g.barbadense_cottongen_reftransV1_0091917, g.barbadense_cottongen_reftransV1_0091917 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP307_ARATH (Pentatricopeptide repeat-containing protein At4g13650 OS=Arabidopsis thaliana GN=PCMP-H42 PE=2 SV=2)

HSP 1 Score: 523.857 bits (1348), Expect = 1.215e-163
Identity = 279/875 (31.89%), Postives = 474/875 (54.17%), Query Frame = -2

Query:  922 YSKILHDCASKG-SLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3543
            +S +L  C     +    + +H +I+ +G      + N L++ Y++ G    A +V D +  +D  SW A+ SGL       + + L+C M   G++P  +   + L AC     L  G  LHG  +K+G   D +V ++LV LY   G +  AE +F  M +++ V++N L+NG +  G   K + LF+ M    L     TL++++ +C+    L  G   H+   K G   +  +   LL+LY+KC   E  L  F   +  N+V W+ M+        ++ +  +F  M+ + + PNQ+T+ SI      LGD    E +H+ I K  F+    + + LI MY K+  +   W +    +  +  SW ++++G       D+  + F +ML  G + D    T+ + +C+ L  +K G Q+HA    +G   +     +L+ LY++ G + ++ L F Q    D  +W AL++G+ QS  +E+A++ F  M R+G+  NNFT  + + + S  A ++ G+ +H++  K G   +  V ++++ MYA CG I +AE  FL + + + VSWN ++  Y +H  G + L++F  M+   + P+ VT + VLSACS++G V++G  YF+S+ + +G+ P  +H  C++D+L RAG  +  + FI++M +  + L+W T+L  C +H N ++GE AA  L EL P  ++ Y+LL+N++A   +W+     R  M   GVKKEPG SW+ V   +H F   D  HP   EI+   ++L +++   GYV     +LN++  ++K   +F H+E+LA++F L+S+     I + KNL +C DCH ++K VS V  +EI+VRDA  FHHF+ G CSC+DY
Sbjct:  189 FSGVLEACRGGSVAFDVVEQIHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDGLRLKDHSSWVAMISGLSKNECEAEAIRLFCDMYVLGIMPTPYAFSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTAWDILIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAVSACAGLQALKEGQQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEESYLAFEQTEAGDNIAWNALVSGFQQSGNNEEALRVFVRMNREGIDNNNFTFGSAVKAASETANMKQGKQVHAVITKTGYDSETEVCNALISMYAKCGSISDAEKQFLEVSTKNEVSWNAIINAYSKHGFGSEALDSFDQMIHSNVRPNHVTLVGVLSACSHIGLVDKGIAYFESMNSEYGLSPKPEHYVCVVDMLTRAGLLSRAKEFIQEMPIKPDALVWRTLLSACVVHKNMEIGEFAAHHLLELEPEDSATYVLLSNLYAVSKKWDARDLTRQKMKEKGVKKEPGQSWIEVKNSIHSFYVGDQNHPLADEIHEYFQDLTKRASEIGYVQDCFSLLNELQHEQKDPIIFIHSEKLAISFGLLSLPATVPINVMKNLRVCNDCHAWIKFVSKVSNREIIVRDAYRFHHFEGGACSCKDY 1063          

HSP 2 Score: 286.574 bits (732), Expect = 5.759e-79
Identity = 195/713 (27.35%), Postives = 342/713 (47.97%), Query Frame = -2

Query: 1390 SKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKAC---SLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFEL-NPGIASHYILLANIFAA 3516
            + GSL   + +H +I+K G D +  L   L + Y  +G    A KV D MPER + +W  +   L +     +V  L+  M  + V PN       L+AC   S++ D+     +H   +  G+     V + L+DLY++ G ++LA RVF  +  K+  SW A+++G +      + + LF  M    +  + +  S+VL +C  + +L  G   H LV+K G   D +V   L+ LY   G       +F  + + + V ++ +I+ L + G  ++A ELF  M   G+ P+ +T AS+    +  G     + +HA   K GF S   +  AL+ +Y K   ++     F E    N   WN +L              IF +M  E   P+  T+ SIL++C  L +++ G Q+H+ IIK   + N  V + LID+YAK G L  A  +  +   +D+ SWT +IAGY Q +  +KA+  F  M  +G+  +   L   +S+C+ +  L+ GQ +H+ A  +G S D+   +++V +Y+ CG IEE+  AF    + D ++WN ++ G+ Q     + L  F  M  +G++ +  TF + + A S    + +GK+    +T   G     + C  +I +  + G  ++ E    ++  T N + W  ++     HG    G  A +   ++ +  +  +++ L  + +A
Sbjct:   97 TNGSLDEGRKLHSQILKLGLDSNGCLSEKLFDFYLFKGDLYGAFKVFDEMPERTIFTWNKMIKELASRNLIGEVFGLFVRMVSENVTPNEGTFSGVLEACRGGSVAFDVV--EQIHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDGLRLKDHSSWVAMISGLSKNECEAEAIRLFCDMYVLGIMPTPYAFSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTAWDILIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAVSACAGLQALKEGQQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEESYLAFEQTEAGDNIAWNALVSGFQQSGNNEEALRVFVRMNREGIDNNNFTFGSAVKAASETANMKQGKQVHAVITKT-GYDSETEVCNALISMYAKCGSISDAEKQFLEVS-TKNEVSWNAIINAYSKHG---FGSEALDSFDQMIHSNVRPNHVTLVGVLSA 802          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP172_ARATH (Pentatricopeptide repeat-containing protein At2g27610 OS=Arabidopsis thaliana GN=PCMP-H60 PE=2 SV=1)

HSP 1 Score: 516.153 bits (1328), Expect = 5.126e-163
Identity = 286/828 (34.54%), Postives = 459/828 (55.43%), Query Frame = -2

Query:  922 ACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMX---XXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNM-AMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKS-GICSCQDY 3390
            A  + D+ P RD  S+ +L  G   +G   +   L+ ++ + G+  +     + LK  +   D  FG  LH + +K G L DV VG+SLVD Y K    +   +VF  M ++NVV+W  L++GYA      ++L LF  M     + + FT +  L           GL  H++V+K G +    V   L++LY KCG       +F + +  ++V W++MI      G   EA  +F  MR   V  ++ +FAS+  + ANL +  + E +H  + K+GF  +  +  AL+  Y K  ++ +  R+FKE+    +  S +         +GK  +    +F +M  +G +P+  T++ IL +    L +    +VHA ++K   + ++ VGT+L+D Y K G + +A  +F+ + ++D+ +W+A++AGYAQ+  +E AIK F  + + G+ PN FT ++ L+ C+   A +  G+  H  AIK+     + VSS+++ MYA  G IE AE  F      D VSWN M+ GY QH Q +K L+ F+ M  + ++ D VTFI V +AC++ G V EG++YFD +     I PT +H +CM+D+  RAG+  +    I++M   +   IW T+L  CR+H   +LG  AAEK+  + P  ++ Y+LL+N++A  G W++  +VR LM    VKKEPG SW+ V  + + F + D  HP   +IY+KL++L  +    GY P T ++L D+ D+ K   L  H+ERLA+AF LI+      + I KNL +C DCH  +KL++ + ++EIVVRD+N FHHF S G+CSC D+
Sbjct:   46 AHNLFDKSPGRDRESYISLLFGFSRDGRTQEAKRLFLNIHRLGMEMDCSIFSSVLKVSATLCDELFGRQLHCQCIKFGFLDDVSVGTSLVDTYMKGSNFKDGRKVFDEMKERNVVTWTTLISGYARNSMNDEVLTLFMRMQNEGTQPNSFTFAAALGVLAEEGVGGRGLQVHTVVVKNGLDKTIPVSNSLINLYLKCGNVRKARILFDKTEVKSVVTWNSMISGYAANGLDLEALGMFYSMRLNYVRLSESSFASVIKLCANLKELRFTEQLHCSVVKYGFLFDQNIRTALMVAYSKCTAMLDALRLFKEIGCVGNVVSWTAMISGFLQNDGK--EEAVDLFSEMKRKGVRPNEFTYSVILTA----LPVISPSEVHAQVVKTNYERSSTVGTALLDAYVKLGKVEEAAKVFSGIDDKDIVAWSAMLAGYAQTGETEAAIKMFGELTKGGIKPNEFTFSSILNVCAATNASMGQGKQFHGFAIKSRLDSSLCVSSALLTMYAKKGNIESAEEVFKRQREKDLVSWNSMISGYAQHGQAMKALDVFKEMKKRKVKMDGVTFIGVFAACTHAGLVEEGEKYFDIMVRDCKIAPTKEHNSCMVDLYSRAGQLEKAMKVIENMPNPAGSTIWRTILAACRVHKKTELGRLAAEKIIAMKPEDSAAYVLLSNMYAESGDWQERAKVRKLMNERNVKKEPGYSWIEVKNKTYSFLAGDRSHPLKDQIYMKLEDLSTRLKDLGYEPDTSYVLQDIDDEHKEAVLAQHSERLAIAFGLIATPKGSPLLIIKNLRVCGDCHLVIKLIAKIEEREIVVRDSNRFHHFSSDGVCSCGDF 867          

HSP 2 Score: 254.218 bits (648), Expect = 5.261e-69
Identity = 182/620 (29.35%), Postives = 310/620 (50.00%), Query Frame = -2

Query: 1708 LYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNL-LNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSS-IVDMYANCGCIEEAEAAFLGMVS-TDTVSWNMMLFGYLQHRQGLKVLETFRMMLDK--GLEPDEVTFIAVLS 3546
            ++S +L   A+       + +H + IK GF  D+ +  SLV+ Y K   F    KV D M ER+VV+WT L SG        +VL L+  M+ +G  PN      AL   +       G  +H   VK G+   + V +SL++LY KCG +  A  +F   + K+VV+WN++++GYA  G   + L +F  M  + +R S+ + ++V+K C  L  L +    H  V+K G   D+ +   L+  YSKC    D L++FK I    N+V+W+AMI    +    +EA +LFS M+ KGV PN+ T++ I      +        VHA + K  +E    +  AL+  Y+K+  V+   +VF  +   +  +    L   +Q G+T +    +F ++   G KP+  TF+SIL  C+    ++  G Q H   IK+ L  +  V ++L+ +YAK G +  AE +F +  E+DL SW ++I+GYAQ   + KA+  F  M+++ V  +  T     ++C++  ++E G+    + ++          +S +VD+Y+  G +E+A      M +   +  W  +L     H++     E  R+  +K   ++P++     +LS
Sbjct:   95 IFSSVLKVSATLCDELFGRQLHCQCIKFGFLDDVSVGTSLVDTYMKGSNFKDGRKVFDEMKERNVVTWTTLISGYARNSMNDEVLTLFMRMQNEGTQPNSFTFAAALGVLAEEGVGGRGLQVHTVVVKNGLDKTIPVSNSLINLYLKCGNVRKARILFDKTEVKSVVTWNSMISGYAANGLDLEALGMFYSMRLNYVRLSESSFASVIKLCANLKELRFTEQLHCSVVKYGFLFDQNIRTALMVAYSKCTAMLDALRLFKEIGCVGNVVSWTAMISGFLQNDGKEEAVDLFSEMKRKGVRPNEFTYSVILTALPVISP----SEVHAQVVKTNYERSSTVGTALLDAYVKLGKVEEAAKVFSGIDDKDIVAWSAMLAGYAQTGET-EAAIKMFGELTKGGIKPNEFTFSSILNVCAATNASMGQGKQFHGFAIKSRLDSSLCVSSALLTMYAKKGNIESAEEVFKRQREKDLVSWNSMISGYAQHGQAMKALDVFKEMKKRKVKMDGVTFIGVFAACTHAGLVEEGEKYFDIMVRDCKIAPTKEHNSCMVDLYSRAGQLEKAMKVIENMPNPAGSTIWRTILAACRVHKK----TELGRLAAEKIIAMKPEDSAAYVLLS 705          

HSP 3 Score: 70.4774 bits (171), Expect = 8.832e-11
Identity = 63/288 (21.88%), Postives = 125/288 (43.40%), Query Frame = -2

Query: 1480 FTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLY----AKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGND 2331
            FT+ +    +L N K   +++A+            G + + +Y      +  L +A  LF +   RD  S+ +L+ G+++   +++A + F ++ R G+  +    ++ L   + +     G+ LH   IK G   DV V +S+VD Y      ++    F  M   + V+W  ++ GY ++    +VL  F  M ++G +P+  TF A L   +  G    G +    +    G+  TI     +I++  + G   +        +V S  + W +++     +G D
Sbjct:    3 FTTTIWRPPSLENFKPKFRIYAN------------GVAQVRIYCFGTVSSSRLYNAHNLFDKSPGRDRESYISLLFGFSRDGRTQEAKRLFLNIHRLGMEMDCSIFSSVLKVSATLCDELFGRQLHCQCIKFGFLDDVSVGTSLVDTYMKGSNFKDGRKVFDEMKERNVVTWTTLISGYARNSMNDEVLTLFMRMQNEGTQPNSFTFAAALGVLAEEGVGGRGLQVHTVVVKN-GLDKTIPVSNSLINLYLKCGNVRKARILFDKTEVKS-VVTWNSMISGYAANGLD 276          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP348_ARATH (Pentatricopeptide repeat-containing protein At4g33990 OS=Arabidopsis thaliana GN=EMB2758 PE=3 SV=2)

HSP 1 Score: 473.396 bits (1217), Expect = 1.756e-147
Identity = 263/757 (34.74%), Postives = 423/757 (55.88%), Query Frame = -2

Query:  922 LHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQ-GMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNG-LKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQG-VTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3183
            LH   V    + +V + + LV+LY   G + LA   F  +  ++V +WN +++GY   G++ +++  F   M  S L     T  +VLK+C  +++   G   H L +K G   D +V   L+ LYS+     +   +F  +   ++ +W+AMI    + G  +EA  L + +R    + +  T  S+       GD      +H+   KHG ESE+ +SN LI +Y +   +++  +VF  M   +  SWNS++   +  +   R  S+F +M     +PD  T  S+    S L +I+    V    ++ G   ++  +G +++ +YAK G +  A  +F  L   D+ SW  +I+GYAQ+  + +AI+ +N M+ +G +  N  T  + L +CS    L  G  LH   +K G   DVFV +S+ DMY  CG +E+A + F  +   ++V WN ++  +  H  G K +  F+ MLD+G++PD +TF+ +LSACS+ G V+EG+  F+ +   +GI P++KH  CM+D+ GRAG+      FIK M +  +  IW  +L  CR+HGN  LG+ A+E LFE+ P    +++LL+N++A+ G+WE V  +R++    G++K PG S + V+ +V +F + +  HP + E+Y +L  L  K  + GYVP    +L DV DDEK   L  H+ERLA+AFALI+     TIRIFKNL +C DCH   K +S + ++EI+VRD+N FHHFK+G+CSC DY
Sbjct:   73 LHARLVVSKQIQNVCISAKLVNLYCYLGNVALARHTFDHIQNRDVYAWNLMISGYGRAGNSSEVIRCFSLFMLSSGLTPDYRTFPSVLKACRTVID---GNKIHCLALKFGFMWDVYVAASLIHLYSRYKAVGNARILFDEMPVRDMGSWNAMISGYCQSGNAKEALTLSNGLR----AMDSVTVVSLLSACTEAGDFNRGVTIHSYSIKHGLESELFVSNKLIDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDCLTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALRQGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHGFHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHSGLVDEGQWCFEMMQTDYGITPSLKHYGCMVDMYGRAGQLETALKFIKSMSLQPDASIWGALLSACRVHGNVDLGKIASEHLFEVEPEHVGYHVLLSNMYASAGKWEGVDEIRSIAHGKGLRKTPGWSSMEVDNKVEVFYTGNQTHPMYEEMYRELTALQAKLKMIGYVPDHRFVLQDVEDDEKEHILMSHSERLAIAFALIATPAKTTIRIFKNLRVCGDCHSVTKFISKITEREIIVRDSNRFHHFKNGVCSCGDY 822          

HSP 2 Score: 239.965 bits (611), Expect = 2.214e-64
Identity = 187/653 (28.64%), Postives = 309/653 (47.32%), Query Frame = -2

Query: 1564 SLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYC-SMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHG-FESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEG-FKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQL-LHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPT-IKHCACMIDILGRAGKFNEVE 3507
            +L SAK +H +++      ++ +   LVN Y   G    A    D +  RDV +W  + SG    G   +V+  +   M   G+ P+     + LKAC   +D   G  +H   +K G + DV+V +SL+ LY++   +  A  +F  M  +++ SWNA+++GY   G+A + L L  G+   +      T+ ++L +CT   +   G+  HS  IK G E + FV   L+DLY++ G   D  KVF R+   ++++W+++I   +   Q   A  LF  MR   + P+  T  S+A + + LGD   C  V     + G F  +I + NA++ MY K+  V +   VF  + + +  SWN+++SG            +++ M  EG    +  T+ S+L +CS    ++ G ++H  ++KNGL  +  V TSL D+Y K G L DA  LF Q+   +   W  LIA +      EKA+  F  M  +GV P++ T  T LS+CS+  +++ GQ     M    G +  +     +VDMY   G +E A                            LK +++        L+PD   + A+LSAC   G V+ GK   + L   F + P  + +   + ++   AGK+  V+
Sbjct:   66 NLQSAKCLHARLVVSKQIQNVCISAKLVNLYCYLGNVALARHTFDHIQNRDVYAWNLMISGYGRAGNSSEVIRCFSLFMLSSGLTPDYRTFPSVLKACRTVID---GNKIHCLALKFGFMWDVYVAASLIHLYSRYKAVGNARILFDEMPVRDMGSWNAMISGYCQSGNAKEALTLSNGLRAMD----SVTVVSLLSACTEAGDFNRGVTIHSYSIKHGLESELFVSNKLIDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDCLTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALRQGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHGFHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHSGLVDEGQWCFEMMQTDYGITPSLKHYGCMVDMYGRAGQLETA----------------------------LKFIKSM------SLQPDASIWGALLSACRVHGNVDLGKIASEHL---FEVEPEHVGYHVLLSNMYASAGKWEGVD 674          

HSP 3 Score: 173.326 bits (438), Expect = 7.960e-43
Identity = 137/513 (26.71%), Postives = 245/513 (47.76%), Query Frame = -2

Query: 1390 YLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSL-MRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKP-DICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNS-GDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKG-LEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVES-FIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFE-LNPGIASHYILLANIFAA 2910
            Y  NL      H+ ++ +    +  +   L++LY   G        F  IQ  ++ AW+ MI      G   E    FSL M   G++P+  TF S+      + D      +H    K GF  ++ ++ +LI +Y + ++V N   +F EM   +  SWN+++SG      C  G +     L+ G +  D  T  S+L +C+   +   G  +H++ IK+GL+    V   LIDLYA+ G L D + +F ++  RDL SW ++I  Y  ++   +AI  F  M+   + P+  TL +  S  S +  +   + +    ++ G    D+ + +++V MYA  G ++ A A F  + +TD +SWN ++ GY Q+    + +E + +M ++G +  ++ T+++VL ACS  G + +G +    L    G+   +     + D+ G+ G+  +  S F +  +V S P  W T++     HG+   GE A     E L+ G+   +I    + +A
Sbjct:   63 YCTNLQSAKCLHARLVVSKQIQNVCISAKLVNLYCYLGNVALARHTFDHIQNRDVYAWNLMISGYGRAGNSSEVIRCFSLFMLSSGLTPDYRTFPSVLKACRTVID---GNKIHCLALKFGFMWDVYVAASLIHLYSRYKAVGNARILFDEMPVRDMGSWNAMISGY-----CQSGNAKEALTLSNGLRAMDSVTVVSLLSACTEAGDFNRGVTIHSYSIKHGLESELFVSNKLIDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDCLTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALRQGMKLHGRLLKN-GLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVP--WNTLIACHGFHGH---GEKAVMLFKEMLDEGVKPDHITFVTLLSA 561          

HSP 4 Score: 149.443 bits (376), Expect = 3.041e-35
Identity = 92/314 (29.30%), Postives = 157/314 (50.00%), Query Frame = -2

Query: 2599 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLL-DVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTES-ELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLG 3534
            +L  C   G  +    +H   IK G + +L + N L++ YA+ G      KV DRM  RD++SW ++             + L+  M+   + P+   L++     S   D+     + G  ++ G  L D+ +G+++V +YAK G ++ A  VF ++   +V+SWN +++GYA  G A + + ++  M E  E+  ++ T  +VL +C+    L  G+  H  ++K G   D FV   L D+Y KCG  ED L +F +I   N V W+ +I C    G  ++A  LF  M  +GV P+  TF ++    ++ G
Sbjct:  253 LLSACTEAGDFNRGVTIHSYSIKHGLESELFVSNKLIDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDCLTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALRQGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHGFHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHSG 566          

HSP 5 Score: 132.88 bits (333), Expect = 3.924e-30
Identity = 83/279 (29.75%), Postives = 144/279 (51.61%), Query Frame = -2

Query: 2698 GSLSSAKAVHGKIIKKG-FDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDG-VLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTY--LVN---LTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCLDEQGQI 3510
            G + + ++V G  ++KG F  D+ + N++V  YAK G+   A  V + +P  DV+SW  + SG    G+  + +++Y  M+++G +  N    V+ L ACS +  L  G  LHG  +K G+ LDVFV +SL D+Y KCG++E A  +F  + + N V WN L+  +   G   K + LF+ M +  ++    T   +L +C++  LV+     + ++     I    +H      C++D+Y + G  E  LK  K +  +P+   W A++      G +
Sbjct:  362 GDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALRQGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHGFHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHSGLVDEGQWCFEMMQTDYGITPSLKHYG----CMVDMYGRAGQLETALKFIKSMSLQPDASIWGALLSACRVHGNV 636          

HSP 6 Score: 70.0922 bits (170), Expect = 1.128e-10
Identity = 61/218 (27.98%), Postives = 96/218 (44.04%), Query Frame = -2

Query: 2917 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSL------VDLYAKCGQMELAERVFLFMD-KKNVVSWNALLNGYALKG--DAGKILNLFQGMTESELRCSKFTLSNVLKS 3543
            +  +L  C+  G+L     +HG+++K G   D+ +  SL + Y K G    A  +  ++P  + V W  L +     G+G   + L+  M  +GV P+    VT L ACS S     G +  G+     +  D  +  SL      VD+Y + GQ+E A +    M  + +   W ALL+   + G  D GKI +      E E       LSN+  S
Sbjct:  454 WVSVLPACSQAGALRQGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHGFHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHS-----GLVDEGQWCFEMMQTDYGITPSLKHYGCMVDMYGRAGQLETALKFIKSMSLQPDASIWGALLSACRVHGNVDLGKIASEHLFEVEPEHVGYHVLLSNMYAS 666          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP347_ARATH (Pentatricopeptide repeat-containing protein At4g33170 OS=Arabidopsis thaliana GN=PCMP-H53 PE=3 SV=1)

HSP 1 Score: 476.093 bits (1224), Expect = 1.653e-146
Identity = 281/875 (32.11%), Postives = 461/875 (52.69%), Query Frame = -2

Query:  922 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELF-SLMRCKGVSPNQHTFASIAGVAANLGDRL-YCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3540
            S +L  C   G + ++++ HG   K G D D  +  +LVN Y K G       + + MP RDVV W  +    +  G+  + +DL  +    G+ PN            ++L L     + G+    G +     G+    +                     ++  N  L+ Y   G    +L  F  M ES++ C + T   +L +   + +L  G   H + +K G +    V   L+++Y K         VF  + E ++++W+++I  + + G   EA  LF  L+RC G+ P+Q+T  S+   A++L + L   + VH    K    S+  +S ALI  Y + R ++    +F+  +    A    +   +Q+     +   +F  M  +G + D  T  ++ ++C  L  I  G QVHA+ IK+G   +  V + ++D+Y K G ++ A+  F  +   D  +WT +I+G  ++   E+A   F+ M+  GV P+ FT+AT   + S +  LE G+ +H+ A+K   + D FV +S+VDMYA CG I++A   F  +   +  +WN ML G  QH +G + L+ F+ M   G++PD+VTFI VLSACS+ G V+E  ++  S+   +GI P I+H +C+ D LGRAG   + E+ I+ M + ++  ++ T+L  CR+ G+ + G+  A KL EL P  +S Y+LL+N++AA  +W++++  R +M    VKK+PG SW+ V  ++HIF   D  + +   IY K+K+++      GYVP+TD  L DV ++EK   L++H+E+LA+AF L+S  P   IR+ KNL +C DCH+ MK ++ V  +EIV+RDAN FH FK GICSC DY
Sbjct:  149 SPMLKLCLHSGYVWASESFHGYACKIGLDGDEFVAGALVNIYLKFGKVKEGKVLFEEMPYRDVVLWNLMLKAYLEMGFKEEAIDLSSAFHSSGLNPN-----------EITLRLL--ARISGDDSDAGQVKSFANGNDASSV-------------------SEIIFRNKGLSEYLHSGQYSALLKCFADMVESDVECDQVTFILMLATAVKVDSLALGQQVHCMALKLGLDLMLTVSNSLINMYCKLRKFGFARTVFDNMSERDLISWNSVIAGIAQNGLEVEAVCLFMQLLRC-GLKPDQYTMTSVLKAASSLPEGLSLSKQVHVHAIKINNVSDSFVSTALIDAYSRNRCMKEAEILFERHNFDLVAWNAMMAGYTQSHDG-HKTLKLFALMHKQGERSDDFTLATVFKTCGFLFAINQGKQVHAYAIKSGYDLDLWVSSGILDMYVKCGDMSAAQFAFDSIPVPDDVAWTTMISGCIENGEEERAFHVFSQMRLMGVLPDEFTIATLAKASSCLTALEQGRQIHANALKLNCTNDPFVGTSLVDMYAKCGSIDDAYCLFKRIEMMNITAWNAMLVGLAQHGEGKETLQLFKQMKSLGIKPDKVTFIGVLSACSHSGLVSEAYKHMRSMHGDYGIKPEIEHYSCLADALGRAGLVKQAENLIESMSMEASASMYRTLLAACRVQGDTETGKRVATKLLELEPLDSSAYVLLSNMYAAASKWDEMKLARTMMKGHKVKKDPGFSWIEVKNKIHIFVVDDRSNRQTELIYRKVKDMIRDIKQEGYVPETDFTLVDVEEEEKERALYYHSEKLAVAFGLLSTPPSTPIRVIKNLRVCGDCHNAMKYIAKVYNREIVLRDANRFHRFKDGICSCGDY 989          

HSP 2 Score: 234.572 bits (597), Expect = 4.446e-62
Identity = 193/697 (27.69%), Postives = 329/697 (47.20%), Query Frame = -2

Query: 1477 LHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGL------VNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLN-IKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIER---DLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKE-YFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDK 3531
            L +  +   L   K  H +I+    +P+  L N+L++ Y+K G   YA +V D+MP+RD+VSW ++ +        V E      L L+  +++D V  +   L   LK C  S  ++     HG   K+G+  D FV  +LV++Y K G+++  + +F  M  ++VV WN +L  Y   G   + ++L      S L  ++ TL         L+    G  + +  +K+    ++      + ++   GL+E            +   +SA++ C             F+ M    V  +Q TF  +   A  +      + VH    K G +  + +SN+LI MY K+R       VF  MS  +  S  + +   +QNG   +    +F ++L  G KPD  T TS+L++ S+L   +    QVH H IK     ++ V T+LID Y++N  + +AE+LF    ER   DL +W A++AGY QS    K +K F  M +QG   ++FTLAT   +C  +  +  G+ +H+ AIK+G   D++VSS I+DMY  CG +  A+ AF  +   D V+W  M+ G +++ +  +    F  M   G+ PDE T   +  A S +  + +G++ + ++L       P +     ++D+  + G  ++     K +++  N   W  +L     HG  K
Sbjct:   46 LRNAITSSDLMLGKCTHARILTFEENPERFLINNLISMYSKCGSLTYARRVFDKMPDRDLVSWNSILAAYAQSSECVVENIQQAFL-LFRILRQDVVYTSRMTLSPMLKLCLHSGYVWASESFHGYACKIGLDGDEFVAGALVNIYLKFGKVKEGKVLFEEMPYRDVVLWNLMLKAYLEMGFKEEAIDLSSAFHSSGLNPNEITLR--------LLARISGDDSDAGQVKSFANGNDASSVSEI-IFRNKGLSEYL----------HSGQYSALLKC-------------FADMVESDVECDQVTFILMLATAVKVDSLALGQQVHCMALKLGLDLMLTVSNSLINMYCKLRKFGFARTVFDNMSERDLISWNSVIAGIAQNGLEVE-AVCLFMQLLRCGLKPDQYTMTSVLKAASSLPEGLSLSKQVHVHAIKINNVSDSFVSTALIDAYSRNRCMKEAEILF----ERHNFDLVAWNAMMAGYTQSHDGHKTLKLFALMHKQGERSDDFTLATVFKTCGFLFAINQGKQVHAYAIKSGYDLDLWVSSGILDMYVKCGDMSAAQFAFDSIPVPDDVAWTTMISGCIENGEEERAFHVFSQMRLMGVLPDEFTIATLAKASSCLTALEQGRQIHANALKLNCTNDPFVG--TSLVDMYAKCGSIDDAYCLFKRIEMM-NITAWNAMLVGLAQHGEGK 701          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP373_ARATH (Putative pentatricopeptide repeat-containing protein At5g09950 OS=Arabidopsis thaliana GN=PCMP-H35 PE=3 SV=1)

HSP 1 Score: 473.396 bits (1217), Expect = 1.964e-145
Identity = 309/892 (34.64%), Postives = 480/892 (53.81%), Query Frame = -2

Query:  922 YSKILHDCASKGSLSS--AKAVHGKIIKKGFDPDLHLWNSLVNAYAK-RGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHC---LVTALKACSLS-LDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKG---DAGKILNLFQGMTESELRCSKFTLSNVLK-SCTYLVNLTWGLIAHSLVIKTG-CEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIER-DLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMM-LDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTC-RMHGND-KLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISI-NPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3543
            +  +L  C   GS+     + +HG + K  +  D  + N L++ Y K  G  GYA      +  ++ VSW ++ S     G       ++ SM+ DG  P  +    LVT   ACSL+  D+     +     K G+L D+FVGS LV  +AK G +  A +VF  M+ +N V+ N L+ G   +    +A K+      M +         LS+  + S    V L  G   H  VI TG  +    +G  L+++Y+KCG   D  +VF  + + + V+W++MI  LD+ G   EA E +  MR   + P   T  S     A+L      + +H    K G +  + +SNAL+ +Y +   +    ++F  M   +  S  + + + +++ ++       F      G K +  TF+S+L + S+L   + G Q+H   +KN + D      +LI  Y K G +   E +F+++ ER D  +W ++I+GY  ++   KA+     M + G   ++F  AT LS+ +++A LE G  +H+ +++A    DV V S++VDMY+ CG ++ A   F  M   ++ SWN M+ GY +H QG + L+ F  M LD    PD VTF+ VLSACS+ G + EG ++F+S+++ +G+ P I+H +CM D+LGRAG+ +++E FI+ M +  N LIW TVLG C R +G   +LG+ AAE LF+L P  A +Y+LL N++AA GRWED+ + R  M    VKKE G SWV +   VH+F + D  HP    IY KLKEL  K   AGYVP+T   L D+  + K E L +H+E+LA+AF L +  +    IRI KNL +C DCH   K +S +  ++I++RD+N FHHF+ G CSC D+
Sbjct:  105 FVSVLRACQEIGSVGILFGRQIHGLMFKLSYAVDAVVSNVLISMYWKCIGSVGYALCAFGDIEVKNSVSWNSIISVYSQAGDQRSAFRIFSSMQYDGSRPTEYTFGSLVTT--ACSLTEPDVRLLEQIMCTIQKSGLLTDLFVGSGLVSAFAKSGSLSYARKVFNQMETRNAVTLNGLMVGLVRQKWGEEATKLFMDMNSMIDVSPESYVILLSSFPEYSLAEEVGLKKGREVHGHVITTGLVDFMVGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLDQNGCFIEAVERYKSMRRHDILPGSFTLISSLSSCASLKWAKLGQQIHGESLKLGIDLNVSVSNALMTLYAETGYLNECRKIFSSMPEHDQVSWNSIIGALARSERSLPEAVVCFLNAQRAGQKLNRITFSSVLSAVSSLSFGELGKQIHGLALKNNIADEATTENALIACYGKCGEMDGCEKIFSRMAERRDNVTWNSMISGYIHNELLAKALDLVWFMLQTGQRLDSFMYATVLSAFASVATLERGMEVHACSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPVRNSYSWNSMISGYARHGQGEEALKLFETMKLDGQTPPDHVTFVGVLSACSHAGLLEEGFKHFESMSDSYGLAPRIEHFSCMADVLGRAGELDKLEDFIEKMPMKPNVLIWRTVLGACCRANGRKAELGKKAAEMLFQLEPENAVNYVLLGNMYAAGGRWEDLVKARKKMKDADVKKEAGYSWVTMKDGVHMFVAGDKSHPDADVIYKKLKELNRKMRDAGYVPQTGFALYDLEQENKEEILSYHSEKLAVAFVLAAQRSSTLPIRIMKNLRVCGDCHSAFKYISKIEGRQIILRDSNRFHHFQDGACSCSDF 994          

HSP 2 Score: 240.736 bits (613), Expect = 5.697e-64
Identity = 198/709 (27.93%), Postives = 333/709 (46.97%), Query Frame = -2

Query: 1444 GSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSL--SLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKC-GQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSL--VIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCK-GVSPNQHTFASIAGVAANLGDRLYCEG---VHACIFKHGFESEII-LSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCS-EKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVS-TDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHC--------ACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFE 3510
            G   +A+  H ++ K   D D++L N+L+NAY + G    A KV D MP R+ VSW  + SG    G   + L     M K+G+  N +  V+ L+AC    S+ + FG  +HG   K+   +D  V + L+ +Y KC G +  A   F  ++ KN VSWN++++ Y+  GD      +F  M     R +++T  +++ +   L      L+   +  + K+G   D FVG  L+  ++K G      KVF +++  N V  + ++  L  Q   +EA +LF  M     VSP  +     +    +L + +  +    VH  +   G    ++ + N L+ MY K  S+ +  RVF  M+  +S SWNS+++G             +  M      P   T  S L SC++L   K G Q+H   +K G+  N  V  +L+ LYA+ G+L +   +F+ + E D  SW ++I   A+S+ S  +A+ CF + QR G   N  T ++ LS+ S+++  E G+ +H +A+K   + +    ++++  Y  CG ++  E  F  M    D V+WN M+ GY+ +    K L+    ML  G   D   +  VLSA + +  +  G E             +++ C        + ++D+  + G+ +    F   M V  N   W +++     HG    GE A  KLFE
Sbjct:   15 GHRGAARFFHSRLYKNRLDKDVYLCNNLINAYLETGDSVSARKVFDEMPLRNCVSWACIVSGYSRNGEHKEALVFLRDMVKEGIFSNQYAFVSVLRACQEIGSVGILFGRQIHGLMFKLSYAVDAVVSNVLISMYWKCIGSVGYALCAFGDIEVKNSVSWNSIISVYSQAGDQRSAFRIFSSMQYDGSRPTEYTFGSLVTTACSLTEPDVRLLEQIMCTIQKSGLLTDLFVGSGLVSAFAKSGSLSYARKVFNQMETRNAVTLNGLMVGLVRQKWGEEATKLFMDMNSMIDVSPESYVILLSSFPEYSLAEEVGLKKGREVHGHVITTGLVDFMVGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLDQNGCFIEAVERYKSMRRHDILPGSFTLISSLSSCASLKWAKLGQQIHGESLKLGIDLNVSVSNALMTLYAETGYLNECRKIFSSMPEHDQVSWNSIIGALARSERSLPEAVVCFLNAQRAGQKLNRITFSSVLSAVSSLSFGELGKQIHGLALKNNIADEATTENALIACYGKCGEMDGCEKIFSRMAERRDNVTWNSMISGYIHNELLAKALDLVWFMLQTGQRLDSFMYATVLSAFASVATLERGMEVH---------ACSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPV-RNSYSWNSMISGYARHGQ---GEEAL-KLFE 709          

HSP 3 Score: 71.633 bits (174), Expect = 4.270e-11
Identity = 54/187 (28.88%), Postives = 89/187 (47.59%), Query Frame = -2

Query: 3028 LEGAKRLK--LYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGH-CLVTALKACSLSLDLFFGTLLHGEGV--KVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMD-KKNVVSWNALL 3570
            L+  +RL   +Y+ +L   AS  +L     VH   ++   + D+ + ++LV+ Y+K G   YA +  + MP R+  SW ++ SG    G G + L L+ +MK DG  P  H   V  L ACS +  L      H E +    G+   +   S + D+  + G+++  E     M  K NV+ W  +L
Sbjct:  611 LQTGQRLDSFMYATVLSAFASVATLERGMEVHACSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPVRNSYSWNSMISGYARHGQGEEALKLFETMKLDGQTPPDHVTFVGVLSACSHA-GLLEEGFKHFESMSDSYGLAPRIEHFSCMADVLGRAGELDKLEDFIEKMPMKPNVLIWRTVL 796          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP296_ARATH (Pentatricopeptide repeat-containing protein At3g63370, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H83 PE=2 SV=2)

HSP 1 Score: 471.855 bits (1213), Expect = 3.273e-145
Identity = 278/886 (31.38%), Postives = 472/886 (53.27%), Query Frame = -2

Query:  925 EGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIK--KGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFL-FMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGC-CLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEK-SILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQD 3567
            E    ++ ++ +L  C  + ++S  + +H +I K    F+ D  L   LV  Y K G    A KV D MP+R   +W  +    V+ G     L LY +M+ +GV          LKAC+   D+  G+ LH   VK+G     F+ ++LV +YAK   +  A R+F  F +K + V WN++L+ Y+  G + + L LF+ M  +    + +T+ + L +C        G   H+ V+K+     E   C  L+ +Y++CG      ++ +++   ++V W+++I    +    +EA E FS M   G   ++ +  SI   +  L + L    +HA + KHG++S + + N LI MY K        R F  M   +  SW ++++G            +F  +  +  + D     SILR+ S L ++    ++H HI++ GL D  ++   L+D+Y K   +  A  +F  +  +D+ SWT++I+  A +    +A++ F  M   G++ ++  L   LS+ ++++ L  G+ +H   ++ G   +  ++ ++VDMYA CG ++ A+A F  +     + +  M+  Y  H  G   +E F  M  + + PD ++F+A+L ACS+ G ++EG+ +   + + + + P  +H  C++D+LGRA    E   F+K MK      +W  +L  CR H   ++GE AA++L EL P    + +L++N+FA +GRW DV +VRA M   G++K PGCSW+ ++G+VH F + D  HP+  EIY KL E+  K     GYV  T  +L++V + EK++ L  H+ER+A+A+ L+       +RI KNL +C DCH F KLVS + +++IV+RDAN FHHF+SG+CSC D
Sbjct:   75 ENNSPVEAFAYVLELCGKRRAVSQGRQLHSRIFKTFPSFELDF-LAGKLVFMYGKCGSLDDAEKVFDEMPDRTAFAWNTMIGAYVSNGEPASALALYWNMRVEGVPLGLSSFPALLKACAKLRDIRSGSELHSLLVKLGYHSTGFIVNALVSMYAKNDDLSAARRLFDGFQEKGDAVLWNSILSSYSTSGKSLETLELFREMHMTGPAPNSYTIVSALTACDGFSYAKLGKEIHASVLKSSTHSSELYVCNALIAMYTRCGKMPQAERILRQMNNADVVTWNSLIKGYVQNLMYKEALEFFSDMIAAGHKSDEVSMTSIIAASGRLSNLLAGMELHAYVIKHGWDSNLQVGNTLIDMYSKCNLTCYMGRAFLRMHDKDLISWTTVIAGYAQNDCHVEALELFRDVAKKRMEIDEMILGSILRASSVLKSMLIVKEIHCHILRKGLLDT-VIQNELVDVYGKCRNMGYATRVFESIKGKDVVSWTSMISSSALNGNESEAVELFRRMVETGLSADSVALLCILSAAASLSALNKGREIHCYLLRKGFCLEGSIAVAVVDMYACCGDLQSAKAVFDRIERKGLLQYTSMINAYGMHGCGKAAVELFDKMRHENVSPDHISFLALLYACSHAGLLDEGRGFLKIMEHEYELEPWPEHYVCLVDMLGRANCVVEAFEFVKMMKTEPTAEVWCALLAACRSHSEKEIGEIAAQRLLELEPKNPGNLVLVSNVFAEQGRWNDVEKVRAKMKASGMEKHPGCSWIEMDGKVHKFTARDKSHPESKEIYEKLSEVTRKLEREVGYVADTKFVLHNVDEGEKVQMLHGHSERIAIAYGLLRTPDRACLRITKNLRVCRDCHTFCKLVSKLFRRDIVMRDANRFHHFESGLCSCGD 958          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP272_ARATH (Pentatricopeptide repeat-containing protein At3g49170, chloroplastic OS=Arabidopsis thaliana GN=EMB2261 PE=2 SV=1)

HSP 1 Score: 465.692 bits (1197), Expect = 2.857e-144
Identity = 270/806 (33.50%), Postives = 445/806 (55.21%), Query Frame = -2

Query:  922 LDLYCSMKKDGVLP-NGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMD---KKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTG-CEHDEFVGCCLLDLYSKCGLA-EDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIR---SVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEG-FKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQ----LFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3294
            LDL   M +DG+ P +     + LK+C  + D   G L+H   ++  +  D  + +SL+ LY+K G    AE VF  M    K++VVSW+A++  Y   G     + +F    E  L  + +  + V+++C+    +  G +    ++KTG  E D  VGC L+D++ K   + E+  KVF ++ E N+V W+ MI    + G  +EA   F  M   G   ++ T +S+    A L +    + +H+   + G   ++  S  L+ MY K     SV +  +VF  M   +  S    +    +N        ++F +M+ +G  +P+  TF+S  ++C NL + + G QV     K GL  N+ V  S+I ++ K+  + DA+  F  L E++L S+   + G  ++   E+A K  + +  + +  + FT A+ LS  +N+  +  G+ +HS  +K G S +  V ++++ MY+ CG I+ A   F  M + + +SW  M+ G+ +H   ++VLETF  M+++G++P+EVT++A+LSACS++G V+EG  +F+S+     I P ++H ACM+D+L RAG   +   FI  M   ++ L+W T LG CR+H N +LG+ AA K+ EL+P   + YI L+NI+A  G+WE+   +R  M    + KE GCSW+ V  ++H F   D  HP   +IY +L  L+ +    GYVP TD +L+ + ++    +    L+ H+E++A+AF LIS +  + +R+FKNL +C DCH+ MK +S V  +EIV+RD N FHHFK G CSC DY
Sbjct:   49 LDL---MARDGIRPMDSVTFSSLLKSCIRARDFRLGKLVHARLIEFDIEPDSVLYNSLISLYSKSGDSAKAEDVFETMRRFGKRDVVSWSAMMACYGNNGRELDAIKVFVEFLELGLVPNDYCYTAVIRACSNSDFVGVGRVTLGFLMKTGHFESDVCVGCSLIDMFVKGENSFENAYKVFDKMSELNVVTWTLMITRCMQMGFPREAIRFFLDMVLSGFESDKFTLSSVFSACAELENLSLGKQLHSWAIRSGLVDDVECS--LVDMYAKCSADGSVDDCRKVFDRMEDHSVMSWTALITGYMKNCNLATEAINLFSEMITQGHVEPNHFTFSSAFKACGNLSDPRVGKQVLGQAFKRGLASNSSVANSVISMFVKSDRMEDAQRAFESLSEKNLVSYNTFLDGTCRNLNFEQAFKLLSEITERELGVSAFTFASLLSGVANVGSIRKGEQIHSQVVKLGLSCNQPVCNALISMYSKCGSIDTASRVFNFMENRNVISWTSMITGFAKHGFAIRVLETFNQMIEEGVKPNEVTYVAILSACSHVGLVSEGWRHFNSMYEDHKIKPKMEHYACMVDLLCRAGLLTDAFEFINTMPFQADVLVWRTFLGACRVHSNTELGKLAARKILELDPNEPAAYIQLSNIYACAGKWEESTEMRRKMKERNLVKEGGCSWIEVGDKIHKFYVGDTAHPNAHQIYDELDRLITEIKRCGYVPDTDLVLHKLEEENDEAEKERLLYQHSEKIAVAFGLISTSKSRPVRVFKNLRVCGDCHNAMKYISTVSGREIVLRDLNRFHHFKDGKCSCNDY 849          

HSP 2 Score: 251.136 bits (640), Expect = 6.102e-68
Identity = 168/532 (31.58%), Postives = 279/532 (52.44%), Query Frame = -2

Query: 1978 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLD---RMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVL-LDVFVGSSLVDLYAKC-GQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKC---GLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQI-QEAAELFSLMRCKG-VSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG 3543
            +S +L  C         K VH ++I+   +PD  L+NSL++ Y+K G    A  V +   R  +RDVVSW+A+ +   N G   D + ++    + G++PN +C    ++ACS S  +  G +  G  +K G    DV VG SL+D++ K     E A +VF  M + NVV+W  ++      G   + +  F  M  S     KFTLS+V  +C  L NL+ G   HS  I++G   D  V C L+D+Y+KC   G  +D  KVF R+++ ++++W+A+I    +   +  EA  LFS M  +G V PN  TF+S      NL D    + V    FK G  S   ++N++I+M++K   +++  R F+ +S  N  S+N+ L G+      ++   +  ++          TF S+L   +N+ +I+ G Q+H+ ++K GL  N  V  +LI +Y+K G +  A  +F  +  R++ SWT++I G+A+   + + ++ FN M  +GV PN  T    LS+CS++ ++  G
Sbjct:   65 FSSLLKSCIRARDFRLGKLVHARLIEFDIEPDSVLYNSLISLYSKSGDSAKAEDVFETMRRFGKRDVVSWSAMMACYGNNGRELDAIKVFVEFLELGLVPNDYCYTAVIRACSNSDFVGVGRVTLGFLMKTGHFESDVCVGCSLIDMFVKGENSFENAYKVFDKMSELNVVTWTLMITRCMQMGFPREAIRFFLDMVLSGFESDKFTLSSVFSACAELENLSLGKQLHSWAIRSGLVDD--VECSLVDMYAKCSADGSVDDCRKVFDRMEDHSVMSWTALITGYMKNCNLATEAINLFSEMITQGHVEPNHFTFSSAFKACGNLSDPRVGKQVLGQAFKRGLASNSSVANSVISMFVKSDRMEDAQRAFESLSEKNLVSYNTFLDGTCRNLNFEQAFKLLSEITERELGVSAFTFASLLSGVANVGSIRKGEQIHSQVVKLGLSCNQPVCNALISMYSKCGSIDTASRVFNFMENRNVISWTSMITGFAKHGFAIRVLETFNQMIEEGVKPNEVTYVAILSACSHVGLVSEG 594          

HSP 3 Score: 73.9442 bits (180), Expect = 6.574e-12
Identity = 38/126 (30.16%), Postives = 67/126 (53.17%), Query Frame = -2

Query: 3214 LFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACS 3591
            L  E T+ E       ++ +L   A+ GS+   + +H +++K G   +  + N+L++ Y+K G    A +V + M  R+V+SWT++ +G    G+   VL+ +  M ++GV PN    V  L ACS
Sbjct:  461 LLSEITERELGVSAFTFASLLSGVANVGSIRKGEQIHSQVVKLGLSCNQPVCNALISMYSKCGSIDTASRVFNFMENRNVISWTSMITGFAKHGFAIRVLETFNQMIEEGVKPNEVTYVAILSACS 586          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP320_ARATH (Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis thaliana GN=DOT4 PE=2 SV=1)

HSP 1 Score: 460.299 bits (1183), Expect = 5.934e-142
Identity = 269/820 (32.80%), Postives = 442/820 (53.90%), Query Frame = -2

Query:  922 ERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASI-AGVA----ANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHM-QRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3363
            +R V             G   + + L C   K  + P   C V  L+ C+ S  L  G  +       G ++D  +GS L  +Y  CG ++ A RVF  +  +  + WN L+N  A  GD    + LF+ M  S +    +T S V KS + L ++  G   H  ++K+G      VG  L+  Y K    +   KVF  + E ++++W+++I+     G  ++   +F  M   G+  +  T  S+ AG A     +LG  ++  GV AC     F  E    N L+ MY K   + +   VF+EMS  +  S+ S+++G            +F +M  EG  PD+ T T++L  C+    +  G +VH  I +N L  +  V  +L+D+YAK G + +AEL+F+++  +D+ SW  +I GY+++  + +A+  FN + + +  +P+  T+A  L +C++++  + G+ +H   ++ G   D  V++S+VDMYA CG +  A   F  + S D VSW +M+ GY  H  G + +  F  M   G+E DE++F+++L ACS+ G V+EG  +F+ + +   I PT++H AC++D+L R G   +   FI++M +  +  IW  +L  CR+H + KL E  AEK+FEL P    +Y+L+ANI+A   +WE V+R+R  +   G++K PGCSW+ + G+V+IF + D  +P+   I   L+++  + I  GY P T + L D  + EK E L  H+E+LA+A  +IS    K IR+ KNL +C DCH+  K +S + ++EIV+RD+N FH FK G CSC+ +
Sbjct:   58 DRSVTDANTQLRRFCESGNLENAVKLLCVSGKWDIDPRTLCSV--LQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKAC-----FSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPDATIWGALLCGCRIHHDVKLAEKVAEKVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIEIKGRVNIFVAGDSSNPETENIEAFLRKVRARMIEEGYSPLTKYALIDAEEMEKEEALCGHSEKLAMALGIISSGHGKIIRVTKNLRVCGDCHEMAKFMSKLTRREIVLRDSNRFHQFKDGHCSCRGF 870          

HSP 2 Score: 234.187 bits (596), Expect = 2.832e-62
Identity = 182/614 (29.64%), Postives = 298/614 (48.53%), Query Frame = -2

Query: 1708 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEG--VHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEG-FKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLS 3534
            +L  CA   SL   K V   I   GF  D +L + L   Y   G    A +V D +     + W  L + L   G     + L+  M   GV  + +      K+ S    +  G  LHG  +K G      VG+SLV  Y K  +++ A +VF  M +++V+SWN+++NGY   G A K L++F  M  S +     T+ +V   C     ++ G   HS+ +K     ++     LLD+YSKCG  +    VF+ + + ++V++++MI     +G   EA +LF  M  +G+SP+ +T  ++    A    RL  EG  VH  I ++    +I +SNAL+ MY K  S+Q    VF EM   +  SWN+++ G       +   S+F+ +L E  F PD  T   +L +C++L     G ++H +I++NG   +  V  SL+D+YAK G L  A +LF  +  +DL SWT +IAGY      ++AI  FN M++ G+  +  +  + L +CS+  +++ G +  + M  +      V   + IVDM A  G + +A      M +  D   W  +L G   H   +K+ E     + + LEP+   +  +++
Sbjct:  100 VLQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARY--RLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPDATIWGALLCGCRIHHD-VKLAEKVAEKVFE-LEPENTGYYVLMA 709          

HSP 3 Score: 112.464 bits (280), Expect = 1.067e-23
Identity = 82/302 (27.15%), Postives = 148/302 (49.01%), Query Frame = -2

Query: 2725 VPLFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLY-CSMKKDGVLPNGHCLVTALKACSLSLDLF-FGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTY--LVNLTW---GLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQE----PNIVAWSAMI 3597
            V LF+E  +   +  +   + +L+ CA    L   K VH  I +     D+ + N+L++ YAK G    A  V   M  +D++SW  +  G     Y  + L L+   +++    P+   +   L AC+ SL  F  G  +HG  ++ G   D  V +SLVD+YAKCG + LA  +F  +  K++VSW  ++ GY + G   + + LF  M ++ +   + +  ++L +C++  LV+  W    ++ H   I+   EH      C++D+ ++ G   D +K ++ I+     P+   W A++
Sbjct:  382 VKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACA-SLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEH----YACIVDMLARTG---DLIKAYRFIENMPIPPDATIWGALL 675          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP206_ARATH (Putative pentatricopeptide repeat-containing protein At2g01510 OS=Arabidopsis thaliana GN=PCMP-H36 PE=3 SV=1)

HSP 1 Score: 458.373 bits (1178), Expect = 9.262e-142
Identity = 258/743 (34.72%), Postives = 391/743 (52.62%), Query Frame = -2

Query:  922 SSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCS---KFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLK--VFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNP-GIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3132
            ++++  + K G +  A  +F  M  + VV+W  L+  YA      +   LF+ M  S   C+     T + +L  C   V        H+  +K G + + F+    + L S C +    L   +F+ I E + V ++ +I   ++ G   E+  LF  MR  G  P+  TF+ +      L D    + +HA     GF  +  + N ++  Y K   V     +F EM   +  S+N ++S        +     F +M   GF      F ++L   +NL +++ G Q+H   +         VG SL+D+YAK     +AEL+F  L +R   SWTALI+GY Q       +K F  M+   +  +  T AT L + ++ A L  G+ LH+  I++GN  +VF  S +VDMYA CG I++A   F  M   + VSWN ++  +  +  G   +  F  M++ GL+PD V+ + VL+ACS+ GFV +G EYF +++ ++GI P  KH ACM+D+LGR G+F E E  + +M    + ++W +VL  CR+H N  L E AAEKLF +     A+ Y+ ++NI+AA G WE VR V+  M   G+KK P  SWV VN ++H+F S D  HP   EI  K+ EL  +    GY P T  ++ DV +  K+E L +H+ERLA+AFALIS      I + KNL  C DCH  +KL+S ++K+EI VRD + FHHF  G+CSC DY
Sbjct:   83 NTMISGHVKTGDVSSARDLFDAMPDRTVVTWTILMGWYARNSHFDEAFKLFRQMCRSS-SCTLPDHVTFTTLLPGCNDAVPQNAVGQVHAFAVKLGFDTNPFLTVSNVLLKSYCEVRRLDLACVLFEEIPEKDSVTFNTLITGYEKDGLYTESIHLFLKMRQSGHQPSDFTFSGVLKAVVGLHDFALGQQLHALSVTTGFSRDASVGNQILDFYSKHDRVLETRMLFDEMPELDFVSYNVVISSYSQADQYEASLHFFREMQCMGFDRRNFPFATMLSIAANLSSLQMGRQLHCQALLATADSILHVGNSLVDMYAKCEMFEEAELIFKSLPQRTTVSWTALISGYVQKGLHGAGLKLFTKMRGSNLRADQSTFATVLKASASFASLLLGKQLHAFIIRSGNLENVFSGSGLVDMYAKCGSIKDAVQVFEEMPDRNAVSWNALISAHADNGDGEAAIGAFAKMIESGLQPDSVSILGVLTACSHCGFVEQGTEYFQAMSPIYGITPKKKHYACMLDLLGRNGRFAEAEKLMDEMPFEPDEIMWSSVLNACRIHKNQSLAERAAEKLFSMEKLRDAAAYVSMSNIYAAAGEWEKVRDVKKAMRERGIKKVPAYSWVEVNHKIHVFSSNDQTHPNGDEIVRKINELTAEIEREGYKPDTSSVVQDVDEQMKIESLKYHSERLAVAFALISTPEGCPIVVMKNLRACRDCHAAIKLISKIVKREITVRDTSRFHHFSEGVCSCGDY 824          

HSP 2 Score: 217.238 bits (552), Expect = 5.594e-57
Identity = 184/700 (26.29%), Postives = 316/700 (45.14%), Query Frame = -2

Query: 1543 SKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLP----------NGHCLVTALK---------ACSLSLDLFFGTLL---------------HGEGVKVGVLLDVF--VGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANL-----GDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMK 3516
            S  +    + V  +IIK GFD D    N +V    +RG    A KV D MP ++ VS   + SG V  G      DL+ +M    V+           N H    A K         +C+L   + F TLL               H   VK+G   + F  V + L+  Y +  +++LA  +F  + +K+ V++N L+ GY   G   + ++LF  M +S  + S FT S VLK+   L +   G   H+L + TG   D  VG  +LD YSK     +T  +F  + E + V+++ +I    +  Q + +   F  M+C G       FA++  +AANL     G +L+C+ + A       +S + + N+L+ MY K    +    +FK +    + SW +L+SG         G  +F KM     + D  TF ++L++ ++  ++  G Q+HA II++G  +N   G+ L+D+YAK G + DA  +F ++ +R+  SW ALI+ +A +   E AI  F  M   G+ P++ ++   L++CS+   +E G +   +M+   G +      + ++D+    G   EAE                                  ++M +   EPDE+ + +VL+AC      +  +   + L ++  +     + + M +I   AG++ +V    K M+
Sbjct:   25 SPATFLDTRRVDARIIKTGFDTDTCRSNFIVEDLLRRGQVSAARKVYDEMPHKNTVSTNTMISGHVKTGDVSSARDLFDAMPDRTVVTWTILMGWYARNSH-FDEAFKLFRQMCRSSSCTLPDHVTFTTLLPGCNDAVPQNAVGQVHAFAVKLGFDTNPFLTVSNVLLKSYCEVRRLDLACVLFEEIPEKDSVTFNTLITGYEKDGLYTESIHLFLKMRQSGHQPSDFTFSGVLKAVVGLHDFALGQQLHALSVTTGFSRDASVGNQILDFYSKHDRVLETRMLFDEMPELDFVSYNVVISSYSQADQYEASLHFFREMQCMGFDRRNFPFATMLSIAANLSSLQMGRQLHCQALLATA-----DSILHVGNSLVDMYAKCEMFEEAELIFKSLPQRTTVSWTALISGYVQKGLHGAGLKLFTKMRGSNLRADQSTFATVLKASASFASLLLGKQLHAFIIRSGNLENVFSGSGLVDMYAKCGSIKDAVQVFEEMPDRNAVSWNALISAHADNGDGEAAIGAFAKMIESGLQPDSVSILGVLTACSHCGFVEQGTEYFQAMSPIYGITPKKKHYACMLDLLGRNGRFAEAE----------------------------------KLMDEMPFEPDEIMWSSVLNACRIHKNQSLAERAAEKLFSMEKLRDAAAYVS-MSNIYAAAGEWEKVRDVKKAMR 683          

HSP 3 Score: 166.777 bits (421), Expect = 8.591e-41
Identity = 123/479 (25.68%), Postives = 225/479 (46.97%), Query Frame = -2

Query: 2119 YSKILHDCASKGSLSSAKAVHGKIIKKGFD--PDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQ-VHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQL-IERDLFSWTALI 3543
            ++ +L  C      ++   VH   +K GFD  P L + N L+ +Y +      AC + + +PE+D V++  L +G   +G   + + L+  M++ G  P+       LKA     D   G  LH   V  G   D  VG+ ++D Y+K  ++     +F  M + + VS+N +++ Y+        L+ F+ M         F  + +L     L +L  G   H   +    +    VG  L+D+Y+KC + E+   +FK + +   V+W+A+I    ++G      +LF+ MR   +  +Q TFA++   +A+    L  + +HA I + G    +   + L+ MY K  S+++  +VF+EM   N+ SWN+L+S   +    +     F KM+  G +PD  +   +L +CS+   ++ G +   A     G+         ++DL  +NG  A+AE L  ++  E D   W++++
Sbjct:  150 FTTLLPGCNDAVPQNAVGQVHAFAVKLGFDTNPFLTVSNVLLKSYCEVRRLDLACVLFEEIPEKDSVTFNTLITGYEKDGLYTESIHLFLKMRQSGHQPSDFTFSGVLKAVVGLHDFALGQQLHALSVTTGFSRDASVGNQILDFYSKHDRVLETRMLFDEMPELDFVSYNVVISSYSQADQYEASLHFFREMQCMGFDRRNFPFATMLSIAANLSSLQMGRQLHCQALLATADSILHVGNSLVDMYAKCEMFEEAELIFKSLPQRTTVSWTALISGYVQKGLHGAGLKLFTKMRGSNLRADQSTFATVLKASASFASLLLGKQLHAFIIRSGNLENVFSGSGLVDMYAKCGSIKDAVQVFEEMPDRNAVSWNALISAHADNGDGEAAIGAFAKMIESGLQPDSVSILGVLTACSHCGFVEQGTEYFQAMSPIYGITPKKKHYACMLDLLGRNGRFAEAEKLMDEMPFEPDEIMWSSVL 628          

HSP 4 Score: 142.895 bits (359), Expect = 3.678e-33
Identity = 102/340 (30.00%), Postives = 168/340 (49.41%), Query Frame = -2

Query: 2596 LFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWG---LIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCL---DEQGQIQEAAE-LFSLMRCKGVSPNQHTFASIAGVAANLGD 3591
             F+E   +   +R   ++ +L   A+  SL   + +H + +    D  LH+ NSLV+ YAK  +F  A  +   +P+R  VSWTAL SG V +G     L L+  M+   +  +     T LKA +    L  G  LH   ++ G L +VF GS LVD+YAKCG ++ A +VF  M  +N VSWNAL++ +A  GD    +  F  M ES L+    ++  VL +C++   +  G     A S +     +   +   C+LDL  + G   +  K+   +  EP+ + WS++++       Q   + AAE LFS+ + +  +     + S++ + A  G+
Sbjct:  338 FFREMQCMGFDRRNFPFATMLSIAANLSSLQMGRQLHCQALLATADSILHVGNSLVDMYAKCEMFEEAELIFKSLPQRTTVSWTALISGYVQKGLHGAGLKLFTKMRGSNLRADQSTFATVLKASASFASLLLGKQLHAFIIRSGNLENVFSGSGLVDMYAKCGSIKDAVQVFEEMPDRNAVSWNALISAHADNGDGEAAIGAFAKMIESGLQPDSVSILGVLTACSHCGFVEQGTEYFQAMSPIYGITPKKKHY--ACMLDLLGRNGRFAEAEKLMDEMPFEPDEIMWSSVLNACRIHKNQSLAERAAEKLFSMEKLRDAA----AYVSMSNIYAAAGE 671          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP285_ARATH (Pentatricopeptide repeat-containing protein At3g57430, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H81 PE=2 SV=2)

HSP 1 Score: 457.218 bits (1175), Expect = 1.452e-140
Identity = 269/835 (32.22%), Postives = 441/835 (52.81%), Query Frame = -2

Query:  922 RMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLD-VFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLV---NLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHG-FESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKML-AEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQ-----------RQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPL-IWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3372
            R PE     W  L    V      + +  Y  M   G+ P+ +     LKA +   D+  G  +H    K G  +D V V ++LV+LY KCG      +VF  + ++N VSWN+L++           L  F+ M +  +  S FTL +V+ +C+ L     L  G   H+  ++ G E + F+   L+ +Y K G    +  +       ++V W+ ++  L +  Q+ EA E    M  +GV P++ T +S+    ++L      + +HA   K+G  +    + +AL+ MY   + V +G RVF  M       WN++++G    +       +F  M  + G   +  T   ++ +C           +H  ++K GL  +  V  +L+D+Y++ G +  A  +F ++ +RDL +W  +I GY  S+  E A+   + MQ           R  + PN+ TL T L SC+ ++ L  G+ +H+ AIK   + DV V S++VDMYA CGC++ +   F  +   + ++WN+++  Y  H  G + ++  RMM+ +G++P+EVTFI+V +ACS+ G V+EG   F  +   +G+ P+  H AC++D+LGRAG+  E    +  M    N    W ++LG  R+H N ++GE AA+ L +L P +ASHY+LLANI+++ G W+    VR  M   GV+KEPGCSW+    +VH F + D  HP+  ++   L+ L E+    GYVP T  +L++V +DEK   L  H+E+LA+AF +++ +P   IR+ KNL +C DCH   K +S ++ +EI++RD   FH FK+G CSC DY
Sbjct:   60 RSPEW----WIDLLRSKVRSNLLREAVLTYVDMIVLGIKPDNYAFPALLKAVADLQDMELGKQIHAHVYKFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKG-ELNSFIINTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMPRDFNKAGAWSSLLGASRIHNNLEIGEIAAQNLIQLEPNVASHYVLLANIYSSAGLWDKATEVRRNMKEQGVRKEPGCSWIEHGDEVHKFVAGDSSHPQSEKLSGYLETLWERMRKEGYVPDTSCVLHNVEEDEKEILLCGHSEKLAIAFGILNTSPGTIIRVAKNLRVCNDCHLATKFISKIVDREIILRDVRRFHRFKNGTCSCGDY 889          

HSP 2 Score: 221.476 bits (563), Expect = 4.243e-58
Identity = 158/575 (27.48%), Postives = 280/575 (48.70%), Query Frame = -2

Query: 1873 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPD-LHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACS---LSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGC-EHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELF-SLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKM------LAEG-----FKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEA 3543
            +  +L   A    +   K +H  + K G+  D + + N+LVN Y K G FG   KV DR+ ER+ VSW +L S L +       L+ +  M  + V P+   LV+ + ACS   +   L  G  +H  G++ G  L+ F+ ++LV +Y K G++  ++ +      +++V+WN +L+         + L   + M    +   +FT+S+VL +C++L  L  G   H+  +K G  + + FVG  L+D+Y  C       +VF  + +  I  W+AMI    +    +EA  LF  +    G+  N  T A +       G     E +H  + K G + +  + N L+ MY ++  +    R+F +M   +  +WN++++G    +  +    + HKM      +++G      KP+  T  +IL SC+ L  +  G ++HA+ IKN L  +  VG++L+D+YAK G L  +  +F Q+ ++++ +W  +I  Y      ++AI     M  QGV PN  T  +  ++CS+  M++ G ++ + M    G        + +VD+    G I+EA
Sbjct:  100 FPALLKAVADLQDMELGKQIHAHVYKFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKGE-LNSFIINTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEA 673          

HSP 3 Score: 131.724 bits (330), Expect = 1.005e-29
Identity = 76/305 (24.92%), Postives = 152/305 (49.84%), Query Frame = -2

Query: 2668 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMK-----------KDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-----EPNIVAWSAMIDCLDEQGQIQEAAELFSLM 3534
            ++  C   G+ S  +A+HG ++K+G D D  + N+L++ Y++ G    A ++  +M +RD+V+W  + +G V   +  D L L   M+           +  + PN   L+T L +C+    L  G  +H   +K  +  DV VGS+LVD+YAKCG ++++ +VF  + +KNV++WN ++  Y + G+  + ++L + M    ++ ++ T  +V  +C++                                    G+ ++ L++F  ++     EP+   ++ ++D L   G+I+EA +L ++M
Sbjct:  411 VVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSH-----------------------------------SGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMM 680          

HSP 4 Score: 72.7886 bits (177), Expect = 1.565e-11
Identity = 54/201 (26.87%), Postives = 96/201 (47.76%), Query Frame = -2

Query: 3028 EKTKLEGAKRLKLYSK------ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVG--------SSLVDLYAKCGQMELAERVFLFM--DKKNVVSWNALL 3582
            E+   +GA R+ L         IL  CA+  +L+  K +H   IK     D+ + ++LV+ YAK G    + KV D++P+++V++W  +       G G + +DL   M   GV PN    ++   ACS S     G +   EG+++  ++    G        + +VDL  + G+++ A ++   M  D     +W++LL
Sbjct:  501 ERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHS-----GMV--DEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMPRDFNKAGAWSSLL 694          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: B8AB74_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_02714 PE=4 SV=1)

HSP 1 Score: 667.537 bits (1721), Expect = 0.000e+0
Identity = 349/785 (44.46%), Postives = 493/785 (62.80%), Query Frame = -2

Query: 1261 NPSLKVPLFKEKTKLE-GAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGC 3612
            +PS   P  ++  +L   A+ L+ Y+  L  CA+  +L   KA+H ++++ G  PD  L +SL+N Y K G    A  V D MP RDVV+WTA+ S +   G     L L+  M ++GV+PNG  L  ALKAC++  DL F   +H + VK+  L D +V SSLV+ Y  CG++++AER  L    ++ VSWNALLN YA  GD  K++ +F  + ES    SK+TL  VLK C  L     G   H LVIK G E D  +  CL+++YSKC  AED  +VF RI EP++V  S MI C D      EA ++F  M   GV PNQ+TF  +A VA+  GD   C  +HA I K GF     + +A++ MY+K  +VQ+    F  M   + ASWN+LLSG  +G  C+ G  IF +++ EG   +  T+  ILR C++L++++FG QVHA ++K+G + +  V   L+D+Y + G   +A L+F +L ERD+FSWT +++ YA++D  EKAI+CF  M R+   PN+ TLAT LS CS++A L +G  LHS  IK+G +  V VSS++VDMY  CG + +AE  F    + D V WN ++ GY QH  G K LE F+ M+D+G  PDE+TF+ VLSACS+ G ++EG+ YF  L++V+GI PT++H ACM+DIL +AGK  E ES I +M +T +  +W+T+LG CRMHGN ++ E AAEKLFE  P   S  ILL+NI+A   RW DV ++R+++   GVKKEPGCSW+ +NG++H+F S DGC
Sbjct:   39 HPSAPSPSPRDDERLRLRAEELRSYAAALQGCAASRALRRGKALHARLLRSGPRPDAFLHDSLLNMYCKCGRLADARSVFDGMPHRDVVAWTAMVSAITAAGDAGAALRLFAEMSEEGVVPNGFALAAALKACTVGSDLGFTPQVHAQAVKLEGLFDPYVSSSLVEAYVSCGEVDVAERALLDSPVRSDVSWNALLNEYARDGDYAKVMLVFDKLVESGDEISKYTLPTVLKCCMELGLAKSGQAVHGLVIKRGLETDRVLNNCLIEMYSKCLSAEDAYEVFARIDEPDVVHCSLMISCFDRHDMAPEAFDIFMQMSDMGVKPNQYTFVGLAIVASRTGDVNLCRSIHAHIVKSGFSRTKGVCDAIVGMYVKTGAVQDAILAFDLMQGPDIASWNTLLSGFYSGNNCEHGLRIFKELICEGVLANKYTYVGILRCCTSLMDLRFGCQVHACVLKSGFQGDYDVSKMLLDMYVQAGCFTNARLVFDRLKERDVFSWTVVMSTYAKTDEGEKAIECFRSMLRENKRPNDATLATSLSVCSDLACLGSGLQLHSYTIKSGWNSSV-VSSALVDMYVKCGNLADAEMLFDESDTHDLVEWNTIICGYAQHGHGYKALEAFQEMIDEGNVPDEITFVGVLSACSHAGLLDEGRRYFKLLSSVYGITPTLEHYACMVDILAKAGKLAEAESLINEMPLTPDASLWKTILGACRMHGNIEIAERAAEKLFESQPDDISSCILLSNIYADLKRWNDVAKLRSMLVDRGVKKEPGCSWIEINGKLHVFLSQDGC 822          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A0E0FP70_ORYNI (Uncharacterized protein OS=Oryza nivara PE=4 SV=1)

HSP 1 Score: 667.537 bits (1721), Expect = 0.000e+0
Identity = 349/785 (44.46%), Postives = 493/785 (62.80%), Query Frame = -2

Query: 1261 NPSLKVPLFKEKTKLE-GAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGC 3612
            +PS   P  ++  +L   A+ L+ Y+  L  CA+  +L   KA+H ++++ G  PD  L +SL+N Y K G    A  V D MP RDVV+WTA+ S +   G     L L+  M ++GV+PNG  L  ALKAC++  DL F   +H + VK+  L D +V SSLV+ Y  CG++++AER  L    ++ VSWNALLN YA  GD  K++ +F  + ES    SK+TL  VLK C  L     G   H LVIK G E D  +  CL+++YSKC  AED  +VF RI EP++V  S MI C D      EA ++F  M   GV PNQ+TF  +A VA+  GD   C  +HA I K GF     + +A++ MY+K  +VQ+    F  M   + ASWN+LLSG  +G  C+ G  IF +++ EG   +  T+  ILR C++L++++FG QVHA ++K+G + +  V   L+D+Y + G   +A L+F +L ERD+FSWT +++ YA++D  EKAI+CF  M R+   PN+ TLAT LS CS++A L +G  LHS  IK+G +  V VSS++VDMY  CG + +AE  F    + D V WN ++ GY QH  G K LE F+ M+D+G  PDE+TF+ VLSACS+ G ++EG+ YF  L++V+GI PT++H ACM+DIL +AGK  E ES I +M +T +  +W+T+LG CRMHGN ++ E AAEKLFE  P   S  ILL+NI+A   RW DV ++R+++   GVKKEPGCSW+ +NG++H+F S DGC
Sbjct:   39 HPSAPSPSPRDDERLRLRAEELRSYAAALQGCAASRALRRGKALHARLLRSGPRPDAFLHDSLLNMYCKCGRLADARSVFDGMPHRDVVAWTAMVSAITAAGDAGAALRLFAEMSEEGVVPNGFALAAALKACTVGSDLGFTPQVHAQAVKLEGLFDPYVSSSLVEAYVSCGEVDVAERALLDSPVRSDVSWNALLNEYARDGDYAKVMLVFDKLVESGDEISKYTLPTVLKCCMELGLAKSGQAVHGLVIKRGLETDRVLNNCLIEMYSKCLSAEDAYEVFARIDEPDVVHCSLMISCFDRHDMAPEAFDIFMQMSDMGVKPNQYTFVGLAIVASRTGDVNLCRSIHAHIVKSGFSRTKGVCDAIVGMYVKTGAVQDAILAFDLMQGPDIASWNTLLSGFYSGNNCEHGLRIFKELICEGVLANKYTYVGILRCCTSLMDLRFGCQVHACVLKSGFQGDYDVSKMLLDMYVQAGCFTNARLVFDRLKERDVFSWTVVMSTYAKTDEGEKAIECFRSMLRENKRPNDATLATSLSVCSDLACLGSGLQLHSYTIKSGWNSSV-VSSALVDMYVKCGNLADAEMLFDESDTHDLVEWNTIICGYAQHGHGYKALEAFQEMIDEGNVPDEITFVGVLSACSHAGLLDEGRRYFKLLSSVYGITPTLEHYACMVDILAKAGKLAEAESLINEMPLTPDASLWKTILGACRMHGNIEIAERAAEKLFESQPDDISSCILLSNIYADLKRWNDVAKLRSMLVDRGVKKEPGCSWIEINGKLHVFLSQDGC 822          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A0E0MYW2_ORYRU (Uncharacterized protein OS=Oryza rufipogon PE=4 SV=1)

HSP 1 Score: 667.152 bits (1720), Expect = 0.000e+0
Identity = 350/785 (44.59%), Postives = 494/785 (62.93%), Query Frame = -2

Query: 1261 NPSLKVPLFKEKTKLE-GAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGC 3612
            +PS   P  ++  +L   A+ L+ Y+  L  CA+  +L   KA+H ++++ G  PD  L +SL+N Y K G    A  V D MP RDVV+WTA+ S +   G     L L+  M ++GV+PNG  L  ALKAC++  DL F   +H + VK+  LLD +V SSLV+ Y  CG++++AER  L    ++ VSWNALLN YA  GD  K++ +F  + ES    SK+TL  VLK C  L     G   H LVIK G E D  +  CL+++YSKC  AED  +VF RI EP++V  S MI C D      EA ++F  M   GV PNQ+TF  +A VA+  GD   C  +HA I K GF     + +A++ MY+K  +VQ+    F  M   + ASWN+LLSG  +G  C+ G  IF +++ EG   +  T+  ILR C++L++++FG QVHA ++K+G + +  V   L+D+Y + G   +A L+F +L ERD+FSWT +++ YA++D  EKAI+CF  M R+   PN+ TLAT LS CS++A L +G  LHS  IK+G +  V VSS++VDMY  CG + +AE  F    + D V WN ++ GY QH  G K LE F+ M+D+G  PDE+TF+ VLSACS+ G ++EG+ YF  L++V+GI PT++H ACM+DIL +AGK  E ES I +M +T +  +W+T+LG CRMHGN ++ E AAEKLFE  P   S  ILL+NI+A   RW DV ++R+++   GVKKEPGCSW+ +NG++H+F S DGC
Sbjct:   39 HPSAPSPSPRDDERLRLRAEELRSYAAALQGCAASRALRRGKALHARLLRSGPRPDAFLHDSLLNMYCKCGRLADARSVFDGMPHRDVVAWTAMVSAITAAGDAGAALRLFAEMSEEGVVPNGFALAAALKACTVGSDLGFTPQVHAQAVKLEGLLDPYVSSSLVEAYVSCGEVDVAERALLDSPVRSDVSWNALLNEYARDGDYAKVMLVFDKLVESGDEISKYTLPTVLKCCMELGLAKSGQAVHGLVIKRGLETDRVLNNCLIEMYSKCLSAEDAYEVFARIDEPDVVHCSLMISCFDRHDMAPEAFDIFMQMSDMGVKPNQYTFVGLAIVASITGDVNLCRSIHAHIVKSGFSRTKGVCDAIVGMYVKTGAVQDAILAFDLMQGPDIASWNTLLSGFYSGNNCEHGLRIFKELICEGVLANKYTYVGILRCCTSLMDLRFGCQVHACVLKSGFQGDYDVSKMLLDMYVQAGCFTNARLVFDRLKERDVFSWTVVMSTYAKTDEGEKAIECFRSMLRENKRPNDATLATSLSVCSDLACLGSGLQLHSYTIKSGWNSSV-VSSALVDMYVKCGNLADAEMLFDESDTHDLVEWNTIICGYAQHGHGYKALEAFQEMIDEGNVPDEITFVGVLSACSHAGLLDEGRRYFKLLSSVYGITPTLEHYACMVDILAKAGKLAEAESLINEMPLTPDASLWKTILGACRMHGNIEIAERAAEKLFESQPDDISSCILLSNIYADLKRWNDVAKLRSMLVDRGVKKEPGCSWIEINGKLHVFLSQDGC 822          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A0D9YBC5_9ORYZ (Uncharacterized protein OS=Oryza glumipatula PE=4 SV=1)

HSP 1 Score: 667.152 bits (1720), Expect = 0.000e+0
Identity = 350/785 (44.59%), Postives = 494/785 (62.93%), Query Frame = -2

Query: 1261 NPSLKVPLFKEKTKLE-GAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGC 3612
            +PS   P  ++  +L   A+ L+ Y+  L  CA+  +L   KA+H ++++ G  PD  L +SL+N Y K G    A  V D MP RDVV+WTA+ S +   G     L L+  M ++GV+PNG  L  ALKAC++  DL F   +H + VK+  LLD +V SSLV+ Y  CG++++AER  L    ++ VSWNALLN YA  GD  K++ +F  + ES    SK+TL  VLK C  L     G   H LVIK G E D  +  CL+++YSKC  AED  +VF RI EP++V  S MI C D      EA ++F  M   GV PNQ+TF  +A VA+  GD   C  +HA I K GF     + +A++ MY+K  +VQ+    F  M   + ASWN+LLSG  +G  C+ G  IF +++ EG   +  T+  ILR C++L++++FG QVHA ++K+G + +  V   L+D+Y + G   +A L+F +L ERD+FSWT +++ YA++D  EKAI+CF  M R+   PN+ TLAT LS CS++A L +G  LHS  IK+G +  V VSS++VDMY  CG + +AE  F    + D V WN ++ GY QH  G K LE F+ M+D+G  PDE+TF+ VLSACS+ G ++EG+ YF  L++V+GI PT++H ACM+DIL +AGK  E ES I +M +T +  +W+T+LG CRMHGN ++ E AAEKLFE  P   S  ILL+NI+A   RW DV ++R+++   GVKKEPGCSW+ +NG++H+F S DGC
Sbjct:   39 HPSAPSPSPRDDERLRLRAEELRSYAAALQGCAASRALLRGKALHARLLRSGPRPDAFLHDSLLNMYCKCGRLADARSVFDGMPHRDVVAWTAMVSAITAAGDAGAALRLFAEMSEEGVVPNGFALAAALKACTVGSDLGFTPQVHAQAVKLEGLLDPYVSSSLVEAYVSCGEVDVAERALLDSPVRSDVSWNALLNEYARDGDYAKVMLVFDKLVESGDEISKYTLPTVLKCCMELGLAKSGQAVHGLVIKRGLETDRVLNNCLIEMYSKCLSAEDAYEVFARIDEPDVVHCSLMISCFDRHDMAPEAFDIFMQMSDMGVKPNQYTFVGLAIVASRTGDVNLCRSIHAPIVKSGFSRTKGVCDAIVGMYVKTGAVQDAILAFDLMQGPDIASWNTLLSGFYSGNNCEHGLRIFKELICEGVLANKYTYVGILRCCTSLMDLRFGCQVHACVLKSGFQGDYDVSKMLLDMYVQAGCFTNARLVFDRLKERDVFSWTVVMSTYAKTDEGEKAIECFRSMLRENKRPNDATLATSLSVCSDLACLGSGLQLHSYTIKSGWNSSV-VSSALVDMYVKCGNLADAEMLFDESDTHDLVEWNTIICGYAQHGHGYKALEAFQEMIDEGNVPDEITFVCVLSACSHAGLLDEGRMYFKLLSSVYGITPTLEHYACMVDILAKAGKLAEAESLINEMPLTPDASLWKTILGACRMHGNIEIAERAAEKLFESQPDDISSCILLSNIYADLKRWNDVAKLRSMLVDRGVKKEPGCSWIEINGKLHVFLSQDGC 822          

BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: T1MAF5_TRIUA (Uncharacterized protein OS=Triticum urartu PE=4 SV=1)

HSP 1 Score: 655.596 bits (1690), Expect = 0.000e+0
Identity = 341/783 (43.55%), Postives = 492/783 (62.84%), Query Frame = -2

Query: 1249 PLFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACS-LSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKH 3594
            P       L  A  L+ ++  L DCA + +L   + +H ++++    PD  L +SL+N Y K G    A +V D MP RDVV+WTAL S     G   + LDL+C M + G+ PNG  L + LKACS +S    F   +HG+ VK+  L D +VGSSLV  YA  G+++ AE V L + +++ +SWNALL  YA +GD  K +++F  ++E     SK+T+  +LK C  L     G   H+LV+K G E D  +  CL+++YS+C  AE+  +VF RI EP++V  SAMI      G   EA +LF  M   GV PNQ+TF  IAGVA+  GD   C   HA + K G     +++++++ MY+K+ + Q+    F  M   ++ SWN+ LSG  +G  C++G  IF +M  EGF  +  T+  +LR C++L+N+ +G QVHA ++K+GL+ +N V   L+D+YA++G    A L+F +L ERD FSWT +++GYA++D +EK ++CF  M ++   PN+ TLA  LS  S+MA L +G  LHS AIK+G +  V VS ++VDMY  CG I +AE  F    + D V+WN ++ GY QH  G K L+TFR M+D G  PD++TF+ VLSACS+ G ++EG++YF  L++V+GI PT++H ACMIDIL +AG+  E ES I  M +  +  IW T+LG CR+HGN ++ E AAE+L EL+P   S  +LL+NI+A  GRW DV R+R ++   GVKKEPGCSW+ VNGQ+H+F S DGC PK+
Sbjct:   24 PSHPNSRPLPEADELRSHAAALQDCAGRPALRCGQELHARLLRSARQPDTFLLDSLLNMYCKCGRLADARRVFDGMPHRDVVAWTALLSAYAAAGDAEEALDLFCQMNQQGLAPNGFTLASVLKACSVMSSHSEFTRQVHGQVVKLQGLDDPYVGSSLVQAYAIHGEVDAAETVLLGLPERSDMSWNALLTEYARQGDYRKAMHVFHKLSEFGDEISKYTVPTLLKCCVELGLAKSGQALHALVVKRGLETDNVLNNCLVEMYSRCLSAEEAYQVFIRIDEPDVVHCSAMISSFGRHGMAWEAFDLFVKMSDMGVKPNQYTFVGIAGVASKTGDANLCRCAHAYVVKSGLAMPKLVADSILNMYVKVGAAQDATVAFHLMHEPDTFSWNTFLSGFYSGSNCEQGLRIFKQMKCEGFSANKYTYVGVLRCCTSLMNLMYGTQVHACVLKSGLQSDNDVSRMLLDMYAQSGCFTSACLVFDRLEERDAFSWTVIMSGYAKTDEAEKVMECFRSMLQENKRPNDATLAVSLSVSSDMASLGSGLQLHSWAIKSGWNSSV-VSGALVDMYVKCGNITDAEMLFYESETCDQVAWNTLICGYSQHGHGYKALDTFRRMVDDGKRPDDITFVGVLSACSHAGLLDEGRKYFQLLSSVYGITPTMEHYACMIDILSKAGRLAEAESLINQMPLIPDSSIWRTILGACRIHGNIEIAERAAERLLELDPQDVSSSVLLSNIYADLGRWSDVTRLRNMLLDHGVKKEPGCSWIEVNGQIHVFLSQDGC-PKY 804