g.barbadense_cottongen_reftransV1_0095004, g.barbadense_cottongen_reftransV1_0095004 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy Swiss-Prot
Match: SMG1_MOUSE (Serine/threonine-protein kinase SMG1 OS=Mus musculus GN=Smg1 PE=1 SV=3)

HSP 1 Score: 493.041 bits (1268), Expect = 5.484e-138
Identity = 294/767 (38.33%), Postives = 427/767 (55.67%), Query Frame = 2

Query: 5147 LIDIWWSLRRRRVSLFGHAAHAFIKYLLYSSTKL----SDGRLSGDFCQSQKQKVGSYTLRATLYVMHVLLNYGLELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLVDI-----------NAY---------------------------------EEKPSEELQHILGCLRELY-----------PRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKPETPHELWFHHEYKEQLKSAIVSLKTPPASAAALGDVWRPFDHIAASLASYQRKSS---ISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKSSVPP------VPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKGQRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEWTRGDFHDDAAI----GGEE------RKGMELAVSLSLFASRVQEIRV 7213
            +I +W  +  R  SL+  +  A+  +L  ++ ++     D RL        +Q      + ATL ++ +L+ +  EL+  LE  L T P   W+ + PQLF+RL+ HPE  VR+ I +LL  +A+ SP  I+YP +V             N Y                                 +++P   L      +++ Y           P ++  VQ+++ EL  VTVLW+ELWL  L   HM V+RRI  L++E  R+  N TL + EK  I   K++A+M PIV ALE   + T+   ETPHE WF   Y + + +A+  LKTP ++ A  G  W PF  I  SL    +K +   + L E++P LA ++++++ +PG   +V+  ++           VTI S    +TIL TKTKPKKL+ LGSDGK+Y YL KG EDL LD RIMQ L  +N+   + +         R+YSVTP+  R+GLIQWVD    ++ ++K WQ R      +AL A  A+ S         VPRPS+++Y KI PALK  G+   +SRRDWP  V + VL +LM+  P  LL +ELW +      +    + Y+ S A MSMVG+I+GLGDRHLDN+L+D ++G+VVHIDYNVCF+KG+ L+VPE VPFR+TQ IE ALG+TG+EG FR +CE V+ ++R+ ++ LL LLE FV+DPL++WT G     A      GG++      ++ ME  ++ SLF+SRV EI+V
Sbjct: 1727 VIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQVLLDEDDPRLH--LSHRAEQSTDDVIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLN-HPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKYSSAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIDNALEKLKTP-SNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLDEISPWLAAMTNTEIALPG---EVSARDT-----------VTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQRE-----AALQAQKAQDSYQTPQNPSIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKV 2470          

HSP 2 Score: 88.5817 bits (218), Expect = 1.446e-15
Identity = 116/502 (23.11%), Postives = 207/502 (41.24%), Query Frame = 2

Query: 2510 LHSQQLVSILSYI--SQRWKVPLSSWIQRLIHTCRSSKDSSLGQLEETGIVGANDLWLDIKMENILEKFCSVNNLAGAWWAIHEAARYCISTRLRTNLGGPTQTFAALERMLLDV-SHVLQLDGEQNDGSLSIIGSS-GAHLLPMRLLLDFVEALKKNVYNAYEGSA-VLPSAGRQSSLFFRANKKVCEEWFSRICEPMMNAGLALQCHDATIQYCTLRLQELKNLVVSAFKEKSQAQVTEYLHNMREKYIGDISRIVRHMSLALCRNHESEALIGLQKWVSLTFSPLLLDEDQSVNHSGIVEPFQWITGLVYQAEGQYEKA-VSHFAHLLQT----------EESLSTMGSDGAQ-----------------------------FCIARIIESYTAVSDWKSLESWLLELQTLRAKYAGKSYSGALTTAGNEMNAIHALAQFDEGDLQAAWAYLDLTPKSSSELTLDPKLALQRSEQMLLQALLFKIEGNLDKVPHELQKA 3880
             H Q    ++S+I      +    +W++RL ++C+                      LD + ++ + +     +     WAI EAA++ + ++LRT LG    TF  +E ++  + +H L  D + +  + +      G++ L + LLL ++E L+K +YNAYEG A  L S  +    FF  N++ C++W +RI   +M  GL       T+++    L E+K    ++  + S+ +VT             I  +V     ALC  H  EA+ G+  W S                S + +   WI  +  QAEG++EKA V +  HL             ++S+ T+ + G                               +   +  E Y +++DW +++ W   +  L+     K+ S        + N I +L+ F+ G+       L+L P  +  L     LA    E++ ++ LL  +   L   P ELQK+
Sbjct:  855 FHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQR---------------------LDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGSNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMK---TNSLTQGSELEVT-------------IMMVVE----ALCELHCPEAIQGIAVWSS----------------SAVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKSIDSSPEVISYLGNKACECYISIADWAAVQEWQNAVHDLK-----KNSSSTSLNLKADFNYIKSLSSFESGEFVECTEQLELLPGENINL-----LAGGSKEKIDMKKLLPNM---LSPDPRELQKS 1286          

HSP 3 Score: 82.4185 bits (202), Expect = 1.206e-13
Identity = 33/57 (57.89%), Postives = 48/57 (84.21%), Query Frame = 3

Query: 11706 KNAYAMSVLRRVEMKLDGRDITERREISIAEQVDYLIKQATSVDNLCSMYEGWTPWI 11876
            +N+YA+SV +RV+ KL+GRD+   R +S+AEQVDY+IK+AT++DNL  +YEGWT W+
Sbjct: 3602 RNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV 3658          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy Swiss-Prot
Match: SMG1_HUMAN (Serine/threonine-protein kinase SMG1 OS=Homo sapiens GN=SMG1 PE=1 SV=3)

HSP 1 Score: 490.73 bits (1262), Expect = 2.885e-137
Identity = 293/772 (37.95%), Postives = 428/772 (55.44%), Query Frame = 2

Query: 5141 DKLIDIWWSLRRRRVSLFGHAAHAFIKYLLYSSTKLSDGRLSGD-------FCQSQKQKVGSYTLRATLYVMHVLLNYGLELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLVDI--------------------------------------------NAYEEKPSEELQHILGCLRELY-----------PRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKPETPHELWFHHEYKEQLKSAIVSLKTPPASAAALGDVWRPFDHIAASLASYQRKSS---ISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKSSVPP------VPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKGQRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEWTRGDFHDDAAI----GGEE------RKGMELAVSLSLFASRVQEIRV 7213
            + +I +W  +  R  SL+  +  A+  +L     KL+ G++  D            +Q      + ATL ++ +L+ +  EL+  LE  L T P   W+ + PQLF+RL+ HPE  VR+ I +LL  +A+ SP  I+YP +V                                              ++ +++P   L      +++ Y           P ++  VQ+++ EL  VTVLW+ELWL  L   HM V+RRI  L++E  R+  N TL + EK  I   K++A+M PIV ALE   + T+   ETPHE WF   Y + +++A+  LKTP  + A  G  W PF  I  SL    +K +   + L E++P LA ++++++ +PG   +V+  ++           VTI S    +TIL TKTKPKKL+ LGSDGK+Y YL KG EDL LD RIMQ L  +N+   + +         R+YSVTP+  R+GLIQWVD    ++ ++K WQ R      +AL A  A+ S         VPRPS+++Y KI PALK  G+   +SRRDWP  V + VL +LM+  P  LL +ELW +      +    + Y+ S A MSMVG+I+GLGDRHLDN+L+D ++G+VVHIDYNVCF+KG+ L+VPE VPFR+TQ IE ALG+TG+EG FR +CE V+ ++R+ ++ LL LLE FV+DPL++WT G     A      GG++      ++ ME  ++ SLF+SRV EI+V
Sbjct: 1727 EGVIKVWRKVVDRIFSLYKLSCSAYFTFL-----KLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLN-HPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTP-LNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPG---EVSARDT-----------VTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQRE-----AALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKV 2472          

HSP 2 Score: 87.0409 bits (214), Expect = 4.227e-15
Identity = 117/502 (23.31%), Postives = 204/502 (40.64%), Query Frame = 2

Query: 2510 LHSQQLVSILSYI--SQRWKVPLSSWIQRLIHTCRSSKDSSLGQLEETGIVGANDLWLDIKMENILEKFCSVNNLAGAWWAIHEAARYCISTRLRTNLGGPTQTFAALERMLLDV-SHVLQLDGEQNDGSLSIIGSS-GAHLLPMRLLLDFVEALKKNVYNAYEGSA-VLPSAGRQSSLFFRANKKVCEEWFSRICEPMMNAGLALQCHDATIQYCTLRLQELKNLVVSAFKEKSQAQVTEYLHNMREKYIGDISRIVRHMSLALCRNHESEALIGLQKWVSLTFSPLLLDEDQSVNHSGIVEPFQWITGLVYQAEGQYEKA-VSHFAHLLQT----------EESLSTMGSDGA-----------------------------QFCIARIIESYTAVSDWKSLESWLLELQTLRAKYAGKSYSGALTTAGNEMNAIHALAQFDEGDLQAAWAYLDLTPKSSSELTLDPKLALQRSEQMLLQALLFKIEGNLDKVPHELQKA 3880
             H Q    ++S+I      +    +W++RL ++C+                      LD + ++ + +     +     WAI EAA++ + ++LRT LG    TF  +E ++  + +H L  D + +  + +      G + L + LLL ++E L+K +YNAYEG A  L S  +    FF  N++ C++W +RI   +M  GL       T+++    L E+K    ++  + ++ +VT             I  +V     ALC  H  EA+ G+  W S      LL                WI  +  QAEG++EKA V +  HL             ++S+ T+ + G                               +   +  E Y +++DW +++ W   +  L+     KS S        + N I +L+ F+ G        L+L P  +  L     LA    E++ ++ LL  +   L   P ELQK+
Sbjct:  857 FHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQR---------------------LDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMK---TTSLSQGNELEVT-------------IMMVVE----ALCELHCPEAIQGIAVWSSSIVGKNLL----------------WINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLK-----KSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINL-----LAGGSKEKIDMKKLLPNM---LSPDPRELQKS 1288          

HSP 3 Score: 82.4185 bits (202), Expect = 1.257e-13
Identity = 33/57 (57.89%), Postives = 48/57 (84.21%), Query Frame = 3

Query: 11706 KNAYAMSVLRRVEMKLDGRDITERREISIAEQVDYLIKQATSVDNLCSMYEGWTPWI 11876
            +N+YA+SV +RV+ KL+GRD+   R +S+AEQVDY+IK+AT++DNL  +YEGWT W+
Sbjct: 3605 RNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV 3661          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy Swiss-Prot
Match: SMG1_DROME (Serine/threonine-protein kinase Smg1 OS=Drosophila melanogaster GN=nonC PE=1 SV=2)

HSP 1 Score: 384.03 bits (985), Expect = 5.634e-105
Identity = 229/677 (33.83%), Postives = 372/677 (54.95%), Query Frame = 2

Query: 5135 VIDKLIDIWWSLRRRRVSLFGHAAHAFIKYLLYSSTKLSDGRLSGDFCQSQKQKV---GSYTLRATLYVMHVLLNYGLELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLVDINAYEE---------KPSEE----LQHILGCLRELYPRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKPETPHELWFHHEYKEQLKSAIVSLKTPPASAAALGDVWRPFDHIAASLASYQRKSSISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHS-SSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKSSVPPVPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKGQRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEWTRGD 7114
             +D ++ IW          +  AA ++ +YL + S    + +  G+   SQ++++    S  +  TL ++ +++ +   L++ LE  L T P+  W+ + PQLF+RL+ H E  VRK +  LL  LAK  P  +++P +V  N  ++         +P+ E      ++LG L +  P  +Q V+L++ EL  V +LW+E W+ +L  ++   + R++ L           T  + + ++    +++     ++  LE  +A TSR PET +E  F   +   ++  + +L+      A   D  +   HI  S       S++ +  ++P L  +    + MPGL+    P + D+         V I S    + +L TKTKPKK+   GS+G+ YT+L KG EDL LD RIMQ L   N+ +   S A  +      +YSV P+  ++GLI WVD V  +++++K WQ R   +QV+      A ++VP   R +D+FY K+ P L +  ++    RR WP  V  +VL +L +E P  LL +ELWC +     +   ++R+   ++ MSM+G+++GLGDRHLDN+L++  SGD+VHIDYNVCF+KG+ L++PE VPFRLTQ +  A+G+TGIEG FR  CE V+ V+RK ++ LL LLE FV+DPL++WT  D
Sbjct: 1551 ALDAILQIWRRAIANTYDYYKDAARSYFQYLSFKSGSGPE-KPEGEGVVSQRERLHVDDSNLVTTTLRLLRLIVKHASGLQEVLEQGLHTTPIAPWKVIIPQLFSRLNHH-EPYVRKSVCDLLCRLAKSRPQLVIFPAVVGANREQQDATAPPATARPTTEDACCYGYLLGELSKQAPEAVQHVKLMVKELRRVCLLWDEYWIHSLAHIYNTYVSRVSAL----------ATDFRPDDHEGKNNRFNVWRPQLLADLEALVAVTSRPPETTYERSFRKRFDAPIRLTVDALRHRRYPEA--WDKLKQLYHILQSNMIRGSGSTLKMQSISPVLCGIGRMRISMPGLDAH-GP-DGDQ---------VYIESVESSVCVLPTKTKPKKVAFYGSNGQRYTFLFKGMEDLHLDERIMQFLSISNAIMACRSDAPGNGCYRAHHYSVIPLGPQSGLISWVDGVTPVFALYKKWQQR--RSQVAGNAGAGAVANVPR--RFTDLFYNKLSPLLAKHNMQVSDPRRQWPISVLLQVLDELSQETPNDLLARELWCQAGNAAEWRQSVRRFVRCMSVMSMIGYVIGLGDRHLDNVLINLGSGDIVHIDYNVCFEKGRTLRIPEKVPFRLTQNLVQAMGITGIEGPFRLGCEYVLKVMRKERETLLTLLEAFVYDPLVDWTTND 2198          

HSP 2 Score: 93.9745 bits (232), Expect = 3.475e-17
Identity = 145/580 (25.00%), Postives = 246/580 (42.41%), Query Frame = 2

Query: 2606 RSSKDSSLGQLEETGIVGANDLWLDIKMENILEKFCSVNNLAGAWWAIHEAARYCISTRLRTNLGGPTQTFAALERMLLDVSHVLQLDG-----EQNDGSLSIIGSSGAHLLPMRLLLDFVEALKKNVYNAYEGSA-VLPSAGRQSSLFFRANKKVCEEWFSRICEPMMNAGLALQCHDATIQYCTLRLQELKNLVVSAFKEKSQAQVTEYLHNMREKYIGDISRIVRHMSLALCRNHESEALIGLQKWVSLTFSPLLLDEDQSVNHSGIVEPFQWITGLVYQAEGQYEKAVSHFAHLLQTEESLSTMGSDGAQFCIARIIESYTAVSDWKSLESWLLEL-QTLRAKYAGKSYSGALTTAGNEMNAIHA-LAQFDEG-----DLQAAWAYLDLTPK------SSSELTLDPKLA----LQRSEQMLLQALLFKIEGNLDK-VPHELQKAKSMLEEMLSVLPLDGLAEATAC--VTQL-HCIFAVGEGYDL-----SQGNCWKHMGSQGKSKLNQSGLGSYLLPLQPLIKGIHQDCNPWLKVLRVYRTISPTSPVTLKLSMNLMSLARKQGNLTLANCLIN 4249
            +  +DS +  L E  +        D +  N+ +       LA AW    EAARYC+  RLRT +G P +TF   E +++   H   L G     E++      +    +    + LLL F++AL+K +YNA EGSA  L    +Q + FFR N   C+ WF+RI   ++   + +Q  +  I+Y        + ++V+     S+ Q   Y            S+ + +M+ +L    E+++L GL+ W                      + ++W+     QA G+ E A++ +  +L  +E  S +     QF ++++++    +  W    S L+EL Q    +   +  +  L  +  E+NA+   LA+ +E       L   +  L L P       SSS L+     +     QR+E ++L  LL       D+ VP +   AK++L+       L+   +  +C  +T L H +  V  G +L     S G C      Q +SK   S               I   C  W ++LR +   +P S  T  L ++  + AR++GNL LA  L+ 
Sbjct:  809 KPEQDSLIHCLRELFVRSCQVAPQDERQMNLSQCTKRCQRLAIAWLQF-EAARYCVDQRLRTTVGKPQETFLGFEAIIM--RHARLLSGCAKEIERSALDDLSLEELLSMQSNLSLLLGFLDALEKLIYNAAEGSAFALRPPEKQVAAFFRLNNPTCQSWFNRIRIGVVIIAMHVQQPELVIRYA-------QQILVN-----SKTQDPTY------------SQAIVYMAWSLVSCQEADSLRGLRLWA----------------RGKSCKSYKWLKYAADQAAGKRESALAGYRTILAEKELQSELEPHTRQFVVSQMMQCLQDLGQW----SQLVELKQQQMTRPEDRELNPFLQRSNVEVNALERLLAKSEESCSSMDALGGVFQQLSLWPSNWDESVSSSGLSERASFSSIHMRQRTEDIVLHKLLE------DRCVPDQ---AKNLLDTQWRDSLLNPSFDQRSCKELTLLRHIVQGVSGGQELSLLPVSSGRC------QNRSKFISS--------------AILMRCLAWTQLLRQH--CAPGSWET--LCLDAAAAAREEGNLQLAETLLT 1308          

HSP 3 Score: 73.559 bits (179), Expect = 6.059e-11
Identity = 30/76 (39.47%), Postives = 53/76 (69.74%), Query Frame = 3

Query: 11649 ESHEVPVPSTDTASRIARGKNAYAMSVLRRVEMKLDGRDITERREISIAEQVDYLIKQATSVDNLCSMYEGWTPWI 11876
            +S+E+   +  + +   + +NAY +SV +++ MKL+GRD    +  ++AEQVDY+I++A + +NL  +YEGWTPW+
Sbjct: 3143 DSYEIFTHAKGSGNVHEQKRNAYGVSVWKKIRMKLEGRDPDSNQRSTVAEQVDYVIREACNPENLAVLYEGWTPWV 3218          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy Swiss-Prot
Match: SMG1_CAEBR (Serine/threonine-protein kinase smg-1 OS=Caenorhabditis briggsae GN=smg-1 PE=3 SV=3)

HSP 1 Score: 374.015 bits (959), Expect = 3.041e-102
Identity = 231/672 (34.38%), Postives = 370/672 (55.06%), Query Frame = 2

Query: 5261 LSGDFCQSQKQKVGSYTLRATLYVMHVLLNYGLELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPML------VDINAYEEKPSEELQHILGCLREL-------YPRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLAST-SRKPETPHELWFHHEYKEQLKSAIVSLK-----TPPASAAALGDVWRPFDHIAASLASYQRKS---SISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIR--YYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKS--SVPPVPRPSDMFYGKIIPALKEKGIRRVIS--RRDWPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKGQRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEWTRGDFHDDAAIGGEERKGMELAVSLSLFAS 7192
            +SGDF      K    TL ATL ++ +L+ +G  L D +   LS   +  W+++ PQLFARLS HP + +RK +  L+  +   +P ++V+ ++       +++  EE+ +++   +  C  +L       YP L++DV+  + EL  + +L EE W   L  +  ++ +R+ ++K E A+   +  L   +K++I + K   +   I   L+     T   +PET +E  F + + E L  A    K     +P AS       W PF ++ ++ A    K    +    +++  LA L  S VPMPG E      E DR        +V+IA  ++++TIL TKT+PKKL  +GSDGK   +L KGREDL LD R+MQ L+  N  L S    +  +   +  +Y+V P+  R+GLI+WV+    I+ I++ WQ + +     AL   + K+  +VP + +P++M++  I  A     I  +I+  R  WP ++  +V   L  + P  L+ +E+W  +    A+ +  KRY+ S+A MSMVG +LGLGDRHLDN+L+D   G VVHIDYN+CFDKG+ L++PE VPFRL++ +  ALG + + GTFR +C  V+S LR    +L MLL+ FV+DPL++WT    HD+ +  G    G+ LA+ L+++ S
Sbjct: 1432 MSGDFECLPYSKREETTL-ATLRILEMLVKHGEVLVDVINDGLSRTNVHVWKEILPQLFARLS-HPSDHIRKTLVDLISRVCTAAPHAVVFQVVSGAASSTEVSDLEEQQNDDRNRVRACCEQLETKMAQSYPNLVRDVRQFVAELERINLLNEEKWSVVLGTMEHEMEKRLALIKAENAKTDFSMHLMPKQKDEIISKKTKLLTRQIFDVLDELYEKTIVAQPETENEKEFFNTFSEMLTKAHTESKNNRYNSPEAS-------WAPFKNLVSNFAHRTNKKGMQTFQTADISQYLATLGKSCVPMPGQES----VEFDR--------VVSIARVADNVTILPTKTRPKKLGFIGSDGKQLAFLFKGREDLHLDERVMQFLRLCNVMLQSEKGKSRQIAEYQAHHYAVIPLGPRSGLIKWVEGATPIFHIYRKWQMKEK-----ALKQATKKNGETVPEIEKPTNMYHNMIRQAFTAHNIDAIIASDRSKWPAQILEEVFDGLCSKTPTDLISREIWMRANDSTAWWAVTKRYARSLAVMSMVGSVLGLGDRHLDNLLVDLKYGHVVHIDYNICFDKGKILRIPETVPFRLSRNMRHALGPSDMYGTFRESCVHVLSTLRSGHQVLTMLLDAFVFDPLVDWTS---HDNISTSG----GVSLALQLAVYGS 2070          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy Swiss-Prot
Match: SMG1_CAEEL (Serine/threonine-protein kinase smg-1 OS=Caenorhabditis elegans GN=smg-1 PE=1 SV=3)

HSP 1 Score: 371.703 bits (953), Expect = 1.839e-101
Identity = 236/711 (33.19%), Postives = 372/711 (52.32%), Query Frame = 2

Query: 5147 LIDIWWSLRRRRVSLFGHAAHAFIKYLLYSSTKLSDGRLSGDFCQSQKQKVGSYTLRATLYVMHVLLNYGLELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPML-------VDINAYEEKPSEELQHILGCLREL-------YPRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTS-RKPETPHELWFHHEYKEQLKSAI----VSLKTPPASAAALGDVWRPFDHIAASLASYQRKSSISLGE---VAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGI---RYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKS--SVPPVPRPSDMFYGKIIPALKEKGIRRVIS--RRDWPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKGQRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEWTRGDFHDDAAIGGEERKGMELAVSLSLFAS 7192
             + IW  +R  R      A  ++ +++           +SGD C +        T  ATL ++ +L+ +G  L D +   L+   +  W+++ PQLFARLS HP E +RK +  L+  +   +P ++V+ ++        D    EE+ +++   +  C  +L       YP L++DV+  + EL  + +L EE W   +  +  ++ +R+++++ E A+      L+ S KN I   +   +   I   L+     T    P++ +E  F   + E L +A     +S  T P  +      W PF ++ A+      K  +   E   ++P LA LS+S VPMPG E      E DR        +V+I+  +  +TIL TKT+PKKL  +GSDGK   +L KGREDL LD R+MQ L+  N  L      +   +      +Y+V P+  R+GLI+WV+    ++ I++ WQ + +     AL   + K+  +VP + RPS+M++  I  A  +  I   I+  R  WP E+  +V   L  + P  L+ +ELW  +     + S  KRYS S+A MSMVG +LGLGDRHLDN+L+D   G VVHIDYN+CFDKG+ L++PE VPFRLT+ +  ALG + + GTFR +C  V+S LR    +L MLL+ FV+DPL++WT    H+  A       G+ LA+ L+++ S
Sbjct: 1410 FMHIWKMVRDHRTKFLSIAVTSYFQFI---------QNMSGD-CDNLPYSKKEETTLATLRILELLVKHGDVLIDVINDGLNKTNVHIWKEILPQLFARLS-HPSEHIRKTLVDLISKICTAAPHAVVFQVVSGAASSSTDGEELEEQQNDDRNRVRACCEKLETNMSQSYPNLVKDVRQFVAELERINLLNEEKWSVVMGTMEHEMEKRLSLIRTENAKTESALHLTASVKNDIIVKRTQLLTRQIFDVLDELYQQTVIEPPKSKNEEEFVTAFAEVLTNAFQESRISRTTSPEKS------WIPFKNLIANFVHRNSKKGMQTFETEDISPYLASLSNSCVPMPGQE----SVEFDR--------VVSISRVARQVTILPTKTRPKKLGFVGSDGKQVAFLFKGREDLHLDERVMQFLRLCNVMLQPGKGKHRQSVAAYQAHHYAVIPLGPRSGLIKWVEGATPMFHIYRKWQMKEK-----ALKQATKKNGETVPEIERPSNMYHNMIRLAFADHKIDSSITSDRSKWPAEILEEVFESLTAKTPTDLISRELWMRANDATTWWSVTKRYSRSLAVMSMVGSVLGLGDRHLDNLLVDLKWGHVVHIDYNICFDKGKNLRIPETVPFRLTRNMRHALGPSEMYGTFRESCVHVLSTLRSGHQVLTMLLDAFVFDPLVDWTS---HEHTATS-----GVSLALQLAVYGS 2078          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy Swiss-Prot
Match: SMG1_DICDI (Probable serine/threonine-protein kinase smg1 OS=Dictyostelium discoideum GN=smg1 PE=3 SV=1)

HSP 1 Score: 355.91 bits (912), Expect = 1.006e-96
Identity = 245/753 (32.54%), Postives = 370/753 (49.14%), Query Frame = 2

Query: 5168 LRRRRVSLFGHAAHAFIKYLLYSSTKLSDGRLSGDFCQSQKQKVGSYTLRATLYVMHVLLNYGLELKDTLEPAL----STVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLV-------------------------DINAYEEKPSEELQHILGCLRELYPR---LIQDVQLVINELGNVTVLWEELWLSTLQDL----HMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKP-ETPHELWFHHEYKEQLKSAIVSL--KTPPASAAALGDVWRPFDHIAASLASYQ--RKSSISLGEVAPQLAMLSSSDVPMPGLE-------------------KQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGA-----------------------------------------GSAKSSV---PPVPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKGQRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEWTRGD 7114
            L+    +    A  A+ ++L YS   + DG  +G              +RATL ++++L+  G +L +T E  L    ST P   +  + PQLFARLS HP+  V+K +  +L  + + +P+ IVY  +V                         DI   +++    LQ IL     L      L+++ + +I +LG +T LW++ W   ++ +    +++  +  +  ++  A I   T L  + K      K   ++ PI   L+R  A+T     +TPHE WF   + E +   I +   +  P S         PFD +   +A +Q  R  S+SL  V P LA+   +   MPG +                          S  +    +   VTI      + +L TKTKPKK+ +LGSDG  Y YLLKGREDL LD RIMQLL  ++  L +       LL  R YSV P+S  +GLIQWV+  + ++SI+K+W    Q+ +                                                +  S++   P + RP D+FY KI P L++ G+  +  R +WP E+  +VL +LM+E PK +L +ELW +S        K + YS S+A MS++G+++GLGDRHLDNILLD  +G++VHIDYN+CF+KG  LK+PE VPFR+TQ  E ALGLTG++GTFR     ++ +LRKNKD+LL LLE F++DPL +W   +
Sbjct: 1437 LKNTNTNELKTAVEAYFQFLKYS---MLDGNSNGGL-----------NIRATLKILNILVCSGNKLVETFERCLNELTSTRP---FTIIIPQLFARLS-HPDTFVQKYVVEILNRIGRDNPNKIVYQTIVGSLNFTNTNNNNNSSCAAATTDDGLDIELLQKQYPNYLQ-ILNIKENLLKHSELLVKETETLIYQLGKLTTLWDDNWQYFIEQIQGWVYINTKQWNDDYQQLKATIKNPTILKHTLK-----KKNQELLQPIYEKLKRLTAATVLSVCKTPHEKWFTKCHFETINKTIRAFEKQNKPTS---------PFDVLHDLIAEFQQYRLISLSLSSVNPSLALFRPTITQMPGTDLNYFNINHIHHHHHHHNHHGNNNNQHSTSSGNLPIQNQVTIQLIKPTIYLLPTKTKPKKMAMLGSDGNLYYYLLKGREDLHLDERIMQLLNVVDQLLMNDKKPTLKLLRTRNYSVIPLSQSSGLIQWVEGAVPLFSIYKNWYKNDQVYKQQQQQQQQLQQQQQQQQQQQQQQPQPQQQPQQQPQQQPQQQPQPQQNSTTTSNIVNKPIIARPVDIFYAKITPLLEKAGLNFMTPRSEWPKEILIQVLNELMQETPKWILQRELWFSSSSSSELFLKTQSYSRSLALMSVIGYMIGLGDRHLDNILLDLKTGEIVHIDYNICFEKGAELKIPERVPFRMTQIFEYALGLTGVQGTFRETSIQIMHLLRKNKDILLNLLETFIYDPLFDWKHSN 2156          

HSP 2 Score: 95.9005 bits (237), Expect = 1.032e-17
Identity = 68/304 (22.37%), Postives = 145/304 (47.70%), Query Frame = 2

Query: 2738 WWAIHEAARYCISTRLRTNLGGPTQTFAALERMLLDVSHVLQLDGEQNDGSLSIIGSSGAHLLPMRLLLDFVEALKKNVYNAYEGSAVLPSAGRQSSLFFRANKKVCEEWFSRICEPMMNAGLALQCHDATIQYCTLRLQELKNLVVSAFKEKSQAQVTEYLHNMREKYIGDISRIVRHMSLALCRNHESEALIGLQKWVSL-----------------TFSPLLLDEDQSVNHSGIVEPFQWITGLVYQAEGQYEKAVSHFAHLLQTEESLSTMGSDGAQFCIARIIESYTAVSDWKSLESWL 3598
            +W + E +++CI+ RL++  G   QTF   E++LL      QL+ ++ D               +++LL F+E+++K +++   GS ++ S   + + FFR N +VCE+WFSRI   ++ A +        I++ +LR+Q++              Q   ++ +    +  ++   + H++ AL + +E+E+L GL  W  +                   +      + ++N   +  P  W+ G++ +++ ++E+++S    +    ES     S    F + +II+SY  +S++  +E +L
Sbjct:  757 FWTVWECSKFCIANRLKSPYGSAFQTFEIFEKLLL------QLNKDRRD------------FKKIKILLHFMESMEKLIFSTVNGSVLVQSLNNKETQFFRHNVRVCEDWFSRIRVNLLKASILSSSTPDIIRHASLRVQDI--------------QANRFVLDSNTAHF-ELEFCILHLANALQQLNETESLQGLSNWSDINLNNSDNNNNNNNKNNNNNNNSTFFNNGNINKYTLKLP--WMKGVILRSKQKFEESISSLLSVPPMLES----NSISFPFVLEQIIKSYLDISNFTEVEQFL 1021          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy Swiss-Prot
Match: TOR_ORYSJ (Serine/threonine-protein kinase TOR OS=Oryza sativa subsp. japonica GN=TOR PE=2 SV=3)

HSP 1 Score: 261.151 bits (666), Expect = 8.240e-68
Identity = 195/649 (30.05%), Postives = 304/649 (46.84%), Query Frame = 2

Query: 5276 CQSQKQKVGSYTLRATLYVMHVLLNYGL--ELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLVDINAYEEKPSEELQHILGCLRELYPRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKPETPHELWFHHEYKEQLKSAIVSLKTPPASA--AALGDVWRPFDHIAASL-ASYQRKSSISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKSSVPPVPRPSDMFYGKIIPALKEKGIRRVISRRD-WPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKG-QRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEW------------TRGDFHDDAAIGGEERKGMEL 7165
            C S  + V   +L+  L ++ +  N+G   E++  L+   S V +  W  V PQ+ AR+ S+  ++VR+ I+SLL+ + K  P +++YP+LV   +         Q ++  +R+    L+   QLV  EL  V +LW E+W   L               EEA+R+            + N     A++ P+   LER        PET  E  F   Y  +L  A        A+   A L   W  + H+   +       +++ L  V+P+L      ++ +PG     AP             +VTI  F   L ++++K +P+KL I GSDG  Y +LLKG EDLR D R+MQL   +N+ L +S  T+   L I+ Y+V P+S  +GLI WV N  +++++ + +++                               KI    + + +       D  P   K +V    ++      L + LW  S   + +  +   Y+ S+A MSMVG++LGLGDRH  N++LD  SG ++HID+  CF+    R K PE VPFRLT+ +  A+ ++GIEGTFR  CE V+ VLR NKD ++ ++E FV DPLI W              G+ H    +  EE    EL
Sbjct: 1784 CASTTKGVDD-SLQDILRLLTLWFNHGATSEVQMALQKGFSLVNIEMWLVVLPQIIARIHSN-NKIVRELIQSLLVRIGKDHPQALMYPLLVACKSISILRQRAAQEVVDKIRQHSGGLVDQAQLVSKELIRVAILWHEMWHEAL---------------EEASRMY---------FGEHNIEGMLAVLEPLHAMLER-------GPETIKENTFIQAYGHELLEAHECCLKYRATGEDAELTKAWDLYYHVFRRIDKQLPSLTTLDLHSVSPELLECRKLELAVPGTYSADAP-------------LVTIEYFVPQLIVITSKQRPRKLTIHGSDGNDYAFLLKGHEDLRQDERVMQLFGLVNTLLENSRKTSEKDLSIQRYAVIPLSPNSGLIGWVPNCDTLHALIREYRD-----------------------------ARKIFLNQEHRCMLSFAPDYDHLPLIAKVEVFQHALENSEGNDLAKVLWLKSRTSEVWLERRTNYTRSLAVMSMVGYLLGLGDRHPSNLMLDRYSGKILHIDFGDCFEASMNREKFPEKVPFRLTRMLVKAMEVSGIEGTFRTTCENVMQVLRTNKDSVMAMMEAFVHDPLINWRLFNFNEVPQVTNYGNAHSHTVVNSEEAANREL 2357          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy Swiss-Prot
Match: TOR_ARATH (Serine/threonine-protein kinase TOR OS=Arabidopsis thaliana GN=TOR PE=1 SV=1)

HSP 1 Score: 259.996 bits (663), Expect = 1.667e-67
Identity = 194/627 (30.94%), Postives = 306/627 (48.80%), Query Frame = 2

Query: 5276 CQSQKQKVGSYTLRATLYVMHVLLNYGL--ELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLVDINAYEEKPSEELQHILGCLRELYPRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKPETPHELWFHHEYKEQLKSA---IVSLKTPPASAAALGDVWRPFDH----IAASLASYQRKSSISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKSSVPPVPRPSDMFYGKIIPALKEKGIRRVISRRD-WPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKG-QRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEWTRGDFHD 7123
            C +  + V   +L+  L ++ +  N+G   +++  L+   S V + +W  V PQ+ AR+ S+    VR+ I+SLL+ + +  P +++YP+LV   +         Q ++  +R+    L+   QLV +EL  V +LW E+W   L               EEA+R      L   E N     K   ++ P+   L+  +   S    T  E  F   Y+ +LK A     + K      A L   W  + H    I   LAS    +++ L  V+P+L +    ++ +PG  +  AP             +VTI+SFS  L ++++K +P+KL I G+DG+ Y +LLKG EDLR D R+MQL   +N+ L +S  T    L I+ YSV P+S  +GLI WV N  +++ + +  ++                               KII   + K +       D  P   K +V    ++      L + LW  S   + +  +   Y+ S+A MSMVG+ILGLGDRH  N++L   SG ++HID+  CF+    R K PE VPFRLT+ +  A+ ++GIEG FR+ CE V+ VLR NKD ++ ++E FV DPLI W   +F++
Sbjct: 1794 CAANAKGVDD-SLQDILRLLTLWFNHGATADVQTALKTGFSHVNINTWLVVLPQIIARIHSN-NRAVRELIQSLLIRIGENHPQALMYPLLVACKSISNLRRAAAQEVVDKVRQHSGALVDQAQLVSHELIRVAILWHEMWHEAL---------------EEASR------LYFGEHNIEGMLK---VLEPLHDMLDEGVKKDS---TTIQERAFIEAYRHELKEAHECCCNYKIT-GKDAELTQAWDLYYHVFKRIDKQLASL---TTLDLESVSPELLLCRDLELAVPGTYRADAP-------------VVTISSFSRQLVVITSKQRPRKLTIHGNDGEDYAFLLKGHEDLRQDERVMQLFGLVNTLLENSRKTAEKDLSIQRYSVIPLSPNSGLIGWVPNCDTLHHLIREHRD-----------------------------ARKIILNQENKHMLSFAPDYDNLPLIAKVEVFEYALENTEGNDLSRVLWLKSRSSEVWLERRTNYTRSLAVMSMVGYILGLGDRHPSNLMLHRYSGKILHIDFGDCFEASMNREKFPEKVPFRLTRMLVKAMEVSGIEGNFRSTCENVMQVLRTNKDSVMAMMEAFVHDPLINWRLFNFNE 2345          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy Swiss-Prot
Match: TOR_DROME (Target of rapamycin OS=Drosophila melanogaster GN=Tor PE=1 SV=1)

HSP 1 Score: 248.44 bits (633), Expect = 5.646e-64
Identity = 182/607 (29.98%), Postives = 289/607 (47.61%), Query Frame = 2

Query: 5309 TLRATLYVMHVLLNYG--LELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLVDINAYEEKPSEELQHILGCLRELYPRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKPETPHELWFHHEYKEQLKSAIVSLKTPPASAAA--LGDVWRPFDHIAASLA-SYQRKSSISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKSSVPPVPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEV---KRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKGQ-RLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEW 7102
            +L+ TL ++ +  +YG   E+ + L   +  + + +W  V PQL AR+ +H  ++V + I  LLM + K  P ++VYP+ V   +           IL  +R+  P L++   +   EL  V +LW E W   L               EEA+R+              N      ++ P+   LER        P+T  E  F   Y  +L  A    +    SA    L   W  + H+   ++    + +S+ L  V+P+L      ++ +PG      P +           ++ I+    +L ++++K +P+KL I GS+GK Y YLLKG EDLR D R+MQL   +N+ L     T    L I+ Y+V P+S  +GLI WV +  +++++ + +++               K  VP       M      P              D+ H     K +V    + +     L + LW  S   + +  +   Y+ S+A MSMVG+ILGLGDRH  N++LD  SG ++HID+  CF+    R K PE +PFRLT+ +  A+ +TGIEGT+R  CE+V+ VLR+NKD L+ +LE FV+DPL+ W
Sbjct: 1820 SLQDTLRLLTLWFDYGNHAEVYEALLSGMKLIEINTWLQVIPQLIARIDTH-RQLVGQLIHQLLMDIGKNHPQALVYPLTVASKSASLARRNAAFKILDSMRKHSPTLVEQAVMCSEELIRVAILWHEQWHEGL---------------EEASRLYFGDR---------NVKGMFEILEPLHAMLER-------GPQTLKETSFSQAYGRELTEAYEWSQRYKTSAVVMDLDRAWDIYYHVFQKISRQLPQLTSLELPYVSPKLMTCKDLELAVPG---SYNPGQE----------LIRISIIKTNLQVITSKQRPRKLCIRGSNGKDYMYLLKGHEDLRQDERVMQLFSLVNTLLLDDPDTFRRNLAIQRYAVIPLSTNSGLIGWVPHCDTLHTLIRDYRD---------------KKKVPLNQEHRTML--NFAP--------------DYDHLTLMQKVEVFEHALGQTQGDDLAKLLWLKSPSSELWFERRNNYTRSLAVMSMVGYILGLGDRHPSNLMLDRMSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLIKAMEVTGIEGTYRRTCESVMLVLRRNKDSLMAVLEAFVYDPLLNW 2350          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy Swiss-Prot
Match: TOR_DICDI (Target of rapamycin OS=Dictyostelium discoideum GN=tor PE=1 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 1.025e-63
Identity = 179/605 (29.59%), Postives = 297/605 (49.09%), Query Frame = 2

Query: 5309 TLRATLYVMHVLLNYGL--ELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLVDINAYEEKPSEELQHILGCLRELYPRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKPETPHELWFHHEYKEQLKSAIVSLKTPPAS--AAALGDVWRPFDHIAASL-ASYQRKSSISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKSSVPPVPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEVKRKVLLDLMKEVPKQL-LHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKG-QRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEW 7102
            +L+ TL ++ +   +G   E++  L    +T+ + +W  V PQ+ AR+ + P   VR+ +  L+  + K  P ++VYP+ V   ++        + ++  +R+    L+     V  EL    +LW E+W   L               EEA+R               N     A +AP+   LE+        PET  E  F   +   L+ A+   K    +     L   W  +  +   +     + SS+ L  V+P+L   ++ ++ +PG  K             A   ++ I SFS+ L+++ +K +P+KL I+GSDG  YT+LLKG EDLR D R+MQL   +N+ L ++  T  + L IR +SV P+S  +GLI WV +  +++++ K +++                               KI+ +++ + + ++ S  D    +++  +     E    L LH+ LW  S   + +  +   Y+ S+A MSMVG+ILGLGDRH  N++LD  +G ++HID+  CF+    R K PE +PFRLT+ +  A+ ++GIEG FR  CEAV++VLR NK+ L+ +LE FV DPLI W
Sbjct: 1707 SLQDTLRLLTLWFKHGAQKEVEAALMQGFNTISIDTWLHVIPQIIARIHA-PVLPVRRLLHELIDTIGKEHPQALVYPLTVATKSHSPARLAAAKSLMDKMRKHSATLVDQALPVSQELVRTAILWLEMWYEGL---------------EEASR---------QYFGDHNPEAMLATLAPLHQILEK-------GPETTSETSFLQAFGRDLQEALEWSKKYEKTRKEGDLNQAWDLYYQVFRRIYKQLPQMSSLELQYVSPKLLNSNNMELAVPGTYK-------------ASENVIRIQSFSQALSVIPSKQRPRKLTIIGSDGLEYTFLLKGHEDLRQDERVMQLFSLVNNLLSANHETAKSHLSIRRFSVIPLSPNSGLIGWVPHSDTLHTLIKDFRDS-----------------------------NKILLSIEHRLMLQMCSDYDNLTLLQKVEVFQYALENSNGLDLHKVLWLKSRNSEVWLDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRHTGHILHIDFGDCFEVAMHRDKYPEKIPFRLTRMLINAMEVSGIEGNFRLTCEAVMNVLRNNKESLMAVLEAFVHDPLINW 2237          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy TrEMBL
Match: A0A078IXJ7_BRANA (BnaA07g36310D protein (Fragment) OS=Brassica napus GN=BnaA07g36310D PE=4 SV=1)

HSP 1 Score: 2683.67 bits (6955), Expect = 0.000e+0
Identity = 1423/2323 (61.26%), Postives = 1764/2323 (75.94%), Query Frame = 2

Query:  284 DSARLAAINSLHRAIIYPPNSILVAHSASFLAQGFSQLLSDKSYAVRQSXXXXXXXXXXVVCSVPIGSSGRQNHVMLSSLVDRFIGWALPLLSNISAGDGTTELALEGLREFLSVGDVGAIERYALPILKACQELLEDERTXXXXXXXXXXXXXXXXXXXXXXFQPHFLDIIDLLLGWALVPDLAESDRRVIMDSFLQFQKHWVGNLQFSLGLLSKFLGDMDVLLQDGTHGTPQQFRRLLALLSCFSTVLQSTASGLLEMNLLEQISEPLSKMLPRLLGCLSVVGKKFGWSRWIEDSWKCLTLLAEILQERFSTFYPLAIDILFQSLELGNTSRPIGAGKITSFQVHGVXXXXXXXXXXXXXXXXPSSVKKILHFDRAISQLRLHPNHLVTGSSAATYVFLLQQGNDEIVQQAXXXXXXXXXXXXXXXXXXIVHGEEDNSAGDLRCY---SKFELFALIKFDLKVLLTSVSLCGCNNLIVQPKIAALYLQRSESLISFIIEKLSPFESPIQFCVELQVHVIKTLDRLSMVKFFSKCSIRNQSGNIPVGDAATEKVLDGNSLRDVHPAVIIEYLREFGTLLVRALHVSSPVAVKIVALEWVQRFCENLISICESPSMRYNFYEEFGYVSLFGDLIFSILEAAFDREPKVRLHVTLSLELLLQARLMHPLYFNSVSDVVLEKLGDPDADIRNAYVRLLSHVLLTTMYVYGIHDIGACSNSRPRALMLGNSSNLYWKQVFALKQLPQQLHSQQLVSILSYISQRWKVPLSSWIQRLIHTCRSSKDSSLGQLEETGIVGANDLWLDIKMEN-ILEKFCSVNNLAGAWWAIHEAARYCISTRLRTNLGGPTQTFAALERMLLDVSHVLQLDGEQNDGSLSIIGSSGAHLLPMRLLLDFVEALKKNVYNAYEGSAVLPSAGRQSSLFFRANKKVCEEWFSRICEPMMNAGLALQCHDATIQYCTLRLQELKNLVVSAFKEK--SQAQVTEYLHNMREKYIGDISRIVRHMSLALCRNHESEALIGLQKWVSLTFSPLLLDEDQSVNHSGIVEPFQWITGLVYQAEGQYEKAVSHFAHLLQTEESLSTMGSDGAQFCIARIIESYTAVSDWKSLESWLLELQTLRAKYAGKSYSGALTTAGNEMNAIHALAQFDEGDLQAAWAYLDLTPKSSSELTLDPKLALQRSEQMLLQALLFKIEGNLDKVPHELQKAKSMLEEMLSVLPLDGLAEATACVTQLHCIFAVGEGYDLSQGNCWKHMGSQGKSKLNQSGLGSYLLPLQPLIKGIHQDCNPWLKVLRVYRTISPTSPVTLKLSMNLMSLARKQGNLTLANCLINYLRDHVSSCSHERYCNLLNLNLQYEGILILHAENKIEDAFANIWSFLRPYLCSSSFIVNDVDDGLMKAKACLKLSNWLRQNYSSLNLRNIVLRMLSDLNVATVSSSGTGGYSFSDENL---SSKWSLDVIVEEIVGTATKLSTQLCPKMAKSWISYASWCFNQAKSSVANQQEKCLQSCSFSPILVSELTPKRFKMTEDEIQIVESVILPLFQKRDSAEHVDDGAEQWGFCSGSAENLRTNNMLMTLVQQLVDIMEAAAGAPGTENSGGERXXXXXXXXXXXXXQHANFGIEETHLTYVIDKLIDIWWSLRRRRVSLFGHAAHAFIKYLLYSSTKLSDGRLSGDFCQSQKQKVGSYTLRATLYVMHVLLNYGLELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLVDINAYEEKPSEELQHILGCLRELYPRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKPETPHELWFHHEYKEQLKSAIVSLKTPPASAAALGDVWRPFDHIAASLASYQRKSSISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKS-SVPPVPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKGQRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEWTRGDFHDDAAIGGEERKGMELAVSLSLFASRVQEIRVPLQ 7222
            DSARLAA+ SLHRAI YPPNS+L+A+SAS+L  G  QLLSDKSY VRQ++  AY ALCA+VCS+PI   GRQ+ +ML   VD FI WALPL+ ++S  DG+T LALE +REFLSVGDV  I+RYALPIL AC+ LLEDERTS+ LL++ L VL LI  KF + F PHFLDI+D+LLGWAL+ D  +SDR++++DSFLQFQKHWVGNL FS+GLLSKFLGDMDVLL DG+ GTP+QF RLLALLSCF  +L+STASGLLE NLL ++ EPLS+M+PRLLG LS +G++FGW++WIED WKCLTLLAEIL+E+FST+Y  ++++LF+SLE  +           +  +HG LKTNLQLLSLQK GLL S V K+L F+  +S+LRLH N LVT  S+ TY+FLLQ  ++EI+QQAM LL  ELELLK +L   +     +N   D+  Y   SK EL A+I FD+KVLL+ VSL G  +L   PKIAALYL+RSE LISF+ EKL+PFES +  C ELQV +++ L+ L+ V+  S CS+  Q G+  + +    K      ++     +++E+LR++   +V+AL  SSP+ VK+ +LEW+Q+FCENL++I ++ +M   F E F Y     D+I ++L+AAFD EPKVR      LELLL  +L+HP+ F S+++++LEKLGDPD  I+NA+++LLSH+L  T YV G+H        RP  LMLGN   L+WKQVFALKQ      SQQLVS+L+Y+SQRWKVP SSWIQ LI+  + +KD+  G  +E  I      WL IK E   +E+ C  NNLAGAWWA+HEAAR+C+STRLRTN GGPTQTFA LERMLLD++ VLQ+D EQN     ++GSSGAHLLPMRLLLDFVEALKKNVYNAYEG+AVL SA +QS LFFRAN+KVCEEWFSRI EPMMNAG++LQ H+AT++YCTLRL+ELK+  +   KEK   QAQ+T            DIS I+R MSLAL +NH++ AL+G+ KWVS+  +PL+ +E  +   S  +  F WITGL+YQ++G+YEKA ++FAHLL+ E+ LS+MGSD   F I RIIESY A+SDWKSL+SWL ELQ LRA++AGKS+SG+LT AGNEMNAI ALA+FDEGD+Q AWA LDLTPK+S+E +L+PKLALQRSEQMLLQA+LF+ EGN+ KV H LQKA+SML+E    L  DGL+E     TQLHC++A  E + + +        S+ K K N   L S +  +Q +I  IH+DC+PWLKVLR Y TISPT+ VTLKL M+L   ARKQGNL LAN L  YL DHVSSC   +  + L  NLQY+G L+++ ENKI+DA  ++WSF++P + +   +  D     +KAKACLKL+ WL+ + SSL+L  +V ++ +D N   + SS       S E L   SS+ S+  I EE++GT TK+STQLC  M KSWISYASWCF QA  S +   E  + +   S IL  EL P  F++TEDE Q VES+++   Q  +S +  + G +     + +A++  +   +  L +Q+++ +E AA APG E    + LS  LASQL  +L  A    E+T +  ++ +LI++WWSLR+RRVSLFGH+A  F +YL YSS  L     +G       +K GS+TLR+TLY++H+LLNYG+ELKDTL  ALS VPL  WQ+VTPQLFARLSSHPEEVVRK+IE LL+MLAKL P SIVYP LVD+NA +EKPSEEL H+  CL ELYPRLIQDVQL++NELGNVTVLWEELWLSTLQDLHMDV+RRIN+LKEEAAR++EN TLSQSEKN INAA+YSAMMAPIVVALERRLASTSRKP TPHE+WF+ EY E+L SAI++ KT P S AALG+VWRPFD IA SLAS+Q+KSSISL EVAP L++LSS ++PMPGLEKQ    ESD    ++L GIVT++S S+H+TIL TKT+PKKL+++GSDGK Y YLLKGREDLRLDARIMQLLQAIN+F  SS AT+   +GIRYYSVTPISGRAGLIQWVDNVISIYSIF+SWQ R +L+Q+S    GSAKS  +PPVPRPSDMFYGK+IPALK+KGIRRVISRRDWPH+VKR+V LDLMKEVPKQLLH+ELWCASEGFKAF +K KRYSGSVAAMS+VGH+LGLGDRHLDNIL+DF SGDVVHIDYNVCFDKGQRL+VPEIVPFRLTQT+EAALGLTG+EGTFRANCEAV+ VLR+NKD LLML+EVFVWDPL+EWTRG+ HDDAAIGGEERK ME+AVSLSLF+SRVQEIRV LQ
Sbjct:   83 DSARLAALKSLHRAITYPPNSLLIANSASYLCSGLWQLLSDKSYDVRQASVSAYGALCAIVCSIPIEFIGRQSLLML---VDSFIDWALPLIRDVSVQDGSTVLALESVREFLSVGDVHVIDRYALPILDACKSLLEDERTSIPLLNQALGVLFLISSKFFILFHPHFLDIVDMLLGWALLQDQPDSDRQIVLDSFLQFQKHWVGNLDFSVGLLSKFLGDMDVLLCDGSEGTPEQFHRLLALLSCFLAILRSTASGLLENNLLYKMDEPLSQMIPRLLGFLSTMGQRFGWAKWIEDLWKCLTLLAEILREKFSTYYFPSLEMLFKSLEQND-----------NVNIHGTLKTNLQLLSLQKQGLLSSCVPKLLCFESPLSRLRLHQNRLVTAGSSDTYIFLLQHESEEIIQQAMELLMNELELLKTVL---VDSSGSENKTFDVTAYEAFSKNELLAMINFDMKVLLSCVSLGGRKSLSDVPKIAALYLKRSEKLISFVFEKLNPFESYVDACSELQVSIMRMLECLAAVELLSNCSVLIQPGSKLLKETNHGKT----PVKHTFFTMVVEHLRKYSLTMVKALRFSSPLDVKLASLEWIQKFCENLMAISKNLNMDAYFCETFPYAGAVRDVIMAVLDAAFDTEPKVRSLSAKVLELLLVVKLIHPISFYSLAEILLEKLGDPDTSIKNAFIKLLSHLLPATQYVCGMHSEVEHMALRPEVLMLGNGY-LHWKQVFALKQPDHHFQSQQLVSVLNYVSQRWKVPFSSWIQGLINAFQGTKDTDDGHPDENLIKNG---WLAIKAEKGSIERICLANNLAGAWWAVHEAARFCVSTRLRTNFGGPTQTFAVLERMLLDITTVLQVDSEQN-----VVGSSGAHLLPMRLLLDFVEALKKNVYNAYEGTAVLSSAPQQSVLFFRANRKVCEEWFSRISEPMMNAGISLQSHNATVEYCTLRLEELKSRAILVKKEKFKVQAQIT-----------SDISGILRKMSLALSQNHDTHALLGVNKWVSMNLAPLVAEESDNQKRSDELTLFPWITGLIYQSDGRYEKAAAYFAHLLEEEDCLSSMGSDDIHFVIERIIESYAALSDWKSLDSWLSELQALRARHAGKSFSGSLTAAGNEMNAIRALARFDEGDIQGAWACLDLTPKTSAEPSLNPKLALQRSEQMLLQAILFQAEGNIQKVSHTLQKARSMLDETSLALSFDGLSETAPYATQLHCLYAFEESHQMRE--------SEPKQKHNNLMLSSCVWSMQSMINRIHRDCSPWLKVLRTYSTISPTAWVTLKLCMDLFGFARKQGNLLLANHLKVYLNDHVSSCDEVKVRDFLISNLQYQGALLMYEENKIQDAVVDLWSFVQPEVTALHPVCLDNGVAFLKAKACLKLATWLKGDASSLDLEKVVFKISADFNRTEIPSS------VSSEPLVYESSQPSIKAISEEMIGTVTKVSTQLCSAMGKSWISYASWCFRQATESFSKSNESTIHTSFSSRILAQELQPGTFRLTEDETQSVESIVMQFLQNDESKDLTNIG-QDGNHHTTTADH--SEARMKALQKQVIETIENAAAAPGGEGCDWDSLSVHLASQLTDTLLCAKENGEDTDIGPIVKRLIEVWWSLRKRRVSLFGHSALGFTQYLRYSSKNLRTSEFTGVDYDPLNKKAGSHTLRSTLYILHILLNYGVELKDTLRHALSMVPLEPWQEVTPQLFARLSSHPEEVVRKEIEGLLIMLAKLCPWSIVYPTLVDVNACDEKPSEELLHVKACLAELYPRLIQDVQLMLNELGNVTVLWEELWLSTLQDLHMDVIRRINLLKEEAARVSENVTLSQSEKNDINAARYSAMMAPIVVALERRLASTSRKPVTPHEVWFYEEYIERLNSAILTFKTLP-SPAALGEVWRPFDSIATSLASHQKKSSISLKEVAPSLSLLSSCNIPMPGLEKQPTLSESD----TSLQGIVTVSSLSDHVTILPTKTRPKKLIMIGSDGKKYIYLLKGREDLRLDARIMQLLQAINTFFCSSRATDGGTIGIRYYSVTPISGRAGLIQWVDNVISIYSIFRSWQTRAKLSQMSPSAPGSAKSPDLPPVPRPSDMFYGKMIPALKDKGIRRVISRRDWPHDVKRQVQLDLMKEVPKQLLHRELWCASEGFKAFATKFKRYSGSVAAMSIVGHMLGLGDRHLDNILMDFCSGDVVHIDYNVCFDKGQRLEVPEIVPFRLTQTMEAALGLTGVEGTFRANCEAVLGVLRQNKDTLLMLMEVFVWDPLVEWTRGNSHDDAAIGGEERKDMEVAVSLSLFSSRVQEIRVRLQ 2342          

HSP 2 Score: 365.925 bits (938), Expect = 1.356e-97
Identity = 216/412 (52.43%), Postives = 286/412 (69.42%), Query Frame = 3

Query: 7509 LQEHHDLLLATFPAVESALERFRDVLNQYELVSALFYRADQERSNLILHETSAKSIVAEATCNSEKICASFEIQAREFKQAKNVVAEKAQQATTWIEQHGRILDALRGNLIPEISAGMNLSGMADALSLTSAVPEAGVPLTIVPEPTQAQCYDIDREVSQLIAELDRGLSSAVMALQAYSLALQRVLPLNYLTTSTVHGWAQVLQLSANAVSSDILSLARRQASELIAKVHGDNLEFMKSSHDDLCFXXXXXXXXXXXXXXXXXXXXNSIGSKTESKAKDRLMFAFMKYMQSAGLVRKEDANSSLQYGESKYDGTKAS--RIREDLEEKKDKVLSVLSVAMRSLYDDVKHRILEIYSHTNRAQIENSRPQSDLGTVFSGFEEQVEKCILVAGFVNELWQQIGGDMPGVDRDL 8738
            LQEHHDLLLA  PAVE +LERF  VLNQYE+ S++F +ADQERS L L ETSAK+ VAEATCN E I ASFEIQA+EF +AK +V+EKAQ+   W+EQ G+IL ALR ++IPEI+A   L+ +  +LSLTSAV  AGV LT+VPEPTQAQC +ID+E+S L+ +L  GLSSA+ ALQ YSLALQR+LPLNY TTS V+ WAQVLQL+A A+S+DILSLA+RQA E  AKVHGD+ + +++ +DDL  KVEKYA +++K+EEE AEL  S  +  ESKAK+RL +  + YMQS G V   +A  S +Y  +K    + S  R  +D  E+  K L +L  ++ SLYD  K ++  I + +      N    S+  ++ +  E Q+E C++V  F+NE+    G ++  V   L
Sbjct: 2341 LQEHHDLLLAALPAVELSLERFSKVLNQYEIASSVFLQADQERSKLSLRETSAKTTVAEATCNLENIRASFEIQAQEFSRAKALVSEKAQETAVWMEQRGKILGALRRDMIPEITAPTVLTDILGSLSLTSAVLVAGVRLTVVPEPTQAQCNNIDKEISLLVNDLSDGLSSALAALQTYSLALQRILPLNYHTTSQVYDWAQVLQLAARALSTDILSLAKRQAGEQYAKVHGDDSQSVRNCYDDLWRKVEKYADDVKKLEEEYAELEASSATGPESKAKNRLFYGLINYMQSPGFVESTNAGLSDKYEAAKRVNLQDSGKRTLKDSGERTLKALDLLHTSISSLYDRSKEKVHYILNASTERNDLNESLVSESRSLSTNLEAQIEMCMIVVDFLNEVKHYAGQEISSVGESL 2752          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy TrEMBL
Match: V4LK65_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10022517mg PE=4 SV=1)

HSP 1 Score: 2674.43 bits (6931), Expect = 0.000e+0
Identity = 1417/2321 (61.05%), Postives = 1758/2321 (75.74%), Query Frame = 2

Query:  284 DSARLAAINSLHRAIIYPPNSILVAHSASFLAQGFSQLLSDKSYAVRQSXXXXXXXXXXVVCSVPIGSSGRQNHVMLSSLVDRFIGWALPLLSNISAGDGTTELALEGLREFLSVGDVGAIERYALPILKACQELLEDERTXXXXXXXXXXXXXXXXXXXXXXFQPHFLDIIDLLLGWALVPDLAESDRRVIMDSFLQFQKHWVGNLQFSLGLLSKFLGDMDVLLQDGTHGTPQQFRRLLALLSCFSTVLQSTASGLLEMNLLEQISEPLSKMLPRLLGCLSVVGKKFGWSRWIEDSWKCLTLLAEILQERFSTFYPLAIDILFQSLELGNTSRPIGAGKITSFQVHGVXXXXXXXXXXXXXXXXPSSVKKILHFDRAISQLRLHPNHLVTGSSAATYVFLLQQGNDEIVQQAXXXXXXXXXXXXXXXXXXIVHGEEDNSAGDLRCYSKF---ELFALIKFDLKVLLTSVSLCGCNNLIVQPKIAALYLQRSESLISFIIEKLSPFESPIQFCVELQVHVIKTLDRLSMVKFFSKCSIRNQSGNIPVGDAATEKVLDGNSLRDVHPAVIIEYLREFGTLLVRALHVSSPVAVKIVALEWVQRFCENLISICESPSMRYNFYEEFGYVSLFGDLIFSILEAAFDREPKVRLHVTLSLELLLQARLMHPLYFNSVSDVVLEKLGDPDADIRNAYVRLLSHVLLTTMYVYGIHDIGACSNSRPRALMLGNSSNLYWKQVFALKQLPQQLHSQQLVSILSYISQRWKVPLSSWIQRLIHTCRSSKDSSLGQLEETGIVGANDLWLDIKME-NILEKFCSVNNLAGAWWAIHEAARYCISTRLRTNLGGPTQTFAALERMLLDVSHVLQLDGEQNDGSLSIIGSSGAHLLPMRLLLDFVEALKKNVYNAYEGSAVLPSAGRQSSLFFRANKKVCEEWFSRICEPMMNAGLALQCHDATIQYCTLRLQELKNLVVSAFKEKSQAQVTEYLHNMREKYIGDISRIVRHMSLALCRNHESEALIGLQKWVSLTFSPLLLDEDQSVNHSGIVEPFQWITGLVYQAEGQYEKAVSHFAHLLQTEESLSTMGSDGAQFCIARIIESYTAVSDWKSLESWLLELQTLRAKYAGKSYSGALTTAGNEMNAIHALAQFDEGDLQAAWAYLDLTPKSSSELTLDPKLALQRSEQMLLQALLFKIEGNLDKVPHELQKAKSMLEEMLSVLPLDGLAEATACVTQLHCIFAVGEGYDLSQGNCWKHMGSQGKSKLNQSGLGSYLLPLQPLIKGIHQDCNPWLKVLRVYRTISPTSPVTLKLSMNLMSLARKQGNLTLANCLINYLRDHVSSCSHERYCNLLNLNLQYEGILILHAENKIEDAFANIWSFLRPYLCSSSFIVNDVDDGLMKAKACLKLSNWLRQNYSSLNLRNIVLRMLSDLNVATVSSSGTGGYSFSDENL---SSKWSLDVIVEEIVGTATKLSTQLCPKMAKSWISYASWCFNQAKSSVANQQEKCLQSCSFSPILVSELTPKRFKMTEDEIQIVESVILPLFQKRDSAEHVDDGAEQWGFCSGSAENLRTNNMLMTLVQQLVDIMEAAAGAPGTENSGGERXXXXXXXXXXXXXQHANFGIEETHLTYVIDKLIDIWWSLRRRRVSLFGHAAHAFIKYLLYSSTKLSDGRLSGDFCQSQKQKVGSYTLRATLYVMHVLLNYGLELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLVDINAYEEKPSEELQHILGCLRELYPRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKPETPHELWFHHEYKEQLKSAIVSLKTPPASAAALGDVWRPFDHIAASLASYQRKSSISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKS-SVPPVPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKGQRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEWTRGDFHDDAAIGGEERKGMELAVSLSLFASRVQEIRVPLQ 7222
            DS RLAA+ SLHRAI Y PNS+L+AHSAS+L+ G  QLLSDKSY +RQ+   AY ALCAVVCS+P+ SSG QN +ML   VD FI WALPL+ ++S  DG+T LA+EG+REFLSVGDV  I RYALPIL AC+ LLEDERTS+ LL++ L VL +I  KF   FQPHFLDI+D+LLGWAL+ D A+SDR+++MDSFLQFQKHWV NL+FS+GLLS+FLGDMDVLL DG+ GTP+QF RLLALLSCF  +L+STASGLLE NLL+++ EPLS+M+PRLLG LS +G++FGW++WIED WKCLTLLAEIL+++FST+YP ++++LF+SLE  +T      GK+T+ Q+HG LKTNLQLLSLQK G+L S V K+L F+  IS+LRLH N LVT  S+ TY+FLLQ  ++E+VQQ M LL +ELELLK +L   +   +  N   D+  Y  F   EL A+I FD+KVLL+SVSL G  +L   PK+AALYL+RSE L+SF+ EKL+PFES I  C ELQV +++ L+ L+ V+  S CS+  Q    P   A  E        +     ++IE+L+++   +VRAL +SSPV VK+ +LEW+Q+FCENLI+I ++ ++   FY+ F +     D+I ++L+AAFD EPKVR    + LELLL+ +L++P++F S+++++LEKLGDPD  I+NA+++LLSH+L  T Y  G+H        RP+ LMLGN   L+WKQVFALKQ      SQQLVS+L+Y+SQRWKVP +SWIQ LI+  + SKD+   Q +E  I      WL IK E + +E+ C  NNLAGAWWA+HEAAR+C+STRLRTN GGPTQTFA LERMLLD++ VLQ+D EQ +     +GSSGAHLLPMRLLLDFVEALKKNVYNAYEG+AVL S+  QS LFFRAN+KVCEEWFSRI EPMMNAG++LQ HDAT++YCTLRL+EL++      KEKS+ Q  + +++       DISRI+RHM+LALC+NH++ AL+G+ KWVS+ F+PL+ +E      SG +  F WITGL+YQ+EG+YEKA +HFAHLL+ E+SLSTMGSD  QF I RIIESYTA+SDWKSL+SWL ELQ LRA++AGKS+SG+LT AGNE+NAI ALA+FDEG++QAAWA LDLTPK+S EL+L+PK+ALQRSEQMLLQA+L + EGN  +VP  LQKA+SML+E    L  DGL+E     TQLHC+FA  E + L          S+ K K +   L S +  +Q +I  IH+DC+PWLKVLR+YRTI PTS VTLKL M+L+  ARKQGN  LAN L  YL DHVSSC   +  + L  NLQY+G L+++AENK++DA  ++WSF++P + +   +  D     +KAKACLKL+ WL+ +  SL+L N+VL+M ++ N   + S      S S E L   SSK S+  I EE++GT TK+STQLC  M KSWISYASWCF QA  S     +  L S SFS IL  E+ P RF +TEDE Q VES++  L QK D  +  D G +   + + +A++  ++     L QQL++ +E AA APG EN   + LS  LASQL   L  A   +E+T +  ++ KLI++WWSLR+RRVSLFGHAA  F +YL YSS  L     +G       +K GS+TLR+TLY++H+LLNYG+ELKDTL  ALS VPL  WQ+VTPQLFARLSSHPEEVVRK+I+ LL+MLAKL P SIVYP LVD+NA +EKPSEEL H+   L     R I D     N     +  + E       D   DV+RRIN+LKEEAAR++EN TLSQSEK++INAA+YSAMMAPIVVALERRLASTSRKPETPHE WFH EY E++KSAI++ KTPP    ALG+VWRP D IAASLAS+Q+KSS+SL EVAP L++LSS ++PMPGLEKQ    ESD    ++LHGIVTI+S S+H+ IL TKT+PKKLV+ GSDGK Y YLLKGREDLRLDARIMQLLQAIN+F  SS +T+   +GIRYYSVTPISGRAGLIQWVDNVISIYSIF+SWQ RV++AQ+     GSAK+  +PPVPRPSDMFYGKIIPALKEKG+RRVISRRDWPH+VKRKVLLDLMKEVPKQLLH+ELWCASEGFKAF  KLKRYSGSVAAMS+VGH+LGLGDRHLDNIL+DF SGDVVHIDYNVCFDKGQRL+VPEIVPFRLTQT+EAALGLTG+EGTFRANCEAV+ VLRKNKD+LLML+EVFVWDPL+EWTRG+FHDDAAIGGEERK ME+AVSLSLF+SRVQEIRV LQ
Sbjct:   82 DSGRLAALKSLHRAITYLPNSLLIAHSASYLSSGLWQLLSDKSYDMRQAGVSAYGALCAVVCSIPVESSGIQNLMML---VDSFIDWALPLIRDVSVQDGSTVLAVEGVREFLSVGDVNVIGRYALPILDACKSLLEDERTSIPLLNQALGVLLVISSKFYSLFQPHFLDIVDVLLGWALLQDQADSDRQIVMDSFLQFQKHWVDNLEFSVGLLSRFLGDMDVLLSDGSQGTPEQFHRLLALLSCFLAILRSTASGLLENNLLDKMDEPLSEMIPRLLGFLSTMGQRFGWAKWIEDLWKCLTLLAEILRDKFSTYYPPSLEMLFKSLEQIDTVNSAVHGKLTTSQIHGTLKTNLQLLSLQKRGILSSYVPKLLSFESPISRLRLHSNRLVTACSSDTYIFLLQHESEELVQQVMELLLKELELLKTVL---VDPSDLQNKTFDVTVYESFSKNELLAMINFDMKVLLSSVSLGGRKSLSDVPKLAALYLKRSEKLVSFVFEKLNPFESYIDTCSELQVSIVRMLECLAAVELLSNCSVLIQ----PENKALKELNHGKTPFKCTFFTMVIEHLKKYSVTMVRALRLSSPVGVKLGSLEWIQKFCENLIAISKNFNIDAYFYKSFPFAGAVRDIIMAVLDAAFDSEPKVRSRSAMVLELLLEVKLINPIHFYSLAEILLEKLGDPDTSIKNAFIKLLSHLLPATQYACGMHSEVEHRALRPQVLMLGNGY-LHWKQVFALKQPDHHFQSQQLVSVLNYVSQRWKVPFTSWIQGLINAFQGSKDTGAKQPDENLIKNG---WLAIKAEKSSIERICLANNLAGAWWAVHEAARFCVSTRLRTNFGGPTQTFAVLERMLLDITTVLQVDSEQTN-----VGSSGAHLLPMRLLLDFVEALKKNVYNAYEGTAVLSSSLPQSVLFFRANRKVCEEWFSRISEPMMNAGISLQSHDATVEYCTLRLEELRSRATLVKKEKSKMQDFDNVNSAGAHIYSDISRILRHMALALCQNHDAYALLGVHKWVSINFAPLVAEESNLQKLSGDLALFPWITGLIYQSEGRYEKAAAHFAHLLEEEDSLSTMGSDDIQFVIERIIESYTALSDWKSLDSWLSELQALRARHAGKSFSGSLTAAGNEINAIRALARFDEGNIQAAWACLDLTPKTSVELSLNPKVALQRSEQMLLQAMLLQAEGNAQRVPPTLQKARSMLDETSLALSFDGLSETAPYATQLHCLFAFEESHQLRD--------SEPKQKHSNLMLSSCVWSMQSMINRIHRDCSPWLKVLRIYRTILPTSWVTLKLCMDLLGFARKQGNFLLANHLKEYLNDHVSSCDEVKLQDFLISNLQYQGALMMYAENKVQDAVVDLWSFVQPEVTALEPVCLDAGVAFLKAKACLKLATWLKGDVISLDLENVVLKMSTNFNRTEIPS------SVSREPLLYESSKPSIKAISEEMIGTVTKVSTQLCSAMGKSWISYASWCFRQATESSCKPNDSTLHSFSFSSILAQEIQPGRFHLTEDETQSVESIVKELLQKDDIKDLTDTG-QDGNYHTTTADH--SDERKKALQQQLIETIENAAAAPGAENCDWDSLSVHLASQLTDLLLCAKQNVEDTDIAPIVKKLIEVWWSLRKRRVSLFGHAALGFTQYLRYSSKNLHTSEFTGVDYDPLNKKSGSHTLRSTLYILHILLNYGVELKDTLGHALSMVPLEPWQEVTPQLFARLSSHPEEVVRKEIKGLLIMLAKLCPWSIVYPTLVDVNACDEKPSEELLHVKAYLNR---RNILDHDCSAN-----SATYNE------SDSVSDVIRRINLLKEEAARVSENVTLSQSEKDEINAARYSAMMAPIVVALERRLASTSRKPETPHEDWFHEEYIERIKSAILTFKTPPL-PDALGEVWRPLDSIAASLASHQKKSSVSLKEVAPSLSLLSSCNIPMPGLEKQPTLSESD----TSLHGIVTISSLSDHVNILPTKTRPKKLVMFGSDGKKYIYLLKGREDLRLDARIMQLLQAINTFFCSSRSTDGGSIGIRYYSVTPISGRAGLIQWVDNVISIYSIFRSWQTRVKVAQMPPSAPGSAKNPDLPPVPRPSDMFYGKIIPALKEKGVRRVISRRDWPHDVKRKVLLDLMKEVPKQLLHKELWCASEGFKAFTIKLKRYSGSVAAMSIVGHMLGLGDRHLDNILMDFCSGDVVHIDYNVCFDKGQRLEVPEIVPFRLTQTMEAALGLTGVEGTFRANCEAVLGVLRKNKDILLMLMEVFVWDPLVEWTRGNFHDDAAIGGEERKDMEVAVSLSLFSSRVQEIRVRLQ 2347          

HSP 2 Score: 1166.37 bits (3016), Expect = 0.000e+0
Identity = 652/1168 (55.82%), Postives = 841/1168 (72.00%), Query Frame = 3

Query: 7509 LQEHHDLLLATFPAVESALE---------RFRDVLNQYELVSALFYRADQERSNLILHETSAKSIVAEATCNSEKICASFEIQAREFKQAKNVVAEKAQQATTWIEQHGRILDALRGNLIPEISAGMNLSGMADALSLTSAVPEAGVPLTIVPEPTQAQCYDIDREVSQLIAELDRGLSSAVMALQAYSLALQRVLPLNYLTTSTVHGWAQVLQLSANAVSSDILSLARRQASELIAKVHGDNLEFMKSSHDDLCFXXXXXXXXXXXXXXXXXXXXNSIGSKTESKAKDRLMFAFMKYMQSAGLVRKEDANSSLQYGESKYDGTKASRIREDLEEKKDKVLSVLSVAMRSLYDDVKHRILEIYSHTNRAQIENSRPQSDLGTVFSGFEEQVEKCILVAGFVNELWQQIGGDMPGVDRDLYYSKYYSEGNWASIFKTILNCCKSLIGEMTEVVLPDIMRSAVSFNTEVMDAFGLISQIRGSIDTALEQLVEVELERASLAELEQNYFVKVGLITEQQLALEEAAMKGRDHLSWXXXXXXXXXXXXCRAQLEQLHQTWNHRDMRT-SSLIKREAEIKSSLVSCEQHFQSLITGDDFRESHRSKSQVLLAILVKPFSELESVDKAXXXXXXXFAPHSDEIPNLVEFLSSGHSVSECVWNFGSLLNNHSFFIWKIGVLDSILDLCIHDMASSVDQNLGFEQLFDVVKRKLEIQLQEYIGRYLKIRIAPALLSWLDKENEHLKLLTEGAKEPSNDHVRKDVEAVKKVQLMLEEYCNTHETARAARSAASVMKRQVNELKEALRKTILEIVQMEWMHDVGLTPSHTSRVLFQKFFSNDDELYPVVLNFSRPKLLETMQSVLSKLARAIEGLKSCEHTSIVAEGQLERAMGWACGGPNSSVAGNSSTKASGIPPEFHDHLMRRRHLLQEAREKASSVVKICMSILEFEASRDGIFQIPREGYALSTG--SDSRTWQQAYFNALTKLEVTYHSFTRIEQEWKLAQSNMEVASSGLYSATNELCIASLKAKSASGDLQSTVLAMRDCAYEASVALSAFAHVSRGHTALTSESGSMLEEVLAITEDLHDVHNLGKEAASVHRSLMEDLSKANAILLPLESVLAKDVSAMTEAMARERETKMEVSPIHGQAIYQSYGLRVREACQTFKPLVPSLTFSVK 10976
            LQEHHDLLLAT PAVE +LE         RF  VLNQYE+ S++F +ADQERS LIL ETSAK  VAEA CNSE I ASFEIQA+EF Q K +V+EKAQ++  W+EQ GRIL ALR NLIPEI+A   L+    +LSLTSAV  AGV LT+VPEPTQ QC +ID E+S L+ +L  GLSSA++ALQ YSLALQR+LPLNY TTS V+ W QVLQL+A+A+S+DILSLA+RQA E  AK+ G +   + + +DDLC KVEKYA +++KVE E AEL  S+G   E KAKDRL +  + YMQS   V   +A  S     +K++ T+  R++    +K  K L++L  ++ S YD +K ++  I + +   +  +    S+  ++ +  E QVE C+++  F+NE+   +G ++  V+  L  S +  + NWA IF   L   K+LI  MTE V+PD++++ +  N++ MDAFGLISQIRGSIDTALE L+E+++ER SL EL+Q+YF KV  ITE QLALE+AA+K R+HLSWEEAEE +SQ EACR Q++QL+Q+W  R+ +  SSLIK+EA++K++L+S E+ FQ L   ++  + +  KS  +L  LVKPFSEL+ +DK LSSLSSS    SD IP   + L  G S+SE +  F S+L +HSFFIWK+ ++ S LD CIHD   S DQ LGFEQL  ++K+K E QLQE +  YL   +APA LS LDKENE LK  +E     S D V+ +   +K+V  MLEEYCN HETA AA+SAAS MK Q+ E+++ALR+T L+IVQMEWM+D  LTPS   R   Q+  ++DD L+P+ L+ +R KLLE+++S + ++ R+IE L++CE  SI AEGQLERAMGWACGG       +SS K SGIP EFHDHL RRR LL +AREKAS++ KICMS+LEFEASRDG+   PRE          DSR WQ+ Y N +++LEVTY SFT IEQEWKLAQ+++E AS+ LYSATNEL I SLKAKSASGDLQST+L+MRDC  EASVALS+F  VSRG TALT+ESG+ML+EVLA TEDLHDVH+LGKEAA++HRSLM+DLSKANAIL PLES L+KD + + EAM RE +T +EVS  HGQAIYQSYG+++RE+ Q  +PLVPS+  SVK
Sbjct: 2346 LQEHHDLLLATLPAVELSLELNDGEIVFQRFSKVLNQYEIASSVFLQADQERSKLILRETSAKKTVAEAACNSENIRASFEIQAQEFSQVKALVSEKAQESLVWMEQRGRILGALRRNLIPEITAPTVLTSKLGSLSLTSAVLVAGVRLTVVPEPTQVQCNNIDTEISLLVNDLSNGLSSALVALQTYSLALQRILPLNYHTTSQVYDWVQVLQLAAHALSTDILSLAKRQAGEQFAKLQGGDSHSVSNCYDDLCCKVEKYADDLKKVEGEYAELAASVGMGPEFKAKDRLFYGLINYMQSPAFVESTNAGFS-----NKFEATEGVRLQNS-GKKTSKSLALLHTSISSFYDRLKEQVHYILNASVERKERSESFVSESRSLSTNLEAQVEMCMILVDFLNEVEYCVGQEISTVEESLTGSAHRVKENWALIFHRTLLSSKTLIAHMTEDVVPDVLKTYLVNNSDFMDAFGLISQIRGSIDTALEHLIEIKVERDSLVELQQDYFKKVSHITEGQLALEKAALKSREHLSWEEAEEFSSQGEACRTQMDQLNQSWGQREFQILSSLIKKEAQVKNALISAEKQFQLLTNANESNKPNDLKSSGILVELVKPFSELKQLDKTLSSLSSSAVSMSDLIPAFGDLLGCGKSLSENMSLFRSVLKDHSFFIWKVCIIYSFLDSCIHDATPSADQTLGFEQLILLMKKKFEFQLQERVDCYLAGSVAPAFLSQLDKENERLKHFSE-ENSVSGDQVKPESSHLKQVHTMLEEYCNAHETASAAKSAASRMKEQIKEIRDALRRTSLDIVQMEWMNDTTLTPSQNIRTTLQQSLASDDNLHPIFLDLNRAKLLESIRSAIPQITRSIERLQACEQNSITAEGQLERAMGWACGG------SSSSAKMSGIPTEFHDHLSRRRQLLWDAREKASNIAKICMSLLEFEASRDGVCLNPREALERDAKIRGDSRAWQETYQNLVSRLEVTYKSFTDIEQEWKLAQASVEAASTALYSATNELSITSLKAKSASGDLQSTILSMRDCTREASVALSSFIRVSRGQTALTTESGAMLKEVLATTEDLHDVHSLGKEAATLHRSLMDDLSKANAILTPLESTLSKDDALIAEAMTRESKTNIEVSSKHGQAIYQSYGVKIRESYQNLRPLVPSIVTSVK 3500          

HSP 3 Score: 119.783 bits (299), Expect = 4.687e-23
Identity = 51/69 (73.91%), Postives = 60/69 (86.96%), Query Frame = 3

Query: 11670 PSTDTASRIARGKNAYAMSVLRRVEMKLDGRDITERREISIAEQVDYLIKQATSVDNLCSMYEGWTPWI 11876
            P T   +RI RGKNAYA+SVL+ +EMK+DGR+I + +EISI EQVDYLIKQATSVDNLC+MYEGWTPWI
Sbjct: 3526 PPTQAHTRITRGKNAYALSVLKSMEMKIDGRNIADNKEISIPEQVDYLIKQATSVDNLCNMYEGWTPWI 3594          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy TrEMBL
Match: U5D8D2_AMBTC (Uncharacterized protein OS=Amborella trichopoda GN=AMTR_s00059p00199900 PE=4 SV=1)

HSP 1 Score: 2578.51 bits (6682), Expect = 0.000e+0
Identity = 1380/2335 (59.10%), Postives = 1744/2335 (74.69%), Query Frame = 2

Query:  293 RLAAINSLHRAIIYPPNSILVAHSASFLAQGFSQLLSDKSYAVRQSXXXXXXXXXXVVCSVPIGSSGRQNHVMLSSLVDRFIGWALPLLSNISAGDGTT-ELALEGLREFLSVGDVGAIERYALPILKACQELLEDERTXXXXXXXXXXXXXXXXXXXXXXFQPHFLDIIDLLLGWALVPDLAESDRRVIMDSFLQFQKHWVGNLQFSLGLLSKFLGDMDVLLQDGTHGTPQQFRRLLALLSCFSTVLQSTASGLLEMNLLEQISEPLSKMLPRLLGCLSVVGKKFGWSRWIEDSWKCLTLLAEILQERFSTFYPLAIDILFQSLELGNTSRPIGAGKITSFQVHGVXXXXXXXXXXXXXXXXPSSVKKILHFDRAISQLRLHPNHLVTGSSAATYVFLLQQGNDEIVQQAXXXXXXXXXXXXXXXXXXI------VHGEEDNSAGDLRCYSKFELFALIKFDLKVLLTSVSLCGCNNLIVQPKIAALYLQRSESLISFIIEKLSPFESPIQFCVELQVHVIKTLDRLSMVKFFSKCSIRNQSGNIPVGDAATEKVLDGNSLRDVHPAVIIEYLREFGTLLVRALHVSSPVAVKIVALEWVQRFCENLISICESPSMR--YN----FYEEFGYVSLFGDLIFSILEAAFDREPKVRLHVTLSLELLLQARLMHPLYFNSVSDVVLEKLGDPDADIRNAYVRLLSHVLLTTMYVYGI--HDIGACSNSRPRALMLGNSSNLYWKQVFALKQLPQQLHSQQLVSILSYISQRWKVPLSSWIQRLIHTC-RSSKDSSLGQLEETGIVGANDLWLDIKMEN-ILEKFCSVNNLAGAWWAIHEAARYCISTRLRTNLGGPTQTFAALERMLLDVSHVLQLDGEQNDGSLSIIGSSGAHLLPMRLLLDFVEALKKNVYNAYEGSAVLPSAGRQSSLFFRANKKVCEEWFSRICEPMMNAGLALQCHDATIQYCTLRLQELKNLVVSAFKEKSQAQVTEYLHNMREKYIGDISRIVRHMSLALCRNHESEALIGLQKWVSLTFSPLLLDEDQ-SVNHSGIVEPFQWITGLVYQAEGQYEKAVSHFAHLLQTEESLSTMGSDGAQFCIARIIESYTAVSDWKSLESWLLELQTLRAKYAGKSYSGALTTAGNEMNAIHALAQFDEGDLQAAWAYLDLTPKSSSELTLDPKLALQRSEQMLLQALLFKIEGNLDKVPHELQKAKSMLEEMLSVLPLDGLAEATACVTQLHCIFAVGEGYDLSQGNCWKHMGSQGKSKLNQ--SGLGSYLLPLQPLIKGIHQDCNPWLKVLRVYRTISPTSPVTLKLSMNLMSLARKQGNLTLANCLINYLRDHVSSCSHERYCNLLNLNLQYEGILILHAENKIEDAFANIWSFLRPYLCSSSFIVNDVDDGLMKAKACLKLSNWLRQNYSSLNLRNIVLRMLSDLNVATVSS-SGTGGYSFSDENLSSKWSLDVIVEEIVGTATKLSTQLCPKMAKSWISYASWCFNQAKSSVANQQEKCLQSCSFSPILVSELTPKRFKMTEDEIQIVESVILPLF---QKRDSAEHVDDGAEQWGFCSGSAENLRTNNMLMTLVQQLVDIMEAAAGAPGTENSGGERXXXXXXXXXXXXXQHANFGIEETHLTYVIDKLIDIWWSLRRRRVSLFGHAAHAFIKYLLYSSTKL-SDGRLSGDFCQSQKQKVGSYTLRATLYVMHVLLNYGLELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLVDINAYEEKPSEELQHILGCLRELYPRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKPETPHELWFHHEYKEQLKSAIVSLKTPPASAAALGDVWRPFDHIAASLASYQRKSSISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKSSVPPVPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKGQRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEWTRGDFHDDAAIGGEERKGMELAVSLSLFASRVQEIRVPLQ 7222
            R++AINSLH AII+P N +LVA S+SFL+QG SQLLSDKSY+VR++A  AY +LC+VVCS  + S+G Q+  +   L +RF+ WALP   +IS  +G   ELA EGL EF   G+ G IE Y LP LKACQELLEDERTSL+LL +LL +LT++ +KFS SFQ HF+DI+DLLLGWALVPDL+ESDR +IM +FLQFQKHW  N QFSLGLL+KFLGDM+V+L DGT  TPQQFRRLLALLSCF TVLQ+TASG+LEMNLL +I EPL  M P+LL CLS++G+KFGWS+W+++S +CL LLA+IL+ERFS FY L +DILFQ+L   N        K+  FQVHGVLK NLQL+SLQKL L PS+VKK+L FD  +S+LRLHP+HLVTG  AATY+ LLQ G   +V QA+  L EEL LL+ +L+K +      +HG           YS+ EL ALIKFDLK+LL+SV L     L+ Q ++     +RS  L SFI EKL PF +P Q  ++LQVH +  L +LS V+F SK ++  QS      +          + +      I+ YLR++   +VRALH  SP+ VK+ A EW++ FC  ++++    +M   +N    FY+     S+  D + SIL+AA DRE KVR  V   LELLLQARL+ P  F SV+++ LEKLGDP+ D + A++R++S  L   +Y  G     +    +  P    L +  + +WK +FALKQLP QL SQQ V+ILSY+ QRWKVPLS+WI RL+ +  +SSKDS  GQLE++G +G + + +++K+E+ +LEK C  N LA AWW+IHEAAR+CI+ RLRT+LGGPTQTFAALERMLLD++H L+LD E  +G + IIGS   HLLPMRLLLDFVEALKKN YNAYEGS V+PSA  QS LFFRANKKVCEEWFSR+CEPMMNAGLALQCH A + Y + RL +L+N+VVS+ K+K +AQ+TE LH++R K  GD+ R++RH++L++CR  E +AL GLQKW   TF  +LLD+ Q     +G   PF WITGL+YQA GQYEKA +HF+ LLQ+EE+LS+MGSDG QF IAR +E +TA+SDWKSLE+WL+ELQ LRA++AGK Y GALTTAGNE+NAIHAL++FDEGD+  +W+YLDLTPKSS+ELT+DPK AL RSEQ+LLQA+L+K + N  K+  E++KAK ML+E LSVL LDGL EA     QLHCI+A  EG       C KH+     S   Q  + + S    +   I  +++DC+ W+K+LRV+R++SP S +TLKL   L++LARKQ N  LA+ L+  L  ++S  ++     L   NLQYE IL+++AE K EDA  ++WS L PY+ S   +V+D ++ +MKAKACLKLS+WL +    +N  NI L++  D     V+  S + G   SD N S      + +E+I G ATKLS+ LCP M KSW+SYASWC+N+AK  ++   ++ L+SC+  P L+ E++  +  +TE+E+  V +++  L    + R     VD+    W       EN +    +  LVQ+ + +++ AAGAPG+E+   E L S L+SQL  +   AN GIE +++   + +L++I++S+RRR+V LFGHAAH +++YL +S++K   DG   G      KQK  S  LRATLYV+HVLLNYG+EL+D LE  L+TVP L WQ++TPQLFARLSSHPE+VVRKQ+E LLM LAKL+P SIVYP LVDINAYE +PSEELQ ILGCL +L+P L++DVQ+VIN LG +TVLWEE WLSTLQDLH DV+RR+++LKEEAAR+AEN TLS SEK KINAAKYSAMMAP++VA+ERRLASTSR P+TPHE+WF  EY EQLKSAI + K PP S AALGDVWRPFD IAASLA++Q++SS+SL + APQLA LSSS+VPMPGLEKQ++ + SD    + LHGIVTI+SF E +TILSTKTKPKKL ++GSDG+ YTYLLKGREDLRLDARIMQLLQAIN FL S S     LL +RYYSVTPISGRAGLIQWVD+V+SIYS+FKSWQ+R+QLAQ++   + +  +++PPVPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEVKRKVL+DLMKE P+QLL++E+WCASEGFKAF+SKLKR+SGSVAAMSMVGHILGLGDRHLDNIL+DFS+GDVVHIDYNVCFDKGQRLK+PEIVPFRLTQT+EAALGLTGIEGTFRANCE+V+ VLRKNKD++LMLLEVFVWDPL+EWTRGD HD+A IGGEERKGMELAVSLSLFASRVQEIRVPLQ
Sbjct:   31 RISAINSLHHAIIHPHNLLLVARSSSFLSQGLSQLLSDKSYSVRRAAVTAYGSLCSVVCSPQMSSNGLQSPTVSGGLAERFMAWALPQFRDISIDNGAAAELAFEGLLEFFGSGEAGGIELYVLPTLKACQELLEDERTSLNLLRKLLGLLTIVSVKFSRSFQSHFIDIVDLLLGWALVPDLSESDRCIIMGTFLQFQKHWSVNSQFSLGLLAKFLGDMEVVLLDGTPVTPQQFRRLLALLSCFLTVLQATASGMLEMNLLAKICEPLQSMSPQLLSCLSMMGEKFGWSKWVKESCRCLMLLADILRERFSNFYILVVDILFQNLR-ENNGHSEEFHKVPLFQVHGVLKANLQLMSLQKLCLPPSAVKKLLCFDSPLSRLRLHPSHLVTGIIAATYLLLLQHGCRGVVDQALASLMEELNLLQSVLEKNLNCCDQFIHG-----------YSELELLALIKFDLKILLSSVCLNAPGGLLSQVEVVRSKHERSVKLASFIFEKLDPFRAPFQSFLDLQVHTLSVLRKLSEVEFLSKHTLNQQSRKTGTSNEIGSDFHSVVTFKKEISFEIVGYLRKYSGYIVRALHSMSPLTVKLEAFEWLRSFCGAVMAVLSDANMNPSFNQTDSFYKASWQASIGSDFLSSILDAASDREVKVRSQVASILELLLQARLISPECFYSVAELALEKLGDPNIDTKTAFLRVISLFLPMAIYTCGCLEDKVRNSFSPAPSNFRLRSRCHWHWKLIFALKQLPHQLTSQQFVTILSYVCQRWKVPLSNWILRLLFSSLKSSKDSFSGQLEDSGKLGHDGILMNVKLESKMLEKICLANCLAAAWWSIHEAARHCITVRLRTHLGGPTQTFAALERMLLDIAHNLKLDTEPGEGGI-IIGSPTVHLLPMRLLLDFVEALKKNTYNAYEGSYVMPSASLQSELFFRANKKVCEEWFSRMCEPMMNAGLALQCHSAVLHYSSSRLLDLRNVVVSSLKDKPRAQLTEILHSLRAKIGGDVLRVLRHLALSMCRCREPDALAGLQKWAVATFFSVLLDDTQHGSGPTGSFGPFSWITGLIYQAHGQYEKAAAHFSVLLQSEEALSSMGSDGVQFIIARALECFTALSDWKSLEAWLMELQALRARHAGKGYCGALTTAGNEINAIHALSRFDEGDIHGSWSYLDLTPKSSNELTVDPKQALHRSEQLLLQAMLYK-DVNEVKMAEEIEKAKLMLDEPLSVLSLDGLPEAVGYAVQLHCIYAFEEG-------C-KHLSIPIGSNPKQLPAMMSSLYQVVHYPINKVYEDCSLWIKILRVFRSVSPNSELTLKLFQQLITLARKQRNFMLAHRLLENLTSNLSVDANGALEGLFITNLQYERILLMNAEEKYEDAVRSLWSLLCPYILSPGNVVSDSNN-VMKAKACLKLSSWLLEKDPKINWENIYLKIREDYQSFRVTGISDSEGTGLSDTNSS------LFLEDIAGAATKLSSILCPTMGKSWLSYASWCYNRAKKYLS-ADDRVLESCTLLPTLLPEISLDQSGLTEEEVTKVNAIVRNLLLSSRVRKETNIVDEEVIVWADTEPPLENGKD---VKGLVQKAIHLIQTAAGAPGSESISCESLPSMLSSQLQQAFLTANIGIEHSYVLSSVRELVNIFFSVRRRKVLLFGHAAHGYLQYLSHSTSKFHEDGYSDGLHLDLTKQKQESCCLRATLYVLHVLLNYGVELRDMLEHGLATVPPLPWQEITPQLFARLSSHPEQVVRKQLEGLLMTLAKLTPWSIVYPTLVDINAYEGEPSEELQRILGCLDKLHPELVKDVQMVINGLGMLTVLWEEQWLSTLQDLHTDVIRRVSLLKEEAARVAENATLSVSEKAKINAAKYSAMMAPVIVAVERRLASTSRTPDTPHEVWFQKEYGEQLKSAIATFKRPPISTAALGDVWRPFDAIAASLATHQKRSSLSLSDAAPQLAHLSSSNVPMPGLEKQISLYGSDGDQRAELHGIVTISSFCEQVTILSTKTKPKKLGLIGSDGQKYTYLLKGREDLRLDARIMQLLQAINGFLSSGSNIRARLLAVRYYSVTPISGRAGLIQWVDDVVSIYSVFKSWQSRIQLAQMA--NSSNLGNTIPPVPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEVKRKVLVDLMKETPRQLLYREIWCASEGFKAFSSKLKRFSGSVAAMSMVGHILGLGDRHLDNILMDFSTGDVVHIDYNVCFDKGQRLKIPEIVPFRLTQTMEAALGLTGIEGTFRANCESVIGVLRKNKDIILMLLEVFVWDPLVEWTRGDGHDEATIGGEERKGMELAVSLSLFASRVQEIRVPLQ 2330          

HSP 2 Score: 1325.84 bits (3430), Expect = 0.000e+0
Identity = 769/1481 (51.92%), Postives = 1015/1481 (68.53%), Query Frame = 3

Query: 7509 LQEHHDLLLATFPAVESALERFRDVLNQYELVSALFYRADQERSNLILHETSAKSIVAEATCNSEKICASFEIQAREFKQAKNVVAEKAQQATTWIEQHGRILDALRGNLIPEISAGMNLSGMADALSLTSAVPEAGVPLTIVPEPTQAQCYDIDREVSQLIAELDRGLSSAVMALQAYSLALQRVLPLNYLTTSTVHGWAQVLQLSANAVSSDILSLARRQASELIAKVHGDNLEFMKSSH---DDLCFXXXXXXXXXXXXXXXXXXXXNSIGSKTESKAKDRLMFAFMKYMQSAGLVRKEDANSSLQYGESKY-DGTKASR-IREDLEEKKDKVLSVLSVAMRSLYDDVKHRILEIYSH-TNRAQIENSRP--QSDLGTVFSGFEEQVEKCILVAGFVNELWQQIGGDMPGVDRDLYYSKYYSEGNWASIFKTILNCCKSLIGEMTEVVLPDIMRSAVSFNTEVMDAFGLISQIRGSIDTALEQLVEVELERASLAELEQNYFVKVGLITEQQLALEEAAMKGRDHLSWXXXXXXXXXXXXCRAQLEQLHQTWNHRDMRTSSLIKREAEIKSSLVSCEQHFQSLITGDDFRESHRSKSQVLLAILVKPFSELESVDKAXXXXXXXFAPHSDEIPNLVEFLSSGHSVSECVWNFGSLLNNHSFFIWKIGVLDSILDLCIHDMASSVDQNLGFEQLFDVVKRKLEIQLQEYIGRYLKIRIAPALLSWLDKENEHLKLLTEGAKEPSNDHVRKDVEAVKKVQLMLEEYCNTHETARAARSAASVMKRQVNELKEALRKTILEIVQMEWMHDVGLTPSHTSRVLFQKFFSNDDELYPVVLNFSRPKLLETMQSVLSKLARAIEGLKSCEHTSIVAEGQLERAMGWACGGPNSSVAGNSS-TKASGIPPEFHDHLMRRRHLLQEAREKASSVVKICMSILEFEASRDGIFQIPREGYALSTGSDSRTWQQAYFNALTKLEVTYHSFTRIEQEWKLAQSNMEVASSGLYSATNELCIASLKAKSASGDLQSTVLAMRDCAYEASVALSAFAHVSRGHTALTSESGSMLEEVLAITEDLHDVHNLGKEAASVHRSLMEDLSKANAILLPLESVLAKDVSAMTEAMARERETKMEVSPIHGQAIYQSYGLRVREACQTFKPLVPSLTFSVKEXXXXXXXXXXXXXXXXGNLHKALEGLGESQEVKSQSISLSRPDLAS-DATEYDERXXXXXXXXXXXXPKDIV---GLTGLSLQ-----EKEWISPPDSIGTSGMESSITSNGTSLSDSINDPVVEMMEKISLDSSQKKDHGDPNFVPSSESEYDEISHCGHRMSENMEVKNTNEVKSANEETNENLKTVPSVNDEAVSAPLESSQPSNKVNL----DVKFQ---GKDEVSTLGKIEVGDESHEVPVPSTDTASRIARGKNAYAMSVLRRVEMKLDGRDITERREISIAEQVDYLIKQATSVDNLCSMYEGWTPWI 11876
            LQEHHDLLLAT PA ESALERF +V+N+YE+ SA FY ADQERS+L+LHE SAKS+VAEATCN+EK  A+FE+QAREF QAK V AE AQ+A  W++ HGR++DALR   IP++   + LS   +ALSL SAV  AGVPLT+VPEPT A C +ID E++QL AE D G   AV +LQAY++ALQR+LPLNY+TTS VH WAQ+LQ+S N +SSD+L+L RRQA++LIAK  GD+  F  S H   + +C K++KY  EI+KV EEC+EL  SI S+TE+K+KDRL+ AF KYM     +R+++  S L   +SK+ D TK  + +  ++EEK+ KVLSVL +A R +Y +VK ++L + S  T R  I +       +    FS  EEQ+EKC+LVAG VNE+    G   P    D     Y  +GNWAS F+T +  C+SLI +M + VLPD++RS +S++T VMDAFG +SQIRGS+DTA+EQL+EVELE+ SL +LE+NYFVKVGLITEQQLALEEAA+KGRD+LSWEEAEELA+QEEACRAQL+QLHQTWN +D   SSL +RE ++++SL   E+ F SL   +   + H  +S +LLA L   FSELES+D+ LSS  +  +    +  +  E + SG+S+++ +W    LL  HSFF+W+IG++DS  D CIHD+ +S D NLGF+QL+   K+KLE++LQ ++  YL+ R+ P LL  LDKE+E+L+L     K+   +  R+++  VK+   M EEYCN HETARAA++A S+MKR++ EL   L+K  LE VQ+EW+HD+GL     +R++   F  +D+ L P++L   R K+LE +Q  +S LARA + L++CE T+  AE  LERAMGWACGGP+SS       TK SGIP EFHDHL+ R+ LL  ARE+AS ++KIC S+LEFEASRDG F+IP E  A     D R WQQ YFNALT+L++T+HSFTR E +WKLAQS+ME A+SGL+SATNEL IA +KAKSASGD+Q  + +MRD  YEA  ALS+F  V+RGHTALT+E GSMLEEVLAIT+ + D++ LGKEAA+VH++LM DL+KAN+ILLPLES+L  DV+AM   ++RERE+K+E+  + GQA+YQ+Y L++RE+CQ  + LVPSL  SVKEL  L+T LAR+ASLHAGNLHKALEGLGESQ+ +SQ I LS   L   D    DE              K+ +   G +G ++      E EW+SPPDSI +S   S +TS   +     +DP           +S     G  + + SSE    +         + ME  N      A  E+     T P    ++   PL SSQP  + NL     V ++    K+EV T  +      + + P  + D +SR+ RGKN+YA+SVLRRVEMKLDGRDI   R++ IA  VD+LI+QATS+DNLC+MYEGWTPWI
Sbjct: 2329 LQEHHDLLLATIPAAESALERFAEVINKYEIASAFFYHADQERSSLLLHEASAKSVVAEATCNTEKTRATFEVQAREFAQAKAVAAENAQEAGLWVDHHGRVIDALRSGSIPDLQESLRLSSSGEALSLISAVQVAGVPLTVVPEPTLAHCSEIDGEIAQLSAEWDDGFHCAVNSLQAYAVALQRILPLNYVTTSKVHSWAQLLQVSVNNLSSDVLALTRRQAADLIAKAKGDS-TFDSSVHQRYEAICIKMDKYVKEIKKVREECSELEESIESETETKSKDRLLSAFTKYMPPTNQLRRDEDVSGLVLVQSKHNDETKDPQMVAVEIEEKRAKVLSVLHIAAREMYIEVKEKLLGLPSTITERPFIVSGEDGLHHNSKISFSELEEQIEKCVLVAGVVNEVQHFSGLKFPRRGFD-----YPLDGNWASGFRTSILACRSLIDQMIDSVLPDLIRSVISYDTAVMDAFGFLSQIRGSVDTAVEQLIEVELEKMSLMDLEENYFVKVGLITEQQLALEEAAVKGRDNLSWEEAEELATQEEACRAQLDQLHQTWNQKDAHASSLTRRETQLRNSLNLSEKRFSSLTNFEQGGDMHVMRSNILLAALANSFSELESIDRMLSSFGTGESYSKTKPLSQAELVRSGYSLTDLIWKDVHLLKEHSFFVWRIGIIDSFFDSCIHDLTASADHNLGFDQLYSAQKKKLELKLQAHLDCYLRERVVPVLLDTLDKESEYLQLTIPETKDVGPNQPRREIGTVKRAHAMFEEYCNAHETARAAKAAVSLMKRRLRELSLNLQKACLEAVQLEWLHDLGLPYVQETRLILSGFL-DDNSLSPMILELKRHKILEDIQVGMSSLARATDSLQACERTAGSAEEPLERAMGWACGGPSSSSGTGIGSTKVSGIPSEFHDHLITRKQLLWAAREQASGIIKICSSLLEFEASRDGHFRIPGEASAGRAPDDGRVWQQVYFNALTRLDITFHSFTRAEHDWKLAQSSMEAATSGLFSATNELSIALVKAKSASGDIQGVLTSMRDSTYEAGGALSSFGRVTRGHTALTTECGSMLEEVLAITDGIPDIYGLGKEAATVHKALMVDLTKANSILLPLESMLVSDVAAMANVISRERESKLELPLVQGQALYQTYCLKLRESCQPLRSLVPSLLHSVKELLILVTNLARSASLHAGNLHKALEGLGESQDGRSQGIVLSSSKLGGHDIFSIDED-------------KNFIRNEGASGYTVDDDFCPEDEWVSPPDSIYSSSPRSGVTSTENATIGGTSDP----------SNSASSFIGGVHEILSSEKPETQ---------QYMEALNDGISSLAITES-----TSPPNTSDSQLKPL-SSQPDREYNLAEDISVNYESLGNKNEVVTTEQDNGRGGNSDDPPSNADPSSRVPRGKNSYALSVLRRVEMKLDGRDIDGDRQLDIAAHVDHLIRQATSIDNLCNMYEGWTPWI 3764          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy TrEMBL
Match: M0SJ91_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1)

HSP 1 Score: 2565.8 bits (6649), Expect = 0.000e+0
Identity = 1394/2344 (59.47%), Postives = 1726/2344 (73.63%), Query Frame = 2

Query:  284 DSARLAAINSLHRAIIYPPNSILVAHSASFLAQGFSQLLSDKSYAVRQSXXXXXXXXXXVVCSVPIGSSGRQNHVMLSSLVDRFIGWALPLLSNISAGDGTTELALEGLREFLSVGDVGAIERYALPILKACQELLEDERTXXXXXXXXXXXXXXXXXXXXXXFQPHFLDIIDLLLGWALVPDLAESDRRVIMDSFLQFQKHWVGNLQFSLGLLSKFLGDMDVLLQDGTHGTPQQFRRLLALLSCFSTVLQSTASGLLEMNLLEQISEPLSKMLPRLLGCLSVVGKKFGWSRWIEDSWKCLTLLAEILQERFSTFYPLAIDILFQSLELGNTSRPIGAGKITSFQVHGVXXXXXXXXXXXXXXXXPSSVKKILHFDRAISQLRLHPNHLVTGSSAATYVFLLQQGNDEIVQQAXXXXXXXXXXXXXXXXXXIVHGEEDN--------------SAGDLRCYSKFELFALIKFDLKVLLTSVSLCGCNNLIVQPKIAALYLQRSESLISFIIEKLSPFESPIQFCVELQVHVIKTLDRLSMVKFFSKCSI-RNQSGNIPVGDAATEKVLDGNSLRDVHPA--VIIEYLREFGTLLVRALHVSSPVAVKIVALEWVQRFCENLISICESPSMRYNFYEEFGYVSLFGDLIFSILEAAFDREPKVRLHVTLSLELLLQARLMHPLYFNSVSDVVLEKLGDPDADIRNAYVRLLSHVLLTTMYVYGIHDIGACSNSRPRALMLGNSSNLYWKQVFALKQLPQQLHSQQLVSILSYISQRWKVPLSSWIQRLIHTCRSSKDSSLGQLEETGIVGANDLWLDIKMENI-LEKFCSVNNLAGAWWAIHEAARYCISTRLRTNLGGPTQTFAALERMLLDVSHVLQLDGEQNDGSLSIIGSSGAHLLPMRLLLDFVEALKKNVYNAYEGSAVLPSAGRQSSLFFRANKKVCEEWFSRICEPMMNAGLALQCHDATIQYCTLRLQELKNLVVSAFKEKSQ-AQVTEYLHNMREKYIGDISRIVRHMSLALCRNHESEALIGLQKWVSLTFSPLLLDEDQSV--NHSGIVEPFQWITGLVYQAEGQYEKAVSHFAHLLQTEESLSTMGSDGAQFCIARIIESYTAVSDWKSLESWLLELQTLRAKYAGKSYSGALTTAGNEMNAIHALAQFDEGDLQAAWAYLDLTPKSSSELTLDPKLALQRSEQMLLQALLFKIEGNLDKVPHELQKAKSMLEEMLSVLPLDGLAEATACVTQLHCIFAVGEGYDLSQGNCWKHMGSQGKSKLNQSGLGSYLLPLQPLIKGIHQDCNPWLKVLRVYRTISPTSPVTLKLSMNLMSLARKQGNLTLANCLINYLRDHVSSCSHERYCNLLNLNLQYEGILILHAENKIEDAFANIWSFLRPYLCSSSFIVNDVDDGLMKAKACLKLSNWLRQNYSSLNLRNIVLRMLSDLNVATVSSSGTG-GYSFSDENLSSKWSLDVIVEEIVGTATKLSTQLCPKMAKSWISYASWCFNQAKSSVANQQEKCLQSCSFSPILVSELTPKRFKMTEDEIQIVESVILPLFQKRDSAEHVDDGAEQWGFCSGSAENLRTNNMLMTLVQQLVDIMEAAAGAPGTENSGGERXXXXXXXXXXXXXQHANFGIEETHLTYVIDKLIDIWWSLRRRRVSLFGHAAHAFIKYLLYSSTKLSDGRLSGDFCQSQKQKVGSYTLRATLYVMHVLLNYGLELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLVDINAYEEKPSEELQHILGCLR---------ELYPRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKPETPHELWFHHEYKEQLKSAIVSLKTPPASAAALGDVWRPFDHIAASLASYQRKSSISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKSSVPPVPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKGQRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEWTRGDFHDDAAIGGEERKGMELAVSLSLFASRVQEIRVPLQ 7222
            D+AR+AA+ SL R I+YPPN+ +V+HS+ FL+QGF QLLSDKSYAVR++AAI Y +LCA++ SVPIGS+G QNHV+  +L +RF+ WALPLL      +G+ ELALE LREFL+VGD  +IER+   ILKACQELLEDERTSL LLH+LLSVLTLI LKF   FQPHF+DI+DLLLGWA +PDL+ES R +IMDSFLQFQKHW+ NLQFSL LLSKFLGD++VL+QD +  +  Q  RLLAL  CFSTVL+ TASG++EMN+LE + EPL  M PRLL C++  G+K G  +W+ +SW+CL LLAEIL E FS  YP+ IDILFQ+L        IG   I S Q++ ++KTNLQLLS QKL L PSSV+ +L F   +SQLRLHPNHLV  ++AATY+F LQ G++ +V QA++ L EELELLKGML K   H    N                G  + YS+ EL +LIKFDLK LL+S+      +L  Q  I +L   RS  L S I+EKL PFESP+    ELQV ++KTL+RLS V+  SK +I R     + V          G S R +     +I EY R++   +VRAL V+SP++VK+ AL+W+  F   + ++     +   F +      +  DL+ +I +AA+ RE KVR  V   LE LLQARL+   YFN VS+V L+KLGDPD  ++NA+ R+LS VL  TMY  G++D  +         +  N+  L WK   ALKQ  ++LHS QLVSI+SYISQRWKVPLSSWIQRL+ +C   KDS   + E  G  G  D+W   K E   ++K C VNN+A  WW+IHEAAR+CI+ RLRTNLGGPTQTFAALERMLLD+ +VL LD EQ D     I SS +HLLPMRLLLDFVEALKKNVYNAYEGS +LP+  RQSS+FFRANKKVCEEWFSRICEPMMNAGLAL C+DATIQYC LRLQ+LK+L  S FKEK++ A V+E  H +R ++ GD+ ++VRH SLALCRN+E EALIGLQKWVS  FS +L  ED+ +    +G    F WI GLVYQA+GQYEKA ++F+HLLQ+E +L+ MGSDG QF IAR+IE +T+VSDW SLESWL ELQ+LRA +AGKSYSGALTTAGNE+NA+HALA++D GD QA+ +YLDLTPKSS E+ LDPK+AL+RSEQMLL+ L+ K +G++ K   EL+KAK ML+E LSV+PLDGL +A AC  QLHCI A  EG   S         +  ++K + S LGS    L   I  + QDC+ W KV RVYRT+ PTS  TL L   L+++ARKQ N  LA+ L  YLRDH+   S   + +LL L +QYE IL+ HA+   E+A  + WSF+   + S++ I +  D G+++AKACLKLS WLRQ + +L+ RN++L++  D +  +  +S  G G +  D  L +    + I+EEI+GTA K+S  LCP ++K+W+SYASWCF+QAK S        LQSCS S +L  EL+P+R K+TE E+  VE +I  +F   D   +V  GA++    S S        ++ +LV+Q   IM+ AAG+PG E+  GE  S+ ++SQL     + N G+ +  +   +D+L+ IWWSLR+RRVSLFGHAAH + +YL YSS  L+    + DF    K+K  S TLRA LYV+H+++NYG EL++TLE  L+ VPLL WQ++ PQLFARLSSHP++VVRKQ+E LL+MLAKL P S+VYP LVD+NAY+ +P EELQ IL CL          +LYP+LIQDVQLVINELG +T+LWEELWLSTLQDLH DV+RRIN+LKEEAAR+AEN TLS +EKNKINAAKYSAMMAPIVVALERRL STSR+P T HELWFH EY E+LKSAI+SLKTPP SA ALGDVWR FD I  SLA++ RKS +SL EVAP LA LSSSDVPMPG EKQ++  +S  + T+++ GIVTI+SF E +TILSTKTKPKKL++ GSDG+ YTYLLKGREDLRLDARIMQLLQAINS L SS+ T    L IRYYSVTPISGRAGLI+WVDNV SIYS++KSWQ   Q+AQVSA+ AG+  + +PPV RPSDMFYGKIIPALKEKGIRRVISRRDWP EVKRKV L+LMKE P++LL QE+WCASEGF+AF  K KR+SGSVAAMSMVGHILGLGDRHLDNIL+DFS+G++VHIDYNVCFDKG+RLKVPEIVPFRLTQTIE ALGLTG EGTFR+NCEAV+S+LRKNKD++LMLLEVFVWDPL+EWTRGD HD+AAIGGEE+KGMELAVSLSLFASRVQE+RVPLQ
Sbjct:   54 DAARIAALESLQRIILYPPNAFVVSHSSPFLSQGFYQLLSDKSYAVRRAAAITYGSLCAILVSVPIGSNGPQNHVL--ALAERFMAWALPLLGETDVQNGSAELALESLREFLNVGDANSIERFVPLILKACQELLEDERTSLGLLHQLLSVLTLISLKFGHCFQPHFVDIVDLLLGWAFMPDLSESGRTIIMDSFLQFQKHWLSNLQFSLELLSKFLGDIEVLIQDTSLESGSQLGRLLALFFCFSTVLRVTASGMMEMNILEDVREPLENMAPRLLKCIASFGRKSGSPKWMGESWRCLILLAEILNEGFSKSYPIVIDILFQNL--------IG---IPSLQINALVKTNLQLLSRQKLALHPSSVRSVLGFYSPLSQLRLHPNHLVVANTAATYLFFLQHGSNAVVSQAISYLIEELELLKGMLGKIRGHFANVNELQLDFVTDDQLKLDLGSGKAYSEHELISLIKFDLKALLSSIPCGAAESLQDQVVIDSLRYDRSMRLASSILEKLHPFESPVDGWFELQVLIVKTLNRLSEVELLSKFTILRASWEKVSVNSV-------GKSERSLQAKTDLITEYKRKYAMCIVRALGVNSPISVKLEALDWICSFGWMIATLESDADLMMQFCDPHADAGVGSDLLVAIFDAAYVRESKVRSRVASVLEALLQARLIDHAYFNFVSEVALDKLGDPDVTVKNAFARVLSVVLPLTMYSRGLNDRASSICEFVHCTV--NNYYLSWKNELALKQCSRKLHSHQLVSIMSYISQRWKVPLSSWIQRLVFSCHCKKDSISSKEEIVGEPGNGDMWKGGKTEECAVDKICPVNNIAAVWWSIHEAARHCIALRLRTNLGGPTQTFAALERMLLDIPNVLLLDAEQTDSKY--IASSNSHLLPMRLLLDFVEALKKNVYNAYEGSFILPACSRQSSVFFRANKKVCEEWFSRICEPMMNAGLALHCYDATIQYCLLRLQDLKSLATSVFKEKARGASVSESFH-LRSRFAGDVMKVVRHASLALCRNYEPEALIGLQKWVSTQFS-VLFKEDKHIVSGVAGNFGQFSWIAGLVYQAQGQYEKAAAYFSHLLQSEVALALMGSDGVQFVIARVIECFTSVSDWNSLESWLTELQSLRAMHAGKSYSGALTTAGNEINAVHALARYDLGDFQASRSYLDLTPKSSCEIALDPKIALERSEQMLLR-LMLKRDGSIHKAGEELEKAKLMLDEALSVVPLDGLHQAAACAIQLHCILAFEEGTRSS---------NHDQAKRSPSLLGSLQQVLLTPISRVCQDCSLWTKVFRVYRTLMPTSLTTLLLCQKLLTVARKQNNFMLADRLSQYLRDHIRMTSEGSHSDLLALKMQYEDILLKHAKGNHEEAILDSWSFIGDNMRSTATISSGAD-GVLRAKACLKLSTWLRQGFPNLDFRNVLLKIRQDFSGCSKCTSFAGTGLTSGDGVLITDPDYNQILEEIIGTARKISCNLCPTLSKTWLSYASWCFDQAKGS-PPAGGAVLQSCSLSSVLCPELSPERCKLTEAEMSEVEIIIRKIFNS-DRDVNVASGADEDR--SESIYFQENEPLVSSLVEQAAYIMQTAAGSPGFESYDGECPSAAVSSQLQVLFLNTNGGMRKHDILPFVDELVGIWWSLRQRRVSLFGHAAHGYFQYLSYSSNLLACS--ANDF---PKEKNKSCTLRAMLYVLHIIVNYGYELRETLEHGLAAVPLLPWQELIPQLFARLSSHPKQVVRKQLEGLLLMLAKLCPWSVVYPTLVDLNAYDGQPLEELQRILDCLVIEPPLQLSFKLYPKLIQDVQLVINELGMITILWEELWLSTLQDLHTDVIRRINMLKEEAARVAENPTLSDTEKNKINAAKYSAMMAPIVVALERRLTSTSREPRTSHELWFHEEYIEKLKSAILSLKTPPKSATALGDVWRQFDTITTSLATHHRKSCVSLSEVAPHLASLSSSDVPMPGFEKQISMLDSSGSSTTSIQGIVTISSFCEQVTILSTKTKPKKLILQGSDGQRYTYLLKGREDLRLDARIMQLLQAINSLLTSSNDTRGRALAIRYYSVTPISGRAGLIRWVDNVTSIYSVYKSWQVHTQMAQVSAVDAGNMNNPMPPVLRPSDMFYGKIIPALKEKGIRRVISRRDWPLEVKRKVFLELMKETPRELLWQEMWCASEGFRAFTLKAKRFSGSVAAMSMVGHILGLGDRHLDNILMDFSTGEIVHIDYNVCFDKGRRLKVPEIVPFRLTQTIETALGLTGTEGTFRSNCEAVISILRKNKDIMLMLLEVFVWDPLVEWTRGDIHDEAAIGGEEKKGMELAVSLSLFASRVQEMRVPLQ 2351          

HSP 2 Score: 1254.97 bits (3246), Expect = 0.000e+0
Identity = 729/1480 (49.26%), Postives = 966/1480 (65.27%), Query Frame = 3

Query: 7509 LQEHHDLLLATFPAVESALERFRDVLNQYELVSALFYRADQERSNLILHETSAKSIVAEATCNSEKICASFEIQAREFKQAKNVVAEKAQQATTWIEQHGRILDALRGNLIPEISAGMNLSGMADALSLTSAVPEAGVPLTIVPEPTQAQCYDIDREVSQLIAELDRGLSSAVMALQAYSLALQRVLPLNYLTTSTVHGWAQVLQLSANAVSSDILSLARRQASELIAKVHGDNLEFMKSSHDDLCFXXXXXXXXXXXXXXXXXXXXNSIGSKTESKAKDRLMFAFMKYMQSAGLVRKEDANSSLQYGESKYDGTKASRIREDLEEKKDKVLSVLSVAMRSLYDDVKHRILEIYSHTNRAQI-----ENSRPQSDLGTVFSGFEEQVEKCILVAGFVNELWQQIGGDMPGVDRDLYYSKYYSEGNWASIFKTILNCCKSLIGEMTEVVLPDIMRSAVSFNTEVMDAFGLISQIRGSIDTALEQLVEVELERASLAELEQNYFVKVGLITEQQLALEEAAMKGRDHLSWXXXXXXXXXXXXCRAQLEQLHQTWNHRDMRTSSLIKREAEIKSSLVSCEQHFQSLITGDDFRESHRSKSQVLLAILVKPFSELESVDKAXXXXXXXFAPHSDEIPNLVEFLSSGHSVSECVWNFGSLLNNHSFFIWKIGVLDSILDLCIHDMASSVDQNLGFEQLFDVVKRKLEIQLQEYIGRYLKIRIAPALLSWLDKENEHLKLLTEGAKEPSNDHVRKDVEAVKKVQLMLEEYCNTHETARAARSAASVMKRQVNELKEALRKTILEIVQMEWMHDVGLTPSHTSRVLFQKFFSNDDELYPVVLNFSRPKLLETMQSVLSKLARAIEGLKSCEHTSIVAEGQLERAMGWACGGPNSSVAGNSSTKASGIPPEFHDHLMRRRHLLQEAREKASSVVKICMSILEFEASRDGIFQIPREGYALSTGSDSRTWQQAYFNALTKLEVTYHSFTRIEQEWKLAQSNMEVASSGLYSATNELCIASLKAKSASGDLQSTVLAMRDCAYEASVALSAFAHVSRGHTALTSESGSMLEEVLAITEDLHDVHNLGKEAASVHRSLMEDLSKANAILLPLESVLAKDVSAMTEAMARERETKMEVSPIHGQAIYQSYGLRVREACQTFKPLVPSLTFSVKEXXXXXXXXXXXXXXXXGNLHKALEGLGESQEVKSQSISLSRPDLASDATEYDERXXXXXXXXXXXXPKDIVGLTG---LSLQEKEWISPPDSIGTSGMESSITSNGTSLSDSINDPVVEMMEKISLDSSQKKDHGDPNFVPSSESEYDEISHCGHRMSENMEVKNTNEVKS--ANEETNENLKTV-PSVNDEAVSAPLESSQPS--NKVNLDVKFQGKDEVSTLGKIEVGDESHEVPV-----------PSTDTASRIARGKNAYAMSVLRRVEMKLDGRDITERREISIAEQVDYLIKQATSVDNLCSMYEGWTPWI 11876
            LQEHHDLL+ T P VESAL+ F DVLNQYE+ S  FY AD+E+S L+ HETSAK++V EAT   EK  ASFE+Q  EF Q+K V AEKAQ+A  WI+QHGR+LDALR   IP+    + LSG  +ALSLTSAV  +GVPLTIVPEPTQAQCYD+D+EVS LI ELD GLS A+ AL  Y+LALQ+VLP +Y+T S V+GWAQVLQLSAN++SS+ L LAR QA+ELIAK  G+  +  +  H DL  K+E  A EI K+  EC+ L+NSIG+ TE+KAK+RL+ +F KYMQ+AGL                          EDL EKK KV SVL +A+  LY ++  +++ IYS     ++     EN   ++D GT   GFEEQ+EKC+LVA F++E+ + +   +P        +   S   WAS+++  L     LI +MTE++LP+I+RSAV++N+E M+AFG++SQIRGS+DTALE+LVEVELE+ASL ELE+NYFVK                                        L+QLHQTWN +DMR SSL K EA I +SL+S +Q+F SL+  +D  + H  +S+ LLA L KPF+ELESVD                     E +SS   VSE VW    LL +H+FFIWK+ ++DS+LD+C HD++SSVD N+ F+QL+  +K+KLE+ LQE +G YL  R+ PA L+ L+KE E+L+ L E  +E + D  +KD  AV+K++LMLEEY N HET RAARS  S MKRQVNEL EAL KTILEIVQ+EW+HD+       S+V F      DD++ P+++N SRPKLLE +QS +S +AR++E L++CE TS  AEGQLERAM WAC G  +   G S+ K SGIP EFHDHL RRR LL   +E+A  ++KIC S++EFEASRDG+F IP E  +  T +D RTWQQAY N LT+L+V YHSF R EQEWKLA+SNME A++ L++ATNELCIAS+KA SAS DLQ T+  MR+ AYEAS AL+AF  VS+GHTALTSE GSMLEEVLAI E + DV+ LGKEAA+ H +LM DLSK N ILLPLE+ L+ D++ M +A  ++ E   E+S +HGQA+YQSY  ++REAC +  PLVPSLT  VKELHS LT LAR +SLHAGNLHKALEGLGESQ ++SQ +++S  + ++ A  +++       +S     +D+  LT    L+L ++ W+SPP+   TS  +S I+ +  S S                   +K D+ + +   +S  E   +S   +++ +  E K+  +  S  + E+   +L +V P+   +++ A      P+  N    D++ +GK  V+    +  G+E +   V                 SR  RGKNAYA+SVL++VE+KLDGR I + R + + EQVD L+KQAT+VDNLC+MYEGWTPWI
Sbjct: 2350 LQEHHDLLVTTVPTVESALKAFLDVLNQYEVTSTFFYHADKEKSRLMQHETSAKTVVTEATSMYEKTRASFEVQVNEFAQSKAVAAEKAQEAAMWIDQHGRVLDALRSGSIPDAKGLLMLSGTEEALSLTSAVVLSGVPLTIVPEPTQAQCYDLDKEVSHLIDELDSGLSCAIEALNEYALALQKVLPHSYITNSPVNGWAQVLQLSANSLSSEALLLARNQAAELIAKSTGEGYDSARQRHQDLLHKIELCAMEIGKITAECSVLMNSIGTDTEAKAKERLLSSFTKYMQAAGL-------------------------PEDLVEKKVKVFSVLCMAVIELYKEIIAKMV-IYSDNFTQKVLWRTRENGS-RADSGTTLVGFEEQIEKCVLVAVFLSEVQELLDITLPCTSTSAEDTNPSSAATWASVYQVCLCSSNQLIEQMTEILLPEIIRSAVTYNSETMEAFGMLSQIRGSVDTALEKLVEVELEKASLLELEKNYFVK----------------------------------------LDQLHQTWNQKDMRNSSLKKLEANIINSLMSSQQYFTSLMNSEDEGDLHVRRSKALLAALAKPFAELESVDH--------------------ELVSS---VSESVWGLRFLLRDHAFFIWKVSIMDSVLDMCFHDISSSVDHNVSFDQLYKSLKKKLEVHLQELLGHYLNGRVVPAFLAQLNKEIENLQHLMEARREFAPDQAKKDAGAVRKIRLMLEEYSNAHETVRAARSTVSFMKRQVNELTEALGKTILEIVQLEWLHDMSSLYLLKSKV-FSGNILGDDKISPLIINLSRPKLLEKLQSSMSSVARSLECLQACERTSTSAEGQLERAMAWACAGSTAVGTGTSTVKTSGIPTEFHDHLRRRRQLLWAIQEQAGDIIKICNSVMEFEASRDGLFWIPGEKTSGRTTADGRTWQQAYLNTLTRLDVAYHSFNRAEQEWKLAESNMETAANALFAATNELCIASVKANSASDDLQDTLAIMRERAYEASTALTAFVCVSKGHTALTSECGSMLEEVLAIMEGVEDVYILGKEAAAAHSALMADLSKVNMILLPLEASLSTDLTVMADASVKDEENNKEISLVHGQALYQSYIFKLREACPSLVPLVPSLTSYVKELHSTLTKLARVSSLHAGNLHKALEGLGESQILRSQDLAISSSEPSNGAVLFNDEEKVLSGSSV----EDVENLTTNGKLALHDEGWLSPPEHTYTSSPDSIISLSEGSFS-------------------EKSDNLEQDLHSNSAGEDTSMS-VSYKIIDGSESKSVEDQDSNYSTEDVANSLSSVLPADLGDSLQALSLCDGPTVENVGTYDIE-KGKSVVAN--SLMSGNEHYSNLVNGHGDNLDDSSSCFGAISRTTRGKNAYAISVLKQVELKLDGRGIEDIRSLEVPEQVDLLLKQATNVDNLCNMYEGWTPWI 3711          

BLAST of g.barbadense_cottongen_reftransV1_0095004 vs. ExPASy TrEMBL
Match: A0A059AN70_EUCGR (Uncharacterized protein (Fragment) OS=Eucalyptus grandis GN=EUGRSUZ_I012192 PE=4 SV=1)

HSP 1 Score: 2466.8 bits (6392), Expect = 0.000e+0
Identity = 1215/1841 (66.00%), Postives = 1481/1841 (80.45%), Query Frame = 2

Query: 1703 NLIVQPKIAALYLQRSESLISFIIEKLSPFESPIQFCVELQVHVIKTLDRLSMVKFFSKCSIRNQSGNIPVGDAATEKVLDGNSLRDVHPAVIIEYLREFGTLLVRALHVSSPVAVKIVALEWVQRFCENLISICESPSMRYNFYEEFGYVSLFGDLIFSILEAAFDREPKVRLHVTLSLELLLQARLMHPLYFNSVSDVVLEKLGDPDADIRNAYVRLLSHVLLTTMYVYGIHDIGACSNSRPRALMLGNSSNLYWKQVFALKQLPQQLHSQQLVSILSYISQRWKVPLSSWIQRLIHTCRSSKDSSLGQLEETGIVGANDLWLDIKME-NILEKFCSVNNLAGAWWAIHEAARYCISTRLRTNLGGPTQTFAALERMLLDVSHVLQLDGEQNDGSLSIIGSSGAHLLPMRLLLDFVEALKKNVYNAYEGSAVLPSAGRQSSLFFRANKKVCEEWFSRICEPMMNAGLALQCHDATIQYCTLRLQELKNLVVSAFKEKSQAQVTEYLHNMREKYIGDISRIVRHMSLALCRNHESEALIGLQKWVSLTFSPLLLDEDQSVNHSGIVEPFQWITGLVYQAEGQYEKAVSHFAHLLQTEESLSTMGSDGAQFCIARIIESYTAVSDWKSLESWLLELQTLRAKYAGKSYSGALTTAGNEMNAIHALAQFDEGDLQAAWAYLDLTPKSSSELTLDPKLALQRSEQMLLQALLFKIEGNLDKVPHELQKAKSMLEEMLSVLPLDGLAEATACVTQLHCIFAVGEGYDLSQGNCWKHMGSQGKSKLNQSGLGSYLLPLQPLIKGIHQDCNPWLKVLRVYRTISPTSPVTLKLSMNLMSLARKQGNLTLANCLINYLRDHVSSCSHERYCNLLNLNLQYEGILILHAENKIEDAFANIWSFLRPYLCSSSFIVNDVDDGLMKAKACLKLSNWLRQNYSSLNLRNIVLRMLSDLNVATVSSSGTGGYSFSDENLSSKWSLDVIVEEIVGTATKLSTQLCPKMAKSWISYASWCFNQAKSSVANQQEKCLQSCSFSPILVSELTPKRFKMTEDEIQIVESVILPLFQKRDSAEHVDDGAEQWGFCSGSAENLRTNNMLMTLVQQLVDIMEAAAGAPGTENSGGERXXXXXXXXXXXXXQHANFGIEETHLTYVIDKLIDIWWSLRRRRVSLFGHAAHAFIKYLLYSSTKLSDGRLSGDFCQSQKQKVGSYTLRATLYVMHVLLNYGLELKDTLEPALSTVPLLSWQDVTPQLFARLSSHPEEVVRKQIESLLMMLAKLSPSSIVYPMLVDINAYEEKPSEELQHILGCLRELYPRLIQDVQLVINELGNVTVLWEELWLSTLQDLHMDVMRRINVLKEEAARIAENTTLSQSEKNKINAAKYSAMMAPIVVALERRLASTSRKPETPHELWFHHEYKEQLKSAIVSLKTPPASAAALGDVWRPFDHIAASLASYQRKSSISLGEVAPQLAMLSSSDVPMPGLEKQVAPFESDRALTSALHGIVTIASFSEHLTILSTKTKPKKLVILGSDGKAYTYLLKGREDLRLDARIMQLLQAINSFLHSSSATNHNLLGIRYYSVTPISGRAGLIQWVDNVISIYSIFKSWQNRVQLAQVSALGAGSAKSSVPPVPRPSDMFYGKIIPALKEKGIRRVISRRDWPHEVKRKVLLDLMKEVPKQLLHQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILLDFSSGDVVHIDYNVCFDKGQRLKVPEIVPFRLTQTIEAALGLTGIEGTFRANCEAVVSVLRKNKDXXXXXXEVFVWDPLIEWTRGDFHDDAAIGGEERKGMELAVSLSLFASRVQEIRVPLQ 7222
            N +V   ++ LYLQRSE+L  F++E L+P   PI+ C++LQ+ V+  L+RLS V+F SKC++ +     P+ D A+E  L   SL+    A+ +E+LR++  LLV++LHVSSP+AVK+ ALEWVQRFCE+++++ E+ + R   ++ F       +LIFS+L+AA D+EPKVR HV L LELLL+ARL+HP+ F+ ++  +LE+LGDP+ DI+  + +LL HVL   +Y  G+++ G    S+    + GN S+L+WKQ+FALKQLP+QL +QQLVSILSYISQRWKVPLS W+QRLIH+CR  +D+S  QLEE G +G    WLDI+++ N+LE+ CSVN LAGAWWAIHEAARYC+STRLRTNLGGP+QTFAALERMLLDV HVLQLD EQNDG+LS+I +SGAHLLPMRLLL+FVE+LKKNVYNAYEGSAVLPS  RQSSLFFRANKKVCEEWFSRICEPMMNAGLAL CH ATIQYC LRL EL++LV SA KE S++Q++E    +R ++  DI R+V HM+LALCR HE EAL+GLQ WVS+TFSPLL++E+Q ++ +  + P   ITGLVYQAEGQYEK+ +HF HLLQ EESLS+MGS G QF IARIIESY+A+SDW+SLESWL+ELQ LRA++AGKSY+GALTTAGNE+NAIHALA FD G+ QAAWA LDLTPKSSSEL+LDPK+AL RSEQMLLQA+L ++EG ++K+P ELQK+K MLEE LSVLPLDGL +A A  TQLHCI     G+ L         G Q +SK  QS L SY+ P+Q  +   HQDC PWLKVLRVYRTI P SPVT+ L MNL+ LARKQ N+ LA+ L +YL ++VS CS ER CN L LNL+YEG L+++AE K EDAF+++WSF+ P + SS+  V+D D+ ++KAKACLKLSNWL++ +S ++L  +  RM +D  +   S  G GG S + ENLSS+    +I+EEI+GTATKLST+LCP M KSWISY SWCFNQA++S+    E  L SCS S  LV E+ P R K+ +DE+  VES+IL    K   A  + D   +      SAENL   N++  L+QQ+V+I+E  AGAPG E+S  +RLS ++ S+L +   HAN G+EE  +  ++D+L+DIWWSLRRR VSLFG AAH FI+YL Y+S+   +G+L G   +S K K  SYTLRATLYV+H+LLNYG+ELK+TLEP+LS VPL  WQ+VTPQLFARLSSHPE+VVR+Q++S+LMML K SP SIVYP LVD+NA EEKPSEEL+H+LGCL +L+PRLIQDVQL+INEL NVTVLWEELWLSTL DLH DVMRRI++LKEEAARIAEN TLS SEK+KINAAKYSAMMAP+++ LERRLAST+RKP TPHE+ FH +Y EQLK+AI++ K PP SAA+L D WRPFD IAASL SYQRKSS+SLGEVAPQLA+LSSSDVP+PGLEK VA  E+D  LT  L  +VTI+SFSE +TI+STKTKPKK+ I+GSDG+ Y YLLKGREDLRLDARIMQLL AI+S LHSS  T  +L+G+R+YSVTPISGRAGLIQWVDNVIS+YS++K WQ R Q A + + G G+ K+  PPVPRPSDMFYGKIIPALKEKGIRRVISR+DWPHEVKRKVL+DLM+EVP+QLL+QELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNIL+DF SGD++HIDYN+CFDKG RLK+PEIVPFRLTQ IEAALGLTG+EG F+ANCEAV+ VLRKNKD+LLMLLEVFVWDPL+EWTRGDFHDDAAIGGEERKGMELAVSLSLFASRVQEIRVPLQ
Sbjct:  449 NHVVTANVSTLYLQRSETLACFLLEHLNPLNFPIEACLDLQLSVMMALERLSTVEFLSKCAMGDIYTQNPLVDVASEDFLTDASLKKRLSAITVEHLRKYCCLLVKSLHVSSPLAVKLAALEWVQRFCEHVLTMTENLNARTYLFDSFICTGDLRNLIFSVLDAASDKEPKVRSHVALVLELLLKARLLHPVNFSLIAQAILERLGDPEPDIKTIFTKLLCHVLPAMVYSSGLYEYGTSIMSKH---LKGNISSLHWKQLFALKQLPRQLQAQQLVSILSYISQRWKVPLSCWVQRLIHSCRRLRDTSPNQLEEIGSLGTCGAWLDIEVDDNVLEEICSVNVLAGAWWAIHEAARYCVSTRLRTNLGGPSQTFAALERMLLDVVHVLQLDSEQNDGNLSMINTSGAHLLPMRLLLEFVESLKKNVYNAYEGSAVLPSVTRQSSLFFRANKKVCEEWFSRICEPMMNAGLALNCHYATIQYCVLRLHELRSLVSSALKEPSRSQISETFQTIRSRFSEDILRVVHHMALALCRCHEPEALVGLQTWVSMTFSPLLVEENQFMDQNAALRPLACITGLVYQAEGQYEKSAAHFTHLLQNEESLSSMGSAGVQFAIARIIESYSALSDWRSLESWLVELQMLRARHAGKSYAGALTTAGNEVNAIHALAHFDAGEHQAAWASLDLTPKSSSELSLDPKVALLRSEQMLLQAMLLQVEGKIEKLPLELQKSKQMLEEALSVLPLDGLTDAAAYATQLHCILEFERGFTLR--------GGQDESKQFQSILSSYIHPIQHPLNLAHQDCYPWLKVLRVYRTIYPASPVTMNLCMNLLRLARKQQNIMLAHRLKSYLIENVSRCSEERLCNSLKLNLEYEGALLMYAEKKYEDAFSSLWSFVHPCMISSALKVSDADESILKAKACLKLSNWLQRKHSDISLEILSARMQADFEITDFSFIGKGGLSLNSENLSSQRDASLIIEEIIGTATKLSTRLCPFMGKSWISYGSWCFNQARNSLLKSHETALHSCSLSSFLVPEVQPDRLKLNDDEMMRVESMILQRVSKLGHAVGLKDRVGEKDVYLLSAENLSNVNLVKALLQQVVNIIEVTAGAPGAEDSASDRLSDSVVSRLRTFFLHANIGLEERDIVCLVDELVDIWWSLRRRNVSLFGCAAHGFIQYLSYASSNTCNGKLIGSGYESLKHKSSSYTLRATLYVLHILLNYGVELKETLEPSLSAVPLFPWQEVTPQLFARLSSHPEQVVRRQLQSILMMLGKRSPWSIVYPTLVDVNANEEKPSEELEHVLGCLLDLHPRLIQDVQLMINELSNVTVLWEELWLSTLHDLHSDVMRRIHILKEEAARIAENITLSLSEKSKINAAKYSAMMAPVILTLERRLASTTRKPVTPHEMRFHEQYVEQLKNAILTFKNPPKSAASLADAWRPFDTIAASLTSYQRKSSVSLGEVAPQLALLSSSDVPLPGLEKLVALSEADGGLT-VLPSVVTISSFSEQVTIISTKTKPKKIYIIGSDGQNYPYLLKGREDLRLDARIMQLLHAIDSLLHSSRTTRSHLVGVRHYSVTPISGRAGLIQWVDNVISVYSVYKCWQARDQAAVLPSSGIGNTKTVAPPVPRPSDMFYGKIIPALKEKGIRRVISRKDWPHEVKRKVLMDLMQEVPRQLLYQELWCASEGFKAFNSKLKRYSGSVAAMSMVGHILGLGDRHLDNILVDFRSGDILHIDYNICFDKGLRLKIPEIVPFRLTQMIEAALGLTGVEGVFKANCEAVLGVLRKNKDILLMLLEVFVWDPLVEWTRGDFHDDAAIGGEERKGMELAVSLSLFASRVQEIRVPLQ 2277          

HSP 2 Score: 1017.68 bits (2630), Expect = 0.000e+0
Identity = 553/917 (60.31%), Postives = 718/917 (78.30%), Query Frame = 3

Query: 7509 LQEHHDLLLATFPAVESALERFRDVLNQYELVSALFYRADQERSNLILHETSAKSIVAEATCNSEKICASFEIQAREFKQAKNVVAEKAQQATTWIEQHGRILDALRGNLIPEISAGMNLSGMADALSLTSAVPEAGVPLTIVPEPTQAQCYDIDREVSQLIAELDRGLSSAVMALQAYSLALQRVLPLNYLTTSTVHGWAQVLQLSANAVSSDILSLARRQASELIAKVHGDNLEFMKSSHDDLCFXXXXXXXXXXXXXXXXXXXXNSIGSKTESKAKDRLMFAFMKYMQSAGLVRKEDANSSLQYGESKYDGTKASRIREDLEEKKDKVLSVLSVAMRSLYDDVKHRILEIYSHTNRAQIENSRPQSDLGTVFSGFEEQVEKCILVAGFVNELWQQIGGDMPGVDRDLYYSKYYSEGNWASIFKTILNCCKSLIGEMTEVVLPDIMRSAVSFNTEVMDAFGLISQIRGSIDTALEQLVEVELERASLAELEQNYFVKVGLITEQQLALEEAAMKGRDHLSWXXXXXXXXXXXXCRAQLEQLHQTWNHRDMRTSSLIKREAEIKSSLVSCEQHFQSLITGDDFRESHRSKSQVLLAILVKPFSELESVDKAXXXXXXXFAPHSDEIPNLVEFLSSGHSVSECVWNFGSLLNNHSFFIWKIGVLDSILDLCIHDMASSVDQNLGFEQLFDVVKRKLEIQLQEYIGRYLKIRIAPALLSWLDKENEHLKLLTEGAKEPSNDHVRKDVEAVKKVQLMLEEYCNTHETARAARSAASVMKRQVNELKEALRKTILEIVQMEWMHDVGLTPSHTSRVLFQKFFSNDDELYPVVLNFSRPKLLETMQSVLSKLARAIEGLKSCEHTSIVAEGQLERAMGWACGGPNSSVAGNSSTKASGIPPEFHDHLMRRRHLLQEAREKA 10259
            LQEHHDLL+++ PAVESALERF DVLN+YE+VSALF RADQ+RSNLILHETSAKSIVAEATCNS+K+ ASFEIQAREF QAK +VAEKAQ+AT+WIEQHGRI+DALRGNLIPEI+A  NLS + +ALSL+SAV  AGVPLT+VPEPTQ QC D+DRE+S+L+AELD G+SS++ A+Q YSLALQR+LPLNYLTTSTV+ WAQ+LQL +  ++SDI+S+ARRQA+ELI+K+  D+ + +K +HDD+C KV KYA EI KVE+ECAEL NS+GS+ ESKAKDRL+ +FMKYMQSA + +K D +S + +G+S+YD T  +R    L EKK+K   VL++A+ S+YDDVK RI+++     +     +R   D  ++    EE VEKC ++  FVN+L Q    D+P  D  +   + + E NWASIFK+ +  CKSL+ ++++ VLPD++RSAV+FN+E  D FG +SQ+RG+ID+ALEQL+EVE+ER ++ ELE+NYFVKV LITEQQLALEEAA+KGRDHLSWEEAEELASQEE+CRAQL++LHQ+WN RD RTSSLIKRE +++S L SC++H+QSL+  ++  +S   + + LL+ LV PF ELES+DK LSS  +S        PNL E +SSG  +SE VW FG  +  ++FFIWK+ VLDS LD+C H++ASS+DQ LGF+QL +  ++KL ++L+++I  YL+ R+ P   +WL+K N+H K L    KE   +   KD+ AVKKVQLMLEEYCN HETARAARSAAS+MKRQV+ELKE ++K  +EIVQMEWMHDV LTPS  SR+   K  ++ D L  ++LN +R +LLE MQS +S++AR IEGL++ E TS+ AEGQLERAMGWACGG +SS+AGNSS+K S IPPEFH+HL RRR LL +AR+++
Sbjct: 2276 LQEHHDLLISSLPAVESALERFSDVLNKYEIVSALFCRADQDRSNLILHETSAKSIVAEATCNSDKLRASFEIQAREFSQAKALVAEKAQEATSWIEQHGRIIDALRGNLIPEINAWNNLSSLGEALSLSSAVLVAGVPLTVVPEPTQVQCTDLDRELSKLVAELDHGVSSSLTAIQVYSLALQRILPLNYLTTSTVYSWAQILQLCSGFLTSDIISIARRQAAELISKLLNDSRDSVKHAHDDMCLKVGKYAFEIGKVEDECAELENSVGSEAESKAKDRLLSSFMKYMQSATVEKKGDLHSLVYHGQSEYDDTNMARYHSQLAEKKEKAFYVLNLAVTSIYDDVKKRIVDMADANGKGN--GNRSLDDSSSLIGELEELVEKCSVITEFVNDLRQFADWDVPCSDTSMESRELFPEENWASIFKSTVVSCKSLVDQLSD-VLPDVIRSAVTFNSEFADVFGSVSQVRGTIDSALEQLLEVEMERGTVVELERNYFVKVALITEQQLALEEAALKGRDHLSWEEAEELASQEESCRAQLDKLHQSWNQRDRRTSSLIKRETDVESVLSSCQRHYQSLMNAEEEIDSLALRGKALLSTLVNPFLELESLDKVLSSDGASSLHAGIRDPNLAERISSGQVLSEKVWKFGGSVKKNAFFIWKVCVLDSFLDVCTHEVASSMDQRLGFDQLVNAARKKLVLRLEKHISHYLRDRMLPIFRAWLEKTNDHSKQLNGSGKELVFEQSHKDLGAVKKVQLMLEEYCNAHETARAARSAASLMKRQVHELKENVKKDCIEIVQMEWMHDVLLTPSPNSRITLHKLLASGDNLQQIILNLNRQRLLEVMQSAVSRIARTIEGLQTVERTSLAAEGQLERAMGWACGGSSSSLAGNSSSKISKIPPEFHEHLTRRRQLLWDARDES 3189          

HSP 3 Score: 532.717 bits (1371), Expect = 2.704e-148
Identity = 310/415 (74.70%), Postives = 354/415 (85.30%), Query Frame = 2

Query:  281 NDSARLAAINSLHRAIIYPPNSILVAHSASFLAQGFSQLLSDKSYAVRQSXXXXXXXXXXVVCSVPIGSSGRQNHVMLSSLVDRFIGWALPLLSNISAGDGTTELALEGLREFLSVGDVGAIERYALPILKACQELLEDERTXXXXXXXXXXXXXXXXXXXXXXFQPHFLDIIDLLLGWALVPDLAESDRRVIMDSFLQFQKHWVGNLQFSLGLLSKFLGDMDVLLQDGTHGTPQQFRRLLALLSCFSTVLQSTASGLLEMNLLEQISEPLSKMLPRLLGCLSVVGKKFGWSRWIEDSWKCLTLLAEILQERFSTFYPLAIDILFQSLELGNTSRPIGAGKITSFQVHGVXXXXXXXXXXXXXXXXPSSVKKILHFDRAISQLRLHPNHLVTGSSAATYV--------FLLQQGN 1501
            ++S RL AI+SLHRAI+YPPNS+LVAHSA+FL+QG  QLLSDKSY+VRQ AA AY ALCAVVCS+PI S+GRQNHVML ++VDRFI W+LPLLSN++AGDGT ELAL+ LREFL+VGDV  IERYALPILKACQ+LLEDERT LSLLHRLL V+TLI LKFS  FQPHF+DI+DLLLGWAL+PD  ESDRRVIMD FLQ QKHWV NLQFSLGL+SKFLGDMD LLQDG+ GTPQQFRRLLALLSCFST+LQSTASGLLE NLL QI EPLSKMLP+LLGCLS++G++FGWS+WI D WKCLTLLAEIL+E FST YPLAI+ LFQS+ + N    +G GK++SFQVHGVLKTNLQLLSLQKLGLL SSV KIL F   +SQLRLHPNH+VT + +  Y+        FLL+  N
Sbjct:   62 DESTRLGAISSLHRAILYPPNSLLVAHSAAFLSQGLLQLLSDKSYSVRQLAATAYGALCAVVCSIPITSNGRQNHVMLGAMVDRFISWSLPLLSNVNAGDGTAELALDSLREFLNVGDVNGIERYALPILKACQQLLEDERTPLSLLHRLLGVITLISLKFSRCFQPHFMDIVDLLLGWALIPDFGESDRRVIMDCFLQCQKHWVSNLQFSLGLMSKFLGDMDALLQDGSLGTPQQFRRLLALLSCFSTILQSTASGLLEANLLGQIREPLSKMLPQLLGCLSLLGQRFGWSKWIGDLWKCLTLLAEILRENFSTLYPLAIETLFQSVRMNNGHSAMGTGKLSSFQVHGVLKTNLQLLSLQKLGLLSSSVHKILQFHAPVSQLRLHPNHVVTANVSTLYLQRSETLACFLLEHLN 476