g.barbadense_cottongen_reftransV1_0094968, g.barbadense_cottongen_reftransV1_0094968 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy Swiss-Prot
Match: SBT4E_ARATH (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 506.523 bits (1303), Expect = 0.000e+0
Identity = 247/394 (62.69%), Postives = 320/394 (81.22%), Query Frame = -2

Query:  110 GIGINLFGANEKFYPIVSGADVSMNAGSKELARFCYGNTLDPAKVKGRLVYCMLGQFDADSXXXXXXXXXXXVESEQYLDTARIFMAPATVVNMTVGETIDKYIHSARSASAVIYGSHEVKVSAPFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTAKPMSKRVNKDAEFSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNCSSLLPGFGYDALNYPSMQLNMKNSQQPTVGVFKRRVTNVGPP-SVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLSASLVWKTTHHTVRSPIVVYS 1288
            G+GI++F    K YP+VSG D + N   K LAR+C+ ++LD  KVKG+++ C +G    +S +K  GG G ++ S+QYLD A+IFMAPAT VN +VG+ I +YI+S RSASAVI  + +V + APF ASFSSRGPNPGS  LLKPDIAAPG+DILAA+TL +SLTGL GDTQ SKFT++SGTSMACPHVAGVAAYVKSFHP WTPAAIKSAI+T+AKP+S+RVNKDAEF+YG GQ+NP RA +PGLVYD+D++SYVQFLC EGY+ +++A L+GT+SV+CSS++PG G+D+LNYP++QL +++++  T+ VF+RRVTNVGPP SVY AT++APKG++ITV P SL F++  QKRSFKVVV AK    T  +++S  LVWK+  H+VRSPIV+YS
Sbjct:  354 GMGISMFSPKAKSYPLVSGVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQM--TPGKIVSGLLVWKSPRHSVRSPIVIYS 745          

HSP 2 Score: 215.698 bits (548), Expect = 0.000e+0
Identity = 119/172 (69.19%), Postives = 148/172 (86.05%), Query Frame = -1

Query: 1359 SKLVGAKYFKLDGNPDPADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVSSGCXXXXXXXXXXXXITDGVDVISISIGGATEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQSFS 1874
            +K++GAKYFK DGN    ++ SPID+DGHGTHTSST+AG LV NASL+G+A GTARGAVPSAR+A+YKVCW  SGCADMDILA  + AI DGV++ISISIGG   D+ +DSISVG+FHA++KGI+TVASAGNDGPS G+++N+ PW+LTVAA+GIDR F+S + LGNG+SFS
Sbjct:  182 NKIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFS 353          

HSP 3 Score: 209.92 bits (533), Expect = 9.342e-56
Identity = 89/157 (56.69%), Postives = 122/157 (77.71%), Query Frame = -1

Query: 2166 KTVSSKEEETKNFYIVYLGDGPLSQASAARKHLSLLSSVKASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQTAKRNLKLEKNIVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNFSGCN 2636
            +  S+++E  K+FYI+YLGD P +     + H++LLSS+  S+ +AKE  +YSYTK+FNAFAAKLS  EA+ +ME++EVV V  N+Y KLHTTKSWDF+G P TAKR+LK E+++++G+LDTGITP S+SF D G GPPP  WKG+C  ++NF+GCN
Sbjct:   25 RYASAEDEHAKDFYIIYLGDRPDNTEETIKTHINLLSSLNISQEEAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRHLKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCN 181          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy Swiss-Prot
Match: CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 287.73 bits (735), Expect = 1.135e-119
Identity = 169/397 (42.57%), Postives = 241/397 (60.71%), Query Frame = -2

Query:  107 QGIGINLFGANEKFYPIVSGADVSMNAGSKELARFCYGNTLDPAKVKGRLVYCMLGQFDADSXXXXXXXXXXXVESEQYLDTARIFMAPATVVNMTVGETIDKYIHSARSASAVIYGSHEV-KVSAPFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTAKPMSKRVNKDAEFSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNCSSLLPGFGYDALNYPSMQLNMKNSQQPTVGVFKRRVTNVGP-PSVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLSASLVWKTTHHTVRSPIVVYSL 1291
            QG+ IN F  + ++YP+VSG D+      K  +RFC   +++P  +KG++V C    F      K + G   V+ +    D A  +  P++V++        +YI+S RS  A I+ S  +   SAP   SFSSRGPN  ++ ++KPDI+ PGV+ILAA+  V  + G++ +T    F ++SGTSM+CPH+ G+A YVK+++PTW+PAAIKSA+MTTA PM+ R N  AEF+YG+G +NP +A+ PGLVYD +E  YV+FLC +GY+  ++  + G  S  C+S   G  +D LNYPS  L++  SQ      F R +T+V P  S Y A I AP+GL I+V P  L F  L  ++SF + V      S K  V+SASLVW    H VRSPI + SL
Sbjct:  347 QGVSINTF--DNQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCE-ASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTL---FNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSA-CTSGNTGRVWD-LNYPSFGLSVSPSQTFNQ-YFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRG----SIKGFVVSASLVWSDGVHYVRSPITITSL 730          

HSP 2 Score: 166.777 bits (421), Expect = 1.135e-119
Identity = 94/172 (54.65%), Postives = 129/172 (75.00%), Query Frame = -1

Query: 1359 KLVGAKYFKLDGNPDPADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVSSGCXXXXXXXXXXXXITDGVDVISISIGGAT-EDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQSFS 1871
            K++GA+ + +     P D+  P D +GHGTHT+ST AG LV  A+L+GL  GTARG VP ARIA YKVCW + GC+D DILAA DDAI DGVD+IS+S+GGA    +  D+I++G+FHA+++GI+T  SAGN GP+  + ++ SPWLL+VAA+ +DR+F + V++GNGQSF 
Sbjct:  177 KIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCW-NDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQ 347          

HSP 3 Score: 156.377 bits (394), Expect = 5.475e-38
Identity = 74/152 (48.68%), Postives = 101/152 (66.45%), Query Frame = -1

Query: 2163 EEETKNFYIVYLGDGPLSQASAARKHLSLLSSVKASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQTAKRNLKLEKNIVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNFSGCNK 2618
            +++ KN YIVY+G       SA   H ++L  V  S   A ES++++Y +SFN FA KL+++EAE +  ++ VV V  N  ++LHTT+SWDF+GFP T  R  ++E NIVVG+LDTGI P+S SF D+GF PPP  WKGTC    NF  CN+
Sbjct:   27 DDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGSTF-APESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFR-CNR 176          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy Swiss-Prot
Match: SBT54_ARATH (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 3.268e-101
Identity = 145/400 (36.25%), Postives = 211/400 (52.75%), Query Frame = -2

Query:  116 EKFYPIVSGADVSMNAGSKELARFCYGNTLDPAKVKGRLVYCMLGQF--------DADSXXXXXXXXXXXVESEQYLDTARIFMAPATVVNMTVGETIDKYIHSARSASAVIYGSHEVKVS--APFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTA-------KPMSKRVNKDAE-FSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNCSSLLPGFGYDALNYPSMQL-NMKNSQQPTVGVFKRRVTNVGPPSVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLSASLVWKTTHHTVRSPIVV 1258
            EK Y ++S AD ++  G+   A  C   +LDP KVKG+++ C+ G           A +   G+          + +  A +   PA+ ++   GET+  Y+ S +     I        +  APF ASFSSRGPN  +  +LKPDI APGV+I+AA+T     T L  D +R+ F   SGTSM+CPH++GV   +K+ HP W+PAAI+SAIMTT+       KPM     K A  FSYG+G + P +A +PGLVYD+    Y+ FLC  GY+ + +          C     G      NYPS+ + N+  S   T     R++ NVGPP+ YNA  + P G++++V P  L F +  + + F++ +  +P   T    +   L W  +HH VRSPIVV
Sbjct:  387 EKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVL--PASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQ---GANLLDFNYPSITVPNLTGSITVT-----RKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTL--RPLPVTPSGYVFGELTWTDSHHYVRSPIVV 774          

HSP 2 Score: 157.918 bits (398), Expect = 3.268e-101
Identity = 90/178 (50.56%), Postives = 122/178 (68.54%), Query Frame = -1

Query: 1362 KLVGAKYFK-----LDGNPDPADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVS---SGCXXXXXXXXXXXXITDGVDVISISIGGATEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQSF 1871
            KL+GA+YF        G P  A   +  D DGHG+HT ST AGN V  A++FG+  GTA G  P AR+A YKVCW     + C D DILAA++ AI DGVDV+S S+GG   D+++D I++G+FHA+K G+  V SAGN GP  G++SN +PW++TV A+ +DR+F++ V+L NGQSF
Sbjct:  198 KLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSF 375          

HSP 3 Score: 104.76 bits (260), Expect = 1.179e-21
Identity = 60/152 (39.47%), Postives = 86/152 (56.58%), Query Frame = -1

Query: 2193 KNFYIVYLGD-GPLSQASAAR------KHLSLLSSVKASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQTA---KRNLKLE----KNIVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCH 2606
            K  YIVYLG    L Q S+A        H + L+S   S  +AKE++ YSY +  N FAA L ++EA  + +  +VV V PN+  KLHTT SW+F+   +     K +L  +    ++ ++  LDTG+ P+S SF D+G+G  P  WKG CH
Sbjct:   39 KKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH 190          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy Swiss-Prot
Match: AIR3_ARATH (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 1.947e-97
Identity = 153/396 (38.64%), Postives = 217/396 (54.80%), Query Frame = -2

Query:  116 KFYPIVSGADVSMNAGSKELARFCYGNTLDPAKVKGRLVYCMLGQFD-ADSXXXXXXXXXXXVESEQYLDTARIFMA-----PATVVNMTVGETIDKYIHSARSASAVIYGSH-EVKVS-APFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTAK-------PMSKRVN-KDAEFSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNCSSLLPGFGYDALNYPSMQLNMKNSQQPTVGVFKRRVTNVGPPSVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLSASLVWKTTHHTVRSPIVV 1255
            KFYPI++  +      S   A+ C   +LDP K KG+++ C+ GQ    +       G G  +  E    T    +A     PAT +       + +YI   +   A I  S  ++ +  AP  ASFSS+GP+  +  +LKPDI APGV ++AAYT   S T  + D +R  F  +SGTSM+CPH++G+A  +K+ +P+W+PAAI+SAIMTTA        P+    N K   FS+GAG + P  A+NPGLVYD+    Y+ FLC  GY+ S I+   G  +  CSS  P      LNYPS+ +    S + TV    R V NVG PS+Y   +  P+G+ + V+PTSL FT++ ++++FKV++V       K  V    LVW    H VRSPIVV
Sbjct:  382 KFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGN-NFTCSS--PKISLVNLNYPSITVPNLTSSKVTV---SRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVF-GELVWSDKKHRVRSPIVV 770          

HSP 2 Score: 147.517 bits (371), Expect = 1.947e-97
Identity = 88/182 (48.35%), Postives = 118/182 (64.84%), Query Frame = -1

Query: 1362 KLVGAKYFK---------LDGNPDPADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVS---SGCXXXXXXXXXXXXITDGVDVISISIGGATEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQSF 1871
            KL+GA+YF          L+ + D     SP D+DGHG+HT ST AG+ V   S+FG   GTA+G  P AR+A YKVCW     + C D D+LAA D AI DG DVIS+S+GG    F  DS+++G+FHA KK I+ V SAGN GP+  ++SN +PW +TV A+ +DR+F S + LGNG+ +
Sbjct:  192 KLIGARYFNKGYAAAVGHLNSSFD-----SPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHY 368          

HSP 3 Score: 112.464 bits (280), Expect = 4.772e-24
Identity = 59/160 (36.88%), Postives = 87/160 (54.37%), Query Frame = -1

Query: 2163 YIVYLGD----GPLSQASAAR---KHLSLLSSVKASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQTAK-------RNLKLEKNIVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNFS-GCNK 2597
            Y+VY G     G +++ +  R    H   L S   S   A +++ YSYTK  N FAA L  D A  + +  EVV V PN+  KLHTT+SWDF+G    +        R  +  ++ ++  LDTG+ P+S SF+D+G GP P  WKG C + ++ +  CN+
Sbjct:   32 YVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNR 191          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 203.371 bits (516), Expect = 5.787e-91
Identity = 146/419 (34.84%), Postives = 212/419 (50.60%), Query Frame = -2

Query:  116 GINLFGAN---EKFYPIVSGADVSMNAGSKELARFCYGNTLDPAKVKGRLVYC--------MLGQFDADSXXXXXXXXXXXVESEQYLDTARIFMAPATVVNMTVGETIDKYIHSAR--SASAVIYGSHEVKVSAPFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTA-------KPMSKRVNK--DAEFSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNCSSLLPGFGYDA--LNYPSMQLNMKNSQQPTVGVFK--RRVTNVGPPSVYNATIKAP-KGLQITVRPTSLLFTRLLQKRSFKV---VVVAKPELSTKFRVLSASLVWKTTHHTVRSPIVV 1282
            G++LF      +K  P +     + NA +      C   TL P KVKG++V C          G     +   G+    T    E+ +  A +   PAT V    G+ I  Y+ +    +AS  I G+      +P  A+FSSRGPN  + ++LKPD+ APGV+ILAA+T     TGL  D++R +F ++SGTSM+CPHV+G+AA +KS HP W+PAAI+SA+MTTA       KP+           F +GAG ++P  A NPGL+YD+    Y+ FLC   Y+   I   +  ++  C    P   Y    LNYPS  +N+       VG +K  R VT+VG    Y+  + +   G++I+V P  L F    +K+S+ V   V  +KP  S  F     S+ W    H V SP+ +
Sbjct:  355 GVSLFKGEALPDKLLPFI----YAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLL--PATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQI-RSVSRRNYTCD---PSKSYSVADLNYPSFAVNVDG-----VGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSF----GSIEWSDGKHVVGSPVAI 754          

HSP 2 Score: 155.606 bits (392), Expect = 5.787e-91
Identity = 91/177 (51.41%), Postives = 120/177 (67.80%), Query Frame = -1

Query: 1359 KLVGAKYFK--LDGNPDPADI----LSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVSSGCXXXXXXXXXXXXITDGVDVISISIGGATEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQSFS 1871
            KL+GA++F    +    P D      SP D DGHGTHTSST AG++V  ASL G A GTARG  P AR+A+YKVCW+  GC   DILAA+D AI D V+V+S+S+GG   D+  D +++GAF A+++GI+   SAGN GPS  SLSN +PW+ TV A  +DR F +   LGNG++F+
Sbjct:  179 KLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLG-GCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFT 354          

HSP 3 Score: 96.2857 bits (238), Expect = 4.465e-19
Identity = 53/155 (34.19%), Postives = 88/155 (56.77%), Query Frame = -1

Query: 2163 YIVYLGDGPLSQASAARKHLSLLSSVKASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIG--------FPQTAKRNLKLEKNIVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNFSG--CNK 2597
            YIV++    +   S+   H +   S   S  D+ E ++Y+Y  + + F+ +L+++EA+ LM    V+ VLP   ++LHTT++  F+G        FP+    +     ++VVG+LDTG+ P+S S+ D+GFGP P +WKG C    NF+   CN+
Sbjct:   32 YIVHMAKSQM--PSSFDLHSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYS-----DVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNR 178          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy Swiss-Prot
Match: SBT16_ARATH (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 9.291e-91
Identity = 155/424 (36.56%), Postives = 219/424 (51.65%), Query Frame = -2

Query:  107 GINLFGA---NEKFYPIVSGADVSMNAGSKELARFCYGNTLDPAKVKGRLVYCMLGQ--------FDADSXXXXXXXXXXXVESEQYLDTARIFMAPATVVNMTVGETIDKYI--HSARSASAVIYGSHEVKVSAPFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTAK--------PMSKRVNKDAE-FSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNC-SSLLPGFGYDALNYPSMQLNMKNSQQPTVG-VFKRRVTNVG-PPSVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLS------ASLVW-KTTHHTVRSPIVVYSL 1282
            G++L+     N + +P+V      M++ S      C  NTLDP +V+G++V C  G             +   G+         E  +  A +   PA  V    G+ I  Y   H    AS    G+      AP  ASFS RGPN  S  +LKPD+ APGV+ILAA+T     TGL  D ++++F ++SGTSMACPHV+G AA +KS HP W+PA I+SA+MTT           + +   K A  + YG+G LN  RA+NPGLVYDI    Y+ FLC  GY   +I  +I    V C ++  P  G   LNYPS+      +++  V     R  TNVG   +VY A I++P+G+ +TV+P  L+FT  +++RS+ V V     ++T+  VL        S+ W     H VRSPIVV  +
Sbjct:  352 GVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLI--PACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTI-QVITRTPVRCPTTRKPSPGN--LNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVT----VNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQM 761          

HSP 2 Score: 142.895 bits (359), Expect = 9.291e-91
Identity = 89/180 (49.44%), Postives = 113/180 (62.78%), Query Frame = -1

Query: 1362 KLVGAKYFK-------LDGNPDPADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVSSGCXXXXXXXXXXXXITDGVDVISISIGGA---TEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQSF 1871
            K++GA++F        + G     + LSP D DGHGTHTSST AG     AS+ G A G A+G  P ARIA YKVCW  SGC D DILAA D A+ DGVDVISISIGG    T  +  D I++G++ A  KGI   +SAGN+GP+  S++N +PW+ TV A+ IDR F +   LG+G   
Sbjct:  171 KIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRL 350          

HSP 3 Score: 79.337 bits (194), Expect = 1.036e-13
Identity = 40/124 (32.26%), Postives = 66/124 (53.23%), Query Frame = -1

Query: 2163 ASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQTAKRNLKLEKN----IVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNFS--GCNK 2516
            ++E   +  +++ Y   F+ F+A ++ DEA+ L     V+ V  +R  +LHTT+S  F+G     ++ L  E +    +++G+ DTGI P+  SF D   GP P  W+G C     FS   CN+
Sbjct:   49 STEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQN--QKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNR 170          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy Swiss-Prot
Match: CRSP_ARATH (CO(2)-response secreted protease OS=Arabidopsis thaliana GN=CRSP PE=2 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 4.630e-87
Identity = 147/429 (34.27%), Postives = 218/429 (50.82%), Query Frame = -2

Query:  110 QGIGINLFGANEK-FYPIVSGADVSMNAGSKELARFCYGNTLDPAKVKGRLVYC--MLGQFDADSXXXXXXXXXXXVESEQYLDTARIFMA-------------PATVVNMTVGETIDKYIHSARSASAVIYGSHEVK--VSAPFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTAKPMSKRVNKD------------AEFSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGT--KSVNCSSLLPGFGYDALNYPSMQLN-MKNSQQPTVGVFKRRVTNVGP--PSVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLSASLVWKTTHHTVRSPIVVYS 1291
            +G GI+    ++   YP++ G        S+  AR C  ++LD  KVKG++V C  + G + A S            E +    T  +F+              P TV++      I  Y++S +   A I  +  V+    AP  A FSSRGP+  ++ +LKPDI APGV ILAA+T   S   L+G    S++ ++SGTSMA PHV+ VA+ +KS HPTW P+AI+SAIMTTA     + N D              +  GAG+L+   ++ PGLVY+  E  Y+ FLC+ GY+ ++I  +     ++  C +         +NYPS+ ++  K +   TV    R VTNVG    +VY  +++ P G  I V P  L FT+  +K +++V+V A   L  K  V  A L W    + VRSPIV+ S
Sbjct:  355 KGEGIHFSNVSKSPVYPLIHGKSAKSADASEGSARACDSDSLDQEKVKGKIVLCENVGGSYYASSARD---------EVKSKGGTGCVFVDDRTRAVASAYGSFPTTVIDSKEAAEIFSYLNSTKDPVATILPTATVEKFTPAPAVAYFSSRGPSSLTRSILKPDITAPGVSILAAWTGNDSSISLEGK-PASQYNVISGTSMAAPHVSAVASLIKSQHPTWGPSAIRSAIMTTA----TQTNNDKGLITTETGATATPYDSGAGELSSTASMQPGLVYETTETDYLNFLCYYGYNVTTIKAMSKAFPENFTCPADSNLDLISTINYPSIGISGFKGNGSKTV---TRTVTNVGEDGEAVYTVSVETPPGFNIQVTPEKLQFTKDGEKLTYQVIVSATASL--KQDVFGA-LTWSNAKYKVRSPIVISS 763          

HSP 2 Score: 151.369 bits (381), Expect = 4.630e-87
Identity = 90/172 (52.33%), Postives = 122/172 (70.93%), Query Frame = -1

Query: 1368 KLVGAKYFKLDGNPDP-ADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVSSGCXXXXXXXXXXXXITDGVDVISISIGG---ATEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQ 1871
            K++GA+Y+K   NPD  ++  +  DV GHG+H SST+AG+ V NAS +G+A GTA+G   +ARIA+YKVC    GC    ILAA DDAI DGVDV+S+S+G    A  D  TD I++GAFHA+++GI+ + SAGNDGP  G+++N +PW++TVAA  IDR F S V LG  +
Sbjct:  185 KIIGARYYK---NPDDDSEYYTTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGGSQNARIAMYKVC-NPGGCTGSSILAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHAVEQGILVICSAGNDGPDGGTVTNTAPWIMTVAANTIDRDFESDVVLGGNK 352          

HSP 3 Score: 97.4413 bits (241), Expect = 2.465e-19
Identity = 53/154 (34.42%), Postives = 87/154 (56.49%), Query Frame = -1

Query: 2163 YIVYLGDGPLSQASAARKHLSLLSSVKASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQTAKRNLKLEKNI-------VVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNF--SGCNK 2597
            YIVY+G    +  +   + L + +  K   +D    ++++Y   F+ FAA+L+ +EA+++ +   VV V P+ + +LHTT SWDF+ +  + K +     +        +VG+LDTGI P+S+SF D   GP P  WKGTC   ++F  S CN+
Sbjct:   36 YIVYMGSASSAANANRAQIL-INTMFKRRAND----LLHTYKHGFSGFAARLTAEEAKVIAKKPGVVSVFPDPHFQLHTTHSWDFLKYQTSVKVDSGPPSSASDGSYDSIVGILDTGIWPESESFNDKDMGPIPSRWKGTCMEAKDFKSSNCNR 184          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy Swiss-Prot
Match: SBT12_ARATH (Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana GN=SBT1.2 PE=2 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 4.953e-62
Identity = 146/390 (37.44%), Postives = 226/390 (57.95%), Query Frame = -2

Query:  116 GSKELARFCYGNTLDPAKVKGRLVYCMLG------QFDADSXXXXXXXXXXXVESEQYLDTARIFMAPATVVNMTVGETIDKYIH-SARSASAVIYGSHEVKVS-APFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTAKPMSK--RVNKDAE-----FSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNCSSLL---PGFGYDALNYPSMQLNMKNSQQPTVGVFKRRVTNVGPP-SVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLS---ASLVWKTTHH---TVRSPIVV 1210
            G  + + FC   +L   +++G++V C  G      + +A     G+  I    E  Q  D+  + + PAT++  T    +  Y++ + +  + +I+G   +  S AP  A FS+RGP+  +  +LKPD+ APGV+I+AA+      TGL  D++R  FT+MSGTSM+CPHV+G+ A ++S +P W+PAAIKSA+MTTA    +  +  KD       F+ GAG +NP +AINPGLVY+I  + Y+ +LC  G++ S I   I  K+V+C+ +L   PGF   +LNYPS+ +  K  +  T  +  RRVTNVG P S+Y+  +KAP+G+++ V P  L+F  + Q  S++V  V K + +   +V S     L W  +H+    VRSPI V
Sbjct:  388 GGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEAVKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFTVMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDIL-AITHKNVSCNGILRKNPGF---SLNYPSIAVIFKRGK--TTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKK-NRGGKVASFAQGQLTWVNSHNLMQRVRSPISV 770          

HSP 2 Score: 134.42 bits (337), Expect = 5.607e-31
Identity = 79/182 (43.41%), Postives = 118/182 (64.84%), Query Frame = -1

Query: 1368 SSIYSKLVGAKYF----KLDGNPD-----PADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVSSGCXXXXXXXXXXXXITDGVDVISISIGGATEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQ 1886
            SS   KL+GA++F    ++  +P+     P + +S  D  GHGTHT+ST+ G+ V  A++ G   G ARG  P A IA+YKVCW + GC   DILAA+D AI D VDV+S+S+GG       D+I++G F A+++GI  + +AGN+GP   S++N +PW+ T+ A  +DR+F + V+L NG+
Sbjct:  181 SSCNRKLIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKVCWFN-GCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGK 361          

HSP 3 Score: 88.5817 bits (218), Expect = 1.295e-16
Identity = 57/168 (33.93%), Postives = 84/168 (50.00%), Query Frame = -1

Query: 2163 SSKEEETKNFYIVYLGDGPLSQASAARK---HLSLLSS----VKASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQTAKRNL----KLEKNIVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNF--SGCNK 2627
            SS E   K  YIV L     +  + A K   HLS L      V+  E +    ++YSY  +   FAA+L++ EAE+L    EVV V P+   ++ TT S+ F+G        +    +  +  ++G+LDTG+ P+S SF D G    P  WKG C   ++F  S CN+
Sbjct:   18 SSSEILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNR 185          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy Swiss-Prot
Match: SBT1_SOYBN (Subtilisin-like protease Glyma18g48580 OS=Glycine max GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 213.386 bits (542), Expect = 1.030e-56
Identity = 123/279 (44.09%), Postives = 166/279 (59.50%), Query Frame = -2

Query:  116 APFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRS-KFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTA-------KPMSKRVNK---DAEFSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNCSSLLPGFGYDALNYPSMQLNMKNSQQPTVGVFKRRVTNVGPPSVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLSASLVWKTTHHTVRSPIVV 919
            AP  ASFSSRGPN     +LKPD+ APGV+ILAAY+   S + L  D +R  KF ++ GTSM+CPH +G+A  +K+ HP+W+PAAIKSAIMTTA       +P+    +K   DA F+YG+G + P  AI PGLVYD+    Y+ FLC  GY    I+ L   ++  CS        + LNYPS+ L     +  T+    R VTNVGPPS Y  + ++P G  I V P SL FT++ ++++FKV+V A    +T+ +     L W    H VRSPI V
Sbjct:  516 APVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADA-FAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSG---SHSVNDLNYPSITLPNLRLKPVTIA---RTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQAS-SAATRRKYEFGDLRWTDGKHIVRSPITV 786          

HSP 2 Score: 137.117 bits (344), Expect = 8.429e-32
Identity = 84/178 (47.19%), Postives = 114/178 (64.04%), Query Frame = -1

Query: 1374 KLVGAKYFKL-----DGNPDPADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWV---SSGCXXXXXXXXXXXXITDGVDVISISIGGA----TEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGN 1871
            KL+GA+Y+       +G  DP  + +  D  GHGTHT ST  GN V  A +F +  GTA+G  P AR+A YKVCW     + C   D+LAA+D AI DGVDVI++S G +     E   TD IS+GAFHA+ K I+ VASAGNDGP+ G+++N +PW+ T+AA+ +DR F S + + N
Sbjct:  193 KLIGARYYNKAFEAHNGQLDPL-LHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTINN 369          

HSP 3 Score: 97.4413 bits (241), Expect = 2.367e-19
Identity = 60/165 (36.36%), Postives = 90/165 (54.55%), Query Frame = -1

Query: 2163 TKNFYIVYLG---DGPLSQAS----AARKHLSLLSSVKASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQ----TAKRNLKLEKNIVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNFSG-----CNK 2609
            +K  YIVY+G    GP   ++    A   H  LL S+  S   AKE++IYSY +  N FAA L ++EA  + +   VV V  ++ HKLHTT+SW+F+G  +    +A +  +  +N ++G +DTG+ P+S SF D G+G  P  W+G         G     CN+
Sbjct:   28 SKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRGQNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNR 192          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy Swiss-Prot
Match: SBT11_ARATH (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 205.297 bits (521), Expect = 3.716e-54
Identity = 138/391 (35.29%), Postives = 208/391 (53.20%), Query Frame = -2

Query:  116 AGSKELARFCYGNTLDPAKVKGRLVYCMLG--------QFDADSXXXXXXXXXXXVESEQYLDTARIFMAPATVVNMTVGETIDKYIHSARSASA-VIYGSHEVKVSAPFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTA-------KPMSKR-----VNKDAEFSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTK---SVNCSSLLPGFGYDALNYPSMQLNMKNSQQPTVGVFKRRVTNVGPPSV-YNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLSASLVWKTTHHTVRSPIVV 1213
            AG +  A FC  ++L    V+G++V C+ G        +    S    +  + T  E E+ L    +   PA  +  + G+T+  Y+  A +A+A V +       +AP  A+FSSRGP+     + KPDIAAPG++ILA ++   S + L+ D +R +F ++SGTSMACPH++G+AA +KS H  W+PA IKSAIMTTA       +P+  R      +    F++GAG ++P RA++PGLVYD   + Y+ +LC   Y+   I    GT    + N   L PG     LNYPS  +N+ N        +KR VTNVG P+  Y   ++ PKG+++ V P  L F +  ++ S+ V   A+   ++        LVW    + VRSPI V
Sbjct:  388 AGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVL--PAVSLGFSDGKTLLNYLAGAANATASVRFRGTAYGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPG----DLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTVTYDAEASRNSSSSSFGV-LVWICDKYNVRSPIAV 771          

HSP 2 Score: 135.961 bits (341), Expect = 1.896e-31
Identity = 77/156 (49.36%), Postives = 106/156 (67.95%), Query Frame = -1

Query: 1362 DPADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVSSGCXXXXXXXXXXXXITDGVDVISISIGGATEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQSF 1829
            +  D  S  D  GHGTHT+ST AG++V  A+ FG AKG A G   ++RIA YK CW + GCA  D++AA+D AI DGVDVIS+S+GG++  F  D I++  F A++K I    SAGN GP+  ++SN +PWL+TVAA+  DR F + V++GN +S 
Sbjct:  212 ETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTSRIAAYKACW-ALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSL 366          

HSP 3 Score: 72.4034 bits (176), Expect = 1.289e-11
Identity = 36/111 (32.43%), Postives = 58/111 (52.25%), Query Frame = -1

Query: 2163 YSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIG--FPQTAKRNLKLEKNIVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNFSG--CNK 2483
            Y Y  + + F+A L+ D+ + +      +   P+    LHTT S +F+G  F         L  ++++G++DTGI+P+  SF+D    P P  W+G+C    NFS   CNK
Sbjct:   81 YIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNK 191          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy TrEMBL
Match: A0A072TND2_MEDTR (Subtilisin-like serine protease OS=Medicago truncatula GN=MTR_8g033150 PE=4 SV=1)

HSP 1 Score: 515.383 bits (1326), Expect = 0.000e+0
Identity = 251/394 (63.71%), Postives = 329/394 (83.50%), Query Frame = -2

Query:  110 GIGINLFGANEKFYPIVSGADVSMNAGSKELARFCYGNTLDPAKVKGRLVYCMLGQFDADSXXXXXXXXXXXVESEQYLDTARIFMAPATVVNMTVGETIDKYIHSARSASAVIYGSHEVKVSAPFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTAKPMSKRVNKDAEFSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNCSSLLPGFGYDALNYPSMQLNMKNSQQPTVGVFKRRVTNVGP-PSVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLSASLVWKTTHHTVRSPIVVYS 1288
            G G++ F   +K YP+V+G D +  + SKE A+FC G++L+P KVKG++VYC    +  D+VVK IGGIGT++E++Q++D A+IF APAT VN + G+ I  YI S RS SAVI+ S EVK+ APF ASFSSRGPNPGSQ +LKPDI APG++ILAAYTL  S++GL+GDTQ S+FTLMSGTSM+CPHV+GVAAYVKSFHP WTPAAI+SAI+TTAKPMS++VN++AEF++GAGQ+NP RA+NPGLVYD+D+ +Y+QFLCHEGY+GS+++ LIG+ S+NC+SLLPG G+DA+NYPSMQLN+K +   T+GVF+RRVTNVGP  +++NATIK+PKG++ITV+PTSL+F+  LQKRSFKVVV AK   S K  ++SASL+W++  + VRSPIV+YS
Sbjct:  336 GEGVSTFSPKQKQYPLVNGMDAARASSSKEDAKFCDGDSLEPKKVKGKIVYCRYRTWGTDAVVKAIGGIGTIIENDQFVDFAQIFSAPATFVNESTGQAITNYIKSTRSPSAVIHKSQEVKIPAPFVASFSSRGPNPGSQRILKPDITAPGINILAAYTLKTSISGLEGDTQFSEFTLMSGTSMSCPHVSGVAAYVKSFHPDWTPAAIRSAIITTAKPMSQKVNREAEFAFGAGQVNPTRAVNPGLVYDMDDFAYIQFLCHEGYNGSTLSVLIGS-SINCTSLLPGIGHDAINYPSMQLNVKRNTDTTIGVFRRRVTNVGPGQTIFNATIKSPKGVEITVKPTSLIFSHTLQKRSFKVVVKAKSMASMK--IVSASLIWRSPRYIVRSPIVIYS 726          

HSP 2 Score: 230.335 bits (586), Expect = 0.000e+0
Identity = 116/172 (67.44%), Postives = 149/172 (86.63%), Query Frame = -1

Query: 1359 SKLVGAKYFKLDGNPDPADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVSSGCXXXXXXXXXXXXITDGVDVISISIGGATEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQSFS 1874
            +K++GAKYFKLDG  +P+DILSPIDV+GHGTHT+ST AGN+V NASLFGLAKG ARGAV SAR+AIYK+CW   GCADMDILAA + AI DGVDVIS+S+GG  E++  DSI++GAFHA++KGIITVASAGN GP++ ++ NN+PW++TVAA+GIDR F+S ++LG+ ++ S
Sbjct:  164 NKIIGAKYFKLDGRSNPSDILSPIDVEGHGTHTASTAAGNIVPNASLFGLAKGMARGAVHSARLAIYKICWTEDGCADMDILAAFEAAIHDGVDVISVSLGGGNENYAQDSIAIGAFHAMRKGIITVASAGNGGPTMATVVNNAPWIVTVAASGIDRDFQSTIELGSRKNVS 335          

HSP 3 Score: 215.312 bits (547), Expect = 1.122e-55
Identity = 99/158 (62.66%), Postives = 121/158 (76.58%), Query Frame = -1

Query: 2166 KTVSSKEEETKNFYIVYLG-DGPLSQASAARKHLSLLSSVKASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQTAKRNLKLEKNIVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNFSGCN 2636
            KT +     T NFYIV+LG D P+S+  A   HL++LS+VK S  +AKES++YSYTKSFNAFAAKLS+DEA  L  ++EV+ V+PN+Y KLHTT+SWDFIG P TAKR LK E + +V +LDTGITP+  SFKDDGFGPPP  WKGTC  + NFSGCN
Sbjct:    6 KTATPTTIRTMNFYIVFLGGDHPVSREGAVETHLNILSAVKESHVEAKESIVYSYTKSFNAFAAKLSEDEANKLSSMNEVLSVIPNQYRKLHTTRSWDFIGLPLTAKRKLKSEGDTIVALLDTGITPEFQSFKDDGFGPPPAKWKGTCDKYVNFSGCN 163          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy TrEMBL
Match: A0A072TZN1_MEDTR (Subtilisin-like serine protease OS=Medicago truncatula GN=MTR_8g033150 PE=4 SV=1)

HSP 1 Score: 515.383 bits (1326), Expect = 0.000e+0
Identity = 251/394 (63.71%), Postives = 329/394 (83.50%), Query Frame = -2

Query:  110 GIGINLFGANEKFYPIVSGADVSMNAGSKELARFCYGNTLDPAKVKGRLVYCMLGQFDADSXXXXXXXXXXXVESEQYLDTARIFMAPATVVNMTVGETIDKYIHSARSASAVIYGSHEVKVSAPFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTAKPMSKRVNKDAEFSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNCSSLLPGFGYDALNYPSMQLNMKNSQQPTVGVFKRRVTNVGP-PSVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLSASLVWKTTHHTVRSPIVVYS 1288
            G G++ F   +K YP+V+G D +  + SKE A+FC G++L+P KVKG++VYC    +  D+VVK IGGIGT++E++Q++D A+IF APAT VN + G+ I  YI S RS SAVI+ S EVK+ APF ASFSSRGPNPGSQ +LKPDI APG++ILAAYTL  S++GL+GDTQ S+FTLMSGTSM+CPHV+GVAAYVKSFHP WTPAAI+SAI+TTAKPMS++VN++AEF++GAGQ+NP RA+NPGLVYD+D+ +Y+QFLCHEGY+GS+++ LIG+ S+NC+SLLPG G+DA+NYPSMQLN+K +   T+GVF+RRVTNVGP  +++NATIK+PKG++ITV+PTSL+F+  LQKRSFKVVV AK   S K  ++SASL+W++  + VRSPIV+YS
Sbjct:  363 GEGVSTFSPKQKQYPLVNGMDAARASSSKEDAKFCDGDSLEPKKVKGKIVYCRYRTWGTDAVVKAIGGIGTIIENDQFVDFAQIFSAPATFVNESTGQAITNYIKSTRSPSAVIHKSQEVKIPAPFVASFSSRGPNPGSQRILKPDITAPGINILAAYTLKTSISGLEGDTQFSEFTLMSGTSMSCPHVSGVAAYVKSFHPDWTPAAIRSAIITTAKPMSQKVNREAEFAFGAGQVNPTRAVNPGLVYDMDDFAYIQFLCHEGYNGSTLSVLIGS-SINCTSLLPGIGHDAINYPSMQLNVKRNTDTTIGVFRRRVTNVGPGQTIFNATIKSPKGVEITVKPTSLIFSHTLQKRSFKVVVKAKSMASMK--IVSASLIWRSPRYIVRSPIVIYS 753          

HSP 2 Score: 230.335 bits (586), Expect = 0.000e+0
Identity = 116/172 (67.44%), Postives = 149/172 (86.63%), Query Frame = -1

Query: 1359 SKLVGAKYFKLDGNPDPADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVSSGCXXXXXXXXXXXXITDGVDVISISIGGATEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQSFS 1874
            +K++GAKYFKLDG  +P+DILSPIDV+GHGTHT+ST AGN+V NASLFGLAKG ARGAV SAR+AIYK+CW   GCADMDILAA + AI DGVDVIS+S+GG  E++  DSI++GAFHA++KGIITVASAGN GP++ ++ NN+PW++TVAA+GIDR F+S ++LG+ ++ S
Sbjct:  191 NKIIGAKYFKLDGRSNPSDILSPIDVEGHGTHTASTAAGNIVPNASLFGLAKGMARGAVHSARLAIYKICWTEDGCADMDILAAFEAAIHDGVDVISVSLGGGNENYAQDSIAIGAFHAMRKGIITVASAGNGGPTMATVVNNAPWIVTVAASGIDRDFQSTIELGSRKNVS 362          

HSP 3 Score: 216.083 bits (549), Expect = 9.101e-56
Identity = 98/150 (65.33%), Postives = 119/150 (79.33%), Query Frame = -1

Query: 2166 ETKNFYIVYLG-DGPLSQASAARKHLSLLSSVKASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQTAKRNLKLEKNIVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNFSGCN 2612
            E KNFYIV+LG D P+S+  A   HL++LS+VK S  +AKES++YSYTKSFNAFAAKLS+DEA  L  ++EV+ V+PN+Y KLHTT+SWDFIG P TAKR LK E + +V +LDTGITP+  SFKDDGFGPPP  WKGTC  + NFSGCN
Sbjct:   41 EQKNFYIVFLGGDHPVSREGAVETHLNILSAVKESHVEAKESIVYSYTKSFNAFAAKLSEDEANKLSSMNEVLSVIPNQYRKLHTTRSWDFIGLPLTAKRKLKSEGDTIVALLDTGITPEFQSFKDDGFGPPPAKWKGTCDKYVNFSGCN 190          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy TrEMBL
Match: W9S5Q3_9ROSA (Xylem serine proteinase 1 OS=Morus notabilis GN=L484_020785 PE=4 SV=1)

HSP 1 Score: 514.998 bits (1325), Expect = 0.000e+0
Identity = 256/380 (67.37%), Postives = 317/380 (83.42%), Query Frame = -2

Query:  152 GIGINLFGANEKFYPIVSGADVSMNAGSKELARFCYGNTLDPAKVKGRLVYCMLGQFDADSXXXXXXXXXXXVESEQYLDTARIFMAPATVVNMTVGETIDKYIHSARSASAVIYGSHEVKVSAPFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTAKPMSKRVNKDAEFSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNCSSLLPGFGYDALNYPSMQLNMKNSQQPTVGVFKRRVTNVGPP-SVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLSASLVWK 1288
            G GI+ F   +K YP+VSG D++ N+ SKE ARFCY ++LD  KVKG+LV C L  + ADSV+KGIGGIGT+VES+Q+LD A+IFMAPAT+VN T+GETI+ YIHS  SA+AVI+ S+EV+V APF ASFSSRGP+PG++ +LKPDIAAPG+DILA+YT +KSLTGLKGDTQ SKFT+MSGTSMACPHVAGVAAYVKSFHP W+PAAI+SAI+TTA PMS RVN +AEF+YGAGQLNP RA+NPGL+YD++ MSY+QFLC EGY  SS+A LIG+K VNCSSL+PG GYD LNYP+ QL +K  Q+ T+ +++R VTNVGPP S YNAT+ APKG++ITV P SL+FT+ +QKRSFKVVV AK + S + +++S  L WK
Sbjct:  353 GSGISTFHPTQKMYPLVSGDDIAHNSASKEDARFCYADSLDSKKVKGKLVLCKLASWGADSVIKGIGGIGTIVESDQFLDAAQIFMAPATIVNSTIGETINHYIHSTGSATAVIHKSNEVQVRAPFIASFSSRGPSPGTKRILKPDIAAPGIDILASYTPLKSLTGLKGDTQFSKFTIMSGTSMACPHVAGVAAYVKSFHPHWSPAAIRSAILTTATPMSPRVNAEAEFAYGAGQLNPRRALNPGLIYDMNNMSYIQFLCLEGYKDSSLAPLIGSKFVNCSSLIPGLGYDGLNYPTFQLTLKRKQKTTLAIYRRTVTNVGPPRSSYNATVIAPKGVEITVTPMSLVFTKAMQKRSFKVVVKAKWDPSREGQMVSGRLAWK 732          

HSP 2 Score: 248.825 bits (634), Expect = 0.000e+0
Identity = 125/170 (73.53%), Postives = 154/170 (90.59%), Query Frame = -1

Query: 1365 SKLVGAKYFKLDGNPDPADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVSSGCXXXXXXXXXXXXITDGVDVISISIGGATEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQS 1874
            +K++GA+YFKLDGNPDP+D+LSP+D DGHGTHTSSTLAG+ V NASLFGLAKGT+RGAVPSAR+A+YKVCW+SSGC+D+DILAA D AI DGVDVIS+SIGG + D+  DSI++G+FHA+  GIITV SAGNDGPSLGS+SN++PWLLTVAA+GIDR F+S V+ G+G+S
Sbjct:  181 NKIIGARYFKLDGNPDPSDVLSPLDADGHGTHTSSTLAGDNVPNASLFGLAKGTSRGAVPSARVAMYKVCWISSGCSDIDILAAFDAAIHDGVDVISLSIGGGSADYTADSIAIGSFHAMNNGIITVTSAGNDGPSLGSVSNHAPWLLTVAASGIDRAFKSQVQFGDGKS 350          

HSP 3 Score: 220.32 bits (560), Expect = 3.808e-57
Identity = 106/159 (66.67%), Postives = 122/159 (76.73%), Query Frame = -1

Query: 2166 KTVSSKEEETKNFYIVYLGDGPLSQASAARKHLSLLSSVKASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQTAKRNLKLEKNIVVGMLDTGITPQSDSFKDDGFGP--PPHNWKGTCHHFQNFSGCN 2636
            + VSSK  E K FYI+Y G  P+ + SA RKH+ +LSSVK S  +AKES++YSYTKSFNAFAA+LS DEA  + E+DEV+ V+PNRY KLHTTKSWDFIG P TA+R LK E NIVVG+ DTGITPQSDSFKD G  P  PPH WKGTC    NFSGCN
Sbjct:   23 QLVSSKGSEEKKFYIIYFGGRPVEEDSAVRKHIDVLSSVKESFVEAKESIVYSYTKSFNAFAAELSSDEANKIAEMDEVLSVIPNRYRKLHTTKSWDFIGLPLTARRKLKAESNIVVGLFDTGITPQSDSFKDYGLTPDLPPH-WKGTCDVSANFSGCN 180          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy TrEMBL
Match: A0A0B2RC14_GLYSO (Xylem serine proteinase 1 OS=Glycine soja GN=glysoja_005141 PE=4 SV=1)

HSP 1 Score: 514.612 bits (1324), Expect = 0.000e+0
Identity = 256/392 (65.31%), Postives = 324/392 (82.65%), Query Frame = -2

Query:  116 GIGINLFGANEKFYPIVSGADVSMNAGSKELARFCYGNTLDPAKVKGRLVYCMLGQFDADSXXXXXXXXXXXVESEQYLDTARIFMAPATVVNMTVGETIDKYIHSARSASAVIYGSHEVKVSAPFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTAKPMSKRVNKDAEFSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNCSSLLPGFGYDALNYPSMQLNMKNSQQPTVGVFKRRVTNVGP-PSVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLSASLVWKTTHHTVRSPIVV 1288
            G+G+N F    K YP+++G D + ++  KE A FCY  TL P KVKG+LVYC LG +  +SVVKGIGGIGT++ES+QY D A+IFMAPAT+V    G+TI KYI S RS SAVIY S E+++ APF ASFSSRGPNPGSQ++LKPD+AAPG+DILA+YTL KSLTGLKGDTQ S+F LMSGTSMACPHVAGVA+YVKSFHP WTPAAI+SAI+TTAKPMSKRVN +AEF+YGAGQLNP  A++PGLVYD+D + Y+QFLCHEGY GSS++ L+G+  VNCSSLLPG G+DA+NYP+MQL++++++   VGVF+R VTNVGP P++YNAT+++PKG++ITV+PTSL F++ +QKRSFKVVV A    S K  ++S SL+W++  + VRSPIV+
Sbjct:  281 GVGVNCFDPKGKQYPLINGVDAAKDSKDKEDAGFCYEGTLQPNKVKGKLVYCKLGTWGTESVVKGIGGIGTLIESDQYPDVAQIFMAPATIVTSGTGDTITKYIQSTRSPSAVIYKSREMQMQAPFTASFSSRGPNPGSQNVLKPDVAAPGLDILASYTLRKSLTGLKGDTQFSEFILMSGTSMACPHVAGVASYVKSFHPHWTPAAIRSAIITTAKPMSKRVNNEAEFAYGAGQLNPRSAVSPGLVYDMDALGYIQFLCHEGYKGSSLSALVGSP-VNCSSLLPGLGHDAINYPTMQLSLESNKGTRVGVFRRTVTNVGPAPTIYNATVRSPKGVEITVKPTSLTFSKTMQKRSFKVVVKATSIGSEK--IVSGSLIWRSPRYIVRSPIVI 669          

HSP 2 Score: 251.521 bits (641), Expect = 0.000e+0
Identity = 131/171 (76.61%), Postives = 153/171 (89.47%), Query Frame = -1

Query: 1359 KLVGAKYFKLDGNPDPADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVSSGCXXXXXXXXXXXXITDGVDVISISIGGATEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQSFS 1871
            K++GAKYFK DGNPDP+DILSP+D DGHGTHT+ST+AGNLV NA+LFGLA GTARGAVPSAR+AIYKVCW SSGCADMDILAA D AI DGVDVISISIGG    +V DSIS+GAFHA++KGIITVASAGN GPSLG+++N +PW++TVAA+GIDR FRS V+LGNG++ S
Sbjct:  110 KIIGAKYFKADGNPDPSDILSPVDADGHGTHTASTVAGNLVPNANLFGLANGTARGAVPSARLAIYKVCWSSSGCADMDILAAFDAAIHDGVDVISISIGGGNPSYVEDSISIGAFHAMRKGIITVASAGNSGPSLGTVTNTAPWIVTVAASGIDRTFRSTVQLGNGKNVS 280          

HSP 3 Score: 173.326 bits (438), Expect = 6.728e-42
Identity = 73/109 (66.97%), Postives = 89/109 (81.65%), Query Frame = -1

Query: 2163 MIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQTAKRNLKLEKNIVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNFSGCNK 2489
            M+YSYTK+ NAFAAKLS+DEA+ L  +DEV+ V  N+Y +LHTT+SW+FIG P TAKR LK E +I+V +LDTG TP+S SFKDDGFGPPP  WKG+C H+ NFSGCNK
Sbjct:    1 MVYSYTKTLNAFAAKLSEDEAKKLSAMDEVLLVFQNQYRQLHTTRSWNFIGLPTTAKRRLKSESDIIVALLDTGFTPESKSFKDDGFGPPPARWKGSCGHYANFSGCNK 109          

BLAST of g.barbadense_cottongen_reftransV1_0094968 vs. ExPASy TrEMBL
Match: I1L6R8_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=2)

HSP 1 Score: 513.842 bits (1322), Expect = 0.000e+0
Identity = 256/392 (65.31%), Postives = 324/392 (82.65%), Query Frame = -2

Query:  116 GIGINLFGANEKFYPIVSGADVSMNAGSKELARFCYGNTLDPAKVKGRLVYCMLGQFDADSXXXXXXXXXXXVESEQYLDTARIFMAPATVVNMTVGETIDKYIHSARSASAVIYGSHEVKVSAPFAASFSSRGPNPGSQHLLKPDIAAPGVDILAAYTLVKSLTGLKGDTQRSKFTLMSGTSMACPHVAGVAAYVKSFHPTWTPAAIKSAIMTTAKPMSKRVNKDAEFSYGAGQLNPARAINPGLVYDIDEMSYVQFLCHEGYSGSSIAHLIGTKSVNCSSLLPGFGYDALNYPSMQLNMKNSQQPTVGVFKRRVTNVGP-PSVYNATIKAPKGLQITVRPTSLLFTRLLQKRSFKVVVVAKPELSTKFRVLSASLVWKTTHHTVRSPIVV 1288
            G+G+N F    K YP+++G D + ++  KE A FCY  TL P KVKG+LVYC LG +  +SVVKGIGGIGT++ES+QY D A+IFMAPAT+V    G+TI KYI S RS SAVIY S E+++ APF ASFSSRGPNPGSQ++LKPD+AAPG+DILA+YTL KSLTGLKGDTQ S+F LMSGTSMACPHVAGVA+YVKSFHP WTPAAI+SAI+TTAKPMSKRVN +AEF+YGAGQLNP  A++PGLVYD+D + Y+QFLCHEGY GSS++ L+G+  VNCSSLLPG G+DA+NYP+MQL++++++   VGVF+R VTNVGP P++YNAT+++PKG++ITV+PTSL F++ +QKRSFKVVV A    S K  ++S SL+W++  + VRSPIV+
Sbjct:  320 GVGVNCFDPKGKQYPLINGVDAAKDSKDKEDAGFCYEGTLQPNKVKGKLVYCKLGTWGTESVVKGIGGIGTLIESDQYPDVAQIFMAPATIVTSGTGDTITKYIQSTRSPSAVIYKSREMQMQAPFTASFSSRGPNPGSQNVLKPDVAAPGLDILASYTLRKSLTGLKGDTQFSEFILMSGTSMACPHVAGVASYVKSFHPHWTPAAIRSAIITTAKPMSKRVNNEAEFAYGAGQLNPRSAVSPGLVYDMDALGYIQFLCHEGYKGSSLSALVGSP-VNCSSLLPGLGHDAINYPTMQLSLESNKGTRVGVFRRTVTNVGPAPTIYNATVRSPKGVEITVKPTSLTFSKTMQKRSFKVVVKATSIGSEK--IVSGSLIWRSPRYIVRSPIVI 708          

HSP 2 Score: 248.825 bits (634), Expect = 0.000e+0
Identity = 130/171 (76.02%), Postives = 152/171 (88.89%), Query Frame = -1

Query: 1359 KLVGAKYFKLDGNPDPADILSPIDVDGHGTHTSSTLAGNLVHNASLFGLAKGTARGAVPSARIAIYKVCWVSSGCXXXXXXXXXXXXITDGVDVISISIGGATEDFVTDSISVGAFHALKKGIITVASAGNDGPSLGSLSNNSPWLLTVAATGIDRQFRSAVKLGNGQSFS 1871
            K++GAKYFK DGNPDP+DILSP+D DGHGTHT+ST+AGNLV NA+LFGLA GTARGAVPSAR+AIYKVCW SSGCADMDILAA D AI DGVDVISISIGG    +V  SIS+GAFHA++KGIITVASAGN GPSLG+++N +PW++TVAA+GIDR FRS V+LGNG++ S
Sbjct:  149 KIIGAKYFKADGNPDPSDILSPVDADGHGTHTASTVAGNLVPNANLFGLANGTARGAVPSARLAIYKVCWSSSGCADMDILAAFDAAIHDGVDVISISIGGGNPSYVEGSISIGAFHAMRKGIITVASAGNSGPSLGTVTNTAPWIVTVAASGIDRTFRSTVQLGNGKNVS 319          

HSP 3 Score: 205.682 bits (522), Expect = 1.517e-52
Identity = 92/147 (62.59%), Postives = 116/147 (78.91%), Query Frame = -1

Query: 2163 NFYIVYLGDGPLSQASAARKHLSLLSSVKASEHDAKESMIYSYTKSFNAFAAKLSKDEAEMLMEVDEVVCVLPNRYHKLHTTKSWDFIGFPQTAKRNLKLEKNIVVGMLDTGITPQSDSFKDDGFGPPPHNWKGTCHHFQNFSGCNK 2603
            NFYIV+LG    S+ +A   +L++LS+VK S  +AKESM+YSYTK+ NAFAAKLS+DEA+ L  +DEV+ V  N+Y +LHTT+SW+FIG P TAKR LK E +I+V +LDTG TP+S SFKDDGFGPPP  WKG+C H+ NFSGCNK
Sbjct:    2 NFYIVFLGAHTESRGNALDTYLNVLSAVKESFLEAKESMVYSYTKTLNAFAAKLSEDEAKKLSAMDEVLLVFQNQYRQLHTTRSWNFIGLPTTAKRRLKSESDIIVALLDTGFTPESKSFKDDGFGPPPARWKGSCGHYANFSGCNK 148