA+ C  +    RP+MR+V ++L
Sbjct:   74 NLSSYGISGEFGPEISHLKHLKKVVLSGNGFFGSIPSQLGNCSLLEHIDLSSNSFTGNIPDTLGALQNLRNLSLFFNSLIGPFPESLLSIPHLETVYFTGNGLNGSIPSNIG-NMSELTTLWLDDNQFSGPVPSSLGNITTLQELYLNDNNLVGTLPVTLNNLENLVYLDVRNNSLVGAIPLDFVSCKQIDTISLSNNQFTGGLPPGLGNCTSLREFGAFSC-ALSGPIPSCFGQLTKLDTLYLAGNHFSGRIPPELGKCKSMIDLQLQQNQLEGEIPGELGMLSQLQYLHLYTNNLSGEVPLSIWKIQSLQSLQLYQNNLSGE---------------LPVDMTELKQLVSLALYENHF----TGVIPQDLGANSSLEVL-DLTRNMFTGHIPPNLCSQKKLKRLLLGYNYLEGSVPSDLGGCSTLERLILEENNLRGGLP----DFVEKQNLLFFDLSGNNFTGPIPPSLGN-LKNVTAIYLSSNQLSGSIPPELGSLVKLEHLNLSHNILKGILPSELSNCHKLSELDASHNLLNGSIPSTLGSLTELTKLSLGENSFSGGIPTSLFQSNKLLNLQLGGNLLAGDI-PPVGALQALRSLNLSSNKLNGQLPIDLGKLKMLEELDVSHNNLSGTLRVLSTIQSLTFINISHNLFSGPVPPSLTKFLNSS-PTSFSGNSDLCINCPADGLACPESSILRPCNMQSNTGKGGLSTLGIAMIVLGALLF-IICLFLFSAFLFLHCKKSVQEIAISAQEGDGSLLNKVLEATENLNDKYVIGKGAHGTIYKATLSPDKVYAVKKLVFTGIKNGSVSMVREIETIGKVRHRNLIKLEEFWLRKEYGLILYTYMENGSLHDILHETNPPKPLDWSTRHNIAVGTAHGLAYLHFDCDPAIVHRDIKPMNILLDSDLEPHISDFGIAKLLDQSATSIPSNTVQGTIGYMAPENAFTTVKSRESDVYSYGVVLLELITRKKALDPSF----NGETDIVGWVRSVWTQTGEIQKIVDPSLLDELIDSSVMEQVTEALSLALRCAEKEVDKRPTMRDVVKQL 1092          

HSP 2 Score: 103.605 bits (257), Expect = 7.121e-21
Identity = 109/376 (28.99%), Postives = 171/376 (45.48%), Query Frame = 2

Query: 1361 IHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIPVPCMVVFNISQNSLYGNISRFSHGECSNGSLNLSMSYMDLVG-----------LYSSFFYRNAL-----INVGSGHSPFSLSEFVVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIF-DASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISL 2437
            +  L L +  + G F        +LK++ ++ N   G IP+  GNC  L                          ++S NS  GNI   + G   N   NLS+ +  L+G           L + +F  N L      N+G      ++SE   L     NQF+G VP  + ++ TL        +LN NN  G L V        L+ L++ D  NN +VG +PL+   +CK +  +SL++N+F G +P    +  SL +       L GPIPS  G++ ++  L L+ N+FSG +P +L +  S+  L+L  N L GEI  +L  L  L  L L  N L+G++P     + +L    +  NNL+G +P++   L
Sbjct:   70 VDTLNLSSYGISGEFGPEISHLKHLKKVVLSGNGFFGSIPSQLGNCSLLEH-----------------------IDLSSNSFTGNIPD-TLGALQNLR-NLSLFFNSLIGPFPESLLSIPHLETVYFTGNGLNGSIPSNIG------NMSELTTLW-LDDNQFSGPVPSSLGNITTLQE-----LYLNDNNLVGTLPV----TLNNLENLVYLDVRNNSLVGAIPLDF-VSCKQIDTISLSNNQFTGGLPPGLGNCTSLREFGAFSCALSGPIPSCFGQLTKLDTLYLAGNHFSGRIPPELGKCKSMIDLQLQQNQLEGEIPGELGMLSQLQYLHLYTNNLSGEVPLSIWKIQSLQSLQLYQNNLSGELPVDMTEL 403          

HSP 3 Score: 94.7449 bits (234), Expect = 3.899e-18
Identity = 128/454 (28.19%), Postives = 198/454 (43.61%), Query Frame = 2

Query: 1070 LKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIPVPCMVVFNISQNSLYGNISRFSHGECSNGSL--NLSMSYMDLVGLYSSFFYRNALINVGSGHSPFSLSEFVVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLN 2425
            + +L LSS  +  +    +  L++L+ + LS N   G +P  LG+C  L                         E+ +   N F G +P+++  L  +  L L   +L G FP++  S  +L+ +    N L G IP++ GN   L  L L  N  SG +P+++                GNI+       ++ +L   L ++  +L  L       N+L  VG+    F   + +     S NQFTG +PP +                 GN             C  L    F A +  + G +P   G   K L  L LA N F G IP       S++ L L +N+L G IP  +G + +++YL L  NN SG +P  + ++ SL+ L+L  N+LSGE+  D+ ELK L +L L  N  TG IP      ++L V +++ N  TG IP N
Sbjct:   70 VDTLNLSSYGISGEFGPEISHLKHLKKVVLSGNGFFGSIPSQLGNCSLL-------------------------EHIDLSSNSFTGNIPDTLGALQNLRNLSLFFNSLIGPFPESLLSIPHLETVYFTGNGLNGSIPSNIGNMSELTTLWLDDNQFSGPVPSSL----------------GNITTLQELYLNDNNLVGTLPVTLNNLENLVYLDVRNNSL--VGAIPLDFVSCKQIDTISLSNNQFTGGLPPGL-----------------GN-------------CTSLRE--FGAFSCALSGPIPSCFGQLTK-LDTLYLAGNHFSGRIPPELGKCKSMIDLQLQQNQLEGEIPGELGMLSQLQYLHLYTNNLSGEVPLSIWKIQSLQSLQLYQNNLSGELPVDMTELKQLVSLALYENHFTGVIPQDLGANSSLEVLDLTRNMFTGHIPPN 447          

HSP 4 Score: 60.077 bits (144), Expect = 1.411e-7
Identity = 58/180 (32.22%), Postives = 89/180 (49.44%), Query Frame = 2

Query:  629 HVDVDDNISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRN 1168
            H+++  NI    L+G L   + +  KL  L    N   G +P  +G L  L  L LG N+F G IP +L +   L  + L GN L G IP + G + + + ++ S NKL+G++P +LG+    L  L ++ N LSG++   L     L  + +S N+    +P SL K  N      S N
Sbjct:  551 HLNLSHNI----LKGILPSELSNCHKLSELDASHNLLNGSIPSTLGSLTELTKLSLGENSFSGGIPTSLFQSNKLLNLQLGGNLLAGDIPPV-GALQALRSLNLSSNKLNGQLPIDLGK-LKMLEELDVSHNNLSGTL-RVLSTIQSLTFINISHNLFSGPVPPSLTKFLNSSPTSFSGN 723          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy TrEMBL
Match: G5EM40_LOTJA (Leucine-rich repeat receptor-like kinase OS=Lotus japonicus GN=KLAVIER PE=4 SV=1)

HSP 1 Score: 897.116 bits (2317), Expect = 0.000e+0
Identity = 547/1089 (50.23%), Postives = 699/1089 (64.19%), Query Frame = 2

Query:  458 CLWHGVSCDPLEGRVITXXXXXXXXXTCSVLQLSASKTAEVKGDQVRGNFTLLYPCLHVDVDDNISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDL------------------------GFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPL--FSRKGSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIPVPCMVVFNISQNSLYGNISRFSHGEC-SNGSLNLSMSYMDLVGLYSSFFYRNALINVGSGHSPFSLSEFV---VLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISLNCESVKENPNLQPC-HTD*SSSELERHHFGNI--SQGGRSPRENIQKIRSEFNQIEIASITSASVIFSVLIALIFFLVCMKKFACNAVSDHV-SGRKEVVTCNSISIQLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLGRFQGVQQFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQERSRRTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWASMLLAQGRGCEFFMARLWDSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRLKAIQPPT 3622
            C W GVSCDP   RV+ +N++ N  +     +   S  ++     + G F +   C+        S   L G++SP    LT+LR+LSL FN F G +P E+  +N LEV+DL                        GFN   G +P +L    SL ++NL+GN +NG++P   G +     V  SFN L+G IP E+G++CG L HL L+ N L+  IP++LGNC +L+++ L SNILQ+ IP+ LGKL  LE LD+SRN L GLVPP LG C +L +LVL N   PL   S      +  Q      +YN+FEG +P  I+ L  + +LW P  NLE  FP++W +C NL+ LN+AQN  TG  P     CK L+FLDLS  NL+G L   +P PCM VF++S N L G+I  FS   C S  S N ++   D   L   FF+   ++      SP S    V   V+H+F +N F  S+    I+ Y L     Y   +  NN  G      F+ C  L+ L+ + S  +I G++  N G  CK LK L  + N+  G+IP    DMVSL+ LNLS N L G IP+ +G++ ++++LSL NNNFSG++P  L QL SLEVL+LSSNS  GEI   +  L++L+ + L++NKL+GQIP G +N++ LS FNVSFNNL+GS+P NS  + C S   NP L+ C     +    ++H   +   S     P +  +   + F  IEIA ITSAS I SVL+ALI   VC +K+  N  S  V S RKEV     +   LT+E+VVRATG FN  NCIG+GGFGATYKAE+ PG +VA+KRLS+GRFQG QQF AEIKTLGR+ HPNLVTLIGYH S++EMFLIYNYL GGNLE FIQERS R V+WR+LHKIALDIA AL +LHD+CVPRVLHRD+KPSNILLD  +NAYLSDFGLARLLGTSETHATT VAGTFGYVAPEYAMTCRVSDKADVYSYGVV+L LLSDKKALDPSFSS+GNGFNIV WA MLL QG+  +FF A LWD+ P  DL+EVLHLAV+CT E+ S+RP+M++V RRLK +QPP+
Sbjct:   71 CAWFGVSCDPSSHRVVAINVTGNGGN-----RKHPSPCSDFTEFPLYG-FGIRRSCVG-------SGGALFGKVSPLFSKLTELRILSLPFNGFEGVIPDEIWGMNKLEVIDLEGNLISGYLPSRFSGLRSLRVLNLGFNRIVGEVPNSLSSVASLEILNLAGNGINGSVPGFVGRLRG---VYLSFNLLTGSIPQEIGDDCGRLEHLDLSGNFLTLEIPNSLGNCSQLRTISLHSNILQDVIPAELGKLRKLEVLDVSRNTLGGLVPPELGHCMELSVLVLSNLFNPLPDVSGMARDSLTDQLVSVIDEYNYFEGPIPVEIMNLPKLKILWAPRANLEDSFPRSWNACGNLEMLNLAQNDFTGDFPNQLSRCKKLHFLDLSFTNLTGKLAKDLPAPCMTVFDVSGNVLSGSIPEFSGNACPSAPSWNGNLFESDNRALPYGFFFALKVLQ----RSPLSSLGDVGRSVIHNFGQNNFI-SMDSLPIARYRLGKGFAYAILVGENNLTGPFPTNLFEKCDGLNALLLNVSYTRISGQISSNFGRMCKSLKFLDASGNQITGTIPFDLGDMVSLVALNLSRNHLQGQIPTSLGQLNDLKFLSLGNNNFSGSIPTSLDQLHSLEVLDLSSNSFIGEIPKGIENLRNLTVVLLNNNKLSGQIPAGLANVSTLSAFNVSFNNLSGSLPSNSSLIKCSSAVGNPFLRSCIGVSLTVPSADQHGVADYPNSYTAAPPEDTGKTSGNGFTSIEIACITSASAIVSVLLALIVLFVCTRKW--NPRSRVVGSTRKEVTVFTDVGFPLTFESVVRATGSFNAGNCIGNGGFGATYKAEISPGNLVAIKRLSVGRFQGAQQFHAEIKTLGRLHHPNLVTLIGYHASDSEMFLIYNYLSGGNLEKFIQERSTRAVDWRILHKIALDIARALAYLHDQCVPRVLHRDVKPSNILLDDDYNAYLSDFGLARLLGTSETHATTGVAGTFGYVAPEYAMTCRVSDKADVYSYGVVLLELLSDKKALDPSFSSYGNGFNIVAWACMLLRQGQAKDFFTAGLWDAAPADDLVEVLHLAVVCTVETLSTRPTMKQVVRRLKQLQPPS 1136          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy TrEMBL
Match: A0A096Q101_MAIZE (Uncharacterized protein OS=Zea mays GN=Zm.107425 PE=4 SV=1)

HSP 1 Score: 895.96 bits (2314), Expect = 0.000e+0
Identity = 515/993 (51.86%), Postives = 663/993 (66.77%), Query Frame = 2

Query:  662 GLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELG-ENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGE-GDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIPVPCMVVFNISQNSLYGNISRF-SHGECSNGSLNLSMSYMDLVGLYSSFFYRNALINVGSGHSPFSLSEFVVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISLNCESVKENPNLQPCH----TD*SSSELERHHFGNISQGGRSPRENIQKIRSEFNQIEIASITSASVIFSVLIALIFFLVCMKKFACNAVSDHVSGRKEVVTCNSISIQLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLGRFQGVQQFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQERSRRTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWASMLLAQGRGCEFFMARLWDSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRLKAIQPP 3619
             L G LSPA+  L +LRVL+L  +   G LP  +  L  L VLDL  N   G IPA L  C SL+ ++L+ NQLNG++PA  G +   + +  + N+  G IP+ELG   C +L  L ++ N L G IP +LGNC EL++L+LSSN L + IP  +G+L+NL ALD+SRN LSG VP  LG C QL +LVL N   P      S       +YGE  D+N+F+G +P++I  L  + +LW P   LEG  P  W SC +L+ +N+ +N  +G IP     C+NL FL+LS N  +GS+ +++PVPCM VF++S N L G++  F S   C    L+      DLV  YSSFF   AL    S  SP   +     H FSRN FTG V    ++   L  + +Y F  +GN+  G L    FD C    GL+ + SNN I G +P ++G  C  L +L +A N+  G IP+S  ++  L+ L+LS NRL G IP+ +  +  ++ LSL+ N  +G +P D+ QL +L+VL+LSSN L G I   LA+L++L+AL LD+NKLTG+IP GF+N  +L+ FNVSFNNL+G +P N  ++ C+SV  NP LQ CH       S+++  R    N S          +   + FN IEIASITSA+ I S+L+ALI   +  +K A   +S   SGR+EV     I + +TYE VVRATG FN  NCIGSGGFGATYKAE+ PGV+VA+KRLS+GRFQG QQF AEIKTLGR++HPNLVTL+GYH+ E+EMFLIYNYL GGNLE FIQERS+R V+W+MLHKIALD+A AL +LHD CVPR+LHRD+KPSNILLD ++ AYLSDFGLARLLG SETHATT VAGTFGYVAPEYAMTCRVSDKADVYSYGVV++ L+SDKKALDPSFS +GNGFNIV WA MLL QGR  EFF+  LWD GP  DL+E LHLAV+CT +S S RP+M++V +RLK +QPP
Sbjct:   81 ALAGALSPAVAALRELRVLALPSHALSGPLPPAIWTLRRLRVLDLSGNRLQGGIPAVLV-CVSLQTLDLAYNQLNGSVPAALGALPVLRRLSLACNRFGGAIPDELGGAGCRNLQFLDVSGNMLVGGIPRSLGNCTELQALLLSSNNLDDIIPPEIGRLKNLRALDVSRNSLSGPVPAELGGCIQLSVLVLSNPYAPTAGSDSS-------DYGELDDFNYFQGGIPDTIATLPKLRMLWAPRATLEGELPGNWSSCQSLEMINLGENLFSGGIPKGLVECENLKFLNLSMNKFTGSVDSSLPVPCMDVFDVSGNQLSGSLPVFMSKKNC----LSSQAPRDDLVSEYSSFFTYQALAGFMSSPSPLD-AHLTSYHSFSRNNFTGPVTSLPLATEKLGMQGSYAFLADGNHLGGQLQPSLFDKCNSSRGLVVEISNNLISGAIPTDIGSLCSSLLVLGVAGNQLSGMIPSSIGELSYLISLDLSRNRLGGVIPTSVKNLLHLQRLSLAQNLLNGTIPPDINQLHALKVLDLSSNLLMGMIPDALADLRNLTALLLDNNKLTGKIPSGFANSASLTTFNVSFNNLSGPVPTNGNTVRCDSVIGNPLLQSCHVYTLAVPSAAQQGRGLNSNDSNDTTPSNSQNEGANNSFNAIEIASITSATAIVSILLALIALFIYTRKCAPR-MSARSSGRREVTLFQDIGVPITYETVVRATGSFNASNCIGSGGFGATYKAEIAPGVLVAIKRLSVGRFQGAQQFDAEIKTLGRLRHPNLVTLVGYHLGESEMFLIYNYLSGGNLERFIQERSKRPVDWKMLHKIALDVAKALAYLHDTCVPRILHRDVKPSNILLDTNYTAYLSDFGLARLLGNSETHATTGVAGTFGYVAPEYAMTCRVSDKADVYSYGVVLMELISDKKALDPSFSPYGNGFNIVAWACMLLRQGRAREFFIDGLWDVGPHDDLVETLHLAVICTADSLSIRPTMKQVVQRLKQLQPP 1059          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy TrEMBL
Match: K7MUY2_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 892.878 bits (2306), Expect = 0.000e+0
Identity = 539/1022 (52.74%), Postives = 676/1022 (66.14%), Query Frame = 2

Query:  662 GLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDL------------------------GFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRKGSSFMFHQEEYGE---GDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIPVPCMVVFNISQNSLYGNISRFSHGECS---NGSLNLSMSYMDLVGLYSSFFYRNALINVGSGHSPFSLSEF--VVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISLNCESVKENPNLQPCH-TD*SSSELERHHFGNISQGGRSPRENI-QKIRSEFNQIEIASITSASVIFSVLIALIFFLVCMKKFACNAVSDHV-SGRKEVVTCNSISIQLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLGRFQGVQQFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQERSRRTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWASMLLAQGRGCEFFMARLWDSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRLKAIQPPT 3622
             L G+LSP +  LT+LRVLSL FN+  GE+P E+  +  LEVLDL                        GFN   G IP++L    SL V+NL+GN +NG++P+  G +     V  S+N L G IP E+GE+CG L HL L+ N L  +IP +LGNC EL+ ++L SN L++ IP+ LG+L  LE LD+SRN L G VP  LG+C +L +LVL N    +    G+      E+       ++N+FEG +P  I+ L  + VLW P  NL G FP +WG C +L+ LN+AQN LTG  P   G CKNL+FLDLS+NN +G L   +PVPCM VF++S N L G I +FS G C+   + S NL     D    Y SFF    L     G    SL E    V H+F +N F  S+    I+   L   L Y   +  N   G      F+ C  L+ L+ + S   I G++P   G  C+ LK L  + N+  G IP    DMVSL+ LNLS+NRL   IP  +G++K++++LSL+ NN SG++P  L QL SLEVL+LSSNSL+GEI   +  L++L+ + L++NKL+GQIP G +N++ LS FNVSFNNL+GS+P N  S+ C +   NP L  C+    +    ++    N S    +P E   +K  + FN IEIASITSAS I SVL+ALI   +  +K+  N  S  V S RKEV     I + LT+ENVVRATG FN  NCIG+GGFGATYKAE+VPG +VA+KRL++GRFQG QQF AEIKTLGR++HPNLVTLIGYH SE EMFLIYNYLPGGNLE FIQERS R  +WR+LHKIALDIA AL +LHD+CVPRVLHRD+KPSNILLD  +NAYLSDFGLARLLGTSETHATT VAGTFGYVAPEYAMTCRVSDKADVYSYGVV+L LLSDKKALDPSFSS+GNGFNIV WA MLL QG+  EFF   LWD+GP+ DL+EVLHLAV+CT +S S+RPSM+ V RRLK +QPP+
Sbjct:  125 ALFGKLSPKLSELTELRVLSLPFNDLEGEIPEEIWGMEKLEVLDLEGNLISGVLPLRFNGLKNLKVLNLGFNRIVGEIPSSLSSFKSLEVLNLAGNGINGSVPSFVGRLRG---VYLSYNLLGGAIPQEIGEHCGQLDHLDLSGNLLMQAIPGSLGNCSELRMILLHSNSLEDVIPAELGRLRKLEVLDVSRNTLGGQVPMELGNCTELSVLVLSNLFSSVPDVNGTVRDLGVEQMVSMNIDEFNYFEGPVPVEIMNLPKLRVLWAPRANLAGSFPSSWGKCDSLEMLNLAQNDLTGDFPNQLGGCKNLHFLDLSANNFTGVLAEELPVPCMTVFDVSGNVLSGPIPQFSVGLCALVPSWSGNL-FETDDRALPYKSFFVSKIL----GGTILSSLGEVGRSVFHNFGQNNFV-SMESLPIARDRLGKGLAYAILVGENKLAGPFPTNLFEKCDGLNALLLNVSYTMISGQIPSKFGGMCRSLKFLDASGNQITGPIPVGLGDMVSLVSLNLSKNRLQDQIPGNLGQLKDLKFLSLAENNLSGSIPTSLGQLYSLEVLDLSSNSLTGEIPKGIENLRNLTDVLLNNNKLSGQIPAGLANVSTLSAFNVSFNNLSGSLPSNGNSIKCSNAVGNPFLHSCNEVSLAVPSADQGQVDNSSSYTAAPPEVTGKKGGNGFNSIEIASITSASAIVSVLLALIVLFIYTRKW--NPRSRVVGSTRKEVTVFTDIGVPLTFENVVRATGNFNASNCIGNGGFGATYKAEIVPGNLVAIKRLAVGRFQGAQQFHAEIKTLGRLRHPNLVTLIGYHASETEMFLIYNYLPGGNLEKFIQERSTRAADWRILHKIALDIARALAYLHDQCVPRVLHRDVKPSNILLDDDYNAYLSDFGLARLLGTSETHATTGVAGTFGYVAPEYAMTCRVSDKADVYSYGVVLLELLSDKKALDPSFSSYGNGFNIVAWACMLLRQGQAKEFFATGLWDTGPEDDLVEVLHLAVVCTVDSLSTRPSMKHVVRRLKQLQPPS 1135          

HSP 2 Score: 72.0182 bits (175), Expect = 2.773e-9
Identity = 53/165 (32.12%), Postives = 86/165 (52.12%), Query Frame = 2

Query:  566 KTAEVKGDQVRGNFTLLYPCLHVDVDDNISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIP-NELGENCGSLM---HLHLASNELSGSIPS 1048
            K  +  G+Q+ G   +    +   V  N+S   L+ ++   +G L  L+ LSL  N   G +P  +G+L  LEVLDL  N+  G IP  ++   +L  + L+ N+L+G IPA    +++    + SFN LSG +P N     C + +    LH + NE+S ++PS
Sbjct:  599 KFLDASGNQITGPIPVGLGDMVSLVSLNLSKNRLQDQIPGNLGQLKDLKFLSLAENNLSGSIPTSLGQLYSLEVLDLSSNSLTGEIPKGIENLRNLTDVLLNNNKLSGQIPAGLANVSTLSAFNVSFNNLSGSLPSNGNSIKCSNAVGNPFLH-SCNEVSLAVPS 762          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy TrEMBL
Match: A0A0B2Q232_GLYSO (LRR receptor-like serine/threonine-protein kinase RPK2 OS=Glycine soja GN=glysoja_038099 PE=4 SV=1)

HSP 1 Score: 890.952 bits (2301), Expect = 0.000e+0
Identity = 541/1024 (52.83%), Postives = 680/1024 (66.41%), Query Frame = 2

Query:  665 LRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDL------------------------GFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRK-------GSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIPVPCMVVFNISQNSLYGNISRFSHGECSN-GSLNLSMSYMDLVGL-YSSFFYRNALINVGSGHSPFSLSEF--VVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISLNCESVKENPNLQPCH-TD*SSSELERHHFGNISQGGRSPRENI-QKIRSEFNQIEIASITSASVIFSVLIALIFFLVCMKKFACNAVSDHV-SGRKEVVTCNSISIQLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLGRFQGVQQFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQERSRRTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWASMLLAQGRGCEFFMARLWDSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRLKAIQPPT 3622
            L G+LSP +  L +LRVLSL FN   GE+P E+  +  LEVLDL                        GFN F G IP++L    SL V+NL+GN +NG++    G +     V  S+N L G IP E+GE+CG L HL L+ N L   IP +LGNC EL++++L SNIL++ IP+ LG+L  LE LD+SRN L G VP  LG+C +L +L+L N    LFS         G S +         ++N+FEG +P  I+ L  + +LW P  NLEG F  +WG C +L+ LN+AQN  TG  P   G CKNL+FLDLS+NNL+G L   +PVPCM VF++S N L G I +FS G+C++  S + ++   D   L Y SFF    L     G    SL E    V H+F +N F  S+    I+   L   L Y   +  N   G      F+ C  L+ L+ + S N + G++P   G  C+ LK L  + N+  G IP    DMVSL+ LNLS NRL G I   IG++K +++LSL++NN  G++P  L +L SLEVL+LSSNSL+GEI   +  L++L+ + L++NKL+GQIP G +N++ LS FNVSFNNL+GS P N  S+ C +   NP L+ C+    +    ++    N S    +P E   +K  + FN IEIASITSAS I SVL+ALI   +  +K+  N  S  V S RKEV     I + LT+ENVVRATG FN  NCIG+GGFGATYKAE+VPG +VA+KRL++GRFQGVQQF AEIKTLGR++HPNLVTLIGYH SE EMFLIYNYLPGGNLE FIQERS R V+WR+LHKIALDIA AL +LHD+CVPRVLHRD+KPSNILLD  +NAYLSDFGLARLLGTSETHATT VAGTFGYVAPEYAMTCRVSDKADVYSYGVV+L LLSDKKALDPSFSS+GNGFNIV WA MLL QG+  EFF A LWD+GP+ DL+EVLHLAV+CT +S S+RPSM+ V RRLK +QPP+
Sbjct:   97 LFGKLSPKLSELAELRVLSLPFNGLEGEIPEEIWGMEKLEVLDLEGNLISGVLPIRFNGLKNLRVLNLGFNRFVGEIPSSLSNVKSLEVLNLAGNGINGSVSGFVGRLRG---VYLSYNLLGGAIPEEIGEHCGQLEHLDLSGNLLMQGIPGSLGNCSELRTVLLHSNILEDVIPAELGRLRKLEVLDVSRNTLGGQVPMELGNCTELSVLLLSN----LFSSVPDVNGTLGDSGVEQMVAMNIDEFNYFEGPVPVEIMNLPKLRLLWAPRANLEGSFMSSWGKCDSLEMLNLAQNDFTGDFPNQLGGCKNLHFLDLSANNLTGVLAEELPVPCMTVFDVSGNVLSGPIPQFSVGKCASVPSWSGNLFETDDRALPYKSFFASKIL----GGPILASLGEVGRSVFHNFGQNNFV-SMESLPIARDKLGKGLVYAILVGENKLAGPFPTNLFEKCDGLNALLLNVSYNMLSGQIPSKFGRMCRSLKFLDASGNQITGPIPVGLGDMVSLVSLNLSRNRLQGQILVSIGQLKHLKFLSLADNNIGGSIPTSLGRLYSLEVLDLSSNSLTGEIPKGIENLRNLTDVLLNNNKLSGQIPAGLANVSTLSAFNVSFNNLSGSFPSNGNSIKCSNAVGNPFLRSCNEVSLAVPSADQGQVDNSSSYTAAPPEVTGKKGGNGFNSIEIASITSASAIVSVLLALIVLFIYTQKW--NPRSRVVGSMRKEVTVFTDIGVPLTFENVVRATGNFNASNCIGNGGFGATYKAEIVPGNLVAIKRLAVGRFQGVQQFHAEIKTLGRLRHPNLVTLIGYHASETEMFLIYNYLPGGNLEKFIQERSTRAVDWRILHKIALDIARALAYLHDQCVPRVLHRDVKPSNILLDDDYNAYLSDFGLARLLGTSETHATTGVAGTFGYVAPEYAMTCRVSDKADVYSYGVVLLELLSDKKALDPSFSSYGNGFNIVAWACMLLRQGQAKEFFAAGLWDAGPEDDLVEVLHLAVVCTVDSLSTRPSMKHVVRRLKQLQPPS 1106          

HSP 2 Score: 74.7146 bits (182), Expect = 4.329e-10
Identity = 46/136 (33.82%), Postives = 73/136 (53.68%), Query Frame = 2

Query:  566 KTAEVKGDQVRGNFTLLYPCLHVDVDDNISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPN 973
            K  +  G+Q+ G   +    +   V  N+S   L+G++  +IG L  L+ LSL  N   G +P  +G+L  LEVLDL  N+  G IP  ++   +L  + L+ N+L+G IPA    +++    + SFN LSG  P+
Sbjct:  570 KFLDASGNQITGPIPVGLGDMVSLVSLNLSRNRLQGQILVSIGQLKHLKFLSLADNNIGGSIPTSLGRLYSLEVLDLSSNSLTGEIPKGIENLRNLTDVLLNNNKLSGQIPAGLANVSTLSAFNVSFNNLSGSFPS 705          

BLAST of g.barbadense_cottongen_reftransV1_0097180 vs. ExPASy TrEMBL
Match: I1KWF2_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=2)

HSP 1 Score: 890.567 bits (2300), Expect = 0.000e+0
Identity = 541/1024 (52.83%), Postives = 680/1024 (66.41%), Query Frame = 2

Query:  665 LRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDL------------------------GFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPNELGENCGSLMHLHLASNELSGSIPSNLGNCGELKSLILSSNILQNDIPSSLGKLENLEALDLSRNFLSGLVPPTLGDCXXXXXXXXXXXXGPLFSRK-------GSSFMFHQEEYGEGDYNFFEGELPESIVKLYGIHVLWLPNVNLEGIFPQTWGSCSNLKRLNVAQNFLTGQIPASFGNCKNLYFXXXXXXXXXXXXPAAIPVPCMVVFNISQNSLYGNISRFSHGECSN-GSLNLSMSYMDLVGL-YSSFFYRNALINVGSGHSPFSLSEF--VVLHDFSRNQFTGSVPPFIISLYTLSAKLNYGFWLNGNNFEGNLSV*SFDPCLRLDGLIFDASNNKIVGELPLNMGHTCKCLKILSLASNEFVGSIPTSFTDMVSLLKLNLSENRL*GPIPSYIGEMKEIRYLSLSNNNFSGAMPWDXXXXXXXXXXXXXXXXXXGEILPDLAELKHLSALRLDHNKLTGQIPFGFSNMTALSVFNVSFNNLTGSIPLNSISLNCESVKENPNLQPCH-TD*SSSELERHHFGNISQGGRSPRENI-QKIRSEFNQIEIASITSASVIFSVLIALIFFLVCMKKFACNAVSDHV-SGRKEVVTCNSISIQLTYENVVRATGCFNLQNCIGSGGFGATYKAEXXXXXXXXXKRLSLGRFQGVQQFAAEIKTLGRVQHPNLVTLIGYHVSEAEMFLIYNYLPGGNLENFIQERSRRTVEWRMLHKIALDIACALMHLHDECVPRVLHRDIKPSNILLDKHFNAYLSDFGLARLLGTSETHATTDVAGTFGYVAPEYAMTCRVSDKADVYSYGXXXXXXXSDKKALDPSFSSFGNGFNIVTWASMLLAQGRGCEFFMARLWDSGPQHDLIEVLHLAVMCTGESPSSRPSMREVARRLKAIQPPT 3622
            L G+LSP +  L +LRVLSL FN   GE+P E+  +  LEVLDL                        GFN F G IP++L    SL V+NL+GN +NG++    G +     V  S+N L G IP E+GE+CG L HL L+ N L   IP +LGNC EL++++L SNIL++ IP+ LG+L  LE LD+SRN L G VP  LG+C +L +L+L N    LFS         G S +         ++N+FEG +P  I+ L  + +LW P  NLEG F  +WG C +L+ LN+AQN  TG  P   G CKNL+FLDLS+NNL+G L   +PVPCM VF++S N L G I +FS G+C++  S + ++   D   L Y SFF    L     G    SL E    V H+F +N F  S+    I+   L   L Y   +  N   G      F+ C  L+ L+ + S N + G++P   G  C+ LK L  + N+  G IP    DMVSL+ LNLS NRL G I   IG++K +++LSL++NN  G++P  L +L SLEVL+LSSNSL+GEI   +  L++L+ + L++NKL+GQIP G +N++ LS FNVSFNNL+GS P N  S+ C +   NP L+ C+    +    ++    N S    +P E   +K  + FN IEIASITSAS I SVL+ALI   +  +K+  N  S  V S RKEV     I + LT+ENVVRATG FN  NCIG+GGFGATYKAE+VPG +VA+KRL++GRFQGVQQF AEIKTLGR++HPNLVTLIGYH SE EMFLIYNYLPGGNLE FIQERS R V+WR+LHKIALDIA AL +LHD+CVPRVLHRD+KPSNILLD  +NAYLSDFGLARLLGTSETHATT VAGTFGYVAPEYAMTCRVSDKADVYSYGVV+L LLSDKKALDPSFSS+GNGFNIV WA MLL QG+  EFF A LWD+GP+ DL+EVLHLAV+CT +S S+RPSM+ V RRLK +QPP+
Sbjct:  126 LFGKLSPKLSELAELRVLSLPFNGLEGEIPEEIWGMEKLEVLDLEGNLISGVLPIRFNGLKNLRVLNLGFNRFVGEIPSSLSNVKSLEVLNLAGNGINGSVSGFVGRLRG---VYLSYNLLGGAIPEEIGEHCGQLEHLDLSGNLLMQGIPGSLGNCSELRTVLLHSNILEDVIPAELGRLRKLEVLDVSRNTLGGQVPMELGNCTELSVLLLSN----LFSSVPDVNGTLGDSGVEQMVAMNIDEFNYFEGPVPVEIMNLPKLRLLWAPRANLEGSFMSSWGKCDSLEMLNLAQNDFTGDFPNQLGGCKNLHFLDLSANNLTGVLAEELPVPCMTVFDVSGNVLSGPIPQFSVGKCASVPSWSGNLFETDDRALPYKSFFASKIL----GGPILASLGEVGRSVFHNFGQNNFV-SMESLPIARDKLGKGLVYAILVGENKLAGPFPTNLFEKCDGLNALLLNVSYNMLSGQIPSKFGRMCRSLKFLDASGNQITGPIPVGLGDMVSLVSLNLSRNRLQGQILVSIGQLKHLKFLSLADNNIGGSIPTSLGRLYSLEVLDLSSNSLTGEIPKGIENLRNLTDVLLNNNKLSGQIPAGLANVSTLSAFNVSFNNLSGSFPSNGNSIKCSNAVGNPFLRSCNEVSLAVPSADQGQVDNSSSYTAAPPEVTGKKGGNGFNSIEIASITSASAIVSVLLALIVLFIYTQKW--NPRSRVVGSMRKEVTVFTDIGVPLTFENVVRATGNFNASNCIGNGGFGATYKAEIVPGNLVAIKRLAVGRFQGVQQFHAEIKTLGRLRHPNLVTLIGYHASETEMFLIYNYLPGGNLEKFIQERSTRAVDWRILHKIALDIARALAYLHDQCVPRVLHRDVKPSNILLDDDYNAYLSDFGLARLLGTSETHATTGVAGTFGYVAPEYAMTCRVSDKADVYSYGVVLLELLSDKKALDPSFSSYGNGFNIVAWACMLLRQGQAKEFFAAGLWDAGPEDDLVEVLHLAVVCTVDSLSTRPSMKHVVRRLKQLQPPS 1135          

HSP 2 Score: 74.7146 bits (182), Expect = 4.298e-10
Identity = 46/136 (33.82%), Postives = 73/136 (53.68%), Query Frame = 2

Query:  566 KTAEVKGDQVRGNFTLLYPCLHVDVDDNISFVGLRGRLSPAIGHLTKLRVLSLGFNEFFGELPLEMGKLNLLEVLDLGFNAFHGPIPAALKKCTSLRVINLSGNQLNGTIPAIFGPITSFQVVDFSFNKLSGEIPN 973
            K  +  G+Q+ G   +    +   V  N+S   L+G++  +IG L  L+ LSL  N   G +P  +G+L  LEVLDL  N+  G IP  ++   +L  + L+ N+L+G IPA    +++    + SFN LSG  P+
Sbjct:  599 KFLDASGNQITGPIPVGLGDMVSLVSLNLSRNRLQGQILVSIGQLKHLKFLSLADNNIGGSIPTSLGRLYSLEVLDLSSNSLTGEIPKGIENLRNLTDVLLNNNKLSGQIPAGLANVSTLSAFNVSFNNLSGSFPS 734