g.barbadense_cottongen_reftransV1_0091917, g.barbadense_cottongen_reftransV1_0091917 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PPR48_ARATH (Pentatricopeptide repeat-containing protein At1g18485 OS=Arabidopsis thaliana GN=PCMP-H8 PE=2 SV=2)

HSP 1 Score: 453.751 bits (1166), Expect = 1.650e-138
Identity = 274/844 (32.46%), Postives = 440/844 (52.13%), Query Frame = -2

Query:  922 LVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSM-KKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESE----LRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKG--VSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFI-KDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3429
            ++  YA  G    +  V D +  +++  W A+ S         +VL+ +  M     +LP+       +KAC+   D+  G  +HG  VK G++ DVFVG++LV  Y   G +  A ++F  M ++N+VSWN+++  ++  G + +   L   M E            TL  VL  C     +  G   H   +K   + +  +   L+D+YSKCG   +   +FK     N+V+W+ M+     +G      ++   M   G  V  ++ T  +   V  +       + +H    K  F    +++NA +A Y K  S+    RVF  + S    SWN+L+ G               +M   G  PD  T  S+L +CS L +++ G +VH  II+N L+ +  V  S++ LY   G L   + LF  + ++ L SW  +I GY Q+   ++A+  F  M   G+     ++     +CS +  L  G+  H+ A+K     D F++ S++DMYA  G I ++   F G+    T SWN M+ GY  H    + ++ F  M   G  PD++TF+ VL+AC++ G ++EG  Y D + + FG+ P +KH AC+ID+LGRAG+ ++    + ++M   ++  IW+++L +CR+H N ++GE  A KLFEL P    +Y+LL+N++A  G+WEDVR+VR  M    ++K+ GCSW+ +N +V  F   +       EI      L  K    GY P T  + +D+S++EK+EQL  H+E+LAL + LI  +   TIR++KNL IC DCH+  KL+S V+++EIVVRD   FHHFK+G+CSC DY
Sbjct:  126 IITMYAMCGSPDDSRFVFDALRSKNLFQWNAVISSYSRNELYDEVLETFIEMISTTDLLPDHFTYPCVIKACAGMSDVGIGLAVHGLVVKTGLVEDVFVGNALVSFYGTHGFVTDALQLFDIMPERNLVSWNSMIRVFSDNGFSEESFLLLGEMMEENGDGAFMPDVATLVTVLPVCAREREIGLGKGVHGWAVKLRLDKELVLNNALMDMYSKCGCITNAQMIFKMNNNKNVVSWNTMVGGFSAEGDTHGTFDVLRQMLAGGEDVKADEVTILNAVPVCFHESFLPSLKELHCYSLKQEFVYNELVANAFVASYAKCGSLSYAQRVFHGIRSKTVNSWNALIGGHAQSNDPRLSLDAHLQMKISGLLPDSFTVCSLLSACSKLKSLRLGKEVHGFIIRNWLERDLFVYLSVLSLYIHCGELCTVQALFDAMEDKSLVSWNTVITGYLQNGFPDRALGVFRQMVLYGIQLCGISMMPVFGACSLLPSLRLGREAHAYALKHLLEDDAFIACSLIDMYAKNGSITQSSKVFNGLKEKSTASWNAMIMGYGIHGLAKEAIKLFEEMQRTGHNPDDLTFLGVLTACNHSGLIHEGLRYLDQMKSSFGLKPNLKHYACVIDMLGRAGQLDKALRVVAEEMSEEADVGIWKSLLSSCRIHQNLEMGEKVAAKLFELEPEKPENYVLLSNLYAGLGKWEDVRKVRQRMNEMSLRKDAGCSWIELNRKVFSFVVGERFLDGFEEIKSLWSILEMKISKMGYRPDTMSVQHDLSEEEKIEQLRGHSEKLALTYGLIKTSEGTTIRVYKNLRICVDCHNAAKLISKVMEREIVVRDNKRFHHFKNGVCSCGDY 969          

HSP 2 Score: 226.483 bits (576), Expect = 1.909e-59
Identity = 146/564 (25.89%), Postives = 282/564 (50.00%), Query Frame = -2

Query: 1873 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKK---DGV-LPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTE--SELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEA 3543
            Y  ++  CA    +    AVHG ++K G   D+ + N+LV+ Y   G    A ++ D MPER++VSW ++     + G+  +   L   M +   DG  +P+   LVT L  C+   ++  G  +HG  VK+ +  ++ + ++L+D+Y+KCG +  A+ +F   + KNVVSWN ++ G++ +GD     ++ + M     +++  + T+ N +  C +   L      H   +K    ++E V    +  Y+KCG      +VF  I+   + +W+A+I    +    + + +    M+  G+ P+  T  S+    + L      + VH  I ++  E ++ +  +++++Y+    +     +F  M   +  SWN++++G       DR   +F +M+  G +    +   +  +CS L +++ G + HA+ +K+ L+D+  +  SLID+YAKNG +  +  +F  L E+   SW A+I GY     +++AIK F  MQR G  P++ T    L++C++  ++  G + L  M    G   ++   + ++DM    G +++A
Sbjct:  190 YPCVIKACAGMSDVGIGLAVHGLVVKTGLVEDVFVGNALVSFYGTHGFVTDALQLFDIMPERNLVSWNSMIRVFSDNGFSEESFLLLGEMMEENGDGAFMPDVATLVTVLPVCAREREIGLGKGVHGWAVKLRLDKELVLNNALMDMYSKCGCITNAQMIFKMNNNKNVVSWNTMVGGFSAEGDTHGTFDVLRQMLAGGEDVKADEVTILNAVPVCFHESFLPSLKELHCYSLKQEFVYNELVANAFVASYAKCGSLSYAQRVFHGIRSKTVNSWNALIGGHAQSNDPRLSLDAHLQMKISGLLPDSFTVCSLLSACSKLKSLRLGKEVHGFIIRNWLERDLFVYLSVLSLYIHCGELCTVQALFDAMEDKSLVSWNTVITGYLQNGFPDRALGVFRQMVLYGIQLCGISMMPVFGACSLLPSLRLGREAHAYALKHLLEDDAFIACSLIDMYAKNGSITQSSKVFNGLKEKSTASWNAMIMGYGIHGLAKEAIKLFEEMQRTGHNPDDLTFLGVLTACNHSGLIHEGLRYLDQMKSSFGLKPNLKHYACVIDMLGRAGQLDKA 753          

HSP 3 Score: 138.658 bits (348), Expect = 9.373e-32
Identity = 102/419 (24.34%), Postives = 189/419 (45.11%), Query Frame = -2

Query: 1669 NLTWGLIAHSLVI-KTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELF-SLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAE----GFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQG--VTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKE 2901
            ++  G   H LV   T   +D+ +   ++ +Y+ CG  +D+  VF  ++  N+  W+A+I          E  E F  ++    + P+  T+  +    A + D      VH  + K G   ++ + NAL++ Y     V +  ++F  M   N  SWNS++    +    +    +  +M+ E     F PD+ T  ++L  C+    I  G  VH   +K  L    ++  +L+D+Y+K G + +A+++F     +++ SW  ++ G++    +         M   G  V  +  T+   +  C + + L + + LH  ++K     +  V+++ V  YA CG +  A+  F G+ S    SWN ++ G+ Q       L+    M   GL PD  T  ++LSACS +  +  GKE
Sbjct:   99 DIEMGRKIHQLVSGSTRLRNDDVLCTRIITMYAMCGSPDDSRFVFDALRSKNLFQWNAVISSYSRNELYDEVLETFIEMISTTDLLPDHFTYPCVIKACAGMSDVGIGLAVHGLVVKTGLVEDVFVGNALVSFYGTHGFVTDALQLFDIMPERNLVSWNSMIRVFSDNGFSEESFLLLGEMMEENGDGAFMPDVATLVTVLPVCAREREIGLGKGVHGWAVKLRLDKELVLNNALMDMYSKCGCITNAQMIFKMNNNKNVVSWNTMVGGFSAEGDTHGTFDVLRQMLAGGEDVKADEVTILNAVPVCFHESFLPSLKELHCYSLKQEFVYNELVANAFVASYAKCGSLSYAQRVFHGIRSKTVNSWNALIGGHAQSNDPRLSLDAHLQMKISGLLPDSFTVCSLLSACSKLKSLRLGKE 517          

HSP 4 Score: 107.842 bits (268), Expect = 2.769e-22
Identity = 80/300 (26.67%), Postives = 142/300 (47.33%), Query Frame = -2

Query: 2671 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGL-----IAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVF--KRIQEPNIVAWSAMIDCLD-----EQGQIQEAAELFSL 3534
            +L  C+   SL   K VHG II+   + DL ++ S+++ Y   G       + D M ++ +VSW  + +G +  G+    L ++  M   G+   G  ++    ACSL   L  G   H   +K  +  D F+  SL+D+YAK G +  + +VF  + +K+  SWNA++ GY + G A + + LF+ M  +       T   VL +C +   +  GL     +  S  +K   +H      C++D+  + G  +  L+V   +  +E ++  W +++         E G+ + AA+LF L
Sbjct:  502 LLSACSKLKSLRLGKEVHGFIIRNWLERDLFVYLSVLSLYIHCGELCTVQALFDAMEDKSLVSWNTVITGYLQNGFPDRALGVFRQMVLYGIQLCGISMMPVFGACSLLPSLRLGREAHAYALKHLLEDDAFIACSLIDMYAKNGSITQSSKVFNGLKEKSTASWNAMIMGYGIHGLAKEAIKLFEEMQRTGHNPDDLTFLGVLTACNHSGLIHEGLRYLDQMKSSFGLKPNLKH----YACVIDMLGRAGQLDKALRVVAEEMSEEADVGIWKSLLSSCRIHQNLEMGE-KVAAKLFEL 796          

HSP 5 Score: 90.5077 bits (223), Expect = 6.997e-17
Identity = 67/286 (23.43%), Postives = 130/286 (45.45%), Query Frame = -2

Query: 1483 ILRSCSNLLNIKFGHQVHAHII-KNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHM-QRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGY----LQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGN 2322
            +L++     +I+ G ++H  +     L++++++ T +I +YA  G   D+  +F  L  ++LF W A+I+ Y++++  ++ ++ F  M     + P++FT    + +C+ M+ +  G  +H + +K G   DVFV +++V  Y   G + +A   F  M   + VSWN M+  +          L + E      D    PD  T + VL  C+    +  GK        +      + + A M D+  + G     +   K M    N + W T++G     G+
Sbjct:   90 LLQASGKRKDIEMGRKIHQLVSGSTRLRNDDVLCTRIITMYAMCGSPDDSRFVFDALRSKNLFQWNAVISSYSRNELYDEVLETFIEMISTTDLLPDHFTYPCVIKACAGMSDVGIGLAVHGLVVKTGLVEDVFVGNALVSFYGTHGFVTDALQLFDIMPERNLVSWNSMIRVFSDNGFSEESFLLLGEMMEENGDGAFMPDVATLVTVLPVCAREREIGLGKGVHGWAVKLRLDKELVLNNALM-DMYSKCGCITNAQMIFK-MNNNKNVVSWNTMVGGFSAEGD 373          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP390_ARATH (Pentatricopeptide repeat-containing protein At5g16860 OS=Arabidopsis thaliana GN=PCMP-H92 PE=2 SV=1)

HSP 1 Score: 447.973 bits (1151), Expect = 1.146e-137
Identity = 271/806 (33.62%), Postives = 430/806 (53.35%), Query Frame = -2

Query:  922 LLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKN--VVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCK-GVSPNQHTFASIAGVAANLG-----DRLYCEGVHACIFKHGF----------------ESEIILSN----------ALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPS-----IFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIK-------NGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLI--ERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQG--VTPNNFTLATCLSSCSNMAMLENGQLLHSMAIK-AGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3186
            L+H + +  G+L  + + S L+  Y   G +  A  +       +  V  WN+L+  Y   G A K L LF  M         +T   V K+C  + ++  G  AH+L + TG   + FVG  L+ +YS+C    D  KVF  +   ++V+W+++I+   + G+ + A E+FS M  + G  P+  T  ++    A+LG      +L+C  V + + ++ F                E+  + SN          A++A Y +I   ++  R+F++M            S + +G    RG       +  +ML+ G KP+  T  S+L  C+++  +  G ++H + IK       NG  D N+V   LID+YAK   +  A  +F  L   ERD+ +WT +I GY+Q   + KA++  + M  +     PN FT++  L +C+++A L  G+ +H+ A++   N+  +FVS+ ++DMYA CG I +A   F  M++ + V+W  ++ GY  H  G + L  F  M   G + D VT + VL ACS+ G +++G EYF+ +  VFG+ P  +H AC++D+LGRAG+ N     I++M +   P++W   L  CR+HG  +LGE AAEK+ EL       Y LL+N++A  GRW+DV R+R+LM H GVKK PGCSWV        F   D  HP   EIY  L + +++    GYVP+T   L+DV D+EK + LF H+E+LALA+ +++      IRI KNL +C DCH     +S +I  +I++RD++ FHHFK+G CSC+ Y
Sbjct:   46 LIHQKLLSFGIL-TLNLTSHLISTYISVGCLSHAVSLLRRFPPSDAGVYHWNSLIRSYGDNGCANKCLYLFGLMHSLSWTPDNYTFPFVFKACGEISSVRCGESAHALSLVTGFISNVFVGNALVAMYSRCRSLSDARKVFDEMSVWDVVSWNSIIESYAKLGKPKVALEMFSRMTNEFGCRPDNITLVNVLPPCASLGTHSLGKQLHCFAVTSEMIQNMFVGNCLVDMYAKCGMMDEANTVFSNMSVKDVVSWNAMVAGYSQIGRFEDAVRLFEKMQEEKIKMDVVTWSAAISGYA-QRGLGYEALGVCRQMLSSGIKPNEVTLISVLSGCASVGALMHGKEIHCYAIKYPIDLRKNGHGDENMVINQLIDMYAKCKKVDTARAMFDSLSPKERDVVTWTVMIGGYSQHGDANKALELLSEMFEEDCQTRPNAFTISCALVACASLAALRIGKQIHAYALRNQQNAVPLFVSNCLIDMYAKCGSISDARLVFDNMMAKNEVTWTSLMTGYGMHGYGEEALGIFDEMRRIGFKLDGVTLLVVLYACSHSGMIDQGMEYFNRMKTVFGVSPGPEHYACLVDLLGRAGRLNAALRLIEEMPMEPPPVVWVAFLSCCRIHGKVELGEYAAEKITELASNHDGSYTLLSNLYANAGRWKDVTRIRSLMRHKGVKKRPGCSWVEGIKGTTTFFVGDKTHPHAKEIYQVLLDHMQRIKDIGYVPETGFALHDVDDEEKDDLLFEHSEKLALAYGILTTPQGAAIRITKNLRVCGDCHTAFTYMSRIIDHDIILRDSSRFHHFKNGSCSCKGY 849          

HSP 2 Score: 217.624 bits (553), Expect = 6.109e-57
Identity = 176/700 (25.14%), Postives = 302/700 (43.14%), Query Frame = -2

Query: 1645 KTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERD--VVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMT-ESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMR------------------------------CK-----GVSPNQHTFASIAGVAANLGDRLYCEGVHACIFK---------HGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGF------KPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNL-VGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNV 3579
            K  L      ++    +H C +   +S  K +H K++  G    L+L + L++ Y   G   +A  +L R P  D  V  W +L     + G     L L+  M      P+ +      KAC     +  G   H   +  G + +VFVG++LV +Y++C  +  A +VF  M   +VVSWN+++  YA  G     L +F  MT E   R    TL NVL  C  L   + G   H   + +    + FVG CL+D+Y+KCG+ ++   VF  +   ++V+W+AM+    + G+ ++A  LF  M+                              C+     G+ PN+ T  S+    A++G  ++ + +H    K         HG E+ +I  N LI MY K + V     +F  +S          +      +  D   ++  ++L+E F      +P+  T +  L +C++L  ++ G Q+HA+ ++N      L V   LID+YAK G ++DA L+F  ++ ++  +WT+L+ GY      E+A+  F+ M+R G   +  TL   L +CS+  M++ G +  + M    G S      + +VD+    G +  A                                   R++ +  +EP  V ++A LS C   G V  G+   + +T +
Sbjct:   19 KVSLFSTSAPEITPPFIHKCKT---ISQVKLIHQKLLSFGI-LTLNLTSHLISTYISVGCLSHAVSLLRRFPPSDAGVYHWNSLIRSYGDNGCANKCLYLFGLMHSLSWTPDNYTFPFVFKACGEISSVRCGESAHALSLVTGFISNVFVGNALVAMYSRCRSLSDARKVFDEMSVWDVVSWNSIIESYAKLGKPKVALEMFSRMTNEFGCRPDNITLVNVLPPCASLGTHSLGKQLHCFAVTSEMIQNMFVGNCLVDMYAKCGMMDEANTVFSNMSVKDVVSWNAMVAGYSQIGRFEDAVRLFEKMQEEKIKMDVVTWSAAISGYAQRGLGYEALGVCRQMLSSGIKPNEVTLISVLSGCASVGALMHGKEIHCYAIKYPIDLRKNGHGDENMVI--NQLIDMYAKCKKVDTARAMFDSLSPKERDVVTWTVMIGGYSQHGDANKAL--ELLSEMFEEDCQTRPNAFTISCALVACASLAALRIGKQIHAYALRNQQNAVPLFVSNCLIDMYAKCGSISDARLVFDNMMAKNEVTWTSLMTGYGMHGYGEEALGIFDEMRRIGFKLDGVTLLVVLYACSHSGMIDQGMEYFNRMKTVFGVSPGPEHYACLVDLLGRAGRLNAA----------------------------------LRLIEEMPMEPPPVVWVAFLSCCRIHGKVELGEYAAEKITEL 676          

HSP 3 Score: 105.916 bits (263), Expect = 1.094e-21
Identity = 75/296 (25.34%), Postives = 136/296 (45.95%), Query Frame = -2

Query: 1444 LRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERD--LFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETF-RMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGE-SAAEKLFE 2319
            +  C  +  +K  HQ    ++  G+   NL  + LI  Y   G L+ A  L  +    D  ++ W +LI  Y  + C+ K +  F  M     TP+N+T      +C  ++ +  G+  H++++  G   +VFV +++V MY+ C  + +A   F  M   D VSWN ++  Y +  +    LE F RM  + G  PD +T + VL  C+ +G  + GK+          ++  +    C++D+  + G  +E  +   +M V  + + W  ++      G  ++G    A +LFE
Sbjct:   35 IHKCKTISQVKLIHQ---KLLSFGILTLNLT-SHLISTYISVGCLSHAVSLLRRFPPSDAGVYHWNSLIRSYGDNGCANKCLYLFGLMHSLSWTPDNYTFPFVFKACGEISSVRCGESAHALSLVTGFISNVFVGNALVAMYSRCRSLSDARKVFDEMSVWDVVSWNSIIESYAKLGKPKVALEMFSRMTNEFGCRPDNITLVNVLPPCASLGTHSLGKQ-LHCFAVTSEMIQNMFVGNCLVDMYAKCGMMDEANTVFSNMSV-KDVVSWNAMVA-----GYSQIGRFEDAVRLFE 319          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP252_ARATH (Pentatricopeptide repeat-containing protein At3g24000, mitochondrial OS=Arabidopsis thaliana GN=PCMP-H87 PE=3 SV=1)

HSP 1 Score: 435.647 bits (1119), Expect = 1.229e-135
Identity = 220/553 (39.78%), Postives = 331/553 (59.86%), Query Frame = -2

Query:  922 VHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 2577
            VHA I +  F  +I++ N L+ MY K  S++   +VF++M   +  +    +   SQ+ + CD     F++ML  G+ P+  T +S++++ +       GHQ+H   +K G   N  VG++L+DLY + G + DA+L+F  L  R+  SW ALIAG+A+   +EKA++ F  M R G  P++F+ A+   +CS+   LE G+ +H+  IK+G     F  ++++DMYA  G I +A   F  +   D VSWN +L  Y QH  G + +  F  M   G+ P+E++F++VL+ACS+ G ++EG  Y++ L    GIVP   H   ++D+LGRAG  N    FI++M +     IW+ +L  CRMH N +LG  AAE +FEL+P     +++L NI+A+ GRW D  RVR  M   GVKKEP CSWV +   +H+F + D  HP+  EI  K +E++ K    GYVP T H++  V   E+   L +H+E++ALAFAL++  P  TI I KN+ +C DCH  +KL S V+ +EI+VRD N FHHFK G CSC+DY
Sbjct:   82 VHAHILQSIFRHDIVMGNTLLNMYAKCGSLEEARKVFEKMPQRDFVTWTTLISGYSQHDRPCD-ALLFFNQMLRFGYSPNEFTLSSVIKAAAAERRGCCGHQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALESRNDVSWNALIAGHARRSGTEKALELFQGMLRDGFRPSHFSYASLFGACSSTGFLEQGKWVHAYMIKSGEKLVAFAGNTLLDMYAKSGSIHDARKIFDRLAKRDVVSWNSLLTAYAQHGFGKEAVWWFEEMRRVGIRPNEISFLSVLTACSHSGLLDEGWHYYE-LMKKDGIVPEAWHYVTVVDLLGRAGDLNRALRFIEEMPIEPTAAIWKALLNACRMHKNTELGAYAAEHVFELDPDDPGPHVILYNIYASGGRWNDAARVRKKMKESGVKKEPACSWVEIENAIHMFVANDERHPQREEIARKWEEVLAKIKELGYVPDTSHVIVHVDQQEREVNLQYHSEKIALAFALLNTPPGSTIHIKKNIRVCGDCHTAIKLASKVVGREIIVRDTNRFHHFKDGNCSCKDY 632          

HSP 2 Score: 195.667 bits (496), Expect = 9.969e-51
Identity = 130/386 (33.68%), Postives = 195/386 (50.52%), Query Frame = -2

Query: 2572 LVIEFPRRLLXXXXXXXXXXXXXXATEEI--NPSLKVP-----LFKEKTKLEGA---KRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVH 3699
            + + FP RLLS         S      E   + SLK P     L      LEG+      + Y+ +L  C     L   + VH  I++  F  D+ + N+L+N YAK G    A KV ++MP+RD V+WT L SG        D L  +  M + G  PN   L + +KA +       G  LHG  VK G   +V VGS+L+DLY + G M+ A+ VF  ++ +N VSWNAL+ G+A +    K L LFQGM     R S F+ +++  +C+    L  G   H+ +IK+G +   F G  LLD+Y+K G   D  K+F R+ + ++V+W++++    + G  +EA   F  MR  G+ PN+ +F S+    ++ G  L  EG H
Sbjct:    1 MALRFPPRLLSQLKLSRRLNSLPAPVSEDSEDESLKFPSNDLLLRTSSNDLEGSYIPADRRFYNTLLKKCTVFKLLIQGRIVHAHILQSIFRHDIVMGNTLLNMYAKCGSLEEARKVFEKMPQRDFVTWTTLISGYSQHDRPCDALLFFNQMLRFGYSPNEFTLSSVIKAAAAERRGCCGHQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALESRNDVSWNALIAGHARRSGTEKALELFQGMLRDGFRPSHFSYASLFGACSSTGFLEQGKWVHAYMIKSGEKLVAFAGNTLLDMYAKSGSIHDARKIFDRLAKRDVVSWNSLLTAYAQHGFGKEAVWWFEEMRRVGIRPNEISFLSVLTACSHSG--LLDEGWH 384          

HSP 3 Score: 149.443 bits (376), Expect = 8.547e-36
Identity = 83/286 (29.02%), Postives = 151/286 (52.80%), Query Frame = -2

Query: 1486 FTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCA----CMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHG 2331
            + ++L+ C+    +  G  VHAHI+++  + + ++G +L+++YAK G L +A  +F ++ +RD  +WT LI+GY+Q D    A+  FN M R G +PN FTL++ + + +       G  LH   +K G   +V V S+++D+Y   G +++A+  F  + S + VSWN ++ G+ +     K LE F+ ML  G  P   ++ ++  ACS  GF+ +GK       + + I    K  A     ++D+  ++G  ++    I D     + + W ++L     HG
Sbjct:   63 YNTLLKKCTVFKLLIQGRIVHAHILQSIFRHDIVMGNTLLNMYAKCGSLEEARKVFEKMPQRDFVTWTTLISGYSQHDRPCDALLFFNQMLRFGYSPNEFTLSSVIKAAAAERRGCCGHQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALESRNDVSWNALIAGHARRSGTEKALELFQGMLRDGFRPSHFSYASLFGACSSTGFLEQGK-----WVHAYMIKSGEKLVAFAGNTLLDMYAKSGSIHDARK-IFDRLAKRDVVSWNSLLTAYAQHG 342          

HSP 4 Score: 145.206 bits (365), Expect = 1.856e-34
Identity = 106/373 (28.42%), Postives = 184/373 (49.33%), Query Frame = -2

Query: 2119 TALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQL-IERDLFSWTALI 3234
            T LK C++   L  G ++H   ++     D+ +G++L+++YAKCG +E A +VF  M +++ V+W  L++GY+        L  F  M       ++FTLS+V+K+         G   H   +K G + +  VG  LLDLY++ GL +D   VF  ++  N V+W+A+I     +   ++A ELF  M   G  P+  ++AS+ G  ++ G     + VHA + K G +      N L+ MY K  S+ +  ++F  ++  +  SWNSLL+              F +M   G +P+  +F S+L +CS+   +  G   +  + K+G+        +++DL  + G L  A     ++ IE     W AL+
Sbjct:   65 TLLKKCTVFKLLIQGRIVHAHILQSIFRHDIVMGNTLLNMYAKCGSLEEARKVFEKMPQRDFVTWTTLISGYSQHDRPCDALLFFNQMLRFGYSPNEFTLSSVIKAAAAERRGCCGHQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALESRNDVSWNALIAGHARRSGTEKALELFQGMLRDGFRPSHFSYASLFGACSSTGFLEQGKWVHAYMIKSGEKLVAFAGNTLLDMYAKSGSIHDARKIFDRLAKRDVVSWNSLLTAYAQHGFGKEAVWWFEEMRRVGIRPNEISFLSVLTACSHSGLLDEGWHYYELMKKDGIVPEAWHYVTVVDLLGRAGDLNRALRFIEEMPIEPTAAIWKALL 437          

HSP 5 Score: 125.946 bits (315), Expect = 2.737e-28
Identity = 75/257 (29.18%), Postives = 132/257 (51.36%), Query Frame = -2

Query: 2719 VHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDC 3486
            +HG  +K GFD ++H+ ++L++ Y + G+   A  V D +  R+ VSW AL +G          L+L+  M +DG  P+     +   ACS +  L  G  +H   +K G  L  F G++L+D+YAK G +  A ++F  + K++VVSWN+LL  YA  G   + +  F+ M    +R ++ +  +VL +C++   L  G   + L+ K G   + +    ++DL  + G     L+  + +  EP    W A+++ 
Sbjct:  183 LHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALESRNDVSWNALIAGHARRSGTEKALELFQGMLRDGFRPSHFSYASLFGACSSTGFLEQGKWVHAYMIKSGEKLVAFAGNTLLDMYAKSGSIHDARKIFDRLAKRDVVSWNSLLTAYAQHGFGKEAVWWFEEMRRVGIRPNEISFLSVLTACSHSGLLDEGWHYYELMKKDGIVPEAWHYVTVVDLLGRAGDLNRALRFIEEMPIEPTAAIWKALLNA 439          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PPR32_ARATH (Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H40 PE=2 SV=1)

HSP 1 Score: 437.573 bits (1124), Expect = 3.491e-134
Identity = 259/750 (34.53%), Postives = 405/750 (54.00%), Query Frame = -2

Query:  922 KVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEG--VHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3165
            K G+  + F  + LV L+ + G ++ A RVF  +D K  V ++ +L G+A   D  K L  F  M   ++    +  + +LK C     L  G   H L++K+G   D F    L ++Y+KC    +  KVF R+ E ++V+W+ ++    + G  + A E+   M  + + P+  T  S+    + L  RL   G  +H    + GF+S + +S AL+ MY K  S++   ++F  M   N  SWNS++      +       IF KML EG KP   +    L +C++L +++ G  +H   ++ GL  N  V  SLI +Y K   +  A  +F +L  R L SW A+I G+AQ+     A+  F+ M+ + V P+ FT  + +++ + +++  + + +H + +++    +VFV++++VDMYA CG I  A   F  M      +WN M+ GY  H  G   LE F  M    ++P+ VTF++V+SACS+ G V  G + F  +   + I  ++ H   M+D+LGRAG+ NE   FI  M V     ++  +LG C++H N    E AAE+LFELNP    +++LLANI+ A   WE V +VR  M   G++K PGCS V +  +VH F S    HP   +IY  L++L+     AGYVP T+ +L  V +D K + L  H+E+LA++F L++     TI + KNL +C DCH+  K +S V  +EIVVRD   FHHFK+G CSC DY
Sbjct:   62 KNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSAL--RLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKENYSIELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEKAAERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGCSMVEIKNEVHSFFSGSTAHPDSKKIYAFLEKLICHIKEAGYVPDTNLVLG-VENDVKEQLLSTHSEKLAISFGLLNTTAGTTIHVRKNLRVCADCHNATKYISLVTGREIVVRDMQRFHHFKNGACSCGDY 808          

HSP 2 Score: 227.254 bits (578), Expect = 2.713e-60
Identity = 161/603 (26.70%), Postives = 287/603 (47.60%), Query Frame = -2

Query: 1708 SLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVS--SSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLS 3507
            SL   + +   + K G   +      LV+ + + G    A +V + +  +  V +  +  G          L  +  M+ D V P  +     LK C    +L  G  +HG  VK G  LD+F  + L ++YAKC Q+  A +VF  M ++++VSWN ++ GY+  G A   L + + M E  L+ S  T+ +VL + + L  ++ G   H   +++G +    +   L+D+Y+KCG  E   ++F  + E N+V+W++MID   +    +EA  +F  M  +GV P   +        A+LGD      +H    + G +  + + N+LI+MY K + V     +F ++ S    S    +L  +QNG+  D   + F +M +   KPD  T+ S++ + + L        +H  ++++ L  N  V T+L+D+YAK G +  A L+F  + ER + +W A+I GY      + A++ F  MQ+  + PN  T  + +S+CS+  ++E G     M +K   S ++ +    ++VD+    G + EA    + M     V+    + G  Q  + +   E     L + L PD+  +  +L+
Sbjct:   49 SLKELRQILPLVFKNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPID-ALNYFSQMRSRTVKPDTFTYVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYM-MKENYSIELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEKAAERLFE-LNPDDGGYHVLLA 648          

HSP 3 Score: 201.06 bits (510), Expect = 9.402e-52
Identity = 123/434 (28.34%), Postives = 218/434 (50.23%), Query Frame = -2

Query: 2242 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLK 3543
            ++ +L  C  +  L   K +HG ++K GF  DL     L N YAK      A KV DRMPERD+VSW  + +G    G     L++  SM ++ + P+   +V+ L A S    +  G  +HG  ++ G    V + ++LVD+YAKCG +E A ++F  M ++NVVSWN++++ Y    +  + + +FQ M +  ++ +  ++   L +C  L +L  G   H L ++ G + +  V   L+ +Y KC   +    +F ++Q   +V+W+AMI    + G+  +A   FS MR + V P+  T+ S+    A L    + + +H  + +   +  + ++ AL+ MY K  ++     +F  MS  +  +WN+++ G            +F +M     KP+  TF S++ +CS           H+ +++ GLK
Sbjct:  138 FTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACS-----------HSGLVEAGLK 560          

HSP 4 Score: 115.931 bits (289), Expect = 7.563e-25
Identity = 75/290 (25.86%), Postives = 135/290 (46.55%), Query Frame = -2

Query: 1477 ILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEY--------FDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDK 2322
            +L  CS+L  ++   Q+   + KNGL   +   T L+ L+ + G + +A  +F  +  +    +  ++ G+A+    +KA++ F  M+   V P  +     L  C + A L  G+ +H + +K+G S D+F  + + +MYA C  + EA   F  M   D VSWN ++ GY Q+      LE  + M ++ L+P  +T ++VL A S +  ++ GKE         FDSL N+            ++D+  + G           M +  N + W +++     + N K
Sbjct:   43 LLERCSSLKELR---QILPLVFKNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNI---------STALVDMYAKCGSLETARQLFDGM-LERNVVSWNSMIDAYVQNENPK 319          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP377_ARATH (Putative pentatricopeptide repeat-containing protein At5g13230, mitochondrial OS=Arabidopsis thaliana GN=PCMP-H89 PE=3 SV=1)

HSP 1 Score: 437.573 bits (1124), Expect = 4.814e-134
Identity = 253/794 (31.86%), Postives = 419/794 (52.77%), Query Frame = -2

Query:  922 LDLYCSMKKDGVLP--NGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKT-IRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3294
            LDL  S   D ++P  + H     L+ C    D      +H + +K G  LD+F  + L++ Y K G  + A  +F  M ++N VS+  L  GYA +   G    L+  +       +    ++ LK    L         HS ++K G + + FVG  L++ YS CG  +    VF+ I   +IV W+ ++ C  E G  +++ +L S MR  G  PN +TF +    +  LG   + +GVH  I K  +  +  +   L+ +Y ++  + + ++VF EM   +   W+ +++       C+    +F +M      P+  T +SIL  C+       G Q+H  ++K G   +  V  +LID+YAK   +  A  LF +L  ++  SW  +I GY       KA   F    R  V+    T ++ L +C+++A ++ G  +H +AIK  N+  V VS+S++DMYA CG I+ A++ F  M + D  SWN ++ GY  H  G + L    +M D+  +P+ +TF+ VLS CS  G +++G+E F+S+    GI P ++H  CM+ +LGR+G+ ++    I+ +    + +IW  +L       N++    +AE++ ++NP   + Y+L++N++A   +W +V  +R  M   GVKKEPG SW+   G VH F      HP    I   L+ L  K+  AGYVP  + +L D+ D+EK ++L+ H+ERLALA+ L+ +   +  I I KNL IC DCH  MK++S ++++++V+RD N FHHF +G+CSC D+
Sbjct:   35 LDLESS---DSIIPGLDSHAYGAMLRRCIQKNDPISAKAIHCDILKKGSCLDLFATNILLNAYVKAGFDKDALNLFDEMPERNNVSFVTLAQGYACQDPIG----LYSRLHREGHELNPHVFTSFLKLFVSLDKAEICPWLHSPIVKLGYDSNAFVGAALINAYSVCGSVDSARTVFEGILCKDIVVWAGIVSCYVENGYFEDSLKLLSCMRMAGFMPNNYTFDTALKASIGLGAFDFAKGVHGQILKTCYVLDPRVGVGLLQLYTQLGDMSDAFKVFNEMPKNDVVPWSFMIARFCQNGFCNEAVDLFIRMREAFVVPNEFTLSSILNGCAIGKCSGLGEQLHGLVVKVGFDLDIYVSNALIDVYAKCEKMDTAVKLFAELSSKNEVSWNTVIVGYENLGEGGKAFSMFREALRNQVSVTEVTFSSALGACASLASMDLGVQVHGLAIKTNNAKKVAVSNSLIDMYAKCGDIKFAQSVFNEMETIDVASWNALISGYSTHGLGRQALRILDIMKDRDCKPNGLTFLGVLSGCSNAGLIDQGQECFESMIRDHGIEPCLEHYTCMVRLLGRSGQLDKAMKLIEGIPYEPSVMIWRAMLSASMNQNNEEFARRSAEEILKINPKDEATYVLVSNMYAGAKQWANVASIRKSMKEMGVKKEPGLSWIEHQGDVHYFSVGLSDHPDMKLINGMLEWLNMKATRAGYVPDRNAVLLDMDDEEKDKRLWVHSERLALAYGLVRMPSSRNRILIMKNLRICSDCHSAMKVISSIVQRDLVIRDMNRFHHFHAGVCSCGDH 821          

HSP 2 Score: 236.113 bits (601), Expect = 4.086e-63
Identity = 175/529 (33.08%), Postives = 264/529 (49.91%), Query Frame = -2

Query: 1975 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLD-LFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASI-----AGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQ 3543
            Y  +L  C  K    SAKA+H  I+KKG   DL   N L+NAY K G    A  + D MPER+ VS+  L  G   +    D + LY  + ++G   N H   + LK   +SLD       LH   VK+G   + FVG++L++ Y+ CG ++ A  VF  +  K++V W  +++ Y   G     L L   M  +    + +T    LK+   L    +    H  ++KT    D  VG  LL LY++ G   D  KVF  + + ++V WS MI    + G   EA +LF  MR   V PN+ T +SI      G  + LG++L     H  + K GF+ +I +SNALI +Y K   +    ++F E+SS N  SWN+++ G +N     +  S+F + L         TF+S L +C++L ++  G QVH   IK        V  SLID+YAK G +  A+ +F ++   D+ SW ALI+GY+      +A++  + M+ +   PN  T    LS CSN  +++ GQ
Sbjct:   52 YGAMLRRCIQKNDPISAKAIHCDILKKGSCLDLFATNILLNAYVKAGFDKDALNLFDEMPERNNVSFVTLAQGYACQ----DPIGLYSRLHREGHELNPHVFTSFLK-LFVSLDKAEICPWLHSPIVKLGYDSNAFVGAALINAYSVCGSVDSARTVFEGILCKDIVVWAGIVSCYVENGYFEDSLKLLSCMRMAGFMPNNYTFDTALKASIGLGAFDFAKGVHGQILKTCYVLDPRVGVGLLQLYTQLGDMSDAFKVFNEMPKNDVVPWSFMIARFCQNGFCNEAVDLFIRMREAFVVPNEFTLSSILNGCAIGKCSGLGEQL-----HGLVVKVGFDLDIYVSNALIDVYAKCEKMDTAVKLFAELSSKNEVSWNTVIVGYENLGEGGKAFSMFREALRNQVSVTEVTFSSALGACASLASMDLGVQVHGLAIKTNNAKKVAVSNSLIDMYAKCGDIKFAQSVFNEMETIDVASWNALISGYSTHGLGRQALRILDIMKDRDCKPNGLTFLGVLSGCSNAGLIDQGQ 570          

HSP 3 Score: 173.711 bits (439), Expect = 5.577e-43
Identity = 142/471 (30.15%), Postives = 229/471 (48.62%), Query Frame = -2

Query: 2083 VHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLF-FGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKN-GLKDNNLVGTSLIDLYAKNGFLADAELLFTQL-IERDLFSWTALIAGYAQSDCSEKA 3486
            +H  I+K G+D +  +  +L+NAY+  G    A  V + +  +D+V W  + S  V  GY  D L L   M+  G +PN +   TALKA S+ L  F F   +HG+ +K   +LD  VG  L+ LY + G M  A +VF  M K +VV W+ ++  +   G   + ++LF  M E+ +  ++FTLS++L  C        G   H LV+K G + D +V   L+D+Y+KC   +  +K+F  +   N V+W+ +I   +  G+  +A  +F       VS  + TF+S  G  A+L        VH    K     ++ +SN+LI MY K   ++    VF EM + + ASWN+L+SG        +   I   M     KP+  TF  +L  CSN   I  G +    +I++ G++      T ++ L  ++G L  A  L   +  E  +  W A+++     +  E A
Sbjct:  168 LHSPIVKLGYDSNAFVGAALINAYSVCGSVDSARTVFEGILCKDIVVWAGIVSCYVENGYFEDSLKLLSCMRMAGFMPNNYTFDTALKA-SIGLGAFDFAKGVHGQILKTCYVLDPRVGVGLLQLYTQLGDMSDAFKVFNEMPKNDVVPWSFMIARFCQNGFCNEAVDLFIRMREAFVVPNEFTLSSILNGCAIGKCSGLGEQLHGLVVKVGFDLDIYVSNALIDVYAKCEKMDTAVKLFAELSSKNEVSWNTVIVGYENLGEGGKAFSMFREALRNQVSVTEVTFSSALGACASLASMDLGVQVHGLAIKTNNAKKVAVSNSLIDMYAKCGDIKFAQSVFNEMETIDVASWNALISGYSTHGLGRQALRILDIMKDRDCKPNGLTFLGVLSGCSNAGLIDQGQECFESMIRDHGIEPCLEHYTCMVRLLGRSGQLDKAMKLIEGIPYEPSVMIWRAMLSASMNQNNEEFA 637          

HSP 4 Score: 121.709 bits (304), Expect = 1.217e-26
Identity = 80/279 (28.67%), Postives = 136/279 (48.75%), Query Frame = -2

Query: 2725 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAH------SLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMI 3540
            S IL+ CA        + +HG ++K GFD D+++ N+L++ YAK      A K+   +  ++ VSW  +  G  N G G     ++    ++ V        +AL AC+    +  G  +HG  +K      V V +SL+D+YAKCG ++ A+ VF  M+  +V SWNAL++GY+  G   + L +   M + + + +  T   VL  C+       GLI        S++   G E       C++ L  + G  +  +K+ + I  EP+++ W AM+
Sbjct:  352 SSILNGCAIGKCSGLGEQLHGLVVKVGFDLDIYVSNALIDVYAKCEKMDTAVKLFAELSSKNEVSWNTVIVGYENLGEGGKAFSMFREALRNQVSVTEVTFSSALGACASLASMDLGVQVHGLAIKTNNAKKVAVSNSLIDMYAKCGDIKFAQSVFNEMETIDVASWNALISGYSTHGLGRQALRILDIMKDRDCKPNGLTFLGVLSGCS-----NAGLIDQGQECFESMIRDHGIEPCLEHYTCMVRLLGRSGQLDKAMKLIEGIPYEPSVMIWRAML 625          

HSP 5 Score: 65.855 bits (159), Expect = 1.984e-9
Identity = 55/197 (27.92%), Postives = 87/197 (44.16%), Query Frame = -2

Query: 3022 LFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSL------VDLYAKCGQMELAERVFLFMD-KKNVVSWNALLNG 3591
            +F+E  + + +     +S  L  CAS  S+     VHG  IK      + + NSL++ YAK G   +A  V + M   DV SW AL SG    G G   L +   MK     PNG   +  L  CS +     G +  G+     ++ D  +   L      V L  + GQ++ A ++   +  + +V+ W A+L+ 
Sbjct:  436 MFREALRNQVSVTEVTFSSALGACASLASMDLGVQVHGLAIKTNNAKKVAVSNSLIDMYAKCGDIKFAQSVFNEMETIDVASWNALISGYSTHGLGRQALRILDIMKDRDCKPNGLTFLGVLSGCSNA-----GLIDQGQECFESMIRDHGIEPCLEHYTCMVRLLGRSGQLDKAMKLIEGIPYEPSVMIWRAMLSA 627          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP207_ARATH (Pentatricopeptide repeat-containing protein At3g02330 OS=Arabidopsis thaliana GN=PCMP-E90 PE=2 SV=2)

HSP 1 Score: 436.032 bits (1120), Expect = 1.577e-132
Identity = 253/825 (30.67%), Postives = 423/825 (51.27%), Query Frame = -2

Query: 1228 YSKILHDCASKGSLSSAKAVHGKIIKKGFDP-------------------------------DLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERD--------------------LFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGND-KLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKL 3543
            +S +  +CA +G+L   K  H  +I  GF P                               D+  WN ++N Y+K      A    + MP RDVVSW ++ SG +  G     ++++  M ++G+  +G      LK CS   D   G  +HG  V+VG   DV   S+L+D+YAK  +   + RVF  + +KN VSW+A++ G          L  F+ M +     S+   ++VL+SC  L  L  G   H+  +K+    D  V    LD+Y+KC   +D   +F   +  N  +++AMI    ++    +A  LF  +   G+  ++ + + +    A +        ++    K     ++ ++NA I MY K +++   +RVF EM   ++ S    + +  QNGK  +    +F  ML    +PD  TF SIL++C+   ++ +G ++H+ I+K+G+  N+ VG SLID+Y+K G + +AE + ++  +R                       SW ++I+GY   + SE A   F  M   G+TP+ FT AT L +C+N+A    G+ +H+  IK     DV++ S++VDMY+ CG + ++   F   +  D V+WN M+ GY  H +G + ++ F  M+ + ++P+ VTFI++L AC++MG +++G EYF  +   +G+ P + H + M+DILG++GK       I++M   ++ +IW T+LG C +H N+ ++ E A   L  L+P  +S Y LL+N++A  G WE V  +R  M    +KKEPGCSWV +  ++H+F   D  HP+  EIY +L
Sbjct:   51 FSFVFKECAKQGALELGKQAHAHMIISGFRPTTFVLNCLLQVYTNSRDFVSASMVFDKMPLRDVVSWNKMINGYSKSNDMFKANSFFNMMPVRDVVSWNSMLSGYLQNGESLKSIEVFVDMGREGIEFDGRTFAIILKVCSFLEDTSLGMQIHGIVVRVGCDTDVVAASALLDMYAKGKRFVESLRVFQGIPEKNSVSWSAIIAGCVQNNLLSLALKFFKEMQKVNAGVSQSIYASVLRSCAALSELRLGGQLHAHALKSDFAADGIVRTATLDMYAKCDNMQDAQILFDNSENLNRQSYNAMITGYSQEEHGFKALLLFHRLMSSGLGFDEISLSGVFRACALVKGLSEGLQIYGLAIKSSLSLDVCVANAAIDMYGKCQALAEAFRVFDEMRRRDAVSWNAIIAAHEQNGKGYET-LFLFVSMLRSRIEPDEFTFGSILKACTGG-SLGYGMEIHSSIVKSGMASNSSVGCSLIDMYSKCGMIEEAEKIHSRFFQRANVSGTMEELEKMHNKRLQEMCVSWNSIISGYVMKEQSEDAQMLFTRMMEMGITPDKFTYATVLDTCANLASAGLGKQIHAQVIKKELQSDVYICSTLVDMYSKCGDLHDSRLMFEKSLRRDFVTWNAMICGYAHHGKGEEAIQLFERMILENIKPNHVTFISILRACAHMGLIDKGLEYFYMMKRDYGLDPQLPHYSNMVDILGKSGKVKRALELIREMPFEADDVIWRTLLGVCTIHRNNVEVAEEATAALLRLDPQDSSAYTLLSNVYADAGMWEKVSDLRRNMRGFKLKKEPGCSWVELKDELHVFLVGDKAHPRWEEIYEEL 873          

HSP 2 Score: 251.136 bits (640), Expect = 8.470e-68
Identity = 173/654 (26.45%), Postives = 310/654 (47.40%), Query Frame = -2

Query: 1708 VPLFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASI--AGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGK--------------------TCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLS 3597
            V + +E  + +G    + ++ IL  C+     S    +HG +++ G D D+   ++L++ YAK   F  + +V   +PE++ VSW+A+ +G V        L  +  M+K     +     + L++C+   +L  G  LH   +K     D  V ++ +D+YAKC  M+ A+ +F   +  N  S+NA++ GY+ +    K L LF  +  S L   + +LS V ++C  +  L+ GL  + L IK+    D  V    +D+Y KC    +  +VF  ++  + V+W+A+I   ++ G+  E   LF  M    + P++ TF SI  A    +LG   Y   +H+ I K G  S   +  +LI MY K   ++   ++        + S         + K                      +    +F +M+  G  PD  T+ ++L +C+NL +   G Q+HA +IK  L+ +  + ++L+D+Y+K G L D+ L+F + + RD  +W A+I GYA     E+AI+ F  M  + + PN+ T  + L +C++M +++ G +  + M    G    +   S++VD+    G ++ A      M    D V W  +L     HR  ++V E     L + L+P + +   +LS
Sbjct:  169 VDMGREGIEFDG----RTFAIILKVCSFLEDTSLGMQIHGIVVRVGCDTDVVAASALLDMYAKGKRFVESLRVFQGIPEKNSVSWSAIIAGCVQNNLLSLALKFFKEMQKVNAGVSQSIYASVLRSCAALSELRLGGQLHAHALKSDFAADGIVRTATLDMYAKCDNMQDAQILFDNSENLNRQSYNAMITGYSQEEHGFKALLLFHRLMSSGLGFDEISLSGVFRACALVKGLSEGLQIYGLAIKSSLSLDVCVANAAIDMYGKCQALAEAFRVFDEMRRRDAVSWNAIIAAHEQNGKGYETLFLFVSMLRSRIEPDEFTFGSILKACTGGSLG---YGMEIHSSIVKSGMASNSSVGCSLIDMYSKCGMIEEAEKIHSRFFQRANVSGTMEELEKMHNKRLQEMCVSWNSIISGYVMKEQSEDAQMLFTRMMEMGITPDKFTYATVLDTCANLASAGLGKQIHAQVIKKELQSDVYICSTLVDMYSKCGDLHDSRLMFEKSLRRDFVTWNAMICGYAHHGKGEEAIQLFERMILENIKPNHVTFISILRACAHMGLIDKGLEYFYMMKRDYGLDPQLPHYSNMVDILGKSGKVKRALELIREMPFEADDVIWRTLLGVCTIHRNNVEVAEEATAALLR-LDPQDSSAYTLLS 814          

HSP 3 Score: 224.557 bits (571), Expect = 4.214e-59
Identity = 173/697 (24.82%), Postives = 323/697 (46.34%), Query Frame = -2

Query: 1444 LHLWNSLVN---------AYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAE---AAFLGMVSTDT-----------------VSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFE 3447
            LH+  S+V+         +Y +   F Y    L+++      +++ +F     +G        +  M   G  P    L   L+  + S D    +++  +      L DV   + +++ Y+K   M  A   F  M  ++VVSWN++L+GY   G++ K + +F  M    +     T + +LK C++L + + G+  H +V++ GC+ D      LLD+Y+K     ++L+VF+ I E N V+WSA+I    +   +  A + F  M+      +Q  +AS+    A L +      +HA   K  F ++ I+  A + MY K  ++Q+   +F    + N  S+N++++G    +   +   +FH++++ G   D  + + + R+C+ +  +  G Q++   IK+ L  +  V  + ID+Y K   LA+A  +F ++  RD  SW A+IA + Q+    + +  F  M R  + P+ FT  + L +C+    L  G  +HS  +K+G + +  V  S++DMY+ CG IEEAE   + F    +                    VSWN ++ GY+   Q       F  M++ G+ PD+ T+  VL  C+ +     GK+    +     +   +  C+ ++D+  + G  ++    + +  +  + + W  ++     HG    GE A + LFE
Sbjct:    8 LHMTRSVVSFNRCLTEKISYRRVPSFSYFTDFLNQVNSVSTTNFSFVFKECAKQGALELGKQAHAHMIISGFRPTTFVLNCLLQVYTNSRDFVSASMVFDKMP----LRDVVSWNKMINGYSKSNDMFKANSFFNMMPVRDVVSWNSMLSGYLQNGESLKSIEVFVDMGREGIEFDGRTFAIILKVCSFLEDTSLGMQIHGIVVRVGCDTDVVAASALLDMYAKGKRFVESLRVFQGIPEKNSVSWSAIIAGCVQNNLLSLALKFFKEMQKVNAGVSQSIYASVLRSCAALSELRLGGQLHAHALKSDFAADGIVRTATLDMYAKCDNMQDAQILFDNSENLNRQSYNAMITGYSQEEHGFKALLLFHRLMSSGLGFDEISLSGVFRACALVKGLSEGLQIYGLAIKSSLSLDVCVANAAIDMYGKCQALAEAFRVFDEMRRRDAVSWNAIIAAHEQNGKGYETLFLFVSMLRSRIEPDEFTFGSILKACTG-GSLGYGMEIHSSIVKSGMASNSSVGCSLIDMYSKCGMIEEAEKIHSRFFQRANVSGTMEELEKMHNKRLQEMCVSWNSIISGYVMKEQSEDAQMLFTRMMEMGITPDKFTYATVLDTCANLASAGLGKQIHAQVIKK-ELQSDVYICSTLVDMYSKCGDLHD-SRLMFEKSLRRDFVTWNAMICGYAHHGK---GEEAIQ-LFE 693          

HSP 4 Score: 200.675 bits (509), Expect = 2.223e-51
Identity = 140/520 (26.92%), Postives = 252/520 (48.46%), Query Frame = -2

Query: 2116 SLKVPLFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKR-IQEPNI-------------------VAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVF-KEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKN-GLKDNNLVGTSLIDLYAKNGFLADA-ELLFTQLIERDLFSWTALIA 3606
            SL +  FKE  K+       +Y+ +L  CA+   L     +H   +K  F  D  +  + ++ YAK      A  + D     +  S+ A+ +G   E +G+  L L+  +   G+  +   L    +AC+L   L  G  ++G  +K  + LDV V ++ +D+Y KC  +  A RVF  M +++ VSWNA++  +   G   + L LF  M  S +   +FT  ++LK+CT   +L +G+  HS ++K+G   +  VGC L+D+YSKCG+ E+  K+  R  Q  N+                   V+W+++I     + Q ++A  LF+ M   G++P++ T+A++    ANL      + +HA + K   +S++ + + L+ MY K   + +   +F K +          +   + +GK  +    +F +M+ E  KP+  TF SILR+C+++  I  G +    + ++ GL       ++++D+  K+G +  A EL+     E D   W  L+ 
Sbjct:  263 SLALKFFKEMQKVNAGVSQSIYASVLRSCAALSELRLGGQLHAHALKSDFAADGIVRTATLDMYAKCDNMQDAQILFDNSENLNRQSYNAMITGYSQEEHGFKALLLFHRLMSSGLGFDEISLSGVFRACALVKGLSEGLQIYGLAIKSSLSLDVCVANAAIDMYGKCQALAEAFRVFDEMRRRDAVSWNAIIAAHEQNGKGYETLFLFVSMLRSRIEPDEFTFGSILKACTG-GSLGYGMEIHSSIVKSGMASNSSVGCSLIDMYSKCGMIEEAEKIHSRFFQRANVSGTMEELEKMHNKRLQEMCVSWNSIISGYVMKEQSEDAQMLFTRMMEMGITPDKFTYATVLDTCANLASAGLGKQIHAQVIKKELQSDVYICSTLVDMYSKCGDLHDSRLMFEKSLRRDFVTWNAMICGYAHHGKG-EEAIQLFERMILENIKPNHVTFISILRACAHMGLIDKGLEYFYMMKRDYGLDPQLPHYSNMVDILGKSGKVKRALELIREMPFEADDVIWRTLLG 780          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP341_ARATH (Pentatricopeptide repeat-containing protein At4g30700 OS=Arabidopsis thaliana GN=DYW9 PE=2 SV=1)

HSP 1 Score: 431.795 bits (1109), Expect = 3.410e-132
Identity = 242/755 (32.05%), Postives = 400/755 (52.98%), Query Frame = -2

Query:  922 HGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTES-ELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELF-SLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3180
            H + +  G   D+ + + L    +  G +  A  +FL + + +V  +N L+ G+++       L++F  + +S +L+ +  T +  + + +   +   G + H   +  GC+ +  +G  ++ +Y K    ED  KVF R+ E + + W+ MI    +     E+ ++F  L+       +  T   I    A L +      +H+   K G  S   +    I++Y K   ++ G  +F+E    +  ++N+++ G  +    +   S+F +++  G +    T  S++    +L+ I   + +H + +K+    +  V T+L  +Y+K   +  A  LF +  E+ L SW A+I+GY Q+  +E AI  F  MQ+   +PN  T+   LS+C+ +  L  G+ +H +         ++VS++++ MYA CG I EA   F  M   + V+WN M+ GY  H QG + L  F  ML+ G+ P  VTF+ VL ACS+ G V EG E F+S+ + +G  P++KH ACM+DILGRAG       FI+ M +     +WET+LG CR+H +  L  + +EKLFEL+P    +++LL+NI +A   +     VR       + K PG + + +    H+F S D  HP+  EIY KL++L  K   AGY P+T+  L+DV ++E+   +  H+ERLA+AF LI+  P   IRI KNL +C DCH   KL+S + ++ IVVRDAN FHHFK G+CSC DY
Sbjct:   40 HAQIILHGFRNDISLLTKLTQRLSDLGAIYYARDIFLSVQRPDVFLFNVLMRGFSVNESPHSSLSVFAHLRKSTDLKPNSSTYAFAISAASGFRDDRAGRVIHGQAVVDGCDSELLLGSNIVKMYFKFWRVEDARKVFDRMPEKDTILWNTMISGYRKNEMYVESIQVFRDLINESCTRLDTTTLLDILPAVAELQELRLGMQIHSLATKTGCYSHDYVLTGFISLYSKCGKIKMGSALFREFRKPDIVAYNAMIHGYTSNGETELSLSLFKELMLSGARLRSSTLVSLVPVSGHLMLI---YAIHGYCLKSNFLSHASVSTALTTVYSKLNEIESARKLFDESPEKSLPSWNAMISGYTQNGLTEDAISLFREMQKSEFSPNPVTITCILSACAQLGALSLGKWVHDLVRSTDFESSIYVSTALIGMYAKCGSIAEARRLFDLMTKKNEVTWNTMISGYGLHGQGQEALNIFYEMLNSGITPTPVTFLCVLYACSHAGLVKEGDEIFNSMIHRYGFEPSVKHYACMVDILGRAGHLQRALQFIEAMSIEPGSSVWETLLGACRIHKDTNLARTVSEKLFELDPDNVGYHVLLSNIHSADRNYPQAATVRQTAKKRKLAKAPGYTLIEIGETPHVFTSGDQSHPQVKEIYEKLEKLEGKMREAGYQPETELALHDVEEEERELMVKVHSERLAIAFGLIATEPGTEIRIIKNLRVCLDCHTVTKLISKITERVIVVRDANRFHHFKDGVCSCGDY 791          

HSP 2 Score: 220.705 bits (561), Expect = 3.147e-58
Identity = 167/607 (27.51%), Postives = 286/607 (47.12%), Query Frame = -2

Query: 1708 SLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGL-VNEGYGYDVLDLYCSMKKDGVL-PNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGM-TESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVS--WNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLS 3507
            S+S     H +II  GF  D+ L   L    +  G   YA  +   +   DV  +  L  G  VNE   +  L ++  ++K   L PN      A+ A S   D   G ++HG+ V  G   ++ +GS++V +Y K  ++E A +VF  M +K+ + WN +++GY       + + +F+ +  ES  R    TL ++L +   L  L  G+  HSL  KTGC   ++V    + LYSKCG  +    +F+  ++P+IVA++AMI      G+ + +  LF  +   G      T  S+  V+ +L   +    +H    K  F S   +S AL  +Y K+  +++  ++F E    +  S    +   +QNG T D   S+F +M    F P+  T T IL +C+ L  +  G  VH  +     + +  V T+LI +YAK G +A+A  LF  + +++  +W  +I+GY      ++A+  F  M   G+TP   T    L +CS+  +++ G ++ +SM  + G    V   + +VD+    G ++ A   F+  +S +  S  W  +L     H+           + +  L+PD V +  +LS
Sbjct:   32 SISHLAQTHAQIILHGFRNDISLLTKLTQRLSDLGAIYYARDIFLSVQRPDVFLFNVLMRGFSVNES-PHSSLSVFAHLRKSTDLKPNSSTYAFAISAASGFRDDRAGRVIHGQAVVDGCDSELLLGSNIVKMYFKFWRVEDARKVFDRMPEKDTILWNTMISGYRKNEMYVESIQVFRDLINESCTRLDTTTLLDILPAVAELQELRLGMQIHSLATKTGCYSHDYVLTGFISLYSKCGKIKMGSALFREFRKPDIVAYNAMIHGYTSNGETELSLSLFKELMLSGARLRSSTLVSLVPVSGHL---MLIYAIHGYCLKSNFLSHASVSTALTTVYSKLNEIESARKLFDESPEKSLPSWNAMISGYTQNGLTED-AISLFREMQKSEFSPNPVTITCILSACAQLGALSLGKWVHDLVRSTDFESSIYVSTALIGMYAKCGSIAEARRLFDLMTKKNEVTWNTMISGYGLHGQGQEALNIFYEMLNSGITPTPVTFLCVLYACSHAGLVKEGDEIFNSMIHRYGFEPSVKHYACMVDILGRAGHLQRA-LQFIEAMSIEPGSSVWETLLGACRIHKDTNLARTVSEKLFE--LDPDNVGYHVLLS 630          

HSP 3 Score: 122.479 bits (306), Expect = 7.597e-27
Identity = 91/339 (26.84%), Postives = 154/339 (45.43%), Query Frame = -2

Query: 2536 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTF------ASIAGVAANLGDRLYCEGVHACIFKHGFESEI 3534
            IL   A    L     +H    K G     ++    ++ Y+K G       +     + D+V++ A+  G  + G     L L+  +   G       LV+ L   S  L L +   +HG  +K   L    V ++L  +Y+K  ++E A ++F    +K++ SWNA+++GY   G     ++LF+ M +SE   +  T++ +L +C  L  L+ G   H LV  T  E   +V   L+ +Y+KCG   +  ++F  + + N V W+ MI      GQ QEA  +F  M   G++P   TF       S AG+    GD ++   +H    ++GFE  +
Sbjct:  227 ILPAVAELQELRLGMQIHSLATKTGCYSHDYVLTGFISLYSKCGKIKMGSALFREFRKPDIVAYNAMIHGYTSNGETELSLSLFKELMLSGARLRSSTLVS-LVPVSGHLMLIYA--IHGYCLKSNFLSHASVSTALTTVYSKLNEIESARKLFDESPEKSLPSWNAMISGYTQNGLTEDAISLFREMQKSEFSPNPVTITCILSACAQLGALSLGKWVHDLVRSTDFESSIYVSTALIGMYAKCGSIAEARRLFDLMTKKNEVTWNTMISGYGLHGQGQEALNIFYEMLNSGITPTPVTFLCVLYACSHAGLVKE-GDEIFNSMIH----RYGFEPSV 557          

HSP 4 Score: 70.8626 bits (172), Expect = 6.080e-11
Identity = 55/220 (25.00%), Postives = 99/220 (45.00%), Query Frame = -2

Query: 2965 VPLFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLS-----LDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVS----WNALLNGYALKGDAGKILNLFQGMTE 3597
            + LF+E  K E +      + IL  CA  G+LS  K VH  +    F+  +++  +L+  YAK G    A ++ D M +++ V+W  + SG    G G + L+++  M   G+ P     +  L ACS +      D  F +++H    + G    V   + +VD+  + G +   +R   F++  ++      W  LL    +  D     NL + ++E
Sbjct:  405 ISLFREMQKSEFSPNPVTITCILSACAQLGALSLGKWVHDLVRSTDFESSIYVSTALIGMYAKCGSIAEARRLFDLMTKKNEVTWNTMISGYGLHGQGQEALNIFYEMLNSGITPTPVTFLCVLYACSHAGLVKEGDEIFNSMIH----RYGFEPSVKHYACMVDILGRAGHL---QRALQFIEAMSIEPGSSVWETLLGACRIHKDT----NLARTVSE 613          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP357_ARATH (Pentatricopeptide repeat-containing protein At4g39530 OS=Arabidopsis thaliana GN=PCMP-E52 PE=3 SV=1)

HSP 1 Score: 432.18 bits (1110), Expect = 6.635e-132
Identity = 256/792 (32.32%), Postives = 425/792 (53.66%), Query Frame = -2

Query: 1216 GAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSM---KKDGVLPNGHCLVTALKACSLSLD---LFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKT---CDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELV 3564
            G +  + ++++L   AS   L     VHG+II  G + D +L N L+N Y++ G   YA KV ++MPER++VSW+ + S   + G   + L ++      +KD   PN + L + ++ACS  LD    +    L    VK G   DV+VG+ L+D Y K G ++ A  VF  + +K+ V+W  +++G    G +   L LF  + E  +    + LS VL +C+ L  L  G   H+ +++ G E D  +   L+D Y KCG      K+F  +   NI++W+ ++    +    +EA ELF+ M   G+ P+ +  +SI    A+L    +   VHA   K    ++  ++N+LI MY K   + +  +VF   ++ +   +N+++ G     T        +IF  M     +P + TF S+LR+ ++L ++    Q+H  + K GL  +   G++LID+Y+    L D+ L+F ++  +DL  W ++ AGY Q   +E+A+  F  +Q     P+ FT A  +++  N+A ++ GQ  H   +K G   + +++++++DMYA CG  E+A  AF    S D V WN ++  Y  H +G K L+    M+ +G+EP+ +TF+ VLSACS+ G V +G + F+ +   FGI P  +H  CM+ +LGRAG+ N+    I+ M      ++W ++L  C   GN +L E AAE     +P  +  + +L+NI+A+KG W + ++VR  M   GV KEPG SW+ +N +VHIF S D  H K  +IY  L +L+
Sbjct:   40 GIRGRREFARLLQLRASDDLLHYQNVVHGQIIVWGLELDTYLSNILINLYSRAGGMVYARKVFEKMPERNLVSWSTMVSACNHHGIYEESLVVFLEFWRTRKDS--PNEYILSSFIQACS-GLDGRGRWMVFQLQSFLVKSGFDRDVYVGTLLIDFYLKDGNIDYARLVFDALPEKSTVTWTTMISGCVKMGRSYVSLQLFYQLMEDNVVPDGYILSTVLSACSILPFLEGGKQIHAHILRYGLEMDASLMNVLIDSYVKCGRVIAAHKLFNGMPNKNIISWTTLLSGYKQNALHKEAMELFTSMSKFGLKPDMYACSSILTSCASLHALGFGTQVHAYTIKANLGNDSYVTNSLIDMYAKCDCLTDARKVFDIFAAADVVLFNAMIEGYSRLGTQWELHEALNIFRDMRFRLIRPSLLTFVSLLRASASLTSLGLSKQIHGLMFKYGLNLDIFAGSALIDVYSNCYCLKDSRLVFDEMKVKDLVIWNSMFAGYVQQSENEEALNLFLELQLSRERPDEFTFANMVTAAGNLASVQLGQEFHCQLLKRGLECNPYITNALLDMYAKCGSPEDAHKAFDSAASRDVVCWNSVISSYANHGEGKKALQMLEKMMSEGIEPNYITFVGVLSACSHAGLVEDGLKQFELMLR-FGIEPETEHYVCMVSLLGRAGRLNKARELIEKMPTKPAAIVWRSLLSGCAKAGNVELAEHAAEMAILSDPKDSGSFTMLSNIYASKGMWTEAKKVRERMKVEGVVKEPGRSWIGINKEVHIFLSKDKSHCKANQIYEVLDDLL 827          

HSP 2 Score: 155.606 bits (392), Expect = 3.606e-37
Identity = 98/336 (29.17%), Postives = 167/336 (49.70%), Query Frame = -2

Query: 2599 VPLFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDV---LDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLG 3597
            + LF   +K      +   S IL  CAS  +L     VH   IK     D ++ NSL++ YAK      A KV D     DVV + A+  G    G  +++   L+++  M+   + P+    V+ L+A +    L     +HG   K G+ LD+F GS+L+D+Y+ C  ++ +  VF  M  K++V WN++  GY  + +  + LNLF  +  S  R  +FT +N++ +   L ++  G   H  ++K G E + ++   LLD+Y+KCG  ED  K F      ++V W+++I      G+ ++A ++   M  +G+ PN  TF  +    ++ G
Sbjct:  335 MELFTSMSKFGLKPDMYACSSILTSCASLHALGFGTQVHAYTIKANLGNDSYVTNSLIDMYAKCDCLTDARKVFDIFAAADVVLFNAMIEGYSRLGTQWELHEALNIFRDMRFRLIRPSLLTFVSLLRASASLTSLGLSKQIHGLMFKYGLNLDIFAGSALIDVYSNCYCLKDSRLVFDEMKVKDLVIWNSMFAGYVQQSENEEALNLFLELQLSRERPDEFTFANMVTAAGNLASVQLGQEFHCQLLKRGLECNPYITNALLDMYAKCGSPEDAHKAFDSAASRDVVCWNSVISSYANHGEGKKALQMLEKMMSEGIEPNYITFVGVLSACSHAG 670          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP251_ARATH (Putative pentatricopeptide repeat-containing protein At3g23330 OS=Arabidopsis thaliana GN=PCMP-H32 PE=3 SV=1)

HSP 1 Score: 426.787 bits (1096), Expect = 2.800e-131
Identity = 230/716 (32.12%), Postives = 392/716 (54.75%), Query Frame = -2

Query:  922 LRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKT-GCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKI------------------RSVQNG------------------WRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 2958
            +  SK  +  ++K+ T + + +     H+  I+T    H       ++ +Y+   L  + L +FK ++ P ++AW ++I C  +Q    +A   F  MR  G  P+ + F S+      + D  + E VH  I + G + ++   NAL+ MY K+                  R+  +G                   RVF+ M   +  S+N++++G       +    +  +M     KPD  T +S+L   S  +++  G ++H ++I+ G+  +  +G+SL+D+YAK+  + D+E +F++L  RD  SW +L+AGY Q+    +A++ F  M    V P     ++ + +C+++A L  G+ LH   ++ G   ++F++S++VDMY+ CG I+ A   F  M   D VSW  ++ G+  H  G + +  F  M  +G++P++V F+AVL+ACS++G V+E   YF+S+T V+G+   ++H A + D+LGRAGK  E  +FI  M V     +W T+L +C +H N +L E  AEK+F ++      Y+L+ N++A+ GRW+++ ++R  M   G++K+P CSW+ +  + H F S D  HP   +I   LK ++E+    GYV  T  +L+DV ++ K E LF H+ERLA+AF +I+  P  TIR+ KN+ IC DCH  +K +S + ++EI+VRD + FHHF  G CSC DY
Sbjct:    1 MSSSKALIKTLIKNPTRIKSKSQAKQLHAQFIRTQSLSHTS--ASIVISIYTNLKLLHEALLLFKTLKSPPVLAWKSVIRCFTDQSLFSKALASFVEMRASGRCPDHNVFPSVLKSCTMMMDLRFGESVHGFIVRLGMDCDLYTGNALMNMYAKLLGMGSKISVGNVFDEMPQRTSNSGDEDVKAETCIMPFGIDSVRRVFEVMPRKDVVSYNTIIAGYAQSGMYEDALRMVREMGTTDLKPDSFTLSSVLPIFSEYVDVIKGKEIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSRLYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVLRGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNVLDEVSWTAIIMGHALHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVGLVDEAWGYFNSMTKVYGLNQELEHYAAVADLLGRAGKLEEAYNFISKMCVEPTGSVWSTLLSSCSVHKNLELAEKVAEKIFTVDSENMGAYVLMCNMYASNGRWKEMAKLRLRMRKKGLRKKPACSWIEMKNKTHGFVSGDRSHPSMDKINEFLKAVMEQMEKEGYVADTSGVLHDVDEEHKRELLFGHSERLAVAFGIINTEPGTTIRVTKNIRICTDCHVAIKFISKITEREIIVRDNSRFHHFNRGNCSCGDY 714          

HSP 2 Score: 174.096 bits (440), Expect = 1.928e-43
Identity = 104/352 (29.55%), Postives = 172/352 (48.86%), Query Frame = -2

Query: 2599 LYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAK--------------------------RGVFGYAC----------KVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLG 3546
            ++  +L  C     L   ++VHG I++ G D DL+  N+L+N YAK                            V    C          +V + MP +DVVS+  + +G    G   D L +   M    + P+   L + L   S  +D+  G  +HG  ++ G+  DV++GSSLVD+YAK  ++E +ERVF  +  ++ +SWN+L+ GY   G   + L LF+ M  ++++      S+V+ +C +L  L  G   H  V++ G   + F+   L+D+YSKCG  +   K+F R+   + V+W+A+I      G   EA  LF  M+ +GV PNQ  F ++    +++G
Sbjct:  107 VFPSVLKSCTMMMDLRFGESVHGFIVRLGMDCDLYTGNALMNMYAKLLGMGSKISVGNVFDEMPQRTSNSGDEDVKAETCIMPFGIDSVRRVFEVMPRKDVVSYNTIIAGYAQSGMYEDALRMVREMGTTDLKPDSFTLSSVLPIFSEYVDVIKGKEIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSRLYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVLRGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNVLDEVSWTAIIMGHALHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVG 458          

HSP 3 Score: 161.384 bits (407), Expect = 2.331e-39
Identity = 113/452 (25.00%), Postives = 219/452 (48.45%), Query Frame = -2

Query: 2116 VVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQM------------------------------------ELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSN--LLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQL-IERDLFSWTALIA 3354
            V++W ++     ++      L  +  M+  G  P+ +   + LK+C++ +DL FG  +HG  V++G+  D++ G++L+++YAK   M                                    +   RVF  M +K+VVS+N ++ GYA  G     L + + M  ++L+   FTLS+VL   +  V++  G   H  VI+ G + D ++G  L+D+Y+K    ED+ +VF R+   + ++W++++    + G+  EA  LF  M    V P    F+S+    A+L      + +H  + + GF S I +++AL+ MY K  +++   ++F  M+  +  SW +++ G           S+F +M  +G KP+   F ++L +CS+  L++  +G+  ++     GL        ++ DL  + G L +A    +++ +E     W+ L++
Sbjct:   70 VLAWKSVIRCFTDQSLFSKALASFVEMRASGRCPDHNVFPSVLKSCTMMMDLRFGESVHGFIVRLGMDCDLYTGNALMNMYAKLLGMGSKISVGNVFDEMPQRTSNSGDEDVKAETCIMPFGIDSVRRVFEVMPRKDVVSYNTIIAGYAQSGMYEDALRMVREMGTTDLKPDSFTLSSVLPIFSEYVDVIKGKEIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSRLYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVLRGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNVLDEVSWTAIIMGHALHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVGLVDEAWGY-FNSMTKVYGLNQELEHYAAVADLLGRAGKLEEAYNFISKMCVEPTGSVWSTLLS 520          

HSP 4 Score: 132.88 bits (333), Expect = 2.887e-30
Identity = 77/260 (29.62%), Postives = 135/260 (51.92%), Query Frame = -2

Query: 2725 AKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTY--LVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRI-QEPNIVAWSAMI 3495
             K +HG +I+KG D D+++ +SLV+ YAK      + +V  R+  RD +SW +L +G V  G   + L L+  M    V P      + + AC+    L  G  LHG  ++ G   ++F+ S+LVD+Y+KCG ++ A ++F  M+  + VSW A++ G+AL G   + ++LF+ M    ++ ++     VL +C++  LV+  WG   +S+    G   +      + DL  + G  E+      ++  EP    WS ++
Sbjct:  261 GKEIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSRLYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVLRGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNVLDEVSWTAIIMGHALHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVGLVDEAWGYF-NSMTKVYGLNQELEHYAAVADLLGRAGKLEEAYNFISKMCVEPTGSVWSTLL 519          

HSP 5 Score: 80.1073 bits (196), Expect = 7.414e-14
Identity = 69/245 (28.16%), Postives = 110/245 (44.90%), Query Frame = -2

Query: 2929 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSL------------SLDLFFG---TLLHGEGV-----KVGVLLDVF----------VGSSLVDLYAKCG---QMELAERV---FLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELR----CSKFTLSN 3543
            +S ++  CA   +L   K +HG +++ GF  ++ + ++LV+ Y+K G    A K+ DRM   D VSWTA+  G    G+G++ + L+  MK+ GV PN    V  L ACS             S+   +G    L H   V     + G L + +           GS    L + C     +ELAE+V      +D +N+ ++  + N YA  G   ++  L   M +  LR    CS   + N
Sbjct:  346 FSSVIPACAHLATLHLGKQLHGYVLRGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNVLDEVSWTAIIMGHALHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVGLVDEAWGYFNSMTKVYGLNQELEHYAAVADLLGRAGKLEEAYNFISKMCVEPTGSVWSTLLSSCSVHKNLELAEKVAEKIFTVDSENMGAYVLMCNMYASNGRWKEMAKLRLRMRKKGLRKKPACSWIEMKN 590          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP220_ARATH (Pentatricopeptide repeat-containing protein At3g09040, mitochondrial OS=Arabidopsis thaliana GN=PCMP-E88 PE=2 SV=1)

HSP 1 Score: 435.647 bits (1119), Expect = 2.932e-131
Identity = 242/762 (31.76%), Postives = 412/762 (54.07%), Query Frame = -2

Query: 1213 VHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXX-XXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGL-KDNNLVGTSLIDLYAKNGFLADAELLFTQLIE-RDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVE 3486
            V  ++  +G  PD   + +++N Y + G    A  +   M   DVV+W  + SG    G     ++ + +M+K  V      L + L A  +  +L  G ++H E +K+G+  +++VGSSLV +Y+KC +ME A +VF  +++KN V WNA++ GYA  G++ K++ LF  M  S      FT +++L +C    +L  G   HS++IK     + FVG  L+D+Y+KCG  ED  ++F+R+ + + V W+ +I    +     EA +LF  M   G+  +    AS      ++      + VH    K G + ++   ++LI MY K   +++  +VF  +      +    +   SQN    +    +F +ML  G  P   TF +I+ +C    ++  G Q H  I K G   +   +G SL+ +Y  +  + +A  LF++L   + +  WT +++G++Q+   E+A+K +  M+  GV P+  T  T L  CS ++ L  G+ +HS+     +  D   S++++DMYA CG ++ +   F  M   ++ VSWN ++ GY ++      L+ F  M    + PDE+TF+ VL+ACS+ G V++G++ F+ +   +GI   + H ACM+D+LGR G   E + FI+   +  +  +W ++LG CR+HG+D  GE +AEKL EL P  +S Y+LL+NI+A++G WE    +R +M   GVKK PG SW+ V  + HIF + D  H + G+I + L++L +
Sbjct:  248 VFERMRDEGHRPDHLAFVTVINTYIRLGKLKDARLLFGEMSSPDVVAWNVMISGHGKRGCETVAIEYFFNMRKSSVKSTRSTLGSVLSAIGIVANLDLGLVVHAEAIKLGLASNIYVGSSLVSMYSKCEKMEAAAKVFEALEEKNDVFWNAMIRGYAHNGESHKVMELFMDMKSSGYNIDDFTFTSLLSTCAASHDLEMGSQFHSIIIKKKLAKNLFVGNALVDMYAKCGALEDARQIFERMCDRDNVTWNTIIGSYVQDENESEAFDLFKRMNLCGIVSDGACLASTLKACTHVHGLYQGKQVHCLSVKCGLDRDLHTGSSLIDMYSKCGIIKDARKVFSSLPEWSVVSMNALIAGYSQN--NLEEAVVLFQEMLTRGVNPSEITFATIVEACHKPESLTLGTQFHGQITKRGFSSEGEYLGISLLGMYMNSRGMTEACALFSELSSPKSIVLWTGMMSGHSQNGFYEEALKFYKEMRHDGVLPDQATFVTVLRVCSVLSSLREGRAIHSLIFHLAHDLDELTSNTLIDMYAKCGDMKGSSQVFDEMRRRSNVVSWNSLINGYAKNGYAEDALKIFDSMRQSHIMPDEITFLGVLTACSHAGKVSDGRKIFEMMIGQYGIEARVDHVACMVDLLGRWGYLQEADDFIEAQNLKPDARLWSSLLGACRIHGDDIRGEISAEKLIELEPQNSSAYVLLSNIYASQGCWEKANALRKVMRDRGVKKVPGYSWIDVEQRTHIFAAGDKSHSEIGKIEMFLEDLYD 1007          

HSP 2 Score: 268.47 bits (685), Expect = 4.386e-73
Identity = 179/622 (28.78%), Postives = 301/622 (48.39%), Query Frame = -2

Query: 1651 SLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASI---AGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLT 3507
            +L   KAVH K +  G D +  L N++V+ YAK     YA K  D + E+DV +W ++ S   + G    VL  + S+ ++ + PN       L  C+   ++ FG  +H   +K+G+  + + G +LVD+YAKC ++  A RVF ++   N V W  L +GY   G   + + +F+ M +                                    G   D      +++ Y + G  +D   +F  +  P++VAW+ MI    ++G    A E F  MR   V   + T  S+    G+ ANL   L    VHA   K G  S I + ++L++MY K   ++   +VF+ +   N   WN+++ G  +     +   +F  M + G+  D  TFTS+L +C+   +++ G Q H+ IIK  L  N  VG +L+D+YAK G L DA  +F ++ +RD  +W  +I  Y Q +   +A   F  M   G+  +   LA+ L +C+++  L  G+ +H +++K G   D+   SS++DMY+ CG I++A   F  +     VS N ++ GY Q+     V+  F+ ML +G+ P E+TF  ++ AC     +  G ++   +T
Sbjct:   75 ALRIGKAVHSKSLILGIDSEGRLGNAIVDLYAKCAQVSYAEKQFDFL-EKDVTAWNSMLSMYSSIGKPGKVLRSFVSLFENQIFPNKFTFSIVLSTCARETNVEFGRQIHCSMIKMGLERNSYCGGALVDMYAKCDRISDARRVFEWIVDPNTVCWTCLFSGYVKAGLPEEAVLVFERMRDE-----------------------------------GHRPDHLAFVTVINTYIRLGKLKDARLLFGEMSSPDVVAWNVMISGHGKRGCETVAIEYFFNMRKSSVKSTRSTLGSVLSAIGIVANLDLGLV---VHAEAIKLGLASNIYVGSSLVSMYSKCEKMEAAAKVFEALEEKNDVFWNAMIRGYAHNGESHKVMELFMDMKSSGYNIDDFTFTSLLSTCAASHDLEMGSQFHSIIIKKKLAKNLFVGNALVDMYAKCGALEDARQIFERMCDRDNVTWNTIIGSYVQDENESEAFDLFKRMNLCGIVSDGACLASTLKACTHVHGLYQGKQVHCLSVKCGLDRDLHTGSSLIDMYSKCGIIKDARKVFSSLPEWSVVSMNALIAGYSQNNLEEAVV-LFQEMLTRGVNPSEITFATIVEACHKPESLTLGTQFHGQIT 656          

HSP 3 Score: 244.588 bits (623), Expect = 3.638e-65
Identity = 177/616 (28.73%), Postives = 292/616 (47.40%), Query Frame = -2

Query: 1390 LSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGD----RLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDR-GPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDM-KVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFE--LNPGIASHYILLANIFAA 3213
            L+L L  G  +H + + +G+  +  +G+++VDLYAKC Q+  AE+ F F++K +V +WN++L+ Y+  G  GK+L  F  + E+++  +KFT S VL +C    N+ +G   H  +IK G E + + G  L+D+Y+KC    D  +VF+ I +PN V W+ +     + G  +EA  +F  MR +G  P+   F ++      LG     RL        +F      +++  N +I+ + K                                + C+      F  M     K    T  S+L +   + N+  G  VHA  IK GL  N  VG+SL+ +Y+K   +  A  +F  L E++   W A+I GYA +  S K ++ F  M+  G   ++FT  + LS+C+    LE G   HS+ IK   + ++FV +++VDMYA CG +E+A   F  M   D V+WN ++  Y+Q     +  + F+ M   G+  D     + L AC+++  + +GK+    L+   G+   +   + +ID+  + G        IKD  KV S+   W  V     + G  +     A  LF+  L  G+    I  A I  A
Sbjct:   72 LALALRIGKAVHSKSLILGIDSEGRLGNAIVDLYAKCAQVSYAEKQFDFLEK-DVTAWNSMLSMYSSIGKPGKVLRSFVSLFENQIFPNKFTFSIVLSTCARETNVEFGRQIHCSMIKMGLERNSYCGGALVDMYAKCDRISDARRVFEWIVDPNTVCWTCLFSGYVKAGLPEEAVLVFERMRDEGHRPDHLAFVTVINTYIRLGKLKDARL--------LFGEMSSPDVVAWNVMISGHGK--------------------------------RGCETVAIEYFFNMRKSSVKSTRSTLGSVLSAIGIVANLDLGLVVHAEAIKLGLASNIYVGSSLVSMYSKCEKMEAAAKVFEALEEKNDVFWNAMIRGYAHNGESHKVMELFMDMKSSGYNIDDFTFTSLLSTCAASHDLEMGSQFHSIIIKKKLAKNLFVGNALVDMYAKCGALEDARQIFERMCDRDNVTWNTIIGSYVQDENESEAFDLFKRMNLCGIVSDGACLASTLKACTHVHGLYQGKQ-VHCLSVKCGLDRDLHTGSSLIDMYSKCG-------IIKDARKVFSSLPEWSVVSMNALIAGYSQNNLEEAVVLFQEMLTRGVNPSEITFATIVEA 638          

HSP 4 Score: 202.216 bits (513), Expect = 1.696e-51
Identity = 139/483 (28.78%), Postives = 243/483 (50.31%), Query Frame = -2

Query: 2113 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHD-EFVGCCLLDLYSKCGLAEDTLKVFKRIQEP-NIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXX--XXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHII-KNGLKDNNLVGTSLIDLYAKNGFLADA-ELLFTQLIERDLFSWTALIAG 3543
            ++ +L  CA+   L      H  IIKK    +L + N+LV+ YAK G    A ++ +RM +RD V+W  +    V +    +  DL+  M   G++ +G CL + LKAC+    L+ G  +H   VK G+  D+  GSSL+D+Y+KCG ++ A +VF  + + +VVS NAL+ GY+ + +  + + LFQ M    +  S+ T + ++++C    +LT G   H  + K G   + E++G  LL +Y       +   +F  +  P +IV W+ M+    + G  +EA + +  MR  GV P+Q TF ++  V + L        +H+ IF    + + + SN LI MY K   ++   +VF EM   ++     + +   ++NG   D    IF  M      PD  TF  +L +CS+   +  G ++   +I + G++        ++DL  + G+L +A + +  Q ++ D   W++L+  
Sbjct:  431 FTSLLSTCAASHDLEMGSQFHSIIIKKKLAKNLFVGNALVDMYAKCGALEDARQIFERMCDRDNVTWNTIIGSYVQDENESEAFDLFKRMNLCGIVSDGACLASTLKACTHVHGLYQGKQVHCLSVKCGLDRDLHTGSSLIDMYSKCGIIKDARKVFSSLPEWSVVSMNALIAGYS-QNNLEEAVVLFQEMLTRGVNPSEITFATIVEACHKPESLTLGTQFHGQITKRGFSSEGEYLGISLLGMYMNSRGMTEACALFSELSSPKSIVLWTGMMSGHSQNGFYEEALKFYKEMRHDGVLPDQATFVTVLRVCSVLSSLREGRAIHSLIFHLAHDLDELTSNTLIDMYAKCGDMKGSSQVFDEMRRRSNVVSWNSLINGYAKNGYAED-ALKIFDSMRQSHIMPDEITFLGVLTACSHAGKVSDGRKIFEMMIGQYGIEARVDHVACMVDLLGRWGYLQEADDFIEAQNLKPDARLWSSLLGA 911          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A0D2SEX4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G158100 PE=4 SV=1)

HSP 1 Score: 1853.95 bits (4801), Expect = 0.000e+0
Identity = 929/944 (98.41%), Postives = 933/944 (98.83%), Query Frame = -2

Query:  919 MLIQSLVFRCLSTRKNHLVIEFPRRLLXXXXXXXXXXXXXXATEEINPSLKVPLFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDYC 3750
            MLIQSLVFRCLSTRKN LVIEFPRRLL ASFQFVSSF+AHVATEEINPSLKVPLFKEKTKLEG+KRLKLYSKILHDCASKGSLSSAKAVHGKIIK G DPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSW ALFSGLVNEGYGY VLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLD+YSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMSSWNSASWNSLLSGS NGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGD+FVSSSIVDMYANCGCIEEAEAAF GMVSTDTVSWN MLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLG CRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDG HPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDYC
Sbjct:    1 MLIQSLVFRCLSTRKNRLVIEFPRRLLFASFQFVSSFTAHVATEEINPSLKVPLFKEKTKLEGSKRLKLYSKILHDCASKGSLSSAKAVHGKIIKNGIDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWAALFSGLVNEGYGYYVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDMYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMSSWNSASWNSLLSGSHNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDMFVSSSIVDMYANCGCIEEAEAAFQGMVSTDTVSWNTMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGACRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGFHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDYC 944          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A0B0NSU1_GOSAR (Pentatricopeptide repeat-containing-like protein OS=Gossypium arboreum GN=F383_23794 PE=4 SV=1)

HSP 1 Score: 1618.98 bits (4191), Expect = 0.000e+0
Identity = 798/810 (98.52%), Postives = 801/810 (98.89%), Query Frame = -2

Query:  940 MPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGI 3369
            MPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLD+FVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIK GCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACI KHGFESEIILSNALIAMYMKIRSVQNGW+VFKEMS WNSASWNSLLSGS NGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNM MLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAF GMVSTDTVSWNMMLFGYLQHRQGLKVLETFR MLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEV+SFIKDMKVTSNPLIWETVLG CRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDV DDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGI
Sbjct:    1 MPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDIFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKIGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACILKHGFESEIILSNALIAMYMKIRSVQNGWQVFKEMSFWNSASWNSLLSGSHNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMGMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFQGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRTMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVKSFIKDMKVTSNPLIWETVLGACRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVGDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGI 810          

HSP 2 Score: 207.994 bits (528), Expect = 4.078e-52
Identity = 150/574 (26.13%), Postives = 279/574 (48.61%), Query Frame = -2

Query: 1816 LHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMML 3531
            L  C+    L     +HG+ +K G   D+ + +SLV+ YAK G    A +V   M +++VVSW AL +G   +G    +L+L+  M +  +  +   L   LK+C+  ++L +G + H   +K+G   D FVG  L+DLY+KCG  E   +VF  + + N+V+W+A+++    +G   +   LF  M    +  ++ T +++      L +  +    H+ ++K G E +  +   L+ +Y K    ++  +VFK +   N  +W++++              +F  M  +G  P+  TF SI    +NL +  +   VHA I K+G +   ++  +LI +Y K   + +   +F ++   +  SW +L++G       ++    F+ M  +G  P+  T  + L SCSN+  ++ G  +H+  IK G   +  V +S++D+YA  G + +AE  F  ++  D  SW  ++ GY Q     K ++ F  M  +G+ P+  T    LS+CS M  +  G +   S+    G    +   + ++D+    G   E ++    M V+++ + W  +L
Sbjct:   48 LKACSLSLDLFFGTLLHGEGVKVGVLLDIFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKIGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACILKHGFESEIILSNALIAMYMKIRSVQNGWQVFKEMSFWNSASWNSLLSGSHNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMGMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFQGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRTMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVKSFIKDMKVTSNPLIWETVL 621          

HSP 3 Score: 157.918 bits (398), Expect = 4.410e-36
Identity = 109/412 (26.46%), Postives = 207/412 (50.24%), Query Frame = -2

Query: 2305 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCS 3540
            S +L  C    +L+     H  +IK G + D  +   L++ Y+K G+     KV  R+ E ++V+W+A+   L  +G   +  +L+  M+  GV PN H   +     +   D  +   +H   +K G   ++ + ++L+ +Y K   ++   +VF  M   N  SWN+LL+G        +  ++F  M     +    T +++L+SC+ L+N+ +G   H+ +IK G + +  VG  L+DLY+K G   D   +F ++ E ++ +W+A+I    +    ++A + F+ M+ +GV+PN  T A+     +N+G     + +H+   K G   ++ +S++++ MY     ++     F+ M S ++ SWN +L G    +   +    F  ML +G +PD  TF ++L +CS
Sbjct:  146 SNVLKSCTYLVNLTWGLIAHSLVIKIGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACILKHGFESEIILSNALIAMYMKIRSVQNGWQVFKEMSFWNSASWNSLLSGSHNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMGMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFQGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRTMLDKGLEPDEVTFIAVLSACS 557          

HSP 4 Score: 129.798 bits (325), Expect = 3.038e-27
Identity = 76/213 (35.68%), Postives = 109/213 (51.17%), Query Frame = -2

Query: 2905 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYL 3543
            ++ IL  C++  ++     VH  IIK G   +  +  SL++ YAK G    A  +  ++ ERD+ SWTAL +G          +  +  M++ GV PN   L T L +CS    L  G LLH   +K G   DVFV SS+VD+YA CG +E AE  F  M   + VSWN +L GY       K+L  F+ M +  L   + T   VL +C+Y+
Sbjct:  347 FTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMGMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFQGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRTMLDKGLEPDEVTFIAVLSACSYM 559          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A061F6E7_THECC (Pentatricopeptide repeat superfamily protein, putative OS=Theobroma cacao GN=TCM_030800 PE=4 SV=1)

HSP 1 Score: 1569.29 bits (4062), Expect = 0.000e+0
Identity = 782/946 (82.66%), Postives = 848/946 (89.64%), Query Frame = -2

Query:  925 MLIQSLVFRCLSTRKNHLVIEFPRRLLXXXXXXXXXXXXXXATEEINP----SLKVPLFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQD 3750
            ML+ SL FRC S RK+ L I+FPRR L ASFQ VS F+  VAT EINP     LKV  FKEK KL+  KRLKLYSKILHDCASKGSL  AK VHGKI++ G DPDLHLWNSLVN YAK G FGYACKVLD+MPE DVVSWTALFSGLVNEG+G  VL LYC MKKDGV PNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCG+MELAERVF++MDKKNVVSWNALLNGYAL+GDAGK+LNLF+GMTESELRCSKFTLSNVLKSCTYL NLTWGLIAHS+VIK+GCEHDEFVGCCLLD+YSKCGLAED LKVF+RIQEPNIVAWSAMIDCLDEQGQIQEAAE+F LMR KGVSPNQHTF+SIAG A NLGD+ +CEG+HACI K+GFESE +LSNALI+MYMKIRSVQNGW+VFKEMSSW+ ASWN+LLSG  + KTCD+GP IFHKMLA GF+PDICTF SILRSCS+LLN+KFG QVHAHIIKNGL  NNLVGTSLIDLYAKN FL DAELLF+QLIERDLFSWTALIAGYAQ++  EKAIKCFN +QRQGV PN F LATCLSSCS MAMLENGQLLHSMAIKAG+S D+FVSS++VDMYANCGCIEEAE+AF GM S D VSWN MLFGYLQH QGLKVLETFR MLDKGLEPDEVTFI VLSACSYMG V EGKE+FDSLTNV+GIVPTI+H ACMIDILGRAGKFNEVESF+KD KVTS  LIWETVLG+CRMHGNDK GE AAEKLFEL+P  ASHYILLANIFAAK RW+DVRRVRALMT  GVKKEPGCSWVMVNGQ+HIFRS D  HP + EIY+KL+ELV+K ILAGYVPKT+H+L++VS  EK+EQLF HNERLALAFALISINP+KT+RIFKNL ICEDCHDFMKLVSG+I+QEIVVRD NCFHHF+SGICSCQD
Sbjct:    1 MLVPSLDFRCFSLRKHSLFIKFPRRSLLASFQLVSCFTTLVATNEINPIFYPPLKVTPFKEKVKLDRTKRLKLYSKILHDCASKGSLRLAKVVHGKIMENGLDPDLHLWNSLVNVYAKCGSFGYACKVLDKMPETDVVSWTALFSGLVNEGHGSAVLGLYCFMKKDGVRPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGEMELAERVFVYMDKKNVVSWNALLNGYALEGDAGKVLNLFEGMTESELRCSKFTLSNVLKSCTYLGNLTWGLIAHSVVIKSGCEHDEFVGCCLLDMYSKCGLAEDALKVFQRIQEPNIVAWSAMIDCLDEQGQIQEAAEMFCLMRRKGVSPNQHTFSSIAGAATNLGDQFFCEGIHACIIKYGFESENVLSNALISMYMKIRSVQNGWQVFKEMSSWDLASWNALLSGPHDDKTCDQGPIIFHKMLAAGFRPDICTFASILRSCSSLLNLKFGQQVHAHIIKNGLNGNNLVGTSLIDLYAKNRFLEDAELLFSQLIERDLFSWTALIAGYAQTNRVEKAIKCFNQIQRQGVKPNEFILATCLSSCSKMAMLENGQLLHSMAIKAGHSADLFVSSALVDMYANCGCIEEAESAFQGMASADVVSWNTMLFGYLQHGQGLKVLETFRTMLDKGLEPDEVTFIGVLSACSYMGLVEEGKEHFDSLTNVYGIVPTIEHYACMIDILGRAGKFNEVESFVKDTKVTSTALIWETVLGSCRMHGNDKFGEIAAEKLFELDPETASHYILLANIFAAKCRWDDVRRVRALMTSHGVKKEPGCSWVMVNGQLHIFRSADSSHPMNREIYMKLQELVQKVILAGYVPKTEHVLHNVSHREKMEQLFCHNERLALAFALISINPMKTVRIFKNLLICEDCHDFMKLVSGIIEQEIVVRDVNCFHHFRSGICSCQD 946          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: F6HGR7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_11s0016g02500 PE=4 SV=1)

HSP 1 Score: 1112.83 bits (2877), Expect = 0.000e+0
Identity = 542/867 (62.51%), Postives = 672/867 (77.51%), Query Frame = -2

Query:  922 CASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3522
            CASKG L+  KA+HG++IK G +PD HLWNSLVN YAK G   YACKV   +PERDVVSWTAL +G V EGYG   ++L+C M+++GV  N     TALKACS+ LDL FG  +H E +KVG   D+FVGS+LVDLYAKCG+M LAERVFL M K+N VSWNALLNG+A  GDA K+LNLF  MT SE+  SKFTLS VLK C    NL  G I HSL I+ GCE DEF+ CCL+D+YSKCGLA D LKVF RI++P++V+WSA+I CLD++GQ +EAAE+F  MR  GV PNQ T AS+   A +LGD  Y E +HAC+ K+GFE +  + NAL+ MYMKI SVQ+G RVF+  ++ +  SWN+LLSG  + +TCD G  IF++MLAEGF P++ TF SILRSCS+L ++  G QVHA I+KN L  N+ VGT+L+D+YAKN FL DAE +F +LI+RDLF+WT ++AGYAQ    EKA+KCF  MQR+GV PN FTLA+ LS CS +A L++G+ LHSMAIKAG SGD+FV+S++VDMYA CGC+E+AE  F G+VS DTVSWN ++ GY QH QG K L+ F  MLD+G  PDEVTFI VLSACS+MG + EGK++F+SL+ ++GI PTI+H ACM+DILGRAGKF+EVESFI++MK+TSN LIWETVLG C+MHGN + GE AA KLFEL P I S+YILL+N+FAAKG W+DV  VRALM+  GVKKEPGCSWV VNGQVH+F S DG HPK  EI++KL++L +K +  GY P TDH+L++VSD EK E LF+H+ERLALAFAL+S +  KTIRIFKNL IC DCHDFMK +S +  QE+VVRD NCFHHFK+G CSCQ++
Sbjct:   43 CASKGDLNEGKAIHGQVIKSGINPDSHLWNSLVNVYAKCGSANYACKVFGEIPERDVVSWTALITGFVAEGYGSGAVNLFCEMRREGVEANEFTYATALKACSMCLDLEFGKQVHAEAIKVGDFSDLFVGSALVDLYAKCGEMVLAERVFLCMPKQNAVSWNALLNGFAQMGDAEKVLNLFCRMTGSEINFSKFTLSTVLKGCANSGNLRAGQIVHSLAIRIGCELDEFISCCLVDMYSKCGLAGDALKVFVRIEDPDVVSWSAIITCLDQKGQSREAAEVFKRMRHSGVIPNQFTLASLVSAATDLGDLYYGESIHACVCKYGFEYDNTVCNALVTMYMKIGSVQDGCRVFEATTNRDLISWNALLSGFHDNETCDTGLRIFNQMLAEGFNPNMYTFISILRSCSSLSDVDLGKQVHAQIVKNSLDGNDFVGTALVDMYAKNRFLEDAETIFNRLIKRDLFAWTVIVAGYAQDGQGEKAVKCFIQMQREGVKPNEFTLASSLSGCSRIATLDSGRQLHSMAIKAGQSGDMFVASALVDMYAKCGCVEDAEVVFDGLVSRDTVSWNTIICGYSQHGQGGKALKAFEAMLDEGTVPDEVTFIGVLSACSHMGLIEEGKKHFNSLSKIYGITPTIEHYACMVDILGRAGKFHEVESFIEEMKLTSNVLIWETVLGACKMHGNIEFGERAAMKLFELEPEIDSNYILLSNMFAAKGMWDDVTNVRALMSTRGVKKEPGCSWVEVNGQVHVFLSHDGSHPKIREIHLKLQDLHQKLMSVGYTPNTDHVLHNVSDREKQELLFYHSERLALAFALLSTSTRKTIRIFKNLRICGDCHDFMKSISEITNQELVVRDINCFHHFKNGSCSCQNF 909          

HSP 2 Score: 285.034 bits (728), Expect = 2.933e-77
Identity = 192/641 (29.95%), Postives = 331/641 (51.64%), Query Frame = -2

Query: 1627 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQL-LHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPT 3543
            Y+  L  C+    L   K VH + IK G   DL + ++LV+ YAK G    A +V   MP+++ VSW AL +G    G    VL+L+C M    +  +   L T LK C+ S +L  G ++H   +++G  LD F+   LVD+Y+KCG    A +VF+ ++  +VVSW+A++     KG + +   +F+ M  S +  ++FTL++++ + T L +L +G   H+ V K G E+D  V   L+ +Y K G  +D  +VF+     ++++W+A++    +         +F+ M  +G +PN +TF SI    ++L D    + VHA I K+  +    +  AL+ MY K R +++   +F  +   +  +W  +++G       ++    F +M  EG KP+  T  S L  CS +  +  G Q+H+  IK G   +  V ++L+D+YAK G + DAE++F  L+ RD  SW  +I GY+Q     KA+K F  M  +G  P+  T    LS+CS+M ++E G+   +S++   G +  +   + +VD+    G   E E+    M ++++ + W  +L G  +    ++  E   M L + LEP+  +   +LS       +   K  +D +TNV  ++ T
Sbjct:  137 YATALKACSMCLDLEFGKQVHAEAIKVGDFSDLFVGSALVDLYAKCGEMVLAERVFLCMPKQNAVSWNALLNGFAQMGDAEKVLNLFCRMTGSEINFSKFTLSTVLKGCANSGNLRAGQIVHSLAIRIGCELDEFISCCLVDMYSKCGLAGDALKVFVRIEDPDVVSWSAIITCLDQKGQSREAAEVFKRMRHSGVIPNQFTLASLVSAATDLGDLYYGESIHACVCKYGFEYDNTVCNALVTMYMKIGSVQDGCRVFEATTNRDLISWNALLSGFHDNETCDTGLRIFNQMLAEGFNPNMYTFISILRSCSSLSDVDLGKQVHAQIVKNSLDGNDFVGTALVDMYAKNRFLEDAETIFNRLIKRDLFAWTVIVAGYAQDGQGEKAVKCFIQMQREGVKPNEFTLASSLSGCSRIATLDSGRQLHSMAIKAGQSGDMFVASALVDMYAKCGCVEDAEVVFDGLVSRDTVSWNTIICGYSQHGQGGKALKAFEAMLDEGTVPDEVTFIGVLSACSHMGLIEEGKKHFNSLSKIYGITPTIEHYACMVDILGRAGKFHEVESFIEEMKLTSNVLIWETVL-GACKMHGNIEFGERAAMKLFE-LEPEIDSNYILLSN------MFAAKGMWDDVTNVRALMST 769          

HSP 3 Score: 196.052 bits (497), Expect = 5.969e-48
Identity = 129/417 (30.94%), Postives = 218/417 (52.28%), Query Frame = -2

Query: 1669 SCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKE 2919
            +C    +L  G   H  VIK+G   D  +   L+++Y+KCG A    KVF  I E ++V+W+A+I     +G    A  LF  MR +GV  N+ T+A+     +   D  + + VHA   K G  S++ + +AL+ +Y K   +    RVF  M   N+ SWN+LL+G       ++  ++F +M          T +++L+ C+N  N++ G  VH+  I+ G + +  +   L+D+Y+K G   DA  +F ++ + D+ SW+A+I    Q   S +A + F  M+  GV PN FTLA+ +S+ +++  L  G+ +H+   K G   D  V +++V MY   G +++    F    + D +SWN +L G+  +      L  F  ML +G  P+  TFI++L +CS +  V+ GK+
Sbjct:   42 TCASKGDLNEGKAIHGQVIKSGINPDSHLWNSLVNVYAKCGSANYACKVFGEIPERDVVSWTALITGFVAEGYGSGAVNLFCEMRREGVEANEFTYATALKACSMCLDLEFGKQVHAEAIKVGDFSDLFVGSALVDLYAKCGEMVLAERVFLCMPKQNAVSWNALLNGFAQMGDAEKVLNLFCRMTGSEINFSKFTLSTVLKGCANSGNLRAGQIVHSLAIRIGCELDEFISCCLVDMYSKCGLAGDALKVFVRIEDPDVVSWSAIITCLDQKGQSREAAEVFKRMRHSGVIPNQFTLASLVSAATDLGDLYYGESIHACVCKYGFEYDNTVCNALVTMYMKIGSVQDGCRVFEATTNRDLISWNALLSGFHDNETCDTGLRIFNQMLAEGFNPNMYTFISILRSCSSLSDVDLGKQ 458          

HSP 4 Score: 166.392 bits (420), Expect = 1.590e-38
Identity = 124/391 (31.71%), Postives = 195/391 (49.87%), Query Frame = -2

Query: 1258 KTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDIL---GRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGES--AAEKLFEL-NPGIASHYILLANIFAAKGRWEDV---RRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCH 2403
            +TCD+GP I  ++L EGF+P++        +C++  ++  G  +H  +IK+G+  ++ +  SL+++YAK G    A  +F ++ ERD+ SWTALI G+        A+  F  M+R+GV  N FT AT L +CS    LE G+ +H+ AIK G+  D+FV S++VD+YA CG +  AE  FL M   + VSWN +L G+ Q     KVL  F  M    +   + T   VL  C+  G +  G +   SL    G         C++D+    G AG   +V   I+D  V S    W  ++ TC     D+ G+S  AAE    + + G+  +   LA++ +A     D+     + A +   G + +      +V   + I    DGC 
Sbjct:   20 ETCDQGPRILIQLLVEGFEPNM--------TCASKGDLNEGKAIHGQVIKSGINPDSHLWNSLVNVYAKCGSANYACKVFGEIPERDVVSWTALITGFVAEGYGSGAVNLFCEMRREGVEANEFTYATALKACSMCLDLEFGKQVHAEAIKVGDFSDLFVGSALVDLYAKCGEMVLAERVFLCMPKQNAVSWNALLNGFAQMGDAEKVLNLFCRMTGSEINFSKFTLSTVLKGCANSGNLRAG-QIVHSLAIRIGCELDEFISCCLVDMYSKCGLAGDALKVFVRIEDPDVVS----WSAII-TCL----DQKGQSREAAEVFKRMRHSGVIPNQFTLASLVSAATDLGDLYYGESIHACVCKYGFEYDNTVCNALVTMYMKIGSVQDGCR 392          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: U5FHM8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0018s06910g PE=4 SV=1)

HSP 1 Score: 1067.37 bits (2759), Expect = 0.000e+0
Identity = 517/870 (59.43%), Postives = 660/870 (75.86%), Query Frame = -2

Query:  925 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQD 3534
            +L +CASKG +   KA+HG +I  G + D HLW SL+N YAK     +A KVL  MP+RDVVSWTAL SG VNEG G + + LYC M+K+ V  N   L TALKACS+ L+L FG  +H E +K G+LLD+FVG++LVDLYA+CG+MELAER+F  M  KN VSWNALLNGYA  GD  K+L LF  M E E + SKFTLS VLK C    +L  G + H+L +++GCE DEF+GC L+D+YSKCG   D LKVF +I+ P++VAWSAMI  LD+QG  QEAAELF LMR KG  PNQ T +S+   A N+GD  Y + +H CI K+GFES+ ++SN LI MYMK R V++G +VF+ M++ +  SWN+LLSG  + +TC RGP IF++ML EGFKP++ TF S+LRSCS+LL+ +FG QVHAHIIKN   D++ VGT+L+D+YAK   L DA + F +L+ RD+FSWT +I+GYAQ+D +EKA+K F  MQR+G+ PN +TLA+CLS CS+MA LENG+ LH++A+KAG+ GD+FV S++VD+Y  CGC+E AEA F G++S D VSWN ++ GY QH QG K LE FRMML +G+ PDE TFI VLSACS+MG V EGK+ FDS++ ++GI P+I+H ACM+DILGRAGKFNEV+ FI++M +T   LIWETVLG C++HGN   GE AA+KLFE+ P + S YILL+NIFA+KGRW+DVR +RALMT  G+KKEPGCSWV V+GQVH+F S DG HPK  EIY KL +L +  +  GYVPKT+ +L++VS+ EK+E L++H+ERLAL+FAL+S N VK IRIFKNL ICEDCHDFMKL+S +  QEIVVRD   FHHFK G CSCQD
Sbjct:    1 MLRECASKGDVKEGKAIHGNLITSGVELDSHLWVSLINFYAKCRSRFFARKVLAEMPQRDVVSWTALISGFVNEGCGSESVSLYCEMRKENVRANEFALATALKACSMCLNLEFGKQVHVEAIKAGLLLDLFVGTALVDLYARCGEMELAERLFFGMPDKNGVSWNALLNGYAQLGDGKKVLKLFCKMKECETKFSKFTLSTVLKGCANTGSLREGKVLHALALRSGCEIDEFLGCSLVDMYSKCGTVYDALKVFTKIRNPDVVAWSAMITGLDQQGHGQEAAELFHLMRRKGARPNQFTLSSLVSTATNMGDLRYGQSIHGCICKYGFESDNLVSNPLIMMYMKSRCVEDGNKVFEAMTNPDLVSWNALLSGFYDSQTCGRGPRIFYQMLLEGFKPNMFTFISVLRSCSSLLDPEFGKQVHAHIIKNSSDDDDFVGTALVDMYAKARCLEDAGVAFDRLVNRDIFSWTVIISGYAQTDQAEKAVKYFRQMQREGIKPNEYTLASCLSGCSHMATLENGRQLHAVAVKAGHFGDIFVGSALVDLYGKCGCMEHAEAIFKGLISRDIVSWNTIISGYSQHGQGEKALEAFRMMLSEGIMPDEATFIGVLSACSFMGLVEEGKKRFDSMSKIYGINPSIEHYACMVDILGRAGKFNEVKIFIEEMNLTPYSLIWETVLGACKLHGNVDFGEKAAKKLFEMEPMMDSSYILLSNIFASKGRWDDVRNIRALMTSRGIKKEPGCSWVEVDGQVHVFLSQDGSHPKIREIYAKLDKLGQSLMSIGYVPKTEVVLHNVSNKEKMEHLYYHSERLALSFALLSTNAVKPIRIFKNLRICEDCHDFMKLISDITNQEIVVRDIRRFHHFKRGTCSCQD 870          

HSP 2 Score: 204.912 bits (520), Expect = 6.243e-51
Identity = 128/412 (31.07%), Postives = 217/412 (52.67%), Query Frame = -2

Query: 2305 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCS 3540
            S +L  CA+ GSL   K +H   ++ G + D  L  SLV+ Y+K G    A KV  ++   DVV+W+A+ +GL  +G+G +  +L+  M++ G  PN   L + +   +   DL +G  +HG   K G   D  V + L+ +Y K   +E   +VF  M   ++VSWNALL+G+      G+   +F  M     + + FT  +VL+SC+ L++  +G   H+ +IK   + D+FVG  L+D+Y+K    ED    F R+   +I +W+ +I    +  Q ++A + F  M+ +G+ PN++T AS     +++        +HA   K G   +I + +AL+ +Y K   +++   +FK + S +  SWN+++SG       ++    F  ML+EG  PD  TF  +L +CS
Sbjct:  201 STVLKGCANTGSLREGKVLHALALRSGCEIDEFLGCSLVDMYSKCGTVYDALKVFTKIRNPDVVAWSAMITGLDQQGHGQEAAELFHLMRRKGARPNQFTLSSLVSTATNMGDLRYGQSIHGCICKYGFESDNLVSNPLIMMYMKSRCVEDGNKVFEAMTNPDLVSWNALLSGFYDSQTCGRGPRIFYQMLLEGFKPNMFTFISVLRSCSSLLDPEFGKQVHAHIIKNSSDDDDFVGTALVDMYAKARCLEDAGVAFDRLVNRDIFSWTVIISGYAQTDQAEKAVKYFRQMQREGIKPNEYTLASCLSGCSHMATLENGRQLHAVAVKAGHFGDIFVGSALVDLYGKCGCMEHAEAIFKGLISRDIVSWNTIISGYSQHGQGEKALEAFRMMLSEGIMPDEATFIGVLSACS 612          

HSP 3 Score: 137.887 bits (346), Expect = 1.117e-29
Identity = 91/285 (31.93%), Postives = 140/285 (49.12%), Query Frame = -2

Query: 2734 LEGAK-RLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWG-----LIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWS 3570
            LEG K  +  +  +L  C+S       K VH  IIK   D D  +  +LV+ YAK      A    DR+  RD+ SWT + SG          +  +  M+++G+ PN + L + L  CS    L  G  LH   VK G   D+FVGS+LVDLY KCG ME AE +F  +  +++VSWN +++GY+  G   K L  F+ M    +   + T   VL +C+++  +  G      ++    I    EH      C++D+  + G   + +K+F  I+E N+  +S
Sbjct:  392 LEGFKPNMFTFISVLRSCSSLLDPEFGKQVHAHIIKNSSDDDDFVGTALVDMYAKARCLEDAGVAFDRLVNRDIFSWTVIISGYAQTDQAEKAVKYFRQMQREGIKPNEYTLASCLSGCSHMATLENGRQLHAVAVKAGHFGDIFVGSALVDLYGKCGCMEHAEAIFKGLISRDIVSWNTIISGYSQHGQGEKALEAFRMMLSEGIMPDEATFIGVLSACSFMGLVEEGKKRFDSMSKIYGINPSIEHY----ACMVDILGRAGKFNE-VKIF--IEEMNLTPYS 669          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: M5VT93_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020478mg PE=4 SV=1)

HSP 1 Score: 1060.83 bits (2742), Expect = 0.000e+0
Identity = 525/871 (60.28%), Postives = 656/871 (75.32%), Query Frame = -2

Query:  922 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3534
            +L  C  +GSL+  KA+HG++IK G DPDLHLW SLVN YAK G  GYA KVLD MPE+DVVSWT L  G V  G+G D + L+C MKKDG   N   L T LKACSL  DL FG  LH E VK+G   DVFVGS+LV LYAKCG+MELA+ V   M ++NVVSWNALLNGYA +GD  ++L LF  MTESE+R SKFTLS VLK C    NL  G   HSL IK+GC+ DEF+GC L+D+YSKCG+A D +KVF+RI+ P++VAWSA+I CLD+QGQ QE AELF  M   G+SPNQ + +SI   A +L D  + E VHA  +K+G ES+I +SNALI MYMKI  V +G +VF+ M+  +  SWNSLLSG  N + CD GP IF +ML EGFKP++ +F S+LRSCS+LL++  G QVHAHI+K  L DN+ VGT+LID+YAK  FL DA + F +L  RDLF WT +I GYAQ+D +EKA+ CF+ MQ++GV PN F LA CLS+CS +AMLENG+ LHSMAIK+G+ GD+FVSS++VDMYA CGCI +AE  F G+ S DTVSWN+M+ GY Q+ +G K +E F  ML++G  PDEVTFI +LSACS++G V EGK++FDSL+ VF I PTI+H ACM+DIL RAGKFNE ESFI+ MK+T  P+IWETVLG C+M+GN + GE+AA+KLFEL P + S YILL+NIFA KGRW+DV +VR LM+  GVKK+PGCSWV V+GQV+ F S DG HP+  +I++KL+EL EK    GY+P+T+ +L+++++ EK E L +H+ERLALAF+LIS NP KTIRIFKNL IC DCH+ MKL+S V  +EIVVRD   FHHFKSG CSC D+
Sbjct:    1 MLRTCVLQGSLNEGKAIHGQVIKNGIDPDLHLWVSLVNVYAKCGDCGYARKVLDEMPEQDVVSWTTLIQGFVVNGFGVDAVKLFCEMKKDGTRANEFALATGLKACSLCFDLGFGKQLHAEAVKLGFFSDVFVGSALVGLYAKCGEMELADTVLFCMPEQNVVSWNALLNGYAQEGDGKQVLKLFCRMTESEMRLSKFTLSTVLKGCANSENLRGGQFLHSLAIKSGCKIDEFLGCSLVDMYSKCGMAIDAVKVFRRIKNPDVVAWSAIITCLDQQGQCQEVAELFREMISTGISPNQFSLSSIISAATDLKDLHFGESVHAFAWKYGCESDISVSNALITMYMKIGRVLDGAQVFEAMTDRDLISWNSLLSGMHNHEICDLGPRIFRQMLVEGFKPNMYSFISVLRSCSSLLDVGLGKQVHAHIVKTSLDDNDFVGTALIDMYAKIRFLEDAVIAFNKLSNRDLFIWTVIITGYAQTDQAEKAVACFSQMQQEGVKPNEFALAGCLSACSRIAMLENGRQLHSMAIKSGHLGDLFVSSALVDMYAKCGCIGDAEDIFGGLDSCDTVSWNIMICGYSQYGRGEKAIEAFSTMLNEGTIPDEVTFIGILSACSHLGLVEEGKKHFDSLSKVFRITPTIEHYACMVDILVRAGKFNEAESFIETMKLTLYPIIWETVLGACKMYGNVEFGETAAKKLFELKPEMDSTYILLSNIFAVKGRWDDVSKVRKLMSSQGVKKKPGCSWVEVDGQVNTFVSQDGSHPRIRDIHLKLEELGEKLNSVGYIPETEDVLHNITEREKNEHLQYHSERLALAFSLISTNPPKTIRIFKNLRICGDCHEVMKLISDVTNREIVVRDIKRFHHFKSGTCSCNDF 871          

HSP 2 Score: 181.415 bits (459), Expect = 1.914e-43
Identity = 120/414 (28.99%), Postives = 211/414 (50.97%), Query Frame = -2

Query: 2299 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNL 3540
            S +L  CA+  +L   + +H   IK G   D  L  SLV+ Y+K G+   A KV  R+   DVV+W+A+ + L  +G   +V +L+  M   G+ PN   L + + A +   DL FG  +H    K G   D+ V ++L+ +Y K G++    +VF  M  ++++SWN+LL+G            +F+ M     + + ++  +VL+SC+ L+++  G   H+ ++KT  + ++FVG  L+D+Y+K    ED +  F ++   ++  W+ +I    +  Q ++A   FS M+ +GV PN+   A      + +        +H+   K G   ++ +S+AL+ MY K   + +   +F  + S ++ SWN ++ G       ++    F  ML EG  PD  TF  IL +CS+L
Sbjct:  201 STVLKGCANSENLRGGQFLHSLAIKSGCKIDEFLGCSLVDMYSKCGMAIDAVKVFRRIKNPDVVAWSAIITCLDQQGQCQEVAELFREMISTGISPNQFSLSSIISAATDLKDLHFGESVHAFAWKYGCESDISVSNALITMYMKIGRVLDGAQVFEAMTDRDLISWNSLLSGMHNHEICDLGPRIFRQMLVEGFKPNMYSFISVLRSCSSLLDVGLGKQVHAHIVKTSLDDNDFVGTALIDMYAKIRFLEDAVIAFNKLSNRDLFIWTVIITGYAQTDQAEKAVACFSQMQQEGVKPNEFALAGCLSACSRIAMLENGRQLHSMAIKSGHLGDLFVSSALVDMYAKCGCIGDAEDIFGGLDSCDTVSWNIMICGYSQYGRGEKAIEAFSTMLNEGTIPDEVTFIGILSACSHL 614          

HSP 3 Score: 117.472 bits (293), Expect = 2.432e-23
Identity = 89/312 (28.53%), Postives = 138/312 (44.23%), Query Frame = -2

Query: 2665 PLFKEKTKLEGAKRLKLYS--KILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMR 3594
            P    +  +EG K   +YS   +L  C+S   +   K VH  I+K   D +  +  +L++ YAK      A    +++  RD+  WT + +G          +  +  M+++GV PN   L   L ACS    L  G  LH   +K G L D+FV S+LVD+YAKCG +  AE +F  +D  + VSWN ++ GY+  G   K +  F  M        + T   +L +C++L     GL      ++ G +H                   D+L    RI  P I  ++ M+D L   G+  EA      M+
Sbjct:  384 PRIFRQMLVEGFKP-NMYSFISVLRSCSSLLDVGLGKQVHAHIVKTSLDDNDFVGTALIDMYAKIRFLEDAVIAFNKLSNRDLFIWTVIITGYAQTDQAEKAVACFSQMQQEGVKPNEFALAGCLSACSRIAMLENGRQLHSMAIKSGHLGDLFVSSALVDMYAKCGCIGDAEDIFGGLDSCDTVSWNIMICGYSQYGRGEKAIEAFSTMLNEGTIPDEVTFIGILSACSHL-----GL------VEEGKKH------------------FDSLSKVFRIT-PTIEHYACMVDILVRAGKFNEAESFIETMK 664          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A0A0K4Y9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G388380 PE=4 SV=1)

HSP 1 Score: 984.171 bits (2543), Expect = 0.000e+0
Identity = 484/879 (55.06%), Postives = 631/879 (71.79%), Query Frame = -2

Query:  922 KRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3558
            K+LK YS +L +CASK SL  AKA+HG I+K   +PD HLW SLVN YAK     YA  VL +MP+RDVVSWTAL  GLV EG+  D + L+  M+ +G++PN   L T LKACSL + L  G  +H +  K+G+LLD+FVGS+LVDLYAKCG++ELA ++F+ M ++N V+WN LLNGYA +GD   +L LF  M E +++C++FTL+ VLK C    NL  G + HSL+IK G E +EF+GC L+D+YSKCGLA D + VFK I++P+IV WSA+I CLD+QGQ +E+ +LF LMR     PNQ+T  S+   A N G+  Y + +HAC++K+GFE+++ +SNAL+ MYMK   V +G ++++ M   +  SWN+ LSG  +    DR  +IF+ ML EGF P++ TF SIL SCS L ++ +G QVHAHIIKN L DNN V T+LID+YAK  +L DA++ F +L  RDLF+WT +I  YAQ++  EKA+  F  MQ++GV PN FTLA CLS CS++A LE GQ LHSM  K+G+  D+FV S++VDMYA CGC+EEAEA F  ++  DT++WN ++ GY Q+ QG K L  FRMMLD+G+ PD VTF  +LSACS+ G V EGKE+F+S+   FGI PT+ HCACM+DILGR GKF+E+E FI+ M+++ N LIWETVLG  +MH N  LGE AA KLFEL P   S YILL+NIFA +GRW+DV+RVR+LM+  GVKKEPGCSWV  NGQVH F S D  HP+  EI++KL EL  +     YVPKT+++L++V + EK E L  H+ERLAL FALIS +  K IRIFKNL IC DCHD MK +S +  QEIVVRD   FHHFK+G CSC D+
Sbjct:  215 KKLKYYSSMLRECASKRSLGVAKAIHGLIVKDVINPDSHLWVSLVNVYAKCRYSAYARLVLAKMPDRDVVSWTALIQGLVAEGFANDSIYLFQEMQNEGIMPNEFTLATGLKACSLCMALDLGKQMHAQAFKLGLLLDLFVGSALVDLYAKCGEIELASKMFIGMPEQNDVTWNVLLNGYAQRGDVTGVLKLFCSMMELDVKCNEFTLTTVLKGCANSKNLKQGQVIHSLIIKCGYEGNEFIGCGLVDMYSKCGLAIDAIGVFKTIKKPDIVVWSALITCLDQQGQSEESIKLFHLMRLGDTLPNQYTICSLLSAATNTGNLQYGQSIHACVWKYGFETDVAVSNALVTMYMKNGCVHDGTKLYESMVDRDLISWNAYLSGLHDCGMYDRPLTIFYHMLEEGFIPNMYTFISILGSCSCLFDVHYGRQVHAHIIKNQLDDNNFVCTALIDMYAKCMYLEDADVAFNRLSVRDLFTWTVIITNYAQTNQGEKALNYFRQMQQEGVKPNEFTLAGCLSGCSSLASLEGGQQLHSMVFKSGHVSDMFVGSALVDMYAKCGCMEEAEALFEALIRRDTIAWNTIICGYAQNGQGNKALTAFRMMLDEGISPDGVTFTGILSACSHQGLVEEGKEHFNSMYRDFGISPTVDHCACMVDILGRVGKFDELEDFIQKMQLSQNALIWETVLGASKMHNNLVLGEKAANKLFELQPEEESSYILLSNIFATEGRWDDVKRVRSLMSSKGVKKEPGCSWVEANGQVHTFVSHDYSHPQIQEIHLKLDELDRELASIQYVPKTEYVLHNVGETEKKENLRFHSERLALGFALISTSSEKKIRIFKNLRICRDCHDVMKHISSITNQEIVVRDVRRFHHFKNGACSCNDF 1093          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: M1BCU9_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG402016414 PE=4 SV=1)

HSP 1 Score: 966.066 bits (2496), Expect = 0.000e+0
Identity = 473/882 (53.63%), Postives = 630/882 (71.43%), Query Frame = -2

Query:  922 EGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3567
            E  + +K YS++L D A+K  L   KA+HG++I+ G +PD HLW SL+N Y+K G   +A  V D +P RDVVSWTAL +G + +GYG   + L+C M+ + + PN   L T LK CS+ LDL FG  LH   VK  V  DV+VGS+LVDLYAKC ++E A +VF  M ++N VSWN LLNGY   G   + L LF  M++SE+R S +TLS +LK C   VNL  G + HS+++K G E D+F  C LLD+Y+KCGL +D LKVF R +  +IVAW+AMI  LD+QGQ +EA +LF LM   G+ PNQ T AS+   AA+  D   C+ +HAC++K GF+SE  +SNALIAMYMK  SV +G+R+F  +S+ +  SWNSLLSG  + +T   GP IF ++L EG +P+I T  S LRSC++LL+   G QVHAH++K  L  N  VGT+L+D+YAK G L DAEL+F +L E+D+F+WT +I+GYAQSD  EKA +CFN MQR+ + PN FTLA+CL  CS +A L+NGQ LHS+ +K+G   D++V+S+++DMYA  GCI++AE+ F  M S+DTV WN +++ Y QH    K L+TFR ML +G+ PD +TFIAVLSACS++G V EG+E+FDS+ N FGI P+I+H ACM+DILGRAGKF E+E FI+ M++  + LIWETVLG C+ HGN +L E AA  LFE++P   S YILL+NI+A+KGRW DV  VRALM+  GVKKEPGCSW+ ++ QVH+F S D  HP+  +I+ KL+EL  +    GY+P T+++L++VSD EK++ L HH+ERLALAFAL+S +   TIRIFKNL IC DCH+FMKL S V  +EIV+RD N FHHF  G CSC+DY
Sbjct:  108 EKKRGIKWYSEMLKDYAAKLCLKEGKALHGEMIRSGVEPDSHLWVSLINFYSKCGDLVFAENVFDLIPSRDVVSWTALIAGFIAQGYGSKGICLFCDMRGEDIRPNEFTLATVLKGCSMCLDLEFGKQLHAVVVKGAVFSDVYVGSALVDLYAKCCELESAVKVFFSMPEQNSVSWNVLLNGYVQAGQGEEALKLFMKMSDSEMRFSNYTLSTILKGCANSVNLKAGQVIHSMLVKIGSEIDDFTSCSLLDMYNKCGLQDDALKVFLRTKNHDIVAWTAMISGLDQQGQKREAIQLFCLMMHSGLRPNQFTLASVVSAAADSVDLRCCKSIHACVYKFGFDSEECVSNALIAMYMKFGSVLDGYRIFSSLSNRDIISWNSLLSGFHDNETSYEGPKIFRQLLVEGLRPNIYTLISNLRSCASLLDASLGKQVHAHVVKADLGGNIYVGTALVDMYAKCGQLDDAELIFYRLSEKDVFTWTVVISGYAQSDQGEKAFRCFNQMQREAIKPNEFTLASCLKGCSRIASLDNGQQLHSVVMKSGQFSDMYVASALIDMYAKSGCIKDAESLFQSMESSDTVLWNTIIYAYSQHGLDEKALKTFRTMLSEGILPDGITFIAVLSACSHLGLVKEGQEHFDSIKNGFGITPSIEHYACMVDILGRAGKFTEMEHFIEGMELAPDALIWETVLGVCKAHGNVELAEKAANTLFEIDPKAESSYILLSNIYASKGRWADVSTVRALMSRQGVKKEPGCSWIEIDNQVHVFLSQDASHPRLKDIHKKLEELASRITATGYIPNTNYVLHNVSDKEKIDNLSHHSERLALAFALVSSSRNSTIRIFKNLCICGDCHEFMKLASIVTNREIVIRDINRFHHFSHGTCSCKDY 989          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: K4ASY3_SOLLC (Uncharacterized protein OS=Solanum lycopersicum GN=Solyc01g007850.2 PE=4 SV=1)

HSP 1 Score: 954.896 bits (2467), Expect = 0.000e+0
Identity = 469/882 (53.17%), Postives = 624/882 (70.75%), Query Frame = -2

Query:  922 EGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3567
            E  + +K YS++  D A K  L   KA+HG++I+ G +PD +LW SL+N Y+K G   +A  V D +P RDVVSWTAL +G + +GYG   + L+C MK + + PN   L T LK CS+ LDL FG  LH   VK     DV+VGS+LVDLYAKC ++E A +VF  M ++N VSWN LLNGY   G   + L LF  M++SE+R S +TLS +LK C   VNL  G + HS+++K G E D+F  C LLD+Y+KCGL +D LKVF R +  +IVAW+AMI  LD+QGQ +EA  LF LM   G+ PNQ T AS+   AA+  D   C+ +HAC++K GF+SE  + NALIAMYMK  SV +G+R+F  +S+ +  SWNSLLSG  + +T   GP IF ++L EG KP+I T  S LRSC++LL+   G QVHAH++K  L  N  VGT+L+D+YAK G L DAEL+F +L E+D+F+WT +I+GYAQSD  EKA +CFN MQR+ + PN FTLA+CL  CS +A L+NG+ LHS+ +K+G   D++V+S+++DMYA  GCI++AE+ F  M S+DTV WN +++ Y QH    + L+TFR ML +G+ PD +TFIAVLSACS++G V EG+ +FDS+ N FGI P+I+H ACM+DILGRAGKF E+E FI+ M +  + LIWETVLG C+ HGN +L E AA  LFE++P   S YILL+NI+A+KGRW DV  VRALM+  GVKKEPGCSW+ ++ QVH+F S D  HP+  +I+ KL+EL  +   AGY+P T+++L++VSD EK++ L HH+ERLALAFAL+S +   TIRIFKNL IC DCH+FMKL S V  +EIV+RD N FHHF  G CSC+DY
Sbjct:  136 EKKRGIKWYSEMFKDYAGKLCLKEGKALHGEMIRSGVEPDSYLWVSLINFYSKCGDLVFAENVFDLIPSRDVVSWTALIAGFIAQGYGSKGICLFCDMKGEDIRPNEFTLATVLKGCSMCLDLEFGKQLHAVVVKGAAFSDVYVGSALVDLYAKCCELESAVKVFFSMPEQNSVSWNVLLNGYVQAGQGEEALKLFLKMSDSEMRFSNYTLSTILKGCANSVNLKAGQVIHSMLVKIGSEIDDFTSCSLLDMYNKCGLQDDALKVFLRTKNHDIVAWTAMISGLDQQGQKREAIHLFCLMMHSGLRPNQFTLASVVSAAADSVDIRCCKSIHACVYKFGFDSEECVCNALIAMYMKFGSVLDGYRIFSSLSNRDIISWNSLLSGFHDNETSYEGPKIFRQLLVEGLKPNIYTLISNLRSCASLLDASLGKQVHAHVVKADLGGNIYVGTALVDMYAKCGQLDDAELIFYRLSEKDVFTWTVVISGYAQSDQGEKAFRCFNQMQREAIKPNEFTLASCLKGCSRIASLDNGRQLHSVVMKSGQFSDMYVASALIDMYAKSGCIKDAESLFQSMESSDTVLWNTIIYAYSQHGLDEEALKTFRTMLSEGIPPDGITFIAVLSACSHLGLVKEGRRHFDSIKNGFGITPSIEHYACMVDILGRAGKFTEMEHFIEGMALAPDALIWETVLGVCKAHGNVELAEKAANTLFEIDPKAESSYILLSNIYASKGRWADVSTVRALMSRQGVKKEPGCSWIEIDNQVHVFLSQDASHPRLKDIHKKLEELTSRITAAGYIPNTNYVLHNVSDKEKIDNLSHHSERLALAFALMSSSRNSTIRIFKNLCICGDCHEFMKLASIVTNREIVIRDINRFHHFSHGTCSCKDY 1017          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A068V2Q8_COFCA (Coffea canephora DH200=94 genomic scaffold, scaffold_80 OS=Coffea canephora GN=GSCOC_T00040403001 PE=4 SV=1)

HSP 1 Score: 952.97 bits (2462), Expect = 0.000e+0
Identity = 474/884 (53.62%), Postives = 629/884 (71.15%), Query Frame = -2

Query:  922 LEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCS-EKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3570
            L+  K L+ YS+    CA    L+  K  H  +IK G DPD HL+ SL+N YAK G   +A +VLD MPE+DVVSWTAL SG V EG G + ++L+C M+++G+ PN   L T L+ACS+   L FG  LH E VK     DV+VGS+LVDLYAKCG+ME A++VF  M ++N VSWN LLNGYA  GD  K+L LF  MTES++R S +TLS VLK C    +L  G + HS+ IK G   D+F+ C L+D+YSKC LA D L+VFK I++P+IV WS MI  LD+QGQ  +A ELF LM   G+ PNQ + +++   A +LGD  +C+ +HACI+K  FES++ +SNALI MYMK+  V +G +VF  MS  +  SWN+LLSG  +G++ D+GP IF KML EG  P+  TF S LRSC++ LN  FG QVHA+++KN L  +  VG +LID+Y+K   L D EL+F +L ERD+F+WT LIAGYAQ+D   EKA+  FN M R+GV  N FTLA+CLS+C+ +A L NGQ LHS AIK+G+  DV+V+S++VDMY  CGCI++AE  F  + + DTV WN M+ G+ +H Q  K L++F  ML+K ++P+ V+FI VLSACS+MG V EGK++F  ++ ++GI P++ H ACM+DILGRAG+F+E+ESFI+ MK+  N LIWETVLG C++HGN ++GE AA+KLFE++P   S YI L+NI+AAKGRW DV R+RALM+  GVKKEPGCSWV V+ + H+F S D  HP+  +IY KL++L ++    GY P T ++L++V D  K E LFHH+ERLALAFAL+S      IRIFKNL IC DCH+FMK VS +  +EIV+RD+N FHHF +GICSC+DY
Sbjct:  124 LDRGKVLRWYSETFRYCAVNLCLNEGKVFHANLIKIGIDPDSHLFVSLINFYAKCGALSFARRVLDEMPEKDVVSWTALISGFVAEGLGQEGVELFCEMRREGIRPNEFTLATVLRACSMVSGLEFGKQLHAEVVKGEAFTDVYVGSALVDLYAKCGEMEYADKVFFIMPEQNAVSWNVLLNGYAQLGDGHKLLILFCKMTESDMRFSNYTLSTVLKGCASSRSLWAGQVVHSMAIKIGSAFDDFISCGLVDMYSKCELANDALQVFKMIRDPDIVTWSTMISGLDQQGQKLQAVELFRLMMQSGLRPNQFSLSTVVSTATDLGDPHFCKSIHACIWKFHFESDLSVSNALITMYMKLGLVYDGLKVFSAMSQKDVVSWNALLSGYHDGESSDQGPMIFKKMLTEGLMPNQYTFISTLRSCTSQLNASFGKQVHAYLLKNNLCTDVHVGIALIDMYSKCRCLDDVELIFNRLSERDIFTWTVLIAGYAQTDNQGEKALGFFNRMHREGVKANEFTLASCLSACAGIASLINGQQLHSWAIKSGHFCDVYVASALVDMYGKCGCIDDAEMIFKSIETVDTVLWNTMICGHSKHGQNEKALQSFGAMLNKDVQPNGVSFIGVLSACSHMGLVEEGKKHFHLMSELYGIAPSVDHYACMVDILGRAGRFSELESFIQHMKIAPNTLIWETVLGACKIHGNVEMGEKAAQKLFEIDPDEDSSYIWLSNIYAAKGRWNDVSRIRALMSSRGVKKEPGCSWVEVDAKTHVFLSQDASHPRLTDIYQKLEDLHQRLQSIGYTPNTHYVLHNVPDAVKKENLFHHSERLALAFALVSNANGGRIRIFKNLRICGDCHEFMKGVSDITNKEIVIRDSNRFHHFHNGICSCKDY 1007