g.barbadense_cottongen_reftransV1_0097137, g.barbadense_cottongen_reftransV1_0097137 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy Swiss-Prot
Match: Y5457_ARATH (Probable LRR receptor-like serine/threonine-protein kinase At5g45780 OS=Arabidopsis thaliana GN=At5g45780 PE=2 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 2.313e-51
Identity = 170/596 (28.52%), Postives = 265/596 (44.46%), Query Frame = 2

Query: 1376 AFVV-LQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSLG-LSTVFLC*LETDL*S-----VMDGNQGLKRLNEGFQGDNNPG---------LCGTGFPLLRRCGPFDGVNINQVEPFRPHLNNTAPEPGVNSHPSNVQGHCNHTHCSRPSRFPEMPVISGVTTMVFIFTVAGLFAVIHYRRQKQKIGNTSGSSEESFSIDQKKEILRNGSVSPLITLEYSYGWDPLGDGWDNIGFSKEHLDKFRFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSG------------KLQLTSSMRLGAECSRFEDFVDANLKGEFSESMAAKLGEIALRCTNQHPNDRPCMETIIKELKDI 3079
             FVV L++    L+G + T IG L  L+ + LQ N+L G IP  LG L                         ELE LD+  N  SG +P+    SLG L+ +    L  +L S     ++ G  GL  L+  F   + P          + G  F     CGP      +   P R              + + +    N  H S    F    V++ + +++F+F     F V+ +R    ++  +    +  F I   K                                        RF+  E+++AT  FS  N+LG+  F  VYKG L +G+VVA++ +      + E +F   + ++    H NL+RL GFC +    E  L+Y +   G+++  L    G +P LDW  RISI  G A G+ YLH  E   P I+HR++    +L+D  F  ++ D GL K+L     +    V   +G++APEY++TG+ +EKTD+F FGV+IL++++G            K  + S +R      RF + VD +LKGEF + +  ++ E+AL CT  HPN RP M  ++K L+ +
Sbjct:   79 GFVVSLEMASKGLSGILSTSIGELTHLHTLLLQNNQLTGPIPSELGQL------------------------SELETLDLSGNRFSGEIPA----SLGFLTHLNYLRLSRNLLSGQVPHLVAGLSGLSFLDLSFNNLSGPTPNISAKDYRIVGNAF----LCGPASQELCSDATPVR--------------NATGLSEKDNSKHHSLVLSFAFGIVVAFIISLMFLF-----FWVLWHR---SRLSRSHVQQDYEFEIGHLK----------------------------------------RFSFREIQTATSNFSPKNILGQGGFGMVYKGYLPNGTVVAVKRLK-DPIYTGEVQFQTEVEMIGLAVHRNLLRLFGFCMTPE--ERMLVYPYMPNGSVADRLRDNYGEKPSLDWNRRISIALGAARGLVYLH--EQCNPKIIHRDVKAANILLDESFEAIVGDFGLAKLLDQRDSHVTTAVRGTIGHIAPEYLSTGQSSEKTDVFGFGVLILELITGHKMIDQGNGQVRKGMILSWVRTLKAEKRFAEMVDRDLKGEFDDLVLEEVVELALLCTQPHPNLRPRMSQVLKVLEGL 575          

HSP 2 Score: 68.9366 bits (167), Expect = 1.516e-10
Identity = 48/120 (40.00%), Postives = 65/120 (54.17%), Query Frame = 1

Query:  655 PKWVHGNTELRALMELKASLDPTNKVLESWRSDG-DPCSGSFMGVACNEHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIG 1011
            PK V  N E+ ALM +K  +    +VL  W  +  DPC+ + +G  C+    V ++ +  KGLSG LS +I     L  L L  N L+G IP EL QL EL  L L+ N  SG IP+ +G
Sbjct:   33 PKGV--NYEVAALMSVKNKMKDEKEVLSGWDINSVDPCTWNMVG--CSSEGFVVSLEMASKGLSGILSTSIGELTHLHTLLLQNNQLTGPIPSELGQLSELETLDLSGNRFSGEIPASLG 148          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy Swiss-Prot
Match: NIK1_ARATH (Protein NSP-INTERACTING KINASE 1 OS=Arabidopsis thaliana GN=NIK1 PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.014e-49
Identity = 166/584 (28.42%), Postives = 260/584 (44.52%), Query Frame = 2

Query: 1367 NLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSLGLSTVFLC*LETDL*SVMDGNQGLKRLNEGFQGDNNPGLCGTGFPLLRRCGPFDGVNINQVEPFRPHLNNTAPEPGVNSHPSNVQGHCNHTHCSRPSRFPEMPVISGVTTMVFIFTVAGLFAVIHYRRQKQKIGNTSGSSEESFSIDQKKEILRNGSVSPLITLEYSYGWDPLGDGWDNIGFSKEHLDKFRFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGK-------------LQLTSSMRLGAECSRFEDFVDANL--KGEFSESMAAKLGEIALRCTNQHPNDRPCMETIIKELK 3073
            NL    ++ L  N + G IP +IG L +L  + L  N   G+IP S+G L+ L+ L L+ NSL    P +L+++ +L  LD+  N LSG VP                               +   + F    NP +C TG             N   + P   +LN T    GV   P    G  NH           + V S V T+  IF   GLF    + RQ+         ++ +F  D K                         DG  +   S  +L +F F   E++ AT  FS  NLLGK  + +VYKG+L D +VVA++ +        E +F   + +++   H NL+RL GFC ++   E  L+Y + S G+++  +      +PVLDW  R  I  G A G+ YLH  E   P I+HR++    +L+D     ++ D GL K+L     +    V   +G++APEY++TG+ +EKTD+F FG+++L++++G+             + L    ++  E  + E  VD  L  K  + E    ++  +AL CT   P  RP M  +++ L+
Sbjct:  103 NLTNLRIVLLQNNNIKGKIPAEIGRLTRLETLDLSDNFFHGEIPFSVGYLQSLQYLRLNNNSLSGVFPLSLSNMTQLAFLDLSYNNLSGPVP-------------------------------RFAAKTFSIVGNPLICPTGTE--------PDCNGTTLIPMSMNLNQT----GV---PLYAGGSRNHKMA--------IAVGSSVGTVSLIFIAVGLFL---WWRQRH--------NQNTF-FDVK-------------------------DGNHHEEVSLGNLRRFGF--RELQIATNNFSSKNLLGKGGYGNVYKGILGDSTVVAVKRLKDGGALGGEIQFQTEVEMISLAVHRNLLRLYGFCITQ--TEKLLVYPYMSNGSVASRMK----AKPVLDWSIRKRIAIGAARGLVYLH--EQCDPKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELVTGQRAFEFGKAANQKGVMLDWVKKIHQE-KKLELLVDKELLKKKSYDEIELDEMVRVALLCTQYLPGHRPKMSEVVRMLE 584          

HSP 2 Score: 77.7962 bits (190), Expect = 3.483e-13
Identity = 49/126 (38.89%), Postives = 69/126 (54.76%), Query Frame = 1

Query:  655 PKWVHGNTELRALMELKASLDPTNKVLESWRSDG-DPCSGSFMGVACNEHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQ 1029
            PK V  N E++ALM++KASL   + VL++W  D  DPCS +   V C+    V  +    + LSG LSP+I     L  + L  N++ GKIP E+ +L  L  L L+ N   G IP  +G +  LQ
Sbjct:   35 PKGV--NFEVQALMDIKASLHDPHGVLDNWDRDAVDPCSWTM--VTCSSENFVIGLGTPSQNLSGTLSPSITNLTNLRIVLLQNNNIKGKIPAEIGRLTRLETLDLSDNFFHGEIPFSVGYLQSLQ 156          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy Swiss-Prot
Match: GSO1_ARATH (LRR receptor-like serine/threonine-protein kinase GSO1 OS=Arabidopsis thaliana GN=GSO1 PE=2 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 4.413e-49
Identity = 171/590 (28.98%), Postives = 265/590 (44.92%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKR-LDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPS--GNDVSLGLSTVFLC*LETDL*SVMDGNQGLKRLNEGFQGDN---NPGLCGTGFPLLRRCGPFDGVNINQVEPFRPHLNNTAPEPGVNSHPSNVQGHCNHTHCSRPSRFPEMPVISGVTTM-VFIFTVAGLFAVIHYRRQKQKIGNTSGSSEESFSIDQKKEILRNGSVSPLITLEYSYGWDPLGDGWDNIGFSKEHLDKFRFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLD----VEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILA---DDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGKLQLTSSMRLGAECSRF------------EDFVDANLKG--EFSESMAAKLGEIALRCTNQHPNDRP 3043
            + GNL A  VL L  NQ +G++P  +G L +L  + L  N L G+IP  +G L+ L+  LDLS+N+    IP T+  + +LE LD+ +N L+G VP   G+  SLG   V          S  +    LK+    +  D+   N GLCG+    L RC              R   NN   + G+++    +                 +  IS +T + + I  +A  F   H   +K   G+T+ +S  S S    K + RNG+                               K     E++  AT   SE  ++G      VYK  L +G  VA++ I           F + +  L  +RH +LV+L G+C SK  G   LIY++   G++  +L     V +  + +LDW  R+ I  G+A G+ YLH   V  PPIVHR+I    VL+D      + D GL K+L    D    S    + + GY+APEY  + + TEK+D+++ G+++++I++GK+   S      +  R+            +  +D  LK    F E  A ++ EIAL+CT   P +RP
Sbjct:  714 EIGNLGALNVLNLDKNQFSGSLPQAMGKLSKLYELRLSRNSLTGEIPVEIGQLQDLQSALDLSYNNFTGDIPSTIGTLSKLETLDLSHNQLTGEVPGSVGDMKSLGYLNV----------SFNNLGGKLKKQFSRWPADSFLGNTGLCGSP---LSRCN-------------RVRSNNK--QQGLSARSVVI-----------------ISAISALTAIGLMILVIALFFKQRHDFFKKVGHGSTAYTSSSSSSQATHKPLFRNGA------------------------------SKSDIRWEDIMEATHNLSEEFMIGSGGSGKVYKAELENGETVAVKKILWKDDLMSNKSFSREVKTLGRIRHRHLVKLMGYCSSKSEGLNLLIYEYMKNGSIWDWLHEDKPVLEKKKKLLDWEARLRIAVGLAQGVEYLHHDCV--PPIVHRDIKSSNVLLDSNMEAHLGDFGLAKVLTENCDTNTDSNTWFACSYGYIAPEYAYSLKATEKSDVYSMGIVLMEIVTGKMPTDSVFGAEMDMVRWVETHLEVAGSARDKLIDPKLKPLLPFEEDAACQVLEIALQCTKTSPQERP 1226          

HSP 2 Score: 69.3218 bits (168), Expect = 1.602e-10
Identity = 40/95 (42.11%), Postives = 56/95 (58.95%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
            + G L    +L L  N LTG IP+Q+G + QL  ++L  N+L G IP SL +L  L+ LDLS N+L   IP    ++ +L  L + NN LSG +P
Sbjct:  234 ELGRLENLEILNLANNSLTGEIPSQLGEMSQLQYLSLMANQLQGLIPKSLADLGNLQTLDLSANNLTGEIPEEFWNMSQLLDLVLANNHLSGSLP 328          

HSP 3 Score: 67.0106 bits (162), Expect = 1.035e-9
Identity = 48/125 (38.40%), Postives = 70/125 (56.00%), Query Frame = 1

Query:  673 NTELRALMELKASL--DPT-NKVLESWRSDG-DPCSGSFMGVACNEHR--KVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQ 1029
            N +L+ L+E+K SL  +P  +  L  W SD  + CS  + GV C+     +V  ++L G GL+G +SP    F  L  L L  N+L G IP  LS L  L  L+L  N L+G IPSQ+G++  ++
Sbjct:   24 NNDLQTLLEVKKSLVTNPQEDDPLRQWNSDNINYCS--WTGVTCDNTGLFRVIALNLTGLGLTGSISPWFGRFDNLIHLDLSSNNLVGPIPTALSNLTSLESLFLFSNQLTGEIPSQLGSLVNIR 146          

HSP 4 Score: 66.6254 bits (161), Expect = 1.289e-9
Identity = 36/95 (37.89%), Postives = 51/95 (53.68%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
            + GN     V     N L G IP ++G L+ L ++ L  N L G+IP  LG +  L+ L L  N L   IP +LAD+  L+ LD+  N L+G +P
Sbjct:  210 ELGNCSDLTVFTAAENMLNGTIPAELGRLENLEILNLANNSLTGEIPSQLGEMSQLQYLSLMANQLQGLIPKSLADLGNLQTLDLSANNLTGEIP 304          

HSP 5 Score: 66.2402 bits (160), Expect = 1.415e-9
Identity = 39/94 (41.49%), Postives = 54/94 (57.45%), Query Frame = 2

Query: 1364 GNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPS 1645
            GNL    +L L   +LTG IP+Q+G L ++  + LQ N L+G IP  LGN   L     + N L   IP  L  +  LEIL++ NN+L+G +PS
Sbjct:  164 GNLVNLQMLALASCRLTGPIPSQLGRLVRVQSLILQDNYLEGPIPAELGNCSDLTVFTAAENMLNGTIPAELGRLENLEILNLANNSLTGEIPS 257          

HSP 6 Score: 65.4698 bits (158), Expect = 2.957e-9
Identity = 38/100 (38.00%), Postives = 57/100 (57.00%), Query Frame = 2

Query: 1385 VLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSLGLSTVFL 1684
            VL L  N+ +G IP +IG+   L ++ +  N  +G+IPPS+G L+ L  L L  N L   +P +L +  +L ILD+ +N LSG +PS      GL  + L
Sbjct:  436 VLFLYENRFSGEIPQEIGNCTSLKMIDMFGNHFEGEIPPSIGRLKELNLLHLRQNELVGGLPASLGNCHQLNILDLADNQLSGSIPSSFGFLKGLEQLML 535          

HSP 7 Score: 64.3142 bits (155), Expect = 6.395e-9
Identity = 37/95 (38.95%), Postives = 54/95 (56.84%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
            + GN  +  ++ +  N   G IP  IG LK+LN++ L+ N L G +P SLGN   L  LDL+ N L  +IP +   +  LE L + NN+L G +P
Sbjct:  451 EIGNCTSLKMIDMFGNHFEGEIPPSIGRLKELNLLHLRQNELVGGLPASLGNCHQLNILDLADNQLSGSIPSSFGFLKGLEQLMLYNNSLQGNLP 545          

HSP 8 Score: 62.3882 bits (150), Expect = 2.460e-8
Identity = 35/93 (37.63%), Postives = 52/93 (55.91%), Query Frame = 2

Query: 1367 NLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPS 1645
            NL     L L  N L G +P +I +L++L V+ L  NR  G+IP  +GN   LK +D+  N     IPP++  + EL +L +R N L G +P+
Sbjct:  406 NLTNLQWLVLYHNNLEGKLPKEISALRKLEVLFLYENRFSGEIPQEIGNCTSLKMIDMFGNHFEGEIPPSIGRLKELNLLHLRQNELVGGLPA 498          

HSP 9 Score: 60.8474 bits (146), Expect = 7.526e-8
Identity = 42/111 (37.84%), Postives = 56/111 (50.45%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPS-----GNDVSLGLST 1675
            Q G L     L L  N L G IP ++G+   L V     N L+G IP  LG L  L+ L+L+ NSL   IP  L ++ +L+ L +  N L GL+P      GN  +L LS 
Sbjct:  186 QLGRLVRVQSLILQDNYLEGPIPAELGNCSDLTVFTAAENMLNGTIPAELGRLENLEILNLANNSLTGEIPSQLGEMSQLQYLSLMANQLQGLIPKSLADLGNLQTLDLSA 296          

HSP 10 Score: 59.3066 bits (142), Expect = 2.062e-7
Identity = 34/93 (36.56%), Postives = 50/93 (53.76%), Query Frame = 2

Query: 1364 GNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
            G +    +L +  N LTG IP Q+   K+L  + L  N L G IPP LG L  L  L LS N   +++P  L +  +L +L +  N+L+G +P
Sbjct:  620 GKIRELSLLDMSSNALTGTIPLQLVLCKKLTHIDLNNNFLSGPIPPWLGKLSQLGELKLSSNQFVESLPTELFNCTKLLVLSLDGNSLNGSIP 712          

HSP 11 Score: 59.3066 bits (142), Expect = 2.100e-7
Identity = 34/88 (38.64%), Postives = 50/88 (56.82%), Query Frame = 1

Query:  766 SGSFMGVACNEHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQ 1029
            SGS     C+ +  +  + L G  LSG++   ++  + L  L L  NSL+G IP+ L +L ELTDLYL+ N L G++   I N+  LQ
Sbjct:  324 SGSLPKSICSNNTNLEQLVLSGTQLSGEIPVELSKCQSLKQLDLSNNSLAGSIPEALFELVELTDLYLHNNTLEGTLSPSISNLTNLQ 411          

HSP 12 Score: 57.7658 bits (138), Expect = 5.852e-7
Identity = 31/78 (39.74%), Postives = 48/78 (61.54%), Query Frame = 1

Query:  796 EHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQ 1029
            E  ++ ++ L    L G LSP+I+    L  L L++N+L GK+P+E+S L++L  L+L  N  SG IP +IGN   L+
Sbjct:  382 ELVELTDLYLHNNTLEGTLSPSISNLTNLQWLVLYHNNLEGKLPKEISALRKLEVLFLYENRFSGEIPQEIGNCTSLK 459          

HSP 13 Score: 57.3806 bits (137), Expect = 7.223e-7
Identity = 39/108 (36.11%), Postives = 52/108 (48.15%), Query Frame = 2

Query: 1388 LQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDG-----------------------KIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
            L L  N L GN+P  + SL+ L  + L +NRL+G                       +IP  LGN + L RL L  N L   IP TL  I EL +LD+ +N L+G +P
Sbjct:  533 LMLYNNSLQGNLPDSLISLRNLTRINLSHNRLNGTIHPLCGSSSYLSFDVTNNGFEDEIPLELGNSQNLDRLRLGKNQLTGKIPWTLGKIRELSLLDMSSNALTGTIP 640          

HSP 14 Score: 57.3806 bits (137), Expect = 9.157e-7
Identity = 36/93 (38.71%), Postives = 53/93 (56.99%), Query Frame = 2

Query: 1367 NLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPS 1645
            NL +   L L  NQLTG IP+Q+GSL  +  + +  N L G IP +LGNL  L+ L L+   L   IP  L  +  ++ L +++N L G +P+
Sbjct:  117 NLTSLESLFLFSNQLTGEIPSQLGSLVNIRSLRIGDNELVGDIPETLGNLVNLQMLALASCRLTGPIPSQLGRLVRVQSLILQDNYLEGPIPA 209          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy Swiss-Prot
Match: EMS1_ARATH (Leucine-rich repeat receptor protein kinase EMS1 OS=Arabidopsis thaliana GN=EMS1 PE=1 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 1.819e-47
Identity = 166/602 (27.57%), Postives = 267/602 (44.35%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPS--GNDVSLGLSTVFLC*LETDL*SVMDGNQGLKRLNEGFQGDNNPGLCGTGFPLLRRCGPFDGVNINQVEPFRPHLNNTAPEPGVNSHPSNVQGHCNHTHCSRP-------------SRFPEMPVISGVTTMVFIFTVA-GLFAVIHYRRQKQKIGNTSGSSEESFSIDQKKEILRNGSVSPLITLEYSYGWDPLGDGWDNIGFSKEHLDKFRFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGKLQLTSSMR-------LGAECSRFE-----DFVDANLKGEFSESMAAKLGEIALRCTNQHPNDRPCMETIIKELKDI 3079
            + GN      L L  NQL G+IP   G L  L  + L  N+LDG +P SLGNL+ L  +DLSFN+L   +   L+ + +L  L +  N  +G +PS  GN   L                 +D ++ L        G+    +CG   P           N+  +   + +L    P  GV   PS      N   C R              S +    ++ G T +VF+F  +   +A+    +Q+        S  + F +DQ            L  L  S   +PL     NI   ++ L K R  L ++  AT  FS+ N++G   F +VYK  L     VA++ ++    +    EF+  +  L  ++H NLV L G+C      E  L+Y++   G+L  +L  + G   VLDW  R+ I  G A G+ +LH   +  P I+HR+I    +L+D  F P +AD GL ++++    + +  ++   GY+ PEY  + R T K D+++FGVI+L++++GK       +       +G    +       D +D  L     ++   +L +IA+ C  + P  RP M  ++K LK+I
Sbjct:  647 EMGNSLKLQGLNLANNQLNGHIPESFGLLGSLVKLNLTKNKLDGPVPASLGNLKELTHMDLSFNNLSGELSSELSTMEKLVGLYIEQNKFTGEIPSELGNLTQL---------------EYLDVSENL------LSGEIPTKICG--LP-----------NLEFLNLAKNNLRGEVPSDGVCQDPSKALLSGNKELCGRVVGSDCKIEGTKLRSAWGIAGLMLGFTIIVFVFVFSLRRWAMTKRVKQRDDPERMEESRLKGF-VDQN-----------LYFLSGSRSREPLSI---NIAMFEQPLLKVR--LGDIVEATDHFSKKNIIGDGGFGTVYKACLPGEKTVAVKKLSEAKTQGNR-EFMAEMETLGKVKHPNLVSLLGYCSFSE--EKLLVYEYMVNGSLDHWLRNQTGMLEVLDWSKRLKIAVGAARGLAFLHHGFI--PHIIHRDIKASNILLDGDFEPKVADFGLARLISACESHVSTVIAGTFGYIPPEYGQSARATTKGDVYSFGVILLELVTGKEPTGPDFKESEGGNLVGWAIQKINQGKAVDVIDPLLVSVALKNSQLRLLQIAMLCLAETPAKRPNMLDVLKALKEI 1192          

HSP 2 Score: 67.781 bits (164), Expect = 6.082e-10
Identity = 37/83 (44.58%), Postives = 52/83 (62.65%), Query Frame = 2

Query: 1415 GNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSL 1663
            G IP +I SLK L  + L  N+  GKIPP + NL+ L+ LDLS NSL   +P  L+++P+L  LD+ +N  SG +P    +SL
Sbjct:   79 GQIPKEISSLKNLRELCLAGNQFSGKIPPEIWNLKHLQTLDLSGNSLTGLLPRLLSELPQLLYLDLSDNHFSGSLPPSFFISL 161          

HSP 3 Score: 66.6254 bits (161), Expect = 1.073e-9
Identity = 42/103 (40.78%), Postives = 56/103 (54.37%), Query Frame = 2

Query: 1337 YRPS*DIQTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPS 1645
            Y P+   + GN  +   L L  NQLTG IP +IG L  L+V+ L  N   GKIP  LG+   L  LDL  N+L   IP  +  + +L+ L +  N LSG +PS
Sbjct:  463 YLPA---EIGNAASLKRLVLSDNQLTGEIPREIGKLTSLSVLNLNANMFQGKIPVELGDCTSLTTLDLGSNNLQGQIPDKITALAQLQCLVLSYNNLSGSIPS 562          

HSP 4 Score: 58.151 bits (139), Expect = 4.155e-7
Identity = 38/86 (44.19%), Postives = 47/86 (54.65%), Query Frame = 2

Query: 1388 LQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPS 1645
            L L  N L  +IP   G L  L+++ L    L G IPP LGN + LK L LSFNSL   +P  L++IP L       N LSG +PS
Sbjct:  239 LDLSYNPLKCSIPKSFGELHNLSILNLVSAELIGLIPPELGNCKSLKSLMLSFNSLSGPLPLELSEIP-LLTFSAERNQLSGSLPS 323          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy Swiss-Prot
Match: RCH1_ARATH (LRR receptor-like serine/threonine-protein kinase RCH1 OS=Arabidopsis thaliana GN=RCH1 PE=2 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 3.406e-47
Identity = 179/611 (29.30%), Postives = 264/611 (43.21%), Query Frame = 2

Query: 1370 LFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEI-LDVRNNTLSGLVPSGNDVSLGLSTVFLC*--LETDL*SVMDGNQGLKRLNE-------------------GFQGDNNPGLCGTGFPLLRRCGPFDGVNINQVEPFRPHLNNTAPEPGVNSHPSNVQGHCNHTHCSRPSRFPEMPVISGVTTMVFIFTVAGLFAVIHYRRQKQKIGNTSGSSEESFSIDQKKEILRNGSVSPLITLEYSYGWDPLGDGWDNIGFSKEHLDKFRFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSI---------NVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILAD-DIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGKLQLTSSMRLGAE----CSRFEDF--VDANLKGEFS---ESMAAKLGEIALRCTNQHPNDRPCMETIIKELKDI 3079
            L    VL +  N LTG IP  +G L  LN + L  N  +G+IP SLG+   L+ LDLS N++   IP  L DI +L+I L++  N+L G +P        LS + +    L  DL S + G + L  LN                    G + + N GLC  GF   R C             F  + +    + GV+SH   +                    I  + ++  +  V G+ AVI   R KQ I + + S                       T E  + W           F+     K  F +E V    +C  E N++GK     VYK  + +  V+A++ +           T        F   +  L S+RH+N+VR  G C +K      L+YD+ S G+L   L  E  G   L W  R  II G A G+ YLH   V  PPIVHR+I    +LI   F P I D GL K++ D D   S+  ++ + GY+APEY  + + TEK+D++++GV++L++L+GK  +  ++  G        +  D   +D  L+       E M   LG +AL C N  P DRP M+ +   L +I
Sbjct:  538 LTKLQVLDVSSNDLTGKIPDSLGHLISLNRLILSKNSFNGEIPSSLGHCTNLQLLDLSSNNISGTIPEELFDIQDLDIALNLSWNSLDGFIPERISALNRLSVLDISHNMLSGDL-SALSGLENLVSLNISHNRFSGYLPDSKVFRQLIGAEMEGNNGLCSKGF---RSC-------------FVSNSSQLTTQRGVHSHRLRI-------------------AIGLLISVTAVLAVLGVLAVI---RAKQMIRDDNDSE----------------------TGENLWTWQ----------FTP--FQKLNFTVEHV---LKCLVEGNVIGKGCSGIVYKAEMPNREVIAVKKLWPVTVPNLNEKTKSSGVRDSFSAEVKTLGSIRHKNIVRFLGCCWNKNTR--LLMYDYMSNGSLGSLLH-ERSGVCSLGWEVRYKIILGAAQGLAYLHHDCV--PPIVHRDIKANNILIGPDFEPYIGDFGLAKLVDDGDFARSSNTIAGSYGYIAPEYGYSMKITEKSDVYSYGVVVLEVLTGKQPIDPTIPDGLHIVDWVKKIRDIQVIDQGLQARPESEVEEMMQTLG-VALLCINPIPEDRPTMKDVAAMLSEI 1066          

HSP 2 Score: 63.929 bits (154), Expect = 7.961e-9
Identity = 42/116 (36.21%), Postives = 61/116 (52.59%), Query Frame = 2

Query: 1295 C*ENFNLYSLFDINYRPS*DIQTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
            C E  NL+ L+D +   +   + G L     + L  N L G IP +IG +K LN + L  N   G IP S GNL  L+ L LS N++  +IP  L++  +L    +  N +SGL+P
Sbjct:  274 CSELINLF-LYDNDLSGTLPKELGKLQNLEKMLLWQNNLHGPIPEEIGFMKSLNAIDLSMNYFSGTIPKSFGNLSNLQELMLSSNNITGSIPSILSNCTKLVQFQIDANQISGLIP 388          

HSP 3 Score: 60.4622 bits (145), Expect = 8.923e-8
Identity = 35/92 (38.04%), Postives = 53/92 (57.61%), Query Frame = 2

Query: 1367 NLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
            N    V  Q+  NQ++G IP +IG LK+LN+     N+L+G IP  L   + L+ LDLS N L  ++P  L  +  L  L + +N +SG++P
Sbjct:  369 NCTKLVQFQIDANQISGLIPPEIGLLKELNIFLGWQNKLEGNIPDELAGCQNLQALDLSQNYLTGSLPAGLFQLRNLTKLLLISNAISGVIP 460          

HSP 4 Score: 60.4622 bits (145), Expect = 9.963e-8
Identity = 37/96 (38.54%), Postives = 52/96 (54.17%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNT-LSGLVP 1642
            + G+    +V+ L  N L G IP+ +G LK L  + L  N L GKIPP LG+   LK L++  N L + +P  L  I  LE +    N+ LSG +P
Sbjct:  125 EIGDCSELIVIDLSSNSLVGEIPSSLGKLKNLQELCLNSNGLTGKIPPELGDCVSLKNLEIFDNYLSENLPLELGKISTLESIRAGGNSELSGKIP 220          

HSP 5 Score: 58.9214 bits (141), Expect = 2.779e-7
Identity = 33/85 (38.82%), Postives = 53/85 (62.35%), Query Frame = 2

Query: 1388 LQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
            L L  N ++G IP +IG+   L  + L  NR+ G+IP  +G L+ L  LDLS N+L   +P  +++  +L++L++ NNTL G +P
Sbjct:  448 LLLISNAISGVIPLEIGNCTSLVRLRLVNNRITGEIPKGIGFLQNLSFLDLSENNLSGPVPLEISNCRQLQMLNLSNNTLQGYLP 532          

HSP 6 Score: 57.7658 bits (138), Expect = 6.950e-7
Identity = 39/127 (30.71%), Postives = 63/127 (49.61%), Query Frame = 1

Query:  679 ELRALME-LKASLDPTNKVLESWR-SDGDPCSGSFMGVACNEHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQG*FIICFN 1053
            E+ AL+  L +S  P   V   W  SD DPC   ++  + ++++ V  I++    L+    P I+ F  L  L +   +L+G I  E+    EL  + L+ N+L G IPS +G +  LQ    +C N
Sbjct:   39 EVSALISWLHSSNSPPPSVFSGWNPSDSDPCQWPYITCSSSDNKLVTEINVVSVQLALPFPPNISSFTSLQKLVISNTNLTGAISSEIGDCSELIVIDLSSNSLVGEIPSSLGKLKNLQE---LCLN 162          

HSP 7 Score: 57.3806 bits (137), Expect = 7.304e-7
Identity = 38/95 (40.00%), Postives = 54/95 (56.84%), Query Frame = 2

Query: 1367 NLFAFVVLQ---LCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
            N+ +F  LQ   +    LTG I ++IG   +L V+ L  N L G+IP SLG L+ L+ L L+ N L   IPP L D   L+ L++ +N LS  +P
Sbjct:  101 NISSFTSLQKLVISNTNLTGAISSEIGDCSELIVIDLSSNSLVGEIPSSLGKLKNLQELCLNSNGLTGKIPPELGDCVSLKNLEIFDNYLSENLP 195          

HSP 8 Score: 56.9954 bits (136), Expect = 9.827e-7
Identity = 31/96 (32.29%), Postives = 52/96 (54.17%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPS 1645
            + GN    + L L  N L+G +P ++G L+ L  + L  N L G IP  +G ++ L  +DLS N     IP +  ++  L+ L + +N ++G +PS
Sbjct:  270 ELGNCSELINLFLYDNDLSGTLPKELGKLQNLEKMLLWQNNLHGPIPEEIGFMKSLNAIDLSMNYFSGTIPKSFGNLSNLQELMLSSNNITGSIPS 365          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy Swiss-Prot
Match: BAK1_ARATH (BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 OS=Arabidopsis thaliana GN=BAK1 PE=1 SV=2)

HSP 1 Score: 184.111 bits (466), Expect = 3.594e-47
Identity = 95/299 (31.77%), Postives = 167/299 (55.85%), Query Frame = 2

Query: 2219 HLDKF-RFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGK-------------LQLTSSMRLGAECSRFEDFVDANLKGEFSESMAAKLGEIALRCTNQHPNDRPCMETIIKELK 3073
            HL +  RF+L E++ A+  FS  N+LG+  F  VYKG L DG++VA++ +     +  E +F   + +++   H NL+RLRGFC +    E  L+Y + + G+++  L      +P LDWP R  I  G A G+ YLH      P I+HR++    +L+D +F  ++ D GL K++     +    V   +G++APEY++TG+ +EKTD+F +GV++L++++G+             + L   ++   +  + E  VD +L+G + +    +L ++AL CT   P +RP M  +++ L+
Sbjct:  270 HLGQLKRFSLRELQVASDNFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTQGGELQFQTEVEMISMAVHRNLLRLRGFCMTPT--ERLLVYPYMANGSVASCLRERPESQPPLDWPKRQRIALGSARGLAYLHDH--CDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGVMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEALVDVDLQGNYKDEEVEQLIQVALLCTQSSPMERPKMSEVVRMLE 564          

HSP 2 Score: 79.337 bits (194), Expect = 1.131e-13
Identity = 50/123 (40.65%), Postives = 67/123 (54.47%), Query Frame = 1

Query:  664 VHGNTELRALMELKASLDPTNKVLESWRSD-GDPCSGSFMGVACNEHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQ 1029
            V GN E  AL  LK SL   NKVL+SW +    PC+  +  V CN    V  + L    LSG+L   +     L  L L+ N+++G IP++L  L EL  L L +NNLSG IPS +G +  L+
Sbjct:   23 VSGNAEGDALSALKNSLADPNKVLQSWDATLVTPCT--WFHVTCNSDNSVTRVDLGNANLSGQLVMQLGQLPNLQYLELYSNNITGTIPEQLGNLTELVSLDLYLNNLSGPIPSTLGRLKKLR 143          

HSP 3 Score: 73.9442 bits (180), Expect = 4.622e-12
Identity = 42/103 (40.78%), Postives = 60/103 (58.25%), Query Frame = 2

Query: 1355 IQTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSL 1663
            +Q G L     L+L  N +TG IP Q+G+L +L  + L  N L G IP +LG L+ L+ L L+ NSL   IP +L  +  L++LD+ NN L+G +P     SL
Sbjct:   86 MQLGQLPNLQYLELYSNNITGTIPEQLGNLTELVSLDLYLNNLSGPIPSTLGRLKKLRFLRLNNNSLSGEIPRSLTAVLTLQVLDLSNNPLTGDIPVNGSFSL 188          

HSP 4 Score: 60.4622 bits (145), Expect = 7.360e-8
Identity = 36/92 (39.13%), Postives = 48/92 (52.17%), Query Frame = 2

Query: 1409 LTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSLGLSTVFL 1684
            L+G +  Q+G L  L  + L  N + G IP  LGNL  L  LDL  N+L   IP TL  + +L  L + NN+LSG +P      L L  + L
Sbjct:   80 LSGQLVMQLGQLPNLQYLELYSNNITGTIPEQLGNLTELVSLDLYLNNLSGPIPSTLGRLKKLRFLRLNNNSLSGEIPRSLTAVLTLQVLDL 171          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy Swiss-Prot
Match: SERK4_ARATH (Somatic embryogenesis receptor kinase 4 OS=Arabidopsis thaliana GN=SERK4 PE=1 SV=2)

HSP 1 Score: 183.726 bits (465), Expect = 5.293e-47
Identity = 99/299 (33.11%), Postives = 167/299 (55.85%), Query Frame = 2

Query: 2219 HLDKF-RFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGK-------------LQLTSSMRLGAECSRFEDFVDANLKGEFSESMAAKLGEIALRCTNQHPNDRPCMETIIKELK 3073
            HL +  RF L E+  AT  FS  N+LG+  F  VYKG L DG++VA++ +     K  E +F   + +++   H NL+RLRGFC +    E  L+Y + + G+++  L     G P LDWP R  I  G A G+ YLH     K  I+HR++    +L+D +F  ++ D GL K++  +  +    V   +G++APEY++TG+ +EKTD+F +GV++L++++G+             + L   ++   +  + E  VDA L+G++ E+   +L ++AL CT     +RP M  +++ L+
Sbjct:  275 HLGQLKRFTLRELLVATDNFSNKNVLGRGGFGKVYKGRLADGNLVAVKRLKEERTKGGELQFQTEVEMISMAVHRNLLRLRGFCMTPT--ERLLVYPYMANGSVASCLRERPEGNPALDWPKRKHIALGSARGLAYLHDHCDQK--IIHRDVKAANILLDEEFEAVVGDFGLAKLMNYNDSHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGVMLLELITGQKAFDLARLANDDDIMLLDWVKEVLKEKKLESLVDAELEGKYVETEVEQLIQMALLCTQSSAMERPKMSEVVRMLE 569          

HSP 2 Score: 85.1149 bits (209), Expect = 1.770e-15
Identity = 53/126 (42.06%), Postives = 71/126 (56.35%), Query Frame = 1

Query:  664 VHGNTELRALMELKASL---DPTNKVLESWRSD-GDPCSGSFMGVACNEHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQ 1029
            V GN E  AL +LK SL   DP N VL+SW +    PC+  +  V CN   KV  + L    LSGKL P +     L  L L+ N+++G+IP+EL  L EL  L L  N++SG IPS +G +  L+
Sbjct:   27 VAGNAEGDALTQLKNSLSSGDPANNVLQSWDATLVTPCT--WFHVTCNPENKVTRVDLGNAKLSGKLVPELGQLLNLQYLELYSNNITGEIPEELGDLVELVSLDLYANSISGPIPSSLGKLGKLR 150          

HSP 3 Score: 70.8626 bits (172), Expect = 4.469e-11
Identity = 42/102 (41.18%), Postives = 58/102 (56.86%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSL 1663
            + G L     L+L  N +TG IP ++G L +L  + L  N + G IP SLG L  L+ L L+ NSL   IP TL  + +L++LD+ NN LSG +P     SL
Sbjct:   94 ELGQLLNLQYLELYSNNITGEIPEELGDLVELVSLDLYANSISGPIPSSLGKLGKLRFLRLNNNSLSGEIPMTLTSV-QLQVLDISNNRLSGDIPVNGSFSL 194          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy Swiss-Prot
Match: BRL2_ARATH (Serine/threonine-protein kinase BRI1-like 2 OS=Arabidopsis thaliana GN=BRL2 PE=1 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 8.786e-47
Identity = 158/597 (26.47%), Postives = 260/597 (43.55%), Query Frame = 2

Query: 1388 LQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSLGLSTVFLC*LETDL*SVMDGNQGLKRLNEGFQGDNNPGLCGTGFPLLRRCGPFDGVNINQVEPFRPHLNNTAPEPGVNSHPSNVQGHCNHTHCSRPSRFPEMPVI-----SGVTTMVFIFTVAGLFAVIHYRRQKQKIGNTSGSSEESFSIDQKKEILRNGSVSPLITLEYSYGWDPLGDGWDNIGFSKEHLDKFRFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEP--VLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKIL-ADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGK----------LQLTSSMRLGAECSRFEDFVDANLKGE-FSESMAAKLG--------------EIALRCTNQHPNDRPCMETIIKELKDI 3079
            L L  NQL G IP +IG +  L V+ L +N+L G+IP ++G L+ L   D S N L   IP + +++  L  +D+ NN L+G +P    +S   +T                           Q  NNPGLCG   P  +                           G N  P+  +      H +R + +    V+     +    ++ ++ +A           K      + +S  ++ I+++KE L                         N+   +  L K +F+  ++  AT  FS  +++G   F  V+K  L+DGS VAI+ +   SC+ +  EF+  +  L  ++H NLV L G+C  K   E  L+Y+F   G+L + L     GE   +L W  R  I  G A G+ +LH + +  P I+HR++    VL+D      ++D G+ +++ A D   S   ++   GY+ PEY  + R T K D+++ GV++L+ILSGK            L    ++ A   +  + +D +L  E  SES+  K G              EIALRC +  P+ RP M  ++  L+++
Sbjct:  616 LDLSYNQLRGKIPDEIGEMIALQVLELSHNQLSGEIPFTIGQLKNLGVFDASDNRLQGQIPESFSNLSFLVQIDLSNNELTGPIPQRGQLSTLPAT---------------------------QYANNPGLCGVPLPECKN--------------------------GNNQLPAGTEEGKRAKHGTRAASWANSIVLGVLISAASVCILIVWAIAVRARRRDADDAKMLHSLQAVNSATTWKIEKEKEPLS-----------------------INVATFQRQLRKLKFS--QLIEATNGFSAASMIGHGGFGEVFKATLKDGSSVAIKKLIRLSCQGDR-EFMAEMETLGKIKHRNLVPLLGYC--KIGEERLLVYEFMQYGSLEEVLHGPRTGEKRRILGWEERKKIAKGAAKGLCFLHHNCI--PHIIHRDMKSSNVLLDQDMEARVSDFGMARLISALDTHLSVSTLAGTPGYVPPEYYQSFRCTAKGDVYSIGVVMLEILSGKRPTDKEEFGDTNLVGWSKMKAREGKHMEVIDEDLLKEGSSESLNEKEGFEGGVIVKEMLRYLEIALRCVDDFPSKRPNMLQVVASLREL 1129          

HSP 2 Score: 63.5438 bits (153), Expect = 9.137e-9
Identity = 36/87 (41.38%), Postives = 49/87 (56.32%), Query Frame = 2

Query: 1388 LQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNL-RMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPS 1645
            L L  N   G IP   G LK L  + L +NRL G IPP +G+  R L+ L LS+N+    IP +L+    L+ LD+ NN +SG  P+
Sbjct:  233 LNLSYNNFDGQIPKSFGELKLLQSLDLSHNRLTGWIPPEIGDTCRSLQNLRLSYNNFTGVIPESLSSCSWLQSLDLSNNNISGPFPN 319          

HSP 3 Score: 62.003 bits (149), Expect = 3.459e-8
Identity = 34/71 (47.89%), Postives = 41/71 (57.75%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIP 1570
            + G + A  VL+L  NQL+G IP  IG LK L V     NRL G+IP S  NL  L ++DLS N L   IP
Sbjct:  630 EIGEMIALQVLELSHNQLSGEIPFTIGQLKNLGVFDASDNRLQGQIPESFSNLSFLVQIDLSNNELTGPIP 700          

HSP 4 Score: 61.2326 bits (147), Expect = 5.330e-8
Identity = 33/86 (38.37%), Postives = 48/86 (55.81%), Query Frame = 2

Query: 1385 VLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
             + L  N L G IP +IG+L++L      YN + G+IPP +G L+ LK L L+ N L   IPP   +   +E +   +N L+G VP
Sbjct:  403 TIDLSLNYLNGTIPPEIGNLQKLEQFIAWYNNIAGEIPPEIGKLQNLKDLILNNNQLTGEIPPEFFNCSNIEWVSFTSNRLTGEVP 488          

HSP 5 Score: 60.4622 bits (145), Expect = 8.425e-8
Identity = 31/85 (36.47%), Postives = 49/85 (57.65%), Query Frame = 2

Query: 1388 LQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
            L+L  N +TG IP  I    +L  + L  N L+G IPP +GNL+ L++    +N++   IPP +  +  L+ L + NN L+G +P
Sbjct:  380 LRLPDNLVTGEIPPAISQCSELRTIDLSLNYLNGTIPPEIGNLQKLEQFIAWYNNIAGEIPPEIGKLQNLKDLILNNNQLTGEIP 464          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy Swiss-Prot
Match: NIK2_ARATH (Protein NSP-INTERACTING KINASE 2 OS=Arabidopsis thaliana GN=NIK2 PE=1 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 1.791e-46
Identity = 100/292 (34.25%), Postives = 164/292 (56.16%), Query Frame = 2

Query: 2234 RFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGKLQL---TSSMRLGA---------ECSRFEDFVDANLKGEFSESMAAKLGEIALRCTNQHPNDRPCMETIIKELK 3073
            RFN +E++SAT  FS  NL+GK  F +VYKG L DGS++A++ +   +    E +F   L +++   H NL+RL GFC +    E  L+Y + S G+++  L      +PVLDW TR  I  G   G+ YLH  E   P I+HR++    +L+D  F  ++ D GL K+L  +  +    V   +G++APEY++TG+ +EKTD+F FG+++L++++G   L    ++ + GA         +  + E  VD +LK  +      ++ ++AL CT   P  RP M  +++ L+
Sbjct:  299 RFNFKELQSATSNFSSKNLVGKGGFGNVYKGCLHDGSIIAVKRLKDINNGGGEVQFQTELEMISLAVHRNLLRLYGFCTTSS--ERLLVYPYMSNGSVASRLK----AKPVLDWGTRKRIALGAGRGLLYLH--EQCDPKIIHRDVKAANILLDDYFEAVVGDFGLAKLLDHEESHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGLRALEFGKAANQRGAILDWVKKLQQEKKLEQIVDKDLKSNYDRIEVEEMVQVALLCTQYLPIHRPKMSEVVRMLE 582          

HSP 2 Score: 66.2402 bits (160), Expect = 1.100e-9
Identity = 38/93 (40.86%), Postives = 54/93 (58.06%), Query Frame = 2

Query: 1364 GNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
            GNL     + L  N +TGNIP +IG L +L  + L  N   G+IP +L   + L+ L ++ NSL   IP +LA++ +L  LD+  N LSG VP
Sbjct:  102 GNLTNLQTVLLQNNYITGNIPHEIGKLMKLKTLDLSTNNFTGQIPFTLSYSKNLQYLRVNNNSLTGTIPSSLANMTQLTFLDLSYNNLSGPVP 194          

HSP 3 Score: 65.4698 bits (158), Expect = 2.319e-9
Identity = 38/91 (41.76%), Postives = 52/91 (57.14%), Query Frame = 2

Query: 1376 AFVV-LQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPS 1645
             FV+ L+     L+G + + IG+L  L  V LQ N + G IP  +G L  LK LDLS N+    IP TL+    L+ L V NN+L+G +PS
Sbjct:   81 GFVIRLEAPSQNLSGTLSSSIGNLTNLQTVLLQNNYITGNIPHEIGKLMKLKTLDLSTNNFTGQIPFTLSYSKNLQYLRVNNNSLTGTIPS 171          

HSP 4 Score: 58.151 bits (139), Expect = 4.093e-7
Identity = 40/120 (33.33%), Postives = 62/120 (51.67%), Query Frame = 1

Query:  673 NTELRALMELKASLDPTNKVLESWRSDG-DPCSGSFMGVACNEHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQ 1029
            N E+ AL+ +K+SL   + VL +W     DPCS +   + C++   V  +    + LSG LS +I     L  + L  N ++G IP E+ +L +L  L L+ NN +G IP  +     LQ
Sbjct:   40 NFEVVALIGIKSSLTDPHGVLMNWDDTAVDPCSWNM--ITCSDGF-VIRLEAPSQNLSGTLSSSIGNLTNLQTVLLQNNYITGNIPHEIGKLMKLKTLDLSTNNFTGQIPFTLSYSKNLQ 156          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy Swiss-Prot
Match: Y1534_ARATH (Probable LRR receptor-like serine/threonine-protein kinase At1g53420 OS=Arabidopsis thaliana GN=At1g53420 PE=2 SV=2)

HSP 1 Score: 184.882 bits (468), Expect = 2.522e-46
Identity = 99/290 (34.14%), Postives = 165/290 (56.90%), Query Frame = 2

Query: 2237 FNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGK-----------LQLTSSMRLGAECSRFEDFVDANLKGEFSESMAAKLGEIALRCTNQHPNDRPCMETIIKELK 3073
            F+L +++ AT  F   N +G+  F  VYKG L DG+++A++ ++ T  K    EF+  + ++++L H NLV+L G CC +G G+  L+Y+F    +L++ L      +  LDWPTR  I  G+A G+ YLH  E S+  IVHR+I    VL+D Q +P I+D GL K+  +D  + + +++   GY+APEY   G  T+K D+++FG++ L+I+ G+             L   + +  E +   + VD  L  E++   A  + +IA+ CT+  P +RP M  ++K L+
Sbjct:  612 FSLRQIKIATNNFDSANRIGEGGFGPVYKGKLFDGTIIAVKQLS-TGSKQGNREFLNEIGMISALHHPNLVKLYG-CCVEG-GQLLLVYEFVENNSLARALFGPQETQLRLDWPTRRKICIGVARGLAYLH--EESRLKIVHRDIKATNVLLDKQLNPKISDFGLAKLDEEDSTHISTRIAGTFGYMAPEYAMRGHLTDKADVYSFGIVALEIVHGRSNKIERSKNNTFYLIDWVEVLREKNNLLELVDPRLGSEYNREEAMTMIQIAIMCTSSEPCERPSMSEVVKMLE 896          

HSP 2 Score: 61.6178 bits (148), Expect = 3.380e-8
Identity = 33/91 (36.26%), Postives = 52/91 (57.14%), Query Frame = 2

Query: 1370 LFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVP 1642
            +   V + L  N+LTG IP + G++  L  + L+ N+L G++P  LGNL  ++++ LS N+    IP T A +  L    V +N LSG +P
Sbjct:  109 VLPLVNIWLLGNRLTGPIPKEFGNITTLTSLVLEANQLSGELPLELGNLPNIQQMILSSNNFNGEIPSTFAKLTTLRDFRVSDNQLSGTIP 199          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy TrEMBL
Match: A0A061DTY0_THECC (Leucine-rich repeat receptor protein kinase OS=Theobroma cacao GN=TCM_005524 PE=4 SV=1)

HSP 1 Score: 645.58 bits (1664), Expect = 0.000e+0
Identity = 329/572 (57.52%), Postives = 411/572 (71.85%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSLGLSTVFLC*LETDL*SVMDGNQGLKRLNEGFQGDNNPGLCGTGFPLLRRCGPFDGVNINQVEPFRPH-LNNTAPEPGVNSHPSNVQGHCNHTHCSRPSRFPEMPVISGVTTMVFIFTVAGLFAVIHYRRQKQKIGNTSGSSEESFSIDQKKEILRNGSVSPLITLEYSYGWDPLGDGWDNIGFSKEHLDKFRFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGKLQLTSSMRLGAECSRFEDFVDANLKGEFSESMAAKLGEIALRCTNQHPNDRPCMETIIKEL 3070
            Q GN+    VLQLC N+LTG+IPTQ+GSLK+LNV+ALQYNRL G IP SLG+L ML RLDLSFNSLF  +P  LAD P L++LD+RNNTLSG VP                              LKRLN GFQ +NN GLCG GF  L+ C     +  ++ EPF+P  LN T      +  P++    C+ T+CSR S+   + VISG+  +V + TVA LF    YRR+KQKIG+   +S+   S DQ KE+ R  S SPLI+LEYS GWDPL  G    GFS+E  + F FNLEEVE AT+CFSE+NLLGK+NFS++YKG LRDGSVV ++ I  TSCKS+E+EF+ GL +LTSL+HENLVRLRGFCCSKGRGECFLIYDF   GNL ++LDV+ G   VL+W TR+SII GIA GIGYLH S+ SKP +VH+NIS EKVLID++ +PL++DSGLHK+  DDIV+S LK SAAMGYLAPEY TTGRFTEK+D++AFG+I+ QILSGK ++T  +RLGAE  + EDF+D NL+G++S+S A KLG IAL CT++ P+ RP M+ +I+EL
Sbjct:  132 QIGNMANLQVLQLCYNKLTGSIPTQLGSLKKLNVLALQYNRLTGAIPASLGDLEMLTRLDLSFNSLFGPVPVKLADAPMLKVLDIRNNTLSGNVPPV----------------------------LKRLNSGFQYENNLGLCGVGFSDLKDCADSGKIYRDRPEPFKPSGLNRTDIPESADLQPAD----CSKTNCSRSSKSLTIGVISGMVGVVLVLTVAALFTFAWYRRRKQKIGSAFDTSDGRLSTDQAKEVYRR-SASPLISLEYSNGWDPLDKGRSGNGFSQEVFESFMFNLEEVERATQCFSEVNLLGKSNFSAIYKGFLRDGSVVVVKCIAKTSCKSDESEFLHGLKILTSLKHENLVRLRGFCCSKGRGECFLIYDFVHNGNLLQHLDVKPGSGKVLEWSTRVSIIYGIAKGIGYLHGSKGSKPALVHQNISAEKVLIDNRSNPLLSDSGLHKLRTDDIVFSTLKASAAMGYLAPEYTTTGRFTEKSDVYAFGMILFQILSGKRKITQLIRLGAESCKVEDFIDMNLEGKYSDSEATKLGRIALLCTHESPSHRPSMDNVIQEL 670          

HSP 2 Score: 158.688 bits (400), Expect = 7.124e-37
Identity = 80/127 (62.99%), Postives = 98/127 (77.17%), Query Frame = 1

Query:  676 TELRALMELKASLDPTNKVLESWRSDGDPCS-GSFMGVACNEHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQG*FIICFN 1053
            TE   L+++KASLDP N+ L SW    DPCS GSF GVACNE   VANISLQGKGLSG++  A+ G K L+GLYLH+N+LSG IP+E++ L +L+DLYLNVN+LSG IPSQIGNMA LQ    +C+N
Sbjct:   22 TERELLLQIKASLDPQNRFLSSWTPYTDPCSSGSFDGVACNEEGHVANISLQGKGLSGQIPAALGGLKSLTGLYLHFNALSGGIPKEIATLSQLSDLYLNVNDLSGEIPSQIGNMANLQV-LQLCYN 147          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy TrEMBL
Match: F6HAL9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0022g01620 PE=4 SV=1)

HSP 1 Score: 644.81 bits (1662), Expect = 0.000e+0
Identity = 329/573 (57.42%), Postives = 409/573 (71.38%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSLGLSTVFLC*LETDL*SVMDGNQGLKRLNEGFQGDNNPGLCGTGFPLLRRCGPFDGVNINQVEPFRPHLNNTAPEPGVNSHPSNVQGHCNHTHCSRPSRFPEMPVISGVTTMVFIFTVAGLFAVIHYRRQKQKIGNTSGSSEESFSIDQKKEILR-NGSVSPLITLEYSYGWDPLGDGWDNIGFSKEHLDKFRFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGKLQLTSSMRLGAECSRFEDFVDANLKGEFSESMAAKLGEIALRCTNQHPNDRPCMETIIKELK 3073
            + G +    VLQLC NQLTG+IPTQ+GSLK+L+V+ALQ N+L G IP SLG+L ML RLDLSFN LF +IP  LAD+  LE+LD+RNNTLSG +P                              LKRLN GFQ  NN  LCG GF  L+ C   D  N ++ EP+    N  +   G     +NVQ HC+  HCS PS+    PV+ G+  +    +  G+ +   YRR+KQK+G++   S+   S DQ KE+ R NGS  PL++LEYS GWDPL DG +  GF +E    FRFNLEEVESAT+ FSE+NLLGK+NFS++YKG+LRDGS+VAI+SIN TSCKSE+AEF+KGL +LTSLRHENLVRLRG CCSKGRGECFLIYDF   GNL  YLD++DG   VL+W TR+S+I+GIA GI YLH  +++KP +VH+NIS EKVLID + +PL++DSGLHK+L DDIV+SALK SAAMGYLAPEY TTGRFTEK+D++AFGV++ QILSGK + TSS+ LGAE  RF+DF+DANL G FSE  AAKL  IAL CT++ P +RP MET+I EL+
Sbjct:  135 ELGKMATLQVLQLCYNQLTGSIPTQLGSLKKLSVLALQSNQLTGAIPASLGDLGMLVRLDLSFNRLFGSIPRRLADVVSLEVLDIRNNTLSGKIPPA----------------------------LKRLNGGFQYKNNARLCGDGFSYLKVCNSLDLTNQSRPEPYGAGSNTQST--GDIPETANVQLHCDKAHCSNPSKSSHAPVVVGMVVVTIALSAIGILSFAQYRRRKQKLGSSFDISDSRLSTDQGKEVYRKNGS--PLVSLEYSNGWDPLADGRNYGGFPQEVFQSFRFNLEEVESATQHFSEVNLLGKSNFSAIYKGILRDGSLVAIKSINKTSCKSEDAEFLKGLNILTSLRHENLVRLRGLCCSKGRGECFLIYDFIPNGNLLSYLDLKDGDSKVLEWSTRVSMISGIAKGIEYLHGYKLNKPALVHQNISAEKVLIDQRLNPLLSDSGLHKLLTDDIVFSALKASAAMGYLAPEYTTTGRFTEKSDLYAFGVLVFQILSGKRKFTSSIHLGAETCRFQDFIDANLHGRFSEYEAAKLARIALMCTHESPIERPSMETVIHELE 675          

HSP 2 Score: 181.03 bits (458), Expect = 5.036e-44
Identity = 88/134 (65.67%), Postives = 108/134 (80.60%), Query Frame = 1

Query:  652 VPKWVHGNTELRALMELKASLDPTNKVLESWRSDGDPCSGSFMGVACNEHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQG*FIICFN 1053
            VP  VHG +ELR+LM +K++LDP N  L SW  +GDPC GSF GVACNE  +VANISLQGKGL+GKLSPAIAG K L+GLYLHYNSL G+IP E++ L +L+DLYLNVNNLSG+IP+++G MA LQ    +C+N
Sbjct:   18 VPLLVHGTSELRSLMVIKSTLDPHNLFLSSWTINGDPCDGSFEGVACNERGQVANISLQGKGLTGKLSPAIAGLKHLTGLYLHYNSLYGEIPVEIANLTQLSDLYLNVNNLSGTIPAELGKMATLQV-LQLCYN 150          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy TrEMBL
Match: I1LGL6_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=2)

HSP 1 Score: 639.417 bits (1648), Expect = 0.000e+0
Identity = 327/580 (56.38%), Postives = 405/580 (69.83%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSLGLSTVFLC*LETDL*SVMDGNQGLKRLNEGFQGDNNPGLCGTGFPLLRRCGPFDGVNINQV------EPFRPHLNNTAPEPGVNSHPSNVQGHCNHTHCSRPSRFPEMPVISGVTTMVFIFTVAGLFAVIHYRRQKQKIGNT-SGSSEESFSIDQKKEILRNGSVSPLITLEYSYGWDPLGDGW--DNIGFSKEHLDKFRFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGKLQLTSSMRLGAECSRFEDFVDANLKGEFSESMAAKLGEIALRCTNQHPNDRPCMETIIKEL 3070
            +  N+ +  VLQL  NQL G IPTQ+ SLKQL+ +ALQYN+L G+IP SLGNL  L RL+LSFN+    +P TLA I  LE+LD++NN+LSG+VPS                             LKRL EGFQG NNPGLCG GF  LR C     +N+N +      +P   + +   PEP        VQ HC  THCS+  RFP+  + +GV  +   F  AG      YRRQKQ+I NT S SSE   S+DQ KE     S S L+ ++Y  GWD L +G   D  G S E+L++FRFN++EVESAT  FSE NLL ++ F++ YKGVLRDGS+VAIRSI+VT CK+EEAEFVKGL LLTSLRHENLVRLRGFCCS+ RGECFLIYDFA+ GNLS+YLD+EDG   VL+W  R+SII GIA GIGYLH +E SKP IVH+NISVE VL+D+QF+PLI D+GL  +LADD+V+SALKVSAAMGYLAPEY+TTGRFTEK+DI+AFGVIILQ+LSGK  + SS+R   E  RF+D VD NLKG +S+S AA L ++A++CTN+ P++RP M  +I+EL
Sbjct:  138 EIANMASLQVLQLGDNQLVGTIPTQMSSLKQLSTLALQYNKLTGQIPLSLGNLEKLSRLNLSFNNFSGTVPATLAHIEHLEVLDIQNNSLSGIVPS----------------------------ALKRLGEGFQGANNPGLCGVGFSTLRACNKDQDLNVNHIDTSDGDQPKNSNSSKALPEPAY------VQLHCGQTHCSKSRRFPQTVITAGVVIVTLTFICAGFLTFFRYRRQKQRISNTLSSSSEGKVSLDQPKEFYTK-SPSALVNIDYYSGWDQLSNGQNADAGGLSNEYLNQFRFNVDEVESATHYFSEANLLNRSKFAAGYKGVLRDGSLVAIRSISVTCCKTEEAEFVKGLNLLTSLRHENLVRLRGFCCSRSRGECFLIYDFATMGNLSQYLDIEDGSSHVLEWSKRVSIIKGIANGIGYLHSNEESKPTIVHQNISVENVLLDYQFNPLIRDAGLPMLLADDVVFSALKVSAAMGYLAPEYITTGRFTEKSDIYAFGVIILQVLSGKTTIGSSIRTAVESFRFDDSVDTNLKGIYSKSEAATLSKLAIQCTNELPDERPTMVDVIQEL 682          

HSP 2 Score: 189.119 bits (479), Expect = 1.386e-46
Identity = 92/127 (72.44%), Postives = 103/127 (81.10%), Query Frame = 1

Query:  649 YVPKWVHGNTELRALMELKASLDPTNKVLESWRSDGDPCSGSFMGVACNEHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQ 1029
            +VP    G  ELRALMELK+SLDP  K+L SW SDGDPCSG F GVACN+HRKVANISLQGKGLSG LSPA+A  KCLSGLYLHYN+LSG+IP  +S L EL DLYL+VN LSG+IP +I NMA LQ
Sbjct:   20 HVPHMAVGTAELRALMELKSSLDPEGKILGSWISDGDPCSGFFEGVACNDHRKVANISLQGKGLSGWLSPALAELKCLSGLYLHYNNLSGEIPPHISNLTELLDLYLDVNTLSGTIPPEIANMASLQ 146          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy TrEMBL
Match: A0A0B2RPF8_GLYSO (Protein NSP-INTERACTING KINASE 2 OS=Glycine soja GN=glysoja_004731 PE=4 SV=1)

HSP 1 Score: 639.032 bits (1647), Expect = 0.000e+0
Identity = 327/580 (56.38%), Postives = 405/580 (69.83%), Query Frame = 2

Query: 1358 QTGNLFAFVVLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSLGLSTVFLC*LETDL*SVMDGNQGLKRLNEGFQGDNNPGLCGTGFPLLRRCGPFDGVNINQV------EPFRPHLNNTAPEPGVNSHPSNVQGHCNHTHCSRPSRFPEMPVISGVTTMVFIFTVAGLFAVIHYRRQKQKIGNT-SGSSEESFSIDQKKEILRNGSVSPLITLEYSYGWDPLGDGW--DNIGFSKEHLDKFRFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGKLQLTSSMRLGAECSRFEDFVDANLKGEFSESMAAKLGEIALRCTNQHPNDRPCMETIIKEL 3070
            +  N+ +  VLQL  NQL G IPTQ+ SLKQL+ +ALQYN+L G+IP SLGNL  L RL+LSFN+    +P TLA I  LE+LD++NN+LSG+VPS                             LKRL EGFQG NNPGLCG GF  LR C     +N+N +      +P   + +   PEP        VQ HC  THCS+  RFP+  + +GV  +   F  AG      YRRQKQ+I NT S SSE   S+DQ KE     S S L+ ++Y  GWD L +G   D  G S E+L++FRFN++EVESAT  FSE NLL ++ F++ YKGVLRDGS+VAIRSI+VT CK+EEAEFVKGL LLTSLRHENLVRLRGFCCS+ RGECFLIYDFA+ GNLS+YLD+EDG   VL+W  R+SII GIA GIGYLH +E SKP IVH+NISVE VL+D+QF+PLI D+GL  +LADD+V+SALKVSAAMGYLAPEY+TTGRFTEK+DI+AFGVIILQ+LSGK  + SS+R   E  RF+D VD NLKG +S+S AA L ++A++CTN+ P++RP M  +I+EL
Sbjct:  138 EIANMASLQVLQLGDNQLVGTIPTQMSSLKQLSTLALQYNKLTGQIPLSLGNLEKLSRLNLSFNNFSGTVPATLAHIEHLEVLDIQNNSLSGIVPS----------------------------ALKRLGEGFQGANNPGLCGVGFSTLRACNKDQDLNVNHIDTSDGDQPKNSNSSKALPEPAY------VQLHCGQTHCSKSRRFPQTVITAGVVIVTLTFICAGFLTFFRYRRQKQRISNTLSSSSEGKVSLDQPKEFYTK-SPSALVNIDYYSGWDQLSNGQNADAGGLSHEYLNQFRFNVDEVESATHYFSEANLLNRSKFAAGYKGVLRDGSLVAIRSISVTCCKTEEAEFVKGLNLLTSLRHENLVRLRGFCCSRSRGECFLIYDFATMGNLSQYLDIEDGSSHVLEWSKRVSIIKGIANGIGYLHSNEESKPTIVHQNISVENVLLDYQFNPLIRDAGLPMLLADDVVFSALKVSAAMGYLAPEYITTGRFTEKSDIYAFGVIILQVLSGKTTIGSSIRTAVESFRFDDSVDTNLKGIYSKSEAATLSKLAIQCTNELPDERPTMVDVIQEL 682          

HSP 2 Score: 189.119 bits (479), Expect = 1.313e-46
Identity = 92/127 (72.44%), Postives = 103/127 (81.10%), Query Frame = 1

Query:  649 YVPKWVHGNTELRALMELKASLDPTNKVLESWRSDGDPCSGSFMGVACNEHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQ 1029
            +VP    G  ELRALMELK+SLDP  K+L SW SDGDPCSG F GVACN+HRKVANISLQGKGLSG LSPA+A  KCLSGLYLHYN+LSG+IP  +S L EL DLYL+VN LSG+IP +I NMA LQ
Sbjct:   20 HVPHMAVGTAELRALMELKSSLDPEGKILGSWISDGDPCSGFFEGVACNDHRKVANISLQGKGLSGWLSPALAELKCLSGLYLHYNNLSGEIPPHISNLTELLDLYLDVNTLSGTIPPEIANMASLQ 146          

BLAST of g.barbadense_cottongen_reftransV1_0097137 vs. ExPASy TrEMBL
Match: W9R8I9_9ROSA (LRR receptor-like serine/threonine-protein kinase RCH1 OS=Morus notabilis GN=L484_025574 PE=4 SV=1)

HSP 1 Score: 637.106 bits (1642), Expect = 0.000e+0
Identity = 326/570 (57.19%), Postives = 405/570 (71.05%), Query Frame = 2

Query: 1385 VLQLCCNQLTGNIPTQIGSLKQLNVVALQYNRLDGKIPPSLGNLRMLKRLDLSFNSLFDAIPPTLADIPELEILDVRNNTLSGLVPSGNDVSLGLSTVFLC*LETDL*SVMDGNQGLKRLNEGFQGDNNPGLCGTGFPLLRRCGPFDGVNINQVEPFRPH--LNNTAPEPGVNSHPS------NVQGHCNHTHCSRPSRFPEMPVISGVTTMVFIFTVAGLFAVIHYRRQKQKIGNTSGSSEESFSIDQKKEILRNGSVSPLITLEYSYGWDPLGDGWDNIGFSKEHLDKFRFNLEEVESATRCFSELNLLGKTNFSSVYKGVLRDGSVVAIRSINVTSCKSEEAEFVKGLYLLTSLRHENLVRLRGFCCSKGRGECFLIYDFASKGNLSKYLDVEDGGEPVLDWPTRISIINGIATGIGYLHKSEVSKPPIVHRNISVEKVLIDHQFSPLIADSGLHKILADDIVYSALKVSAAMGYLAPEYVTTGRFTEKTDIFAFGVIILQILSGKLQLTSSMRLGAECSRFEDFVDANLKGEFSESMAAKLGEIALRCTNQHPNDRPCMETIIKEL 3070
            VLQLC N LTG+IP Q+ +LK+L+V+ALQYN+L G IP SLG L+ L RLDLSFNSLF ++P  LAD P L++LD+RNN+LSG VP                            Q LKRLN GFQ  NNP LCG GF  L  C   DG + N+ EP++P     N  P    N          N+   C+ T CS+ S+ P++ V  GV  +   FTV+GLF    YRR+KQKIG+   SS+   S DQ KE+    S SPLI+LEYS GWDP+  G    GFS+E L+ F FNLEEVE AT+CFSE+NLL K+NFS++YKG+LRDGSVVA++ I  TSCKS+EAEF+KGL +LTSL+HENLVRLRGFCCSKGRGECFLIYDF   G+L +YLDVE+G    L+W TR+SIINGIA GI YLH ++ +KP +VH+NIS EK LID  + P+I++SGLHK+LADDI++S LK SAAMGYLAPEY TTGRFT K+D++AFG+IILQILSGK ++T  +R GAE  RFEDF+D NL+G+FSES AAKLG++AL CTN+ PN RP ME +++EL
Sbjct:  147 VLQLCYNTLTGSIPLQLRNLKRLSVLALQYNQLTGAIPASLGELKALTRLDLSFNSLFGSVPAKLADAPMLQVLDIRNNSLSGTVP----------------------------QALKRLNGGFQYKNNPHLCGVGFSDLHVCSASDGWDPNRPEPYQPSNLSANGFPHSAQNRTAEDLPESVNLNPDCSRTSCSKTSKSPQVGVALGVIGLFVAFTVSGLFTFSWYRRRKQKIGSAFDSSDSRLSTDQAKEVYMK-SASPLISLEYSNGWDPVAKGCS--GFSQEVLESFMFNLEEVERATQCFSEVNLLEKSNFSAIYKGILRDGSVVAVKCIAKTSCKSDEAEFLKGLKILTSLKHENLVRLRGFCCSKGRGECFLIYDFIPNGSLLQYLDVEEGSGTALEWSTRVSIINGIAKGIAYLHGNKGNKPAMVHQNISAEKALIDTNYKPMISNSGLHKLLADDIIFSLLKASAAMGYLAPEYTTTGRFTGKSDVYAFGMIILQILSGKRKITQFVRQGAEFCRFEDFIDQNLEGKFSESEAAKLGKLALLCTNESPNHRPSMENVMQEL 685          

HSP 2 Score: 156.762 bits (395), Expect = 3.176e-36
Identity = 73/120 (60.83%), Postives = 89/120 (74.17%), Query Frame = 1

Query:  694 MELKASLDPTNKVLESWRSDGDPCSGSFMGVACNEHRKVANISLQGKGLSGKLSPAIAGFKCLSGLYLHYNSLSGKIPQELSQLQELTDLYLNVNNLSGSIPSQIGNMAGLQG*FIICFN 1053
            M LKASLDP N+ L SW    +PC G F GVAC+E  +V NISLQGKGL G++  A+ G K L+GLYLH+NSL+G+IP+E++ L ELTDLYLNVNNLSG IP Q  NM  LQG   +C+N
Sbjct:   34 MALKASLDPQNQFLTSWTRSANPCGGDFEGVACDERGRVVNISLQGKGLWGRIPAAVGGLKSLTGLYLHFNSLNGEIPKEITNLSELTDLYLNVNNLSGEIPPQFANMPNLQGVLQLCYN 153