g.barbadense_cottongen_reftransV1_0094223, g.barbadense_cottongen_reftransV1_0094223 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: LPE1_MAIZE (Nucleobase-ascorbate transporter LPE1 OS=Zea mays GN=LPE1 PE=1 SV=2)

HSP 1 Score: 125.946 bits (315), Expect = 1.496e-28
Identity = 110/329 (33.43%), Postives = 146/329 (44.38%), Query Frame = 1

Query: 2401 LPQFILLNNFGLSRKMVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQ 3387
            LP  +LL  F    +    LF  G F   R  ++      ++V I WIYA  LTA GAY+ +G                    T   CRAD S   + + WVR PYP QWG PIF F+    M    L AS  S+                                        I++  T+   V  Y  A+         P V SRGI  EG   +L G+ G+ TG+    EN   + +T++ SRR +   ALF+I FS+  K GA+LASIPL + AA+ C ++A     G S LQY    S R   I+ +SLFLGLSIP YF+ Y+
Sbjct:  191 LPALVLLVIFA---EYASHLFAKGSFVFSRCAVL------VTVVIIWIYAEILTAAGAYNERG------------------PVTQFSCRADRSGIIQGSPWVRFPYPFQWGYPIFCFQDCFAM----LAASFASL----------------------------------------IESTGTLIA-VSRYSGATFC------PPSVFSRGIGWEGISIILDGMCGTLTGTAASVENAGLLAVTRVGSRRVIKISALFMIFFSLFAKFGAVLASIPLPIFAALYCVLFAYSAGAGFSLLQYCNLNSLRTKFILSISLFLGLSIPQYFRVYE 441          

HSP 2 Score: 119.013 bits (297), Expect = 2.428e-26
Identity = 62/155 (40.00%), Postives = 96/155 (61.94%), Query Frame = 3

Query: 1122 GLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTE--HKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            G QH+L + G+ + I +++VP MGG   + A VI T+L +SGI+T+L  +FG+RLP V   S+ Y+ PA+ II +  Y  L +   +F   MR LQGA+I+  +FQ+++GF G+  + +R ++P+   P V   GL  F + FP    CIEV +P
Sbjct:   38 GFQHYLVMLGTTVLIATIIVPLMGGGHAEKAIVIQTILFLSGINTLLQVHFGTRLPAVMSGSYTYIYPAVAIILSPRYALLIDPLERFVFTMRSLQGALIIAGVFQAVVGFFGIWRVFIRFLSPLAAVPFVTLTGLGLFFFAFPGVTKCIEVGLP 192          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT10_ARATH (Putative nucleobase-ascorbate transporter 10 OS=Arabidopsis thaliana GN=NAT10 PE=3 SV=2)

HSP 1 Score: 116.701 bits (291), Expect = 1.406e-25
Identity = 89/288 (30.90%), Postives = 125/288 (43.40%), Query Frame = 1

Query: 2521 LSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQY 3384
            L + + W++A  LT+ G YD+K  +                  T   CR D +       W+ IPYP QWG P F    S  M+  S V                                                   T+ +  G +++++   S+ P  P VVSRG    G   LL G+ G  TG TT TEN   + +TK+ SRR +   A F+I FS+ GK GA  ASIPL + A++ C +   V +VGLS LQ+    SF    I+G S F+ +SIP YF++Y
Sbjct:  238 LCIPLVWLFAQLLTSSGVYDHKSHT------------------TQTSCRTDRTGLITNTPWIYIPYPFQWGSPTFDITDSFAMMAASFV---------------------------------------------------TLFESTGLFYASARYGSATPIPPSVVSRGTCWLGVGVLLNGMLGGITGITTSTENVGLLAMTKIGSRRVIQISAAFMIFFSIFGKFGAFFASIPLPIMASLYCIVLCFVSSVGLSYLQFCNLNSFNIKFILGFSFFMAISIPQYFREY 456          

HSP 2 Score: 107.071 bits (266), Expect = 1.838e-22
Identity = 60/164 (36.59%), Postives = 96/164 (58.54%), Query Frame = 3

Query: 1095 PGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTE--HKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            P L A++  G QH+L   G  + IPSV+VP MGG   +   VI T+L VSG++T+  S+FG+RLP++  +S+ Y+ P   II +  +   T+   +F   MR +QGA+I+   FQ ++   G+   ++R ++P+ +AP     GL  +  GFP    C+EV +P
Sbjct:   39 PWLEAVVL-GFQHYLLSLGITVLIPSVLVPLMGGGYAEKVKVIQTLLFVSGLTTLFQSFFGTRLPVIAVASYAYIIPITSIIYSTRFTYYTDPFERFVRTMRSIQGALIITGCFQVLICILGVWRNIVRFLSPLSIAPLATFTGLGLYHIGFPLLARCVEVGLP 201          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: S23A2_XENTR (Solute carrier family 23 member 2 OS=Xenopus tropicalis GN=slc23a2 PE=2 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 7.787e-25
Identity = 78/288 (27.08%), Postives = 137/288 (47.57%), Query Frame = 1

Query: 2521 LSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAA-WVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQ 3381
            +++ ++W+  F  T                +++   +  K+ Y   + R DA     T A W ++PYP QWG+P       +I ++ ++VAS                                                  I + +G Y++ + L  + PP    ++RGI +EG   +L G++G+G GST+ + N   + ITK+ASRR + +GA F++L  ++GK  A+ AS+P  +  A+ C ++ ++ AVGLS LQ+    S RN+ ++G S+F GL +P+Y +Q
Sbjct:  316 MAILVSWLLCFIFTV---------------TDVFPPDSSKYGY---YARTDARQGVLTVAPWFKVPYPFQWGLPTVS-AAGVIGMLSAVVAS--------------------------------------------------IIESIGDYYACARLSCAPPPPIHAINRGIFIEGLSCVLDGVFGTGNGSTSSSPNIGVLGITKVASRRVIQYGAAFMLLLGMIGKFSALFASLPDPVLGALFCTLFGMITAVGLSNLQFVDLNSSRNLFVLGFSIFFGLMLPSYLKQ 534          

HSP 2 Score: 87.8113 bits (216), Expect = 3.469e-16
Identity = 60/174 (34.48%), Postives = 94/174 (54.02%), Query Frame = 3

Query: 1083 LRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAM--GGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRN--------------LTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAG 1556
            + D P     I+ GLQH+L+     + +P ++  AM  G     T+ +I T+    GI+T+  + FG RLPL Q S+F +LAPA  I++   ++                TEH +   +RE+QGAII+ S+ + ++GF GL   LL+ I P+ + PTV+ +GL+    GF  AG
Sbjct:   92 VEDVPPWYLCIFLGLQHYLTCFSGTVAVPFLLAEAMCVGFDQWATSQLIGTIFFCVGITTLFQTTFGCRLPLFQASAFAFLAPARAILSLEKWKCNTTDLSITNGTELLHTEHIWYPRIREIQGAIIMSSLIEVVIGFLGLPGALLKYIGPLTITPTVSLIGLS----GFQAAG 261          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: S23A2_MOUSE (Solute carrier family 23 member 2 OS=Mus musculus GN=Slc23a2 PE=1 SV=2)

HSP 1 Score: 105.531 bits (262), Expect = 1.146e-21
Identity = 76/299 (25.42%), Postives = 135/299 (45.15%), Query Frame = 1

Query: 2488 RHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNA-WRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQ 3381
            +  + +   + L++ ++W+  F  T                +++       + Y   + R DA       A W ++PYP QWG+P       +I ++ ++VAS                                                  I + +G Y++ + L  + PP    ++RGI +EG   +L GI+G+G GST+ + N   + ITK+ SRR + +GA  ++   +VGK  A+ AS+P  +  A+ C ++ ++ AVGLS LQ+    S RN+ ++G S+F GL +P+Y +Q
Sbjct:  305 KFQLFKMFPIILAILVSWLLCFIFTV---------------TDVFPSNSTDYGY---YARTDARKGVLLVAPWFKVPYPFQWGMPTVS-AAGVIGMLSAVVAS--------------------------------------------------IIESIGDYYACARLSCAPPPPIHAINRGIFVEGLSCVLDGIFGTGNGSTSSSPNIGVLGITKVGSRRVIQYGAALMLGLGMVGKFSALFASLPDPVLGALFCTLFGMITAVGLSNLQFIDLNSSRNLFVLGFSIFFGLVLPSYLRQ 534          

HSP 2 Score: 79.337 bits (194), Expect = 1.483e-13
Identity = 44/135 (32.59%), Postives = 72/135 (53.33%), Query Frame = 3

Query: 1083 LRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAM--GGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYR-------------NLTEHKFRHIMRELQGAIIVGSIFQSIL 1442
            + D P     I+ GLQH+L+     I +P ++  AM  G     T+ +I T+    GI+T+L + FG RLPL Q S+F +LAPA  I++   ++              L EH +   ++E+QGAII+ S+ + ++
Sbjct:   93 IEDVPPWYLCIFLGLQHYLTCFSGTIAVPFLLADAMCVGDDQWATSQLIGTIFFCVGITTLLQTTFGCRLPLFQASAFAFLAPARAILSLDKWKCNTTEITVANGTAELLEHIWHPRIQEIQGAIIMSSLIEVVI 227          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT9_ARATH (Putative nucleobase-ascorbate transporter 9 OS=Arabidopsis thaliana GN=NAT9 PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.843e-21
Identity = 58/153 (37.91%), Postives = 93/153 (60.78%), Query Frame = 3

Query: 1095 PGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTE--HKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFP 1547
            P L A++  G QH+L   G  + IPS++VP MGG D +   VI T+L VSG++T+  S+FG+RLP++  +S+ Y+ P   II +  +   T+   +F   MR +QGA+I+   FQ ++ F G+   ++R ++P+ +AP V   GL  +  GFP
Sbjct:   51 PWLEAVVL-GFQHYLLSLGITVLIPSLLVPLMGGGDAEKVKVIQTLLFVSGLTTLFQSFFGTRLPVIASASYAYIIPITSIIYSTRFTYYTDPFERFVRTMRSIQGALIITGCFQVLVCFLGVWRNIVRFLSPLSIAPLVTFTGLGLYHIGFP 202          

HSP 2 Score: 62.7734 bits (151), Expect = 1.218e-8
Identity = 39/95 (41.05%), Postives = 58/95 (61.05%), Query Frame = 1

Query: 3100 ENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQY 3384
            +N   + +TK+ SRR +   A F++ FS+ GK GA  ASIPL + A++ C +   V + GLS LQ+    SF    I+G S F+ +SIP YF++Y
Sbjct:  299 KNVGLLAMTKVGSRRVIQISAAFMLFFSIFGKFGAFFASIPLPIMASLYCIVLCFVSSAGLSFLQFCNLNSFNTKFILGFSFFMAISIPQYFREY 393          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: S23A2_HUMAN (Solute carrier family 23 member 2 OS=Homo sapiens GN=SLC23A2 PE=1 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 2.187e-21
Identity = 75/288 (26.04%), Postives = 132/288 (45.83%), Query Frame = 1

Query: 2521 LSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNA-WRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQ 3381
            L++ ++W+  F  T                +++   +  K+ +   + R DA       A W ++PYP QWG+P       +I ++ ++VAS                                                  I + +G Y++ + L  + PP    ++RGI +EG   +L GI+G+G GST+ + N   + ITK+ SRR +  GA  ++   ++GK  A+ AS+P  +  A+ C ++ ++ AVGLS LQ+    S RN+ ++G S+F GL +P+Y +Q
Sbjct:  318 LAILVSWLLCFIFTV---------------TDVFPPDSTKYGF---YARTDARQGVLLVAPWFKVPYPFQWGLPTVS-AAGVIGMLSAVVAS--------------------------------------------------IIESIGDYYACARLSCAPPPPIHAINRGIFVEGLSCVLDGIFGTGNGSTSSSPNIGVLGITKVGSRRVIQCGAALMLALGMIGKFSALFASLPDPVLGALFCTLFGMITAVGLSNLQFIDLNSSRNLFVLGFSIFFGLVLPSYLRQ 536          

HSP 2 Score: 78.1814 bits (191), Expect = 3.139e-13
Identity = 46/137 (33.58%), Postives = 74/137 (54.01%), Query Frame = 3

Query: 1083 LRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMG-GTDK-DTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRN---------------LTEHKFRHIMRELQGAIIVGSIFQSIL 1442
            + D P     I+ GLQH+L+     I +P ++  AM  G D+  T+ +I T+    GI+T+L + FG RLPL Q S+F +LAPA  I++   ++                 TEH +   +RE+QGAII+ S+ + ++
Sbjct:   93 IEDVPPWYLCIFLGLQHYLTCFSGTIAVPFLLADAMCVGYDQWATSQLIGTIFFCVGITTLLQTTFGCRLPLFQASAFAFLAPARAILSLDKWKCNTTDVSVANGTAELLHTEHIWYPRIREIQGAIIMSSLIEVVI 229          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: S23A2_RAT (Solute carrier family 23 member 2 OS=Rattus norvegicus GN=Slc23a2 PE=2 SV=2)

HSP 1 Score: 104.375 bits (259), Expect = 2.323e-21
Identity = 74/288 (25.69%), Postives = 131/288 (45.49%), Query Frame = 1

Query: 2521 LSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNA-WRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQ 3381
            L++ ++W+  F  T                +++       + Y   + R DA       A W ++PYP QWG+P       +I ++ ++VAS                                                  I + +G Y++ + L  + PP    ++RGI +EG   +L G++G+G GST+ + N   + ITK+ SRR + +GA  ++   ++GK  A+ AS+P  +  A+ C ++ ++ AVGLS LQ+    S RN+ ++G S+F GL +P+Y +Q
Sbjct:  315 LAILVSWLLCFIFTV---------------TDVFPSNSTDYGY---YARTDARKGVLLVAPWFKVPYPFQWGMPTVS-AAGVIGMLSAVVAS--------------------------------------------------IIESIGDYYACARLSCAPPPPIHAINRGIFVEGLSCVLDGVFGTGNGSTSSSPNIGVLGITKVGSRRVIQYGAALMLGLGMIGKFSALFASLPDPVLGALFCTLFGMITAVGLSNLQFIDLNSSRNLFVLGFSIFFGLVLPSYLRQ 533          

HSP 2 Score: 79.337 bits (194), Expect = 1.506e-13
Identity = 44/135 (32.59%), Postives = 72/135 (53.33%), Query Frame = 3

Query: 1083 LRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAM--GGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYR-------------NLTEHKFRHIMRELQGAIIVGSIFQSIL 1442
            + D P     I+ GLQH+L+     I +P ++  AM  G     T+ +I T+    GI+T+L + FG RLPL Q S+F +LAPA  I++   ++              L EH +   ++E+QGAII+ S+ + ++
Sbjct:   92 IEDVPPWYLCIFLGLQHYLTCFSGTIAVPFLLADAMCVGDDQWATSQLIGTIFFCVGITTLLQTTFGCRLPLFQASAFAFLAPARAILSLDKWKCNTTEITVANGTAELLEHIWHPRIQEIQGAIIMSSLIEVVI 226          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: S23A1_HUMAN (Solute carrier family 23 member 1 OS=Homo sapiens GN=SLC23A1 PE=1 SV=3)

HSP 1 Score: 102.064 bits (253), Expect = 1.028e-20
Identity = 51/145 (35.17%), Postives = 90/145 (62.07%), Query Frame = 1

Query: 2944 ITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQ 3378
            I + +G Y++ + L  + PP    ++RGI  EG C ++AG+ G+G GST+ + N   + ITK+ SRR + +GA  +++   +GK  A+ +S+P  +   + C ++ ++ AVGLS LQ+    S RN+ ++G S+F GL++P Y +
Sbjct:  332 IIESIGDYYACARLAGAPPPPVHAINRGIFTEGICCIIAGLLGTGNGSTSSSPNIGVLGITKVGSRRVVQYGAAIMLVLGTIGKFTALFSSLPDPILGGMFCTLFGMITAVGLSNLQFVDMNSSRNLFVLGFSMFFGLTLPNYLE 476          

HSP 2 Score: 63.5438 bits (153), Expect = 1.088e-8
Identity = 57/175 (32.57%), Postives = 89/175 (50.86%), Query Frame = 3

Query: 1083 LRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMG-GTDKDTAS-VISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNL--------------TEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGS 1559
            + D P     I  G QH+L+     I +P ++  A+  G D+   S +I T+    GI+T++ +  G RLPL Q S+F +L PA  I+    ++                T H +   +RE+QGAI+V S+ + ++G  GL   LL  I P+ V PTV+ +GL+ F     +AGS
Sbjct:   35 IEDVPPWYLCILLGFQHYLTCFSGTIAVPFLLAEALCVGHDQHMVSQLIGTIFTCVGITTLIQTTVGIRLPLFQASAFAFLVPAKAILALERWKCPPEEEIYGNWSLPLNTSHIWHPRIREVQGAIMVSSVVEVVIGLLGLPGALLNYIGPLTVTPTVSLIGLSVFQAAGDRAGS 209          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: S23A1_MOUSE (Solute carrier family 23 member 1 OS=Mus musculus GN=Slc23a1 PE=1 SV=2)

HSP 1 Score: 101.293 bits (251), Expect = 2.006e-20
Identity = 52/145 (35.86%), Postives = 89/145 (61.38%), Query Frame = 1

Query: 2944 ITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQ 3378
            I + +G Y++ + L  + PP    ++RGI  EG C ++AG+ G+G GST+ + N   + ITK+ SRR + +GA  +++   +GK  A+ AS+P  +   + C ++ ++ AVGLS LQ+    S RN+ ++G S+F GL++P Y  
Sbjct:  339 IIESIGDYYACARLAGAPPPPVHAINRGIFTEGICCIIAGLLGTGNGSTSSSPNIGVLGITKVGSRRVVQYGAGIMLILGAIGKFTALFASLPDPILGGMFCTLFGMITAVGLSNLQFVDMNSSRNLFVLGFSMFFGLTLPNYLD 483          

HSP 2 Score: 73.9442 bits (180), Expect = 6.867e-12
Identity = 72/243 (29.63%), Postives = 123/243 (50.62%), Query Frame = 3

Query: 1083 LRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMG-GTDKDTAS-VISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTE--------------HKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXR-------------GISIFGHRLFRIYAV--RTLTYHLVYTF*TLTSIL 1718
            + D P     I  G QH+L+     I +P ++  A+  G D+   S +I T+    GI+T++ +  G RLPL Q S+F +L PA  I+    ++  +E              H +   +RE+QGAI+V S+ + ++G  GL   LL  I P+ V PTV+ +GL+ F     +AGS   +S   +LL+++ + YLR             G+++F  ++F+++ +    +T  L+    TLT +L
Sbjct:   42 IEDVPPWYLCILLGFQHYLTCFSGTIAVPFLLAEALCVGRDQHMVSQLIGTIFTCVGITTLIQTTVGIRLPLFQASAFAFLVPAKSILALERWKCPSEEEIYGNWSMPLNTSHIWHPRIREVQGAIMVSSMVEVVIGLMGLPGALLSYIGPLTVTPTVSLIGLSVFQAAGDRAGSHWGISACSILLIVLFSQYLRNLTFLLPVYRWGKGLTLFRVQIFKMFPIVLAIMTVWLLCYVLTLTDVL 284          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: S23A1_RAT (Solute carrier family 23 member 1 OS=Rattus norvegicus GN=Slc23a1 PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.639e-20
Identity = 52/145 (35.86%), Postives = 89/145 (61.38%), Query Frame = 1

Query: 2944 ITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQ 3378
            I + +G Y++ + L  + PP    ++RGI  EG C ++AG+ G+G GST+ + N   + ITK+ SRR + +GA  +++   +GK  A+ AS+P  +   + C ++ ++ AVGLS LQ+    S RN+ ++G S+F GL++P Y  
Sbjct:  339 IIESIGDYYACARLAGAPPPPVHAINRGIFTEGVCCIIAGLLGTGNGSTSSSPNIGVLGITKVGSRRVVQYGAGIMLILGAIGKFTALFASLPDPILGGMFCTLFGMITAVGLSNLQFVDMNSSRNLFVLGFSMFFGLTLPNYLD 483          

HSP 2 Score: 63.5438 bits (153), Expect = 9.394e-9
Identity = 72/243 (29.63%), Postives = 122/243 (50.21%), Query Frame = 3

Query: 1083 LRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMG-GTDKDTAS-VISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNL--------------TEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXR-------------GISIFGHRLFRIYAV--RTLTYHLVYTF*TLTSIL 1718
            + D P     I  G QH+L+     I +P ++  A+  G D+   S +I T+    GI+T++ +  G RLPL Q S+F +L PA  I+    ++                T H +   +RE+QGAI+V S+ + ++G  GL   LL  I P+ V PTV+ +GL+ F     +AGS   +S   +LL+++ + YLR             G+++F  ++F+++ +    +T  L+    TLT +L
Sbjct:   42 IEDVPPWYLCILLGFQHYLTCFSGTIAVPFLLAEALCVGRDQHMISQLIGTIFTCVGITTLIQTTVGIRLPLFQASAFAFLVPAKAILALERWKCPPEEEIYGNWSMPLNTSHIWHPRIREVQGAIMVSSVVEVVIGLLGLPGALLSYIGPLTVTPTVSLIGLSVFQAAGDRAGSHWGISACSILLIVLFSQYLRNLTFLLPVYRWGKGLTLFRIQIFKMFPIVLAIMTVWLLCYVLTLTDVL 284          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: B9T6T6_RICCO (Purine permease, putative OS=Ricinus communis GN=RCOM_0270650 PE=4 SV=1)

HSP 1 Score: 432.95 bits (1112), Expect = 2.855e-130
Identity = 231/342 (67.54%), Postives = 260/342 (76.02%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQ 3471
            ++ +L+L GI   G H + R   VPLSV I W YAFFLTAGGAY++KGCSP+IPSSNILVD CRKHAYTM+ CR D SNAWRT+AWVRIPYPLQWG+PIFH RTSLIMIIVSLVASVDS                                                   VGTYHS SLLV+SKPPTPG+VSRGIA+EGFCS+LAG+WGSGTGSTTLTEN HTINITK+ASRRA+V GA FLILFS VGKVGAILASIPL+LAA ILCFMW L+ A+GLSTLQY+QTASFRNI IVGVSLFLG+SIP YFQQYQPE+ L+LPSYFVPY+AAS+GPVHT ++Q
Sbjct:  394 LIFTLYLRGISIFG-HRLFRVYAVPLSVVIIWTYAFFLTAGGAYNFKGCSPDIPSSNILVDSCRKHAYTMQRCRTDVSNAWRTSAWVRIPYPLQWGIPIFHLRTSLIMIIVSLVASVDS---------------------------------------------------VGTYHSTSLLVNSKPPTPGIVSRGIAMEGFCSVLAGLWGSGTGSTTLTENVHTINITKVASRRAVVIGAFFLILFSFVGKVGAILASIPLALAAGILCFMWGLIAALGLSTLQYSQTASFRNIAIVGVSLFLGMSIPAYFQQYQPETSLILPSYFVPYSAASNGPVHTSSKQ 683          

HSP 2 Score: 305.449 bits (781), Expect = 4.458e-85
Identity = 170/209 (81.34%), Postives = 198/209 (94.74%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAVRTLTYHLVYTF 1697
            ++ GLRDNPG   LIYYGLQH+LSLAGSLIFIP ++VPAMGGTDKDTA VIST+LL+SGI+TILHSYFG+RLPLVQGSSF++LAPAL+I+NA++YRNL+EHKFRHIMRELQGAIIVGSIFQSI+GF+GLMSLLLRLINPVVVAPTVAAVGLAFFSYGFPQAGSC+E+S+PL+LLVLI TLYLRGISIFGHRLFR+YAV  L+  +++T+
Sbjct:  219 MRFGLRDNPGFVPLIYYGLQHYLSLAGSLIFIPLIIVPAMGGTDKDTAIVISTILLISGITTILHSYFGTRLPLVQGSSFVFLAPALIIMNAQEYRNLSEHKFRHIMRELQGAIIVGSIFQSIMGFTGLMSLLLRLINPVVVAPTVAAVGLAFFSYGFPQAGSCVEISIPLILLVLIFTLYLRGISIFGHRLFRVYAV-PLSVVIIWTY 426          

HSP 3 Score: 137.887 bits (346), Expect = 8.361e-30
Identity = 74/120 (61.67%), Postives = 85/120 (70.83%), Query Frame = 3

Query:  450 METGSNSDSLDKAET----SNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMD 797
            METGS+S+S  K E     +N    KL SMLPKIEPFVP+ D + NPRELRSWAKRTGFVS  S ET  AS++E+FD+  GF + K     + GGSSPKIEIDPVLGRTRP  G EIE D
Sbjct:    1 METGSSSNSASKKEKQKEGANKTKTKLASMLPKIEPFVPRTD-HLNPRELRSWAKRTGFVSTFSSETA-ASNSEKFDTRAGFDLEKGPDHHKNGGSSPKIEIDPVLGRTRPARGSEIESD 118          

HSP 4 Score: 85.5001 bits (210), Expect = 1.706e-13
Identity = 48/61 (78.69%), Postives = 54/61 (88.52%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            Q DF+INAL+S++MVVTLLVAFVLDNTVPG+R ERGVYIWS  ED+ TDPSL  DYSLPGK
Sbjct:  683 QFDFAINALMSLNMVVTLLVAFVLDNTVPGTRQERGVYIWSHPEDLVTDPSLHADYSLPGK 743          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: V4SFY1_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10024972mg PE=4 SV=1)

HSP 1 Score: 424.091 bits (1089), Expect = 4.803e-127
Identity = 228/359 (63.51%), Postives = 264/359 (73.54%), Query Frame = 1

Query: 2401 LPQFILLNNFGLSRKMVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            +PQ +L+        ++ +L+L GI   G H + R   VPLSV I W YAFFLTAGGAY+YKGCSP+IPSSNIL D C KHAYTMKHCR D SNAWRTAAWVRIPYPLQWG+PIFH RTSLIMIIVSLVASVDS                                                   VGTYH+ASLLV+SKPPTP +VSRGI +EGFCS+LAG+WGSGTGS+TLTEN HTI+ITK+ASRRA+  GA+F+I+FS VGKVGAILASIP +LAA+ILCFMWAL+VA+GLS LQYTQ+ASFRNI IVG SLFLGLSIP YFQQY PE+ L+LPSYFVP+AAAS+GPVHT ++Q+ 
Sbjct:  379 IPQILLV--------LIFALYLRGISVFGHH-LFRIYAVPLSVMIIWAYAFFLTAGGAYNYKGCSPDIPSSNILSDACIKHAYTMKHCRTDVSNAWRTAAWVRIPYPLQWGIPIFHLRTSLIMIIVSLVASVDS---------------------------------------------------VGTYHTASLLVNSKPPTPRIVSRGIGMEGFCSILAGLWGSGTGSSTLTENVHTISITKVASRRAVQLGAVFMIVFSFVGKVGAILASIPQALAASILCFMWALIVAIGLSNLQYTQSASFRNIMIVGASLFLGLSIPAYFQQYHPETSLILPSYFVPFAAASNGPVHTGSKQLD 677          

HSP 2 Score: 302.753 bits (774), Expect = 3.781e-84
Identity = 167/209 (79.90%), Postives = 191/209 (91.39%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAVRTLTYHLVYTF 1697
            L+ GLRDN G   +IYYGLQHFLSLAGSLIFIP ++VPAMGGTDKDTA+VISTMLLV+G++T L SYFG+RLPLVQGSSF+YLAPALVIINAR+YRNL+EHKFRHIMRELQGAIIVGSIFQSILGF+GLMSL LRLINPVVVAPTVAA+GLAFFSYGFPQAGSC+E+S+P +LLVLI  LYLRGIS+FGH LFRIYAV  L+  +++ +
Sbjct:  211 LRFGLRDNRGWVPVIYYGLQHFLSLAGSLIFIPLIIVPAMGGTDKDTATVISTMLLVTGVTTTLQSYFGTRLPLVQGSSFVYLAPALVIINAREYRNLSEHKFRHIMRELQGAIIVGSIFQSILGFTGLMSLFLRLINPVVVAPTVAAIGLAFFSYGFPQAGSCVEISIPQILLVLIFALYLRGISVFGHHLFRIYAV-PLSVMIIWAY 418          

HSP 3 Score: 131.724 bits (330), Expect = 9.013e-28
Identity = 71/116 (61.21%), Postives = 85/116 (73.28%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMD 797
            METGS+SDS DK +T   +G+   SM+P+I PFVP+ +  HNPRELRSWAK+TGFVS  SGET T   + +FDS+ G  V +S    R GGSSPKIEIDPVLGRT+PN G EIE D
Sbjct:    1 METGSSSDSRDKQKTL--KGSDRDSMMPQIGPFVPRTE--HNPRELRSWAKKTGFVSDFSGET-TTDVSGKFDSA-GLDVQRSHEHSRGGGSSPKIEIDPVLGRTKPNRGSEIEPD 110          

HSP 4 Score: 88.9669 bits (219), Expect = 1.353e-14
Identity = 51/61 (83.61%), Postives = 57/61 (93.44%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            QLDF+INAL+SM+MVVTLLVAF+LDNTVPGSR ERGVYIWS AED+A+DPSLQ DYSLP K
Sbjct:  675 QLDFAINALMSMNMVVTLLVAFILDNTVPGSRQERGVYIWSRAEDLASDPSLQADYSLPRK 735          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: W9T112_9ROSA (Nucleobase-ascorbate transporter 11 OS=Morus notabilis GN=L484_026777 PE=4 SV=1)

HSP 1 Score: 422.55 bits (1085), Expect = 2.037e-126
Identity = 222/344 (64.53%), Postives = 260/344 (75.58%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            ++ +L+L G+   G   + R   VPLSV I W YAFFLTA GAYDYKGCSP+IPSSNIL D CRKHAYTM+HCR DASNAWRTAAWVRIPYPLQWG+PIFH RTS+IMI+VSLVASVDS                                                   +GTYH+ S+L++ KPPTPG+VSRGIA+EGFCS+LAG+WGSGTGSTTLTEN HTI +TK+ASRRA+  GA+FLI+FS +GKVGAILASIPL+LAAAILCF+WAL+VA+GLSTLQY+QTAS RNI IVGVSLFLGLS+P YFQQYQPES L+LPSYF+PYAAAS+GPVH+ N+Q+ 
Sbjct:  397 LIFTLYLRGLSIFGNR-LFRVYAVPLSVVIIWTYAFFLTASGAYDYKGCSPDIPSSNILNDGCRKHAYTMQHCRTDASNAWRTAAWVRIPYPLQWGMPIFHMRTSIIMIVVSLVASVDS---------------------------------------------------IGTYHTTSVLINLKPPTPGIVSRGIAMEGFCSILAGLWGSGTGSTTLTENLHTIEVTKVASRRAVELGAVFLIVFSFIGKVGAILASIPLALAAAILCFIWALIVALGLSTLQYSQTASLRNIMIVGVSLFLGLSVPAYFQQYQPESSLILPSYFIPYAAASNGPVHSGNKQID 688          

HSP 2 Score: 295.049 bits (754), Expect = 2.021e-81
Identity = 163/209 (77.99%), Postives = 195/209 (93.30%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAVRTLTYHLVYTF 1697
            +K GLR+NPG   L+ YGLQH+LSLAGSLIFIP ++VP+MGGTDKDTA+VISTMLLVSG++TILHSYFG+RLPL+QGSSF+YLAPALVI+N+++YRNLT+HKFRHIMRELQGAIIVGSIFQ +LGFSGLM L LRLINP+VVAPTVAAVGLAFFSYGFPQAGSC+E+S+PL+LLVLI TLYLRG+SIFG+RLFR+YAV  L+  +++T+
Sbjct:  222 IKCGLRENPGFVPLMCYGLQHYLSLAGSLIFIPLIIVPSMGGTDKDTATVISTMLLVSGVATILHSYFGTRLPLIQGSSFVYLAPALVIMNSQEYRNLTDHKFRHIMRELQGAIIVGSIFQFLLGFSGLMCLFLRLINPIVVAPTVAAVGLAFFSYGFPQAGSCVEISLPLILLVLIFTLYLRGLSIFGNRLFRVYAV-PLSVVIIWTY 429          

HSP 3 Score: 102.449 bits (254), Expect = 1.069e-18
Identity = 61/99 (61.62%), Postives = 69/99 (69.70%), Query Frame = 3

Query:  507 GNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSST--GFHVGK--SGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIE 791
            G  LGSMLP+I+PFVP+ D  HNPRELRSWAKRTGFVS+ S E    + +ER  SST  G  + K   G      GSSPKIEIDP+LGRTRPN G EIE
Sbjct:   21 GGTLGSMLPRIDPFVPRADH-HNPRELRSWAKRTGFVSNFSSE----APSERNGSSTSSGLDLEKGFRGHRGGGSGSSPKIEIDPILGRTRPNRGAEIE 114          

HSP 4 Score: 80.4925 bits (197), Expect = 7.158e-12
Identity = 48/61 (78.69%), Postives = 52/61 (85.25%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            Q+DF+INALLSM+MVVTLLVAFVLDNT PGSR ERGVYIWS  ED+  DPSL  DYSLP K
Sbjct:  686 QIDFAINALLSMNMVVTLLVAFVLDNTAPGSRQERGVYIWSRVEDIRDDPSLLADYSLPRK 746          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A059B835_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H04721 PE=4 SV=1)

HSP 1 Score: 419.468 bits (1077), Expect = 4.581e-126
Identity = 223/344 (64.83%), Postives = 256/344 (74.42%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            ++ SL+L GI   G H + +   VP SV I W YAFFLTAGGA+DYKGCSP+IPSSNILVD CRKHAYTMKHCR D SNA RTAAWVRIPYPLQWGVPIFH RTS+IMI+VSLVASVDS+                                                   GTYHSAS+LV+ KPP+PG+VSRGIALEGFCSLLAG+WGS +GSTTLTEN HTI ITK+A+RR +  GA+FLI FS +GKVGAILASIP +LAA+ILCF+WAL+VA+GLSTLQYTQTASFRN+ IVGVSLFLGLSIP YFQQYQPE+ LMLP Y +PYAAASDGPV++ + QV+
Sbjct:  402 LIFSLYLRGISIFG-HRLFQIYAVPFSVVIIWAYAFFLTAGGAFDYKGCSPDIPSSNILVDSCRKHAYTMKHCRTDVSNAMRTAAWVRIPYPLQWGVPIFHLRTSMIMIVVSLVASVDSI---------------------------------------------------GTYHSASVLVNLKPPSPGIVSRGIALEGFCSLLAGLWGSASGSTTLTENLHTIRITKVANRRVVEVGAVFLIFFSFIGKVGAILASIPQALAASILCFIWALIVALGLSTLQYTQTASFRNMIIVGVSLFLGLSIPAYFQQYQPEASLMLPGYLIPYAAASDGPVNSGSSQVT 693          

HSP 2 Score: 293.123 bits (749), Expect = 2.375e-81
Identity = 161/198 (81.31%), Postives = 188/198 (94.95%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV 1664
            +++GLRDNPG   +IYY LQH+LSLAGSLIFIP +MVP MGG+DKDTA+VISTMLLVSGI+T+L+SYFG+RLPLVQGSSF++LAPALVI+N+R+YRNL EH+FRHIMRELQGAIIVGSIFQS LGF+GL+SLL+RLINPVVVAPTVAAVGLAFFSYGFPQAGSC+E+ +PL+LLVLI +LYLRGISIFGHRLF+IYAV
Sbjct:  227 MRLGLRDNPGFVPIIYYSLQHYLSLAGSLIFIPLIMVPTMGGSDKDTATVISTMLLVSGITTLLNSYFGTRLPLVQGSSFVFLAPALVIMNSREYRNLAEHRFRHIMRELQGAIIVGSIFQSFLGFTGLISLLIRLINPVVVAPTVAAVGLAFFSYGFPQAGSCVEIGIPLILLVLIFSLYLRGISIFGHRLFQIYAV 424          

HSP 3 Score: 124.79 bits (312), Expect = 8.566e-26
Identity = 71/114 (62.28%), Postives = 84/114 (73.68%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIE 791
            ME GS+S ++ KAE   G G +LGSM  KI+PFV + D  HNPRELRSWAK+TGFVS  SGET   SS+ +FDS  GF + +   D+R GGSSPKIEIDPVLGRTR + GVEIE
Sbjct:    1 MEAGSSSKNVGKAEGKVG-GRRLGSMFSKIDPFVGRTD--HNPRELRSWAKKTGFVSTFSGET-NRSSSAKFDSG-GFDLERDA-DRRGGGSSPKIEIDPVLGRTRRDSGVEIE 108          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: M5WJ53_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001860mg PE=4 SV=1)

HSP 1 Score: 418.313 bits (1074), Expect = 5.802e-125
Identity = 228/360 (63.33%), Postives = 264/360 (73.33%), Query Frame = 1

Query: 2401 LPQFILLNNFGLSRKMVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQY-TQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            +PQ +L+        ++ +L+L G+   G H   +   VPLSV I W YAFFLTAGGAYDYKGCSP+IPSSNIL+D CRKHAYTM HCR D SNAWRTAAWVRIPYPLQWG+PIFHFRTS+IMI VSLVASVDS                                                   VGTYH+AS+ ++ +PPTPG+VSRGIALEGFCS+LAGIWGSGTGSTTLTEN HTINITK+A+RRA+  GA+FLI FS++GKVGAILASIPL+LAA++LCF WALVVA+GLSTLQY +Q ASFRNI IVGVSLFLGLSIP YFQQYQPES L+LPSYFVPY AAS+GP+HT  +Q+ 
Sbjct:  383 VPQILLI--------LIFTLYLRGVSIFG-HRFFQIYAVPLSVMIIWTYAFFLTAGGAYDYKGCSPDIPSSNILIDACRKHAYTMTHCRTDVSNAWRTAAWVRIPYPLQWGLPIFHFRTSIIMIFVSLVASVDS---------------------------------------------------VGTYHTASVRINLQPPTPGIVSRGIALEGFCSILAGIWGSGTGSTTLTENVHTINITKVANRRAVQLGAVFLIFFSLIGKVGAILASIPLALAASVLCFTWALVVALGLSTLQYSSQAASFRNIMIVGVSLFLGLSIPAYFQQYQPESSLILPSYFVPYGAASNGPLHTGIKQLD 682          

HSP 2 Score: 305.449 bits (781), Expect = 4.661e-85
Identity = 165/209 (78.95%), Postives = 198/209 (94.74%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAVRTLTYHLVYTF 1697
            +K+GLR+NPG   L+YYGLQH+LSLAGSLIFIP ++VPAMGGTDKDT++VISTMLLVSG++TILHSYFG+RLPLVQGSSF+YLAPALVI+NA++YRNLTEHKFRHIMRELQGAII+GSIFQS+LGFSGLMSLLLRLI+P++VAPTVAAVGL+FFSYGFPQAGSC+E+SVP +LL+LI TLYLRG+SIFGHR F+IYAV  L+  +++T+
Sbjct:  215 MKIGLRENPGFVPLMYYGLQHYLSLAGSLIFIPLIIVPAMGGTDKDTSTVISTMLLVSGMTTILHSYFGTRLPLVQGSSFVYLAPALVIMNAQEYRNLTEHKFRHIMRELQGAIIIGSIFQSLLGFSGLMSLLLRLIHPIIVAPTVAAVGLSFFSYGFPQAGSCVEISVPQILLILIFTLYLRGVSIFGHRFFQIYAV-PLSVMIIWTY 422          

HSP 3 Score: 120.168 bits (300), Expect = 2.939e-24
Identity = 67/116 (57.76%), Postives = 81/116 (69.83%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNG--RGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIE 791
            ME GS+S+ LDKAE   G      +GS+ PKI  FVP++D  HNPRELRSWAKRTGFVS+ SGET T+ S    + S GF +      +  GGSSPKIEIDPVLGRT+P+ G+EIE
Sbjct:    1 MEPGSSSEPLDKAERERGPRSDAAIGSVEPKISAFVPRRD--HNPRELRSWAKRTGFVSNFSGETATSGSVR--NDSAGFGLETGFDGRGGGGSSPKIEIDPVLGRTKPSKGIEIE 112          

HSP 4 Score: 78.5666 bits (192), Expect = 2.717e-11
Identity = 45/62 (72.58%), Postives = 52/62 (83.87%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGKF 3846
            QLDF IN LLS++MVVT L+AFVL+N+VPGSR ERGVYIWS  ED++TDPSL  DYSLP K 
Sbjct:  680 QLDFFINGLLSLNMVVTFLIAFVLENSVPGSRQERGVYIWSRPEDISTDPSLLEDYSLPRKL 741          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A059B8R0_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H04721 PE=4 SV=1)

HSP 1 Score: 418.313 bits (1074), Expect = 9.338e-125
Identity = 222/344 (64.53%), Postives = 255/344 (74.13%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            ++ SL+L GI   G H + +   VP SV I W YAFFLTAGGA+DYKGCSP+IPSSNILVD CRKHAYTMKHCR D SNA RTAAWVRIPYPLQWGVPIFH RTS+IMI+VSLVASVDS                                                   +GTYHSAS+LV+ KPP+PG+VSRGIALEGFCSLLAG+WGS +GSTTLTEN HTI ITK+A+RR +  GA+FLI FS +GKVGAILASIP +LAA+ILCF+WAL+VA+GLSTLQYTQTASFRN+ IVGVSLFLGLSIP YFQQYQPE+ LMLP Y +PYAAASDGPV++ + Q+ 
Sbjct:  402 LIFSLYLRGISIFG-HRLFQIYAVPFSVVIIWAYAFFLTAGGAFDYKGCSPDIPSSNILVDSCRKHAYTMKHCRTDVSNAMRTAAWVRIPYPLQWGVPIFHLRTSMIMIVVSLVASVDS---------------------------------------------------IGTYHSASVLVNLKPPSPGIVSRGIALEGFCSLLAGLWGSASGSTTLTENLHTIRITKVANRRVVEVGAVFLIFFSFIGKVGAILASIPQALAASILCFIWALIVALGLSTLQYTQTASFRNMIIVGVSLFLGLSIPAYFQQYQPEASLMLPGYLIPYAAASDGPVNSGSSQLD 693          

HSP 2 Score: 293.893 bits (751), Expect = 6.826e-81
Identity = 161/198 (81.31%), Postives = 188/198 (94.95%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV 1664
            +++GLRDNPG   +IYY LQH+LSLAGSLIFIP +MVP MGG+DKDTA+VISTMLLVSGI+T+L+SYFG+RLPLVQGSSF++LAPALVI+N+R+YRNL EH+FRHIMRELQGAIIVGSIFQS LGF+GL+SLL+RLINPVVVAPTVAAVGLAFFSYGFPQAGSC+E+ +PL+LLVLI +LYLRGISIFGHRLF+IYAV
Sbjct:  227 MRLGLRDNPGFVPIIYYSLQHYLSLAGSLIFIPLIMVPTMGGSDKDTATVISTMLLVSGITTLLNSYFGTRLPLVQGSSFVFLAPALVIMNSREYRNLAEHRFRHIMRELQGAIIVGSIFQSFLGFTGLISLLIRLINPVVVAPTVAAVGLAFFSYGFPQAGSCVEIGIPLILLVLIFSLYLRGISIFGHRLFQIYAV 424          

HSP 3 Score: 125.176 bits (313), Expect = 8.636e-26
Identity = 71/114 (62.28%), Postives = 84/114 (73.68%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIE 791
            ME GS+S ++ KAE   G G +LGSM  KI+PFV + D  HNPRELRSWAK+TGFVS  SGET  +SS  +FDS  GF + +   D+R GGSSPKIEIDPVLGRTR + GVEIE
Sbjct:    1 MEAGSSSKNVGKAEGKVG-GRRLGSMFSKIDPFVGRTD--HNPRELRSWAKKTGFVSTFSGETNRSSSA-KFDSG-GFDLERDA-DRRGGGSSPKIEIDPVLGRTRRDSGVEIE 108          

HSP 4 Score: 74.7146 bits (182), Expect = 4.394e-10
Identity = 44/63 (69.84%), Postives = 52/63 (82.54%), Query Frame = 1

Query: 3655 TCQLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            + QLDF+INAL+S++MVVTLLVA VLD TVPG+R ERGVYIWS  +D+A DPS   DYSLP K
Sbjct:  689 SSQLDFAINALMSLNMVVTLLVAIVLDTTVPGNRQERGVYIWSQRDDIAADPSSLADYSLPRK 751          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A068TZ79_COFCA (Coffea canephora DH200=94 genomic scaffold, scaffold_7 OS=Coffea canephora GN=GSCOC_T00036664001 PE=4 SV=1)

HSP 1 Score: 413.305 bits (1061), Expect = 5.293e-123
Identity = 223/353 (63.17%), Postives = 257/353 (72.80%), Query Frame = 1

Query: 2401 LPQFILLNNFGLSRKMVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHT 3459
            LPQ +L+        ++ +L+L GI   G H + R   VPLS  I W YAFFLTAGGAY++KGC P+IPSSNIL+D CRKHAYTMKHCR D S+A RTAAWVRIPYPLQWG+PIFH RTS+IM+ VSLVASVDS                                                   VGTYHSAS++++ KPPTPG+VSR I LEGFCSLLAG+WGSGTGSTTLTEN HTI++TK+A+RRA+VFGALF+I FS +GKVGAILASIPL+LAAAILCF WAL+VA+GLSTLQYTQT SFRNI IVG SLFLGLSIP Y QQYQPES L+LPSY +PY+AASDGPVHT
Sbjct:  383 LPQIVLV--------LIFTLYLRGISIFG-HKVFRIYAVPLSAIIIWAYAFFLTAGGAYNFKGCDPDIPSSNILIDACRKHAYTMKHCRTDVSDAMRTAAWVRIPYPLQWGIPIFHMRTSIIMVFVSLVASVDS---------------------------------------------------VGTYHSASVVINLKPPTPGIVSRAIGLEGFCSLLAGLWGSGTGSTTLTENLHTISVTKVANRRAVVFGALFMIFFSFIGKVGAILASIPLALAAAILCFTWALIVALGLSTLQYTQTPSFRNIMIVGASLFLGLSIPAYVQQYQPESSLILPSYLIPYSAASDGPVHT 675          

HSP 2 Score: 296.59 bits (758), Expect = 6.655e-82
Identity = 164/198 (82.83%), Postives = 187/198 (94.44%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV 1664
            +K GLR+NPG   LIYYGLQHFLSLAGSLIFIP ++VP MGG+DKDTA+VISTMLLVSGI+TIL+S+FG+RLPLVQGSSF+YLAPAL I+N+R+YRNLTEH+FRHIMRE+QGAIIVGSI QSILGFSGLMS LLRLINPVVVAPTVAAVGLAFFSYGFPQAGSC+E+S+P ++LVLI TLYLRGISIFGH++FRIYAV
Sbjct:  215 MKCGLRENPGFVPLIYYGLQHFLSLAGSLIFIPLIIVPIMGGSDKDTATVISTMLLVSGITTILNSFFGTRLPLVQGSSFVYLAPALAIMNSREYRNLTEHRFRHIMREIQGAIIVGSICQSILGFSGLMSYLLRLINPVVVAPTVAAVGLAFFSYGFPQAGSCVEISLPQIVLVLIFTLYLRGISIFGHKVFRIYAV 412          

HSP 3 Score: 102.834 bits (255), Expect = 8.926e-19
Identity = 58/116 (50.00%), Postives = 78/116 (67.24%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMD 797
            ME GS+S++ +K +    +G KLGS+LP +EPF+P+K+  ++PRELRSWAK+TGFVS  SGET   S + R   +    +      ++    SPKIEIDP+LGRTR N GVEIE D
Sbjct:    1 MEGGSSSETGEKVDKQ--KGLKLGSVLPNVEPFMPRKN-FYDPRELRSWAKKTGFVSTFSGETDRGSVSGRNVENEREKLDLEKVIEKKESLSPKIEIDPILGRTR-NRGVEIEQD 112          

HSP 4 Score: 70.4774 bits (171), Expect = 8.866e-9
Identity = 41/63 (65.08%), Postives = 50/63 (79.37%), Query Frame = 1

Query: 3655 TCQLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            +   DF+ NALLS++MVVTL+VAFVLDNTVPGSR ERGVY+WS  +D+ TDPS   DY+ P K
Sbjct:  677 SADFDFAFNALLSLNMVVTLVVAFVLDNTVPGSRQERGVYLWSRTDDIMTDPSSLSDYTPPSK 739          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: K7K1D0_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 402.134 bits (1032), Expect = 4.475e-122
Identity = 216/345 (62.61%), Postives = 255/345 (73.91%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPY-AAASDGPVHTINEQVS 3477
            ++ +L+L GI   GRH + R   VPLS+TI WIYA FLTAGGAY+YKGC+P+IPSSNIL+D CRKHAYTMKHCR D SNA  TAAWVR+PYPLQWG+PIFHFRTS+IM+IVSLVASVDS                                                   VGTY + SL V+S+PPTPGVVSRGIALEGFCS+LAG+WGSGTG+TTLTEN HTI++TK+ASR+ +V GA FLI+FS +GKVGA+LASIPL+LAA++LCFMWAL  A+GLS LQY+Q+ASFRNITIVGVSLFLG+SIP YFQQYQ ES L+LPSY VPY AAAS GP  +  +Q+ 
Sbjct:  141 LIFTLYLRGISIFGRH-LFRIYAVPLSLTIIWIYASFLTAGGAYNYKGCNPDIPSSNILLDACRKHAYTMKHCRTDVSNALSTAAWVRMPYPLQWGIPIFHFRTSIIMVIVSLVASVDS---------------------------------------------------VGTYRATSLQVNSRPPTPGVVSRGIALEGFCSILAGLWGSGTGATTLTENMHTIDVTKVASRKVVVVGAAFLIMFSFIGKVGALLASIPLALAASVLCFMWALTAALGLSNLQYSQSASFRNITIVGVSLFLGMSIPAYFQQYQAESSLVLPSYLVPYAAAASSGPFRSGIKQLD 433          

HSP 2 Score: 247.284 bits (630), Expect = 4.594e-67
Identity = 140/173 (80.92%), Postives = 158/173 (91.33%), Query Frame = 3

Query: 1176 MVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV-RTLTYHLVY 1691
            M+P MGGTDKDTA+VISTML +SGI+TILHSY G+RLPLVQGSSF+YLAPALVIINA++YRNLTEHKFRHIMRELQGAIIVGS+FQ ILGFSGLMS+LLRLINP+VVAPTVAAVGLAFFSYGFPQAG+C E+++P + LVLI TLYLRGISIFG  LFRIYAV  +LT   +Y
Sbjct:    1 MIPVMGGTDKDTATVISTMLFLSGITTILHSYLGTRLPLVQGSSFVYLAPALVIINAQEYRNLTEHKFRHIMRELQGAIIVGSVFQCILGFSGLMSILLRLINPIVVAPTVAAVGLAFFSYGFPQAGTCPEITIPQIALVLIFTLYLRGISIFGRHLFRIYAVPLSLTIIWIY 173          

HSP 3 Score: 84.3445 bits (207), Expect = 2.653e-13
Identity = 47/61 (77.05%), Postives = 56/61 (91.80%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            QLDF+INAL+S++MVVTLLVAF+LDNTVPGS++ERGVY WS AED+ATDPS Q +YSLP K
Sbjct:  431 QLDFAINALMSLNMVVTLLVAFLLDNTVPGSQEERGVYQWSRAEDIATDPSQQSEYSLPKK 491          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: I1L4S1_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 408.297 bits (1048), Expect = 1.733e-121
Identity = 217/344 (63.08%), Postives = 255/344 (74.13%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            ++ +L+L GI   GRH + R   VPLS+TI WIYA FLTAGGAY+YKGC+P+IPSSNIL+D CRKHAYTMKHCR D SNA  TAAWVRIPYPLQWG+PIFHFRTS+IM+IVSLVASVDS                                                   VGTY + SL V+S+PPTPGVVSRGIALEGFCS+LAG+WGSGTG+TTLTENTHTI+ITK+ASR+ +V GA F+ILFS +GKVGA+LASIP +LAA++LCFMWAL  A+GLS LQY+++ASFRNITIVGVSLFLG+SIP YFQQYQ ES L+LPSY VPYAAAS GP  +  +Q+ 
Sbjct:  366 LIFTLYLRGISIFGRH-LFRIYAVPLSLTIIWIYASFLTAGGAYNYKGCNPDIPSSNILLDACRKHAYTMKHCRTDVSNALSTAAWVRIPYPLQWGIPIFHFRTSIIMVIVSLVASVDS---------------------------------------------------VGTYRATSLQVNSRPPTPGVVSRGIALEGFCSILAGLWGSGTGATTLTENTHTIDITKVASRKVVVVGAAFVILFSFIGKVGALLASIPQALAASVLCFMWALTAALGLSNLQYSKSASFRNITIVGVSLFLGMSIPAYFQQYQAESSLILPSYLVPYAAASSGPFRSGIKQLD 657          

HSP 2 Score: 298.901 bits (764), Expect = 4.730e-83
Identity = 166/208 (79.81%), Postives = 188/208 (90.38%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV-RTLTYHLVY 1691
            LK GL++NPG+  LIYYGLQH+LSL GSL+ IP VMVP MGGTDKDTA+VIST+L +SGI+TILHSYFG+RLPLVQGSSF+YLAPALVIINA++YRNLTEHKFRHIMRELQGAIIVGS+FQ ILGFSGLMS+LLRLINP+VVAPTVAAVGLAFFSYGFPQAGSC E+++P + LVLI TLYLRGISIFG  LFRIYAV  +LT   +Y
Sbjct:  191 LKCGLKENPGIVPLIYYGLQHYLSLVGSLVLIPLVMVPVMGGTDKDTATVISTILFLSGITTILHSYFGTRLPLVQGSSFVYLAPALVIINAQEYRNLTEHKFRHIMRELQGAIIVGSVFQCILGFSGLMSILLRLINPIVVAPTVAAVGLAFFSYGFPQAGSCPEITIPQIALVLIFTLYLRGISIFGRHLFRIYAVPLSLTIIWIY 398          

HSP 3 Score: 105.916 bits (263), Expect = 8.814e-20
Identity = 70/150 (46.67%), Postives = 89/150 (59.33%), Query Frame = 3

Query:  450 METGSNSDSLDK-----AETSNGRGNKLGSML---PKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDXXXXXXXXXXXKTVADGNGLGEKVEE 875
            METGSNS+SL++        S G+  + G +     K++PFVP+ +  HNPRELRSWAKRTGFVS  SGE G++ S  +F++           ++R GGSSPKIEIDPV+GRTR N   EIE +   G   R     V DG G  EK  E
Sbjct:    1 METGSNSESLNRNVAMSVRGSTGKKKQQGGVTLAGKKVDPFVPRSE--HNPRELRSWAKRTGFVSDYSGEAGSSGSA-KFEA----------LERRGGGSSPKIEIDPVVGRTRQN---EIEQE-THGGAMRGENGAVLDGRGRKEKENE 133          

HSP 4 Score: 85.1149 bits (209), Expect = 2.055e-13
Identity = 47/61 (77.05%), Postives = 57/61 (93.44%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            QLDF+INAL+S++MVVTLLVAF+LDNTVPGS++ERGVY+WS AED+ TDPSLQ +YSLP K
Sbjct:  655 QLDFAINALMSLNMVVTLLVAFLLDNTVPGSQEERGVYLWSQAEDIVTDPSLQSEYSLPKK 715          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A0B2RWT7_GLYSO (Nucleobase-ascorbate transporter 11 OS=Glycine soja GN=glysoja_014873 PE=4 SV=1)

HSP 1 Score: 408.297 bits (1048), Expect = 1.733e-121
Identity = 217/344 (63.08%), Postives = 255/344 (74.13%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            ++ +L+L GI   GRH + R   VPLS+TI WIYA FLTAGGAY+YKGC+P+IPSSNIL+D CRKHAYTMKHCR D SNA  TAAWVRIPYPLQWG+PIFHFRTS+IM+IVSLVASVDS                                                   VGTY + SL V+S+PPTPGVVSRGIALEGFCS+LAG+WGSGTG+TTLTENTHTI+ITK+ASR+ +V GA F+ILFS +GKVGA+LASIP +LAA++LCFMWAL  A+GLS LQY+++ASFRNITIVGVSLFLG+SIP YFQQYQ ES L+LPSY VPYAAAS GP  +  +Q+ 
Sbjct:  366 LIFTLYLRGISIFGRH-LFRIYAVPLSLTIIWIYASFLTAGGAYNYKGCNPDIPSSNILLDACRKHAYTMKHCRTDVSNALSTAAWVRIPYPLQWGIPIFHFRTSIIMVIVSLVASVDS---------------------------------------------------VGTYRATSLQVNSRPPTPGVVSRGIALEGFCSILAGLWGSGTGATTLTENTHTIDITKVASRKVVVVGAAFVILFSFIGKVGALLASIPQALAASVLCFMWALTAALGLSNLQYSKSASFRNITIVGVSLFLGMSIPAYFQQYQAESSLILPSYLVPYAAASSGPFRSGIKQLD 657          

HSP 2 Score: 298.901 bits (764), Expect = 4.730e-83
Identity = 166/208 (79.81%), Postives = 188/208 (90.38%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV-RTLTYHLVY 1691
            LK GL++NPG+  LIYYGLQH+LSL GSL+ IP VMVP MGGTDKDTA+VIST+L +SGI+TILHSYFG+RLPLVQGSSF+YLAPALVIINA++YRNLTEHKFRHIMRELQGAIIVGS+FQ ILGFSGLMS+LLRLINP+VVAPTVAAVGLAFFSYGFPQAGSC E+++P + LVLI TLYLRGISIFG  LFRIYAV  +LT   +Y
Sbjct:  191 LKCGLKENPGIVPLIYYGLQHYLSLVGSLVLIPLVMVPVMGGTDKDTATVISTILFLSGITTILHSYFGTRLPLVQGSSFVYLAPALVIINAQEYRNLTEHKFRHIMRELQGAIIVGSVFQCILGFSGLMSILLRLINPIVVAPTVAAVGLAFFSYGFPQAGSCPEITIPQIALVLIFTLYLRGISIFGRHLFRIYAVPLSLTIIWIY 398          

HSP 3 Score: 105.916 bits (263), Expect = 8.814e-20
Identity = 70/150 (46.67%), Postives = 89/150 (59.33%), Query Frame = 3

Query:  450 METGSNSDSLDK-----AETSNGRGNKLGSML---PKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDXXXXXXXXXXXKTVADGNGLGEKVEE 875
            METGSNS+SL++        S G+  + G +     K++PFVP+ +  HNPRELRSWAKRTGFVS  SGE G++ S  +F++           ++R GGSSPKIEIDPV+GRTR N   EIE +   G   R     V DG G  EK  E
Sbjct:    1 METGSNSESLNRNVAMSVRGSTGKKKQQGGVTLAGKKVDPFVPRSE--HNPRELRSWAKRTGFVSDYSGEAGSSGSA-KFEA----------LERRGGGSSPKIEIDPVVGRTRQN---EIEQE-THGGAMRGENGAVLDGRGRKEKENE 133          

HSP 4 Score: 85.1149 bits (209), Expect = 2.055e-13
Identity = 47/61 (77.05%), Postives = 57/61 (93.44%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            QLDF+INAL+S++MVVTLLVAF+LDNTVPGS++ERGVY+WS AED+ TDPSLQ +YSLP K
Sbjct:  655 QLDFAINALMSLNMVVTLLVAFLLDNTVPGSQEERGVYLWSQAEDIVTDPSLQSEYSLPKK 715