g.barbadense_cottongen_reftransV1_0097138, g.barbadense_cottongen_reftransV1_0097138 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PP168_ARATH (Pentatricopeptide repeat-containing protein At2g22070 OS=Arabidopsis thaliana GN=PCMP-H41 PE=3 SV=1)

HSP 1 Score: 430.639 bits (1106), Expect = 2.289e-135
Identity = 223/591 (37.73%), Postives = 348/591 (58.88%), Query Frame = -3

Query:  591 ALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRV-RPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERN--IGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETK-EKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2351
            A++ F     + +  +N++I G  +  +   ++  F  MLR  +  PD+ T   VL + A L    +G  +H  I+ +G +    V  +L+ MY                                    + G +E A  L E    ++  I  + + ++G +K GD+N+A  +F  +K++DVV+WT ++ G  Q+G + +A+++F  M+  G RPN  TL + LS  + + +L  G +IH   V++G   + + + AL+ MYAK GNI SAS+ F+  + E+D  +W+ MI   A HG   +A+  F+ M+  G++PD + ++ + +AC+H+G V+ G  +FD M+    I PT+ HY  +VDL GRAG L EA +FI++MPI PD V WG+L  ACR HKNI + ++ +E+LL LEP++ G+Y  L+N+Y+A G+WE+  ++R  M++  V K+ G+S+IEV  +VH F   D  H    EIY+ +++I    ++ GY+P+T  VLH++EEE KE  L  HSEKLA+AF L++T   TT+RI+KNLRVC DCH+ +K  SK+  REII+RD  RFHHFK G CSC DYW
Sbjct:  231 AMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMY-----------------------------------SRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFSACTHAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLLSACRVHKNIDLGKVAAERLLLLEPENSGAYSALANLYSACGKWEEAAKIRKSMKDGRVKKEQGFSWIEVKHKVHVFGVEDGTHPEKNEIYMTMKKIWDEIKKMGYVPDTASVLHDLEEEVKEQILRHHSEKLAIAFGLISTPDKTTLRIMKNLRVCNDCHTAIKFISKLVGREIIVRDTTRFHHFKDGFCSCRDYW 786          

HSP 2 Score: 138.658 bits (348), Expect = 2.708e-32
Identity = 89/312 (28.53%), Postives = 148/312 (47.44%), Query Frame = -3

Query: 1347 KSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKV-------------------------------FEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMF-SGIKPDAVVFLALLTACSHSGQVDLG 2186
            KSV     RF   ++H R+IKSG+ F                                   S+ L N L+N   K G    A +LF+ MP R   SWN+ ++   K GD++   + FD++ ++D VSWTT++ G    G + KA+ +   M++ G+ P   TL + L++ A    +E G ++H++ V+ GLR N + + +L++MYAKCG+ + A  V                               FE+  E+DI TW+ MI G+   G+  +A+  F KM+  S + PD     ++L+AC++  ++ +G
Sbjct:   22 KSVNKSNGRFTAQLVHCRVIKSGLMF-----------------------------------SVYLMNNLMNVYSKTGYALHARKLFDEMPLRTAFSWNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIVGYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIG 298          

HSP 3 Score: 137.887 bits (346), Expect = 4.817e-32
Identity = 115/449 (25.61%), Postives = 186/449 (41.43%), Query Frame = -3

Query: 1101 FNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVG-LRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEET---------------------------------KEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLR--LEPKH 2339
            F+    +    +  +I G      +  ++     M+++ + P + T   VL SVA       G  +H  I+K G+  +  V  SL+ MY K  +   A  VFD    R+ S     WN +I    +VG ++ AM  FE M ER+I +WNS I+G                                +Q G   +AL +F KML    L P+  TL S LSACA +  L  G +IH++ V  G  ++     AL+ MY++CG + +A ++ E+                                  K++D+  W+ MI G+  HG YG+AI  F+ M+  G +P++    A+L+  S    +  G     S      I        L+  +  +AG +  A +    +    D V+W ++  A   H + + A  + E +L   L P H
Sbjct:  103 FDQLPQRDSVSWTTMIVGYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISS----WNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGF-------------------------------NQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALIT-MYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDH 515          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PPR25_ARATH (Pentatricopeptide repeat-containing protein At1g09410 OS=Arabidopsis thaliana GN=PCMP-H18 PE=2 SV=2)

HSP 1 Score: 426.402 bits (1095), Expect = 1.160e-134
Identity = 213/529 (40.26%), Postives = 326/529 (61.63%), Query Frame = -3

Query:  591 GLILHGRIIKSGVEFDSFVR------VSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSG-IKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLK-LEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2153
            GL   GR+ ++   FD           ++V  Y +   +  A ++FD  PE+ +    + W  ++ G  + G +E A ELFE MP + + + N+ I+GL + G++ KA ++FD MKE++  SW T++    +NG   +AL +F  M + G+RP   TL+S LS CA + +L  G ++H   V     ++   A+ L+ MY KCG ++ +  +F+    KDI  W+ +I G+A HG   +A++ F +M  SG  KP+ V F+A L+ACS++G V+ GL  ++SM   + ++P   HY  +VD+LGRAGR +EAM+ I  M + PD   WG+L  ACR H  + +AE  ++KL+ +EP++ G+Y+ LSN+YA+ GRW DV  +R LM+ + V K PG S+ EV  +VH+F  G  +    +E  LK L+E+    RE GY P+  + LH+++EEEK ++L  HSE+LA+A+AL+  S G  IR++KNLRVC DCH+ +K  SK+ +REIILRD  RFHHF+ G CSC DYW
Sbjct:  181 GLCKEGRVDEAREIFDEMSERSVITWTTMVTGYGQNNRVDDARKIFDVMPEKTE----VSWTSMLMGYVQNGRIEDAEELFEVMPVKPVIACNAMISGLGQKGEIAKARRVFDSMKERNDASWQTVIKIHERNGFELEALDLFILMQKQGVRPTFPTLISILSVCASLASLHHGKQVHAQLVRCQFDVDVYVASVLMTMYIKCGELVKSKLIFDRFPSKDIIMWNSIISGYASHGLGEEALKVFCEMPLSGSTKPNEVTFVATLSACSYAGMVEEGLKIYESMESVFGVKPITAHYACMVDMLGRAGRFNEAMEMIDSMTVEPDAAVWGSLLGACRTHSQLDVAEFCAKKLIEIEPENSGTYILLSNMYASQGRWADVAELRKLMKTRLVRKSPGCSWTEVENKVHAFTRGGINSHPEQESILKILDELDGLLREAGYNPDCSYALHDVDEEEKVNSLKYHSERLAVAYALLKLSEGIPIRVMKNLRVCSDCHTAIKIISKVKEREIILRDANRFHHFRNGECSCKDYW 705          

HSP 2 Score: 108.227 bits (269), Expect = 7.677e-23
Identity = 88/361 (24.38%), Postives = 161/361 (44.60%), Query Frame = -3

Query: 1110 VRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKM----------MFSG------------------IKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMK---FIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLE 2099
            VR++ +    K+ E   A ++FD       S+SI  WN ++ G         A +LF+ MP+RNI SWN  ++G MKNG++++A ++FD M E++VVSWT +V G   NG    A S+F+KM E     N ++    L    + G ++   +++    +     +     +++    K G +  A ++F+E  E+ + TW+ M+ G+  +     A + F  M          M  G                  +KP  +   A+++     G++      FDSM+     E     +  V+ +  R G   EA+     +Q+  + P F    ++   C +  ++   + V  +L+R +
Sbjct:   22 VRITHLSRIGKIHE---ARKLFDSC----DSKSISSWNSMVAGYFANLMPRDARKLFDEMPDRNIISWNGLVSGYMKNGEIDEARKVFDLMPERNVVSWTALVKGYVHNGKVDVAESLFWKMPE----KNKVSWTVMLIGFLQDGRIDDACKLYEMIPDK----DNIARTSMIHGLCKEGRVDEAREIFDEMSERSVITWTTMVTGYGQNNRVDDARKIFDVMPEKTEVSWTSMLMGYVQNGRIEDAEELFEVMPVKP-VIACNAMISGLGQKGEIAKARRVFDSMK-----ERNDASWQTVIKIHERNGFELEALDLFILMQKQGVRPTFPTLISILSVCASLASLHHGKQVHAQLVRCQ 361          

HSP 3 Score: 90.1225 bits (222), Expect = 3.876e-17
Identity = 75/337 (22.26%), Postives = 142/337 (42.14%), Query Frame = -3

Query:  978 NVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYI 1988
            NV I    ++G + +A +LF++   ++I SWNS + G   N     A +LFDEM +++++SW  +V+G  +NG+  +A  +F  M E                                        N  +  ALV  Y   G +  A  +F +  EK+  +W+VM+ G+   G    A + ++ +       D +   +++      G+VD     FD M      E ++  +T +V   G+  R+D+A K    MP   + V+W ++      +  I+ A    E+L  + P  P   +  + + + +G+  ++ + R +  +     D  W  +
Sbjct:   21 NVRITHLSRIGKIHEARKLFDSCDSKSISSWNSMVAGYFANLMPRDARKLFDEMPDRNIISWNGLVSGYMKNGEIDEARKVFDLMPE---------------------------------------RNVVSWTALVKGYVHNGKVDVAESLFWKMPEKNKVSWTVMLIGFLQDGRIDDACKLYEMIP----DKDNIARTSMIHGLCKEGRVDEAREIFDEMS-----ERSVITWTTMVTGYGQNNRVDDARKIFDVMPEKTE-VSWTSMLMGYVQNGRIEDA----EELFEVMPVKP--VIACNAMISGLGQKGEIAKARRVFDSMKERNDASWQTV 302          

HSP 4 Score: 65.855 bits (159), Expect = 1.438e-9
Identity = 64/292 (21.92%), Postives = 131/292 (44.86%), Query Frame = -3

Query: 1506 ALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMP-----ERNIGSWNSFINGLMKNGDLNKAMQLFDEMKE----KDVVS-WTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIR 2351
            A  +F+    ++   +  +I+    N     ++  F+LM +Q VRP   T   +L   A L     G  +H ++++   + D +V   L+ MY+K  E+  +  +FD  P    S+ I++WN +I+G    G  E+A+++F  MP     + N  ++ + ++     G + + +++++ M+     K + + +  +V+ + + G   +A+ M   M    + P+     S L AC     L+         +E     N  T   L +MYA  G     +++ +  K + +R
Sbjct:  284 ARRVFDSMKERNDASWQTVIKIHERNGFELEALDLFILMQKQGVRPTFPTLISILSVCASLASLHHGKQVHAQLVRCQFDVDVYVASVLMTMYIKCGELVKSKLIFDRFP----SKDIIMWNSIISGYASHGLGEEALKVFCEMPLSGSTKPNEVTFVATLSACSYAGMVEEGLKIYESMESVFGVKPITAHYACMVDMLGRAGRFNEAMEMIDSMT---VEPDAAVWGSLLGACRTHSQLDVAEFCAKKLIEIEPE-NSGTYILLSNMYASQGRWADVAELRKLMKTRLVR 567          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PP251_ARATH (Putative pentatricopeptide repeat-containing protein At3g23330 OS=Arabidopsis thaliana GN=PCMP-H32 PE=3 SV=1)

HSP 1 Score: 422.55 bits (1085), Expect = 4.171e-133
Identity = 240/697 (34.43%), Postives = 383/697 (54.95%), Query Frame = -3

Query:  591 QLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGL-GLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFAL---QVFDESPERN--------KSESILL---------------------WNVLINGCCKVGNLEKAMELFEAMPERN-----------------------------------------IGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2459
            Q +Q+HAQ +R   S S    +++IS+ ++LK +  AL +F       +  + ++IR  T+ S F  +++ F+ M      PD   FP VLKS   +  LRF G  +HG I++ G++ D +   +L+ MY KL  MG  +    VFDE P+R         K+E+ ++                     +N +I G  + G  E A+ +   M   +                                         IGS  S ++   K+  +  + ++F  +  +D +SW ++V G  QNG + +AL +F +M+   ++P  +   S + ACA +  L  G ++H Y +  G   N   A+ALVDMY+KCGNI +A K+F+     D  +W+ +I G A+HG   +A+  F++M   G+KP+ V F+A+LTACSH G VD    +F+SM   Y +   ++HY  V DLLGRAG+L+EA  FI +M + P    W  L  +C  HKN+++AE V+EK+  ++ ++ G+YV + N+YA+ GRW+++ ++R+ M+ + + K P  S+IE+  + H FV+GD  H    +I   L+ ++    ++GY+ +T  VLH+++EE K + L  HSE+LA+AF ++NT PGTTIR+ KN+R+C DCH  +K  SK+++REII+RD  RFHHF  G CSCGDYW
Sbjct:   23 QAKQLHAQFIRTQ-SLSHTSASIVISIYTNLKLLHEALLLFKTLKSPPVLAWKSVIRCFTDQSLFSKALASFVEMRASGRCPDHNVFPSVLKSCTMMMDLRF-GESVHGFIVRLGMDCDLYTGNALMNMYAKLLGMGSKISVGNVFDEMPQRTSNSGDEDVKAETCIMPFGIDSVRRVFEVMPRKDVVSYNTIIAGYAQSGMYEDALRMVREMGTTDLKPDSFTLSSVLPIFSEYVDVIKGKEIHGYVIRKGIDSDVYIGS--SLVDMYAKSARIEDSERVFSRLYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVLRGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNVLDEVSWTAIIMGHALHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVGLVDEAWGYFNSMTKVYGLNQELEHYAAVADLLGRAGKLEEAYNFISKMCVEPTGSVWSTLLSSCSVHKNLELAEKVAEKIFTVDSENMGAYVLMCNMYASNGRWKEMAKLRLRMRKKGLRKKPACSWIEMKNKTHGFVSGDRSHPSMDKINEFLKAVMEQMEKEGYVADTSGVLHDVDEEHKRELLFGHSERLAVAFGIINTEPGTTIRVTKNIRICTDCHVAIKFISKITEREIIVRDNSRFHHFNRGNCSCGDYW 715          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PP341_ARATH (Pentatricopeptide repeat-containing protein At4g30700 OS=Arabidopsis thaliana GN=DYW9 PE=2 SV=1)

HSP 1 Score: 423.705 bits (1088), Expect = 1.418e-132
Identity = 230/624 (36.86%), Postives = 341/624 (54.65%), Query Frame = -3

Query:  591 ALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQR-VRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAM----------------P----------------ERNIGSWNSFINGLM----KNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2351
            A  +F+    K   L+N +I G  +N  +  S+  F  ++ +   R D  T   +L +VA L    LG+ +H    K+G     +V    + +Y K  ++     +F E     +   I+ +N +I+G    G  E ++ LF+ +                P                + N  S  S    L     K  ++  A +LFDE  EK + SW  +++G +QNG  + A+S+F +M +    PN +T+   LSACA++GAL  G  +H+         +   + AL+ MYAKCG+I  A ++F+   +K+  TW+ MI G+ +HG   +A+  F +M+ SGI P  V FL +L ACSH+G V  G   F+SM   Y  EP++KHY  +VD+LGRAG L  A++FI+ M I P    W  L  ACR HK+  +A  VSEKL  L+P + G +V LSN+++A   +     VR   + + + K PG++ IE+    H F +GD  H + +EIY KLE++    RE GY PET   LH++EEEE+E  +  HSE+LA+AF L+ T PGT IRI+KNLRVC DCH++ K  SK+++R I++RD  RFHHFK GVCSCGDYW
Sbjct:  173 ARKVFDRMPEKDTILWNTMISGYRKNEMYVESIQVFRDLINESCTRLDTTTLLDILPAVAELQELRLGMQIHSLATKTGCYSHDYVLTGFISLYSKCGKIKMGSALFREF----RKPDIVAYNAMIHGYTSNGETELSLSLFKELMLSGARLRSSTLVSLVPVSGHLMLIYAIHGYCLKSNFLSHASVSTALTTVYSKLNEIESARKLFDESPEKSLPSWNAMISGYTQNGLTEDAISLFREMQKSEFSPNPVTITCILSACAQLGALSLGKWVHDLVRSTDFESSIYVSTALIGMYAKCGSIAEARRLFDLMTKKNEVTWNTMISGYGLHGQGQEALNIFYEMLNSGITPTPVTFLCVLYACSHAGLVKEGDEIFNSMIHRYGFEPSVKHYACMVDILGRAGHLQRALQFIEAMSIEPGSSVWETLLGACRIHKDTNLARTVSEKLFELDPDNVGYHVLLSNIHSADRNYPQAATVRQTAKKRKLAKAPGYTLIEIGETPHVFTSGDQSHPQVKEIYEKLEKLEGKMREAGYQPETELALHDVEEEERELMVKVHSERLAIAFGLIATEPGTEIRIIKNLRVCLDCHTVTKLISKITERVIVVRDANRFHHFKDGVCSCGDYW 792          

HSP 2 Score: 158.303 bits (399), Expect = 1.548e-38
Identity = 131/517 (25.34%), Postives = 234/517 (45.26%), Query Frame = -3

Query: 1065 KTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQR-VRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNK----------------SESILLWNVLINGCCKVGNLEKAMELFEAMPE--------------RNIGSWN------SFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYA 2504
            K  +    K + +   L Q HAQI+     +  +L T L    S L +I YA  IF       +FLFN L+RG + N    SS+S F  + +   ++P+  T+ F + + +G      G ++HG+ +  G + +  +  ++V+MY K   +  A +VFD  PE++                  ESI ++  LIN  C   +    +++  A+ E                 G ++       FI+   K G +     LF E ++ D+V++  +++G + NG+ + +LS+F +++  G R    TLVS +     +  + A   IH Y +++    + + + AL  +Y+K   I SA K+F+E+ EK + +W+ MI G+  +G    AI  F++M  S   P+ V    +L+AC+  G + LG    D +R     E ++   T ++ +  + G + EA +    M    + V W  +      H   + A  +  ++L      P    FL  +YA
Sbjct:   20 KNTYLDFFKRSTSISHLAQTHAQIILHGFRNDISLLTKLTQRLSDLGAIYYARDIFLSVQRPDVFLFNVLMRGFSVNESPHSSLSVFAHLRKSTDLKPNSSTYAFAISAASGFRDDRAGRVIHGQAVVDGCDSELLLGSNIVKMYFKFWRVEDARKVFDRMPEKDTILWNTMISGYRKNEMYVESIQVFRDLINESCTRLDTTTLLDILPAVAELQELRLGMQIHSLATKTGCYSHDYVLTGFISLYSKCGKIKMGSALFREFRKPDIVAYNAMIHGYTSNGETELSLSLFKELMLSGARLRSSTLVSLVPVSGHLMLIYA---IHGYCLKSNFLSHASVSTALTTVYSKLNEIESARKLFDESPEKSLPSWNAMISGYTQNGLTEDAISLFREMQKSEFSPNPVTITCILSACAQLGALSLGKWVHDLVR-STDFESSIYVSTALIGMYAKCGSIAEARRLFDLM-TKKNEVTWNTMISGYGLHGQGQEALNIFYEMLN-SGITPTPVTFLCVLYA 530          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PPR57_ARATH (Pentatricopeptide repeat-containing protein At1g25360 OS=Arabidopsis thaliana GN=PCMP-H74 PE=2 SV=1)

HSP 1 Score: 423.32 bits (1087), Expect = 1.543e-132
Identity = 211/576 (36.63%), Postives = 329/576 (57.12%), Query Frame = -3

Query:  591 LFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEE-EKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2315
            L  +NA+I G      +Q ++     M+   +  D+ T+P V+++ A  GL  LG  +H  +++   +F      SLV +Y K                                    G  ++A  +FE MP +++ SWN+ ++G + +G + +A  +F EMKEK+++SW  +++G+++NG  ++ L +F  M   G  P D     A+ +CA +GA   G + H   ++ G   + +   AL+ MYAKCG +  A +VF      D  +W+ +I     HG   +A+  +++M+  GI+PD +  L +LTACSH+G VD G  +FDSM   Y I P   HY  ++DLL R+G+  +A   I+ +P  P    W AL   CR H N+++  + ++KL  L P+H G+Y+ LSN++AA G+WE+V RVR LM+++ V K+   S+IE+  QVH+F+  D  H  A  +Y+ L+++    R  GY+P+T +VLH++E +  KED L +HSEK+A+AF LM   PGTTIRI KNLR CGDCH+  +  S + QR+IILRD KRFHHF+ G CSCG++W
Sbjct:  251 LVAYNAMISGYVNRGFYQEALEMVRRMVSSGIELDEFTYPSVIRACATAGLLQLGKQVHAYVLRRE-DFSFHFDNSLVSLYYK-----------------------------------CGKFDEARAIFEKMPAKDLVSWNALLSGYVSSGHIGEAKLIFKEMKEKNILSWMIMISGLAENGFGEEGLKLFSCMKREGFEPCDYAFSGAIKSCAVLGAYCNGQQYHAQLLKIGFDSSLSAGNALITMYAKCGVVEEARQVFRTMPCLDSVSWNALIAALGQHGHGAEAVDVYEEMLKKGIRPDRITLLTVLTACSHAGLVDQGRKYFDSMETVYRIPPGADHYARLIDLLCRSGKFSDAESVIESLPFKPTAEIWEALLSGCRVHGNMELGIIAADKLFGLIPEHDGTYMLLSNMHAATGQWEEVARVRKLMRDRGVKKEVACSWIEMETQVHTFLVDDTSHPEAEAVYIYLQDLGKEMRRLGYVPDTSFVLHDVESDGHKEDMLTTHSEKIAVAFGLMKLPPGTTIRIFKNLRTCGDCHNFFRFLSWVVQRDIILRDRKRFHHFRNGECSCGNFW 790          

HSP 2 Score: 123.635 bits (309), Expect = 1.486e-27
Identity = 110/440 (25.00%), Postives = 190/440 (43.18%), Query Frame = -3

Query: 1116 KSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGL----RFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEE----MGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFV---------ENGL--------RLNKATAA-------------ALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLR 2321
            +   ++NA+I G + N+   S+++ F  M  +  +PD  TF  VL   AGL L        +  H   +KSG  + + V  +LV +Y K       +  A +VFDE  E+++      W  ++ G  K G  +   EL E M                            D MK   +V++  +++G    G +Q+AL M  +M+  G+  ++ T  S + ACA  G L+ G ++H Y +         +N L        + ++A A              AL+  Y   G+I  A  +F+E KEK+I +W +MI G A +GF  + ++ F  M   G +P    F   + +C+  G    G  +   +      + ++     ++ +  + G ++EA +  + MP   D V+W AL  A   H +   A  V E++L+
Sbjct:  111 RDTVMYNAMITGFSHNNDGYSAINLFCKMKHEGFKPDNFTFASVL---AGLALVADDEKQCVQFHAAALKSGAGYITSVSNALVSVYSKCASSPSLLHSARKVFDEILEKDERS----WTTMMTGYVKNGYFDLGEELLEGMD---------------------------DNMK---LVAYNAMISGYVNRGFYQEALEMVRRMVSSGIELDEFTYPSVIRACATAGLLQLGKQVHAYVLRREDFSFHFDNSLVSLYYKCGKFDEARAIFEKMPAKDLVSWNALLSGYVSSGHIGEAKLIFKEMKEKNILSWMIMISGLAENGFGEEGLKLFSCMKREGFEPCDYAFSGAIKSCAVLGAYCNGQQYHAQL-LKIGFDSSLSAGNALITMYAKCGVVEEARQVFRTMP-CLDSVSWNALIAALGQHGHGAEAVDVYEEMLK 511          

HSP 3 Score: 120.168 bits (300), Expect = 2.055e-26
Identity = 99/387 (25.58%), Postives = 174/387 (44.96%), Query Frame = -3

Query: 1122 LGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMP--ERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAG-VRIHNYFVENGLRLNKATAAALVDMYAKCGN----ILSASKVFEETKEKDIRTWSV--------------------------------MIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLG--LNFFDSMRFDYSIEPTMKHY-TLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKL 2156
            L   +HG II  G +  + +   L+++Y K  E+ +A Q+FDE  E +K    +    +++G C  G++  A  +FE  P   R+   +N+ I G   N D   A+ LF +MK +                               G +P++ T  S L+  A +   E   V+ H   +++G     + + ALV +Y+KC +    + SA KVF+E  EKD R+W+                                 MI G+   GFY +A+   ++M+ SGI+ D   + +++ AC+ +G + LG  ++ +   R D+S      H+   +V L  + G+ DEA    ++MP + D V+W AL     +  +I  A+L+ +++
Sbjct:   32 LARAVHGNIITFGFQPRAHILNRLIDVYCKSSELNYARQLFDEISEPDK----IARTTMVSGYCASGDITLARGVFEKAPVCMRDTVMYNAMITGFSHNNDGYSAINLFCKMKHE-------------------------------GFKPDNFTFASVLAGLALVADDEKQCVQFHAAALKSGAGYITSVSNALVSVYSKCASSPSLLHSARKVFDEILEKDERSWTTMMTGYVKNGYFDLGEELLEGMDDNMKLVAYNAMISGYVNRGFYQEALEMVRRMVSSGIELDEFTYPSVIRACATAGLLQLGKQVHAYVLRREDFSF-----HFDNSLVSLYYKCGKFDEARAIFEKMP-AKDLVSWNALLSGYVSSGHIGEAKLIFKEM 377          

HSP 4 Score: 73.9442 bits (180), Expect = 4.299e-12
Identity = 72/297 (24.24%), Postives = 116/297 (39.06%), Query Frame = -3

Query: 1467 IFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYF--VENGLRLNKATA--AALVDMYAKCGNILSASKVFEETKEKDI-RTWSVMIWGWAIHG 2342
            IF     K++  +  +I GL EN   +  +  F  M R+   P    F   +KS A LG    G   H +++K G  FDS                                 S+   N LI    K G +E+A ++F  MP  +  SWN+ I  L ++G   +A+ +++EM +K                               G+RP+ +TL++ L+AC+  G ++ G +   YF  +E   R+       A L+D+  + G    A  V E    K     W  ++ G  +HG
Sbjct:  373 IFKEMKEKNILSWMIMISGLAENGFGEEGLKLFSCMKREGFEPCDYAFSGAIKSCAVLGAYCNGQQYHAQLLKIG--FDS---------------------------------SLSAGNALITMYAKCGVVEEARQVFRTMPCLDSVSWNALIAALGQHGHGAEAVDVYEEMLKK-------------------------------GIRPDRITLLTVLTACSHAGLVDQGRK---YFDSMETVYRIPPGADHYARLIDLLCRSGKFSDAESVIESLPFKPTAEIWEALLSGCRVHG 600          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: A0A0D2PM74_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G031300 PE=4 SV=1)

HSP 1 Score: 1332.01 bits (3446), Expect = 0.000e+0
Identity = 691/702 (98.43%), Postives = 697/702 (99.29%), Query Frame = -3

Query:  591 MSKFPCTKPTLTITVQMKSLRLLFERNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSQDPLKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2696
            MSKFP TKP LTITVQMKSLRLLFERNSSPAKTTSSSSKFKRPKPSSISNGSDSSQSSSSQDPLKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANLTTLLISVSSSLKSIPYALSIFN+SHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLR RVRPDKLT+PFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCC+VG+LEKA ELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNG+SQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWA HGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLL+LEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW
Sbjct:    1 MSKFPSTKPILTITVQMKSLRLLFERNSSPAKTTSSSSKFKRPKPSSISNGSDSSQSSSSQDPLKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANLTTLLISVSSSLKSIPYALSIFNNSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRHRVRPDKLTYPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCRVGDLEKATELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGLSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWATHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLQLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 702          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: A0A061EK73_THECC (Tetratricopeptide repeat-like superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_020290 PE=4 SV=1)

HSP 1 Score: 1147.88 bits (2968), Expect = 0.000e+0
Identity = 585/686 (85.28%), Postives = 630/686 (91.84%), Query Frame = -3

Query:  591 MKSLRLLFERNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSQDPLKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2648
            MKSLRLLFERNSSPAKT SSSS FK+PKP      S SS     QDPLKTHF+SLI+S++TTLQLRQIHAQI RR+LSSS+NLTTLLIS SSSLKSIPYA+S+FNH HHKS+FLFNALIRGLT+NS  +SS+SHFLLML   VRPDKLT+PFVLKS+AGLGLR LGLILHGRIIKSGVEFDSFVRV+LVEMYVKL+E+GFALQVFDESPERNKS SILLWNVLING CK GNL KAMELFEA PERNIGSWNS ING M+NGDL+KA++LFDEMKEKDVVSWTT+VNG SQNGDH+KALSMFFKMLE  LRPNDLTLV ALSACAKIGALEAG RIH+Y +ENG RLNKA  AALVDMYAKCG+I SASKVF+ETKE+DI TWSVMIWGWAIHG+Y QAI+CFKKMMFSGIKPD VVFLALLTACSHSGQV+LGLNFFDSMR DYSIEPTMKHYTLVVDLLGRAG+LDE++KFIQRMP+SPDFV WGALFCACRAHKNIKMAELVS+ LL+LEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQN+AV KDPGWSYIEV G++HSFVAGDH HK AREIYLKLEEIV+G R+ GYMPETGWVLHNIEEEEKEDALGSHSEKLALAFAL+ TSPGTTIRIVKNLRVCGDCHSLMK ASKMSQREI+LRDIKRFHHFK G CSCGDYW
Sbjct:    1 MKSLRLLFERNSSPAKTNSSSSSFKKPKPPISHGSSSSSS----QDPLKTHFASLIQSSKTTLQLRQIHAQIFRRNLSSSSNLTTLLISASSSLKSIPYAISLFNHFHHKSIFLFNALIRGLTDNSLLESSISHFLLMLSLGVRPDKLTYPFVLKSIAGLGLRCLGLILHGRIIKSGVEFDSFVRVALVEMYVKLKELGFALQVFDESPERNKSGSILLWNVLINGYCKDGNLGKAMELFEATPERNIGSWNSLINGFMRNGDLDKAVELFDEMKEKDVVSWTTMVNGFSQNGDHEKALSMFFKMLEAALRPNDLTLVPALSACAKIGALEAGARIHDYVLENGFRLNKAIGAALVDMYAKCGDIQSASKVFDETKERDILTWSVMIWGWAIHGYYEQAIQCFKKMMFSGIKPDGVVFLALLTACSHSGQVNLGLNFFDSMRLDYSIEPTMKHYTLVVDLLGRAGQLDESLKFIQRMPMSPDFVTWGALFCACRAHKNIKMAELVSQNLLQLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNRAVDKDPGWSYIEVGGEMHSFVAGDHAHKHAREIYLKLEEIVAGTRQHGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALIRTSPGTTIRIVKNLRVCGDCHSLMKYASKMSQREIVLRDIKRFHHFKDGACSCGDYW 682          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: F6GWJ6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0029g01130 PE=4 SV=1)

HSP 1 Score: 980.319 bits (2533), Expect = 0.000e+0
Identity = 474/638 (74.29%), Postives = 543/638 (85.11%), Query Frame = -3

Query:  591 KTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2504
            +THF  LI ++ T  QL QIHAQI   +L S++ + T LIS S SLKS+ YALSIF    H +LF+FNALIRGL ENS F+ SVSHF+LMLR  +RPD+LT PFVLKSVA L    LG  LHG ++K G+EFDSFVRVSLV+MYVK+ E+GF LQ+FDESP+RNK+ESILLWNVLINGCCKVG+L KA  LFEAMPERN GSWNS ING ++NGDL++A +LF +M EK+VVSWTT++NG SQNGDH+KALSMF++MLE G+RPNDLT+VSAL AC KIGAL+ G RIHNY   NG +LN+    ALVDMYAKCGNI SAS+VF ETK KD+ TWSVMIWGWAIHG + QA++CF KM  +GI PD V+FLA+LTACSHSG VD GLNFF+SMR DYSIEPTMKHYTL+VDLLGRAGRLDEA+ FIQ MPI+PDFV WGALFCACRAHKNI+MAEL +EKLL+LEPKHPGSYVFLSNVYAAVGRWEDVERVR LM+N+ V KDPGWSYIEV GQVHSFVAGDH H RA EI LKLEEI + A+++GYMPET WVLHNIEEEEKEDALGSHSEKLALAF L++T+PG+TIRIVKNLRVCGDCHS+MK ASK+S+REIILRDIKRFHHFK G CSCGDYW
Sbjct:   40 ETHFIPLIHASNTLPQLHQIHAQIFLHNLFSNSRVVTQLISSSCSLKSLDYALSIFRCFDHPNLFVFNALIRGLAENSRFEGSVSHFVLMLRLSIRPDRLTLPFVLKSVAALVDVGLGRCLHGGVMKLGLEFDSFVRVSLVDMYVKIGELGFGLQLFDESPQRNKAESILLWNVLINGCCKVGDLSKAASLFEAMPERNAGSWNSLINGFVRNGDLDRARELFVQMPEKNVVSWTTMINGFSQNGDHEKALSMFWRMLEEGVRPNDLTVVSALLACTKIGALQVGERIHNYLSSNGFQLNRGIGTALVDMYAKCGNIKSASRVFVETKGKDLLTWSVMIWGWAIHGCFDQALQCFVKMKSAGINPDEVIFLAILTACSHSGNVDQGLNFFESMRLDYSIEPTMKHYTLIVDLLGRAGRLDEALSFIQSMPINPDFVIWGALFCACRAHKNIEMAELTAEKLLQLEPKHPGSYVFLSNVYAAVGRWEDVERVRTLMKNRGVEKDPGWSYIEVEGQVHSFVAGDHAHVRAEEISLKLEEITASAKQEGYMPETAWVLHNIEEEEKEDALGSHSEKLALAFGLISTAPGSTIRIVKNLRVCGDCHSMMKYASKLSRREIILRDIKRFHHFKDGTCSCGDYW 677          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: A0A067KWK1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01110 PE=4 SV=1)

HSP 1 Score: 977.622 bits (2526), Expect = 0.000e+0
Identity = 460/642 (71.65%), Postives = 546/642 (85.05%), Query Frame = -3

Query:  591 QDPLKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2516
            Q+P +TH  SLI +++T+ QL QIHAQI   +LS+S+ + T LIS SSS K I YA+++FNH + K+ FLFNALIRGLT NS F+S++SHF+LMLR  V+PD+LT+PFVLKS+A L    LG  LHG I KSG EFD FVR+S+V+ YVK+EE+G AL++FDESP+R   ES LLWNVLINGCCKVG++ KA++LFE MPER   SWNS ING +++GDL +A +LF  M EK+VVSWTT+VNG+S NGDH+KALS+F KML+VG++PND T+VSALSACAKIGALEAGVRIH Y  +NG RLN     ALVDMYAKCG+I SAS+VF ETKEKD+ TW+VMIWGWAIHG   +AI+CF++MM++GI+PD VVFLA+LTAC+H+G+VDLGLNFF SM  DYSIEP+MKHY L+VDLLGRAGRL++A+KFI+RMPI+PDFV WGALFC CRAHKNIK+AEL ++KLL LEPKHPGSYVFLSNVYAAVGRWED ERVR LMQN+ + KDPGWSY+EV GQVHSF AGD  HK A++IYLKLE+IV+GA+ QGYMP T WVLHNIEEEEKEDALGSHSEKLALAF L+ TSPG T+RIVKNLRVCGDCHSLMK ASKMSQREIILRDIKRFHHFK G+CSCGDYW
Sbjct:   33 QNPSETHLISLIHASKTSRQLHQIHAQIFLHNLSTSSQIATQLISSSSSRKFIDYAITVFNHYYPKNSFLFNALIRGLTNNSLFESAISHFILMLRSDVKPDQLTYPFVLKSIATLCSEGLGRALHGMIYKSGFEFDLFVRISMVDAYVKVEELGSALKLFDESPQRFYGESTLLWNVLINGCCKVGSMRKAVDLFETMPERTTASWNSLINGFLRSGDLERANELFGRMPEKNVVSWTTMVNGLSHNGDHEKALSLFSKMLQVGVKPNDFTIVSALSACAKIGALEAGVRIHRYLTDNGFRLNAKIGTALVDMYAKCGSIESASQVFRETKEKDVLTWTVMIWGWAIHGHSEEAIQCFRQMMYAGIRPDEVVFLAILTACTHAGKVDLGLNFFKSMELDYSIEPSMKHYALIVDLLGRAGRLNQALKFIERMPITPDFVIWGALFCTCRAHKNIKLAELAAQKLLELEPKHPGSYVFLSNVYAAVGRWEDAERVRSLMQNRGIEKDPGWSYVEVEGQVHSFAAGDSSHKDAKDIYLKLEQIVAGAKGQGYMPGTEWVLHNIEEEEKEDALGSHSEKLALAFGLIRTSPGMTLRIVKNLRVCGDCHSLMKYASKMSQREIILRDIKRFHHFKDGICSCGDYW 674          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: B9GFV9_POPTR (Pentatricopeptide repeat-containing family protein OS=Populus trichocarpa GN=POPTR_0001s32380g PE=4 SV=1)

HSP 1 Score: 904.82 bits (2337), Expect = 0.000e+0
Identity = 435/641 (67.86%), Postives = 533/641 (83.15%), Query Frame = -3

Query:  591 PLKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPER-NKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2510
            P + HF SLI  ++T LQL QIHAQI+  +LSSS+ +TT LIS SS  KSI ++L++FNH   K+LF FNALIRGLT NSHF +++ HF LMLR  ++PD+LT+PFVLKS+AGL    LG+ +H  I++ G+E DSFVRVSLV+MYVK+E++G A +VFDESPER +   S LLWNVLI GCCK G+++KA++LF+AMP++   SW++ I+G  KNGD+++AM+LFD+M EK+VVSWTT+V+G S+NGD +KALSMF KMLE G+RPN  T+VSALSACAKIG LEAG+RIH Y  +NGL L +A   ALVDMYAKCGNI SAS+VF ET++K IRTW+VMIWGWAIHG   QAI CFK+MMF+GIKPD VVFLALLTAC HSGQVD+GLNFFDSMR DY IEP+MKHYTL+VD+LGR+G+L EA++FI+RMP++PDFV WGALFCACRAHK  KMA+    KLL+LEP H G+Y+FLSN YAA+G+WED ERVR+LMQN+ V K+ GWS IEV GQVH FV+GDHDHK ++ I LKLEEI++GA +QGY+P T WVLHN+E+EEKED LGSH EKLALAFAL+ TSPG TIRIVKNL+VCGDCHSLMK ASK+SQREI+LRD+KRFHHFK G CSC D+W
Sbjct:   79 PTEAHFISLIHGSKTILQLHQIHAQIIIHNLSSSSLITTQLISSSSLRKSINHSLAVFNHHKPKNLFTFNALIRGLTTNSHFFNAIFHFRLMLRSGIKPDRLTYPFVLKSMAGLFSTELGMAIHCMILRCGIELDSFVRVSLVDMYVKVEKLGSAFKVFDESPERFDSGSSALLWNVLIKGCCKAGSMKKAVKLFKAMPKKENVSWSTLIDGFAKNGDMDRAMELFDQMPEKNVVSWTTMVDGFSRNGDSEKALSMFSKMLEEGVRPNAFTIVSALSACAKIGGLEAGLRIHKYIKDNGLHLTEALGTALVDMYAKCGNIESASEVFGETEQKSIRTWTVMIWGWAIHGHSEQAIACFKQMMFAGIKPDEVVFLALLTACMHSGQVDIGLNFFDSMRLDYCIEPSMKHYTLIVDMLGRSGQLKEALRFIERMPMNPDFVIWGALFCACRAHKKTKMAKFALNKLLKLEPTHTGNYIFLSNAYAALGQWEDAERVRVLMQNRGVHKNSGWSCIEVEGQVHRFVSGDHDHKDSKAICLKLEEIMAGAVKQGYIPGTEWVLHNMEQEEKEDVLGSHGEKLALAFALICTSPGMTIRIVKNLQVCGDCHSLMKYASKISQREIMLRDMKRFHHFKDGSCSCRDHW 719          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: K4CWN1_SOLLC (Uncharacterized protein OS=Solanum lycopersicum GN=Solyc09g092050.2 PE=4 SV=1)

HSP 1 Score: 879.396 bits (2271), Expect = 0.000e+0
Identity = 426/647 (65.84%), Postives = 518/647 (80.06%), Query Frame = -3

Query:  567 KTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEM-KEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW*NGSSVRL 2504
            ++HF SLI S+   LQL+QIH QI+R++ SS++ + T LIS +S  KSI Y LSIF+    K++FLFN LIRGL ENS F+ S+ +F  M++  VRPDKLT+PFVLKSV  LG + +G ++H  I+K G+E+D+FVRV LVEMYVK E + FALQ+FDES ERNK ES++LWNV+INGCCK+G + KA+ LFE MPERN+GSWN+ I+GL++NG+++KAM+LFDEM  EK+VVSWT +++G+  N  HQKAL +FFKM+E G++PN LT+VSALSACAK GALEAG +IH+  V NGL LN A   AL+DMYAKCG I SAS VF   KEKDIRTWS+MIWGWAIHG   +A+RCF++M  +GIKPD V  LA+LT CSH+G+VD GL  FD M+  +SIEPTMKHY  VVDLLGRAGR DEA+KFI  MP+ PD+V WGALF ACRAHKNI+MA++ SEKLL+LEPKH G YVFLSNVYA  GRW+DVERVR  M+N+ V KDPGWS +EV GQ+H+FVAGD  H R +EIYLKLEEI++GA++QGYMPET WVLHNI+EEEKE ALGSHSEKLALAF L++T PG  I IVKNLRVCGDCHSLMK  S+MSQR I+LRDIKRFHHFK GVCSC DYW   S   L
Sbjct:   28 ESHFISLIHSSRNILQLQQIHGQIIRKNFSSNSRIVTQLISSASLHKSINYGLSIFSCFLDKNVFLFNVLIRGLKENSLFEKSILYFRKMVKMGVRPDKLTYPFVLKSVTALGDKRVGGVVHCGILKMGLEYDTFVRVCLVEMYVKAELVDFALQLFDESSERNKVESVILWNVVINGCCKIGRVSKALALFEEMPERNVGSWNTLISGLLRNGEVDKAMELFDEMTNEKNVVSWTCMIHGLMLNELHQKALDLFFKMVEEGVKPNGLTVVSALSACAKTGALEAGKKIHDNIVNNGLHLNAAVGNALLDMYAKCGYIESASLVFSGLKEKDIRTWSIMIWGWAIHGHVDKALRCFEQMRLTGIKPDGVSVLAVLTGCSHAGRVDQGLQIFDGMQRQFSIEPTMKHYAAVVDLLGRAGRFDEALKFIASMPLEPDYVIWGALFSACRAHKNIEMAKVASEKLLQLEPKHAGGYVFLSNVYAGAGRWDDVERVRSSMKNKNVEKDPGWSSMEVAGQLHTFVAGDSAHTRKQEIYLKLEEIITGAKQQGYMPETEWVLHNIDEEEKEGALGSHSEKLALAFGLISTGPGVIIMIVKNLRVCGDCHSLMKYVSRMSQRVIVLRDIKRFHHFKDGVCSCKDYWSQASDTYL 674          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: A0A0A0LI86_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G139850 PE=4 SV=1)

HSP 1 Score: 858.21 bits (2216), Expect = 0.000e+0
Identity = 426/639 (66.67%), Postives = 517/639 (80.91%), Query Frame = -3

Query:  591 LKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2507
            L+THF  LI ++ +T +LRQIH Q+ R ++ SS+ + T  IS  SSL S+ YA+SIF     K+ +LFNALIRGL ENS F+SS+S F+LML+ ++ PD+LTFPFVLKS A L    +G  LH  I+K G+EFDSFVRVSLV+MYVK+EE+G AL+VFDESPE  K+ S+L+WNVLI+G C++G+L KA ELF++MP+++ GSWNS ING MK GD+ +A +LF +M EK+VVSWTT+VNG SQNGD +KAL  FF MLE G RPND T+VSALSACAKIGAL+AG+RIHNY   NG +LN     ALVDMYAKCGNI  A KVF ETKEK +  WSVMIWGWAIHG + +A++ F+ M F+G KPD+VVFLA+L ACSHSGQV+ GL FFD+MR  Y IEP+MKHYTLVVD+LGRAGRLDEA+KFI+ MPI+PDFV WGALFCACR HKN++MAEL S+KLL+LEPKHPGSYVFLSN YA+VGRW+D ERVR+ M++    KDPGWS+IEV+ ++H FVAGD+ H RA EIY KL+EI + ARE+GY  E   VLHNIEEEEKE+ALG HSEKLALAF +++T PGTT+RIVKNLRVC DCHS MK ASKMS+REIILRD+KRFHHF  GVCSCGDYW
Sbjct:   41 LETHFIDLIHASNSTHKLRQIHGQLYRCNVFSSSRVVTQFISSCSSLNSVDYAISIFQRFELKNSYLFNALIRGLAENSRFESSISFFVLMLKWKISPDRLTFPFVLKSAAALSNGGVGRALHCGILKFGLEFDSFVRVSLVDMYVKVEELGSALKVFDESPESVKNGSVLIWNVLIHGYCRMGDLVKATELFDSMPKKDTGSWNSLINGFMKMGDMGRAKELFVKMPEKNVVSWTTMVNGFSQNGDPEKALETFFCMLEEGARPNDYTIVSALSACAKIGALDAGLRIHNYLSGNGFKLNLVIGTALVDMYAKCGNIEHAEKVFHETKEKGLLIWSVMIWGWAIHGHFRKALQYFEWMKFTGTKPDSVVFLAVLNACSHSGQVNEGLKFFDNMRRGYLIEPSMKHYTLVVDMLGRAGRLDEALKFIRAMPITPDFVVWGALFCACRTHKNVEMAELASKKLLQLEPKHPGSYVFLSNAYASVGRWDDAERVRVSMRDHGAHKDPGWSFIEVDHKLHRFVAGDNTHNRAVEIYSKLDEISASAREKGYTKEIECVLHNIEEEEKEEALGYHSEKLALAFGIVSTRPGTTVRIVKNLRVCVDCHSFMKYASKMSKREIILRDMKRFHHFNDGVCSCGDYW 679          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: V4S5Y2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10004495mg PE=4 SV=1)

HSP 1 Score: 837.41 bits (2162), Expect = 0.000e+0
Identity = 418/638 (65.52%), Postives = 510/638 (79.94%), Query Frame = -3

Query:  591 KTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2504
            +TH  SLI S+ +T QLRQIHAQI+  +L +S+ +TT LIS +S  KS  YALSIF H   K+L +FN LIRGL ENSHFQS +SHF+ MLR  VRP++LT+PFV KSVA L L  LG  LH  I+KSGVE+D+FVRV L +MYV+L +   A +VFDE+PE+NKSES+LLWNVLINGC K+G L KA+ELF  MP++N+ SW S I+G M+ GDL KA +LF++M EK VVSWT ++NG SQNG+ +KAL+MFF+ML+ G+R ND T+VSALSACAK+GALEAGVR+HNY   N   L  A   ALVDMYAKCGNI +AS VF ETKEKD+ TW+ MIWG AIHG Y QAI+ FKKMM+SG +PD  VFLA+LTAC +SGQV L LNFFDSMRFDY IEP++KH+T+VV+LL R G++D+A+ FI +MP +PDFV WGALFCACR HK+ K+A++  ++LL+L+PKHP SYV LSN+YAA GRW+DV RVR LMQ +++ KDPGWSYIEVNG VH F AG   HK A+EI+ KLEEI++GAREQGYMP T WVLHNI +EEKE+ALG HSEKLALAF L+ T+PGTTI+IVK L +CGDCHSLMK ASK+SQREI+LRD  RFH+FK G CSC DYW
Sbjct:   31 ETHIISLIHSSNSTKQLRQIHAQIILHNLFASSRITTQLISSASLHKSTDYALSIFGHFTPKNLHIFNVLIRGLAENSHFQSCISHFVFMLRLSVRPNRLTYPFVSKSVASLSLLSLGRGLHCLIVKSGVEYDAFVRVHLADMYVQLGKTRGAFKVFDETPEKNKSESVLLWNVLINGCSKIGYLRKAVELFGMMPKKNVASWVSLIDGFMRKGDLKKAGELFEQMPEKGVVSWTAMINGFSQNGEAEKALAMFFQMLDAGVRANDFTVVSALSACAKVGALEAGVRVHNYISCNDFGLKGAIGTALVDMYAKCGNIEAASLVFGETKEKDLLTWTAMIWGLAIHGRYEQAIQYFKKMMYSGTEPDGTVFLAILTACWYSGQVKLALNFFDSMRFDYFIEPSVKHHTVVVNLLSRVGQVDKALNFINKMPETPDFVIWGALFCACRTHKDTKIAKIALQRLLQLKPKHPSSYVLLSNIYAAEGRWKDVARVRTLMQRRSIKKDPGWSYIEVNGHVHRFEAG--GHKLAKEIHSKLEEIMAGAREQGYMPGTEWVLHNI-KEEKEEALGCHSEKLALAFGLIQTTPGTTIKIVKKLTICGDCHSLMKYASKISQREIVLRDT-RFHYFKDGTCSCRDYW 664          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: A0A068US57_COFCA (Coffea canephora DH200=94 genomic scaffold, scaffold_52 OS=Coffea canephora GN=GSCOC_T00033183001 PE=4 SV=1)

HSP 1 Score: 820.461 bits (2118), Expect = 0.000e+0
Identity = 382/550 (69.45%), Postives = 460/550 (83.64%), Query Frame = -3

Query:  591 MLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEV-GLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2237
            M R  VRP +LTFPFVLKSV GLG ++LG ++HG I+K G+EFD FVRVSLV+MY K+E +  ALQ+FDESPERNK +S+LLWNV+INGCCK G LEKA+ELFEAMPERN+GSWNS INGL++NG ++KA++LF+ M EK+VVSWTT+V+G+S NG H+KAL MFF M+E  G+R NDLTLVS LSACAK GALEAG++IH Y + N  RLN A   AL+DMYAKCG I SAS+VF +TKEKD+RTWSVMIWGWAIHG   QA++CF+KM  +GI+PD V FLA+LTACSH+G VD GL FFDSM+ DYSIEPTMKH  ++VDL GRAG+LDEA++FIQ M ++PDFV WGALF ACRAHKNI+MA+ VSEKLL+LEPKH GSYVF+SN+YA VG WE+VERVR  M+++   KD G S++EV+G++HSFVAGD  H   +EIY KLEE+ + ARE GY+PET WVLHNIEEEEKEDALG+HSEKLALAF L++T PG  +RIVKNLR CGDCHSLMK  SK+SQREI++RDIKRFHHFK G+CSC DYW
Sbjct:    1 MSRLDVRPSRLTFPFVLKSVVGLGDKWLGGMVHGGILKMGLEFDGFVRVSLVDMYAKMELLSLALQLFDESPERNKLDSVLLWNVVINGCCKSGILEKAVELFEAMPERNLGSWNSLINGLIRNGKVDKAVELFEGMVEKNVVSWTTMVHGLSLNGMHEKALEMFFNMIEEEGVRANDLTLVSVLSACAKTGALEAGIKIHKYILGNKFRLNAAVGTALIDMYAKCGQIQSASQVFRDTKEKDVRTWSVMIWGWAIHGSVEQALQCFEKMKLTGIEPDEVAFLAVLTACSHAGLVDEGLKFFDSMKLDYSIEPTMKHCAVIVDLYGRAGQLDEALRFIQCMALTPDFVIWGALFSACRAHKNIEMAKYVSEKLLQLEPKHSGSYVFMSNIYAGVGIWEEVERVRTSMKDKGAAKDTGLSHVEVHGKLHSFVAGDQAHMHTKEIYSKLEEMTNRAREHGYIPETEWVLHNIEEEEKEDALGTHSEKLALAFGLISTGPGVVLRIVKNLRTCGDCHSLMKHVSKLSQREIVVRDIKRFHHFKDGICSCQDYW 550          

HSP 2 Score: 70.8626 bits (172), Expect = 2.825e-9
Identity = 69/293 (23.55%), Postives = 134/293 (45.73%), Query Frame = -3

Query: 1503 ALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQR-VRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMP----ERNIGSWNSFINGLMKNGDLNKAMQLFDEMK-----EKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRT 2351
            A+ +F     K++  +  ++ GL+ N   + ++  F  M+ +  VR + LT   VL + A  G    G+ +H  I+ +    ++ V  +L++MY K  ++  A QVF ++ E++    +  W+V+I G    G++E+A++ FE M     E +  ++ + +      G +++ ++ FD MK     E  +     IV+   + G   +AL     M    L P+ +   +  SAC         + +  Y  E  L+L    + + V M     NI +   ++EE +   +RT
Sbjct:  130 AVELFEGMVEKNVVSWTTMVHGLSLNGMHEKALEMFFNMIEEEGVRANDLTLVSVLSACAKTGALEAGIKIHKYILGNKFRLNAAVGTALIDMYAKCGQIQSASQVFRDTKEKD----VRTWSVMIWGWAIHGSVEQALQCFEKMKLTGIEPDEVAFLAVLTACSHAGLVDEGLKFFDSMKLDYSIEPTMKHCAVIVDLYGRAGQLDEALRFIQCM---ALTPDFVIWGALFSACRA----HKNIEMAKYVSEKLLQLEPKHSGSYVFM----SNIYAGVGIWEEVER--VRT 405          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: M1CI35_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG401026412 PE=4 SV=1)

HSP 1 Score: 801.201 bits (2068), Expect = 0.000e+0
Identity = 376/545 (68.99%), Postives = 453/545 (83.12%), Query Frame = -3

Query:  591 VRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEM-KEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2222
            VRPDKLT+PFVLKSV  LG + +G  +H  ++K G+E+D+FVRV LVE+YVK+E + FALQ+FDESPERNK ES++LWNV+INGCCK+G +  A+ LFE MPERN+GSWN+ I+GL++NG+++KAM+LFDEM  EK+VVSWT +++G+  NG HQKAL +FFKM+E G++PN LT+VSALSACAK GALEAG +IH+  + NGL LN A   AL+DMYAKCG I SAS VF   KEKDIRTWS+MIWGWAIHG   +A+RCF++M  +GIKPD V  LA+LT CSH+G+VD GL  FDSM+ + SIEPTMKHY  VVDLLGRAGR DEA+KFI  MP+ PD+V WGALF ACRAHKNI+MA++ SEKLL+LEPKH G YVFLSNVYA  GRW+DVERVR  M+N+ V KDPGWS +EV+GQ+H+FVAGD  H R +EIYLKLEEI++GA++QGYMPET WVLHNI+EEEKE ALGSHSEKLALAF L++T PG  I IVKNLRVCGDCHSLMK  S+MSQR I+LRDIKRFHHFK GVCSC DYW
Sbjct:    3 VRPDKLTYPFVLKSVTALGEKGVGGGVHCGVLKVGLEYDTFVRVCLVELYVKVELVDFALQLFDESPERNKVESVILWNVVINGCCKIGRMSNALALFEEMPERNVGSWNTLISGLLRNGEVDKAMELFDEMPNEKNVVSWTCMIHGLMLNGLHQKALDLFFKMVEEGVKPNGLTVVSALSACAKTGALEAGKKIHDNIMNNGLHLNAAVGNALLDMYAKCGYIESASLVFSGLKEKDIRTWSIMIWGWAIHGDVDKALRCFEQMRLTGIKPDGVSVLAVLTGCSHAGRVDQGLQIFDSMQHECSIEPTMKHYAAVVDLLGRAGRFDEALKFIASMPLEPDYVIWGALFSACRAHKNIEMAKVASEKLLQLEPKHAGGYVFLSNVYAGAGRWDDVERVRSSMKNKNVEKDPGWSSMEVDGQLHTFVAGDSAHTRKQEIYLKLEEIITGAKQQGYMPETEWVLHNIDEEEKEGALGSHSEKLALAFGLISTGPGVIIMIVKNLRVCGDCHSLMKYVSRMSQRVIVLRDIKRFHHFKDGVCSCKDYW 547