g.barbadense_cottongen_reftransV1_0097138, g.barbadense_cottongen_reftransV1_0097138 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PP295_ARATH (Pentatricopeptide repeat-containing protein At3g62890 OS=Arabidopsis thaliana GN=PCMP-H82 PE=2 SV=1)

HSP 1 Score: 443.351 bits (1139), Expect = 9.375e-143
Identity = 236/599 (39.40%), Postives = 348/599 (58.10%), Query Frame = -3

Query:  591 YALSIFNHSHHK-SLFLFNALIRGLTEN---SHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKML-----EVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEET-KEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFS-GIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2354
            YA  IF+  H K   FL+N +IR +  N       S +S +L M   RV PD  TFPF+L S        LG   H +I+  G++ D FVR SL+ MY                                + C   G+L  A  +F+    +++ +WNS +N   K G ++ A +LFDEM E++V+SW+ ++NG    G +++AL +F +M      E  +RPN+ T+ + LSAC ++GALE G  +H Y  +  + ++     AL+DMYAKCG++  A +VF     +KD++ +S MI   A++G   +  + F +M  S  I P++V F+ +L AC H G ++ G ++F  M  ++ I P+++HY  +VDL GR+G + EA  FI  MP+ PD + WG+L    R   +IK  E   ++L+ L+P + G+YV LSNVYA  GRW +V+ +R  M+ + + K PG SY+EV G VH FV GD   + +  IY  L+EI+   RE GY+ +T  VL ++ E++KE AL  HSEKLA+AF LM T PGT +RI+KNLR+CGDCH +MK  SK+  REI++RD  RFHHF+ G CSC D+W
Sbjct:   10 YANPIFHIRHLKLESFLWNIIIRAIVHNVSSPQRHSPISVYLRMRNHRVSPDFHTFPFLLPSFHNPLHLPLGQRTHAQILLFGLDKDPFVRTSLLNMY--------------------------------SSC---GDLRSAQRVFDDSGSKDLPAWNSVVNAYAKAGLIDDARKLFDEMPERNVISWSCLINGYVMCGKYKEALDLFREMQLPKPNEAFVRPNEFTMSTVLSACGRLGALEQGKWVHAYIDKYHVEIDIVLGTALIDMYAKCGSLERAKRVFNALGSKKDVKAYSAMICCLAMYGLTDECFQLFSEMTTSDNINPNSVTFVGILGACVHRGLINEGKSYFKMMIEEFGITPSIQHYGCMVDLYGRSGLIKEAESFIASMPMEPDVLIWGSLLSGSRMLGDIKTCEGALKRLIELDPMNSGAYVLLSNVYAKTGRWMEVKCIRHEMEVKGINKVPGCSYVEVEGVVHEFVVGDESQQESERIYAMLDEIMQRLREAGYVTDTKEVLLDLNEKDKEIALSYHSEKLAIAFCLMKTRPGTPVRIIKNLRICGDCHLVMKMISKLFSREIVVRDCNRFHHFRDGSCSCRDFW 573          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PP301_ARATH (Pentatricopeptide repeat-containing protein At4g02750 OS=Arabidopsis thaliana GN=PCMP-H24 PE=3 SV=1)

HSP 1 Score: 449.514 bits (1155), Expect = 1.348e-142
Identity = 234/610 (38.36%), Postives = 354/610 (58.03%), Query Frame = -3

Query:  591 ALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHF-----------------LLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDS------FVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2351
            A S+F+    K+   +NAL+    +NS  + +   F                  +  ++ V   +      ++ V        G    G+I ++   FD       F   ++V  Y++   +  A ++FD+ PERN+    + WN ++ G  +   +E A ELF+ MP RN+ +WN+ I G  + G +++A  LFD+M ++D VSW  ++ G SQ+G   +AL +F +M   G R N  +  SALS CA + ALE G ++H   V+ G         AL+ MY KCG+I  A+ +F+E   KDI +W+ MI G++ HGF   A+R F+ M   G+KPD    +A+L+ACSH+G VD G  +F +M  DY + P  +HY  +VDLLGRAG L++A   ++ MP  PD   WG L  A R H N ++AE  ++K+  +EP++ G YV LSN+YA+ GRW DV ++R+ M+++ V K PG+S+IE+  + H+F  GD  H    EI+  LEE+    ++ GY+ +T  VLH++EEEEKE  +  HSE+LA+A+ +M  S G  IR++KNLRVC DCH+ +K  ++++ R IILRD  RFHHFK G CSCGDYW
Sbjct:  176 ARSVFDRMPEKNDVSWNALLSAYVQNSKMEEACMLFKSRENWALVSWNCLLGGFVKKKKIVEARQFFDSMNVRDVVSWNTIITGYAQSGKIDEARQLFDESPVQDVFTWTAMVSGYIQNRMVEEARELFDKMPERNE----VSWNAMLAGYVQGERMEMAKELFDVMPCRNVSTWNTMITGYAQCGKISEAKNLFDKMPKRDPVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRLNRSSFSSALSTCADVVALELGKQLHGRLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFKEMAGKDIVSWNTMIAGYSRHGFGEVALRFFESMKREGLKPDDATMVAVLSACSHTGLVDKGRQYFYTMTQDYGVMPNSQHYACMVDLLGRAGLLEDAHNLMKNMPFEPDAAIWGTLLGASRVHGNTELAETAADKIFAMEPENSGMYVLLSNLYASSGRWGDVGKLRVRMRDKGVKKVPGYSWIEIQNKTHTFSVGDEFHPEKDEIFAFLEELDLRMKKAGYVSKTSVVLHDVEEEEKERMVRYHSERLAVAYGIMRVSSGRPIRVIKNLRVCEDCHNAIKYMARITGRLIILRDNNRFHHFKDGSCSCGDYW 781          

HSP 2 Score: 125.946 bits (315), Expect = 3.082e-28
Identity = 88/321 (27.41%), Postives = 154/321 (47.98%), Query Frame = -3

Query: 1173 RIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCA 2135
            R+ K    + S     ++  Y++  E   A ++FDE PER+    ++ WNV+I G  +  NL KA ELFE MPER++ SWN+ ++G  +NG ++ A  +FD M EK+ VSW  +++   QN   ++A  M FK  E      +  LVS           +  V    +F    +R +  +   ++  YA+ G I  A ++F+E+  +D+ TW+ M+ G+  +    +A   F KM     + + V + A+L       ++++    FD M         +  +  ++    + G++ EA     +MP   D V+W A+   
Sbjct:   85 RVFKRMPRWSSVSYNGMISGYLRNGEFELARKLFDEMPERD----LVSWNVMIKGYVRNRNLGKARELFEIMPERDVCSWNTMLSGYAQNGCVDDARSVFDRMPEKNDVSWNALLSAYVQNSKMEEAC-MLFKSRE------NWALVSWNCLLGGFVKKKKIVEARQFFDSMNVR-DVVSWNTIITGYAQSGKIDEARQLFDESPVQDVFTWTAMVSGYIQNRMVEEARELFDKM----PERNEVSWNAMLAGYVQGERMEMAKELFDVMPC-----RNVSTWNTMITGYAQCGKISEAKNLFDKMP-KRDPVSWAAMIAG 383          

HSP 3 Score: 90.8929 bits (224), Expect = 2.529e-17
Identity = 70/287 (24.39%), Postives = 130/287 (45.30%), Query Frame = -3

Query: 1140 WNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIK-PDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLG--RAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAE 1991
            WNV I+   + G   +A+ +F+ MP  +  S+N  I+G ++NG+   A +LFDEM E+D+VSW  ++ G  +N +  KA  +F  M E  +     +  + LS  A+ G ++    + +   E     N  +  AL+  Y +   +  A  +F     K    W+++ W   + GF  +      +  F  +   D V +  ++T  + SG++D     FD         P    +T    + G  +   ++EA +   +MP   + V+W A+       + ++MA+
Sbjct:   67 WNVAISSYMRTGRCNEALRVFKRMPRWSSVSYNGMISGYLRNGEFELARKLFDEMPERDLVSWNVMIKGYVRNRNLGKARELFEIMPERDV----CSWNTMLSGYAQNGCVDDARSVFDRMPEK----NDVSWNALLSAYVQNSKMEEACMLF-----KSRENWALVSWNCLLGGFVKKKKIVEARQFFDSMNVRDVVSWNTIITGYAQSGKIDEARQLFDE-------SPVQDVFTWTAMVSGYIQNRMVEEARELFDKMP-ERNEVSWNAMLAGYVQGERMEMAK 332          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PP223_ARATH (Putative pentatricopeptide repeat-containing protein At3g11460 OS=Arabidopsis thaliana GN=PCMP-H52 PE=3 SV=1)

HSP 1 Score: 441.81 bits (1135), Expect = 1.242e-141
Identity = 235/611 (38.46%), Postives = 338/611 (55.32%), Query Frame = -3

Query:  591 FNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGS------------------W---------------------NSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2306
            +N  +R L   S F  S+S +  MLR    PD  +FPF+LKS A L L   G  LH  + K G E + FV  +L+ MY K   +  A +VF+E+P+   S+  + +N LI+G      +  A  +F  M E  +                    W                     NSFI   MK G +    +LFDEM  K +++W  +++G SQNG     L ++ +M   G+ P+  TLVS LS+CA +GA + G  +      NG   N   + A + MYA+CGN+  A  VF+    K + +W+ MI  + +HG     +  F  M+  GI+PD  VF+ +L+ACSHSG  D GL  F +M+ +Y +EP  +HY+ +VDLLGRAGRLDEAM+FI+ MP+ PD   WGAL  AC+ HKN+ MAEL   K++  EP + G YV +SN+Y+     E + R+R++M+ +A  K PG+SY+E  G+VH F+AGD  H++  E++  L+E+     E   M E    +     EE       HSE+LA+AF ++N+ PGT I ++KNLRVC DCH  +K  SK+  R+ ++RD  RFH+FK GVCSC DYW
Sbjct:   21 WNVRLRELAYQSLFSESISLYRSMLRSGSSPDAFSFPFILKSCASLSLPVSGQQLHCHVTKGGCETEPFVLTALISMYCKCGLVADARKVFEENPQ--SSQLSVCYNALISGYTANSKVTDAAYMFRRMKETGVSVDSVTMLGLVPLCTVPEYLWLGRSLHGQCVKGGLDSEVAVLNSFITMYMKCGSVEAGRRLFDEMPVKGLITWNAVISGYSQNGLAYDVLELYEQMKSSGVCPDPFTLVSVLSSCAHLGAKKIGHEVGKLVESNGFVPNVFVSNASISMYARCGNLAKARAVFDIMPVKSLVSWTAMIGCYGMHGMGEIGLMLFDDMIKRGIRPDGAVFVMVLSACSHSGLTDKGLELFRAMKREYKLEPGPEHYSCLVDLLGRAGRLDEAMEFIESMPVEPDGAVWGALLGACKIHKNVDMAELAFAKVIEFEPNNIGYYVLMSNIYSDSKNQEGIWRIRVMMRERAFRKKPGYSYVEHKGRVHLFLAGDRSHEQTEEVHRMLDEL-----ETSVM-ELAGNMDCDRGEEVSSTTREHSERLAIAFGILNSIPGTEILVIKNLRVCEDCHVFLKQVSKIVDRQFVVRDASRFHYFKDGVCSCKDYW 623          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PP271_ARATH (Putative pentatricopeptide repeat-containing protein At3g49142 OS=Arabidopsis thaliana GN=PCMP-H77 PE=3 SV=1)

HSP 1 Score: 444.121 bits (1141), Expect = 1.320e-141
Identity = 239/633 (37.76%), Postives = 355/633 (56.08%), Query Frame = -3

Query:  591 LRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNI----GSWNSFINGLMKNGDLN--KAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNT-----SPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2456
            LR +H++I+   L  +++L   L+   +SLK +  A  +F+    +++ + N +IR    N  +   V  F  M    VRPD  TFP VLK+ +  G   +G  +HG   K G+    FV   LV MY K   +  A  V DE   R+    ++ WN L+ G  +    + A+E+   M    I    G+  S +  +      N      +F +M +K +VSW  ++    +N    +A+ ++ +M   G  P+ +++ S L AC    AL  G +IH Y     L  N     AL+DMYAKCG +  A  VFE  K +D+ +W+ MI  +   G    A+  F K+  SG+ PD++ F+  L ACSH+G ++ G + F  M   Y I P ++H   +VDLLGRAG++ EA +FIQ M + P+   WGAL  ACR H +  +  L ++KL +L P+  G YV LSN+YA  GRWE+V  +R +M+++ + K+PG S +EVN  +H+F+ GD  H ++ EIY +L+ +V   +E GY+P++   LH++EEE+KE  L  HSEKLA+ FALMNT         TIRI KNLR+CGDCH   K  S+++ REII+RD  RFH F++GVCSCGDYW
Sbjct:   58 LRTVHSRIILEDLRCNSSLGVKLMRAYASLKDVASARKVFDEIPERNVIIINVMIRSYVNNGFYGEGVKVFGTMCGCNVRPDHYTFPCVLKACSCSGTIVIGRKIHGSATKVGLSSTLFVGNGLVSMYGKCGFLSEARLVLDEMSRRD----VVSWNSLVVGYAQNQRFDDALEVCREMESVKISHDAGTMASLLPAVSNTTTENVMYVKDMFFKMGKKSLVSWNVMIGVYMKNAMPVEAVELYSRMEADGFEPDAVSITSVLPACGDTSALSLGKKIHGYIERKKLIPNLLLENALIDMYAKCGCLEKARDVFENMKSRDVVSWTAMISAYGFSGRGCDAVALFSKLQDSGLVPDSIAFVTTLAACSHAGLLEEGRSCFKLMTDHYKITPRLEHLACMVDLLGRAGKVKEAYRFIQDMSMEPNERVWGALLGACRVHSDTDIGLLAADKLFQLAPEQSGYYVLLSNIYAKAGRWEEVTNIRNIMKSKGLKKNPGASNVEVNRIIHTFLVGDRSHPQSDEIYRELDVLVKKMKELGYVPDSESALHDVEEEDKETHLAVHSEKLAIVFALMNTKEEEEDSNNTIRITKNLRICGDCHVAAKLISQITSREIIIRDTNRFHVFRFGVCSCGDYW 686          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PP285_ARATH (Pentatricopeptide repeat-containing protein At3g57430, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H81 PE=2 SV=2)

HSP 1 Score: 446.432 bits (1147), Expect = 4.744e-140
Identity = 242/632 (38.29%), Postives = 364/632 (57.59%), Query Frame = -3

Query:  591 KSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSG-VEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPE----------------------------------------RNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKM--LE---------VGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFV---AWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2321
            + L  +N ++  L +N     ++ +   M+ + V PD+ T   VL + + L +   G  LH   +K+G ++ +SFV  +LV+MY   +++    +VFD   +R     I LWN +I G  +  + ++A+ LF  M E                                        R+    N+ ++   + G ++ AM++F +M+++D+V+W T++ G   +  H+ AL +  KM  LE         V L+PN +TL++ L +CA + AL  G  IH Y ++N L  + A  +ALVDMYAKCG +  + KVF++  +K++ TW+V+I  + +HG   +AI   + MM  G+KP+ V F+++  ACSHSG VD GL  F  M+ DY +EP+  HY  VVDLLGRAGR+ EA + +  MP   DF    AW +L  A R H N+++ E+ ++ L++LEP     YV L+N+Y++ G W+    VR  M+ Q V K+PG S+IE   +VH FVAGD  H ++ ++   LE +    R++GY+P+T  VLHN+EE+EKE  L  HSEKLA+AF ++NTSPGT IR+ KNLRVC DCH   K  SK+  REIILRD++RFH FK G CSCGDYW
Sbjct:  265 RDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRK----IGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMP--RDFNKAGAWSSLLGASRIHNNLEIGEIAAQNLIQLEPNVASHYVLLANIYSSAGLWDKATEVRRNMKEQGVRKEPGCSWIEHGDEVHKFVAGDSSHPQSEKLSGYLETLWERMRKEGYVPDTSCVLHNVEEDEKEILLCGHSEKLAIAFGILNTSPGTIIRVAKNLRVCNDCHLATKFISKIVDREIILRDVRRFHRFKNGTCSCGDYW 890          

HSP 2 Score: 147.517 bits (371), Expect = 6.607e-35
Identity = 122/447 (27.29%), Postives = 200/447 (44.74%), Query Frame = -3

Query: 1104 LIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSF-VRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNI------------------------------------GSWNSF-INGLM----KNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENG-LRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFS-GIKPDAVVFLALLTACSHSGQVDL-----GLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPK 2297
            L+R    ++  + +V  ++ M+   ++PD   FP +LK+VA L    LG  +H  + K G   DS  V  +LV +Y K  + G   +VFD   ERN+    + WN LI+  C     E A+E F  M + N+                                    G  NSF IN L+    K G L  +  L      +D+V+W T+++ + QN    +AL    +M+  G+ P++ T+ S L AC+ +  L  G  +H Y ++NG L  N    +ALVDMY  C  +LS  +VF+   ++ I  W+ MI G++ +    +A+  F  M  S G+  ++     ++ AC  SG         G      +  D  ++ T+      +D+  R G++D AM+   +M    D V W  +       ++ + A L+  K+  LE K
Sbjct:   68 LLRSKVRSNLLREAVLTYVDMIVLGIKPDNYAFPALLKAVADLQDMELGKQIHAHVYKFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQ----VSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKGELNSFIINTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTL------MDMYSRLGKIDIAMRIFGKME-DRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERK 503          

HSP 3 Score: 115.546 bits (288), Expect = 6.125e-25
Identity = 104/483 (21.53%), Postives = 209/483 (43.27%), Query Frame = -3

Query: 1062 IFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLR---FLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLM--------------------KNGDLNK--------------------AMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLE-VGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMM-----------FSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMA-ELVSEKLLRLEPKHPGSYVFLSNVYAA 2342
            +F+    ++   +N+LI  L     ++ ++  F  ML + V P   T   V+ + + L +     +G  +H   ++ G E +SF+  +LV MY KL ++  +  +      R+    ++ WN +++  C+   L +A+E    M    +      I+ ++                    KNG L++                      ++FD M ++ +  W  ++ G SQN   ++AL +F  M E  GL  N  T+   + AC + GA      IH + V+ GL  ++     L+DMY++ G I  A ++F + +++D+ TW+ MI G+     +  A+    KM               +KP+++  + +L +C+    +  G     +     ++   +   + +VD+  + G L  + K   ++P   + + W  +  A   H N + A +L+  +++ ++   P    F+S V+AA
Sbjct:  155 VFDRISERNQVSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKG-ELNSFIINTLVAMYGKLGKLASSKVLLGSFGGRD----LVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIH-AYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIP-QKNVITWNVIIMAYGMHGNGQEAIDLL--RMMMVQGVKPNEVTFIS-VFAA 627          

HSP 4 Score: 68.9366 bits (167), Expect = 1.659e-10
Identity = 35/147 (23.81%), Postives = 79/147 (53.74%), Query Frame = -3

Query: 1368 WTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAA-ALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSH 1805
            W  ++    ++   ++A+  +  M+ +G++P++    + L A A +  +E G +IH +  + G  ++  T A  LV++Y KCG+  +  KVF+   E++  +W+ +I        +  A+  F+ M+   ++P +   ++++TACS+
Sbjct:   65 WIDLLRSKVRSNLLREAVLTYVDMIVLGIKPDNYAFPALLKAVADLQDMELGKQIHAHVYKFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSN 211          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PPR32_ARATH (Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H40 PE=2 SV=1)

HSP 1 Score: 439.884 bits (1130), Expect = 1.596e-138
Identity = 247/685 (36.06%), Postives = 385/685 (56.20%), Query Frame = -3

Query:  591 DPLKTHFSSLIKSTETTLQLR---QIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVS--LVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAM------P---------------------------------ERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2513
            +P+  +F+ L+K      +LR   +IH  +++   S      T L ++ +  + +  A  +F+    + L  +N ++ G ++N   + ++     M  + ++P  +T   VL +V+ L L  +G  +HG  ++SG  FDS V +S  LV+MY K   +  A Q+FD   ERN    ++ WN +I+   +  N ++AM +F+ M      P                                 +RN+   NS I+   K  +++ A  +F +++ + +VSW  ++ G +QNG    AL+ F +M    ++P+  T VS ++A A++        IH   + + L  N     ALVDMYAKCG I+ A  +F+   E+ + TW+ MI G+  HGF   A+  F++M    IKP+ V FL++++ACSHSG V+ GL  F  M+ +YSIE +M HY  +VDLLGRAGRL+EA  FI +MP+ P    +GA+  AC+ HKN+  AE  +E+L  L P   G +V L+N+Y A   WE V +VR+ M  Q + K PG S +E+  +VHSF +G   H  +++IY  LE+++   +E GY+P+T  VL  +E + KE  L +HSEKLA++F L+NT+ GTTI + KNLRVC DCH+  K  S ++ REI++RD++RFHHFK G CSCGDYW
Sbjct:  132 EPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSG--FDSLVNISTALVDMYAKCGSLETARQLFDGMLERN----VVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKENYSIELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEKAAERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGCSMVEIKNEVHSFFSGSTAHPDSKKIYAFLEKLICHIKEAGYVPDTNLVL-GVENDVKEQLLSTHSEKLAISFGLLNTTAGTTIHVRKNLRVCADCHNATKYISLVTGREIVVRDMQRFHHFKNGACSCGDYW 809          

HSP 2 Score: 170.629 bits (431), Expect = 2.193e-42
Identity = 104/364 (28.57%), Postives = 179/364 (49.18%), Query Frame = -3

Query: 1377 ALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNI--------------------------------GSWNSFIN-------GLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTA 2351
            A  +F     K   L++ +++G  + S    ++  F+ M    V P    F ++LK         +G  +HG ++KSG   D F    L  MY K  ++  A +VFD  PER+    ++ WN ++ G  + G    A+E+ ++M E N+                                  ++S +N          K G L  A QLFD M E++VVSW ++++   QN + ++A+ +F KML+ G++P D++++ AL ACA +G LE G  IH   VE GL  N +   +L+ MY KC  + +A+ +F + + + + +W+ MI G+A +G    A+  F +M    +KPD   +++++TA
Sbjct:   88 AARVFEPIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERD----LVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITA 447          

HSP 3 Score: 101.293 bits (251), Expect = 1.457e-20
Identity = 74/286 (25.87%), Postives = 135/286 (47.20%), Query Frame = -3

Query: 1023 KNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFD-SMR--FDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRML 1871
            + G +++A ++F+ +  K  V + T++ G ++  D  KAL  F +M    + P        L  C     L  G  IH   V++G  L+      L +MYAKC  +  A KVF+   E+D+ +W+ ++ G++ +G    A+   K M    +KP  +  +++L A S    + +G      +MR  FD  +  +    T +VD+  + G L+ A +    M +  + V+W ++  A   ++N K A L+ +K+L  E   P     +  ++A      D+ER R +
Sbjct:   81 RYGSVDEAARVFEPIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNIS----TALVDMYAKCGSLETARQLFDGM-LERNVVSWNSMIDAYVQNENPKEAMLIFQKMLD-EGVKPTDVSVMGALHAC-ADLGDLERGRFI 359          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PP258_ARATH (Pentatricopeptide repeat-containing protein At3g26782, mitochondrial OS=Arabidopsis thaliana GN=PCMP-H34 PE=2 SV=1)

HSP 1 Score: 432.18 bits (1110), Expect = 1.943e-137
Identity = 230/634 (36.28%), Postives = 366/634 (57.73%), Query Frame = -3

Query:  591 SIFNHSHHKS-LFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAM----------------------------P-----------------ERNIGSWNSFINGLMKNGD--LNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRP-NDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2345
            ++FN    K+ +F +N++I  L  +     ++  F  M +  + P + +FP  +K+ + L   F G   H +    G + D FV  +L+ MY    ++  A +VFDE P+RN    I+ W  +I G    GN   A+ LF+ +                            P                 +R +   N+ ++   K G+  +  A ++FD++ +KD VS+ +I++  +Q+G   +A  +F ++++  +   N +TL + L A +  GAL  G  IH+  +  GL  +     +++DMY KCG + +A K F+  K K++R+W+ MI G+ +HG   +A+  F  M+ SG++P+ + F+++L ACSH+G    G  +F++M+  + +EP ++HY  +VDLLGRAG L +A   IQRM + PD + W +L  ACR HKN+++AE+   +L  L+  + G Y+ LS++YA  GRW+DVERVRM+M+N+ + K PG+S +E+NG+VH F+ GD +H +  +IY  L E+     E GY+  T  V H+++EEEKE  L  HSEKLA+AF +MNT PG+T+ +VKNLRVC DCH+++K  SK+  RE ++RD KRFHHFK G CSCGDYW
Sbjct:   30 TLFNRYVDKTDVFSWNSVIADLARSGDSAEALLAFSSMRKLSLYPTRSSFPCAIKACSSLFDIFSGKQTHQQAFVFGYQSDIFVSSALIVMYSTCGKLEDARKVFDEIPKRN----IVSWTSMIRGYDLNGNALDAVSLFKDLLVDENDDDDAMFLDSMGLVSVISACSRVPAKGLTESIHSFVIKRGFDRGVSVGNTLLDAYAKGGEGGVAVARKIFDQIVDKDRVSYNSIMSVYAQSGMSNEAFEVFRRLVKNKVVTFNAITLSTVLLAVSHSGALRIGKCIHDQVIRMGLEDDVIVGTSIIDMYCKCGRVETARKAFDRMKNKNVRSWTAMIAGYGMHGHAAKALELFPAMIDSGVRPNYITFVSVLAACSHAGLHVEGWRWFNAMKGRFGVEPGLEHYGCMVDLLGRAGFLQKAYDLIQRMKMKPDSIIWSSLLAACRIHKNVELAEISVARLFELDSSNCGYYMLLSHIYADAGRWKDVERVRMIMKNRGLVKPPGFSLLELNGEVHVFLIGDEEHPQREKIYEFLAELNRKLLEAGYVSNTSSVCHDVDEEEKEMTLRVHSEKLAIAFGIMNTVPGSTVNVVKNLRVCSDCHNVIKLISKIVDREFVVRDAKRFHHFKDGGCSCGDYW 659          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PP320_ARATH (Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis thaliana GN=DOT4 PE=2 SV=1)

HSP 1 Score: 437.573 bits (1124), Expect = 6.641e-137
Identity = 241/660 (36.52%), Postives = 367/660 (55.61%), Query Frame = -3

Query:  591 QIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIG--------------------------SW-------------NSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVG-LRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2450
            Q+H  IL+       ++   L++     + +  A  +F+    + +  +N++I G   N   +  +S F+ ML   +  D  T   V    A   L  LG  +H   +K+    +     +L++MY K  ++  A  VF E  +R    S++ +  +I G  + G   +A++LFE M E  I                            W             N+ ++   K G + +A  +F EM+ KD++SW TI+ G S+N    +ALS+F  +LE     P++ T+   L ACA + A + G  IH Y + NG   ++  A +LVDMYAKCG +L A  +F++   KD+ +W+VMI G+ +HGF  +AI  F +M  +GI+ D + F++LL ACSHSG VD G  FF+ MR +  IEPT++HY  +VD+L R G L +A +FI+ MPI PD   WGAL C CR H ++K+AE V+EK+  LEP++ G YV ++N+YA   +WE V+R+R  +  + + K+PG S+IE+ G+V+ FVAGD  +     I   L ++ +   E+GY P T + L + EE EKE+AL  HSEKLA+A  ++++  G  IR+ KNLRVCGDCH + K  SK+++REI+LRD  RFH FK G CSC  +W
Sbjct:  216 QLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDR----SVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPDATIWGALLCGCRIHHDVKLAEKVAEKVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIEIKGRVNIFVAGDSSNPETENIEAFLRKVRARMIEEGYSPLTKYALIDAEEMEKEEALCGHSEKLAMALGIISSGHGKIIRVTKNLRVCGDCHEMAKFMSKLTRREIVLRDSNRFHQFKDGHCSCRGFW 871          

HSP 2 Score: 158.688 bits (400), Expect = 2.124e-38
Identity = 126/438 (28.77%), Postives = 201/438 (45.89%), Query Frame = -3

Query: 1161 ALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNI------------GSWNS-----------------------FINGLM----KNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSG-IKPDAVVFLALLTACSHSGQVDLGLNFFDS-MRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAH 2351
            A  +F+    +    +N L+  L ++  F  S+  F  M+   V  D  TF  V KS + L     G  LHG I+KSG    + V  SLV  Y+K + +  A +VFDE  ER+    ++ WN +ING    G  EK + +F  M    I            G  +S                       F N L+    K GDL+ A  +F EM ++ VVS+T+++ G ++ G   +A+ +F +M E G+ P+  T+ + L+ CA+   L+ G R+H +  EN L  +   + AL+DMYAKCG++  A  VF E + KDI +W+ +I G++ + +  +A+  F  ++      PD      +L AC+     D G       MR  Y  +  + +   +VD+  + G L  A      +  S D V+W  +      H
Sbjct:  148 ASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERD----VISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANS--LVDMYAKCGALLLAHMLFDDIA-SKDLVSWTVMIAGYGMH 578          

HSP 3 Score: 103.219 bits (256), Expect = 3.616e-21
Identity = 75/315 (23.81%), Postives = 147/315 (46.67%), Query Frame = -3

Query: 1032 GDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLG----------------------LNFF------DSMR--FDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMP---ISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSN----VYAAVGRWEDVERV 1865
            GDL +A ++FDE+K +  + W  ++N ++++GD   ++ +F KM+  G+  +  T      + + + ++  G ++H + +++G     +   +LV  Y K   + SA KVF+E  E+D+ +W+ +I G+  +G   + +  F +M+ SGI+ D    +++   C+ S  + LG                      L+ +      DS +  F    + ++  YT ++    R G   EA+K  + M    ISPD     A+   C  ++ +   + V E    ++    G  +F+SN    +YA  G  ++ E V
Sbjct:  143 GDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHE---WIKENDLGFDIFVSNALMDMYAKCGSMQEAELV 454          

HSP 4 Score: 67.0106 bits (162), Expect = 5.808e-10
Identity = 53/220 (24.09%), Postives = 98/220 (44.55%), Query Frame = -3

Query: 1179 EKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACS-----HSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALF 1823
            ++ V    T +    ++G+ + A+ +     +  + P   TL S L  CA   +L+ G  + N+   NG  ++    + L  MY  CG++  AS+VF+E K +    W++++   A  G +  +I  FKKMM SG++ D+  F  +  + S     H G+   G           S+  ++  + L      +  R+D A K    M    D ++W ++ 
Sbjct:   58 DRSVTDANTQLRRFCESGNLENAVKLLCVSGKWDIDPR--TLCSVLQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYL------KNQRVDSARKVFDEMT-ERDVISWNSII 268          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PP316_ARATH (Pentatricopeptide repeat-containing protein At4g16835, mitochondrial OS=Arabidopsis thaliana GN=DYW10 PE=2 SV=3)

HSP 1 Score: 428.713 bits (1101), Expect = 3.451e-136
Identity = 226/589 (38.37%), Postives = 349/589 (59.25%), Query Frame = -3

Query:  591 ALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKS-VAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMK-EKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2351
            AL +F+    K+   +N+L+ G++++    S +     +  +   PD  ++  +L   V  +              K    +++     ++  Y +  EM  A ++F    E+N+    + WN +I+G  + G+LEKA   F+  P R + +W + I G MK   +  A  +F +M   K++V+W  +++G  +N   +  L +F  MLE G+RPN   L SAL  C+++ AL+ G +IH    ++ L  +     +L+ MY KCG +  A K+FE  K+KD+  W+ MI G+A HG   +A+  F++M+ + I+PD + F+A+L AC+H+G V++G+ +F+SM  DY +EP   HYT +VDLLGRAG+L+EA+K I+ MP  P    +G L  ACR HKN+++AE  +EKLL+L  ++   YV L+N+YA+  RWEDV RVR  M+   V K PG+S+IE+  +VH F + D  H     I+ KL+E+    +  GY PE  + LHN+EEE+KE  L  HSEKLA+AF  +    G+ I++ KNLR+CGDCH  +K  S++ +REII+RD  RFHHFK G CSCGDYW
Sbjct:   80 ALRVFHGMRAKNTITWNSLLIGISKDP---SRMMEAHQLFDEIPEPDTFSYNIMLSCYVRNVNFEKAQSFFDRMPFKDAASWNT-----MITGYARRGEMEKARELFYSMMEKNE----VSWNAMISGYIECGDLEKASHFFKVAPVRGVVAWTAMITGYMKAKKVELAEAMFKDMTVNKNLVTWNAMISGYVENSRPEDGLKLFRAMLEEGIRPNSSGLSSALLGCSELSALQLGRQIHQIVSKSTLCNDVTALTSLISMYCKCGELGDAWKLFEVMKKKDVVAWNAMISGYAQHGNADKALCLFREMIDNKIRPDWITFVAVLLACNHAGLVNIGMAYFESMVRDYKVEPQPDHYTCMVDLLGRAGKLEEALKLIRSMPFRPHAAVFGTLLGACRVHKNVELAEFAAEKLLQLNSQNAAGYVQLANIYASKNRWEDVARVRKRMKESNVVKVPGYSWIEIRNKVHHFRSSDRIHPELDSIHKKLKELEKKMKLAGYKPELEFALHNVEEEQKEKLLLWHSEKLAVAFGCIKLPQGSQIQVFKNLRICGDCHKAIKFISEIEKREIIVRDTTRFHHFKDGSCSCGDYW 656          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy Swiss-Prot
Match: PPR53_ARATH (Pentatricopeptide repeat-containing protein At1g20230 OS=Arabidopsis thaliana GN=PCMP-H21 PE=2 SV=2)

HSP 1 Score: 430.639 bits (1106), Expect = 1.578e-135
Identity = 216/576 (37.50%), Postives = 341/576 (59.20%), Query Frame = -3

Query:  591 FNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEK----DVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2306
            +N ++ G   + + + +V  F  +      PD++T   VL SV    +  +G ++HG +IK G+  D  V  ++++MY K   +   + +F++                              E+ EA      G  N++I GL +NG ++KA+++F+  KE+    +VVSWT+I+ G +QNG   +AL +F +M   G++PN +T+ S L AC  I AL  G   H + V   L  N    +AL+DMYAKCG I  +  VF     K++  W+ ++ G+++HG   + +  F+ +M + +KPD + F +LL+AC   G  D G  +F  M  +Y I+P ++HY+ +V+LLGRAG+L EA   I+ MP  PD   WGAL  +CR   N+ +AE+ +EKL  LEP++PG+YV LSN+YAA G W +V+ +R  M++  + K+PG S+I+V  +V++ +AGD  H +  +I  K++EI    R+ G+ P   + LH++EE+E+E  L  HSEKLA+ F L+NT  GT ++++KNLR+CGDCH+++K  S  + REI +RD  RFHHFK G+CSCGD+W
Sbjct:  220 WNGILSGFNRSGYHKEAVVMFQKIHHLGFCPDQVTVSSVLPSVGDSEMLNMGRLIHGYVIKQGLLKDKCVISAMIDMYGKSGHVYGIISLFNQ-----------------------------FEMMEA------GVCNAYITGLSRNGLVDKALEMFELFKEQTMELNVVSWTSIIAGCAQNGKDIEALELFREMQVAGVKPNHVTIPSMLPACGNIAALGHGRSTHGFAVRVHLLDNVHVGSALIDMYAKCGRINLSQIVFNMMPTKNLVCWNSLMNGFSMHGKAKEVMSIFESLMRTRLKPDFISFTSLLSACGQVGLTDEGWKYFKMMSEEYGIKPRLEHYSCMVNLLGRAGKLQEAYDLIKEMPFEPDSCVWGALLNSCRLQNNVDLAEIAAEKLFHLEPENPGTYVLLSNIYAAKGMWTEVDSIRNKMESLGLKKNPGCSWIQVKNRVYTLLAGDKSHPQIDQITEKMDEISKEMRKSGHRPNLDFALHDVEEQEQEQMLWGHSEKLAVVFGLLNTPDGTPLQVIKNLRICGDCHAVIKFISSYAGREIFIRDTNRFHHFKDGICSCGDFW 760          

HSP 2 Score: 139.428 bits (350), Expect = 1.392e-32
Identity = 129/570 (22.63%), Postives = 241/570 (42.28%), Query Frame = -3

Query:  738 SLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVF-----------------------------------EETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVG-KDPGWSYIEVNGQVHSFVAGDHDHKRAREIYL----KLEEIVSGAREQGYMPET---GWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTI 2318
            +++ F++LI  LT+   F  S+  F  M    + PD    P + K  A L    +G  +H     SG++ D+FV+ S+  MY++   MG A +VFD    R   + ++  + L+    + G LE+ + +   M    I                           E ++VSW  I++G +++G H++A+ MF K+  +G  P+ +T+ S L +      L  G  IH Y ++ GL  +K   +A++DMY K G++     +F                                   E+T E ++ +W+ +I G A +G   +A+  F++M  +G+KP+ V   ++L AC +   +  G +          +   +   + ++D+  + GR++ +      MP + + V W +L      H   K    + E L+R   K    ++  +++ +A G+               VG  D GW Y ++  + +  +    +H       L    KL+E     +E  + P++   G +L++   +   D     +EKL   F L   +PGT +
Sbjct:   80 TIYSFSSLIYALTKAKLFTQSIGVFSRMFSHGLIPDSHVLPNLFKVCAELSAFKVGKQIHCVSCVSGLDMDAFVQGSMFHMYMRCGRMGDARKVFD----RMSDKDVVTCSALLCAYARKGCLEEVVRILSEMESSGI---------------------------EANIVSWNGILSGFNRSGYHKEAVVMFQKIHHLGFCPDQVTVSSVLPSVGDSEMLNMGRLIHGYVIKQGLLKDKCVISAMIDMYGKSGHVYGIISLFNQFEMMEAGVCNAYITGLSRNGLVDKALEMFELFKEQTMELNVVSWTSIIAGCAQNGKDIEALELFREMQVAGVKPNHVTIPSMLPACGNIAALGHGRS-THGFAVRVHLLDNVHVGSALIDMYAKCGRINLSQIVFNMMP-TKNLVCWNSLMNGFSMHGKAKEVMSIFESLMRTRLKP--DFISFTSLLSACGQ---------------VGLTDEGWKYFKMMSEEYG-IKPRLEHYSCMVNLLGRAGKLQEAYDLIKEMPFEPDSCVWGALLNSCRLQNNVDLAEIAAEKL---FHLEPENPGTYV 595          

HSP 3 Score: 76.6406 bits (187), Expect = 5.644e-13
Identity = 40/166 (24.10%), Postives = 85/166 (51.20%), Query Frame = -3

Query: 1362 LNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSG 1859
             N A  +   + +  + S+++++  +++     +++ +F +M   GL P+   L +    CA++ A + G +IH     +GL ++     ++  MY +CG +  A KVF+   +KD+ T S ++  +A  G   + +R   +M  SGI+ + V +  +L+  + SG
Sbjct:   66 FNDADLVLQSIPDPTIYSFSSLIYALTKAKLFTQSIGVFSRMFSHGLIPDSHVLPNLFKVCAELSAFKVGKQIHCVSCVSGLDMDAFVQGSMFHMYMRCGRMGDARKVFDRMSDKDVVTCSALLCAYARKGCLEEVVRILSEMESSGIEANIVSWNGILSGFNRSG 231          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: A0A078DLL1_BRANA (BnaA10g03000D protein OS=Brassica napus GN=BnaA10g03000D PE=4 SV=1)

HSP 1 Score: 779.245 bits (2011), Expect = 0.000e+0
Identity = 378/641 (58.97%), Postives = 494/641 (77.07%), Query Frame = -3

Query:  591 PLKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAV-GRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2510
            P ++HF SLI S + T+ LR+++AQILRR   +S  + + L+S SS LKS  Y LSIF +S  K+LF FNALIRGLTEN+ F+ S+ HF+LMLR  VRPD+LTFPF LKS + LG R+LG  LH   +K  V+ DSFVRVSLV+MY K + + +A QVFDESPER K ES+LLWNVLING C+  +L+ A  LF +MPERN GSW++ I G +  G+LN+A Q F+ M EK+VVSWTT++NG SQNGD++ A+S++F+M+E G++PN+ T+ + LSAC+K GALE+G+R+H Y ++NG +L++A   ALVDMYAKCG +  A+ VF    +KDI +W+ MI GWAIHG + QAI CF++MM+SG KPD VVFLA+LTAC +SG+VDLG+NFFDSMR DY IEPT+KHY +VVDLLGRAG+L+EA + I+ MPI+PD   W AL+ AC+AH + +  E+VS+ L+ ++P+  GSY+FL   YAA  G+  DVE+ R +++ +  GK  GWSYIEV+GQ++ FVAGD+ HK+A+EI LKLEEIVS A E+GY+P   W +H+IEEEEKE+  G HSEKLALA  L+ TSPGT IRIVKNLR+CGDCHSLMK  SK+SQR+I+LRD ++FHHFK G CSCGDYW
Sbjct:   28 PDESHFISLIHSCKDTVSLRRVYAQILRRSFLTS-RVASQLLSCSSLLKSPDYTLSIFRYSKEKNLFTFNALIRGLTENARFECSIRHFILMLRLGVRPDRLTFPFALKSNSKLGFRWLGKALHAATVKDCVDCDSFVRVSLVDMYAKTKGLKYAYQVFDESPERIKKESVLLWNVLINGYCRAKDLQTAATLFGSMPERNSGSWSTLIKGYVDIGELNRAKQCFELMLEKNVVSWTTLINGFSQNGDYETAISIYFEMVEKGMKPNEYTVAAVLSACSKSGALESGIRVHGYVLDNGFKLDRAIGTALVDMYAKCGEVDCAANVFSNMGDKDILSWTAMIQGWAIHGRFQQAILCFREMMYSGEKPDEVVFLAVLTACLNSGEVDLGINFFDSMRLDYMIEPTLKHYLVVVDLLGRAGKLNEAHELIESMPINPDLTTWAALYRACKAHNSNRKGEMVSQNLVEIDPELRGSYIFLDKRYAATKGKHRDVEKRRKIVKER--GK--GWSYIEVDGQLNKFVAGDNSHKQAQEIRLKLEEIVSLAVERGYIPGGDWSIHDIEEEEKENVTGIHSEKLALALGLLRTSPGTVIRIVKNLRICGDCHSLMKYVSKISQRDILLRDARQFHHFKEGSCSCGDYW 663          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: V4L9I4_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10007007mg PE=4 SV=1)

HSP 1 Score: 767.303 bits (1980), Expect = 0.000e+0
Identity = 364/640 (56.88%), Postives = 488/640 (76.25%), Query Frame = -3

Query:  591 PLKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2510
            P ++H  SLI + + T+ LR++HA ILRR + SS  +   L+S SS LKS  Y+LSIF +   K+LF+FNALIRGL E++ F+ SV HF+LMLR  VRPD+LTFPFVLKS + LG R+LG  LH   +K  V+ DSFVRVSLV+MY K   + +A QVFDESP+  K E ILLWNVLING C+  +++ A  LF +MPERN GSW++ I G + NGDLN+A QLF+ M EK VVSWTT++NG SQNGD++ A+S +F+MLE G++PN+ T+ + LSAC+K GAL +G+RIH Y ++NG+ L++A   AL+DMYAKCG +  A+ VF   + KDI +W+ MI GWA+HG + ++I CF++M+FSG KPD VVFLA+LTAC ++G+VDLG+NFFDSMR DY+IEPT+KHY LVVD+LGRAG+L++A + I+ MPI+PD   W AL+ AC+AHK+ +  E+VS+ LL L+P+  GSY+FL   Y A G+++DVE+ R+ +Q     +  GWSYIE++GQ++ F AGD+ HK+A+EI LKLEEI+S A E+GY+    W +H+IEEEEKE+  G HSEKLALA  ++ T+PGT IRI+KNLR+CGDCHSLMK  SK+SQR+++LRD ++FHHFK G CSCGDYW
Sbjct:   27 PDESHIISLIHACKDTVCLRRVHAYILRRGVLSS-RVAAQLVSSSSLLKSPDYSLSIFRYLKEKNLFVFNALIRGLAESARFKCSVRHFILMLRLGVRPDRLTFPFVLKSNSKLGFRWLGRALHAAALKDSVDCDSFVRVSLVDMYAKTGGLKYAFQVFDESPDWIKMERILLWNVLINGYCRAKDMQMATTLFGSMPERNSGSWSTLIKGYVDNGDLNRARQLFEVMPEKSVVSWTTLINGFSQNGDYESAISTYFEMLEEGMKPNEYTVAAVLSACSKSGALGSGIRIHGYILDNGINLDRAIGTALIDMYAKCGEVDCAATVFSNMRHKDILSWTAMIQGWALHGRFQESILCFRQMLFSGEKPDEVVFLAVLTACLNAGEVDLGINFFDSMRLDYAIEPTLKHYVLVVDMLGRAGKLNQAHELIENMPINPDLTTWAALYRACKAHKSNRKGEMVSQNLLELDPELRGSYIFLGKTYEAKGKYQDVEKKRLPLQKIVKERCKGWSYIELDGQLNKFAAGDNSHKQAQEIRLKLEEIISLAIERGYITGADWSIHDIEEEEKENVTGIHSEKLALALGILRTAPGTAIRIIKNLRICGDCHSLMKYVSKISQRDLLLRDARQFHHFKEGSCSCGDYW 665          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: A0A067DYD8_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g006672mg PE=4 SV=1)

HSP 1 Score: 764.992 bits (1974), Expect = 0.000e+0
Identity = 398/638 (62.38%), Postives = 483/638 (75.71%), Query Frame = -3

Query:  591 KTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2504
            +TH  SLI S+ +T QLRQIHAQI+  +L +S+ +TT LIS +S  KSI YALSIF+H   K+L +FN LIRGL ENSHFQS +SHF+ MLR  VRP++LT+PFV KSVA L L  LG  LH  I+KSGVE+D+FVRV L +MYV+L +   A +VFDE+PE+NKSES+LLWNVLINGC K+G L KA+ELF  MP++N+ SW S I+G M+ GDL KA +LF++M EK VVSWT ++NG SQNG+ +KAL+MFF+ML+ G+R ND T+VSALSACAK+GALEAGVR+HNY   N   L  A   ALVDMYAKCGNI +AS VF ETKEKD+ TW+ MIWG AIHG Y QAI+ FKKMM+SG +PD  VFLA+LTAC +SGQV L LNFFDSMRFDY IEP++KH+T+VV+LL R G+  +  +  Q       F                        KLL+L+PKHP SYV LSN+YAA GRW+DV RVR LMQ +++ KDPGWSYIEVNG VH F AG   HK A+EI+ KLE+I++GAREQGYMP T WVLHNI +EEKE+ALG HSEKLALAF L+ T+PGTTI+IVK L +CGDCHSLMK ASK+SQREI+LRD  RFH+FK G CSC DYW
Sbjct:   31 ETHIISLIHSSNSTKQLRQIHAQIILHNLFASSRITTQLISSASLHKSIDYALSIFDHFTPKNLHIFNVLIRGLAENSHFQSCISHFVFMLRLSVRPNRLTYPFVSKSVASLSLLSLGRGLHCLIVKSGVEYDAFVRVHLADMYVQLGKTRGAFKVFDETPEKNKSESVLLWNVLINGCSKIGYLRKAVELFGMMPKKNVASWVSLIDGFMRKGDLKKAGELFEQMPEKGVVSWTAMINGFSQNGEAEKALAMFFQMLDAGVRANDFTVVSALSACAKVGALEAGVRVHNYISCNDFGLKGAIGTALVDMYAKCGNIEAASLVFGETKEKDLLTWTAMIWGLAIHGRYEQAIQYFKKMMYSGTEPDGTVFLAILTACWYSGQVKLALNFFDSMRFDYFIEPSVKHHTVVVNLLSRVGQDSQGYQNSQN-----SFT-----------------------KLLQLKPKHPSSYVLLSNIYAAEGRWKDVARVRTLMQRRSIKKDPGWSYIEVNGHVHRFEAG--GHKLAKEIHSKLEDIMAGAREQGYMPGTEWVLHNI-KEEKEEALGCHSEKLALAFGLIQTTPGTTIKIVKKLTICGDCHSLMKYASKISQREIVLRDT-RFHYFKDGTCSCRDYW 636          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: D7KE83_ARALL (Pentatricopeptide repeat-containing protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_311569 PE=4 SV=1)

HSP 1 Score: 762.681 bits (1968), Expect = 0.000e+0
Identity = 364/640 (56.88%), Postives = 488/640 (76.25%), Query Frame = -3

Query:  591 PLKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2510
            P ++HF SLI + + T+ LR +HA ILRR + SS  +   L+S SS LKS  Y+LSIF +S  ++ F+FNALIRGLTEN+ F+ SV HF+LML   V+PD+LTFPFVLKS + LG R+LG  LH   +K+ V+ DSFVRVSLV+MY K  ++  A QVF+E+P+R K ESILLWNVL+NG C+  +++ A  LF +MPERN GSW++ I G + NG+LN+A QLF+ M EK+VVSWTT++NG SQ GD++ A+S +F+MLE GL+PN+ T+ + LSAC+K GAL +G+RIH Y ++NG++L++A   +L+DMYAKCG +  A+ VF     KDI +W+ MI GWA+HG + QAI+CF++MM+SG KPD VVFLA+LTAC +SG+VDLGLNFFDSMR DY+IEPT+KHY LVVDLLGRAG+LDEA + ++ MPI+PD   W AL+ AC+AHK+ +  ++V + LL L+P+  GSY+FL   +AA G+++DVE+ R+ +Q +   +  G SYIE++ Q++ F A D+ HK+A+EI LKLE I+S A E+GY+P   W +H+IEEEEKE   G HSEKLAL   L+ T+PGTTIRI+KNLR+CGDCHSLMK  SK+SQR I+LRD ++FHHFK G CSCGDYW
Sbjct:   27 PDESHFISLIHTCKDTVSLRLVHAHILRRGVLSS-RVAAQLVSCSSLLKSPDYSLSIFRNSEERNPFVFNALIRGLTENARFECSVRHFILMLTLGVKPDRLTFPFVLKSNSKLGFRWLGRALHAATLKNFVDCDSFVRVSLVDMYAKTGQLNHAFQVFEETPDRIKKESILLWNVLVNGYCRAKDMQMATTLFRSMPERNSGSWSTLIKGYVDNGELNRAKQLFELMPEKNVVSWTTLINGFSQTGDYETAISTYFEMLEKGLKPNEYTVAAVLSACSKSGALGSGIRIHGYILDNGIKLDRAIGTSLLDMYAKCGEVDCAATVFSNMNHKDILSWTAMIQGWAVHGRFHQAIQCFRQMMYSGEKPDEVVFLAVLTACLNSGEVDLGLNFFDSMRLDYAIEPTLKHYVLVVDLLGRAGKLDEAHELVEYMPINPDLTTWAALYRACKAHKSNR-TDIVLQNLLELDPELRGSYIFLDKTHAAKGKYQDVEKRRLSLQKKVKERSMGCSYIELDCQLNKFAADDYTHKQAQEIRLKLEGIISLAIERGYIPGADWSIHDIEEEEKESVTGIHSEKLALTLGLLRTAPGTTIRIIKNLRICGDCHSLMKYVSKISQRGILLRDARQFHHFKDGSCSCGDYW 664          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: R0GVK3_9BRAS (Uncharacterized protein (Fragment) OS=Capsella rubella GN=CARUB_v10008505mg PE=4 SV=1)

HSP 1 Score: 762.681 bits (1968), Expect = 0.000e+0
Identity = 367/640 (57.34%), Postives = 478/640 (74.69%), Query Frame = -3

Query:  591 PLKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2510
            P ++HF SLI + + T+ LR++HAQILRR + SS  +   L+S S  L+S  Y LSIF +   K+LF+FN LIRGLTEN+   SSV HF+LMLR  VRPD+LTFPFVLKS + LG R+LG  LH   +K+ V+ DSFVRVSLV+MY K  ++ +A QVFDESP+R K ES LL NVLI G C+  +++ A +LF +MPERN GSW++ I G      LN+A QLF+ M EK VV+WTT++NG SQNG ++ A+S +F+MLE GL+PN+ T+ +ALSAC+K GAL +G+RIH Y ++NG+RL++A   AL+DMYAKCG +  A  VF     KDI +W+ MI GWA+HG + QAI+CF++MM+SG KPD VVFLA+LTAC +SG+VDLGLNFFDSMR DY+IEPT+KHY LVVDLLGRAG+L+EA KF+  MPI+PDF  W AL+ A +AH++ +  E+VS+ LL L+P+  GSY+FL   + A G+++DVE  R+  Q     +  GWSYIE++ Q++ F AGD  HK+ +EI LKLEEI+S A E GY+P   W +H+IEEEEKE   G HSEKLAL   ++ T+PGTTIRI+KNLR+CGDCHSLMK  SK+SQR I+LRD ++FHHFK G CSCGDYW
Sbjct:   34 PDESHFISLIHACKDTVSLRRVHAQILRRGVLSS-RVAAQLVSCSGLLQSPDYCLSIFRNFEEKNLFVFNVLIRGLTENARSASSVRHFILMLRLGVRPDRLTFPFVLKSNSKLGFRWLGRALHAATLKNFVDCDSFVRVSLVDMYAKTRQLNYAFQVFDESPDRIKKESTLLSNVLIKGYCRAKDMQMATKLFRSMPERNSGSWSTLIKGYADCSQLNRAKQLFELMPEKHVVTWTTLINGFSQNGYYETAISTYFEMLEKGLKPNEYTVAAALSACSKSGALGSGIRIHAYILDNGIRLDRAIGTALIDMYAKCGEVDCAGTVFSNMNHKDILSWTAMIQGWAVHGCFHQAIQCFRQMMYSGEKPDEVVFLAVLTACLNSGEVDLGLNFFDSMRLDYAIEPTLKHYVLVVDLLGRAGKLNEAHKFVDNMPINPDFTTWAALYRASKAHRSNRRTEIVSQNLLELDPELRGSYIFLDKTHLANGKYQDVEERRVPYQKTGKERSLGWSYIELDCQLNKFAAGDCTHKQTQEIRLKLEEIISLATEIGYIPGADWSIHDIEEEEKEHVTGVHSEKLALTLGILRTAPGTTIRIIKNLRICGDCHSLMKYVSKISQRGILLRDARQFHHFKDGSCSCGDYW 672          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: A0A0D3C7M6_BRAOL (Uncharacterized protein OS=Brassica oleracea var. oleracea PE=4 SV=1)

HSP 1 Score: 760.755 bits (1963), Expect = 0.000e+0
Identity = 365/640 (57.03%), Postives = 487/640 (76.09%), Query Frame = -3

Query:  591 PLKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2510
            P ++HF  LI S +    LR+++AQILRR L +S  + + L+S SS LKS  Y LSIF +S  K+LF+FNALIRGLTEN+ F+ S+ HF++MLR  VRPD+LTFPFVLKS + LG R+LG  LH   +K  V+ DSFVRVSLV+MY K + + +A QVFDESPER K E +LLWNVLING C+  +++ A  LF +MPERN GSW++ I G + +G+LN+A Q F+ M EK+VVS TT++NG SQN +++ A+S++F+M+E G++PN+ T+ + LSAC+K GALE+G+R+H Y ++NG +L++A   ALVDMYAKCG +  A+ VF    +KDI +W+ MI GWA+HG + QAI CF++MM+SG KPD VVFLA+LTAC +SG+VDLG+NFFDSMR DY+IEPT+KHY +VVDLLGRAG+L+EA + I+ MPI PD   W AL+ AC+AH + +  E+VS+ L+ ++ +  GSY+FL+    A G+ +DVE+ R+ +Q     +  GWSYIEV+GQ++ FVAGD  HK+A+EI LKLEEIVS A E+GY+P   W +H+IEEEEKE+  G HSEKLALA  L+ T+PGT IRIVKNLR+CGDCHSLMK  S++ QREI+LRD ++FHHFK G CSCGDYW
Sbjct:   28 PDESHFIPLIHSCKDIASLRRVYAQILRRSLLTS-RVASQLLSCSSLLKSPDYTLSIFRYSKEKNLFVFNALIRGLTENARFECSLRHFIMMLRLGVRPDRLTFPFVLKSNSKLGFRWLGKALHAATVKDCVDCDSFVRVSLVDMYGKTKGLKYAYQVFDESPERIKKEGVLLWNVLINGYCRAKDMQTAATLFGSMPERNSGSWSTLIKGYVDSGELNRAKQCFELMPEKNVVSLTTLINGFSQNDEYETAISIYFEMVEKGMKPNEYTVAAVLSACSKSGALESGIRVHGYVLDNGFKLDRAIGTALVDMYAKCGEVDCAANVFSNMGDKDILSWTAMIQGWAVHGRFQQAILCFREMMYSGEKPDEVVFLAVLTACLNSGEVDLGINFFDSMRLDYAIEPTLKHYVVVVDLLGRAGKLNEAHELIESMPIKPDLTTWAALYRACKAHNSNRKGEMVSQNLVEIDTELRGSYIFLAE---AKGKHQDVEKRRLPLQKMVKERCKGWSYIEVDGQLNKFVAGDDSHKQAQEIRLKLEEIVSLAVERGYIPGGDWSIHDIEEEEKENVTGIHSEKLALALGLLRTAPGTVIRIVKNLRICGDCHSLMKYVSRIKQREILLRDARQFHHFKEGSCSCGDYW 663          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: M4DFT4_BRARP (Uncharacterized protein OS=Brassica rapa subsp. pekinensis GN=Bra015357 PE=4 SV=1)

HSP 1 Score: 723.005 bits (1865), Expect = 0.000e+0
Identity = 354/610 (58.03%), Postives = 464/610 (76.07%), Query Frame = -3

Query:  687 PLKTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVG-LRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAV-GRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMK 2510
            P ++HF SLI S + T+ LR+++AQILRR   +S  + + L+S SS LKS  Y LSIF +S  K+LF FNALIRGLTEN+ F+ S+ HF+LMLR  VRPD+LTFPF LKS + LG R+LG  LH   +K  V+ DSFVRVSLV+MY K + + +A QVFDESPER K ES+LLWNVLING C+  +L+ A  LF +MPERN GSW++ I G +  G+LN+A Q F+ M EK+V+SWTT++NG SQNG+++ A+S++F M+E G ++PN+ T+ + LSAC+K GALE+G+R+H Y ++NG +L++A   ALVDMYAKCG +  A+ VF     KDI +W+ MI GWAIHG + QAI CF++MM+SG KPD VVFLA+LTAC +SG+VDLG+NFFDSMR DY IEPT+KHY +VVDLLGRAG+L+EA + I+ MPI+PD   W AL+ AC+AH + +  E+VS+ L+ ++P+  GSY+FL   YAA  G+  DVE+ R +++ +  GK  GWSYIEV+GQ++ FVAGD+ HK+A+EI LKLEEIVS A E GY+P   W +H+IEEEEKE+  G HSEKLALA  L+ TSPGT IRIVKNLR+CGDCHSLMK
Sbjct:  442 PDESHFISLIHSCKDTVSLRRVYAQILRRSFLTS-RVASQLLSCSSLLKSPDYTLSIFRYSKEKNLFTFNALIRGLTENARFECSIRHFILMLRLGVRPDRLTFPFALKSNSKLGFRWLGKALHAATVKDCVDCDSFVRVSLVDMYAKTKGLKYAYQVFDESPERIKKESVLLWNVLINGYCRAKDLQTAETLFGSMPERNSGSWSTLIKGYVDIGELNRAKQCFELMPEKNVLSWTTLINGFSQNGEYETAISIYFNMVEKGCMKPNEYTVAAVLSACSKSGALESGIRVHGYVLDNGFKLDRAIGTALVDMYAKCGEVDCAANVFSNMGVKDILSWTAMIQGWAIHGRFQQAILCFREMMYSGEKPDEVVFLAVLTACLNSGEVDLGINFFDSMRLDYMIEPTLKHYVVVVDLLGRAGKLNEAHELIESMPINPDLTTWAALYRACKAHNSNRKGEMVSQNLVEIDPELRGSYIFLDKRYAATKGKHRDVEKRRKIVKER--GK--GWSYIEVDGQLNKFVAGDNSHKQAQEIRLKLEEIVSLAVESGYLPGGDWSIHDIEEEEKENVTGIHSEKLALALGLLRTSPGTVIRIVKNLRICGDCHSLMK 1046          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: M0TR71_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1)

HSP 1 Score: 596.275 bits (1536), Expect = 0.000e+0
Identity = 313/638 (49.06%), Postives = 414/638 (64.89%), Query Frame = -3

Query:  591 HFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXX--XXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 2498
            H  S+I S+++  QL+Q+H ++LR  L  +A L   L+S + S  L+++PYAL++F HS   S  +F+AL+R L   S F +++SHF+L+ R  +RP +L FPF LKS A L        LH    KSG++ D FVR S+V+MYVKL   GFA             E  L                  M LFE+MP R++ SWNS I G M+ GD   A++LF +M +K+VVSWTT+V GV                     +PN+ T+ SALSACA++GALE G+RIH++ +++G R N     ALV MY+KCG I  A++VF+    +D+ TW+ MI GWAIHG + +A+RCF++M    I+PD  VFLA+L ACSHSG+V+ GL  FDSMRF+Y IEPT+KHYT +VDL GRAGRL++AM  ++ MP+ PDFV WGA F ACRA+KN+++AE+ +EKLL   PKH                     +VR +M++  + + PGWSYIEV G  H FVAGD  H R++EIY KLEE+ + A+EQGY P+T WVLHNIE+E+KE++LG HSEKLALA  L++T  G  IRIVKN RVCGDCH LMK ASK+  + IILRDIKRFHHFK G CSCGDYW
Sbjct:   19 HLISIIHSSKSAFQLKQVHGRVLRLRLPLTARLAAQLVSAACSPHLRAVPYALALFRHSP-PSPPVFDALVRALASCSLFSAALSHFVLITRSGLRPSRLAFPFALKSAAALPSPRHAACLHAAAAKSGLDLDLFVRTSIVDMYVKL---GFA-------------ECAL------------------MVLFESMPVRSVASWNSLIQGYMRQGDAQSAVELFHQMTQKNVVSWTTMVAGV---------------------QPNEFTISSALSACARMGALERGIRIHDFALKSGFRENGVIGTALVAMYSKCGKIELAAQVFDVMDRRDLLTWTSMILGWAIHGHWAKALRCFEEMKCECIEPDEAVFLAMLMACSHSGKVERGLQIFDSMRFEYKIEPTIKHYTCMVDLFGRAGRLNDAMSLMKAMPMEPDFVLWGAFFNACRANKNVELAEVAAEKLLNFRPKH---------------------QVRTVMKDSGIERLPGWSYIEVEGGGHRFVAGDRSHPRSKEIYGKLEELATRAKEQGYEPDTDWVLHNIEDEDKEESLGCHSEKLALALGLLSTPIGADIRIVKNRRVCGDCHLLMKFASKLCNKVIILRDIKRFHHFKDGRCSCGDYW 579          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: A0A078G0W6_BRANA (BnaC05g02860D protein OS=Brassica napus GN=BnaC05g02860D PE=4 SV=1)

HSP 1 Score: 575.859 bits (1483), Expect = 0.000e+0
Identity = 260/454 (57.27%), Postives = 352/454 (77.53%), Query Frame = -3

Query:  591 LEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHPGSYVFLSNVYAAVGRWEDVERVRMLMQNQAVGKDPGWSYIEVNGQVHSFVAGDHDHKRAREIYLKLEEIVSGAREQGYMPETGWVLHNIEEEEKEDALGSHSEKLALAFALMNTSPGTTIRIVKNLRVCGDCHSLMKCASKMSQREIILRDIKRFHHFKYGVCSCGDYW 1952
            ++ A  LF +MPERN GSW++ I G + +G+LN+A Q F+ M EK+VVS TT++NG SQN +++ A+S++F+M+E G++PN+ T+ + LSAC+K GALE+G+R+H Y ++NG +L++A   ALVDMYAKCG +  A+ VF    +KDI +W+ MI GWA+HG + QAI CF++MM+SG KPD VVFLA+LTAC +SG+VDLG+NFFDSMR DY+IEPT+KHY +VVDLLGRAG+L+EA + I+ MPI PD   W AL+ AC+AH + +  E+VS+ L+ ++P+  GSY+FL+    A G+ +DVE+ R+ +Q     +  GWSYIEV+GQ++ FVAGD  HK+A+EI LKLEEIVS A E+GY+P   W +H+IEEEEKE+  G HSEKLALA  L+ T+PGT IRIVKNLR+CGDCHSLMK  S++ QREI+LRD ++FHHFK G CSCGDYW
Sbjct:    1 MQTAATLFGSMPERNSGSWSTLIKGYVNSGELNRAKQCFELMPEKNVVSLTTLINGFSQNDEYETAISIYFEMVEKGMKPNEYTVAAVLSACSKSGALESGIRVHGYVLDNGFKLDRAIGTALVDMYAKCGEVDCAANVFSNMGDKDILSWTAMIQGWAVHGRFQQAILCFREMMYSGEKPDEVVFLAVLTACLNSGEVDLGINFFDSMRLDYAIEPTLKHYVVVVDLLGRAGKLNEAHELIESMPIKPDLTTWAALYRACKAHNSNRKGEMVSQNLVEIDPELRGSYIFLAE---AKGKHQDVEKRRLPLQKMVKERCKGWSYIEVDGQLNKFVAGDDSHKQAQEIRLKLEEIVSLAVERGYIPGGDWSIHDIEEEEKENVTGIHSEKLALALGLLRTAPGTVIRIVKNLRICGDCHSLMKYVSRIKQREILLRDARQFHHFKEGSCSCGDYW 451          

HSP 2 Score: 74.3294 bits (181), Expect = 1.517e-10
Identity = 54/254 (21.26%), Postives = 115/254 (45.28%), Query Frame = -3

Query: 1623 ALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAM------PERNIGSWNSFINGLMKNGDLNKAMQLFDEMK-----EKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVE 2351
            A   F     K++     LI G ++N  +++++S +  M+ + ++P++ T   VL + +  G    G+ +HG ++ +G + D  +  +LV+MY K  E+  A  VF    +++    IL W  +I G    G  ++A+  F  M      P+  +  + + +   + +G+++  +  FD M+     E  +  +  +V+ + + G   +A  +   M    ++P+  T  +   AC    +   G  +    VE
Sbjct:   35 AKQCFELMPEKNVVSLTTLINGFSQNDEYETAISIYFEMVEKGMKPNEYTVAAVLSACSKSGALESGIRVHGYVLDNGFKLDRAIGTALVDMYAKCGEVDCAANVFSNMGDKD----ILSWTAMIQGWAVHGRFQQAILCFREMMYSGEKPDEVV--FLAVLTACLNSGEVDLGINFFDSMRLDYAIEPTLKHYVVVVDLLGRAGKLNEAHELIESM---PIKPDLTTWAALYRACKAHNSNRKGEMVSQNLVE 279          

BLAST of g.barbadense_cottongen_reftransV1_0097138 vs. ExPASy TrEMBL
Match: A0A067DXZ0_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g006672mg PE=4 SV=1)

HSP 1 Score: 572.007 bits (1473), Expect = 0.000e+0
Identity = 292/469 (62.26%), Postives = 364/469 (77.61%), Query Frame = -3

Query: 1098 KTHFSSLIKSTETTLQLRQIHAQILRRHLSSSANXXXXXXXXXXXXXXIPYALSIFNHSHHKSLFLFNALIRGLTENSHFQSSVSHFLLMLRQRVRPDKLTFPFVLKSVAGLGLRFLGLILHGRIIKSGVEFDSFVRVSLVEMYVKLEEMGFALQVFDESPERNKSESILLWNVLINGCCKVGNLEKAMELFEAMPERNIGSWNSFINGLMKNGDLNKAMQLFDEMKEKDVVSWTTIVNGVSQNGDHQKALSMFFKMLEVGLRPNDLTLVSALSACAKIGALEAGVRIHNYFVENGLRLNKATAAALVDMYAKCGNILSASKVFEETKEKDIRTWSVMIWGWAIHGFYGQAIRCFKKMMFSGIKPDAVVFLALLTACSHSGQVDLGLNFFDSMRFDYSIEPTMKHYTLVVDLLGRAGRLDEAMKFIQRMPISPDFVAWGALFCACRAHKNIKMAELVSEKLLRLEPKHP 2504
            +TH  SLI S+ +T QLRQIHAQI+  +L +S+ +TT LIS +S  KSI YALSIF+H   K+L +FN LIRGL ENSHFQS +SHF+ MLR  VRP++LT+PFV KSVA L L  LG  LH  I+KSGVE+D+FVRV L +MYV+L +   A +VFDE+PE+NKSES+LLWNVLINGC K+G L KA+ELF  MP++N+ SW S I+G M+ GDL KA +LF++M EK VVSWT ++NG SQNG+ +KAL+MFF+ML+ G+R ND T+VSALSACAK+GALEAGVR+HNY   N   L  A   ALVDMYAKCGNI +AS VF ETKEKD+ TW+ MIWG AIHG Y QAI+ FKKMM+SG +PD  VFLA+LTAC +SGQV L LNFFDSMRFDY IEP++KH+T+VV+LL R G++D+A+ FI +MP +PDFV WGALFCACR HK+ K+A++  +    L    P
Sbjct:   31 ETHIISLIHSSNSTKQLRQIHAQIILHNLFASSRITTQLISSASLHKSIDYALSIFDHFTPKNLHIFNVLIRGLAENSHFQSCISHFVFMLRLSVRPNRLTYPFVSKSVASLSLLSLGRGLHCLIVKSGVEYDAFVRVHLADMYVQLGKTRGAFKVFDETPEKNKSESVLLWNVLINGCSKIGYLRKAVELFGMMPKKNVASWVSLIDGFMRKGDLKKAGELFEQMPEKGVVSWTAMINGFSQNGEAEKALAMFFQMLDAGVRANDFTVVSALSACAKVGALEAGVRVHNYISCNDFGLKGAIGTALVDMYAKCGNIEAASLVFGETKEKDLLTWTAMIWGLAIHGRYEQAIQYFKKMMYSGTEPDGTVFLAILTACWYSGQVKLALNFFDSMRFDYFIEPSVKHHTVVVNLLSRVGQVDKALNFINKMPETPDFVIWGALFCACRTHKDTKIAKIALQSSCSLNLSIP 499