g.barbadense_cottongen_reftransV1_0091917, g.barbadense_cottongen_reftransV1_0091917 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP249_ARATH (Pentatricopeptide repeat-containing protein At3g22690 OS=Arabidopsis thaliana GN=PCMP-H56 PE=2 SV=1)

HSP 1 Score: 429.869 bits (1104), Expect = 5.378e-131
Identity = 259/808 (32.05%), Postives = 411/808 (50.87%), Query Frame = -2

Query:  922 TALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQME---LAERVFLFMDKKNV-VSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELF-SLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAK-------------------------------NGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQ-GVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3234
            ++LK C    +L    + H    K G+  DV   + LV    + G  E    A+ VF   +       +N+L+ GYA  G   + + LF  M  S +   K+T    L +C        G+  H L++K G   D FV   L+  Y++CG  +   KVF  + E N+V+W++MI     +   ++A +LF  ++R + V+PN  T   +    A L D    E V+A I   G E   ++ +AL+ MYMK  ++    R+F E  + N    N++ S             +F+ M+  G +PD  +  S + SCS L NI +G   H ++++NG +  + +  +LID+Y K                               NG +  A   F  + E+++ SW  +I+G  Q    E+AI+ F  MQ Q GV  +  T+ +  S+C ++  L+  + ++    K G   DV + +++VDM++ CG  E A + F  + + D  +W   +          + +E F  M+++GL+PD V F+  L+ACS+ G V +GKE F S+  + G+ P   H  CM+D+LGRAG   E    I+DM +  N +IW ++L  CR+ GN ++   AAEK+  L P     Y+LL+N++A+ GRW D+ +VR  M   G++K PG S + + G+ H F S D  HP+   I   L E+ +++   G+VP   ++L DV + EK+  L  H+E+LA+A+ LIS N   TIRI KNL +C DCH F K  S V  +EI++RD N FH+ + G CSC D+
Sbjct:   37 SSLKNCKTIDEL---KMFHRSLTKQGLDNDVSTITKLVARSCELGTRESLSFAKEVFENSESYGTCFMYNSLIRGYASSGLCNEAILLFLRMMNSGISPDKYTFPFGLSACAKSRAKGNGIQIHGLIVKMGYAKDLFVQNSLVHFYAECGELDSARKVFDEMSERNVVSWTSMICGYARRDFAKDAVDLFFRMVRDEEVTPNSVTMVCVISACAKLEDLETGEKVYAFIRNSGIEVNDLMVSALVDMYMKCNAIDVAKRLFDEYGASNLDLCNAMASNYVRQGLTREALGVFNLMMDSGVRPDRISMLSAISSCSQLRNILWGKSCHGYVLRNGFESWDNICNALIDMYMKCHRQDTAFRIFDRMSNKTVVTWNSIVAGYVENGEVDAAWETFETMPEKNIVSWNTIISGLVQGSLFEEAIEVFCSMQSQEGVNADGVTMMSIASACGHLGALDLAKWIYYYIEKNGIQLDVRLGTTLVDMFSRCGDPESAMSIFNSLTNRDVSAWTAAIGAMAMAGNAERAIELFDDMIEQGLKPDGVAFVGALTACSHGGLVQQGKEIFYSMLKLHGVSPEDVHYGCMVDLLGRAGLLEEAVQLIEDMPMEPNDVIWNSLLAACRVQGNVEMAAYAAEKIQVLAPERTGSYVLLSNVYASAGRWNDMAKVRLSMKEKGLRKPPGTSSIQIRGKTHEFTSGDESHPEMPNIEAMLDEVSQRASHLGHVPDLSNVLMDVDEKEKIFMLSRHSEKLAMAYGLISSNKGTTIRIVKNLRVCSDCHSFAKFASKVYNREIILRDNNRFHYIRQGKCSCGDF 841          

HSP 2 Score: 211.46 bits (537), Expect = 4.337e-55
Identity = 186/714 (26.05%), Postives = 323/714 (45.24%), Query Frame = -2

Query: 1543 LEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGV---FGYACKVLDRMPERDV-VSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMT-ESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRS--------------------------VQNG-----WRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLA-EGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMK 3570
            L  +K  K     L +C +   L   K  H  + K+G D D+     LV    + G      +A +V +          + +L  G  + G   + + L+  M   G+ P+ +     L AC+ S     G  +HG  VK+G   D+FV +SLV  YA+CG+++ A +VF  M ++NVVSW +++ GYA +  A   ++LF  M  + E+  +  T+  V+ +C  L +L  G   ++ +  +G E ++ +   L+D+Y KC   +   ++F      N+   +AM      QG  +EA  +F+LM   GV P++ +  S     + L + L+ +  H  + ++GFES   + NALI MYMK                             V+NG     W  F+ M   N  SWN+++SG   G   +    +F  M + EG   D  T  SI  +C +L  +     ++ +I KNG++ +  +GT+L+D++++ G    A  +F  L  RD+ +WTA I   A +  +E+AI+ F+ M  QG+ P+       L++CS+  +++ G ++ +SM    G S +      +VD+    G +EEA                                   +++ D  +EP++V + ++L+AC   G V E   Y      V     T  +   + ++   AG++N++      MK
Sbjct:   26 LNQSKCTKATPSSLKNCKTIDEL---KMFHRSLTKQGLDNDVSTITKLVARSCELGTRESLSFAKEVFENSESYGTCFMYNSLIRGYASSGLCNEAILLFLRMMNSGISPDKYTFPFGLSACAKSRAKGNGIQIHGLIVKMGYAKDLFVQNSLVHFYAECGELDSARKVFDEMSERNVVSWTSMICGYARRDFAKDAVDLFFRMVRDEEVTPNSVTMVCVISACAKLEDLETGEKVYAFIRNSGIEVNDLMVSALVDMYMKCNAIDVAKRLFDEYGASNLDLCNAMASNYVRQGLTREALGVFNLMMDSGVRPDRISMLSAISSCSQLRNILWGKSCHGYVLRNGFESWDNICNALIDMYMKCHRQDTAFRIFDRMSNKTVVTWNSIVAGYVENGEVDAAWETFETMPEKNIVSWNTIISGLVQGSLFEEAIEVFCSMQSQEGVNADGVTMMSIASACGHLGALDLAKWIYYYIEKNGIQLDVRLGTTLVDMFSRCGDPESAMSIFNSLTNRDVSAWTAAIGAMAMAGNAERAIELFDDMIEQGLKPDGVAFVGALTACSHGGLVQQGKEIFYSMLKLHGVSPEDVHYGCMVDLLGRAGLLEEA----------------------------------VQLIEDMPMEPNDVIWNSLLAACRVQGNV-EMAAYAAEKIQVLAPERTGSY-VLLSNVYASAGRWNDMAKVRLSMK 700          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP108_ARATH (Putative pentatricopeptide repeat-containing protein At1g68930 OS=Arabidopsis thaliana GN=PCMP-H22 PE=3 SV=1)

HSP 1 Score: 426.402 bits (1095), Expect = 7.844e-131
Identity = 239/726 (32.92%), Postives = 387/726 (53.31%), Query Frame = -2

Query:  922 TESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDE-----------FVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELF-SLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVF-------------------------------KEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 2970
              ++ R  K    N++++  Y     +  I H+  +     +             F    LL  YSK GL  +    F+++ + + V W+ +I+     G +  A + + ++MR    +  + T  ++  ++++ G     + +H  + K GFES +++ + L+ MY  +  + +  +VF                               + M   + +    +   +QNG         F +M  +G K D   F S+L +C  L  I  G Q+HA II+   +D+  VG++LID+Y K   L  A+ +F ++ ++++ SWTA++ GY Q+  +E+A+K F  MQR G+ P+++TL   +S+C+N++ LE G   H  AI +G    V VS+S+V +Y  CG I+++   F  M   D VSW  M+  Y Q  + ++ ++ F  M+  GL+PD VT   V+SACS  G V +G+ YF  +T+ +GIVP+I H +CMID+  R+G+  E   FI  M    + + W T+L  CR  GN ++G+ AAE L EL+P   + Y LL++I+A+KG+W+ V ++R  M    VKKEPG SW+   G++H F + D   P   +IY KL+EL  K I  GY P T  + +DV +  K++ L +H+ERLA+AF LI +   + IR+ KNL +C DCH+  K +S V  +EI+VRDA  FH FK G CSC D+
Sbjct:   18 ARNQSRYVKMIHGNIIRALPYPETFLYNNIVHAYALMKSSTYARRVFDRIPQPNLFSWNNLLLAYSKAGLISEMESTFEKLPDRDGVTWNVLIEGYSLSGLVGAAVKAYNTMMRDFSANLTRVTLMTMLKLSSSNGHVSLGKQIHGQVIKLGFESYLLVGSPLLYMYANVGCISDAKKVFYGLDDRNTVMYNSLMGGLLACGMIEDALQLFRGMEKDSVSWAAMIKGLAQNG-LAKEAIECFREMKVQGLKMDQYPFGSVLPACGGLGAINEGKQIHACIIRTNFQDHIYVGSALIDMYCKCKCLHYAKTVFDRMKQKNVVSWTAMVVGYGQTGRAEEAVKIFLDMQRSGIDPDHYTLGQAISACANVSSLEEGSQFHGKAITSGLIHYVTVSNSLVTLYGKCGDIDDSTRLFNEMNVRDAVSWTAMVSAYAQFGRAVETIQLFDKMVQHGLKPDGVTLTGVISACSRAGLVEKGQRYFKLMTSEYGIVPSIGHYSCMIDLFSRSGRLEEAMRFINGMPFPPDAIGWTTLLSACRNKGNLEIGKWAAESLIELDPHHPAGYTLLSSIYASKGKWDSVAQLRRGMREKNVKKEPGQSWIKWKGKLHSFSADDESSPYLDQIYAKLEELNNKIIDNGYKPDTSFVHHDVEEAVKVKMLNYHSERLAIAFGLIFVPSGQPIRVGKNLRVCVDCHNATKHISSVTGREILVRDAVRFHRFKDGTCSCGDF 742          

HSP 2 Score: 181.415 bits (459), Expect = 9.555e-46
Identity = 132/511 (25.83%), Postives = 238/511 (46.58%), Query Frame = -2

Query: 2116 LYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKD-GVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVV------------------------------SWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKN-GLKDNNLVGTSLIDLYAKNGFLADAELLFTQL-IERDLFSWTALIA 3546
            LY+ I+H  A   S + A+ V  +I +    P+L  WN+L+ AY+K G+        +++P+RD V+W  L  G    G     +  Y +M +D         L+T LK  S +  +  G  +HG+ +K+G    + VGS L+ +YA  G +  A++VF  +D +N V                              SW A++ G A  G A + +  F+ M    L+  ++   +VL +C  L  +  G   H+ +I+T  +   +VG  L+D+Y KC        VF R+++ N+V+W+AM+    + G+ +EA ++F  M+  G+ P+ +T        AN+         H      G    + +SN+L+ +Y K   + +  R+F EM+  ++ S    + + +Q G+  +    +F KM+  G KPD  T T ++ +CS    ++ G +    +    G+  +    + +IDL++++G L +A      +    D   WT L++
Sbjct:   43 LYNNIVHAYALMKSSTYARRVFDRIPQ----PNLFSWNNLLLAYSKAGLISEMESTFEKLPDRDGVTWNVLIEGYSLSGLVGAAVKAYNTMMRDFSANLTRVTLMTMLKLSSSNGHVSLGKQIHGQVIKLGFESYLLVGSPLLYMYANVGCISDAKKVFYGLDDRNTVMYNSLMGGLLACGMIEDALQLFRGMEKDSVSWAAMIKGLAQNGLAKEAIECFREMKVQGLKMDQYPFGSVLPACGGLGAINEGKQIHACIIRTNFQDHIYVGSALIDMYCKCKCLHYAKTVFDRMKQKNVVSWTAMVVGYGQTGRAEEAVKIFLDMQRSGIDPDHYTLGQAISACANVSSLEEGSQFHGKAITSGLIHYVTVSNSLVTLYGKCGDIDDSTRLFNEMNVRDAVSWTAMVSAYAQFGRAVET-IQLFDKMVQHGLKPDGVTLTGVISACSRAGLVEKGQRYFKLMTSEYGIVPSIGHYSCMIDLFSRSGRLEEAMRFINGMPFPPDAIGWTTLLS 548          

HSP 3 Score: 143.665 bits (361), Expect = 1.439e-33
Identity = 84/305 (27.54%), Postives = 159/305 (52.13%), Query Frame = -2

Query: 2695 FKEKTKLEGAKRLKL-YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWG-----LIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCLDEQGQIQ 3588
            F+E  K++G K  +  +  +L  C   G+++  K +H  II+  F   +++ ++L++ Y K     YA  V DRM +++VVSWTA+  G    G   + + ++  M++ G+ P+ + L  A+ AC+    L  G+  HG+ +  G++  V V +SLV LY KCG ++ + R+F  M+ ++ VSW A+++ YA  G A + + LF  M +  L+    TL+ V+ +C+    +  G     L+     I     H      C++DL+S+ G  E+ ++    +   P+ + W+ ++     +G ++
Sbjct:  258 FRE-MKVQGLKMDQYPFGSVLPACGGLGAINEGKQIHACIIRTNFQDHIYVGSALIDMYCKCKCLHYAKTVFDRMKQKNVVSWTAMVVGYGQTGRAEEAVKIFLDMQRSGIDPDHYTLGQAISACANVSSLEEGSQFHGKAITSGLIHYVTVSNSLVTLYGKCGDIDDSTRLFNEMNVRDAVSWTAMVSAYAQFGRAVETIQLFDKMVQHGLKPDGVTLTGVISACSRAGLVEKGQRYFKLMTSEYGIVPSIGHYS----CMIDLFSRSGRLEEAMRFINGMPFPPDAIGWTTLLSACRNKGNLE 557          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PPR45_ARATH (Pentatricopeptide repeat-containing protein At1g15510, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H73 PE=3 SV=1)

HSP 1 Score: 428.713 bits (1101), Expect = 3.197e-130
Identity = 263/803 (32.75%), Postives = 424/803 (52.80%), Query Frame = -2

Query:  925 GLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTE-SELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSD-CSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQD 3327
            GL   G   + + L  SM++  V  +    V  ++ C        G+ ++   +     L V +G++ + ++ + G +  A  VF  M ++N+ SWN L+ GYA +G   + + L+  M     ++   +T   VL++C  + +L  G   H  V++ G E D  V   L+ +Y KCG  +    +F R+   +I++W+AMI    E G   E  ELF  MR   V P+  T  S+      LGDR     +HA +   GF  +I + N+L  MY+   S +   ++F  M   +  SW +++SG +     D+    +  M  +  KPD  T  ++L +C+ L ++  G ++H   IK  L    +V  +LI++Y+K   +  A  +F  +  +++ SWT++IAG   ++ C E  I  F    +  + PN  TL   L++C+ +  L  G+ +H+  ++ G   D F+ ++++DMY  CG +  A + F      D  SWN++L GY +  QG  V+E F  M+   + PDE+TFI++L  CS    V +G  YF  + + +G+ P +KH AC++D+LGRAG+  E   FI+ M VT +P +W  +L  CR+H    LGE +A+ +FEL+     +YILL N++A  G+W +V +VR +M   G+  + GCSWV V G+VH F S D  HP+  EI   L+   EK    G    ++    D ++  + E    H+ER A+AF LI+  P   I + KNL +CE+CHD +K +S  +++EI VRDA  FHHFK G CSC D
Sbjct:   68 GLCANGKLEEAMKLLNSMQELRVAVDEDVFVALVRLCEWKRAQEEGSKVYSIALSSMSSLGVELGNAFLAMFVRFGNLVDAWYVFGKMSERNLFSWNVLVGGYAKQGYFDEAMCLYHRMLWVGGVKPDVYTFPCVLRTCGGIPDLARGKEVHVHVVRYGYELDIDVVNALITMYVKCGDVKSARLLFDRMPRRDIISWNAMISGYFENGMCHEGLELFFAMRGLSVDPDLMTLTSVISACELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVSWTTMISGYEYNFLPDKAIDTYRMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAIKARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEALI--FLRQMKMTLQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDFLPNALLDMYVRCGRMNTAWSQFNSQ-KKDVTSWNILLTGYSERGQGSMVVELFDRMVKSRVRPDEITFISLLCGCSKSQMVRQGLMYFSKMED-YGVTPNLKHYACVVDLLGRAGELQEAHKFIQKMPVTPDPAVWGALLNACRIHHKIDLGELSAQHIFELDKKSVGYYILLCNLYADCGKWREVAKVRRMMKENGLTVDAGCSWVEVKGKVHAFLSDDKYHPQTKEINTVLEGFYEKMSEVGLTKISESSSMDETEISRDEIFCGHSERKAIAFGLINTVPGMPIWVTKNLSMCENCHDTVKFISKTVRREISVRDAEHFHHFKDGECSCGD 866          

HSP 2 Score: 201.445 bits (511), Expect = 8.851e-52
Identity = 147/546 (26.92%), Postives = 263/546 (48.17%), Query Frame = -2

Query: 1816 LHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSM-KKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMML 3447
            + L N+ +  + + G    A  V  +M ER++ SW  L  G   +GY  + + LY  M    GV P+ +     L+ C    DL  G  +H   V+ G  LD+ V ++L+ +Y KCG ++ A  +F  M +++++SWNA+++GY   G   + L LF  M    +     TL++V+ +C  L +   G   H+ VI TG   D  V   L  +Y   G   +  K+F R++  +IV+W+ MI   +      +A + + +M    V P++ T A++    A LGD      +H    K    S +I++N LI MY K + +     +F  +   N  SW S+++G +    C     IF + +    +P+  T T+ L +C+ +  +  G ++HAH+++ G+  ++ +  +L+D+Y + G +  A   F    ++D+ SW  L+ GY++       ++ F+ M +  V P+  T  + L  CS   M+  G +  S     G + ++   + +VD+    G ++EA      M V+ D   W  +L
Sbjct:  129 VELGNAFLAMFVRFGNLVDAWYVFGKMSERNLFSWNVLVGGYAKQGYFDEAMCLYHRMLWVGGVKPDVYTFPCVLRTCGGIPDLARGKEVHVHVVRYGYELDIDVVNALITMYVKCGDVKSARLLFDRMPRRDIISWNAMISGYFENGMCHEGLELFFAMRGLSVDPDLMTLTSVISACELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVSWTTMISGYEYNFLPDKAIDTYRMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAIKARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEAL-IFLRQMKMTLQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDFLPNALLDMYVRCGRMNTAWSQFNSQ-KKDVTSWNILLTGYSERGQGSMVVELFDRMVKSRVRPDEITFISLLCGCSKSQMVRQGLMYFSKMEDYGVTPNLKHYACVVDLLGRAGELQEAHKFIQKMPVTPDPAVWGALL 672          

HSP 3 Score: 192.2 bits (487), Expect = 8.449e-49
Identity = 125/473 (26.43%), Postives = 225/473 (47.57%), Query Frame = -2

Query: 2119 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQL-IERDLFSWTALI 3534
            +L  C     L+  K VH  +++ G++ D+ + N+L+  Y K G    A  + DRMP RD++SW A+ SG    G  ++ L+L+ +M+   V P+   L + + AC L  D   G  +H   +  G  +D+ V +SL  +Y   G    AE++F  M++K++VSW  +++GY       K ++ ++ M +  ++  + T++ VL +C  L +L  G+  H L IK        V   L+++YSKC   +  L +F  I   N+++W+++I  L    +  EA      M+   + PN  T  +     A +G  +  + +HA + + G   +  L NAL+ MY++   +   W  F       ++    L   S+ G+       +F +M+    +PD  TF S+L  CS    ++ G    + +   G+  N      ++DL  + G L +A     ++ +  D   W AL+
Sbjct:  202 VLRTCGGIPDLARGKEVHVHVVRYGYELDIDVVNALITMYVKCGDVKSARLLFDRMPRRDIISWNAMISGYFENGMCHEGLELFFAMRGLSVDPDLMTLTSVISACELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVSWTTMISGYEYNFLPDKAIDTYRMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAIKARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEALIFLRQMKMT-LQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDFLPNALLDMYVRCGRMNTAWSQFNSQKKDVTSWNILLTGYSERGQG-SMVVELFDRMVKSRVRPDEITFISLLCGCSKSQMVRQGLMYFSKMEDYGVTPNLKHYACVVDLLGRAGELQEAHKFIQKMPVTPDPAVWGALL 672          

HSP 4 Score: 184.496 bits (467), Expect = 2.498e-46
Identity = 134/498 (26.91%), Postives = 244/498 (49.00%), Query Frame = -2

Query: 1543 LNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELF-SLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKE-YFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMK 3030
            L+G    G   + + L   M E  +   +     +++ C +      G   +S+ + +       +G   L ++ + G   D   VF ++ E N+ +W+ ++    +QG   EA  L+  ++   GV P+ +TF  +      + D    + VH  + ++G+E +I + NALI MY+K   V++   +F  M   +  SWN+++SG      C  G  +F  M      PD+ T TS++ +C  L + + G  +HA++I  G   +  V  SL  +Y   G   +AE LF+++  +D+ SWT +I+GY  +   +KAI  +  M +  V P+  T+A  LS+C+ +  L+ G  LH +AIKA     V V++++++MY+ C CI++A   F  +   + +SW  ++ G   + +  + L   R M    L+P+ +T  A L+AC+ +G +  GKE +   L    G+   + +   ++D+  R G+ N   S     K
Sbjct:   66 LHGLCANGKLEEAMKLLNSMQELRVAVDEDVFVALVRLCEWKRAQEEGSKVYSIALSSMSSLGVELGNAFLAMFVRFGNLVDAWYVFGKMSERNLFSWNVLVGGYAKQGYFDEAMCLYHRMLWVGGVKPDVYTFPCVLRTCGGIPDLARGKEVHVHVVRYGYELDIDVVNALITMYVKCGDVKSARLLFDRMPRRDIISWNAMISGYFENGMCHEGLELFFAMRGLSVDPDLMTLTSVISACELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVSWTTMISGYEYNFLPDKAIDTYRMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAIKARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEALIFLRQM-KMTLQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDFLPN--ALLDMYVRCGRMNTAWSQFNSQK 560          

HSP 5 Score: 134.035 bits (336), Expect = 2.120e-30
Identity = 89/306 (29.08%), Postives = 151/306 (49.35%), Query Frame = -2

Query: 2623 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASI 3540
            + ++  C   G     + +H  +I  GF  D+ + NSL   Y   G +  A K+  RM  +D+VSWT + SG          +D Y  M +D V P+   +   L AC+   DL  G  LH   +K  ++  V V ++L+++Y+KC  ++ A  +F  + +KNV+SW +++ G  L     + L   + M  + L+ +  TL+  L +C  +  L  G   H+ V++TG   D+F+   LLD+Y +CG        F   Q+ ++ +W+ ++    E+GQ     ELF  M    V P++ TF S+
Sbjct:  301 TSVISACELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVSWTTMISGYEYNFLPDKAIDTYRMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAIKARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEALIFLRQMKMT-LQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDFLPNALLDMYVRCGRMNTAWSQFNS-QKKDVTSWNILLTGYSERGQGSMVVELFDRMVKSRVRPDEITFISL 604          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP168_ARATH (Pentatricopeptide repeat-containing protein At2g22070 OS=Arabidopsis thaliana GN=PCMP-H41 PE=3 SV=1)

HSP 1 Score: 424.091 bits (1089), Expect = 1.682e-129
Identity = 245/701 (34.95%), Postives = 369/701 (52.64%), Query Frame = -2

Query:  922 LLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMK---------------IRSVQNGWRVFKEMXXXXXXXXXXXXXXSQ----------------NGKTCD-RGPSIFHKMLAEGF-KPDICTFTSILRSCSNLLNIKFGHQVHAHII--------------------------------KNGLKDNNLVG-TSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAF-LGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 2823
            +L  YSK G  + T + F ++ + + V+W+ MI      GQ  +A  +   M  +G+ P Q T  ++    A        + VH+ I K G    + +SN+L+ MY K               +R + + W     +         ++    Q                N +  D R   IF KML +    PD  T  S+L +C+NL  +  G Q+H+HI+                                + G KD  + G T+L+D Y K G +  A+ +F  L +RD+ +WTA+I GY Q     +AI  F  M   G  PN++TLA  LS  S++A L +G+ +H  A+K+G    V VS++++ MYA  G I  A  AF L     DTVSW  M+    QH    + LE F  ML +GL PD +T++ V SAC++ G VN+G++YFD + +V  I+PT+ H ACM+D+ GRAG   E + FI+ M +  + + W ++L  CR+H N  LG+ AAE+L  L P  +  Y  LAN+++A G+WE+  ++R  M    VKKE G SW+ V  +VH+F   DG HP+  EIY+ +K++ ++    GYVP T  +L+D+ ++ K + L HH+E+LA+AF LIS     T+RI KNL +C DCH  +K +S ++ +EI+VRD   FHHFK G CSC+DY
Sbjct:   86 VLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIVGYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISS-WNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFSACTHAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLLSACRVHKNIDLGKVAAERLLLLEPENSGAYSALANLYSACGKWEEAAKIRKSMKDGRVKKEQGFSWIEVKHKVHVFGVEDGTHPEKNEIYMTMKKIWDEIKKMGYVPDTASVLHDLEEEVKEQILRHHSEKLAIAFGLISTPDKTTLRIMKNLRVCNDCHTAIKFISKLVGREIIVRDTTRFHHFKDGFCSCRDY 785          

HSP 2 Score: 187.963 bits (476), Expect = 1.093e-47
Identity = 135/516 (26.16%), Postives = 245/516 (47.48%), Query Frame = -2

Query: 2113 WNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKC-------------------------------GQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGM-TESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLK---------------------------------VFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMX--XXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSL------IDLYAKNGFLADAELLFTQL-IERDLFSWTALIAG 3438
            WN++++AY+KRG     C+  D++P+RD VSWT +  G  N G  +  + +   M K+G+ P    L   L + + +  +  G  +H   VK+G+  +V V +SL+++YAKC                               GQM+LA   F  M ++++V+WN++++G+  +G   + L++F  M  +S L   +FTL++VL +C  L  L  G   HS ++ TG +    V   L+ +YS+CG  E   +                                 +F  +++ ++VAW+AMI   ++ G   EA  LF  M   G  PN +T A++  VA++L    + + +H    K G    + +SNALI MY K  ++ +  R F  +       +  + +++ +Q+G   +    +F  ML EG +PD  T+  +  +C++   +  G Q       + +KD + +  +L      +DL+ + G L +A+    ++ IE D+ +W +L++ 
Sbjct:   83 WNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIVGYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGH-AEEALELFETMLMEGLRPDHITYVGVFSACTHAGLVNQGRQYF-----DMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLLSA 592          

HSP 3 Score: 154.836 bits (390), Expect = 4.558e-37
Identity = 139/597 (23.28%), Postives = 259/597 (43.38%), Query Frame = -2

Query: 1735 KACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAG-------------------------------KILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCG------------LAED-------------------TLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFS-LMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWR---------------------------------VFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQL-IERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSS-IVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMMLFGYLQHRQ-GLKVLETFRMMLDKGLEPD 3225
            K+ + S   F   L+H   +K G++  V++ ++L+++Y+K G    A ++F  M  +   SWN +L+ Y+ +GD                                 K + +   M +  +  ++FTL+NVL S      +  G   HS ++K G   +  V   LL++Y+KCG            +  D                    +  F+++ E +IV W++MI   +++G    A ++FS ++R   +SP++ T AS+    ANL      + +H+ I   GF+   I+ NALI+MY +   V+   R                                 +F  +   +  +W +++ G +   +     ++F  M+  G +P+  T  ++L   S+L ++  G Q+H   +K+G   +  V  +LI +YAK G +  A   F  +  ERD  SWT++I   AQ   +E+A++ F  M  +G+ P++ T     S+C++  ++  G+    M              + +VD++   G ++EA+     M +  D V+W  +L     H+   L  +   R++L   LEP+
Sbjct:   22 KSVNKSNGRFTAQLVHCRVIKSGLMFSVYLMNNLMNVYSKTGYALHARKLFDEMPLRTAFSWNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIVGYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFSACTHAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLLSACRVHKNIDLGKVAAERLLL---LEPE 615          

HSP 4 Score: 137.502 bits (345), Expect = 1.424e-31
Identity = 100/380 (26.32%), Postives = 168/380 (44.21%), Query Frame = -2

Query: 2599 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRM-------------------------------PERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVL-PNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAER---------------------------------VFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLG 3540
            + +L   A+   + + K VH  I+K G   ++ + NSL+N YAK G    A  V DRM                                ERD+V+W ++ SG    GY    LD++  M +D +L P+   L + L AC+    L  G  +H   V  G  +   V ++L+ +Y++CG +E A R                                 +F+ +  ++VV+W A++ GY   G  G+ +NLF+ M     R + +TL+ +L   + L +L+ G   H   +K+G  +   V   L+ +Y+K G      + F  I+ E + V+W++MI  L + G  +EA ELF  M  +G+ P+  T+  +     + G
Sbjct:  150 TNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFSACTHAG 529          

HSP 5 Score: 119.398 bits (298), Expect = 7.048e-26
Identity = 96/349 (27.51%), Postives = 150/349 (42.98%), Query Frame = -2

Query: 1540 PDICTFTSILRSCSNLL-------NIKFGHQ-VHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFT-------------------------------QLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANC-------------------------------GCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETF-RMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLT----NVFGIVPTIKHCACMIDILGRAGKFNEVESFI-----KDMKV 2346
            P   + +++L  C+NLL       N +F  Q VH  +IK+GL  +  +  +L+++Y+K G+   A  LF                                QL +RD  SWT +I GY       KAI+    M ++G+ P  FTL   L+S +    +E G+ +HS  +K G  G+V VS+S+++MYA C                               G ++ A A F  M   D V+WN M+ G+ Q    L+ L+ F +M+ D  L PD  T  +VLSAC+ +  +  GK+    +     ++ GIV        +I +  R G        I     KD+K+
Sbjct:    4 PVPLSLSTLLELCTNLLQKSVNKSNGRFTAQLVHCRVIKSGLMFSVYLMNNLMNVYSKTGYALHARKLFDEMPLRTAFSWNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIVGYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIV-----LNALISMYSRCGGVETARRLIEQRGTKDLKI 347          

HSP 6 Score: 106.301 bits (264), Expect = 6.281e-22
Identity = 81/319 (25.39%), Postives = 139/319 (43.57%), Query Frame = -2

Query: 2725 SKILHDCASKGSLSSAKAVHGKIIKKGFD-------------------------------PDLHL--WNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVF-LFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEH-------DEFVG-----CCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMI 3540
            + +L  CA+   L   K +H  I+  GFD                                DL +  + +L++ Y K G    A  +   + +RDVV+WTA+  G    G   + ++L+ SM   G  PN + L   L   S    L  G  +HG  VK G +  V V ++L+ +YAK G +  A R F L   +++ VSW +++   A  G A + L LF+ M    LR    T   V  +CT           H+ ++  G ++       D+ +       C++DL+ + GL ++  +  +++  EP++V W +++
Sbjct:  283 ASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFSACT-----------HAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLL 590          

HSP 7 Score: 65.0846 bits (157), Expect = 3.800e-9
Identity = 51/199 (25.63%), Postives = 93/199 (46.73%), Query Frame = -2

Query: 2914 AKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMP-ERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSS-LVDLYAKCGQMELAERVFLFMD-KKNVVSWNALLNGYALKG--DAGKILNLFQGMTESELRCSKFTLSNVLKSC 3495
             K +HG  +K G    + + N+L+  YAK G    A +  D +  ERD VSWT++   L   G+  + L+L+ +M  +G+ P+    V    AC+ +  +  G         V  ++      + +VDL+ + G ++ A+     M  + +VV+W +LL+   +    D GK+      + E E   +   L+N+  +C
Sbjct:  432 GKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFSACTHAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLLSACRVHKNIDLGKVAAERLLLLEPENSGAYSALANLYSAC 630          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP210_ARATH (Pentatricopeptide repeat-containing protein At3g03580 OS=Arabidopsis thaliana GN=PCMP-H23 PE=2 SV=1)

HSP 1 Score: 427.172 bits (1097), Expect = 1.723e-129
Identity = 254/867 (29.30%), Postives = 452/867 (52.13%), Query Frame = -2

Query:  925 ASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRM-PERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLF-HHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQD 3519
            +S  +L+  + +H  +I  G D        L++ Y+       +  V  R+ P ++V  W ++       G   + L+ Y  +++  V P+ +   + +KAC+   D   G L++ + + +G   D+FVG++LVD+Y++ G +  A +VF  M  +++VSWN+L++GY+  G   + L ++  +  S +    FT+S+VL +   L+ +  G   H   +K+G      V   L+ +Y K     D  +VF  +   + V+++ MI    +   ++E+  +F L       P+  T +S+     +L D    + ++  + K GF  E  + N LI +Y K   +     VF  M   ++ SWNS++SG            +F  M+    + D  T+  ++   + L ++KFG  +H++ IK+G+  +  V  +LID+YAK G + D+  +F+ +   D  +W  +I+   +       ++    M++  V P+  T    L  C+++A    G+ +H   ++ G   ++ + +++++MY+ CGC+E +   F  M   D V+W  M++ Y  + +G K LETF  M   G+ PD V FIA++ ACS+ G V+EG   F+ +   + I P I+H AC++D+L R+ K ++ E FI+ M +  +  IW +VL  CR  G+ +  E  + ++ ELNP    + IL +N +AA  +W+ V  +R  +    + K PG SW+ V   VH+F S D   P+   IY  L+ L       GY+P    +  ++ ++E+  +L   H+ERLA+AF L++  P   +++ KNL +C DCH+  KL+S ++ +EI+VRDAN FH FK G CSC+D
Sbjct:   15 SSSSNLNELRRIHALVISLGLDSSDFFSGKLIDKYSHFREPASSLSVFRRVSPAKNVYLWNSIIRAFSKNGLFPEALEFYGKLRESKVSPDKYTFPSVIKACAGLFDAEMGDLVYEQILDMGFESDLFVGNALVDMYSRMGLLTRARQVFDEMPVRDLVSWNSLISGYSSHGYYEEALEIYHELKNSWIVPDSFTVSSVLPAFGNLLVVKQGQGLHGFALKSGVNSVVVVNNGLVAMYLKFRRPTDARRVFDEMDVRDSVSYNTMICGYLKLEMVEESVRMF-LENLDQFKPDLLTVSSVLRACGHLRDLSLAKYIYNYMLKAGFVLESTVRNILIDVYAKCGDMITARDVFNSMECKDTVSWNSIISGYIQSGDLMEAMKLFKMMMIMEEQADHITYLMLISVSTRLADLKFGKGLHSNGIKSGICIDLSVSNALIDMYAKCGEVGDSLKIFSSMGTGDTVTWNTVISACVRFGDFATGLQVTTQMRKSEVVPDMATFLVTLPMCASLAAKRLGKEIHCCLLRFGYESELQIGNALIEMYSKCGCLENSSRVFERMSRRDVVTWTGMIYAYGMYGEGEKALETFADMEKSGIVPDSVVFIAIIYACSHSGLVDEGLACFEKMKTHYKIDPMIEHYACVVDLLSRSQKISKAEEFIQAMPIKPDASIWASVLRACRTSGDMETAERVSRRIIELNPDDPGYSILASNAYAALRKWDKVSLIRKSLKDKHITKNPGYSWIEVGKNVHVFSSGDDSAPQSEAIYKSLEILYSLMAKEGYIPDPREVSQNLEEEEEKRRLICGHSERLAIAFGLLNTEPGTPLQVMKNLRVCGDCHEVTKLISKIVGREILVRDANRFHLFKDGTCSCKD 880          

HSP 2 Score: 226.483 bits (576), Expect = 9.529e-60
Identity = 145/522 (27.78%), Postives = 272/522 (52.11%), Query Frame = -2

Query: 1978 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG 3543
            +  ++  CA          V+ +I+  GF+ DL + N+LV+ Y++ G+   A +V D MP RD+VSW +L SG  + GY  + L++Y  +K   ++P+   + + L A    L +  G  LHG  +K GV   V V + LV +Y K  +   A RVF  MD ++ VS+N ++ GY       + + +F    + + +    T+S+VL++C +L +L+     ++ ++K G   +  V   L+D+Y+KCG       VF  ++  + V+W+++I    + G + EA +LF +M       +  T+  +  V+  L D  + +G+H+   K G   ++ +SNALI MY K   V +  ++F  M + ++ +WN+++S          G  +  +M      PD+ TF   L  C++L   + G ++H  +++ G +    +G +LI++Y+K G L ++  +F ++  RD+ +WT +I  Y      EKA++ F  M++ G+ P++      + +CS+  +++ G
Sbjct:  109 FPSVIKACAGLFDAEMGDLVYEQILDMGFESDLFVGNALVDMYSRMGLLTRARQVFDEMPVRDLVSWNSLISGYSSHGYYEEALEIYHELKNSWIVPDSFTVSSVLPAFGNLLVVKQGQGLHGFALKSGVNSVVVVNNGLVAMYLKFRRPTDARRVFDEMDVRDSVSYNTMICGYLKLEMVEESVRMFLENLD-QFKPDLLTVSSVLRACGHLRDLSLAKYIYNYMLKAGFVLESTVRNILIDVYAKCGDMITARDVFNSMECKDTVSWNSIISGYIQSGDLMEAMKLFKMMMIMEEQADHITYLMLISVSTRLADLKFGKGLHSNGIKSGICIDLSVSNALIDMYAKCGEVGDSLKIFSSMGTGDTVTWNTVISACVRFGDFATGLQVTTQMRKSEVVPDMATFLVTLPMCASLAAKRLGKEIHCCLLRFGYESELQIGNALIEMYSKCGCLENSSRVFERMSRRDVVTWTGMIYAYGMYGEGEKALETFADMEKSGIVPDSVVFIAIIYACSHSGLVDEG 629          

HSP 3 Score: 98.9821 bits (245), Expect = 1.621e-19
Identity = 55/218 (25.23%), Postives = 112/218 (51.38%), Query Frame = -2

Query: 1672 ILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIE-RDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGK 2322
            I R+ S+  N+    ++HA +I  GL  ++     LID Y+     A +  +F ++   ++++ W ++I  ++++    +A++ +  ++   V+P+ +T  + + +C+ +   E G L++   +  G   D+FV +++VDMY+  G +  A   F  M   D VSWN ++ GY  H    + LE +  + +  + PD  T  +VL A   +  V +G+
Sbjct:   10 ISRALSSSSNLNELRRIHALVISLGLDSSDFFSGKLIDKYSHFREPASSLSVFRRVSPAKNVYLWNSIIRAFSKNGLFPEALEFYGKLRESKVSPDKYTFPSVIKACAGLFDAEMGDLVYEQILDMGFESDLFVGNALVDMYSRMGLLTRARQVFDEMPVRDLVSWNSLISGYSSHGYYEEALEIYHELKNSWIVPDSFTVSSVLPAFGNLLVVKQGQ 227          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: B8AB74_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_02714 PE=4 SV=1)

HSP 1 Score: 667.537 bits (1721), Expect = 0.000e+0
Identity = 349/785 (44.46%), Postives = 493/785 (62.80%), Query Frame = -2

Query: 1261 NPSLKVPLFKEKTKLE-GAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGC 3612
            +PS   P  ++  +L   A+ L+ Y+  L  CA+  +L   KA+H ++++ G  PD  L +SL+N Y K G    A  V D MP RDVV+WTA+ S +   G     L L+  M ++GV+PNG  L  ALKAC++  DL F   +H + VK+  L D +V SSLV+ Y  CG++++AER  L    ++ VSWNALLN YA  GD  K++ +F  + ES    SK+TL  VLK C  L     G   H LVIK G E D  +  CL+++YSKC  AED  +VF RI EP++V  S MI C D      EA ++F  M   GV PNQ+TF  +A VA+  GD   C  +HA I K GF     + +A++ MY+K  +VQ+    F  M   + ASWN+LLSG  +G  C+ G  IF +++ EG   +  T+  ILR C++L++++FG QVHA ++K+G + +  V   L+D+Y + G   +A L+F +L ERD+FSWT +++ YA++D  EKAI+CF  M R+   PN+ TLAT LS CS++A L +G  LHS  IK+G +  V VSS++VDMY  CG + +AE  F    + D V WN ++ GY QH  G K LE F+ M+D+G  PDE+TF+ VLSACS+ G ++EG+ YF  L++V+GI PT++H ACM+DIL +AGK  E ES I +M +T +  +W+T+LG CRMHGN ++ E AAEKLFE  P   S  ILL+NI+A   RW DV ++R+++   GVKKEPGCSW+ +NG++H+F S DGC
Sbjct:   39 HPSAPSPSPRDDERLRLRAEELRSYAAALQGCAASRALRRGKALHARLLRSGPRPDAFLHDSLLNMYCKCGRLADARSVFDGMPHRDVVAWTAMVSAITAAGDAGAALRLFAEMSEEGVVPNGFALAAALKACTVGSDLGFTPQVHAQAVKLEGLFDPYVSSSLVEAYVSCGEVDVAERALLDSPVRSDVSWNALLNEYARDGDYAKVMLVFDKLVESGDEISKYTLPTVLKCCMELGLAKSGQAVHGLVIKRGLETDRVLNNCLIEMYSKCLSAEDAYEVFARIDEPDVVHCSLMISCFDRHDMAPEAFDIFMQMSDMGVKPNQYTFVGLAIVASRTGDVNLCRSIHAHIVKSGFSRTKGVCDAIVGMYVKTGAVQDAILAFDLMQGPDIASWNTLLSGFYSGNNCEHGLRIFKELICEGVLANKYTYVGILRCCTSLMDLRFGCQVHACVLKSGFQGDYDVSKMLLDMYVQAGCFTNARLVFDRLKERDVFSWTVVMSTYAKTDEGEKAIECFRSMLRENKRPNDATLATSLSVCSDLACLGSGLQLHSYTIKSGWNSSV-VSSALVDMYVKCGNLADAEMLFDESDTHDLVEWNTIICGYAQHGHGYKALEAFQEMIDEGNVPDEITFVGVLSACSHAGLLDEGRRYFKLLSSVYGITPTLEHYACMVDILAKAGKLAEAESLINEMPLTPDASLWKTILGACRMHGNIEIAERAAEKLFESQPDDISSCILLSNIYADLKRWNDVAKLRSMLVDRGVKKEPGCSWIEINGKLHVFLSQDGC 822          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A0E0FP70_ORYNI (Uncharacterized protein OS=Oryza nivara PE=4 SV=1)

HSP 1 Score: 667.537 bits (1721), Expect = 0.000e+0
Identity = 349/785 (44.46%), Postives = 493/785 (62.80%), Query Frame = -2

Query: 1261 NPSLKVPLFKEKTKLE-GAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGC 3612
            +PS   P  ++  +L   A+ L+ Y+  L  CA+  +L   KA+H ++++ G  PD  L +SL+N Y K G    A  V D MP RDVV+WTA+ S +   G     L L+  M ++GV+PNG  L  ALKAC++  DL F   +H + VK+  L D +V SSLV+ Y  CG++++AER  L    ++ VSWNALLN YA  GD  K++ +F  + ES    SK+TL  VLK C  L     G   H LVIK G E D  +  CL+++YSKC  AED  +VF RI EP++V  S MI C D      EA ++F  M   GV PNQ+TF  +A VA+  GD   C  +HA I K GF     + +A++ MY+K  +VQ+    F  M   + ASWN+LLSG  +G  C+ G  IF +++ EG   +  T+  ILR C++L++++FG QVHA ++K+G + +  V   L+D+Y + G   +A L+F +L ERD+FSWT +++ YA++D  EKAI+CF  M R+   PN+ TLAT LS CS++A L +G  LHS  IK+G +  V VSS++VDMY  CG + +AE  F    + D V WN ++ GY QH  G K LE F+ M+D+G  PDE+TF+ VLSACS+ G ++EG+ YF  L++V+GI PT++H ACM+DIL +AGK  E ES I +M +T +  +W+T+LG CRMHGN ++ E AAEKLFE  P   S  ILL+NI+A   RW DV ++R+++   GVKKEPGCSW+ +NG++H+F S DGC
Sbjct:   39 HPSAPSPSPRDDERLRLRAEELRSYAAALQGCAASRALRRGKALHARLLRSGPRPDAFLHDSLLNMYCKCGRLADARSVFDGMPHRDVVAWTAMVSAITAAGDAGAALRLFAEMSEEGVVPNGFALAAALKACTVGSDLGFTPQVHAQAVKLEGLFDPYVSSSLVEAYVSCGEVDVAERALLDSPVRSDVSWNALLNEYARDGDYAKVMLVFDKLVESGDEISKYTLPTVLKCCMELGLAKSGQAVHGLVIKRGLETDRVLNNCLIEMYSKCLSAEDAYEVFARIDEPDVVHCSLMISCFDRHDMAPEAFDIFMQMSDMGVKPNQYTFVGLAIVASRTGDVNLCRSIHAHIVKSGFSRTKGVCDAIVGMYVKTGAVQDAILAFDLMQGPDIASWNTLLSGFYSGNNCEHGLRIFKELICEGVLANKYTYVGILRCCTSLMDLRFGCQVHACVLKSGFQGDYDVSKMLLDMYVQAGCFTNARLVFDRLKERDVFSWTVVMSTYAKTDEGEKAIECFRSMLRENKRPNDATLATSLSVCSDLACLGSGLQLHSYTIKSGWNSSV-VSSALVDMYVKCGNLADAEMLFDESDTHDLVEWNTIICGYAQHGHGYKALEAFQEMIDEGNVPDEITFVGVLSACSHAGLLDEGRRYFKLLSSVYGITPTLEHYACMVDILAKAGKLAEAESLINEMPLTPDASLWKTILGACRMHGNIEIAERAAEKLFESQPDDISSCILLSNIYADLKRWNDVAKLRSMLVDRGVKKEPGCSWIEINGKLHVFLSQDGC 822          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A0E0MYW2_ORYRU (Uncharacterized protein OS=Oryza rufipogon PE=4 SV=1)

HSP 1 Score: 667.152 bits (1720), Expect = 0.000e+0
Identity = 350/785 (44.59%), Postives = 494/785 (62.93%), Query Frame = -2

Query: 1261 NPSLKVPLFKEKTKLE-GAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGC 3612
            +PS   P  ++  +L   A+ L+ Y+  L  CA+  +L   KA+H ++++ G  PD  L +SL+N Y K G    A  V D MP RDVV+WTA+ S +   G     L L+  M ++GV+PNG  L  ALKAC++  DL F   +H + VK+  LLD +V SSLV+ Y  CG++++AER  L    ++ VSWNALLN YA  GD  K++ +F  + ES    SK+TL  VLK C  L     G   H LVIK G E D  +  CL+++YSKC  AED  +VF RI EP++V  S MI C D      EA ++F  M   GV PNQ+TF  +A VA+  GD   C  +HA I K GF     + +A++ MY+K  +VQ+    F  M   + ASWN+LLSG  +G  C+ G  IF +++ EG   +  T+  ILR C++L++++FG QVHA ++K+G + +  V   L+D+Y + G   +A L+F +L ERD+FSWT +++ YA++D  EKAI+CF  M R+   PN+ TLAT LS CS++A L +G  LHS  IK+G +  V VSS++VDMY  CG + +AE  F    + D V WN ++ GY QH  G K LE F+ M+D+G  PDE+TF+ VLSACS+ G ++EG+ YF  L++V+GI PT++H ACM+DIL +AGK  E ES I +M +T +  +W+T+LG CRMHGN ++ E AAEKLFE  P   S  ILL+NI+A   RW DV ++R+++   GVKKEPGCSW+ +NG++H+F S DGC
Sbjct:   39 HPSAPSPSPRDDERLRLRAEELRSYAAALQGCAASRALRRGKALHARLLRSGPRPDAFLHDSLLNMYCKCGRLADARSVFDGMPHRDVVAWTAMVSAITAAGDAGAALRLFAEMSEEGVVPNGFALAAALKACTVGSDLGFTPQVHAQAVKLEGLLDPYVSSSLVEAYVSCGEVDVAERALLDSPVRSDVSWNALLNEYARDGDYAKVMLVFDKLVESGDEISKYTLPTVLKCCMELGLAKSGQAVHGLVIKRGLETDRVLNNCLIEMYSKCLSAEDAYEVFARIDEPDVVHCSLMISCFDRHDMAPEAFDIFMQMSDMGVKPNQYTFVGLAIVASITGDVNLCRSIHAHIVKSGFSRTKGVCDAIVGMYVKTGAVQDAILAFDLMQGPDIASWNTLLSGFYSGNNCEHGLRIFKELICEGVLANKYTYVGILRCCTSLMDLRFGCQVHACVLKSGFQGDYDVSKMLLDMYVQAGCFTNARLVFDRLKERDVFSWTVVMSTYAKTDEGEKAIECFRSMLRENKRPNDATLATSLSVCSDLACLGSGLQLHSYTIKSGWNSSV-VSSALVDMYVKCGNLADAEMLFDESDTHDLVEWNTIICGYAQHGHGYKALEAFQEMIDEGNVPDEITFVGVLSACSHAGLLDEGRRYFKLLSSVYGITPTLEHYACMVDILAKAGKLAEAESLINEMPLTPDASLWKTILGACRMHGNIEIAERAAEKLFESQPDDISSCILLSNIYADLKRWNDVAKLRSMLVDRGVKKEPGCSWIEINGKLHVFLSQDGC 822          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A0D9YBC5_9ORYZ (Uncharacterized protein OS=Oryza glumipatula PE=4 SV=1)

HSP 1 Score: 667.152 bits (1720), Expect = 0.000e+0
Identity = 350/785 (44.59%), Postives = 494/785 (62.93%), Query Frame = -2

Query: 1261 NPSLKVPLFKEKTKLE-GAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGC 3612
            +PS   P  ++  +L   A+ L+ Y+  L  CA+  +L   KA+H ++++ G  PD  L +SL+N Y K G    A  V D MP RDVV+WTA+ S +   G     L L+  M ++GV+PNG  L  ALKAC++  DL F   +H + VK+  LLD +V SSLV+ Y  CG++++AER  L    ++ VSWNALLN YA  GD  K++ +F  + ES    SK+TL  VLK C  L     G   H LVIK G E D  +  CL+++YSKC  AED  +VF RI EP++V  S MI C D      EA ++F  M   GV PNQ+TF  +A VA+  GD   C  +HA I K GF     + +A++ MY+K  +VQ+    F  M   + ASWN+LLSG  +G  C+ G  IF +++ EG   +  T+  ILR C++L++++FG QVHA ++K+G + +  V   L+D+Y + G   +A L+F +L ERD+FSWT +++ YA++D  EKAI+CF  M R+   PN+ TLAT LS CS++A L +G  LHS  IK+G +  V VSS++VDMY  CG + +AE  F    + D V WN ++ GY QH  G K LE F+ M+D+G  PDE+TF+ VLSACS+ G ++EG+ YF  L++V+GI PT++H ACM+DIL +AGK  E ES I +M +T +  +W+T+LG CRMHGN ++ E AAEKLFE  P   S  ILL+NI+A   RW DV ++R+++   GVKKEPGCSW+ +NG++H+F S DGC
Sbjct:   39 HPSAPSPSPRDDERLRLRAEELRSYAAALQGCAASRALLRGKALHARLLRSGPRPDAFLHDSLLNMYCKCGRLADARSVFDGMPHRDVVAWTAMVSAITAAGDAGAALRLFAEMSEEGVVPNGFALAAALKACTVGSDLGFTPQVHAQAVKLEGLLDPYVSSSLVEAYVSCGEVDVAERALLDSPVRSDVSWNALLNEYARDGDYAKVMLVFDKLVESGDEISKYTLPTVLKCCMELGLAKSGQAVHGLVIKRGLETDRVLNNCLIEMYSKCLSAEDAYEVFARIDEPDVVHCSLMISCFDRHDMAPEAFDIFMQMSDMGVKPNQYTFVGLAIVASRTGDVNLCRSIHAPIVKSGFSRTKGVCDAIVGMYVKTGAVQDAILAFDLMQGPDIASWNTLLSGFYSGNNCEHGLRIFKELICEGVLANKYTYVGILRCCTSLMDLRFGCQVHACVLKSGFQGDYDVSKMLLDMYVQAGCFTNARLVFDRLKERDVFSWTVVMSTYAKTDEGEKAIECFRSMLRENKRPNDATLATSLSVCSDLACLGSGLQLHSYTIKSGWNSSV-VSSALVDMYVKCGNLADAEMLFDESDTHDLVEWNTIICGYAQHGHGYKALEAFQEMIDEGNVPDEITFVCVLSACSHAGLLDEGRMYFKLLSSVYGITPTLEHYACMVDILAKAGKLAEAESLINEMPLTPDASLWKTILGACRMHGNIEIAERAAEKLFESQPDDISSCILLSNIYADLKRWNDVAKLRSMLVDRGVKKEPGCSWIEINGKLHVFLSQDGC 822          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: T1MAF5_TRIUA (Uncharacterized protein OS=Triticum urartu PE=4 SV=1)

HSP 1 Score: 655.596 bits (1690), Expect = 0.000e+0
Identity = 341/783 (43.55%), Postives = 492/783 (62.84%), Query Frame = -2

Query: 1249 PLFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACS-LSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKH 3594
            P       L  A  L+ ++  L DCA + +L   + +H ++++    PD  L +SL+N Y K G    A +V D MP RDVV+WTAL S     G   + LDL+C M + G+ PNG  L + LKACS +S    F   +HG+ VK+  L D +VGSSLV  YA  G+++ AE V L + +++ +SWNALL  YA +GD  K +++F  ++E     SK+T+  +LK C  L     G   H+LV+K G E D  +  CL+++YS+C  AE+  +VF RI EP++V  SAMI      G   EA +LF  M   GV PNQ+TF  IAGVA+  GD   C   HA + K G     +++++++ MY+K+ + Q+    F  M   ++ SWN+ LSG  +G  C++G  IF +M  EGF  +  T+  +LR C++L+N+ +G QVHA ++K+GL+ +N V   L+D+YA++G    A L+F +L ERD FSWT +++GYA++D +EK ++CF  M ++   PN+ TLA  LS  S+MA L +G  LHS AIK+G +  V VS ++VDMY  CG I +AE  F    + D V+WN ++ GY QH  G K L+TFR M+D G  PD++TF+ VLSACS+ G ++EG++YF  L++V+GI PT++H ACMIDIL +AG+  E ES I  M +  +  IW T+LG CR+HGN ++ E AAE+L EL+P   S  +LL+NI+A  GRW DV R+R ++   GVKKEPGCSW+ VNGQ+H+F S DGC PK+
Sbjct:   24 PSHPNSRPLPEADELRSHAAALQDCAGRPALRCGQELHARLLRSARQPDTFLLDSLLNMYCKCGRLADARRVFDGMPHRDVVAWTALLSAYAAAGDAEEALDLFCQMNQQGLAPNGFTLASVLKACSVMSSHSEFTRQVHGQVVKLQGLDDPYVGSSLVQAYAIHGEVDAAETVLLGLPERSDMSWNALLTEYARQGDYRKAMHVFHKLSEFGDEISKYTVPTLLKCCVELGLAKSGQALHALVVKRGLETDNVLNNCLVEMYSRCLSAEEAYQVFIRIDEPDVVHCSAMISSFGRHGMAWEAFDLFVKMSDMGVKPNQYTFVGIAGVASKTGDANLCRCAHAYVVKSGLAMPKLVADSILNMYVKVGAAQDATVAFHLMHEPDTFSWNTFLSGFYSGSNCEQGLRIFKQMKCEGFSANKYTYVGVLRCCTSLMNLMYGTQVHACVLKSGLQSDNDVSRMLLDMYAQSGCFTSACLVFDRLEERDAFSWTVIMSGYAKTDEAEKVMECFRSMLQENKRPNDATLAVSLSVSSDMASLGSGLQLHSWAIKSGWNSSV-VSGALVDMYVKCGNITDAEMLFYESETCDQVAWNTLICGYSQHGHGYKALDTFRRMVDDGKRPDDITFVGVLSACSHAGLLDEGRKYFQLLSSVYGITPTMEHYACMIDILSKAGRLAEAESLINQMPLIPDSSIWRTILGACRIHGNIEIAERAAERLLELDPQDVSSSVLLSNIYADLGRWSDVTRLRNMLLDHGVKKEPGCSWIEVNGQIHVFLSQDGC-PKY 804