g.barbadense_cottongen_reftransV1_0091917, g.barbadense_cottongen_reftransV1_0091917 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP249_ARATH (Pentatricopeptide repeat-containing protein At3g22690 OS=Arabidopsis thaliana GN=PCMP-H56 PE=2 SV=1)

HSP 1 Score: 429.869 bits (1104), Expect = 5.378e-131
Identity = 259/808 (32.05%), Postives = 411/808 (50.87%), Query Frame = -2

Query:  922 TALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQME---LAERVFLFMDKKNV-VSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELF-SLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAK-------------------------------NGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQ-GVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3234
            ++LK C    +L    + H    K G+  DV   + LV    + G  E    A+ VF   +       +N+L+ GYA  G   + + LF  M  S +   K+T    L +C        G+  H L++K G   D FV   L+  Y++CG  +   KVF  + E N+V+W++MI     +   ++A +LF  ++R + V+PN  T   +    A L D    E V+A I   G E   ++ +AL+ MYMK  ++    R+F E  + N    N++ S             +F+ M+  G +PD  +  S + SCS L NI +G   H ++++NG +  + +  +LID+Y K                               NG +  A   F  + E+++ SW  +I+G  Q    E+AI+ F  MQ Q GV  +  T+ +  S+C ++  L+  + ++    K G   DV + +++VDM++ CG  E A + F  + + D  +W   +          + +E F  M+++GL+PD V F+  L+ACS+ G V +GKE F S+  + G+ P   H  CM+D+LGRAG   E    I+DM +  N +IW ++L  CR+ GN ++   AAEK+  L P     Y+LL+N++A+ GRW D+ +VR  M   G++K PG S + + G+ H F S D  HP+   I   L E+ +++   G+VP   ++L DV + EK+  L  H+E+LA+A+ LIS N   TIRI KNL +C DCH F K  S V  +EI++RD N FH+ + G CSC D+
Sbjct:   37 SSLKNCKTIDEL---KMFHRSLTKQGLDNDVSTITKLVARSCELGTRESLSFAKEVFENSESYGTCFMYNSLIRGYASSGLCNEAILLFLRMMNSGISPDKYTFPFGLSACAKSRAKGNGIQIHGLIVKMGYAKDLFVQNSLVHFYAECGELDSARKVFDEMSERNVVSWTSMICGYARRDFAKDAVDLFFRMVRDEEVTPNSVTMVCVISACAKLEDLETGEKVYAFIRNSGIEVNDLMVSALVDMYMKCNAIDVAKRLFDEYGASNLDLCNAMASNYVRQGLTREALGVFNLMMDSGVRPDRISMLSAISSCSQLRNILWGKSCHGYVLRNGFESWDNICNALIDMYMKCHRQDTAFRIFDRMSNKTVVTWNSIVAGYVENGEVDAAWETFETMPEKNIVSWNTIISGLVQGSLFEEAIEVFCSMQSQEGVNADGVTMMSIASACGHLGALDLAKWIYYYIEKNGIQLDVRLGTTLVDMFSRCGDPESAMSIFNSLTNRDVSAWTAAIGAMAMAGNAERAIELFDDMIEQGLKPDGVAFVGALTACSHGGLVQQGKEIFYSMLKLHGVSPEDVHYGCMVDLLGRAGLLEEAVQLIEDMPMEPNDVIWNSLLAACRVQGNVEMAAYAAEKIQVLAPERTGSYVLLSNVYASAGRWNDMAKVRLSMKEKGLRKPPGTSSIQIRGKTHEFTSGDESHPEMPNIEAMLDEVSQRASHLGHVPDLSNVLMDVDEKEKIFMLSRHSEKLAMAYGLISSNKGTTIRIVKNLRVCSDCHSFAKFASKVYNREIILRDNNRFHYIRQGKCSCGDF 841          

HSP 2 Score: 211.46 bits (537), Expect = 4.337e-55
Identity = 186/714 (26.05%), Postives = 323/714 (45.24%), Query Frame = -2

Query: 1543 LEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGV---FGYACKVLDRMPERDV-VSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMT-ESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRS--------------------------VQNG-----WRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLA-EGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMK 3570
            L  +K  K     L +C +   L   K  H  + K+G D D+     LV    + G      +A +V +          + +L  G  + G   + + L+  M   G+ P+ +     L AC+ S     G  +HG  VK+G   D+FV +SLV  YA+CG+++ A +VF  M ++NVVSW +++ GYA +  A   ++LF  M  + E+  +  T+  V+ +C  L +L  G   ++ +  +G E ++ +   L+D+Y KC   +   ++F      N+   +AM      QG  +EA  +F+LM   GV P++ +  S     + L + L+ +  H  + ++GFES   + NALI MYMK                             V+NG     W  F+ M   N  SWN+++SG   G   +    +F  M + EG   D  T  SI  +C +L  +     ++ +I KNG++ +  +GT+L+D++++ G    A  +F  L  RD+ +WTA I   A +  +E+AI+ F+ M  QG+ P+       L++CS+  +++ G ++ +SM    G S +      +VD+    G +EEA                                   +++ D  +EP++V + ++L+AC   G V E   Y      V     T  +   + ++   AG++N++      MK
Sbjct:   26 LNQSKCTKATPSSLKNCKTIDEL---KMFHRSLTKQGLDNDVSTITKLVARSCELGTRESLSFAKEVFENSESYGTCFMYNSLIRGYASSGLCNEAILLFLRMMNSGISPDKYTFPFGLSACAKSRAKGNGIQIHGLIVKMGYAKDLFVQNSLVHFYAECGELDSARKVFDEMSERNVVSWTSMICGYARRDFAKDAVDLFFRMVRDEEVTPNSVTMVCVISACAKLEDLETGEKVYAFIRNSGIEVNDLMVSALVDMYMKCNAIDVAKRLFDEYGASNLDLCNAMASNYVRQGLTREALGVFNLMMDSGVRPDRISMLSAISSCSQLRNILWGKSCHGYVLRNGFESWDNICNALIDMYMKCHRQDTAFRIFDRMSNKTVVTWNSIVAGYVENGEVDAAWETFETMPEKNIVSWNTIISGLVQGSLFEEAIEVFCSMQSQEGVNADGVTMMSIASACGHLGALDLAKWIYYYIEKNGIQLDVRLGTTLVDMFSRCGDPESAMSIFNSLTNRDVSAWTAAIGAMAMAGNAERAIELFDDMIEQGLKPDGVAFVGALTACSHGGLVQQGKEIFYSMLKLHGVSPEDVHYGCMVDLLGRAGLLEEA----------------------------------VQLIEDMPMEPNDVIWNSLLAACRVQGNV-EMAAYAAEKIQVLAPERTGSY-VLLSNVYASAGRWNDMAKVRLSMK 700          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP108_ARATH (Putative pentatricopeptide repeat-containing protein At1g68930 OS=Arabidopsis thaliana GN=PCMP-H22 PE=3 SV=1)

HSP 1 Score: 426.402 bits (1095), Expect = 7.844e-131
Identity = 239/726 (32.92%), Postives = 387/726 (53.31%), Query Frame = -2

Query:  922 TESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDE-----------FVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELF-SLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVF-------------------------------KEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 2970
              ++ R  K    N++++  Y     +  I H+  +     +             F    LL  YSK GL  +    F+++ + + V W+ +I+     G +  A + + ++MR    +  + T  ++  ++++ G     + +H  + K GFES +++ + L+ MY  +  + +  +VF                               + M   + +    +   +QNG         F +M  +G K D   F S+L +C  L  I  G Q+HA II+   +D+  VG++LID+Y K   L  A+ +F ++ ++++ SWTA++ GY Q+  +E+A+K F  MQR G+ P+++TL   +S+C+N++ LE G   H  AI +G    V VS+S+V +Y  CG I+++   F  M   D VSW  M+  Y Q  + ++ ++ F  M+  GL+PD VT   V+SACS  G V +G+ YF  +T+ +GIVP+I H +CMID+  R+G+  E   FI  M    + + W T+L  CR  GN ++G+ AAE L EL+P   + Y LL++I+A+KG+W+ V ++R  M    VKKEPG SW+   G++H F + D   P   +IY KL+EL  K I  GY P T  + +DV +  K++ L +H+ERLA+AF LI +   + IR+ KNL +C DCH+  K +S V  +EI+VRDA  FH FK G CSC D+
Sbjct:   18 ARNQSRYVKMIHGNIIRALPYPETFLYNNIVHAYALMKSSTYARRVFDRIPQPNLFSWNNLLLAYSKAGLISEMESTFEKLPDRDGVTWNVLIEGYSLSGLVGAAVKAYNTMMRDFSANLTRVTLMTMLKLSSSNGHVSLGKQIHGQVIKLGFESYLLVGSPLLYMYANVGCISDAKKVFYGLDDRNTVMYNSLMGGLLACGMIEDALQLFRGMEKDSVSWAAMIKGLAQNG-LAKEAIECFREMKVQGLKMDQYPFGSVLPACGGLGAINEGKQIHACIIRTNFQDHIYVGSALIDMYCKCKCLHYAKTVFDRMKQKNVVSWTAMVVGYGQTGRAEEAVKIFLDMQRSGIDPDHYTLGQAISACANVSSLEEGSQFHGKAITSGLIHYVTVSNSLVTLYGKCGDIDDSTRLFNEMNVRDAVSWTAMVSAYAQFGRAVETIQLFDKMVQHGLKPDGVTLTGVISACSRAGLVEKGQRYFKLMTSEYGIVPSIGHYSCMIDLFSRSGRLEEAMRFINGMPFPPDAIGWTTLLSACRNKGNLEIGKWAAESLIELDPHHPAGYTLLSSIYASKGKWDSVAQLRRGMREKNVKKEPGQSWIKWKGKLHSFSADDESSPYLDQIYAKLEELNNKIIDNGYKPDTSFVHHDVEEAVKVKMLNYHSERLAIAFGLIFVPSGQPIRVGKNLRVCVDCHNATKHISSVTGREILVRDAVRFHRFKDGTCSCGDF 742          

HSP 2 Score: 181.415 bits (459), Expect = 9.555e-46
Identity = 132/511 (25.83%), Postives = 238/511 (46.58%), Query Frame = -2

Query: 2116 LYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKD-GVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVV------------------------------SWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKN-GLKDNNLVGTSLIDLYAKNGFLADAELLFTQL-IERDLFSWTALIA 3546
            LY+ I+H  A   S + A+ V  +I +    P+L  WN+L+ AY+K G+        +++P+RD V+W  L  G    G     +  Y +M +D         L+T LK  S +  +  G  +HG+ +K+G    + VGS L+ +YA  G +  A++VF  +D +N V                              SW A++ G A  G A + +  F+ M    L+  ++   +VL +C  L  +  G   H+ +I+T  +   +VG  L+D+Y KC        VF R+++ N+V+W+AM+    + G+ +EA ++F  M+  G+ P+ +T        AN+         H      G    + +SN+L+ +Y K   + +  R+F EM+  ++ S    + + +Q G+  +    +F KM+  G KPD  T T ++ +CS    ++ G +    +    G+  +    + +IDL++++G L +A      +    D   WT L++
Sbjct:   43 LYNNIVHAYALMKSSTYARRVFDRIPQ----PNLFSWNNLLLAYSKAGLISEMESTFEKLPDRDGVTWNVLIEGYSLSGLVGAAVKAYNTMMRDFSANLTRVTLMTMLKLSSSNGHVSLGKQIHGQVIKLGFESYLLVGSPLLYMYANVGCISDAKKVFYGLDDRNTVMYNSLMGGLLACGMIEDALQLFRGMEKDSVSWAAMIKGLAQNGLAKEAIECFREMKVQGLKMDQYPFGSVLPACGGLGAINEGKQIHACIIRTNFQDHIYVGSALIDMYCKCKCLHYAKTVFDRMKQKNVVSWTAMVVGYGQTGRAEEAVKIFLDMQRSGIDPDHYTLGQAISACANVSSLEEGSQFHGKAITSGLIHYVTVSNSLVTLYGKCGDIDDSTRLFNEMNVRDAVSWTAMVSAYAQFGRAVET-IQLFDKMVQHGLKPDGVTLTGVISACSRAGLVEKGQRYFKLMTSEYGIVPSIGHYSCMIDLFSRSGRLEEAMRFINGMPFPPDAIGWTTLLS 548          

HSP 3 Score: 143.665 bits (361), Expect = 1.439e-33
Identity = 84/305 (27.54%), Postives = 159/305 (52.13%), Query Frame = -2

Query: 2695 FKEKTKLEGAKRLKL-YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWG-----LIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCLDEQGQIQ 3588
            F+E  K++G K  +  +  +L  C   G+++  K +H  II+  F   +++ ++L++ Y K     YA  V DRM +++VVSWTA+  G    G   + + ++  M++ G+ P+ + L  A+ AC+    L  G+  HG+ +  G++  V V +SLV LY KCG ++ + R+F  M+ ++ VSW A+++ YA  G A + + LF  M +  L+    TL+ V+ +C+    +  G     L+     I     H      C++DL+S+ G  E+ ++    +   P+ + W+ ++     +G ++
Sbjct:  258 FRE-MKVQGLKMDQYPFGSVLPACGGLGAINEGKQIHACIIRTNFQDHIYVGSALIDMYCKCKCLHYAKTVFDRMKQKNVVSWTAMVVGYGQTGRAEEAVKIFLDMQRSGIDPDHYTLGQAISACANVSSLEEGSQFHGKAITSGLIHYVTVSNSLVTLYGKCGDIDDSTRLFNEMNVRDAVSWTAMVSAYAQFGRAVETIQLFDKMVQHGLKPDGVTLTGVISACSRAGLVEKGQRYFKLMTSEYGIVPSIGHYS----CMIDLFSRSGRLEEAMRFINGMPFPPDAIGWTTLLSACRNKGNLE 557          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PPR45_ARATH (Pentatricopeptide repeat-containing protein At1g15510, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H73 PE=3 SV=1)

HSP 1 Score: 428.713 bits (1101), Expect = 3.197e-130
Identity = 263/803 (32.75%), Postives = 424/803 (52.80%), Query Frame = -2

Query:  925 GLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTE-SELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSD-CSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQD 3327
            GL   G   + + L  SM++  V  +    V  ++ C        G+ ++   +     L V +G++ + ++ + G +  A  VF  M ++N+ SWN L+ GYA +G   + + L+  M     ++   +T   VL++C  + +L  G   H  V++ G E D  V   L+ +Y KCG  +    +F R+   +I++W+AMI    E G   E  ELF  MR   V P+  T  S+      LGDR     +HA +   GF  +I + N+L  MY+   S +   ++F  M   +  SW +++SG +     D+    +  M  +  KPD  T  ++L +C+ L ++  G ++H   IK  L    +V  +LI++Y+K   +  A  +F  +  +++ SWT++IAG   ++ C E  I  F    +  + PN  TL   L++C+ +  L  G+ +H+  ++ G   D F+ ++++DMY  CG +  A + F      D  SWN++L GY +  QG  V+E F  M+   + PDE+TFI++L  CS    V +G  YF  + + +G+ P +KH AC++D+LGRAG+  E   FI+ M VT +P +W  +L  CR+H    LGE +A+ +FEL+     +YILL N++A  G+W +V +VR +M   G+  + GCSWV V G+VH F S D  HP+  EI   L+   EK    G    ++    D ++  + E    H+ER A+AF LI+  P   I + KNL +CE+CHD +K +S  +++EI VRDA  FHHFK G CSC D
Sbjct:   68 GLCANGKLEEAMKLLNSMQELRVAVDEDVFVALVRLCEWKRAQEEGSKVYSIALSSMSSLGVELGNAFLAMFVRFGNLVDAWYVFGKMSERNLFSWNVLVGGYAKQGYFDEAMCLYHRMLWVGGVKPDVYTFPCVLRTCGGIPDLARGKEVHVHVVRYGYELDIDVVNALITMYVKCGDVKSARLLFDRMPRRDIISWNAMISGYFENGMCHEGLELFFAMRGLSVDPDLMTLTSVISACELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVSWTTMISGYEYNFLPDKAIDTYRMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAIKARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEALI--FLRQMKMTLQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDFLPNALLDMYVRCGRMNTAWSQFNSQ-KKDVTSWNILLTGYSERGQGSMVVELFDRMVKSRVRPDEITFISLLCGCSKSQMVRQGLMYFSKMED-YGVTPNLKHYACVVDLLGRAGELQEAHKFIQKMPVTPDPAVWGALLNACRIHHKIDLGELSAQHIFELDKKSVGYYILLCNLYADCGKWREVAKVRRMMKENGLTVDAGCSWVEVKGKVHAFLSDDKYHPQTKEINTVLEGFYEKMSEVGLTKISESSSMDETEISRDEIFCGHSERKAIAFGLINTVPGMPIWVTKNLSMCENCHDTVKFISKTVRREISVRDAEHFHHFKDGECSCGD 866          

HSP 2 Score: 201.445 bits (511), Expect = 8.851e-52
Identity = 147/546 (26.92%), Postives = 263/546 (48.17%), Query Frame = -2

Query: 1816 LHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSM-KKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMML 3447
            + L N+ +  + + G    A  V  +M ER++ SW  L  G   +GY  + + LY  M    GV P+ +     L+ C    DL  G  +H   V+ G  LD+ V ++L+ +Y KCG ++ A  +F  M +++++SWNA+++GY   G   + L LF  M    +     TL++V+ +C  L +   G   H+ VI TG   D  V   L  +Y   G   +  K+F R++  +IV+W+ MI   +      +A + + +M    V P++ T A++    A LGD      +H    K    S +I++N LI MY K + +     +F  +   N  SW S+++G +    C     IF + +    +P+  T T+ L +C+ +  +  G ++HAH+++ G+  ++ +  +L+D+Y + G +  A   F    ++D+ SW  L+ GY++       ++ F+ M +  V P+  T  + L  CS   M+  G +  S     G + ++   + +VD+    G ++EA      M V+ D   W  +L
Sbjct:  129 VELGNAFLAMFVRFGNLVDAWYVFGKMSERNLFSWNVLVGGYAKQGYFDEAMCLYHRMLWVGGVKPDVYTFPCVLRTCGGIPDLARGKEVHVHVVRYGYELDIDVVNALITMYVKCGDVKSARLLFDRMPRRDIISWNAMISGYFENGMCHEGLELFFAMRGLSVDPDLMTLTSVISACELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVSWTTMISGYEYNFLPDKAIDTYRMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAIKARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEAL-IFLRQMKMTLQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDFLPNALLDMYVRCGRMNTAWSQFNSQ-KKDVTSWNILLTGYSERGQGSMVVELFDRMVKSRVRPDEITFISLLCGCSKSQMVRQGLMYFSKMEDYGVTPNLKHYACVVDLLGRAGELQEAHKFIQKMPVTPDPAVWGALL 672          

HSP 3 Score: 192.2 bits (487), Expect = 8.449e-49
Identity = 125/473 (26.43%), Postives = 225/473 (47.57%), Query Frame = -2

Query: 2119 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQL-IERDLFSWTALI 3534
            +L  C     L+  K VH  +++ G++ D+ + N+L+  Y K G    A  + DRMP RD++SW A+ SG    G  ++ L+L+ +M+   V P+   L + + AC L  D   G  +H   +  G  +D+ V +SL  +Y   G    AE++F  M++K++VSW  +++GY       K ++ ++ M +  ++  + T++ VL +C  L +L  G+  H L IK        V   L+++YSKC   +  L +F  I   N+++W+++I  L    +  EA      M+   + PN  T  +     A +G  +  + +HA + + G   +  L NAL+ MY++   +   W  F       ++    L   S+ G+       +F +M+    +PD  TF S+L  CS    ++ G    + +   G+  N      ++DL  + G L +A     ++ +  D   W AL+
Sbjct:  202 VLRTCGGIPDLARGKEVHVHVVRYGYELDIDVVNALITMYVKCGDVKSARLLFDRMPRRDIISWNAMISGYFENGMCHEGLELFFAMRGLSVDPDLMTLTSVISACELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVSWTTMISGYEYNFLPDKAIDTYRMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAIKARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEALIFLRQMKMT-LQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDFLPNALLDMYVRCGRMNTAWSQFNSQKKDVTSWNILLTGYSERGQG-SMVVELFDRMVKSRVRPDEITFISLLCGCSKSQMVRQGLMYFSKMEDYGVTPNLKHYACVVDLLGRAGELQEAHKFIQKMPVTPDPAVWGALL 672          

HSP 4 Score: 184.496 bits (467), Expect = 2.498e-46
Identity = 134/498 (26.91%), Postives = 244/498 (49.00%), Query Frame = -2

Query: 1543 LNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELF-SLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKE-YFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMK 3030
            L+G    G   + + L   M E  +   +     +++ C +      G   +S+ + +       +G   L ++ + G   D   VF ++ E N+ +W+ ++    +QG   EA  L+  ++   GV P+ +TF  +      + D    + VH  + ++G+E +I + NALI MY+K   V++   +F  M   +  SWN+++SG      C  G  +F  M      PD+ T TS++ +C  L + + G  +HA++I  G   +  V  SL  +Y   G   +AE LF+++  +D+ SWT +I+GY  +   +KAI  +  M +  V P+  T+A  LS+C+ +  L+ G  LH +AIKA     V V++++++MY+ C CI++A   F  +   + +SW  ++ G   + +  + L   R M    L+P+ +T  A L+AC+ +G +  GKE +   L    G+   + +   ++D+  R G+ N   S     K
Sbjct:   66 LHGLCANGKLEEAMKLLNSMQELRVAVDEDVFVALVRLCEWKRAQEEGSKVYSIALSSMSSLGVELGNAFLAMFVRFGNLVDAWYVFGKMSERNLFSWNVLVGGYAKQGYFDEAMCLYHRMLWVGGVKPDVYTFPCVLRTCGGIPDLARGKEVHVHVVRYGYELDIDVVNALITMYVKCGDVKSARLLFDRMPRRDIISWNAMISGYFENGMCHEGLELFFAMRGLSVDPDLMTLTSVISACELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVSWTTMISGYEYNFLPDKAIDTYRMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAIKARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEALIFLRQM-KMTLQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDFLPN--ALLDMYVRCGRMNTAWSQFNSQK 560          

HSP 5 Score: 134.035 bits (336), Expect = 2.120e-30
Identity = 89/306 (29.08%), Postives = 151/306 (49.35%), Query Frame = -2

Query: 2623 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASI 3540
            + ++  C   G     + +H  +I  GF  D+ + NSL   Y   G +  A K+  RM  +D+VSWT + SG          +D Y  M +D V P+   +   L AC+   DL  G  LH   +K  ++  V V ++L+++Y+KC  ++ A  +F  + +KNV+SW +++ G  L     + L   + M  + L+ +  TL+  L +C  +  L  G   H+ V++TG   D+F+   LLD+Y +CG        F   Q+ ++ +W+ ++    E+GQ     ELF  M    V P++ TF S+
Sbjct:  301 TSVISACELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVSWTTMISGYEYNFLPDKAIDTYRMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAIKARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEALIFLRQMKMT-LQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDFLPNALLDMYVRCGRMNTAWSQFNS-QKKDVTSWNILLTGYSERGQGSMVVELFDRMVKSRVRPDEITFISL 604          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP168_ARATH (Pentatricopeptide repeat-containing protein At2g22070 OS=Arabidopsis thaliana GN=PCMP-H41 PE=3 SV=1)

HSP 1 Score: 424.091 bits (1089), Expect = 1.682e-129
Identity = 245/701 (34.95%), Postives = 369/701 (52.64%), Query Frame = -2

Query:  922 LLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMK---------------IRSVQNGWRVFKEMXXXXXXXXXXXXXXSQ----------------NGKTCD-RGPSIFHKMLAEGF-KPDICTFTSILRSCSNLLNIKFGHQVHAHII--------------------------------KNGLKDNNLVG-TSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAF-LGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 2823
            +L  YSK G  + T + F ++ + + V+W+ MI      GQ  +A  +   M  +G+ P Q T  ++    A        + VH+ I K G    + +SN+L+ MY K               +R + + W     +         ++    Q                N +  D R   IF KML +    PD  T  S+L +C+NL  +  G Q+H+HI+                                + G KD  + G T+L+D Y K G +  A+ +F  L +RD+ +WTA+I GY Q     +AI  F  M   G  PN++TLA  LS  S++A L +G+ +H  A+K+G    V VS++++ MYA  G I  A  AF L     DTVSW  M+    QH    + LE F  ML +GL PD +T++ V SAC++ G VN+G++YFD + +V  I+PT+ H ACM+D+ GRAG   E + FI+ M +  + + W ++L  CR+H N  LG+ AAE+L  L P  +  Y  LAN+++A G+WE+  ++R  M    VKKE G SW+ V  +VH+F   DG HP+  EIY+ +K++ ++    GYVP T  +L+D+ ++ K + L HH+E+LA+AF LIS     T+RI KNL +C DCH  +K +S ++ +EI+VRD   FHHFK G CSC+DY
Sbjct:   86 VLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIVGYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISS-WNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFSACTHAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLLSACRVHKNIDLGKVAAERLLLLEPENSGAYSALANLYSACGKWEEAAKIRKSMKDGRVKKEQGFSWIEVKHKVHVFGVEDGTHPEKNEIYMTMKKIWDEIKKMGYVPDTASVLHDLEEEVKEQILRHHSEKLAIAFGLISTPDKTTLRIMKNLRVCNDCHTAIKFISKLVGREIIVRDTTRFHHFKDGFCSCRDY 785          

HSP 2 Score: 187.963 bits (476), Expect = 1.093e-47
Identity = 135/516 (26.16%), Postives = 245/516 (47.48%), Query Frame = -2

Query: 2113 WNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKC-------------------------------GQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGM-TESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLK---------------------------------VFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMX--XXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSL------IDLYAKNGFLADAELLFTQL-IERDLFSWTALIAG 3438
            WN++++AY+KRG     C+  D++P+RD VSWT +  G  N G  +  + +   M K+G+ P    L   L + + +  +  G  +H   VK+G+  +V V +SL+++YAKC                               GQM+LA   F  M ++++V+WN++++G+  +G   + L++F  M  +S L   +FTL++VL +C  L  L  G   HS ++ TG +    V   L+ +YS+CG  E   +                                 +F  +++ ++VAW+AMI   ++ G   EA  LF  M   G  PN +T A++  VA++L    + + +H    K G    + +SNALI MY K  ++ +  R F  +       +  + +++ +Q+G   +    +F  ML EG +PD  T+  +  +C++   +  G Q       + +KD + +  +L      +DL+ + G L +A+    ++ IE D+ +W +L++ 
Sbjct:   83 WNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIVGYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGH-AEEALELFETMLMEGLRPDHITYVGVFSACTHAGLVNQGRQYF-----DMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLLSA 592          

HSP 3 Score: 154.836 bits (390), Expect = 4.558e-37
Identity = 139/597 (23.28%), Postives = 259/597 (43.38%), Query Frame = -2

Query: 1735 KACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAG-------------------------------KILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCG------------LAED-------------------TLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFS-LMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWR---------------------------------VFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQL-IERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSS-IVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMMLFGYLQHRQ-GLKVLETFRMMLDKGLEPD 3225
            K+ + S   F   L+H   +K G++  V++ ++L+++Y+K G    A ++F  M  +   SWN +L+ Y+ +GD                                 K + +   M +  +  ++FTL+NVL S      +  G   HS ++K G   +  V   LL++Y+KCG            +  D                    +  F+++ E +IV W++MI   +++G    A ++FS ++R   +SP++ T AS+    ANL      + +H+ I   GF+   I+ NALI+MY +   V+   R                                 +F  +   +  +W +++ G +   +     ++F  M+  G +P+  T  ++L   S+L ++  G Q+H   +K+G   +  V  +LI +YAK G +  A   F  +  ERD  SWT++I   AQ   +E+A++ F  M  +G+ P++ T     S+C++  ++  G+    M              + +VD++   G ++EA+     M +  D V+W  +L     H+   L  +   R++L   LEP+
Sbjct:   22 KSVNKSNGRFTAQLVHCRVIKSGLMFSVYLMNNLMNVYSKTGYALHARKLFDEMPLRTAFSWNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIVGYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFSACTHAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLLSACRVHKNIDLGKVAAERLLL---LEPE 615          

HSP 4 Score: 137.502 bits (345), Expect = 1.424e-31
Identity = 100/380 (26.32%), Postives = 168/380 (44.21%), Query Frame = -2

Query: 2599 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRM-------------------------------PERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVL-PNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAER---------------------------------VFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLG 3540
            + +L   A+   + + K VH  I+K G   ++ + NSL+N YAK G    A  V DRM                                ERD+V+W ++ SG    GY    LD++  M +D +L P+   L + L AC+    L  G  +H   V  G  +   V ++L+ +Y++CG +E A R                                 +F+ +  ++VV+W A++ GY   G  G+ +NLF+ M     R + +TL+ +L   + L +L+ G   H   +K+G  +   V   L+ +Y+K G      + F  I+ E + V+W++MI  L + G  +EA ELF  M  +G+ P+  T+  +     + G
Sbjct:  150 TNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFSACTHAG 529          

HSP 5 Score: 119.398 bits (298), Expect = 7.048e-26
Identity = 96/349 (27.51%), Postives = 150/349 (42.98%), Query Frame = -2

Query: 1540 PDICTFTSILRSCSNLL-------NIKFGHQ-VHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFT-------------------------------QLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANC-------------------------------GCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETF-RMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLT----NVFGIVPTIKHCACMIDILGRAGKFNEVESFI-----KDMKV 2346
            P   + +++L  C+NLL       N +F  Q VH  +IK+GL  +  +  +L+++Y+K G+   A  LF                                QL +RD  SWT +I GY       KAI+    M ++G+ P  FTL   L+S +    +E G+ +HS  +K G  G+V VS+S+++MYA C                               G ++ A A F  M   D V+WN M+ G+ Q    L+ L+ F +M+ D  L PD  T  +VLSAC+ +  +  GK+    +     ++ GIV        +I +  R G        I     KD+K+
Sbjct:    4 PVPLSLSTLLELCTNLLQKSVNKSNGRFTAQLVHCRVIKSGLMFSVYLMNNLMNVYSKTGYALHARKLFDEMPLRTAFSWNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIVGYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAKFVFDRMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYDLRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIV-----LNALISMYSRCGGVETARRLIEQRGTKDLKI 347          

HSP 6 Score: 106.301 bits (264), Expect = 6.281e-22
Identity = 81/319 (25.39%), Postives = 139/319 (43.57%), Query Frame = -2

Query: 2725 SKILHDCASKGSLSSAKAVHGKIIKKGFD-------------------------------PDLHL--WNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVF-LFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEH-------DEFVG-----CCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMI 3540
            + +L  CA+   L   K +H  I+  GFD                                DL +  + +L++ Y K G    A  +   + +RDVV+WTA+  G    G   + ++L+ SM   G  PN + L   L   S    L  G  +HG  VK G +  V V ++L+ +YAK G +  A R F L   +++ VSW +++   A  G A + L LF+ M    LR    T   V  +CT           H+ ++  G ++       D+ +       C++DL+ + GL ++  +  +++  EP++V W +++
Sbjct:  283 ASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGYEQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFSACT-----------HAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLL 590          

HSP 7 Score: 65.0846 bits (157), Expect = 3.800e-9
Identity = 51/199 (25.63%), Postives = 93/199 (46.73%), Query Frame = -2

Query: 2914 AKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMP-ERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSS-LVDLYAKCGQMELAERVFLFMD-KKNVVSWNALLNGYALKG--DAGKILNLFQGMTESELRCSKFTLSNVLKSC 3495
             K +HG  +K G    + + N+L+  YAK G    A +  D +  ERD VSWT++   L   G+  + L+L+ +M  +G+ P+    V    AC+ +  +  G         V  ++      + +VDL+ + G ++ A+     M  + +VV+W +LL+   +    D GK+      + E E   +   L+N+  +C
Sbjct:  432 GKQIHGSAVKSGEIYSVSVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLMEGLRPDHITYVGVFSACTHAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQEAQEFIEKMPIEPDVVTWGSLLSACRVHKNIDLGKVAAERLLLLEPENSGAYSALANLYSAC 630          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP210_ARATH (Pentatricopeptide repeat-containing protein At3g03580 OS=Arabidopsis thaliana GN=PCMP-H23 PE=2 SV=1)

HSP 1 Score: 427.172 bits (1097), Expect = 1.723e-129
Identity = 254/867 (29.30%), Postives = 452/867 (52.13%), Query Frame = -2

Query:  925 ASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRM-PERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLF-HHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQD 3519
            +S  +L+  + +H  +I  G D        L++ Y+       +  V  R+ P ++V  W ++       G   + L+ Y  +++  V P+ +   + +KAC+   D   G L++ + + +G   D+FVG++LVD+Y++ G +  A +VF  M  +++VSWN+L++GY+  G   + L ++  +  S +    FT+S+VL +   L+ +  G   H   +K+G      V   L+ +Y K     D  +VF  +   + V+++ MI    +   ++E+  +F L       P+  T +S+     +L D    + ++  + K GF  E  + N LI +Y K   +     VF  M   ++ SWNS++SG            +F  M+    + D  T+  ++   + L ++KFG  +H++ IK+G+  +  V  +LID+YAK G + D+  +F+ +   D  +W  +I+   +       ++    M++  V P+  T    L  C+++A    G+ +H   ++ G   ++ + +++++MY+ CGC+E +   F  M   D V+W  M++ Y  + +G K LETF  M   G+ PD V FIA++ ACS+ G V+EG   F+ +   + I P I+H AC++D+L R+ K ++ E FI+ M +  +  IW +VL  CR  G+ +  E  + ++ ELNP    + IL +N +AA  +W+ V  +R  +    + K PG SW+ V   VH+F S D   P+   IY  L+ L       GY+P    +  ++ ++E+  +L   H+ERLA+AF L++  P   +++ KNL +C DCH+  KL+S ++ +EI+VRDAN FH FK G CSC+D
Sbjct:   15 SSSSNLNELRRIHALVISLGLDSSDFFSGKLIDKYSHFREPASSLSVFRRVSPAKNVYLWNSIIRAFSKNGLFPEALEFYGKLRESKVSPDKYTFPSVIKACAGLFDAEMGDLVYEQILDMGFESDLFVGNALVDMYSRMGLLTRARQVFDEMPVRDLVSWNSLISGYSSHGYYEEALEIYHELKNSWIVPDSFTVSSVLPAFGNLLVVKQGQGLHGFALKSGVNSVVVVNNGLVAMYLKFRRPTDARRVFDEMDVRDSVSYNTMICGYLKLEMVEESVRMF-LENLDQFKPDLLTVSSVLRACGHLRDLSLAKYIYNYMLKAGFVLESTVRNILIDVYAKCGDMITARDVFNSMECKDTVSWNSIISGYIQSGDLMEAMKLFKMMMIMEEQADHITYLMLISVSTRLADLKFGKGLHSNGIKSGICIDLSVSNALIDMYAKCGEVGDSLKIFSSMGTGDTVTWNTVISACVRFGDFATGLQVTTQMRKSEVVPDMATFLVTLPMCASLAAKRLGKEIHCCLLRFGYESELQIGNALIEMYSKCGCLENSSRVFERMSRRDVVTWTGMIYAYGMYGEGEKALETFADMEKSGIVPDSVVFIAIIYACSHSGLVDEGLACFEKMKTHYKIDPMIEHYACVVDLLSRSQKISKAEEFIQAMPIKPDASIWASVLRACRTSGDMETAERVSRRIIELNPDDPGYSILASNAYAALRKWDKVSLIRKSLKDKHITKNPGYSWIEVGKNVHVFSSGDDSAPQSEAIYKSLEILYSLMAKEGYIPDPREVSQNLEEEEEKRRLICGHSERLAIAFGLLNTEPGTPLQVMKNLRVCGDCHEVTKLISKIVGREILVRDANRFHLFKDGTCSCKD 880          

HSP 2 Score: 226.483 bits (576), Expect = 9.529e-60
Identity = 145/522 (27.78%), Postives = 272/522 (52.11%), Query Frame = -2

Query: 1978 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG 3543
            +  ++  CA          V+ +I+  GF+ DL + N+LV+ Y++ G+   A +V D MP RD+VSW +L SG  + GY  + L++Y  +K   ++P+   + + L A    L +  G  LHG  +K GV   V V + LV +Y K  +   A RVF  MD ++ VS+N ++ GY       + + +F    + + +    T+S+VL++C +L +L+     ++ ++K G   +  V   L+D+Y+KCG       VF  ++  + V+W+++I    + G + EA +LF +M       +  T+  +  V+  L D  + +G+H+   K G   ++ +SNALI MY K   V +  ++F  M + ++ +WN+++S          G  +  +M      PD+ TF   L  C++L   + G ++H  +++ G +    +G +LI++Y+K G L ++  +F ++  RD+ +WT +I  Y      EKA++ F  M++ G+ P++      + +CS+  +++ G
Sbjct:  109 FPSVIKACAGLFDAEMGDLVYEQILDMGFESDLFVGNALVDMYSRMGLLTRARQVFDEMPVRDLVSWNSLISGYSSHGYYEEALEIYHELKNSWIVPDSFTVSSVLPAFGNLLVVKQGQGLHGFALKSGVNSVVVVNNGLVAMYLKFRRPTDARRVFDEMDVRDSVSYNTMICGYLKLEMVEESVRMFLENLD-QFKPDLLTVSSVLRACGHLRDLSLAKYIYNYMLKAGFVLESTVRNILIDVYAKCGDMITARDVFNSMECKDTVSWNSIISGYIQSGDLMEAMKLFKMMMIMEEQADHITYLMLISVSTRLADLKFGKGLHSNGIKSGICIDLSVSNALIDMYAKCGEVGDSLKIFSSMGTGDTVTWNTVISACVRFGDFATGLQVTTQMRKSEVVPDMATFLVTLPMCASLAAKRLGKEIHCCLLRFGYESELQIGNALIEMYSKCGCLENSSRVFERMSRRDVVTWTGMIYAYGMYGEGEKALETFADMEKSGIVPDSVVFIAIIYACSHSGLVDEG 629          

HSP 3 Score: 98.9821 bits (245), Expect = 1.621e-19
Identity = 55/218 (25.23%), Postives = 112/218 (51.38%), Query Frame = -2

Query: 1672 ILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIE-RDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGK 2322
            I R+ S+  N+    ++HA +I  GL  ++     LID Y+     A +  +F ++   ++++ W ++I  ++++    +A++ +  ++   V+P+ +T  + + +C+ +   E G L++   +  G   D+FV +++VDMY+  G +  A   F  M   D VSWN ++ GY  H    + LE +  + +  + PD  T  +VL A   +  V +G+
Sbjct:   10 ISRALSSSSNLNELRRIHALVISLGLDSSDFFSGKLIDKYSHFREPASSLSVFRRVSPAKNVYLWNSIIRAFSKNGLFPEALEFYGKLRESKVSPDKYTFPSVIKACAGLFDAEMGDLVYEQILDMGFESDLFVGNALVDMYSRMGLLTRARQVFDEMPVRDLVSWNSLISGYSSHGYYEEALEIYHELKNSWIVPDSFTVSSVLPAFGNLLVVKQGQ 227          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A0D2SEX4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G158100 PE=4 SV=1)

HSP 1 Score: 1853.95 bits (4801), Expect = 0.000e+0
Identity = 929/944 (98.41%), Postives = 933/944 (98.83%), Query Frame = -2

Query:  919 MLIQSLVFRCLSTRKNHLVIEFPRRLLXXXXXXXXXXXXXXATEEINPSLKVPLFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDYC 3750
            MLIQSLVFRCLSTRKN LVIEFPRRLL ASFQFVSSF+AHVATEEINPSLKVPLFKEKTKLEG+KRLKLYSKILHDCASKGSLSSAKAVHGKIIK G DPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSW ALFSGLVNEGYGY VLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLD+YSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMSSWNSASWNSLLSGS NGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGD+FVSSSIVDMYANCGCIEEAEAAF GMVSTDTVSWN MLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLG CRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDG HPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDYC
Sbjct:    1 MLIQSLVFRCLSTRKNRLVIEFPRRLLFASFQFVSSFTAHVATEEINPSLKVPLFKEKTKLEGSKRLKLYSKILHDCASKGSLSSAKAVHGKIIKNGIDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWAALFSGLVNEGYGYYVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDMYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMSSWNSASWNSLLSGSHNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDMFVSSSIVDMYANCGCIEEAEAAFQGMVSTDTVSWNTMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGACRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGFHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDYC 944          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A0B0NSU1_GOSAR (Pentatricopeptide repeat-containing-like protein OS=Gossypium arboreum GN=F383_23794 PE=4 SV=1)

HSP 1 Score: 1618.98 bits (4191), Expect = 0.000e+0
Identity = 798/810 (98.52%), Postives = 801/810 (98.89%), Query Frame = -2

Query:  940 MPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGI 3369
            MPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLD+FVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIK GCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACI KHGFESEIILSNALIAMYMKIRSVQNGW+VFKEMS WNSASWNSLLSGS NGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNM MLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAF GMVSTDTVSWNMMLFGYLQHRQGLKVLETFR MLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEV+SFIKDMKVTSNPLIWETVLG CRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDV DDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGI
Sbjct:    1 MPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDIFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKIGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACILKHGFESEIILSNALIAMYMKIRSVQNGWQVFKEMSFWNSASWNSLLSGSHNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMGMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFQGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRTMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVKSFIKDMKVTSNPLIWETVLGACRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVGDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGI 810          

HSP 2 Score: 207.994 bits (528), Expect = 4.078e-52
Identity = 150/574 (26.13%), Postives = 279/574 (48.61%), Query Frame = -2

Query: 1816 LHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMML 3531
            L  C+    L     +HG+ +K G   D+ + +SLV+ YAK G    A +V   M +++VVSW AL +G   +G    +L+L+  M +  +  +   L   LK+C+  ++L +G + H   +K+G   D FVG  L+DLY+KCG  E   +VF  + + N+V+W+A+++    +G   +   LF  M    +  ++ T +++      L +  +    H+ ++K G E +  +   L+ +Y K    ++  +VFK +   N  +W++++              +F  M  +G  P+  TF SI    +NL +  +   VHA I K+G +   ++  +LI +Y K   + +   +F ++   +  SW +L++G       ++    F+ M  +G  P+  T  + L SCSN+  ++ G  +H+  IK G   +  V +S++D+YA  G + +AE  F  ++  D  SW  ++ GY Q     K ++ F  M  +G+ P+  T    LS+CS M  +  G +   S+    G    +   + ++D+    G   E ++    M V+++ + W  +L
Sbjct:   48 LKACSLSLDLFFGTLLHGEGVKVGVLLDIFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKIGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACILKHGFESEIILSNALIAMYMKIRSVQNGWQVFKEMSFWNSASWNSLLSGSHNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMGMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFQGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRTMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVKSFIKDMKVTSNPLIWETVL 621          

HSP 3 Score: 157.918 bits (398), Expect = 4.410e-36
Identity = 109/412 (26.46%), Postives = 207/412 (50.24%), Query Frame = -2

Query: 2305 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCS 3540
            S +L  C    +L+     H  +IK G + D  +   L++ Y+K G+     KV  R+ E ++V+W+A+   L  +G   +  +L+  M+  GV PN H   +     +   D  +   +H   +K G   ++ + ++L+ +Y K   ++   +VF  M   N  SWN+LL+G        +  ++F  M     +    T +++L+SC+ L+N+ +G   H+ +IK G + +  VG  L+DLY+K G   D   +F ++ E ++ +W+A+I    +    ++A + F+ M+ +GV+PN  T A+     +N+G     + +H+   K G   ++ +S++++ MY     ++     F+ M S ++ SWN +L G    +   +    F  ML +G +PD  TF ++L +CS
Sbjct:  146 SNVLKSCTYLVNLTWGLIAHSLVIKIGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACILKHGFESEIILSNALIAMYMKIRSVQNGWQVFKEMSFWNSASWNSLLSGSHNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMGMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFQGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRTMLDKGLEPDEVTFIAVLSACS 557          

HSP 4 Score: 129.798 bits (325), Expect = 3.038e-27
Identity = 76/213 (35.68%), Postives = 109/213 (51.17%), Query Frame = -2

Query: 2905 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYL 3543
            ++ IL  C++  ++     VH  IIK G   +  +  SL++ YAK G    A  +  ++ ERD+ SWTAL +G          +  +  M++ GV PN   L T L +CS    L  G LLH   +K G   DVFV SS+VD+YA CG +E AE  F  M   + VSWN +L GY       K+L  F+ M +  L   + T   VL +C+Y+
Sbjct:  347 FTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMGMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFQGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRTMLDKGLEPDEVTFIAVLSACSYM 559          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A061F6E7_THECC (Pentatricopeptide repeat superfamily protein, putative OS=Theobroma cacao GN=TCM_030800 PE=4 SV=1)

HSP 1 Score: 1569.29 bits (4062), Expect = 0.000e+0
Identity = 782/946 (82.66%), Postives = 848/946 (89.64%), Query Frame = -2

Query:  925 MLIQSLVFRCLSTRKNHLVIEFPRRLLXXXXXXXXXXXXXXATEEINP----SLKVPLFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQD 3750
            ML+ SL FRC S RK+ L I+FPRR L ASFQ VS F+  VAT EINP     LKV  FKEK KL+  KRLKLYSKILHDCASKGSL  AK VHGKI++ G DPDLHLWNSLVN YAK G FGYACKVLD+MPE DVVSWTALFSGLVNEG+G  VL LYC MKKDGV PNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCG+MELAERVF++MDKKNVVSWNALLNGYAL+GDAGK+LNLF+GMTESELRCSKFTLSNVLKSCTYL NLTWGLIAHS+VIK+GCEHDEFVGCCLLD+YSKCGLAED LKVF+RIQEPNIVAWSAMIDCLDEQGQIQEAAE+F LMR KGVSPNQHTF+SIAG A NLGD+ +CEG+HACI K+GFESE +LSNALI+MYMKIRSVQNGW+VFKEMSSW+ ASWN+LLSG  + KTCD+GP IFHKMLA GF+PDICTF SILRSCS+LLN+KFG QVHAHIIKNGL  NNLVGTSLIDLYAKN FL DAELLF+QLIERDLFSWTALIAGYAQ++  EKAIKCFN +QRQGV PN F LATCLSSCS MAMLENGQLLHSMAIKAG+S D+FVSS++VDMYANCGCIEEAE+AF GM S D VSWN MLFGYLQH QGLKVLETFR MLDKGLEPDEVTFI VLSACSYMG V EGKE+FDSLTNV+GIVPTI+H ACMIDILGRAGKFNEVESF+KD KVTS  LIWETVLG+CRMHGNDK GE AAEKLFEL+P  ASHYILLANIFAAK RW+DVRRVRALMT  GVKKEPGCSWVMVNGQ+HIFRS D  HP + EIY+KL+ELV+K ILAGYVPKT+H+L++VS  EK+EQLF HNERLALAFALISINP+KT+RIFKNL ICEDCHDFMKLVSG+I+QEIVVRD NCFHHF+SGICSCQD
Sbjct:    1 MLVPSLDFRCFSLRKHSLFIKFPRRSLLASFQLVSCFTTLVATNEINPIFYPPLKVTPFKEKVKLDRTKRLKLYSKILHDCASKGSLRLAKVVHGKIMENGLDPDLHLWNSLVNVYAKCGSFGYACKVLDKMPETDVVSWTALFSGLVNEGHGSAVLGLYCFMKKDGVRPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGEMELAERVFVYMDKKNVVSWNALLNGYALEGDAGKVLNLFEGMTESELRCSKFTLSNVLKSCTYLGNLTWGLIAHSVVIKSGCEHDEFVGCCLLDMYSKCGLAEDALKVFQRIQEPNIVAWSAMIDCLDEQGQIQEAAEMFCLMRRKGVSPNQHTFSSIAGAATNLGDQFFCEGIHACIIKYGFESENVLSNALISMYMKIRSVQNGWQVFKEMSSWDLASWNALLSGPHDDKTCDQGPIIFHKMLAAGFRPDICTFASILRSCSSLLNLKFGQQVHAHIIKNGLNGNNLVGTSLIDLYAKNRFLEDAELLFSQLIERDLFSWTALIAGYAQTNRVEKAIKCFNQIQRQGVKPNEFILATCLSSCSKMAMLENGQLLHSMAIKAGHSADLFVSSALVDMYANCGCIEEAESAFQGMASADVVSWNTMLFGYLQHGQGLKVLETFRTMLDKGLEPDEVTFIGVLSACSYMGLVEEGKEHFDSLTNVYGIVPTIEHYACMIDILGRAGKFNEVESFVKDTKVTSTALIWETVLGSCRMHGNDKFGEIAAEKLFELDPETASHYILLANIFAAKCRWDDVRRVRALMTSHGVKKEPGCSWVMVNGQLHIFRSADSSHPMNREIYMKLQELVQKVILAGYVPKTEHVLHNVSHREKMEQLFCHNERLALAFALISINPMKTVRIFKNLLICEDCHDFMKLVSGIIEQEIVVRDVNCFHHFRSGICSCQD 946          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: F6HGR7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0016g02500 PE=4 SV=1)

HSP 1 Score: 1112.83 bits (2877), Expect = 0.000e+0
Identity = 542/867 (62.51%), Postives = 672/867 (77.51%), Query Frame = -2

Query:  922 CASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3522
            CASKG L+  KA+HG++IK G +PD HLWNSLVN YAK G   YACKV   +PERDVVSWTAL +G V EGYG   ++L+C M+++GV  N     TALKACS+ LDL FG  +H E +KVG   D+FVGS+LVDLYAKCG+M LAERVFL M K+N VSWNALLNG+A  GDA K+LNLF  MT SE+  SKFTLS VLK C    NL  G I HSL I+ GCE DEF+ CCL+D+YSKCGLA D LKVF RI++P++V+WSA+I CLD++GQ +EAAE+F  MR  GV PNQ T AS+   A +LGD  Y E +HAC+ K+GFE +  + NAL+ MYMKI SVQ+G RVF+  ++ +  SWN+LLSG  + +TCD G  IF++MLAEGF P++ TF SILRSCS+L ++  G QVHA I+KN L  N+ VGT+L+D+YAKN FL DAE +F +LI+RDLF+WT ++AGYAQ    EKA+KCF  MQR+GV PN FTLA+ LS CS +A L++G+ LHSMAIKAG SGD+FV+S++VDMYA CGC+E+AE  F G+VS DTVSWN ++ GY QH QG K L+ F  MLD+G  PDEVTFI VLSACS+MG + EGK++F+SL+ ++GI PTI+H ACM+DILGRAGKF+EVESFI++MK+TSN LIWETVLG C+MHGN + GE AA KLFEL P I S+YILL+N+FAAKG W+DV  VRALM+  GVKKEPGCSWV VNGQVH+F S DG HPK  EI++KL++L +K +  GY P TDH+L++VSD EK E LF+H+ERLALAFAL+S +  KTIRIFKNL IC DCHDFMK +S +  QE+VVRD NCFHHFK+G CSCQ++
Sbjct:   43 CASKGDLNEGKAIHGQVIKSGINPDSHLWNSLVNVYAKCGSANYACKVFGEIPERDVVSWTALITGFVAEGYGSGAVNLFCEMRREGVEANEFTYATALKACSMCLDLEFGKQVHAEAIKVGDFSDLFVGSALVDLYAKCGEMVLAERVFLCMPKQNAVSWNALLNGFAQMGDAEKVLNLFCRMTGSEINFSKFTLSTVLKGCANSGNLRAGQIVHSLAIRIGCELDEFISCCLVDMYSKCGLAGDALKVFVRIEDPDVVSWSAIITCLDQKGQSREAAEVFKRMRHSGVIPNQFTLASLVSAATDLGDLYYGESIHACVCKYGFEYDNTVCNALVTMYMKIGSVQDGCRVFEATTNRDLISWNALLSGFHDNETCDTGLRIFNQMLAEGFNPNMYTFISILRSCSSLSDVDLGKQVHAQIVKNSLDGNDFVGTALVDMYAKNRFLEDAETIFNRLIKRDLFAWTVIVAGYAQDGQGEKAVKCFIQMQREGVKPNEFTLASSLSGCSRIATLDSGRQLHSMAIKAGQSGDMFVASALVDMYAKCGCVEDAEVVFDGLVSRDTVSWNTIICGYSQHGQGGKALKAFEAMLDEGTVPDEVTFIGVLSACSHMGLIEEGKKHFNSLSKIYGITPTIEHYACMVDILGRAGKFHEVESFIEEMKLTSNVLIWETVLGACKMHGNIEFGERAAMKLFELEPEIDSNYILLSNMFAAKGMWDDVTNVRALMSTRGVKKEPGCSWVEVNGQVHVFLSHDGSHPKIREIHLKLQDLHQKLMSVGYTPNTDHVLHNVSDREKQELLFYHSERLALAFALLSTSTRKTIRIFKNLRICGDCHDFMKSISEITNQELVVRDINCFHHFKNGSCSCQNF 909          

HSP 2 Score: 285.034 bits (728), Expect = 2.933e-77
Identity = 192/641 (29.95%), Postives = 331/641 (51.64%), Query Frame = -2

Query: 1627 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQL-LHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPT 3543
            Y+  L  C+    L   K VH + IK G   DL + ++LV+ YAK G    A +V   MP+++ VSW AL +G    G    VL+L+C M    +  +   L T LK C+ S +L  G ++H   +++G  LD F+   LVD+Y+KCG    A +VF+ ++  +VVSW+A++     KG + +   +F+ M  S +  ++FTL++++ + T L +L +G   H+ V K G E+D  V   L+ +Y K G  +D  +VF+     ++++W+A++    +         +F+ M  +G +PN +TF SI    ++L D    + VHA I K+  +    +  AL+ MY K R +++   +F  +   +  +W  +++G       ++    F +M  EG KP+  T  S L  CS +  +  G Q+H+  IK G   +  V ++L+D+YAK G + DAE++F  L+ RD  SW  +I GY+Q     KA+K F  M  +G  P+  T    LS+CS+M ++E G+   +S++   G +  +   + +VD+    G   E E+    M ++++ + W  +L G  +    ++  E   M L + LEP+  +   +LS       +   K  +D +TNV  ++ T
Sbjct:  137 YATALKACSMCLDLEFGKQVHAEAIKVGDFSDLFVGSALVDLYAKCGEMVLAERVFLCMPKQNAVSWNALLNGFAQMGDAEKVLNLFCRMTGSEINFSKFTLSTVLKGCANSGNLRAGQIVHSLAIRIGCELDEFISCCLVDMYSKCGLAGDALKVFVRIEDPDVVSWSAIITCLDQKGQSREAAEVFKRMRHSGVIPNQFTLASLVSAATDLGDLYYGESIHACVCKYGFEYDNTVCNALVTMYMKIGSVQDGCRVFEATTNRDLISWNALLSGFHDNETCDTGLRIFNQMLAEGFNPNMYTFISILRSCSSLSDVDLGKQVHAQIVKNSLDGNDFVGTALVDMYAKNRFLEDAETIFNRLIKRDLFAWTVIVAGYAQDGQGEKAVKCFIQMQREGVKPNEFTLASSLSGCSRIATLDSGRQLHSMAIKAGQSGDMFVASALVDMYAKCGCVEDAEVVFDGLVSRDTVSWNTIICGYSQHGQGGKALKAFEAMLDEGTVPDEVTFIGVLSACSHMGLIEEGKKHFNSLSKIYGITPTIEHYACMVDILGRAGKFHEVESFIEEMKLTSNVLIWETVL-GACKMHGNIEFGERAAMKLFE-LEPEIDSNYILLSN------MFAAKGMWDDVTNVRALMST 769          

HSP 3 Score: 196.052 bits (497), Expect = 5.969e-48
Identity = 129/417 (30.94%), Postives = 218/417 (52.28%), Query Frame = -2

Query: 1669 SCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKE 2919
            +C    +L  G   H  VIK+G   D  +   L+++Y+KCG A    KVF  I E ++V+W+A+I     +G    A  LF  MR +GV  N+ T+A+     +   D  + + VHA   K G  S++ + +AL+ +Y K   +    RVF  M   N+ SWN+LL+G       ++  ++F +M          T +++L+ C+N  N++ G  VH+  I+ G + +  +   L+D+Y+K G   DA  +F ++ + D+ SW+A+I    Q   S +A + F  M+  GV PN FTLA+ +S+ +++  L  G+ +H+   K G   D  V +++V MY   G +++    F    + D +SWN +L G+  +      L  F  ML +G  P+  TFI++L +CS +  V+ GK+
Sbjct:   42 TCASKGDLNEGKAIHGQVIKSGINPDSHLWNSLVNVYAKCGSANYACKVFGEIPERDVVSWTALITGFVAEGYGSGAVNLFCEMRREGVEANEFTYATALKACSMCLDLEFGKQVHAEAIKVGDFSDLFVGSALVDLYAKCGEMVLAERVFLCMPKQNAVSWNALLNGFAQMGDAEKVLNLFCRMTGSEINFSKFTLSTVLKGCANSGNLRAGQIVHSLAIRIGCELDEFISCCLVDMYSKCGLAGDALKVFVRIEDPDVVSWSAIITCLDQKGQSREAAEVFKRMRHSGVIPNQFTLASLVSAATDLGDLYYGESIHACVCKYGFEYDNTVCNALVTMYMKIGSVQDGCRVFEATTNRDLISWNALLSGFHDNETCDTGLRIFNQMLAEGFNPNMYTFISILRSCSSLSDVDLGKQ 458          

HSP 4 Score: 166.392 bits (420), Expect = 1.590e-38
Identity = 124/391 (31.71%), Postives = 195/391 (49.87%), Query Frame = -2

Query: 1258 KTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDIL---GRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGES--AAEKLFEL-NPGIASHYILLANIFAAKGRWEDV---RRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCH 2403
            +TCD+GP I  ++L EGF+P++        +C++  ++  G  +H  +IK+G+  ++ +  SL+++YAK G    A  +F ++ ERD+ SWTALI G+        A+  F  M+R+GV  N FT AT L +CS    LE G+ +H+ AIK G+  D+FV S++VD+YA CG +  AE  FL M   + VSWN +L G+ Q     KVL  F  M    +   + T   VL  C+  G +  G +   SL    G         C++D+    G AG   +V   I+D  V S    W  ++ TC     D+ G+S  AAE    + + G+  +   LA++ +A     D+     + A +   G + +      +V   + I    DGC 
Sbjct:   20 ETCDQGPRILIQLLVEGFEPNM--------TCASKGDLNEGKAIHGQVIKSGINPDSHLWNSLVNVYAKCGSANYACKVFGEIPERDVVSWTALITGFVAEGYGSGAVNLFCEMRREGVEANEFTYATALKACSMCLDLEFGKQVHAEAIKVGDFSDLFVGSALVDLYAKCGEMVLAERVFLCMPKQNAVSWNALLNGFAQMGDAEKVLNLFCRMTGSEINFSKFTLSTVLKGCANSGNLRAG-QIVHSLAIRIGCELDEFISCCLVDMYSKCGLAGDALKVFVRIEDPDVVS----WSAII-TCL----DQKGQSREAAEVFKRMRHSGVIPNQFTLASLVSAATDLGDLYYGESIHACVCKYGFEYDNTVCNALVTMYMKIGSVQDGCR 392          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: U5FHM8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0018s06910g PE=4 SV=1)

HSP 1 Score: 1067.37 bits (2759), Expect = 0.000e+0
Identity = 517/870 (59.43%), Postives = 660/870 (75.86%), Query Frame = -2

Query:  925 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQD 3534
            +L +CASKG +   KA+HG +I  G + D HLW SL+N YAK     +A KVL  MP+RDVVSWTAL SG VNEG G + + LYC M+K+ V  N   L TALKACS+ L+L FG  +H E +K G+LLD+FVG++LVDLYA+CG+MELAER+F  M  KN VSWNALLNGYA  GD  K+L LF  M E E + SKFTLS VLK C    +L  G + H+L +++GCE DEF+GC L+D+YSKCG   D LKVF +I+ P++VAWSAMI  LD+QG  QEAAELF LMR KG  PNQ T +S+   A N+GD  Y + +H CI K+GFES+ ++SN LI MYMK R V++G +VF+ M++ +  SWN+LLSG  + +TC RGP IF++ML EGFKP++ TF S+LRSCS+LL+ +FG QVHAHIIKN   D++ VGT+L+D+YAK   L DA + F +L+ RD+FSWT +I+GYAQ+D +EKA+K F  MQR+G+ PN +TLA+CLS CS+MA LENG+ LH++A+KAG+ GD+FV S++VD+Y  CGC+E AEA F G++S D VSWN ++ GY QH QG K LE FRMML +G+ PDE TFI VLSACS+MG V EGK+ FDS++ ++GI P+I+H ACM+DILGRAGKFNEV+ FI++M +T   LIWETVLG C++HGN   GE AA+KLFE+ P + S YILL+NIFA+KGRW+DVR +RALMT  G+KKEPGCSWV V+GQVH+F S DG HPK  EIY KL +L +  +  GYVPKT+ +L++VS+ EK+E L++H+ERLAL+FAL+S N VK IRIFKNL ICEDCHDFMKL+S +  QEIVVRD   FHHFK G CSCQD
Sbjct:    1 MLRECASKGDVKEGKAIHGNLITSGVELDSHLWVSLINFYAKCRSRFFARKVLAEMPQRDVVSWTALISGFVNEGCGSESVSLYCEMRKENVRANEFALATALKACSMCLNLEFGKQVHVEAIKAGLLLDLFVGTALVDLYARCGEMELAERLFFGMPDKNGVSWNALLNGYAQLGDGKKVLKLFCKMKECETKFSKFTLSTVLKGCANTGSLREGKVLHALALRSGCEIDEFLGCSLVDMYSKCGTVYDALKVFTKIRNPDVVAWSAMITGLDQQGHGQEAAELFHLMRRKGARPNQFTLSSLVSTATNMGDLRYGQSIHGCICKYGFESDNLVSNPLIMMYMKSRCVEDGNKVFEAMTNPDLVSWNALLSGFYDSQTCGRGPRIFYQMLLEGFKPNMFTFISVLRSCSSLLDPEFGKQVHAHIIKNSSDDDDFVGTALVDMYAKARCLEDAGVAFDRLVNRDIFSWTVIISGYAQTDQAEKAVKYFRQMQREGIKPNEYTLASCLSGCSHMATLENGRQLHAVAVKAGHFGDIFVGSALVDLYGKCGCMEHAEAIFKGLISRDIVSWNTIISGYSQHGQGEKALEAFRMMLSEGIMPDEATFIGVLSACSFMGLVEEGKKRFDSMSKIYGINPSIEHYACMVDILGRAGKFNEVKIFIEEMNLTPYSLIWETVLGACKLHGNVDFGEKAAKKLFEMEPMMDSSYILLSNIFASKGRWDDVRNIRALMTSRGIKKEPGCSWVEVDGQVHVFLSQDGSHPKIREIYAKLDKLGQSLMSIGYVPKTEVVLHNVSNKEKMEHLYYHSERLALSFALLSTNAVKPIRIFKNLRICEDCHDFMKLISDITNQEIVVRDIRRFHHFKRGTCSCQD 870          

HSP 2 Score: 204.912 bits (520), Expect = 6.243e-51
Identity = 128/412 (31.07%), Postives = 217/412 (52.67%), Query Frame = -2

Query: 2305 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCS 3540
            S +L  CA+ GSL   K +H   ++ G + D  L  SLV+ Y+K G    A KV  ++   DVV+W+A+ +GL  +G+G +  +L+  M++ G  PN   L + +   +   DL +G  +HG   K G   D  V + L+ +Y K   +E   +VF  M   ++VSWNALL+G+      G+   +F  M     + + FT  +VL+SC+ L++  +G   H+ +IK   + D+FVG  L+D+Y+K    ED    F R+   +I +W+ +I    +  Q ++A + F  M+ +G+ PN++T AS     +++        +HA   K G   +I + +AL+ +Y K   +++   +FK + S +  SWN+++SG       ++    F  ML+EG  PD  TF  +L +CS
Sbjct:  201 STVLKGCANTGSLREGKVLHALALRSGCEIDEFLGCSLVDMYSKCGTVYDALKVFTKIRNPDVVAWSAMITGLDQQGHGQEAAELFHLMRRKGARPNQFTLSSLVSTATNMGDLRYGQSIHGCICKYGFESDNLVSNPLIMMYMKSRCVEDGNKVFEAMTNPDLVSWNALLSGFYDSQTCGRGPRIFYQMLLEGFKPNMFTFISVLRSCSSLLDPEFGKQVHAHIIKNSSDDDDFVGTALVDMYAKARCLEDAGVAFDRLVNRDIFSWTVIISGYAQTDQAEKAVKYFRQMQREGIKPNEYTLASCLSGCSHMATLENGRQLHAVAVKAGHFGDIFVGSALVDLYGKCGCMEHAEAIFKGLISRDIVSWNTIISGYSQHGQGEKALEAFRMMLSEGIMPDEATFIGVLSACS 612          

HSP 3 Score: 137.887 bits (346), Expect = 1.117e-29
Identity = 91/285 (31.93%), Postives = 140/285 (49.12%), Query Frame = -2

Query: 2734 LEGAK-RLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWG-----LIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWS 3570
            LEG K  +  +  +L  C+S       K VH  IIK   D D  +  +LV+ YAK      A    DR+  RD+ SWT + SG          +  +  M+++G+ PN + L + L  CS    L  G  LH   VK G   D+FVGS+LVDLY KCG ME AE +F  +  +++VSWN +++GY+  G   K L  F+ M    +   + T   VL +C+++  +  G      ++    I    EH      C++D+  + G   + +K+F  I+E N+  +S
Sbjct:  392 LEGFKPNMFTFISVLRSCSSLLDPEFGKQVHAHIIKNSSDDDDFVGTALVDMYAKARCLEDAGVAFDRLVNRDIFSWTVIISGYAQTDQAEKAVKYFRQMQREGIKPNEYTLASCLSGCSHMATLENGRQLHAVAVKAGHFGDIFVGSALVDLYGKCGCMEHAEAIFKGLISRDIVSWNTIISGYSQHGQGEKALEAFRMMLSEGIMPDEATFIGVLSACSFMGLVEEGKKRFDSMSKIYGINPSIEHY----ACMVDILGRAGKFNE-VKIF--IEEMNLTPYS 669          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: M5VT93_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020478mg PE=4 SV=1)

HSP 1 Score: 1060.83 bits (2742), Expect = 0.000e+0
Identity = 525/871 (60.28%), Postives = 656/871 (75.32%), Query Frame = -2

Query:  922 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3534
            +L  C  +GSL+  KA+HG++IK G DPDLHLW SLVN YAK G  GYA KVLD MPE+DVVSWT L  G V  G+G D + L+C MKKDG   N   L T LKACSL  DL FG  LH E VK+G   DVFVGS+LV LYAKCG+MELA+ V   M ++NVVSWNALLNGYA +GD  ++L LF  MTESE+R SKFTLS VLK C    NL  G   HSL IK+GC+ DEF+GC L+D+YSKCG+A D +KVF+RI+ P++VAWSA+I CLD+QGQ QE AELF  M   G+SPNQ + +SI   A +L D  + E VHA  +K+G ES+I +SNALI MYMKI  V +G +VF+ M+  +  SWNSLLSG  N + CD GP IF +ML EGFKP++ +F S+LRSCS+LL++  G QVHAHI+K  L DN+ VGT+LID+YAK  FL DA + F +L  RDLF WT +I GYAQ+D +EKA+ CF+ MQ++GV PN F LA CLS+CS +AMLENG+ LHSMAIK+G+ GD+FVSS++VDMYA CGCI +AE  F G+ S DTVSWN+M+ GY Q+ +G K +E F  ML++G  PDEVTFI +LSACS++G V EGK++FDSL+ VF I PTI+H ACM+DIL RAGKFNE ESFI+ MK+T  P+IWETVLG C+M+GN + GE+AA+KLFEL P + S YILL+NIFA KGRW+DV +VR LM+  GVKK+PGCSWV V+GQV+ F S DG HP+  +I++KL+EL EK    GY+P+T+ +L+++++ EK E L +H+ERLALAF+LIS NP KTIRIFKNL IC DCH+ MKL+S V  +EIVVRD   FHHFKSG CSC D+
Sbjct:    1 MLRTCVLQGSLNEGKAIHGQVIKNGIDPDLHLWVSLVNVYAKCGDCGYARKVLDEMPEQDVVSWTTLIQGFVVNGFGVDAVKLFCEMKKDGTRANEFALATGLKACSLCFDLGFGKQLHAEAVKLGFFSDVFVGSALVGLYAKCGEMELADTVLFCMPEQNVVSWNALLNGYAQEGDGKQVLKLFCRMTESEMRLSKFTLSTVLKGCANSENLRGGQFLHSLAIKSGCKIDEFLGCSLVDMYSKCGMAIDAVKVFRRIKNPDVVAWSAIITCLDQQGQCQEVAELFREMISTGISPNQFSLSSIISAATDLKDLHFGESVHAFAWKYGCESDISVSNALITMYMKIGRVLDGAQVFEAMTDRDLISWNSLLSGMHNHEICDLGPRIFRQMLVEGFKPNMYSFISVLRSCSSLLDVGLGKQVHAHIVKTSLDDNDFVGTALIDMYAKIRFLEDAVIAFNKLSNRDLFIWTVIITGYAQTDQAEKAVACFSQMQQEGVKPNEFALAGCLSACSRIAMLENGRQLHSMAIKSGHLGDLFVSSALVDMYAKCGCIGDAEDIFGGLDSCDTVSWNIMICGYSQYGRGEKAIEAFSTMLNEGTIPDEVTFIGILSACSHLGLVEEGKKHFDSLSKVFRITPTIEHYACMVDILVRAGKFNEAESFIETMKLTLYPIIWETVLGACKMYGNVEFGETAAKKLFELKPEMDSTYILLSNIFAVKGRWDDVSKVRKLMSSQGVKKKPGCSWVEVDGQVNTFVSQDGSHPRIRDIHLKLEELGEKLNSVGYIPETEDVLHNITEREKNEHLQYHSERLALAFSLISTNPPKTIRIFKNLRICGDCHEVMKLISDVTNREIVVRDIKRFHHFKSGTCSCNDF 871          

HSP 2 Score: 181.415 bits (459), Expect = 1.914e-43
Identity = 120/414 (28.99%), Postives = 211/414 (50.97%), Query Frame = -2

Query: 2299 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNL 3540
            S +L  CA+  +L   + +H   IK G   D  L  SLV+ Y+K G+   A KV  R+   DVV+W+A+ + L  +G   +V +L+  M   G+ PN   L + + A +   DL FG  +H    K G   D+ V ++L+ +Y K G++    +VF  M  ++++SWN+LL+G            +F+ M     + + ++  +VL+SC+ L+++  G   H+ ++KT  + ++FVG  L+D+Y+K    ED +  F ++   ++  W+ +I    +  Q ++A   FS M+ +GV PN+   A      + +        +H+   K G   ++ +S+AL+ MY K   + +   +F  + S ++ SWN ++ G       ++    F  ML EG  PD  TF  IL +CS+L
Sbjct:  201 STVLKGCANSENLRGGQFLHSLAIKSGCKIDEFLGCSLVDMYSKCGMAIDAVKVFRRIKNPDVVAWSAIITCLDQQGQCQEVAELFREMISTGISPNQFSLSSIISAATDLKDLHFGESVHAFAWKYGCESDISVSNALITMYMKIGRVLDGAQVFEAMTDRDLISWNSLLSGMHNHEICDLGPRIFRQMLVEGFKPNMYSFISVLRSCSSLLDVGLGKQVHAHIVKTSLDDNDFVGTALIDMYAKIRFLEDAVIAFNKLSNRDLFIWTVIITGYAQTDQAEKAVACFSQMQQEGVKPNEFALAGCLSACSRIAMLENGRQLHSMAIKSGHLGDLFVSSALVDMYAKCGCIGDAEDIFGGLDSCDTVSWNIMICGYSQYGRGEKAIEAFSTMLNEGTIPDEVTFIGILSACSHL 614          

HSP 3 Score: 117.472 bits (293), Expect = 2.432e-23
Identity = 89/312 (28.53%), Postives = 138/312 (44.23%), Query Frame = -2

Query: 2665 PLFKEKTKLEGAKRLKLYS--KILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMR 3594
            P    +  +EG K   +YS   +L  C+S   +   K VH  I+K   D +  +  +L++ YAK      A    +++  RD+  WT + +G          +  +  M+++GV PN   L   L ACS    L  G  LH   +K G L D+FV S+LVD+YAKCG +  AE +F  +D  + VSWN ++ GY+  G   K +  F  M        + T   +L +C++L     GL      ++ G +H                   D+L    RI  P I  ++ M+D L   G+  EA      M+
Sbjct:  384 PRIFRQMLVEGFKP-NMYSFISVLRSCSSLLDVGLGKQVHAHIVKTSLDDNDFVGTALIDMYAKIRFLEDAVIAFNKLSNRDLFIWTVIITGYAQTDQAEKAVACFSQMQQEGVKPNEFALAGCLSACSRIAMLENGRQLHSMAIKSGHLGDLFVSSALVDMYAKCGCIGDAEDIFGGLDSCDTVSWNIMICGYSQYGRGEKAIEAFSTMLNEGTIPDEVTFIGILSACSHL-----GL------VEEGKKH------------------FDSLSKVFRIT-PTIEHYACMVDILVRAGKFNEAESFIETMK 664          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A0A0K4Y9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G388380 PE=4 SV=1)

HSP 1 Score: 984.171 bits (2543), Expect = 0.000e+0
Identity = 484/879 (55.06%), Postives = 631/879 (71.79%), Query Frame = -2

Query:  922 KRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3558
            K+LK YS +L +CASK SL  AKA+HG I+K   +PD HLW SLVN YAK     YA  VL +MP+RDVVSWTAL  GLV EG+  D + L+  M+ +G++PN   L T LKACSL + L  G  +H +  K+G+LLD+FVGS+LVDLYAKCG++ELA ++F+ M ++N V+WN LLNGYA +GD   +L LF  M E +++C++FTL+ VLK C    NL  G + HSL+IK G E +EF+GC L+D+YSKCGLA D + VFK I++P+IV WSA+I CLD+QGQ +E+ +LF LMR     PNQ+T  S+   A N G+  Y + +HAC++K+GFE+++ +SNAL+ MYMK   V +G ++++ M   +  SWN+ LSG  +    DR  +IF+ ML EGF P++ TF SIL SCS L ++ +G QVHAHIIKN L DNN V T+LID+YAK  +L DA++ F +L  RDLF+WT +I  YAQ++  EKA+  F  MQ++GV PN FTLA CLS CS++A LE GQ LHSM  K+G+  D+FV S++VDMYA CGC+EEAEA F  ++  DT++WN ++ GY Q+ QG K L  FRMMLD+G+ PD VTF  +LSACS+ G V EGKE+F+S+   FGI PT+ HCACM+DILGR GKF+E+E FI+ M+++ N LIWETVLG  +MH N  LGE AA KLFEL P   S YILL+NIFA +GRW+DV+RVR+LM+  GVKKEPGCSWV  NGQVH F S D  HP+  EI++KL EL  +     YVPKT+++L++V + EK E L  H+ERLAL FALIS +  K IRIFKNL IC DCHD MK +S +  QEIVVRD   FHHFK+G CSC D+
Sbjct:  215 KKLKYYSSMLRECASKRSLGVAKAIHGLIVKDVINPDSHLWVSLVNVYAKCRYSAYARLVLAKMPDRDVVSWTALIQGLVAEGFANDSIYLFQEMQNEGIMPNEFTLATGLKACSLCMALDLGKQMHAQAFKLGLLLDLFVGSALVDLYAKCGEIELASKMFIGMPEQNDVTWNVLLNGYAQRGDVTGVLKLFCSMMELDVKCNEFTLTTVLKGCANSKNLKQGQVIHSLIIKCGYEGNEFIGCGLVDMYSKCGLAIDAIGVFKTIKKPDIVVWSALITCLDQQGQSEESIKLFHLMRLGDTLPNQYTICSLLSAATNTGNLQYGQSIHACVWKYGFETDVAVSNALVTMYMKNGCVHDGTKLYESMVDRDLISWNAYLSGLHDCGMYDRPLTIFYHMLEEGFIPNMYTFISILGSCSCLFDVHYGRQVHAHIIKNQLDDNNFVCTALIDMYAKCMYLEDADVAFNRLSVRDLFTWTVIITNYAQTNQGEKALNYFRQMQQEGVKPNEFTLAGCLSGCSSLASLEGGQQLHSMVFKSGHVSDMFVGSALVDMYAKCGCMEEAEALFEALIRRDTIAWNTIICGYAQNGQGNKALTAFRMMLDEGISPDGVTFTGILSACSHQGLVEEGKEHFNSMYRDFGISPTVDHCACMVDILGRVGKFDELEDFIQKMQLSQNALIWETVLGASKMHNNLVLGEKAANKLFELQPEEESSYILLSNIFATEGRWDDVKRVRSLMSSKGVKKEPGCSWVEANGQVHTFVSHDYSHPQIQEIHLKLDELDRELASIQYVPKTEYVLHNVGETEKKENLRFHSERLALGFALISTSSEKKIRIFKNLRICRDCHDVMKHISSITNQEIVVRDVRRFHHFKNGACSCNDF 1093          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: M1BCU9_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG402016414 PE=4 SV=1)

HSP 1 Score: 966.066 bits (2496), Expect = 0.000e+0
Identity = 473/882 (53.63%), Postives = 630/882 (71.43%), Query Frame = -2

Query:  922 EGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3567
            E  + +K YS++L D A+K  L   KA+HG++I+ G +PD HLW SL+N Y+K G   +A  V D +P RDVVSWTAL +G + +GYG   + L+C M+ + + PN   L T LK CS+ LDL FG  LH   VK  V  DV+VGS+LVDLYAKC ++E A +VF  M ++N VSWN LLNGY   G   + L LF  M++SE+R S +TLS +LK C   VNL  G + HS+++K G E D+F  C LLD+Y+KCGL +D LKVF R +  +IVAW+AMI  LD+QGQ +EA +LF LM   G+ PNQ T AS+   AA+  D   C+ +HAC++K GF+SE  +SNALIAMYMK  SV +G+R+F  +S+ +  SWNSLLSG  + +T   GP IF ++L EG +P+I T  S LRSC++LL+   G QVHAH++K  L  N  VGT+L+D+YAK G L DAEL+F +L E+D+F+WT +I+GYAQSD  EKA +CFN MQR+ + PN FTLA+CL  CS +A L+NGQ LHS+ +K+G   D++V+S+++DMYA  GCI++AE+ F  M S+DTV WN +++ Y QH    K L+TFR ML +G+ PD +TFIAVLSACS++G V EG+E+FDS+ N FGI P+I+H ACM+DILGRAGKF E+E FI+ M++  + LIWETVLG C+ HGN +L E AA  LFE++P   S YILL+NI+A+KGRW DV  VRALM+  GVKKEPGCSW+ ++ QVH+F S D  HP+  +I+ KL+EL  +    GY+P T+++L++VSD EK++ L HH+ERLALAFAL+S +   TIRIFKNL IC DCH+FMKL S V  +EIV+RD N FHHF  G CSC+DY
Sbjct:  108 EKKRGIKWYSEMLKDYAAKLCLKEGKALHGEMIRSGVEPDSHLWVSLINFYSKCGDLVFAENVFDLIPSRDVVSWTALIAGFIAQGYGSKGICLFCDMRGEDIRPNEFTLATVLKGCSMCLDLEFGKQLHAVVVKGAVFSDVYVGSALVDLYAKCCELESAVKVFFSMPEQNSVSWNVLLNGYVQAGQGEEALKLFMKMSDSEMRFSNYTLSTILKGCANSVNLKAGQVIHSMLVKIGSEIDDFTSCSLLDMYNKCGLQDDALKVFLRTKNHDIVAWTAMISGLDQQGQKREAIQLFCLMMHSGLRPNQFTLASVVSAAADSVDLRCCKSIHACVYKFGFDSEECVSNALIAMYMKFGSVLDGYRIFSSLSNRDIISWNSLLSGFHDNETSYEGPKIFRQLLVEGLRPNIYTLISNLRSCASLLDASLGKQVHAHVVKADLGGNIYVGTALVDMYAKCGQLDDAELIFYRLSEKDVFTWTVVISGYAQSDQGEKAFRCFNQMQREAIKPNEFTLASCLKGCSRIASLDNGQQLHSVVMKSGQFSDMYVASALIDMYAKSGCIKDAESLFQSMESSDTVLWNTIIYAYSQHGLDEKALKTFRTMLSEGILPDGITFIAVLSACSHLGLVKEGQEHFDSIKNGFGITPSIEHYACMVDILGRAGKFTEMEHFIEGMELAPDALIWETVLGVCKAHGNVELAEKAANTLFEIDPKAESSYILLSNIYASKGRWADVSTVRALMSRQGVKKEPGCSWIEIDNQVHVFLSQDASHPRLKDIHKKLEELASRITATGYIPNTNYVLHNVSDKEKIDNLSHHSERLALAFALVSSSRNSTIRIFKNLCICGDCHEFMKLASIVTNREIVIRDINRFHHFSHGTCSCKDY 989          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: K4ASY3_SOLLC (Uncharacterized protein OS=Solanum lycopersicum GN=Solyc01g007850.2 PE=4 SV=1)

HSP 1 Score: 954.896 bits (2467), Expect = 0.000e+0
Identity = 469/882 (53.17%), Postives = 624/882 (70.75%), Query Frame = -2

Query:  922 EGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3567
            E  + +K YS++  D A K  L   KA+HG++I+ G +PD +LW SL+N Y+K G   +A  V D +P RDVVSWTAL +G + +GYG   + L+C MK + + PN   L T LK CS+ LDL FG  LH   VK     DV+VGS+LVDLYAKC ++E A +VF  M ++N VSWN LLNGY   G   + L LF  M++SE+R S +TLS +LK C   VNL  G + HS+++K G E D+F  C LLD+Y+KCGL +D LKVF R +  +IVAW+AMI  LD+QGQ +EA  LF LM   G+ PNQ T AS+   AA+  D   C+ +HAC++K GF+SE  + NALIAMYMK  SV +G+R+F  +S+ +  SWNSLLSG  + +T   GP IF ++L EG KP+I T  S LRSC++LL+   G QVHAH++K  L  N  VGT+L+D+YAK G L DAEL+F +L E+D+F+WT +I+GYAQSD  EKA +CFN MQR+ + PN FTLA+CL  CS +A L+NG+ LHS+ +K+G   D++V+S+++DMYA  GCI++AE+ F  M S+DTV WN +++ Y QH    + L+TFR ML +G+ PD +TFIAVLSACS++G V EG+ +FDS+ N FGI P+I+H ACM+DILGRAGKF E+E FI+ M +  + LIWETVLG C+ HGN +L E AA  LFE++P   S YILL+NI+A+KGRW DV  VRALM+  GVKKEPGCSW+ ++ QVH+F S D  HP+  +I+ KL+EL  +   AGY+P T+++L++VSD EK++ L HH+ERLALAFAL+S +   TIRIFKNL IC DCH+FMKL S V  +EIV+RD N FHHF  G CSC+DY
Sbjct:  136 EKKRGIKWYSEMFKDYAGKLCLKEGKALHGEMIRSGVEPDSYLWVSLINFYSKCGDLVFAENVFDLIPSRDVVSWTALIAGFIAQGYGSKGICLFCDMKGEDIRPNEFTLATVLKGCSMCLDLEFGKQLHAVVVKGAAFSDVYVGSALVDLYAKCCELESAVKVFFSMPEQNSVSWNVLLNGYVQAGQGEEALKLFLKMSDSEMRFSNYTLSTILKGCANSVNLKAGQVIHSMLVKIGSEIDDFTSCSLLDMYNKCGLQDDALKVFLRTKNHDIVAWTAMISGLDQQGQKREAIHLFCLMMHSGLRPNQFTLASVVSAAADSVDIRCCKSIHACVYKFGFDSEECVCNALIAMYMKFGSVLDGYRIFSSLSNRDIISWNSLLSGFHDNETSYEGPKIFRQLLVEGLKPNIYTLISNLRSCASLLDASLGKQVHAHVVKADLGGNIYVGTALVDMYAKCGQLDDAELIFYRLSEKDVFTWTVVISGYAQSDQGEKAFRCFNQMQREAIKPNEFTLASCLKGCSRIASLDNGRQLHSVVMKSGQFSDMYVASALIDMYAKSGCIKDAESLFQSMESSDTVLWNTIIYAYSQHGLDEEALKTFRTMLSEGIPPDGITFIAVLSACSHLGLVKEGRRHFDSIKNGFGITPSIEHYACMVDILGRAGKFTEMEHFIEGMALAPDALIWETVLGVCKAHGNVELAEKAANTLFEIDPKAESSYILLSNIYASKGRWADVSTVRALMSRQGVKKEPGCSWIEIDNQVHVFLSQDASHPRLKDIHKKLEELTSRITAAGYIPNTNYVLHNVSDKEKIDNLSHHSERLALAFALMSSSRNSTIRIFKNLCICGDCHEFMKLASIVTNREIVIRDINRFHHFSHGTCSCKDY 1017          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A068V2Q8_COFCA (Coffea canephora DH200=94 genomic scaffold, scaffold_80 OS=Coffea canephora GN=GSCOC_T00040403001 PE=4 SV=1)

HSP 1 Score: 952.97 bits (2462), Expect = 0.000e+0
Identity = 474/884 (53.62%), Postives = 629/884 (71.15%), Query Frame = -2

Query:  922 LEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCS-EKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3570
            L+  K L+ YS+    CA    L+  K  H  +IK G DPD HL+ SL+N YAK G   +A +VLD MPE+DVVSWTAL SG V EG G + ++L+C M+++G+ PN   L T L+ACS+   L FG  LH E VK     DV+VGS+LVDLYAKCG+ME A++VF  M ++N VSWN LLNGYA  GD  K+L LF  MTES++R S +TLS VLK C    +L  G + HS+ IK G   D+F+ C L+D+YSKC LA D L+VFK I++P+IV WS MI  LD+QGQ  +A ELF LM   G+ PNQ + +++   A +LGD  +C+ +HACI+K  FES++ +SNALI MYMK+  V +G +VF  MS  +  SWN+LLSG  +G++ D+GP IF KML EG  P+  TF S LRSC++ LN  FG QVHA+++KN L  +  VG +LID+Y+K   L D EL+F +L ERD+F+WT LIAGYAQ+D   EKA+  FN M R+GV  N FTLA+CLS+C+ +A L NGQ LHS AIK+G+  DV+V+S++VDMY  CGCI++AE  F  + + DTV WN M+ G+ +H Q  K L++F  ML+K ++P+ V+FI VLSACS+MG V EGK++F  ++ ++GI P++ H ACM+DILGRAG+F+E+ESFI+ MK+  N LIWETVLG C++HGN ++GE AA+KLFE++P   S YI L+NI+AAKGRW DV R+RALM+  GVKKEPGCSWV V+ + H+F S D  HP+  +IY KL++L ++    GY P T ++L++V D  K E LFHH+ERLALAFAL+S      IRIFKNL IC DCH+FMK VS +  +EIV+RD+N FHHF +GICSC+DY
Sbjct:  124 LDRGKVLRWYSETFRYCAVNLCLNEGKVFHANLIKIGIDPDSHLFVSLINFYAKCGALSFARRVLDEMPEKDVVSWTALISGFVAEGLGQEGVELFCEMRREGIRPNEFTLATVLRACSMVSGLEFGKQLHAEVVKGEAFTDVYVGSALVDLYAKCGEMEYADKVFFIMPEQNAVSWNVLLNGYAQLGDGHKLLILFCKMTESDMRFSNYTLSTVLKGCASSRSLWAGQVVHSMAIKIGSAFDDFISCGLVDMYSKCELANDALQVFKMIRDPDIVTWSTMISGLDQQGQKLQAVELFRLMMQSGLRPNQFSLSTVVSTATDLGDPHFCKSIHACIWKFHFESDLSVSNALITMYMKLGLVYDGLKVFSAMSQKDVVSWNALLSGYHDGESSDQGPMIFKKMLTEGLMPNQYTFISTLRSCTSQLNASFGKQVHAYLLKNNLCTDVHVGIALIDMYSKCRCLDDVELIFNRLSERDIFTWTVLIAGYAQTDNQGEKALGFFNRMHREGVKANEFTLASCLSACAGIASLINGQQLHSWAIKSGHFCDVYVASALVDMYGKCGCIDDAEMIFKSIETVDTVLWNTMICGHSKHGQNEKALQSFGAMLNKDVQPNGVSFIGVLSACSHMGLVEEGKKHFHLMSELYGIAPSVDHYACMVDILGRAGRFSELESFIQHMKIAPNTLIWETVLGACKIHGNVEMGEKAAQKLFEIDPDEDSSYIWLSNIYAAKGRWNDVSRIRALMSSRGVKKEPGCSWVEVDAKTHVFLSQDASHPRLTDIYQKLEDLHQRLQSIGYTPNTHYVLHNVPDAVKKENLFHHSERLALAFALVSNANGGRIRIFKNLRICGDCHEFMKGVSDITNKEIVIRDSNRFHHFHNGICSCKDY 1007