g.barbadense_cottongen_reftransV1_0091917, g.barbadense_cottongen_reftransV1_0091917 (contig) Gossypium barbadense
|
Overview
Alignments
The following features are aligned
Analyses
This contig is derived from or has results from the following analyses
Homology
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP307_ARATH (Pentatricopeptide repeat-containing protein At4g13650 OS=Arabidopsis thaliana GN=PCMP-H42 PE=2 SV=2) HSP 1 Score: 523.857 bits (1348), Expect = 1.215e-163 Identity = 279/875 (31.89%), Postives = 474/875 (54.17%), Query Frame = -2
Query: 922 YSKILHDCASKG-SLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3543
+S +L C + + +H +I+ +G + N L++ Y++ G A +V D + +D SW A+ SGL + + L+C M G++P + + L AC L G LHG +K+G D +V ++LV LY G + AE +F M +++ V++N L+NG + G K + LF+ M L TL++++ +C+ L G H+ K G + + LL+LY+KC E L F + N+V W+ M+ ++ + +F M+ + + PNQ+T+ SI LGD E +H+ I K F+ + + LI MY K+ + W + + + SW ++++G D+ + F +ML G + D T+ + +C+ L +K G Q+HA +G + +L+ LY++ G + ++ L F Q D +W AL++G+ QS +E+A++ F M R+G+ NNFT + + + S A ++ G+ +H++ K G + V ++++ MYA CG I +AE FL + + + VSWN ++ Y +H G + L++F M+ + P+ VT + VLSACS++G V++G YF+S+ + +G+ P +H C++D+L RAG + + FI++M + + L+W T+L C +H N ++GE AA L EL P ++ Y+LL+N++A +W+ R M GVKKEPG SW+ V +H F D HP EI+ ++L +++ GYV +LN++ ++K +F H+E+LA++F L+S+ I + KNL +C DCH ++K VS V +EI+VRDA FHHF+ G CSC+DY
Sbjct: 189 FSGVLEACRGGSVAFDVVEQIHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDGLRLKDHSSWVAMISGLSKNECEAEAIRLFCDMYVLGIMPTPYAFSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTAWDILIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAVSACAGLQALKEGQQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEESYLAFEQTEAGDNIAWNALVSGFQQSGNNEEALRVFVRMNREGIDNNNFTFGSAVKAASETANMKQGKQVHAVITKTGYDSETEVCNALISMYAKCGSISDAEKQFLEVSTKNEVSWNAIINAYSKHGFGSEALDSFDQMIHSNVRPNHVTLVGVLSACSHIGLVDKGIAYFESMNSEYGLSPKPEHYVCVVDMLTRAGLLSRAKEFIQEMPIKPDALVWRTLLSACVVHKNMEIGEFAAHHLLELEPEDSATYVLLSNLYAVSKKWDARDLTRQKMKEKGVKKEPGQSWIEVKNSIHSFYVGDQNHPLADEIHEYFQDLTKRASEIGYVQDCFSLLNELQHEQKDPIIFIHSEKLAISFGLLSLPATVPINVMKNLRVCNDCHAWIKFVSKVSNREIIVRDAYRFHHFEGGACSCKDY 1063 HSP 2 Score: 286.574 bits (732), Expect = 5.759e-79 Identity = 195/713 (27.35%), Postives = 342/713 (47.97%), Query Frame = -2
Query: 1390 SKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKAC---SLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFEL-NPGIASHYILLANIFAA 3516
+ GSL + +H +I+K G D + L L + Y +G A KV D MPER + +W + L + +V L+ M + V PN L+AC S++ D+ +H + G+ V + L+DLY++ G ++LA RVF + K+ SW A+++G + + + LF M + + + S+VL +C + +L G H LV+K G D +V L+ LY G +F + + + V ++ +I+ L + G ++A ELF M G+ P+ +T AS+ + G + +HA K GF S + AL+ +Y K ++ F E N WN +L IF +M E P+ T+ SIL++C L +++ G Q+H+ IIK + N V + LID+YAK G L A + + +D+ SWT +IAGY Q + +KA+ F M +G+ + L +S+C+ + L+ GQ +H+ A +G S D+ +++V +Y+ CG IEE+ AF + D ++WN ++ G+ Q + L F M +G++ + TF + + A S + +GK+ +T G + C +I + + G ++ E ++ T N + W ++ HG G A + ++ + + +++ L + +A
Sbjct: 97 TNGSLDEGRKLHSQILKLGLDSNGCLSEKLFDFYLFKGDLYGAFKVFDEMPERTIFTWNKMIKELASRNLIGEVFGLFVRMVSENVTPNEGTFSGVLEACRGGSVAFDVV--EQIHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDGLRLKDHSSWVAMISGLSKNECEAEAIRLFCDMYVLGIMPTPYAFSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTAWDILIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAVSACAGLQALKEGQQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEESYLAFEQTEAGDNIAWNALVSGFQQSGNNEEALRVFVRMNREGIDNNNFTFGSAVKAASETANMKQGKQVHAVITKT-GYDSETEVCNALISMYAKCGSISDAEKQFLEVS-TKNEVSWNAIINAYSKHG---FGSEALDSFDQMIHSNVRPNHVTLVGVLSA 802
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP172_ARATH (Pentatricopeptide repeat-containing protein At2g27610 OS=Arabidopsis thaliana GN=PCMP-H60 PE=2 SV=1) HSP 1 Score: 516.153 bits (1328), Expect = 5.126e-163 Identity = 286/828 (34.54%), Postives = 459/828 (55.43%), Query Frame = -2
Query: 922 ACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMX---XXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNM-AMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKS-GICSCQDY 3390
A + D+ P RD S+ +L G +G + L+ ++ + G+ + + LK + D FG LH + +K G L DV VG+SLVD Y K + +VF M ++NVV+W L++GYA ++L LF M + + FT + L GL H++V+K G + V L++LY KCG +F + + ++V W++MI G EA +F MR V ++ +FAS+ + ANL + + E +H + K+GF + + AL+ Y K ++ + R+FKE+ + S + +GK + +F +M +G +P+ T++ IL + L + +VHA ++K + ++ VGT+L+D Y K G + +A +F+ + ++D+ +W+A++AGYAQ+ +E AIK F + + G+ PN FT ++ L+ C+ A + G+ H AIK+ + VSS+++ MYA G IE AE F D VSWN M+ GY QH Q +K L+ F+ M + ++ D VTFI V +AC++ G V EG++YFD + I PT +H +CM+D+ RAG+ + I++M + IW T+L CR+H +LG AAEK+ + P ++ Y+LL+N++A G W++ +VR LM VKKEPG SW+ V + + F + D HP +IY+KL++L + GY P T ++L D+ D+ K L H+ERLA+AF LI+ + I KNL +C DCH +KL++ + ++EIVVRD+N FHHF S G+CSC D+
Sbjct: 46 AHNLFDKSPGRDRESYISLLFGFSRDGRTQEAKRLFLNIHRLGMEMDCSIFSSVLKVSATLCDELFGRQLHCQCIKFGFLDDVSVGTSLVDTYMKGSNFKDGRKVFDEMKERNVVTWTTLISGYARNSMNDEVLTLFMRMQNEGTQPNSFTFAAALGVLAEEGVGGRGLQVHTVVVKNGLDKTIPVSNSLINLYLKCGNVRKARILFDKTEVKSVVTWNSMISGYAANGLDLEALGMFYSMRLNYVRLSESSFASVIKLCANLKELRFTEQLHCSVVKYGFLFDQNIRTALMVAYSKCTAMLDALRLFKEIGCVGNVVSWTAMISGFLQNDGK--EEAVDLFSEMKRKGVRPNEFTYSVILTA----LPVISPSEVHAQVVKTNYERSSTVGTALLDAYVKLGKVEEAAKVFSGIDDKDIVAWSAMLAGYAQTGETEAAIKMFGELTKGGIKPNEFTFSSILNVCAATNASMGQGKQFHGFAIKSRLDSSLCVSSALLTMYAKKGNIESAEEVFKRQREKDLVSWNSMISGYAQHGQAMKALDVFKEMKKRKVKMDGVTFIGVFAACTHAGLVEEGEKYFDIMVRDCKIAPTKEHNSCMVDLYSRAGQLEKAMKVIENMPNPAGSTIWRTILAACRVHKKTELGRLAAEKIIAMKPEDSAAYVLLSNMYAESGDWQERAKVRKLMNERNVKKEPGYSWIEVKNKTYSFLAGDRSHPLKDQIYMKLEDLSTRLKDLGYEPDTSYVLQDIDDEHKEAVLAQHSERLAIAFGLIATPKGSPLLIIKNLRVCGDCHLVIKLIAKIEEREIVVRDSNRFHHFSSDGVCSCGDF 867 HSP 2 Score: 254.218 bits (648), Expect = 5.261e-69 Identity = 182/620 (29.35%), Postives = 310/620 (50.00%), Query Frame = -2
Query: 1708 LYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNL-LNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSS-IVDMYANCGCIEEAEAAFLGMVS-TDTVSWNMMLFGYLQHRQGLKVLETFRMMLDK--GLEPDEVTFIAVLS 3546
++S +L A+ + +H + IK GF D+ + SLV+ Y K F KV D M ER+VV+WT L SG +VL L+ M+ +G PN AL + G +H VK G+ + V +SL++LY KCG + A +F + K+VV+WN++++GYA G + L +F M + +R S+ + ++V+K C L L + H V+K G D+ + L+ YSKC D L++FK I N+V+W+AMI + +EA +LFS M+ KGV PN+ T++ I + VHA + K +E + AL+ Y+K+ V+ +VF + + + L +Q G+T + +F ++ G KP+ TF+SIL C+ ++ G Q H IK+ L + V ++L+ +YAK G + AE +F + E+DL SW ++I+GYAQ + KA+ F M+++ V + T ++C++ ++E G+ + ++ +S +VD+Y+ G +E+A M + + W +L H++ E R+ +K ++P++ +LS
Sbjct: 95 IFSSVLKVSATLCDELFGRQLHCQCIKFGFLDDVSVGTSLVDTYMKGSNFKDGRKVFDEMKERNVVTWTTLISGYARNSMNDEVLTLFMRMQNEGTQPNSFTFAAALGVLAEEGVGGRGLQVHTVVVKNGLDKTIPVSNSLINLYLKCGNVRKARILFDKTEVKSVVTWNSMISGYAANGLDLEALGMFYSMRLNYVRLSESSFASVIKLCANLKELRFTEQLHCSVVKYGFLFDQNIRTALMVAYSKCTAMLDALRLFKEIGCVGNVVSWTAMISGFLQNDGKEEAVDLFSEMKRKGVRPNEFTYSVILTALPVISP----SEVHAQVVKTNYERSSTVGTALLDAYVKLGKVEEAAKVFSGIDDKDIVAWSAMLAGYAQTGET-EAAIKMFGELTKGGIKPNEFTFSSILNVCAATNASMGQGKQFHGFAIKSRLDSSLCVSSALLTMYAKKGNIESAEEVFKRQREKDLVSWNSMISGYAQHGQAMKALDVFKEMKKRKVKMDGVTFIGVFAACTHAGLVEEGEKYFDIMVRDCKIAPTKEHNSCMVDLYSRAGQLEKAMKVIENMPNPAGSTIWRTILAACRVHKK----TELGRLAAEKIIAMKPEDSAAYVLLS 705 HSP 3 Score: 70.4774 bits (171), Expect = 8.832e-11 Identity = 63/288 (21.88%), Postives = 125/288 (43.40%), Query Frame = -2
Query: 1480 FTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLY----AKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGND 2331
FT+ + +L N K +++A+ G + + +Y + L +A LF + RD S+ +L+ G+++ +++A + F ++ R G+ + ++ L + + G+ LH IK G DV V +S+VD Y ++ F M + V+W ++ GY ++ +VL F M ++G +P+ TF A L + G G + + G+ TI +I++ + G + +V S + W +++ +G D
Sbjct: 3 FTTTIWRPPSLENFKPKFRIYAN------------GVAQVRIYCFGTVSSSRLYNAHNLFDKSPGRDRESYISLLFGFSRDGRTQEAKRLFLNIHRLGMEMDCSIFSSVLKVSATLCDELFGRQLHCQCIKFGFLDDVSVGTSLVDTYMKGSNFKDGRKVFDEMKERNVVTWTTLISGYARNSMNDEVLTLFMRMQNEGTQPNSFTFAAALGVLAEEGVGGRGLQVHTVVVKN-GLDKTIPVSNSLINLYLKCGNVRKARILFDKTEVKS-VVTWNSMISGYAANGLD 276
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP348_ARATH (Pentatricopeptide repeat-containing protein At4g33990 OS=Arabidopsis thaliana GN=EMB2758 PE=3 SV=2) HSP 1 Score: 473.396 bits (1217), Expect = 1.756e-147 Identity = 263/757 (34.74%), Postives = 423/757 (55.88%), Query Frame = -2
Query: 922 LHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQ-GMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNG-LKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQG-VTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3183
LH V + +V + + LV+LY G + LA F + ++V +WN +++GY G++ +++ F M S L T +VLK+C +++ G H L +K G D +V L+ LYS+ + +F + ++ +W+AMI + G +EA L + +R + + T S+ GD +H+ KHG ESE+ +SN LI +Y + +++ +VF M + SWNS++ + + R S+F +M +PD T S+ S L +I+ V ++ G ++ +G +++ +YAK G + A +F L D+ SW +I+GYAQ+ + +AI+ +N M+ +G + N T + L +CS L G LH +K G DVFV +S+ DMY CG +E+A + F + ++V WN ++ + H G K + F+ MLD+G++PD +TF+ +LSACS+ G V+EG+ F+ + +GI P++KH CM+D+ GRAG+ FIK M + + IW +L CR+HGN LG+ A+E LFE+ P +++LL+N++A+ G+WE V +R++ G++K PG S + V+ +V +F + + HP + E+Y +L L K + GYVP +L DV DDEK L H+ERLA+AFALI+ TIRIFKNL +C DCH K +S + ++EI+VRD+N FHHFK+G+CSC DY
Sbjct: 73 LHARLVVSKQIQNVCISAKLVNLYCYLGNVALARHTFDHIQNRDVYAWNLMISGYGRAGNSSEVIRCFSLFMLSSGLTPDYRTFPSVLKACRTVID---GNKIHCLALKFGFMWDVYVAASLIHLYSRYKAVGNARILFDEMPVRDMGSWNAMISGYCQSGNAKEALTLSNGLR----AMDSVTVVSLLSACTEAGDFNRGVTIHSYSIKHGLESELFVSNKLIDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDCLTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALRQGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHGFHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHSGLVDEGQWCFEMMQTDYGITPSLKHYGCMVDMYGRAGQLETALKFIKSMSLQPDASIWGALLSACRVHGNVDLGKIASEHLFEVEPEHVGYHVLLSNMYASAGKWEGVDEIRSIAHGKGLRKTPGWSSMEVDNKVEVFYTGNQTHPMYEEMYRELTALQAKLKMIGYVPDHRFVLQDVEDDEKEHILMSHSERLAIAFALIATPAKTTIRIFKNLRVCGDCHSVTKFISKITEREIIVRDSNRFHHFKNGVCSCGDY 822 HSP 2 Score: 239.965 bits (611), Expect = 2.214e-64 Identity = 187/653 (28.64%), Postives = 309/653 (47.32%), Query Frame = -2
Query: 1564 SLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYC-SMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHG-FESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEG-FKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQL-LHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPT-IKHCACMIDILGRAGKFNEVE 3507
+L SAK +H +++ ++ + LVN Y G A D + RDV +W + SG G +V+ + M G+ P+ + LKAC +D G +H +K G + DV+V +SL+ LY++ + A +F M +++ SWNA+++GY G+A + L L G+ + T+ ++L +CT + G+ HS IK G E + FV L+DLY++ G D KVF R+ ++++W+++I + Q A LF MR + P+ T S+A + + LGD C V + G F +I + NA++ MY K+ V + VF + + + SWN+++SG +++ M EG + T+ S+L +CS ++ G ++H ++KNGL + V TSL D+Y K G L DA LF Q+ + W LIA + EKA+ F M +GV P++ T T LS+CS+ +++ GQ M G + + +VDMY G +E A LK +++ L+PD + A+LSAC G V+ GK + L F + P + + + ++ AGK+ V+
Sbjct: 66 NLQSAKCLHARLVVSKQIQNVCISAKLVNLYCYLGNVALARHTFDHIQNRDVYAWNLMISGYGRAGNSSEVIRCFSLFMLSSGLTPDYRTFPSVLKACRTVID---GNKIHCLALKFGFMWDVYVAASLIHLYSRYKAVGNARILFDEMPVRDMGSWNAMISGYCQSGNAKEALTLSNGLRAMD----SVTVVSLLSACTEAGDFNRGVTIHSYSIKHGLESELFVSNKLIDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDCLTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALRQGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHGFHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHSGLVDEGQWCFEMMQTDYGITPSLKHYGCMVDMYGRAGQLETA----------------------------LKFIKSM------SLQPDASIWGALLSACRVHGNVDLGKIASEHL---FEVEPEHVGYHVLLSNMYASAGKWEGVD 674 HSP 3 Score: 173.326 bits (438), Expect = 7.960e-43 Identity = 137/513 (26.71%), Postives = 245/513 (47.76%), Query Frame = -2
Query: 1390 YLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSL-MRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKP-DICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNS-GDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKG-LEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVES-FIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFE-LNPGIASHYILLANIFAA 2910
Y NL H+ ++ + + + L++LY G F IQ ++ AW+ MI G E FSL M G++P+ TF S+ + D +H K GF ++ ++ +LI +Y + ++V N +F EM + SWN+++SG C G + L+ G + D T S+L +C+ + G +H++ IK+GL+ V LIDLYA+ G L D + +F ++ RDL SW ++I Y ++ +AI F M+ + P+ TL + S S + + + + ++ G D+ + +++V MYA G ++ A A F + +TD +SWN ++ GY Q+ + +E + +M ++G + ++ T+++VL ACS G + +G + L G+ + + D+ G+ G+ + S F + +V S P W T++ HG+ GE A E L+ G+ +I + +A
Sbjct: 63 YCTNLQSAKCLHARLVVSKQIQNVCISAKLVNLYCYLGNVALARHTFDHIQNRDVYAWNLMISGYGRAGNSSEVIRCFSLFMLSSGLTPDYRTFPSVLKACRTVID---GNKIHCLALKFGFMWDVYVAASLIHLYSRYKAVGNARILFDEMPVRDMGSWNAMISGY-----CQSGNAKEALTLSNGLRAMDSVTVVSLLSACTEAGDFNRGVTIHSYSIKHGLESELFVSNKLIDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDCLTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALRQGMKLHGRLLKN-GLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVP--WNTLIACHGFHGH---GEKAVMLFKEMLDEGVKPDHITFVTLLSA 561 HSP 4 Score: 149.443 bits (376), Expect = 3.041e-35 Identity = 92/314 (29.30%), Postives = 157/314 (50.00%), Query Frame = -2
Query: 2599 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLL-DVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTES-ELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLG 3534
+L C G + +H IK G + +L + N L++ YA+ G KV DRM RD++SW ++ + L+ M+ + P+ L++ S D+ + G ++ G L D+ +G+++V +YAK G ++ A VF ++ +V+SWN +++GYA G A + + ++ M E E+ ++ T +VL +C+ L G+ H ++K G D FV L D+Y KCG ED L +F +I N V W+ +I C G ++A LF M +GV P+ TF ++ ++ G
Sbjct: 253 LLSACTEAGDFNRGVTIHSYSIKHGLESELFVSNKLIDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDCLTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALRQGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHGFHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHSG 566 HSP 5 Score: 132.88 bits (333), Expect = 3.924e-30 Identity = 83/279 (29.75%), Postives = 144/279 (51.61%), Query Frame = -2
Query: 2698 GSLSSAKAVHGKIIKKG-FDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDG-VLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTY--LVN---LTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCLDEQGQI 3510
G + + ++V G ++KG F D+ + N++V YAK G+ A V + +P DV+SW + SG G+ + +++Y M+++G + N V+ L ACS + L G LHG +K G+ LDVFV +SL D+Y KCG++E A +F + + N V WN L+ + G K + LF+ M + ++ T +L +C++ LV+ + ++ I +H C++D+Y + G E LK K + +P+ W A++ G +
Sbjct: 362 GDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALRQGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHGFHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHSGLVDEGQWCFEMMQTDYGITPSLKHYG----CMVDMYGRAGQLETALKFIKSMSLQPDASIWGALLSACRVHGNV 636 HSP 6 Score: 70.0922 bits (170), Expect = 1.128e-10 Identity = 61/218 (27.98%), Postives = 96/218 (44.04%), Query Frame = -2
Query: 2917 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSL------VDLYAKCGQMELAERVFLFMD-KKNVVSWNALLNGYALKG--DAGKILNLFQGMTESELRCSKFTLSNVLKS 3543
+ +L C+ G+L +HG+++K G D+ + SL + Y K G A + ++P + V W L + G+G + L+ M +GV P+ VT L ACS S G + G+ + D + SL VD+Y + GQ+E A + M + + W ALL+ + G D GKI + E E LSN+ S
Sbjct: 454 WVSVLPACSQAGALRQGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHGFHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHS-----GLVDEGQWCFEMMQTDYGITPSLKHYGCMVDMYGRAGQLETALKFIKSMSLQPDASIWGALLSACRVHGNVDLGKIASEHLFEVEPEHVGYHVLLSNMYAS 666
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP347_ARATH (Pentatricopeptide repeat-containing protein At4g33170 OS=Arabidopsis thaliana GN=PCMP-H53 PE=3 SV=1) HSP 1 Score: 476.093 bits (1224), Expect = 1.653e-146 Identity = 281/875 (32.11%), Postives = 461/875 (52.69%), Query Frame = -2
Query: 922 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELF-SLMRCKGVSPNQHTFASIAGVAANLGDRL-YCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3540
S +L C G + ++++ HG K G D D + +LVN Y K G + + MP RDVV W + + G+ + +DL + G+ PN ++L L + G+ G + G+ + ++ N L+ Y G +L F M ES++ C + T +L + + +L G H + +K G + V L+++Y K VF + E ++++W+++I + + G EA LF L+RC G+ P+Q+T S+ A++L + L + VH K S+ +S ALI Y + R ++ +F+ + A + +Q+ + +F M +G + D T ++ ++C L I G QVHA+ IK+G + V + ++D+Y K G ++ A+ F + D +WT +I+G ++ E+A F+ M+ GV P+ FT+AT + S + LE G+ +H+ A+K + D FV +S+VDMYA CG I++A F + + +WN ML G QH +G + L+ F+ M G++PD+VTFI VLSACS+ G V+E ++ S+ +GI P I+H +C+ D LGRAG + E+ I+ M + ++ ++ T+L CR+ G+ + G+ A KL EL P +S Y+LL+N++AA +W++++ R +M VKK+PG SW+ V ++HIF D + + IY K+K+++ GYVP+TD L DV ++EK L++H+E+LA+AF L+S P IR+ KNL +C DCH+ MK ++ V +EIV+RDAN FH FK GICSC DY
Sbjct: 149 SPMLKLCLHSGYVWASESFHGYACKIGLDGDEFVAGALVNIYLKFGKVKEGKVLFEEMPYRDVVLWNLMLKAYLEMGFKEEAIDLSSAFHSSGLNPN-----------EITLRLL--ARISGDDSDAGQVKSFANGNDASSV-------------------SEIIFRNKGLSEYLHSGQYSALLKCFADMVESDVECDQVTFILMLATAVKVDSLALGQQVHCMALKLGLDLMLTVSNSLINMYCKLRKFGFARTVFDNMSERDLISWNSVIAGIAQNGLEVEAVCLFMQLLRC-GLKPDQYTMTSVLKAASSLPEGLSLSKQVHVHAIKINNVSDSFVSTALIDAYSRNRCMKEAEILFERHNFDLVAWNAMMAGYTQSHDG-HKTLKLFALMHKQGERSDDFTLATVFKTCGFLFAINQGKQVHAYAIKSGYDLDLWVSSGILDMYVKCGDMSAAQFAFDSIPVPDDVAWTTMISGCIENGEEERAFHVFSQMRLMGVLPDEFTIATLAKASSCLTALEQGRQIHANALKLNCTNDPFVGTSLVDMYAKCGSIDDAYCLFKRIEMMNITAWNAMLVGLAQHGEGKETLQLFKQMKSLGIKPDKVTFIGVLSACSHSGLVSEAYKHMRSMHGDYGIKPEIEHYSCLADALGRAGLVKQAENLIESMSMEASASMYRTLLAACRVQGDTETGKRVATKLLELEPLDSSAYVLLSNMYAAASKWDEMKLARTMMKGHKVKKDPGFSWIEVKNKIHIFVVDDRSNRQTELIYRKVKDMIRDIKQEGYVPETDFTLVDVEEEEKERALYYHSEKLAVAFGLLSTPPSTPIRVIKNLRVCGDCHNAMKYIAKVYNREIVLRDANRFHRFKDGICSCGDY 989 HSP 2 Score: 234.572 bits (597), Expect = 4.446e-62 Identity = 193/697 (27.69%), Postives = 329/697 (47.20%), Query Frame = -2
Query: 1477 LHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGL------VNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLN-IKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIER---DLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKE-YFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDK 3531
L + + L K H +I+ +P+ L N+L++ Y+K G YA +V D+MP+RD+VSW ++ + V E L L+ +++D V + L LK C S ++ HG K+G+ D FV +LV++Y K G+++ + +F M ++VV WN +L Y G + ++L S L ++ TL L+ G + + +K+ ++ + ++ GL+E + +SA++ C F+ M V +Q TF + A + + VH K G + + +SN+LI MY K+R VF MS + S + + +QNG + +F ++L G KPD T TS+L++ S+L + QVH H IK ++ V T+LID Y++N + +AE+LF ER DL +W A++AGY QS K +K F M +QG ++FTLAT +C + + G+ +H+ AIK+G D++VSS I+DMY CG + A+ AF + D V+W M+ G +++ + + F M G+ PDE T + A S + + +G++ + ++L P + ++D+ + G ++ K +++ N W +L HG K
Sbjct: 46 LRNAITSSDLMLGKCTHARILTFEENPERFLINNLISMYSKCGSLTYARRVFDKMPDRDLVSWNSILAAYAQSSECVVENIQQAFL-LFRILRQDVVYTSRMTLSPMLKLCLHSGYVWASESFHGYACKIGLDGDEFVAGALVNIYLKFGKVKEGKVLFEEMPYRDVVLWNLMLKAYLEMGFKEEAIDLSSAFHSSGLNPNEITLR--------LLARISGDDSDAGQVKSFANGNDASSVSEI-IFRNKGLSEYL----------HSGQYSALLKC-------------FADMVESDVECDQVTFILMLATAVKVDSLALGQQVHCMALKLGLDLMLTVSNSLINMYCKLRKFGFARTVFDNMSERDLISWNSVIAGIAQNGLEVE-AVCLFMQLLRCGLKPDQYTMTSVLKAASSLPEGLSLSKQVHVHAIKINNVSDSFVSTALIDAYSRNRCMKEAEILF----ERHNFDLVAWNAMMAGYTQSHDGHKTLKLFALMHKQGERSDDFTLATVFKTCGFLFAINQGKQVHAYAIKSGYDLDLWVSSGILDMYVKCGDMSAAQFAFDSIPVPDDVAWTTMISGCIENGEEERAFHVFSQMRLMGVLPDEFTIATLAKASSCLTALEQGRQIHANALKLNCTNDPFVG--TSLVDMYAKCGSIDDAYCLFKRIEMM-NITAWNAMLVGLAQHGEGK 701
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP373_ARATH (Putative pentatricopeptide repeat-containing protein At5g09950 OS=Arabidopsis thaliana GN=PCMP-H35 PE=3 SV=1) HSP 1 Score: 473.396 bits (1217), Expect = 1.964e-145 Identity = 309/892 (34.64%), Postives = 480/892 (53.81%), Query Frame = -2
Query: 922 YSKILHDCASKGSLSS--AKAVHGKIIKKGFDPDLHLWNSLVNAYAK-RGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHC---LVTALKACSLS-LDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKG---DAGKILNLFQGMTESELRCSKFTLSNVLK-SCTYLVNLTWGLIAHSLVIKTG-CEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIER-DLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMM-LDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTC-RMHGND-KLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISI-NPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3543
+ +L C GS+ + +HG + K + D + N L++ Y K G GYA + ++ VSW ++ S G ++ SM+ DG P + LVT ACSL+ D+ + K G+L D+FVGS LV +AK G + A +VF M+ +N V+ N L+ G + +A K+ M + LS+ + S V L G H VI TG + +G L+++Y+KCG D +VF + + + V+W++MI LD+ G EA E + MR + P T S A+L + +H K G + + +SNAL+ +Y + + ++F M + S + + + +++ ++ F G K + TF+S+L + S+L + G Q+H +KN + D +LI Y K G + E +F+++ ER D +W ++I+GY ++ KA+ M + G ++F AT LS+ +++A LE G +H+ +++A DV V S++VDMY+ CG ++ A F M ++ SWN M+ GY +H QG + L+ F M LD PD VTF+ VLSACS+ G + EG ++F+S+++ +G+ P I+H +CM D+LGRAG+ +++E FI+ M + N LIW TVLG C R +G +LG+ AAE LF+L P A +Y+LL N++AA GRWED+ + R M VKKE G SWV + VH+F + D HP IY KLKEL K AGYVP+T L D+ + K E L +H+E+LA+AF L + + IRI KNL +C DCH K +S + ++I++RD+N FHHF+ G CSC D+
Sbjct: 105 FVSVLRACQEIGSVGILFGRQIHGLMFKLSYAVDAVVSNVLISMYWKCIGSVGYALCAFGDIEVKNSVSWNSIISVYSQAGDQRSAFRIFSSMQYDGSRPTEYTFGSLVTT--ACSLTEPDVRLLEQIMCTIQKSGLLTDLFVGSGLVSAFAKSGSLSYARKVFNQMETRNAVTLNGLMVGLVRQKWGEEATKLFMDMNSMIDVSPESYVILLSSFPEYSLAEEVGLKKGREVHGHVITTGLVDFMVGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLDQNGCFIEAVERYKSMRRHDILPGSFTLISSLSSCASLKWAKLGQQIHGESLKLGIDLNVSVSNALMTLYAETGYLNECRKIFSSMPEHDQVSWNSIIGALARSERSLPEAVVCFLNAQRAGQKLNRITFSSVLSAVSSLSFGELGKQIHGLALKNNIADEATTENALIACYGKCGEMDGCEKIFSRMAERRDNVTWNSMISGYIHNELLAKALDLVWFMLQTGQRLDSFMYATVLSAFASVATLERGMEVHACSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPVRNSYSWNSMISGYARHGQGEEALKLFETMKLDGQTPPDHVTFVGVLSACSHAGLLEEGFKHFESMSDSYGLAPRIEHFSCMADVLGRAGELDKLEDFIEKMPMKPNVLIWRTVLGACCRANGRKAELGKKAAEMLFQLEPENAVNYVLLGNMYAAGGRWEDLVKARKKMKDADVKKEAGYSWVTMKDGVHMFVAGDKSHPDADVIYKKLKELNRKMRDAGYVPQTGFALYDLEQENKEEILSYHSEKLAVAFVLAAQRSSTLPIRIMKNLRVCGDCHSAFKYISKIEGRQIILRDSNRFHHFQDGACSCSDF 994 HSP 2 Score: 240.736 bits (613), Expect = 5.697e-64 Identity = 198/709 (27.93%), Postives = 333/709 (46.97%), Query Frame = -2
Query: 1444 GSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSL--SLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKC-GQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSL--VIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCK-GVSPNQHTFASIAGVAANLGDRLYCEG---VHACIFKHGFESEII-LSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCS-EKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVS-TDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHC--------ACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFE 3510
G +A+ H ++ K D D++L N+L+NAY + G A KV D MP R+ VSW + SG G + L M K+G+ N + V+ L+AC S+ + FG +HG K+ +D V + L+ +Y KC G + A F ++ KN VSWN++++ Y+ GD +F M R +++T +++ + L L+ + + K+G D FVG L+ ++K G KVF +++ N V + ++ L Q +EA +LF M VSP + + +L + + + VH + G ++ + N L+ MY K S+ + RVF M+ +S SWNS+++G + M P T S L SC++L K G Q+H +K G+ N V +L+ LYA+ G+L + +F+ + E D SW ++I A+S+ S +A+ CF + QR G N T ++ LS+ S+++ E G+ +H +A+K + + ++++ Y CG ++ E F M D V+WN M+ GY+ + K L+ ML G D + VLSA + + + G E +++ C + ++D+ + G+ + F M V N W +++ HG GE A KLFE
Sbjct: 15 GHRGAARFFHSRLYKNRLDKDVYLCNNLINAYLETGDSVSARKVFDEMPLRNCVSWACIVSGYSRNGEHKEALVFLRDMVKEGIFSNQYAFVSVLRACQEIGSVGILFGRQIHGLMFKLSYAVDAVVSNVLISMYWKCIGSVGYALCAFGDIEVKNSVSWNSIISVYSQAGDQRSAFRIFSSMQYDGSRPTEYTFGSLVTTACSLTEPDVRLLEQIMCTIQKSGLLTDLFVGSGLVSAFAKSGSLSYARKVFNQMETRNAVTLNGLMVGLVRQKWGEEATKLFMDMNSMIDVSPESYVILLSSFPEYSLAEEVGLKKGREVHGHVITTGLVDFMVGIGNGLVNMYAKCGSIADARRVFYFMTDKDSVSWNSMITGLDQNGCFIEAVERYKSMRRHDILPGSFTLISSLSSCASLKWAKLGQQIHGESLKLGIDLNVSVSNALMTLYAETGYLNECRKIFSSMPEHDQVSWNSIIGALARSERSLPEAVVCFLNAQRAGQKLNRITFSSVLSAVSSLSFGELGKQIHGLALKNNIADEATTENALIACYGKCGEMDGCEKIFSRMAERRDNVTWNSMISGYIHNELLAKALDLVWFMLQTGQRLDSFMYATVLSAFASVATLERGMEVH---------ACSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPV-RNSYSWNSMISGYARHGQ---GEEAL-KLFE 709 HSP 3 Score: 71.633 bits (174), Expect = 4.270e-11 Identity = 54/187 (28.88%), Postives = 89/187 (47.59%), Query Frame = -2
Query: 3028 LEGAKRLK--LYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGH-CLVTALKACSLSLDLFFGTLLHGEGV--KVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMD-KKNVVSWNALL 3570
L+ +RL +Y+ +L AS +L VH ++ + D+ + ++LV+ Y+K G YA + + MP R+ SW ++ SG G G + L L+ +MK DG P H V L ACS + L H E + G+ + S + D+ + G+++ E M K NV+ W +L
Sbjct: 611 LQTGQRLDSFMYATVLSAFASVATLERGMEVHACSVRACLESDVVVGSALVDMYSKCGRLDYALRFFNTMPVRNSYSWNSMISGYARHGQGEEALKLFETMKLDGQTPPDHVTFVGVLSACSHA-GLLEEGFKHFESMSDSYGLAPRIEHFSCMADVLGRAGELDKLEDFIEKMPMKPNVLIWRTVL 796
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP296_ARATH (Pentatricopeptide repeat-containing protein At3g63370, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H83 PE=2 SV=2) HSP 1 Score: 471.855 bits (1213), Expect = 3.273e-145 Identity = 278/886 (31.38%), Postives = 472/886 (53.27%), Query Frame = -2
Query: 925 EGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIK--KGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFL-FMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGC-CLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEK-SILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQD 3567
E ++ ++ +L C + ++S + +H +I K F+ D L LV Y K G A KV D MP+R +W + V+ G L LY +M+ +GV LKAC+ D+ G+ LH VK+G F+ ++LV +YAK + A R+F F +K + V WN++L+ Y+ G + + L LF+ M + + +T+ + L +C G H+ V+K+ E C L+ +Y++CG ++ +++ ++V W+++I + +EA E FS M G ++ + SI + L + L +HA + KHG++S + + N LI MY K R F M + SW ++++G +F + + + D SILR+ S L ++ ++H HI++ GL D ++ L+D+Y K + A +F + +D+ SWT++I+ A + +A++ F M G++ ++ L LS+ ++++ L G+ +H ++ G + ++ ++VDMYA CG ++ A+A F + + + M+ Y H G +E F M + + PD ++F+A+L ACS+ G ++EG+ + + + + + P +H C++D+LGRA E F+K MK +W +L CR H ++GE AA++L EL P + +L++N+FA +GRW DV +VRA M G++K PGCSW+ ++G+VH F + D HP+ EIY KL E+ K GYV T +L++V + EK++ L H+ER+A+A+ L+ +RI KNL +C DCH F KLVS + +++IV+RDAN FHHF+SG+CSC D
Sbjct: 75 ENNSPVEAFAYVLELCGKRRAVSQGRQLHSRIFKTFPSFELDF-LAGKLVFMYGKCGSLDDAEKVFDEMPDRTAFAWNTMIGAYVSNGEPASALALYWNMRVEGVPLGLSSFPALLKACAKLRDIRSGSELHSLLVKLGYHSTGFIVNALVSMYAKNDDLSAARRLFDGFQEKGDAVLWNSILSSYSTSGKSLETLELFREMHMTGPAPNSYTIVSALTACDGFSYAKLGKEIHASVLKSSTHSSELYVCNALIAMYTRCGKMPQAERILRQMNNADVVTWNSLIKGYVQNLMYKEALEFFSDMIAAGHKSDEVSMTSIIAASGRLSNLLAGMELHAYVIKHGWDSNLQVGNTLIDMYSKCNLTCYMGRAFLRMHDKDLISWTTVIAGYAQNDCHVEALELFRDVAKKRMEIDEMILGSILRASSVLKSMLIVKEIHCHILRKGLLDT-VIQNELVDVYGKCRNMGYATRVFESIKGKDVVSWTSMISSSALNGNESEAVELFRRMVETGLSADSVALLCILSAAASLSALNKGREIHCYLLRKGFCLEGSIAVAVVDMYACCGDLQSAKAVFDRIERKGLLQYTSMINAYGMHGCGKAAVELFDKMRHENVSPDHISFLALLYACSHAGLLDEGRGFLKIMEHEYELEPWPEHYVCLVDMLGRANCVVEAFEFVKMMKTEPTAEVWCALLAACRSHSEKEIGEIAAQRLLELEPKNPGNLVLVSNVFAEQGRWNDVEKVRAKMKASGMEKHPGCSWIEMDGKVHKFTARDKSHPESKEIYEKLSEVTRKLEREVGYVADTKFVLHNVDEGEKVQMLHGHSERIAIAYGLLRTPDRACLRITKNLRVCRDCHTFCKLVSKLFRRDIVMRDANRFHHFESGLCSCGD 958
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP272_ARATH (Pentatricopeptide repeat-containing protein At3g49170, chloroplastic OS=Arabidopsis thaliana GN=EMB2261 PE=2 SV=1) HSP 1 Score: 465.692 bits (1197), Expect = 2.857e-144 Identity = 270/806 (33.50%), Postives = 445/806 (55.21%), Query Frame = -2
Query: 922 LDLYCSMKKDGVLP-NGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMD---KKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTG-CEHDEFVGCCLLDLYSKCGLA-EDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIR---SVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEG-FKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQ----LFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3294
LDL M +DG+ P + + LK+C + D G L+H ++ + D + +SL+ LY+K G AE VF M K++VVSW+A++ Y G + +F E L + + + V+++C+ + G + ++KTG E D VGC L+D++ K + E+ KVF ++ E N+V W+ MI + G +EA F M G ++ T +S+ A L + + +H+ + G ++ S L+ MY K SV + +VF M + S + +N ++F +M+ +G +P+ TF+S ++C NL + + G QV K GL N+ V S+I ++ K+ + DA+ F L E++L S+ + G ++ E+A K + + + + + FT A+ LS +N+ + G+ +HS +K G S + V ++++ MY+ CG I+ A F M + + +SW M+ G+ +H ++VLETF M+++G++P+EVT++A+LSACS++G V+EG +F+S+ I P ++H ACM+D+L RAG + FI M ++ L+W T LG CR+H N +LG+ AA K+ EL+P + YI L+NI+A G+WE+ +R M + KE GCSW+ V ++H F D HP +IY +L L+ + GYVP TD +L+ + ++ + L+ H+E++A+AF LIS + + +R+FKNL +C DCH+ MK +S V +EIV+RD N FHHFK G CSC DY
Sbjct: 49 LDL---MARDGIRPMDSVTFSSLLKSCIRARDFRLGKLVHARLIEFDIEPDSVLYNSLISLYSKSGDSAKAEDVFETMRRFGKRDVVSWSAMMACYGNNGRELDAIKVFVEFLELGLVPNDYCYTAVIRACSNSDFVGVGRVTLGFLMKTGHFESDVCVGCSLIDMFVKGENSFENAYKVFDKMSELNVVTWTLMITRCMQMGFPREAIRFFLDMVLSGFESDKFTLSSVFSACAELENLSLGKQLHSWAIRSGLVDDVECS--LVDMYAKCSADGSVDDCRKVFDRMEDHSVMSWTALITGYMKNCNLATEAINLFSEMITQGHVEPNHFTFSSAFKACGNLSDPRVGKQVLGQAFKRGLASNSSVANSVISMFVKSDRMEDAQRAFESLSEKNLVSYNTFLDGTCRNLNFEQAFKLLSEITERELGVSAFTFASLLSGVANVGSIRKGEQIHSQVVKLGLSCNQPVCNALISMYSKCGSIDTASRVFNFMENRNVISWTSMITGFAKHGFAIRVLETFNQMIEEGVKPNEVTYVAILSACSHVGLVSEGWRHFNSMYEDHKIKPKMEHYACMVDLLCRAGLLTDAFEFINTMPFQADVLVWRTFLGACRVHSNTELGKLAARKILELDPNEPAAYIQLSNIYACAGKWEESTEMRRKMKERNLVKEGGCSWIEVGDKIHKFYVGDTAHPNAHQIYDELDRLITEIKRCGYVPDTDLVLHKLEEENDEAEKERLLYQHSEKIAVAFGLISTSKSRPVRVFKNLRVCGDCHNAMKYISTVSGREIVLRDLNRFHHFKDGKCSCNDY 849 HSP 2 Score: 251.136 bits (640), Expect = 6.102e-68 Identity = 168/532 (31.58%), Postives = 279/532 (52.44%), Query Frame = -2
Query: 1978 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLD---RMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVL-LDVFVGSSLVDLYAKC-GQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKC---GLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQI-QEAAELFSLMRCKG-VSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG 3543
+S +L C K VH ++I+ +PD L+NSL++ Y+K G A V + R +RDVVSW+A+ + N G D + ++ + G++PN +C ++ACS S + G + G +K G DV VG SL+D++ K E A +VF M + NVV+W ++ G + + F M S KFTLS+V +C L NL+ G HS I++G D V C L+D+Y+KC G +D KVF R+++ ++++W+A+I + + EA LFS M +G V PN TF+S NL D + V FK G S ++N++I+M++K +++ R F+ +S N S+N+ L G+ ++ + ++ TF S+L +N+ +I+ G Q+H+ ++K GL N V +LI +Y+K G + A +F + R++ SWT++I G+A+ + + ++ FN M +GV PN T LS+CS++ ++ G
Sbjct: 65 FSSLLKSCIRARDFRLGKLVHARLIEFDIEPDSVLYNSLISLYSKSGDSAKAEDVFETMRRFGKRDVVSWSAMMACYGNNGRELDAIKVFVEFLELGLVPNDYCYTAVIRACSNSDFVGVGRVTLGFLMKTGHFESDVCVGCSLIDMFVKGENSFENAYKVFDKMSELNVVTWTLMITRCMQMGFPREAIRFFLDMVLSGFESDKFTLSSVFSACAELENLSLGKQLHSWAIRSGLVDD--VECSLVDMYAKCSADGSVDDCRKVFDRMEDHSVMSWTALITGYMKNCNLATEAINLFSEMITQGHVEPNHFTFSSAFKACGNLSDPRVGKQVLGQAFKRGLASNSSVANSVISMFVKSDRMEDAQRAFESLSEKNLVSYNTFLDGTCRNLNFEQAFKLLSEITERELGVSAFTFASLLSGVANVGSIRKGEQIHSQVVKLGLSCNQPVCNALISMYSKCGSIDTASRVFNFMENRNVISWTSMITGFAKHGFAIRVLETFNQMIEEGVKPNEVTYVAILSACSHVGLVSEG 594 HSP 3 Score: 73.9442 bits (180), Expect = 6.574e-12 Identity = 38/126 (30.16%), Postives = 67/126 (53.17%), Query Frame = -2
Query: 3214 LFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACS 3591
L E T+ E ++ +L A+ GS+ + +H +++K G + + N+L++ Y+K G A +V + M R+V+SWT++ +G G+ VL+ + M ++GV PN V L ACS
Sbjct: 461 LLSEITERELGVSAFTFASLLSGVANVGSIRKGEQIHSQVVKLGLSCNQPVCNALISMYSKCGSIDTASRVFNFMENRNVISWTSMITGFAKHGFAIRVLETFNQMIEEGVKPNEVTYVAILSACS 586
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP320_ARATH (Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis thaliana GN=DOT4 PE=2 SV=1) HSP 1 Score: 460.299 bits (1183), Expect = 5.934e-142 Identity = 269/820 (32.80%), Postives = 442/820 (53.90%), Query Frame = -2
Query: 922 ERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASI-AGVA----ANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHM-QRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3363
+R V G + + L C K + P C V L+ C+ S L G + G ++D +GS L +Y CG ++ A RVF + + + WN L+N A GD + LF+ M S + +T S V KS + L ++ G H ++K+G VG L+ Y K + KVF + E ++++W+++I+ G ++ +F M G+ + T S+ AG A +LG ++ GV AC F E N L+ MY K + + VF+EMS + S+ S+++G +F +M EG PD+ T T++L C+ + G +VH I +N L + V +L+D+YAK G + +AEL+F+++ +D+ SW +I GY+++ + +A+ FN + + + +P+ T+A L +C++++ + G+ +H ++ G D V++S+VDMYA CG + A F + S D VSW +M+ GY H G + + F M G+E DE++F+++L ACS+ G V+EG +F+ + + I PT++H AC++D+L R G + FI++M + + IW +L CR+H + KL E AEK+FEL P +Y+L+ANI+A +WE V+R+R + G++K PGCSW+ + G+V+IF + D +P+ I L+++ + I GY P T + L D + EK E L H+E+LA+A +IS K IR+ KNL +C DCH+ K +S + ++EIV+RD+N FH FK G CSC+ +
Sbjct: 58 DRSVTDANTQLRRFCESGNLENAVKLLCVSGKWDIDPRTLCSV--LQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKAC-----FSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPDATIWGALLCGCRIHHDVKLAEKVAEKVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIEIKGRVNIFVAGDSSNPETENIEAFLRKVRARMIEEGYSPLTKYALIDAEEMEKEEALCGHSEKLAMALGIISSGHGKIIRVTKNLRVCGDCHEMAKFMSKLTRREIVLRDSNRFHQFKDGHCSCRGF 870 HSP 2 Score: 234.187 bits (596), Expect = 2.832e-62 Identity = 182/614 (29.64%), Postives = 298/614 (48.53%), Query Frame = -2
Query: 1708 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEG--VHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEG-FKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLS 3534
+L CA SL K V I GF D +L + L Y G A +V D + + W L + L G + L+ M GV + + K+ S + G LHG +K G VG+SLV Y K +++ A +VF M +++V+SWN+++NGY G A K L++F M S + T+ +V C ++ G HS+ +K ++ LLD+YSKCG + VF+ + + ++V++++MI +G EA +LF M +G+SP+ +T ++ A RL EG VH I ++ +I +SNAL+ MY K S+Q VF EM + SWN+++ G + S+F+ +L E F PD T +L +C++L G ++H +I++NG + V SL+D+YAK G L A +LF + +DL SWT +IAGY ++AI FN M++ G+ + + + L +CS+ +++ G + + M + V + IVDM A G + +A M + D W +L G H +K+ E + + LEP+ + +++
Sbjct: 100 VLQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARY--RLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPDATIWGALLCGCRIHHD-VKLAEKVAEKVFE-LEPENTGYYVLMA 709 HSP 3 Score: 112.464 bits (280), Expect = 1.067e-23 Identity = 82/302 (27.15%), Postives = 148/302 (49.01%), Query Frame = -2
Query: 2725 VPLFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLY-CSMKKDGVLPNGHCLVTALKACSLSLDLF-FGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTY--LVNLTW---GLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQE----PNIVAWSAMI 3597
V LF+E + + + + +L+ CA L K VH I + D+ + N+L++ YAK G A V M +D++SW + G Y + L L+ +++ P+ + L AC+ SL F G +HG ++ G D V +SLVD+YAKCG + LA +F + K++VSW ++ GY + G + + LF M ++ + + + ++L +C++ LV+ W ++ H I+ EH C++D+ ++ G D +K ++ I+ P+ W A++
Sbjct: 382 VKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACA-SLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEH----YACIVDMLARTG---DLIKAYRFIENMPIPPDATIWGALL 675
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP206_ARATH (Putative pentatricopeptide repeat-containing protein At2g01510 OS=Arabidopsis thaliana GN=PCMP-H36 PE=3 SV=1) HSP 1 Score: 458.373 bits (1178), Expect = 9.262e-142 Identity = 258/743 (34.72%), Postives = 391/743 (52.62%), Query Frame = -2
Query: 922 SSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCS---KFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLK--VFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNP-GIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3132
++++ + K G + A +F M + VV+W L+ YA + LF+ M S C+ T + +L C V H+ +K G + + F+ + L S C + L +F+ I E + V ++ +I ++ G E+ LF MR G P+ TF+ + L D + +HA GF + + N ++ Y K V +F EM + S+N ++S + F +M GF F ++L +NL +++ G Q+H + VG SL+D+YAK +AEL+F L +R SWTALI+GY Q +K F M+ + + T AT L + ++ A L G+ LH+ I++GN +VF S +VDMYA CG I++A F M + VSWN ++ + + G + F M++ GL+PD V+ + VL+ACS+ GFV +G EYF +++ ++GI P KH ACM+D+LGR G+F E E + +M + ++W +VL CR+H N L E AAEKLF + A+ Y+ ++NI+AA G WE VR V+ M G+KK P SWV VN ++H+F S D HP EI K+ EL + GY P T ++ DV + K+E L +H+ERLA+AFALIS I + KNL C DCH +KL+S ++K+EI VRD + FHHF G+CSC DY
Sbjct: 83 NTMISGHVKTGDVSSARDLFDAMPDRTVVTWTILMGWYARNSHFDEAFKLFRQMCRSS-SCTLPDHVTFTTLLPGCNDAVPQNAVGQVHAFAVKLGFDTNPFLTVSNVLLKSYCEVRRLDLACVLFEEIPEKDSVTFNTLITGYEKDGLYTESIHLFLKMRQSGHQPSDFTFSGVLKAVVGLHDFALGQQLHALSVTTGFSRDASVGNQILDFYSKHDRVLETRMLFDEMPELDFVSYNVVISSYSQADQYEASLHFFREMQCMGFDRRNFPFATMLSIAANLSSLQMGRQLHCQALLATADSILHVGNSLVDMYAKCEMFEEAELIFKSLPQRTTVSWTALISGYVQKGLHGAGLKLFTKMRGSNLRADQSTFATVLKASASFASLLLGKQLHAFIIRSGNLENVFSGSGLVDMYAKCGSIKDAVQVFEEMPDRNAVSWNALISAHADNGDGEAAIGAFAKMIESGLQPDSVSILGVLTACSHCGFVEQGTEYFQAMSPIYGITPKKKHYACMLDLLGRNGRFAEAEKLMDEMPFEPDEIMWSSVLNACRIHKNQSLAERAAEKLFSMEKLRDAAAYVSMSNIYAAAGEWEKVRDVKKAMRERGIKKVPAYSWVEVNHKIHVFSSNDQTHPNGDEIVRKINELTAEIEREGYKPDTSSVVQDVDEQMKIESLKYHSERLAVAFALISTPEGCPIVVMKNLRACRDCHAAIKLISKIVKREITVRDTSRFHHFSEGVCSCGDY 824 HSP 2 Score: 217.238 bits (552), Expect = 5.594e-57 Identity = 184/700 (26.29%), Postives = 316/700 (45.14%), Query Frame = -2
Query: 1543 SKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLP----------NGHCLVTALK---------ACSLSLDLFFGTLL---------------HGEGVKVGVLLDVF--VGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANL-----GDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMK 3516
S + + V +IIK GFD D N +V +RG A KV D MP ++ VS + SG V G DL+ +M V+ N H A K +C+L + F TLL H VK+G + F V + L+ Y + +++LA +F + +K+ V++N L+ GY G + ++LF M +S + S FT S VLK+ L + G H+L + TG D VG +LD YSK +T +F + E + V+++ +I + Q + + F M+C G FA++ +AANL G +L+C+ + A +S + + N+L+ MY K + +FK + + SW +L+SG G +F KM + D TF ++L++ ++ ++ G Q+HA II++G +N G+ L+D+YAK G + DA +F ++ +R+ SW ALI+ +A + E AI F M G+ P++ ++ L++CS+ +E G + +M+ G + + ++D+ G EAE ++M + EPDE+ + +VL+AC + + + L ++ + + + M +I AG++ +V K M+
Sbjct: 25 SPATFLDTRRVDARIIKTGFDTDTCRSNFIVEDLLRRGQVSAARKVYDEMPHKNTVSTNTMISGHVKTGDVSSARDLFDAMPDRTVVTWTILMGWYARNSH-FDEAFKLFRQMCRSSSCTLPDHVTFTTLLPGCNDAVPQNAVGQVHAFAVKLGFDTNPFLTVSNVLLKSYCEVRRLDLACVLFEEIPEKDSVTFNTLITGYEKDGLYTESIHLFLKMRQSGHQPSDFTFSGVLKAVVGLHDFALGQQLHALSVTTGFSRDASVGNQILDFYSKHDRVLETRMLFDEMPELDFVSYNVVISSYSQADQYEASLHFFREMQCMGFDRRNFPFATMLSIAANLSSLQMGRQLHCQALLATA-----DSILHVGNSLVDMYAKCEMFEEAELIFKSLPQRTTVSWTALISGYVQKGLHGAGLKLFTKMRGSNLRADQSTFATVLKASASFASLLLGKQLHAFIIRSGNLENVFSGSGLVDMYAKCGSIKDAVQVFEEMPDRNAVSWNALISAHADNGDGEAAIGAFAKMIESGLQPDSVSILGVLTACSHCGFVEQGTEYFQAMSPIYGITPKKKHYACMLDLLGRNGRFAEAE----------------------------------KLMDEMPFEPDEIMWSSVLNACRIHKNQSLAERAAEKLFSMEKLRDAAAYVS-MSNIYAAAGEWEKVRDVKKAMR 683 HSP 3 Score: 166.777 bits (421), Expect = 8.591e-41 Identity = 123/479 (25.68%), Postives = 225/479 (46.97%), Query Frame = -2
Query: 2119 YSKILHDCASKGSLSSAKAVHGKIIKKGFD--PDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQ-VHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQL-IERDLFSWTALI 3543
++ +L C ++ VH +K GFD P L + N L+ +Y + AC + + +PE+D V++ L +G +G + + L+ M++ G P+ LKA D G LH V G D VG+ ++D Y+K ++ +F M + + VS+N +++ Y+ L+ F+ M F + +L L +L G H + + VG L+D+Y+KC + E+ +FK + + V+W+A+I ++G +LF+ MR + +Q TFA++ +A+ L + +HA I + G + + L+ MY K S+++ +VF+EM N+ SWN+L+S + + F KM+ G +PD + +L +CS+ ++ G + A G+ ++DL +NG A+AE L ++ E D W++++
Sbjct: 150 FTTLLPGCNDAVPQNAVGQVHAFAVKLGFDTNPFLTVSNVLLKSYCEVRRLDLACVLFEEIPEKDSVTFNTLITGYEKDGLYTESIHLFLKMRQSGHQPSDFTFSGVLKAVVGLHDFALGQQLHALSVTTGFSRDASVGNQILDFYSKHDRVLETRMLFDEMPELDFVSYNVVISSYSQADQYEASLHFFREMQCMGFDRRNFPFATMLSIAANLSSLQMGRQLHCQALLATADSILHVGNSLVDMYAKCEMFEEAELIFKSLPQRTTVSWTALISGYVQKGLHGAGLKLFTKMRGSNLRADQSTFATVLKASASFASLLLGKQLHAFIIRSGNLENVFSGSGLVDMYAKCGSIKDAVQVFEEMPDRNAVSWNALISAHADNGDGEAAIGAFAKMIESGLQPDSVSILGVLTACSHCGFVEQGTEYFQAMSPIYGITPKKKHYACMLDLLGRNGRFAEAEKLMDEMPFEPDEIMWSSVL 628 HSP 4 Score: 142.895 bits (359), Expect = 3.678e-33 Identity = 102/340 (30.00%), Postives = 168/340 (49.41%), Query Frame = -2
Query: 2596 LFKEKTKLEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWG---LIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCL---DEQGQIQEAAE-LFSLMRCKGVSPNQHTFASIAGVAANLGD 3591
F+E + +R ++ +L A+ SL + +H + + D LH+ NSLV+ YAK +F A + +P+R VSWTAL SG V +G L L+ M+ + + T LKA + L G LH ++ G L +VF GS LVD+YAKCG ++ A +VF M +N VSWNAL++ +A GD + F M ES L+ ++ VL +C++ + G A S + + + C+LDL + G + K+ + EP+ + WS++++ Q + AAE LFS+ + + + + S++ + A G+
Sbjct: 338 FFREMQCMGFDRRNFPFATMLSIAANLSSLQMGRQLHCQALLATADSILHVGNSLVDMYAKCEMFEEAELIFKSLPQRTTVSWTALISGYVQKGLHGAGLKLFTKMRGSNLRADQSTFATVLKASASFASLLLGKQLHAFIIRSGNLENVFSGSGLVDMYAKCGSIKDAVQVFEEMPDRNAVSWNALISAHADNGDGEAAIGAFAKMIESGLQPDSVSILGVLTACSHCGFVEQGTEYFQAMSPIYGITPKKKHY--ACMLDLLGRNGRFAEAEKLMDEMPFEPDEIMWSSVLNACRIHKNQSLAERAAEKLFSMEKLRDAA----AYVSMSNIYAAAGE 671
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Match: PP285_ARATH (Pentatricopeptide repeat-containing protein At3g57430, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H81 PE=2 SV=2) HSP 1 Score: 457.218 bits (1175), Expect = 1.452e-140 Identity = 269/835 (32.22%), Postives = 441/835 (52.81%), Query Frame = -2
Query: 922 RMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLD-VFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLV---NLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHG-FESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKML-AEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQ-----------RQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPL-IWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3372
R PE W L V + + Y M G+ P+ + LKA + D+ G +H K G +D V V ++LV+LY KCG +VF + ++N VSWN+L++ L F+ M + + S FTL +V+ +C+ L L G H+ ++ G E + F+ L+ +Y K G + + ++V W+ ++ L + Q+ EA E M +GV P++ T +S+ ++L + +HA K+G + + +AL+ MY + V +G RVF M WN++++G + +F M + G + T ++ +C +H ++K GL + V +L+D+Y++ G + A +F ++ +RDL +W +I GY S+ E A+ + MQ R + PN+ TL T L SC+ ++ L G+ +H+ AIK + DV V S++VDMYA CGC++ + F + + ++WN+++ Y H G + ++ RMM+ +G++P+EVTFI+V +ACS+ G V+EG F + +G+ P+ H AC++D+LGRAG+ E + M N W ++LG R+H N ++GE AA+ L +L P +ASHY+LLANI+++ G W+ VR M GV+KEPGCSW+ +VH F + D HP+ ++ L+ L E+ GYVP T +L++V +DEK L H+E+LA+AF +++ +P IR+ KNL +C DCH K +S ++ +EI++RD FH FK+G CSC DY
Sbjct: 60 RSPEW----WIDLLRSKVRSNLLREAVLTYVDMIVLGIKPDNYAFPALLKAVADLQDMELGKQIHAHVYKFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKG-ELNSFIINTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMPRDFNKAGAWSSLLGASRIHNNLEIGEIAAQNLIQLEPNVASHYVLLANIYSSAGLWDKATEVRRNMKEQGVRKEPGCSWIEHGDEVHKFVAGDSSHPQSEKLSGYLETLWERMRKEGYVPDTSCVLHNVEEDEKEILLCGHSEKLAIAFGILNTSPGTIIRVAKNLRVCNDCHLATKFISKIVDREIILRDVRRFHRFKNGTCSCGDY 889 HSP 2 Score: 221.476 bits (563), Expect = 4.243e-58 Identity = 158/575 (27.48%), Postives = 280/575 (48.70%), Query Frame = -2
Query: 1873 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPD-LHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACS---LSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGC-EHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELF-SLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKM------LAEG-----FKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENG-QLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEA 3543
+ +L A + K +H + K G+ D + + N+LVN Y K G FG KV DR+ ER+ VSW +L S L + L+ + M + V P+ LV+ + ACS + L G +H G++ G L+ F+ ++LV +Y K G++ ++ + +++V+WN +L+ + L + M + +FT+S+VL +C++L L G H+ +K G + + FVG L+D+Y C +VF + + I W+AMI + +EA LF + G+ N T A + G E +H + K G + + + N L+ MY ++ + R+F +M + +WN++++G + + + HKM +++G KP+ T +IL SC+ L + G ++HA+ IKN L + VG++L+D+YAK G L + +F Q+ ++++ +W +I Y ++AI M QGV PN T + ++CS+ M++ G ++ + M G + +VD+ G I+EA
Sbjct: 100 FPALLKAVADLQDMELGKQIHAHVYKFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKGE-LNSFIINTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEA 673 HSP 3 Score: 131.724 bits (330), Expect = 1.005e-29 Identity = 76/305 (24.92%), Postives = 152/305 (49.84%), Query Frame = -2
Query: 2668 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMK-----------KDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-----EPNIVAWSAMIDCLDEQGQIQEAAELFSLM 3534
++ C G+ S +A+HG ++K+G D D + N+L++ Y++ G A ++ +M +RD+V+W + +G V + D L L M+ + + PN L+T L +C+ L G +H +K + DV VGS+LVD+YAKCG ++++ +VF + +KNV++WN ++ Y + G+ + ++L + M ++ ++ T +V +C++ G+ ++ L++F ++ EP+ ++ ++D L G+I+EA +L ++M
Sbjct: 411 VVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSH-----------------------------------SGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMM 680 HSP 4 Score: 72.7886 bits (177), Expect = 1.565e-11 Identity = 54/201 (26.87%), Postives = 96/201 (47.76%), Query Frame = -2
Query: 3028 EKTKLEGAKRLKLYSK------ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVG--------SSLVDLYAKCGQMELAERVFLFM--DKKNVVSWNALL 3582
E+ +GA R+ L IL CA+ +L+ K +H IK D+ + ++LV+ YAK G + KV D++P+++V++W + G G + +DL M GV PN ++ ACS S G + EG+++ ++ G + +VDL + G+++ A ++ M D +W++LL
Sbjct: 501 ERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHS-----GMV--DEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMPRDFNKAGAWSSLL 694
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A022Q8N3_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a001176mg PE=4 SV=1) HSP 1 Score: 946.806 bits (2446), Expect = 0.000e+0 Identity = 458/871 (52.58%), Postives = 630/871 (72.33%), Query Frame = -2
Query: 922 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3534
+L DCA+ L+ K +HG II+ G +PDLHLW SL+N YAK G ++ V + M +DVVSWTAL SG V +G+G + ++L+C M+++ V PN L T LK CS+SLDL FG +H E VK+GVL D ++GSSL+DLY+K G+ME A+ VF M +KN + WNALLNGYA G+ +L LF M + E+R S +TLS VLK T G HS+ IK G E D+FV C L+++YSKCG+A LKVFK ++ P+IV WS++I LD++G +EAA+LF M G+ PN+ T +++ A +LGD Y + VHAC K G E + ++SNALIAMYMK+ S+ +G+R+F +M++W+ SWN+LLS + +T DRG IF +ML +GF P++ TF SILRSCS+L NI+FG QVH+HIIKN + VGT+LID+YAK G + D E +F++L E+D+F+WT +I+GY+Q++ EKA + FN M+R+GV PN FTLA+ L CS +A LENG+ LHS+AIKAG S D+FVSS+++DMYA CG I++AEA F GM DTV WN ++ GY QH +G K L+ FR M+++G PD VTF+ +LSACS+MG V EG ++F S++ +GI P+I+H AC++D+LGRAGKF+E+E I++M++T N LIWE VLG C++HGN +LGE AAEKLF+++P S+YILL+NI+A+KGRW DV +VR M+ G+KKEPGCSWV ++ Q+H+F S D HP+ +I+ K++EL E GY+P T ++ ++V++ EK E LF+H+ERLAL FALIS IRIFKNL IC DCH+F+K VS + QEIVVRDA+ FHHF++GICSC+DY
Sbjct: 1 MLRDCAAGMRLNEGKMIHGCIIRSGSEPDLHLWVSLINFYAKCGNLDFSRNVFELMSMKDVVSWTALISGFVAQGHGIESIELFCQMRREDVRPNEFTLATVLKGCSVSLDLNFGKQIHAEAVKIGVLSDAYIGSSLIDLYSKSGEMEYADGVFCMMPEKNNIVWNALLNGYAQVGNGKAVLRLFNEMEDPEMRLSNYTLSIVLKGIASFCAFTAGQGVHSIAIKVGGETDDFVRCSLVNMYSKCGVANYALKVFKMMENPDIVTWSSIICALDQEGLKEEAAKLFHRMMRSGMRPNEFTLSTLVSAATDLGDLRYGQSVHACAHKFGLEFDHLVSNALIAMYMKLGSIYDGYRIFNKMTNWDVISWNALLSAFHDDETSDRGTRIFQQMLTDGFTPNMYTFISILRSCSSLANIEFGKQVHSHIIKNKFVRDGYVGTALIDMYAKCGCMEDVEAIFSRLDEKDVFAWTVIISGYSQTNQGEKAARLFNLMRREGVIPNEFTLASSLRGCSAIASLENGRQLHSLAIKAGQSTDMFVSSALIDMYAKCGYIDDAEALFNGMQPNDTVLWNTIICGYSQHGEGDKALKAFRQMINEGFLPDGVTFLGILSACSHMGLVEEGNKHFYSMSESYGITPSIEHYACLVDVLGRAGKFDEIERLIENMELTPNALIWENVLGACKVHGNVELGERAAEKLFQIDPETDSNYILLSNIYASKGRWNDVSKVRKSMSSKGIKKEPGCSWVEIDAQIHVFLSQDSSHPRLLDIHRKMEELRETVSEVGYIPNTKYVPHNVTEKEKRENLFNHSERLALGFALISKVNGGRIRIFKNLRICGDCHEFIKCVSSTVDQEIVVRDASRFHHFRNGICSCKDY 871 HSP 2 Score: 220.32 bits (560), Expect = 7.586e-56 Identity = 142/441 (32.20%), Postives = 229/441 (51.93%), Query Frame = -2
Query: 2275 LFKEKTKLEGAKRLKLY--SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQ 3591
LF E E RL Y S +L AS + ++ + VH IK G + D + SLVN Y+K GV YA KV M D+V+W+++ L EG + L+ M + G+ PN L T + A + DL +G +H K G+ D V ++L+ +Y K G + R+F M +V+SWNALL+ + + + +FQ M + +T ++L+SC+ L N+ +G HS +IK D +VG L+D+Y+KCG ED +F R+ E ++ AW+ +I + Q ++AA LF+LMR +GV PN+ T AS + + +H+ K G +++ +S+ALI MY K + + +F M ++ WN+++ G D+ F +M+ EGF PD TF IL +CS++ ++ G++
Sbjct: 184 LFNEMEDPE--MRLSNYTLSIVLKGIASFCAFTAGQGVHSIAIKVGGETDDFVRCSLVNMYSKCGVANYALKVFKMMENPDIVTWSSIICALDQEGLKEEAAKLFHRMMRSGMRPNEFTLSTLVSAATDLGDLRYGQSVHACAHKFGLEFDHLVSNALIAMYMKLGSIYDGYRIFNKMTNWDVISWNALLSAFHDDETSDRGTRIFQQMLTDGFTPNMYTFISILRSCSSLANIEFGKQVHSHIIKNKFVRDGYVGTALIDMYAKCGCMEDVEAIFSRLDEKDVFAWTVIISGYSQTNQGEKAARLFNLMRREGVIPNEFTLASSLRGCSAIASLENGRQLHSLAIKAGQSTDMFVSSALIDMYAKCGYIDDAEALFNGMQPNDTVLWNTIICGYSQHGEGDKALKAFRQMINEGFLPDGVTFLGILSACSHMGLVEEGNK 622 HSP 3 Score: 141.739 bits (356), Expect = 7.577e-31 Identity = 83/289 (28.72%), Postives = 142/289 (49.13%), Query Frame = -2
Query: 2695 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWG-----LIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCLDEQGQIQ 3543
+ IL C+S ++ K VH IIK F D ++ +L++ YAK G + R+ E+DV +WT + SG G L+ M+++GV+PN L ++L+ CS L G LH +K G D+FV S+L+D+YAKCG ++ AE +F M + V WN ++ GY+ G+ K L F+ M T +L +C+++ + G ++ S I EH CL+D+ + G ++ ++ + ++ PN + W ++ G ++
Sbjct: 402 FISILRSCSSLANIEFGKQVHSHIIKNKFVRDGYVGTALIDMYAKCGCMEDVEAIFSRLDEKDVFAWTVIISGYSQTNQGEKAARLFNLMRREGVIPNEFTLASSLRGCSAIASLENGRQLHSLAIKAGQSTDMFVSSALIDMYAKCGYIDDAEALFNGMQPNDTVLWNTIICGYSQHGEGDKALKAFRQMINEGFLPDGVTFLGILSACSHMGLVEEGNKHFYSMSESYGITPSIEH----YACLVDVLGRAGKFDEIERLIENMELTPNALIWENVLGACKVHGNVE 686
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A022Q9L0_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a024266mg PE=4 SV=1) HSP 1 Score: 944.88 bits (2441), Expect = 0.000e+0 Identity = 457/871 (52.47%), Postives = 634/871 (72.79%), Query Frame = -2
Query: 922 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3534
+L DCA+ L+ K +HG II+ G +PDLHLW SL+N YAK G ++ V + M +DVVSWTAL SG V +G+G + ++L+C M+++ V PN L T LK CS+SLDL FG +H E VK+GVL DV++GSSL+DLY+K G+ME A+ VF M +KN V WNALLNGYA G+ +L LF M + E+R S +TLS VLK G HS+ IK G E D+FV C L+++YSKCG+A LKVF+ ++ P+IV WS++I LD++G +EAA+LF M G+ PN+ T +++ A +LGD Y + VHAC K G E + +++NALIAMYMK+ S+ +G+R+F +M++W+ SWN+LLSG + +T D+ +F +ML +GF P++ TF SILRSCS+L NI+FG QVH+H+IKN + VGT+LID+YAK G + D E +F++L E+D+F+WT +I+GY+Q++ EKA + FN M+R+GV PN FTLA+ L CS +A LENG+ LHS+AIKAG S D+FVSS+++DMYA CG I++AEA F GM S DTV WN ++ GY QH +G K L+ FR M+++G PD VTF+ +LSACS+MG V EGK++F S++ +GI P+I+H AC++D+LGRAGKF+E+E I++M++T N LIWE VLGTC++HGN +LGE AAEKLF+++P S+YILL+NI+A+KGRW DV RVR M+ G+KKEPGCSW+ ++ Q+H+F S D HP+ +I+ K++EL E+ GY+P T ++ ++V++ EK E LF+H+ERLAL FALIS IRIFKNL IC DCH+F+K VS + QEIVVRDA+ FHHF++GICSC+DY
Sbjct: 1 MLRDCAAGMRLNEGKMIHGCIIRSGSEPDLHLWVSLINFYAKCGALDFSRNVFELMSMKDVVSWTALISGFVAQGHGMESIELFCQMRREDVRPNEFTLSTVLKGCSVSLDLNFGKQIHAEAVKIGVLSDVYIGSSLIDLYSKSGEMEYADGVFRMMPEKNNVLWNALLNGYAQVGNGKAVLRLFNEMEDPEMRFSNYTLSIVLKGIASFGAFIAGQGVHSIAIKVGGETDDFVRCSLVNMYSKCGVANYALKVFETMENPDIVTWSSIICALDQEGLKEEAAKLFHRMMRSGMRPNEFTLSTLVSAATDLGDLRYGQSVHACAHKFGLEFDHLVNNALIAMYMKLGSIYDGYRIFNKMANWDVISWNALLSGFHDDETSDQVTRVFKQMLTDGFTPNMYTFISILRSCSSLSNIEFGKQVHSHVIKNKFVGDGYVGTALIDMYAKCGCMEDVEAIFSRLNEKDVFAWTVIISGYSQTNQGEKAARLFNQMRREGVIPNEFTLASSLRGCSAIASLENGRQLHSLAIKAGQSTDMFVSSALIDMYAKCGHIDDAEALFNGMQSNDTVLWNTIICGYSQHGEGDKALKAFRQMINEGFLPDGVTFLGILSACSHMGLVEEGKKHFYSMSESYGITPSIEHYACLVDVLGRAGKFDEIERLIENMELTPNALIWENVLGTCKVHGNVELGERAAEKLFQIDPETDSNYILLSNIYASKGRWNDVSRVRKSMSSQGIKKEPGCSWLEIDAQIHVFLSQDSSHPRLLDIHRKMEELRERVSEVGYIPNTKYVPHNVTEKEKRENLFNHSERLALGFALISKVNGGRIRIFKNLRICGDCHEFIKCVSSTVDQEIVVRDASRFHHFRNGICSCKDY 871 HSP 2 Score: 274.248 bits (700), Expect = 9.674e-74 Identity = 190/670 (28.36%), Postives = 325/670 (48.51%), Query Frame = -2
Query: 1546 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNAL---LNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQL-LHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVP-TIKHCACMIDILGRAGKFNEVESFIKDM 3540
S +L C+ L+ K +H + +K G D+++ +SL++ Y+K G YA V MPE++ V W AL +G G G VL L+ M+ + + + L LK + G +H +KVG D FV SLV++Y+KCG A +VF M+ ++V+W+++ L+ LK +A K LF M S +R ++FTLS ++ + T L +L +G H+ K G E D V L+ +Y K G D ++F ++ ++++W+A++ + + +F M G +PN +TF SI ++L + + + VH+ + K+ F + + ALI MY K +++ +F ++ + +W ++SG ++ +F++M EG P+ T S LR CS + +++ G Q+H+ IK G + V ++LID+YAK G + DAE LF + D W +I GY+Q +KA+K F M +G P+ T LS+CS+M ++E G+ +SM+ G + + + +VD+ G +E E R++ + L P+ + + VL C G V G+ + L F I P T + + +I G++N+V K M
Sbjct: 100 STVLKGCSVSLDLNFGKQIHAEAVKIGVLSDVYIGSSLIDLYSKSGEMEYADGVFRMMPEKNNVLWNALLNGYAQVGNGKAVLRLFNEMEDPEMRFSNYTLSIVLKGIASFGAFIAGQGVHSIAIKVGGETDDFVRCSLVNMYSKCGVANYALKVFETMENPDIVTWSSIICALDQEGLKEEAAK---LFHRMMRSGMRPNEFTLSTLVSAATDLGDLRYGQSVHACAHKFGLEFDHLVNNALIAMYMKLGSIYDGYRIFNKMANWDVISWNALLSGFHDDETSDQVTRVFKQMLTDGFTPNMYTFISILRSCSSLSNIEFGKQVHSHVIKNKFVGDGYVGTALIDMYAKCGCMEDVEAIFSRLNEKDVFAWTVIISGYSQTNQGEKAARLFNQMRREGVIPNEFTLASSLRGCSAIASLENGRQLHSLAIKAGQSTDMFVSSALIDMYAKCGHIDDAEALFNGMQSNDTVLWNTIICGYSQHGEGDKALKAFRQMINEGFLPDGVTFLGILSACSHMGLVEEGKKHFYSMSESYGITPSIEHYACLVDVLGRAGKFDEIE----------------------------------RLIENMELTPNALIWENVLGTCKVHGNVELGERAAEKL---FQIDPETDSNYILLSNIYASKGRWNDVSRVRKSM 729 HSP 3 Score: 142.51 bits (358), Expect = 4.438e-31 Identity = 82/289 (28.37%), Postives = 142/289 (49.13%), Query Frame = -2
Query: 2695 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWG-----LIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCLDEQGQIQ 3543
+ IL C+S ++ K VH +IK F D ++ +L++ YAK G + R+ E+DV +WT + SG G L+ M+++GV+PN L ++L+ CS L G LH +K G D+FV S+L+D+YAKCG ++ AE +F M + V WN ++ GY+ G+ K L F+ M T +L +C+++ + G ++ S I EH CL+D+ + G ++ ++ + ++ PN + W ++ G ++
Sbjct: 402 FISILRSCSSLSNIEFGKQVHSHVIKNKFVGDGYVGTALIDMYAKCGCMEDVEAIFSRLNEKDVFAWTVIISGYSQTNQGEKAARLFNQMRREGVIPNEFTLASSLRGCSAIASLENGRQLHSLAIKAGQSTDMFVSSALIDMYAKCGHIDDAEALFNGMQSNDTVLWNTIICGYSQHGEGDKALKAFRQMINEGFLPDGVTFLGILSACSHMGLVEEGKKHFYSMSESYGITPSIEH----YACLVDVLGRAGKFDEIERLIENMELTPNALIWENVLGTCKVHGNVE 686
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A022R7Q5_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a001179mg PE=4 SV=1) HSP 1 Score: 944.11 bits (2439), Expect = 0.000e+0 Identity = 457/871 (52.47%), Postives = 633/871 (72.68%), Query Frame = -2
Query: 922 ILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3534
+L DCA+ L+ K +HG II+ G +PDLHLW SL+N YAK G ++ V + M +DVVSWTAL SG V +G+G + ++L+C M+++ V PN L T LK CS+SLDL FG +H E VK+ VL DV++GSSL+DLY+K G+ME A+ VF M +KN V WNALLNGYA G+ +L LF M + E+R S +TLS VLK G HS+ IK G E D+FV C L+++YSKCG+A LKVF+ ++ P+IV WS++I LD++G +EAA+LF M G+ PN+ T +++ A +LGD Y + VHAC K G E + +++NALIAMYMK+ S+ +G+R+F +M++W+ SWN+LLSG + +T D+ +F +ML +GF P++ TF SILRSCS+L NI+FG QVH+H+IKN + VGT+LID+YAK G + D E +F++L E+D+F+WT +I+GY+Q++ EKA + FN M+R+GV PN FTLA+ L CS +A LENG+ LHS+AIKAG S D+FVSS+++DMYA CG I++AEA F GM S DTV WN ++ GY QH +G K L+ FR M+++G PD VTF+ +LSACS+MG V EGK++F S++ +GI P+I+H AC++D+LGRAGKF+E+E I+DM++T N LIWE VLGTC++HGN +LGE AAEKLF+++P S+YILL+NI+A+KGRW DV RVR M+ G+KKEPGCSW+ ++ Q+H+F S D HP+ +I+ K++EL E+ GY+P T ++ ++V++ EK E LF+H+ERLAL FALIS IRIFKNL IC DCH+F+K VS + QEIVVRDA+ FHHF++GICSC+DY
Sbjct: 1 MLRDCAAGMRLNEGKMIHGCIIRSGSEPDLHLWVSLINFYAKCGALDFSRNVFELMSMKDVVSWTALISGFVAQGHGMESIELFCQMRREDVRPNEFTLSTVLKGCSVSLDLNFGKQIHAEAVKIAVLSDVYIGSSLIDLYSKSGEMEYADGVFRMMPEKNNVLWNALLNGYAQVGNGKAVLRLFNEMEDPEMRFSNYTLSIVLKGIASFGAFIAGQGVHSIAIKVGGETDDFVRCSLVNMYSKCGVANYALKVFETMENPDIVTWSSIICALDQEGLKEEAAKLFHRMMRSGMRPNEFTLSTLVSAATDLGDLRYGQSVHACAHKFGLEFDHLVNNALIAMYMKLGSIYDGYRIFNKMANWDVISWNALLSGFHDDETSDQVTRVFKQMLTDGFTPNMYTFISILRSCSSLSNIEFGKQVHSHVIKNKFVGDGYVGTALIDMYAKCGCMEDVEAIFSRLNEKDVFAWTVIISGYSQTNQGEKAARLFNQMRREGVIPNEFTLASSLRGCSAIASLENGRQLHSLAIKAGQSTDMFVSSALIDMYAKCGHIDDAEALFNGMQSNDTVLWNTIICGYSQHGEGDKALKAFRQMINEGFLPDGVTFLGILSACSHMGLVEEGKKHFYSMSESYGITPSIEHYACLVDVLGRAGKFDEIERLIEDMELTPNALIWENVLGTCKVHGNVELGERAAEKLFQIDPETDSNYILLSNIYASKGRWNDVSRVRKSMSSQGIKKEPGCSWLEIDAQIHVFLSQDSSHPRLLDIHRKMEELRERVSEVGYIPNTKYVPHNVTEKEKRENLFNHSERLALGFALISKVNGGRIRIFKNLRICGDCHEFIKCVSSTVDQEIVVRDASRFHHFRNGICSCKDY 871 HSP 2 Score: 273.478 bits (698), Expect = 1.451e-73 Identity = 190/670 (28.36%), Postives = 324/670 (48.36%), Query Frame = -2
Query: 1546 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNAL---LNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQL-LHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVP-TIKHCACMIDILGRAGKFNEVESFIKDM 3540
S +L C+ L+ K +H + +K D+++ +SL++ Y+K G YA V MPE++ V W AL +G G G VL L+ M+ + + + L LK + G +H +KVG D FV SLV++Y+KCG A +VF M+ ++V+W+++ L+ LK +A K LF M S +R ++FTLS ++ + T L +L +G H+ K G E D V L+ +Y K G D ++F ++ ++++W+A++ + + +F M G +PN +TF SI ++L + + + VH+ + K+ F + + ALI MY K +++ +F ++ + +W ++SG ++ +F++M EG P+ T S LR CS + +++ G Q+H+ IK G + V ++LID+YAK G + DAE LF + D W +I GY+Q +KA+K F M +G P+ T LS+CS+M ++E G+ +SM+ G + + + +VD+ G +E E R++ D L P+ + + VL C G V G+ + L F I P T + + +I G++N+V K M
Sbjct: 100 STVLKGCSVSLDLNFGKQIHAEAVKIAVLSDVYIGSSLIDLYSKSGEMEYADGVFRMMPEKNNVLWNALLNGYAQVGNGKAVLRLFNEMEDPEMRFSNYTLSIVLKGIASFGAFIAGQGVHSIAIKVGGETDDFVRCSLVNMYSKCGVANYALKVFETMENPDIVTWSSIICALDQEGLKEEAAK---LFHRMMRSGMRPNEFTLSTLVSAATDLGDLRYGQSVHACAHKFGLEFDHLVNNALIAMYMKLGSIYDGYRIFNKMANWDVISWNALLSGFHDDETSDQVTRVFKQMLTDGFTPNMYTFISILRSCSSLSNIEFGKQVHSHVIKNKFVGDGYVGTALIDMYAKCGCMEDVEAIFSRLNEKDVFAWTVIISGYSQTNQGEKAARLFNQMRREGVIPNEFTLASSLRGCSAIASLENGRQLHSLAIKAGQSTDMFVSSALIDMYAKCGHIDDAEALFNGMQSNDTVLWNTIICGYSQHGEGDKALKAFRQMINEGFLPDGVTFLGILSACSHMGLVEEGKKHFYSMSESYGITPSIEHYACLVDVLGRAGKFDEIE----------------------------------RLIEDMELTPNALIWENVLGTCKVHGNVELGERAAEKL---FQIDPETDSNYILLSNIYASKGRWNDVSRVRKSM 729 HSP 3 Score: 141.739 bits (356), Expect = 6.543e-31 Identity = 82/289 (28.37%), Postives = 142/289 (49.13%), Query Frame = -2
Query: 2695 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWG-----LIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQ-EPNIVAWSAMIDCLDEQGQIQ 3543
+ IL C+S ++ K VH +IK F D ++ +L++ YAK G + R+ E+DV +WT + SG G L+ M+++GV+PN L ++L+ CS L G LH +K G D+FV S+L+D+YAKCG ++ AE +F M + V WN ++ GY+ G+ K L F+ M T +L +C+++ + G ++ S I EH CL+D+ + G ++ ++ + ++ PN + W ++ G ++
Sbjct: 402 FISILRSCSSLSNIEFGKQVHSHVIKNKFVGDGYVGTALIDMYAKCGCMEDVEAIFSRLNEKDVFAWTVIISGYSQTNQGEKAARLFNQMRREGVIPNEFTLASSLRGCSAIASLENGRQLHSLAIKAGQSTDMFVSSALIDMYAKCGHIDDAEALFNGMQSNDTVLWNTIICGYSQHGEGDKALKAFRQMINEGFLPDGVTFLGILSACSHMGLVEEGKKHFYSMSESYGITPSIEH----YACLVDVLGRAGKFDEIERLIEDMELTPNALIWENVLGTCKVHGNVE 686
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A061GMX3_THECC (Tetratricopeptide repeat (TPR)-like superfamily protein OS=Theobroma cacao GN=TCM_037698 PE=4 SV=1) HSP 1 Score: 883.248 bits (2281), Expect = 0.000e+0 Identity = 437/777 (56.24%), Postives = 573/777 (73.75%), Query Frame = -2
Query: 1213 YSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVE 3543
YS +L D AS GSL+ KA+HG++IK G L W SL++ Y K G FG AC+VLD MP R+V+SWT L S LV+EG+ Y ++LYC+MKKDGV PNG LVTALKACS+ +L FGT LH E +K+GVLLD FV S++VDLYA+CG+M+ AER F M +KNVV+W+A LNGYA GD+ ++L LF GM +E + SKF LS V KSC L NL G + H L IK G + DE++GCC++D+YSKCG+ ED +VF+ I+ PN+VAW++MI+CLD QG+ QEAAELF LMR K V PNQ TFASI A+N D YC+ +HACI K GFES+ LSNALI MYMK++SVQNGW VF MSSW+S SWNSLLSG ++ +T +GP IF KMLAEGF+PD+ TF SILRS L N FG QVHAHI+KNGL NN VGTSLID+YAKN L +AELLF +L ERDLFSWT +IA YAQ+D EKA+K FN MQ++G PN FTL+ C S C N+ MLE+G LHSM+IK+G D +V+S+++DM+ CIE+AEA F M +TVSW+ ++ Y QH QG +E F++MLD+G PDEVTFI VLSACS +G + E K YFDS++ V+ I PT++HCACM+++ RAGKF EVE F+K+M +T N +IW+TVL C++H N + GE AA KLFEL P + +Y+ L+N+FAA+G W++V ++RALM + G+KK+PG SW+ ++G+ ++F + D +P EI +++++L++
Sbjct: 35 YSGLLRDSASNGSLNFGKAIHGQLIKIG----LTSWTSLLHFYTKCGSFGCACQVLDEMPNRNVMSWTVLISRLVSEGHSYRAMNLYCNMKKDGVRPNGFTLVTALKACSMGFELDFGTQLHAEVIKIGVLLDTFVASAVVDLYARCGEMKHAERAFFCMPEKNVVTWSAFLNGYAKMGDSQEVLKLFCGMKGTETKFSKFILSTVFKSCARLGNLQGGRVVHCLAIKIGSDIDEYLGCCIIDMYSKCGVGEDAQRVFEAIKVPNVVAWTSMINCLDGQGKSQEAAELFCLMRQKEVRPNQLTFASIVRTASNFSDLHYCQSIHACILKFGFESDKFLSNALITMYMKMKSVQNGWEVFNAMSSWDSTSWNSLLSGPRDCETYHQGPRIFCKMLAEGFRPDVYTFASILRSNGTLENFGFGKQVHAHIMKNGLDCNNFVGTSLIDMYAKNRCLDEAELLFNELNERDLFSWTVIIASYAQADQGEKAVKWFNRMQQEGFKPNEFTLSCCFSGCHNLRMLESGLQLHSMSIKSGLLNDTYVASALIDMHGKSRCIEDAEAIFKEMDFRNTVSWSTIICAYTQHGQGKMAIEAFKVMLDEGFLPDEVTFIGVLSACSQLGLIEEAKMYFDSISKVYMISPTVEHCACMVNVFCRAGKFEEVERFMKEMNLTENAMIWDTVLWGCKLHSNVEFGERAARKLFELEPELEYNYVTLSNMFAARGEWDEVEKIRALMCNQGIKKQPGGSWLEIHGEAYMFYAQDTSYPGIKEINMQMEKLIK 807 HSP 2 Score: 167.933 bits (424), Expect = 3.122e-39 Identity = 116/418 (27.75%), Postives = 209/418 (50.00%), Query Frame = -2
Query: 2299 LYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEG--VHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNL 3546
+ S + CA G+L + VH IK G D D +L +++ Y+K GV A +V + + +VV+WT++ + L +G + +L+C M++ V PN + ++ S DL + +H +K G D F+ ++L+ +Y K ++ VF M + SWN+LL+G + +F M R +T +++L+S L N +G H+ ++K G + + FVG L+D+Y+K ++ +F + E ++ +W+ +I + Q ++A + F+ M+ +G PN+ T + NL R+ G +H+ K G ++ +++ALI M+ K R +++ +FKEM N+ SW++++ F ML EGF PD TF +L +CS L
Sbjct: 232 ILSTVFKSCARLGNLQGGRVVHCLAIKIGSDIDEYLGCCIIDMYSKCGVGEDAQRVFEAIKVPNVVAWTSMINCLDGQGKSQEAAELFCLMRQKEVRPNQLTFASIVRTASNFSDLHYCQSIHACILKFGFESDKFLSNALITMYMKMKSVQNGWEVFNAMSSWDSTSWNSLLSGPRDCETYHQGPRIFCKMLAEGFRPDVYTFASILRSNGTLENFGFGKQVHAHIMKNGLDCNNFVGTSLIDMYAKNRCLDEAELLFNELNERDLFSWTVIIASYAQADQGEKAVKWFNRMQQEGFKPNEFTLSCCFSGCHNL--RMLESGLQLHSMSIKSGLLNDTYVASALIDMHGKSRCIEDAEAIFKEMDFRNTVSWSTIICAYTQHGQGKMAIEAFKVMLDEGFLPDEVTFIGVLSACSQL 647
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: M5WFX0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021952mg PE=4 SV=1) HSP 1 Score: 825.469 bits (2131), Expect = 0.000e+0 Identity = 416/776 (53.61%), Postives = 547/776 (70.49%), Query Frame = -2
Query: 1285 VPLFKEKTKLEGAK-----RLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVH 3597
VPL+ + L A R ++ C SKG L+ KA+ ++IK G P L + +S+ + + K F +A KV+D +PE+D V W L S L +EG YD++ YC M+KDG +PNG L LKACS+SL+L FGT LH E +K+GV LD VGS+LVDLYAKCG++ELA +VF M KKN VSWNALL+GY GD +IL LF + L+ SKFTL +LKSC ++ NL G H+L+IK GCE D+ +G CLL++YSKC LA+D LKVF RI+ P IVAWS MI CLD+QG+ EAAE+F MR + PNQ T AS+ A NLGD Y E +HAC+FK+GFES+ +SNAL+ MYMK+ SVQ GW F +M ++ASWN LL G + + CD GP++F +MLA+GFKPD T+ SILR CS+LL + F QVH HIIK+GL N V T LI +Y+K L DA+++ +L+ERDLF+WT LI+G AQ++ EKA+K FN MQRQGV PNNFT ++CLS+CS+ A+LE+GQ LHS+A+K+G S D +VS ++V MYA C CIE+AE F G+ S + VSWN ++ GY QH QG K LE F++MLD+G+ PDEVTFI VLSACS+MG +++GK +F+SL +G+ +I+HCACM++I RAGKFNEVE F+ + K+T +PLIWETVL C+MHGN + GE AA+KLFEL P + +YILL++I+AA G+W+DV RVRALM + K PGCSW+ N Q H
Sbjct: 52 VPLYPMQKPLLLAMTLNTVRRRVLMSAFRTCTSKGPLTEGKAIQDQLIKNGIHPSLEICSSMDSLFVKLRSFAFAGKVVDELPEQDAVLWNKLMSRLEDEGCSYDLIKFYCQMRKDGSMPNGLSLAAGLKACSISLELDFGTQLHAEVIKLGVFLDGIVGSALVDLYAKCGELELANKVFFNMPKKNAVSWNALLDGYGKIGDWKEILTLFCRLKIQGLKFSKFTLLTMLKSCAHMENLGGGQAVHALLIKIGCELDKILGSCLLNMYSKCELADDALKVFGRIKNPKIVAWSTMISCLDQQGRSLEAAEMFCQMRHTNLRPNQFTLASMVTAAKNLGDWHYGESIHACVFKYGFESDNYVSNALVTMYMKVGSVQKGWHAFNQMPVRDTASWNFLLCGIYDSENCDHGPNVFKEMLAQGFKPDTYTYISILRCCSSLLTVFFAKQVHTHIIKSGLNANRFVATVLIGMYSKGRSLDDADVILNELMERDLFTWTVLISGCAQTNQGEKAVKSFNQMQRQGVKPNNFTFSSCLSACSSSAILESGQQLHSLALKSGQSNDTYVSCALVAMYAQCRCIEDAEKIFKGLDSRNRVSWNTIICGYSQHGQGKKALEAFQIMLDEGVPPDEVTFIGVLSACSHMGLIDQGKMHFNSLRKEYGLTLSIEHCACMVNIFSRAGKFNEVERFVGEWKLTESPLIWETVLWACKMHGNVEFGERAAQKLFELEPAMGFNYILLSHIYAANGQWDDVARVRALMRSRKITKVPGCSWLEFNAQAH 827
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: S8CXA1_9LAMI (Uncharacterized protein OS=Genlisea aurea GN=M569_02714 PE=4 SV=1) HSP 1 Score: 823.157 bits (2125), Expect = 0.000e+0 Identity = 420/878 (47.84%), Postives = 584/878 (66.51%), Query Frame = -2
Query: 922 SKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLF-QGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKT-GCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALIS-INPVKTIRIFKNLHICEDCHDFMKLVSGVIK--QEIVVRDANCFHHFKSGICSCQDY 3540
S L DC+ + S + K++H +I+ +PD HLW SL+N Y+K G A +V D+MP + VVSWTAL SG V++G + ++LYC+MK +GV PN L T LK S+S D G LH + VK GVL D ++ S+L+DLYAK +M A VF + +K V WN +LNGYA +G+ K+L LF + TE E+ TLS V K L +L HS+ IK G E D+FV C L+++YSK G ED K+F I P+ VAW ++I D QG +E+ +F M+ GV PNQ T +S+ A ++GD Y +H+C +K GFES++++SNALI MY + S+ +G+ +F M + + SWNSLLSG N + D+G IF +ML EGF P++ TF SILR+ S+ N++ G Q+H+ ++KN D+ +G +LID+Y K G + DAE +F +L E+D+FSWTA+I+G++QS+ EKA FN M+R+G PN FTLA+CL +CS+++ LE G+ LHS++IK+GNS DVF +SS++DMY CGC+++AE AF G+ + D V WN ++ GY QH G K F+ ML G+ PD+VTFI +LSACS G V E + +F S+ +GI P I+H ACM+D+ GRAGKF++VE+ I++M V + IWE VLG C++HGN +LGE AA KLFEL+P S+YILL+N+++ RW D RVRA MT GVKKEPGCSW+ ++ +VH+F S D HP+ EI KL+EL ++ AGYV + +L +V++ EK E LFHH+ERLAL F+LI N IRIFKNL IC DCH+FMK VS + +EIV+RDA FHHF++G CSC+DY
Sbjct: 88 SDKLRDCSLQMSTTRGKSIHCFVIRNEIEPDSHLWISLINFYSKCGYLRCARQVFDKMPAKCVVSWTALISGFVSQGMVAESIELYCAMKAEGVRPNEFTLATVLKGSSMSSDSQTGRQLHADVVKHGVLSDAYIASALIDLYAKFEEMPDAYEVFHMLPRKTPVLWNVMLNGYAEQGNGEKVLELFCEITTEPEISFDNCTLSIVFKGVVGLEDLRVCRAVHSMAIKAKGEEFDDFVRCSLVNMYSKFGKTEDAYKIFLTINRPDSVAWCSIISMFDRQGSKEESVRMFRKMQRSGVRPNQFTLSSVLSAATDIGDVRYGRSIHSCAYKLGFESDVLVSNALIGMYAEFGSIHDGYSLFDRMVNPDVVSWNSLLSGFHNDGS-DQGIKIFRQMLVEGFNPNVHTFISILRASSSSSNVETGKQIHSLVVKNSWADHAHIGAALIDVYTKCGCIEDAETVFKRLDEKDVFSWTAMISGHSQSNRGEKAAHLFNRMRREGFVPNEFTLASCLRACSSISSLELGRQLHSLSIKSGNSDDVFAASSLIDMYGKCGCMDDAEKAFDGIPAGDAVLWNTIICGYSQHGDGEKAFGAFKDMLAAGVLPDKVTFIGILSACSRRGLVEEARSHFSSMAETYGITPEIEHYACMVDVFGRAGKFDDVEATIEEMDVAPDSSIWENVLGACKLHGNIELGEKAARKLFELDPDTDSNYILLSNLYSGGKRWTDASRVRASMTGKGVKKEPGCSWMEIDSRVHVFLSGDVSHPRLAEIQDKLEELNRGAVEAGYVANVEIVLRNVTESEKRESLFHHSERLALGFSLIGKANGGGKIRIFKNLRICADCHEFMKFVSMTMSPAREIVIRDAKRFHHFRNGACSCRDY 964
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: V4WDV0_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10007442mg PE=4 SV=1) HSP 1 Score: 808.52 bits (2087), Expect = 0.000e+0 Identity = 400/756 (52.91%), Postives = 545/756 (72.09%), Query Frame = -2
Query: 1294 KRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFK-RIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNG 3558
KRL+ YS +LHD A+KGSL K +HG +IK G + D HLW SL+NAYAK G YA +VL+ MP+RDVVSW+AL +G V +GYG D + L+C+++++GV PN L T LKACS+S D+ FG +H E VK+G D+FVGS+LVDLYAKC MELAERVF M + NVVSWNALLNGYA GD K+++LF M + E + SKF+LS VLK + G + H++ I+ GC D+F+ C L+D+YSKCGLA++ LKVF RI++P++VAW A+I CLD+QG QE A++F+L R V PNQ S+ GD+ E +HA I K+GFES+ ++ NAL++MYM+ V G +VF+ ++ +S SWN+LLSG Q+ ++ D+G F++ML +GFKP++CTF IL++CS+L ++ FG Q+HAH IK+ L N++VGTSL+D+YAK+G L DA + F L +DLF++TA+I YAQ+ +E A+KCF M+ +G+ PN FTLA+CL+ CS +A L NG+LLHS+A+K G+ D+FVS+++V MYA CG I++AEA F G V DT SWNMM+ GY++H G K LE FRMMLD+G PDE+TF+ VLSACS+MG + EGK++F S+ ++GI PTIKH ACMIDILGRAGKF E+E+FI + K+T N L+WE +LG C HGN +L E AEKL EL P + S+Y+ ++I A +GRW D VRAL++ G+KKEP CSW+ V+G
Sbjct: 94 KRLREYSSLLHDSAAKGSLKEGKLIHGHVIKCGLELDSHLWVSLINAYAKCGTVVYAQRVLNIMPQRDVVSWSALITGFVAQGYGSDGVCLFCAIQREGVRPNEFALATCLKACSMSHDVGFGKQVHLEAVKLGFFSDLFVGSALVDLYAKCSGMELAERVFKSMPELNVVSWNALLNGYAESGDGQKVMHLFCSMKDMEKKFSKFSLSTVLKGFANSGYIKAGQVVHAMAIRLGCALDKFLSCSLVDMYSKCGLADNALKVFYIRIKDPDVVAWGAIITCLDQQGCCQEVAKIFNLTRESSVKPNQFVLTSLVRATTETGDQRCGESIHAVICKYGFESDTLVGNALVSMYMENGCVSYGSQVFEAIAHQDSVSWNALLSGFQDYESPDQGLRTFYQMLLKGFKPNMCTFIVILKACSSLSDVGFGKQLHAHTIKHSLDGNHVVGTSLVDMYAKSGCLEDAGVAFDSLANKDLFAYTAIITSYAQAGEAEMALKCFRKMRLEGIKPNEFTLASCLNGCSPVATLANGRLLHSIAVKTGHLLDMFVSTALVAMYAKCGSIDDAEAVFKGSVLRDTASWNMMIGGYVKHGLGEKALEAFRMMLDEGYVPDEITFVVVLSACSHMGLIEEGKKHFSSIKKIYGITPTIKHFACMIDILGRAGKFTEIENFITETKLTPNALVWENLLGACSWHGNIELDEKDAEKLLELEPKMESNYVFPSDISATQGRWNDFSGVRALLSSQGIKKEPSCSWIEVDG 849 HSP 2 Score: 139.813 bits (351), Expect = 2.751e-30 Identity = 78/227 (34.36%), Postives = 125/227 (55.07%), Query Frame = -2
Query: 1672 FKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGK 2352
+K + ++S+L + ++K G +H H+IK GL+ ++ + SLI+ YAK G + A+ + + +RD+ SW+ALI G+ + F +QR+GV PN F LATCL +CS + G+ +H A+K G D+FV S++VD+YA C +E AE F M + VSWN +L GY + G KV+ F M D + + + VL + G++ G+
Sbjct: 92 YKKRLREYSSLLHDSAAKGSLKEGKLIHGHVIKCGLELDSHLWVSLINAYAKCGTVVYAQRVLNIMPQRDVVSWSALITGFVAQGYGSDGVCLFCAIQREGVRPNEFALATCLKACSMSHDVGFGKQVHLEAVKLGFFSDLFVGSALVDLYAKCSGMELAERVFKSMPELNVVSWNALLNGYAESGDGQKVMHLFCSMKDMEKKFSKFSLSTVLKGFANSGYIKAGQ 318
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: B9RKB2_RICCO (Pentatricopeptide repeat-containing protein, putative OS=Ricinus communis GN=RCOM_1048080 PE=4 SV=1) HSP 1 Score: 718.383 bits (1853), Expect = 0.000e+0 Identity = 365/634 (57.57%), Postives = 464/634 (73.19%), Query Frame = -2
Query: 1651 LKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLT 3552
LK YS +L +CASKG+L+ A+HG +IK G +PD HLW SL+N YAK G +A KVL M ERDVVSWTAL +G V+EG G D + YC M+K+ + PN L T LKA S+ D+ FG L+H E +K G+LLD+FVGS+LVDLYAK G+MELA+RVF M +KN VSWNALLNGYA +GD +L LF M E E+ + +TLS VLK C NL G HSL I+ E DEF+GC L+D+YSKCG+A + LKVF I+EP+IVAWSA+I LD+QG QEAAELF LMR KGV PNQ +FAS+ A N+GD + +H CI K+G+ES+ + NALI MYMK VQ+G RVF M++ + SWN+LLSG + +T D+G IF +ML EG P++ TF +LRSCS+LLN+ FG QVHAHIIKN L N+ VGT+LID+YAKN L DA++ F +L RDLF+WT +IAG++Q+D +EKA+K M R+G+ PN FTLA+CLS CS MA L NGQ LHS+AIK+G+SGDVFVSS++VDMY CGC+E+AEA F G+ S DTV+WN ++ GY QH QG K LE FRMMLD+ ++PDEVTFI VL+ACSYMG+V EGK++FD ++
Sbjct: 93 LKRYSGMLRECASKGNLNEGTAIHGNVIKSGLEPDSHLWVSLINLYAKCGSLAFARKVLVGMRERDVVSWTALIAGYVSEGCGSDGVKAYCEMRKENICPNEFTLATVLKASSMCSDIKFGKLIHLEAIKTGLLLDLFVGSALVDLYAKFGEMELADRVFFGMPEKNNVSWNALLNGYAQRGDGKNVLKLFCRMLECEMNFTNYTLSTVLKGCANSGNLREGKALHSLSIRRAYELDEFLGCNLVDMYSKCGMAYEALKVFNMIEEPDIVAWSAIITGLDQQGHSQEAAELFHLMRQKGVRPNQFSFASVISAATNVGDLYLGQSIHCCICKYGYESDNSVGNALITMYMKSGFVQDGIRVFDTMTNRDLVSWNALLSGFYDFETSDQGLRIFCQMLMEGLVPNLYTFVGVLRSCSSLLNVWFGKQVHAHIIKNSLDGNDFVGTALIDMYAKNRCLEDADVAFNKLTNRDLFTWTVIIAGHSQTDKAEKAVKYLGQMLREGIKPNEFTLASCLSGCSRMATLGNGQQLHSLAIKSGHSGDVFVSSALVDMYGKCGCMEDAEAIFKGLFSRDTVAWNTIICGYSQHGQGQKALEAFRMMLDEDIDPDEVTFIGVLAACSYMGWVEEGKKHFDLMS 726 HSP 2 Score: 119.013 bits (297), Expect = 8.004e-24 Identity = 73/241 (30.29%), Postives = 118/241 (48.96%), Query Frame = -2
Query: 2851 LEG-AKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGC 3570
+EG L + +L C+S ++ K VH IIK D + + +L++ YAK A +++ RD+ +WT + +G + M ++G+ PN L + L CS L G LH +K G DVFV S+LVD+Y KCG ME AE +F + ++ V+WN ++ GY+ G K L F+ M + ++ + T VL +C+Y+ + G L+ K+ C
Sbjct: 490 MEGLVPNLYTFVGVLRSCSSLLNVWFGKQVHAHIIKNSLDGNDFVGTALIDMYAKNRCLEDADVAFNKLTNRDLFTWTVIIAGHSQTDKAEKAVKYLGQMLREGIKPNEFTLASCLSGCSRMATLGNGQQLHSLAIKSGHSGDVFVSSALVDMYGKCGCMEDAEAIFKGLFSRDTVAWNTIICGYSQHGQGQKALEAFRMMLDEDIDPDEVTFIGVLAACSYMGWVEEGKKHFDLMSKSLC 730
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: A0A068U4K2_COFCA (Coffea canephora DH200=94 genomic scaffold, scaffold_9 OS=Coffea canephora GN=GSCOC_T00041696001 PE=4 SV=1) HSP 1 Score: 717.998 bits (1852), Expect = 0.000e+0 Identity = 371/772 (48.06%), Postives = 522/772 (67.62%), Query Frame = -2
Query: 922 ALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFE-SEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCS-EKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKHGEIYIKLKELVEKSILAGYVPKTDHILNDVSDDEKLEQLFHHNERLALAFALISINPVKTIRIFKNLHICEDCHDFMKLVSGVIKQEIVVRDANCFHHFKSGICSCQDY 3231
+ C+++L L G H +K+G+ D + SL++ YAKCG + A RVF M +K+VVSW AL++G+ +G + + LF M +R ++FTL+ VL++C+ + L + H+ V+K D +V L+DLY+KCG E KVF I E N V+W+ +++ + G + LF M + +T +++ A+ + VH+ K G + I+SNALI MYMK+ V +G +VF MS + SWN+LLSG +G+ D+GP IF KML EG +P+ T S LRSC++ LN FG QVHA+++KN L + VGT+LID+Y+K L D EL+F +L ERD+F+WT LIAGYAQ+D EKA+ FN MQR+GV N FTLA+CL +C+ +A L NGQ LHS AIK+G+ DV+V+S++VDMY CGC+++AE F + + DTV WN M+ G+ +H Q K L++F ML+K ++PD V+FI VLSACS+MG V EGK++F ++ ++GI P++ H ACM+DILGRAG+F+E+ESFI+ MK+ N LIW+TVLG C++HGN ++GE AA+KLFE+ P S YILL+NI+AAKGRW DV R+RALM+ GVKKEPGCSWV V+ ++H+F S D HP+ +IY KL++L ++ GY P T ++L++V D +K E LFHH+ERLALAFAL+S + IRIFKNL IC DCH+FMK VS + +EIV+RD+N FHHF +GICSC+DY
Sbjct: 166 TFRYCAVNLCLNEGKARHANLIKIGIDPDSHLFVSLINFYAKCGALSFARRVFDEMPEKDVVSWTALISGFVAEGLGREGVELFCEMRREGIRPNEFTLATVLRACSMVSGLEFAKQLHAEVVKWEAFTDVYVASALVDLYAKCGEMEYADKVFFVIPEQNAVSWNVLLNGYAQLGDGHKLLNLFCKMTESDTRFSNYTLSTVLKGCASSQSLRAGQVVHSMAIKIGSAFDDFIISNALITMYMKLGLVYDGLKVFSAMSQKDVVSWNALLSGYHDGENSDQGPMIFKKMLIEGLRPNQYTLISTLRSCTSQLNASFGKQVHAYLLKNNLCTDGHVGTALIDMYSKCRCLDDVELIFNRLSERDIFTWTILIAGYAQTDNQGEKALGFFNRMQREGVKANEFTLASCLRACAGIASLINGQQLHSWAIKSGHFCDVYVASALVDMYGKCGCVDDAEMIFKSIETVDTVLWNTMICGHSKHGQNEKALQSFGAMLNKDVQPDGVSFIGVLSACSHMGLVEEGKKHFHLMSELYGIAPSVDHYACMVDILGRAGRFSELESFIQHMKIAPNTLIWKTVLGACKIHGNVEMGEKAAQKLFEIEPDEDSSYILLSNIYAAKGRWNDVSRIRALMSSRGVKKEPGCSWVEVDAKMHVFLSQDASHPRLTDIYQKLEDLHQRLQSVGYTPNTHYVLHNVPDADKKENLFHHSERLALAFALVSNANSRRIRIFKNLRICGDCHEFMKGVSDITNKEIVIRDSNRFHHFHNGICSCKDY 937 HSP 2 Score: 373.629 bits (958), Expect = 1.609e-107 Identity = 223/625 (35.68%), Postives = 349/625 (55.84%), Query Frame = -2
Query: 1708 LEGAKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEF-VGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXX-XXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQL-LHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGM-VSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLS 3570
L+ + L+ YS+ CA L+ KA H +IK G DPD HL+ SL+N YAK G +A +V D MPE+DVVSWTAL SG V EG G + ++L+C M+++G+ PN L T L+ACS+ L F LH E VK DV+V S+LVDLYAKCG+ME A++VF + ++N VSWN LLNGYA GD K+LNLF MTES+ R S +TLS VLK C +L G + HS+ IK G D+F + L+ +Y K GL D LKVF + + ++V+W+A++ + + +F M +G+ PNQ+T S + + + + VHA + K+ ++ + ALI MY K R + + +F +S + + + +Q ++ F++M EG K + T S LR+C+ + ++ G Q+H+ IK+G + V ++L+D+Y K G + DAE++F + D W +I G+++ +EKA++ F M + V P+ + LS+CS+M ++E G+ H M+ G + V + +VD+ G E E+ M ++ +T+ W +L G + +++ E L + +EPDE + +LS
Sbjct: 154 LDRGRVLRWYSETFRYCAVNLCLNEGKARHANLIKIGIDPDSHLFVSLINFYAKCGALSFARRVFDEMPEKDVVSWTALISGFVAEGLGREGVELFCEMRREGIRPNEFTLATVLRACSMVSGLEFAKQLHAEVVKWEAFTDVYVASALVDLYAKCGEMEYADKVFFVIPEQNAVSWNVLLNGYAQLGDGHKLLNLFCKMTESDTRFSNYTLSTVLKGCASSQSLRAGQVVHSMAIKIGSAFDDFIISNALITMYMKLGLVYDGLKVFSAMSQKDVVSWNALLSGYHDGENSDQGPMIFKKMLIEGLRPNQYTLISTLRSCTSQLNASFGKQVHAYLLKNNLCTDGHVGTALIDMYSKCRCLDDVELIFNRLSERDIFTWTILIAGYAQTDNQGEKALGFFNRMQREGVKANEFTLASCLRACAGIASLINGQQLHSWAIKSGHFCDVYVASALVDMYGKCGCVDDAEMIFKSIETVDTVLWNTMICGHSKHGQNEKALQSFGAMLNKDVQPDGVSFIGVLSACSHMGLVEEGKKHFHLMSELYGIAPSVDHYACMVDILGRAGRFSELESFIQHMKIAPNTLIWKTVL-GACKIHGNVEMGEKAAQKLFE-IEPDEDSSYILLS 776 HSP 3 Score: 144.436 bits (363), Expect = 1.099e-31 Identity = 77/211 (36.49%), Postives = 122/211 (57.82%), Query Frame = -2
Query: 1699 FTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACS 2331
++ R C+ L + G HA++IK G+ ++ + SLI+ YAK G L+ A +F ++ E+D+ SWTALI+G+ + ++ F M+R+G+ PN FTLAT L +CS ++ LE + LH+ +K DV+V+S++VD+YA CG +E A+ F + + VSWN++L GY Q G K+L F M + T VL C+
Sbjct: 163 YSETFRYCAVNLCLNEGKARHANLIKIGIDPDSHLFVSLINFYAKCGALSFARRVFDEMPEKDVVSWTALISGFVAEGLGREGVELFCEMRREGIRPNEFTLATVLRACSMVSGLEFAKQLHAEVVKWEAFTDVYVASALVDLYAKCGEMEYADKVFFVIPEQNAVSWNVLLNGYAQLGDGHKLLNLFCKMTESDTRFSNYTLSTVLKGCA 373
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Match: I1HP48_BRADI (Uncharacterized protein OS=Brachypodium distachyon GN=BRADI2G42710 PE=4 SV=1) HSP 1 Score: 670.618 bits (1729), Expect = 0.000e+0 Identity = 342/771 (44.36%), Postives = 493/771 (63.94%), Query Frame = -2
Query: 1249 AKRLKLYSKILHDCASKGSLSSAKAVHGKIIKKGFDPDLHLWNSLVNAYAKRGVFGYACKVLDRMPERDVVSWTALFSGLVNEGYGYDVLDLYCSMKKDGVLPNGHCLVTALKACSLSLDLFFGTLLHGEGVKVGVLLDVFVGSSLVDLYAKCGQMELAERVFLFMDKKNVVSWNALLNGYALKGDAGKILNLFQGMTESELRCSKFTLSNVLKSCTYLVNLTWGLIAHSLVIKTGCEHDEFVGCCLLDLYSKCGLAEDTLKVFKRIQEPNIVAWSAMIDCLDEQGQIQEAAELFSLMRCKGVSPNQHTFASIAGVAANLGDRLYCEGVHACIFKHGFESEIILSNALIAMYMKIRSVQNGWRVFKEMXXXXXXXXXXXXXXSQNGKTCDRGPSIFHKMLAEGFKPDICTFTSILRSCSNLLNIKFGHQVHAHIIKNGLKDNNLVGTSLIDLYAKNGFLADAELLFTQLIERDLFSWTALIAGYAQSDCSEKAIKCFNHMQRQGVTPNNFTLATCLSSCSNMAMLENGQLLHSMAIKAGNSGDVFVSSSIVDMYANCGCIEEAEAAFLGMVSTDTVSWNMMLFGYLQHRQGLKVLETFRMMLDKGLEPDEVTFIAVLSACSYMGFVNEGKEYFDSLTNVFGIVPTIKHCACMIDILGRAGKFNEVESFIKDMKVTSNPLIWETVLGTCRMHGNDKLGESAAEKLFELNPGIASHYILLANIFAAKGRWEDVRRVRALMTHCGVKKEPGCSWVMVNGQVHIFRSTDGCHPKH 3561
A+ L+L++ L DCA + +L + +H ++++ PD L +SL+N Y K G A +V D MP RD+V+WTA+ S G LD++ M ++G+ PNG L + LKACS F +HG+ VK+ L D +VGSSLV+ Y CG+++ AE V L + +++ VSWNALLNGYA GD +++ + + + S SK+TL VLK C L +G H+ VIK G E D + CL+++YS+C AE+ +VF RI EP++V SAMI C D EA +LF M GV PN + F IAGVA+ GD C VHA I K GF + +A++ MY+K+ +VQ+ F + ++ SWN++LS +G C++G IF +M EGF + T+ S+LR C++L+N++FG QVHA I+K+GL+++ V L+D+YA++G A L+F QL ERD FSWT +++GYA+++ +EK ++ F M R+ + P++ TLA LS CS+MA L +G LHS AIK+G + V VS ++VDMY CG I +AE F + D V+WN ++ GY QH G K L+ F+ M+D+G PD +TF+ VLSACS+ G +NEG++YF SL++++GI PT++H ACM+DIL +AG+ E ES I M + + IW T+LG CR+H N ++ E AAE+LFEL P AS ILL+NI+A GRW DV RVR ++ GVKKEPGCSW+ +NGQ+H+F S DGC PK+
Sbjct: 47 AEELRLHAAALQDCAVRRTLRRGQELHARLLRSALHPDTFLLDSLLNMYCKCGRLVDARRVFDGMPHRDIVAWTAMISAHTAAGDSDQALDMFARMNQEGIAPNGFTLASVLKACSGGSHSKFTHQVHGQVVKLNGLDDPYVGSSLVEAYTSCGELDAAETVLLGLPERSDVSWNALLNGYARHGDYRRVMIIIEKLVASGDEISKYTLPTVLKCCMELGLAKYGQSVHASVIKRGLETDNVLNSCLVEMYSRCLSAEEAYEVFIRIDEPDVVHCSAMISCFDRHDMAWEALDLFVKMSGMGVKPNHYIFVGIAGVASRTGDANLCRSVHAYIVKSGFAMLKGVGDAILNMYVKVGAVQDATVTFDLIHEPDTFSWNTILSAFYSGSNCEQGLRIFKQMACEGFSANKYTYVSVLRCCTSLMNLRFGTQVHACILKSGLQNDTDVSRMLVDMYAQSGCFTSACLVFEQLKERDAFSWTVIMSGYAKTEEAEKVVEYFRSMLRENIRPSDATLAVSLSVCSDMASLGSGLQLHSWAIKSGWNSSV-VSGALVDMYVKCGNIADAEMLFHESETRDQVAWNTIICGYSQHGHGYKALDAFKQMVDEGKRPDGITFVGVLSACSHAGLLNEGRKYFKSLSSIYGITPTMEHYACMVDILSKAGRLVEAESLINQMPLAPDSSIWRTILGACRIHRNIEIAERAAERLFELEPHDASSSILLSNIYADLGRWSDVTRVRNILLDHGVKKEPGCSWIEINGQIHMFLSQDGC-PKY 815 The following BLAST results are available for this feature:
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy Swiss-Prot
Analysis Date: 2016-11-01 (Homology Analysis for Gossypium barbadense CottonGen RefTrans V1 vs Swissprot) Total hits: 25
Pagesback to top
BLAST of g.barbadense_cottongen_reftransV1_0091917 vs. ExPASy TrEMBL
Analysis Date: 2016-11-02 (Homology Analysis for Gossypium barbadense CottonGen RefTrans V1 vs TrEMBL) Total hits: 25
Pagesback to topInterPro
Analysis Name: InterProScan analysis for Gossypium barbadense CottonGen RefTrans V1
Date Performed: 2016-11-02
Sequences
The
following sequences are available for this feature:
contig sequence >g.barbadense_cottongen_reftransV1_0091917 ID=g.barbadense_cottongen_reftransV1_0091917; Name=g.barbadense_cottongen_reftransV1_0091917; organism=Gossypium barbadense; type=contig; length=3811bpback to top |