g.barbadense_cottongen_reftransV1_0094223, g.barbadense_cottongen_reftransV1_0094223 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT11_ARATH (Nucleobase-ascorbate transporter 11 OS=Arabidopsis thaliana GN=NAT11 PE=2 SV=1)

HSP 1 Score: 398.667 bits (1023), Expect = 3.106e-120
Identity = 210/329 (63.83%), Postives = 241/329 (73.25%), Query Frame = 1

Query: 2491 HVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            H + R   VPLS  + W YAFFLT GGAYDY+GC+ +IPSSNIL+DEC+KH YTMKHCR DASNAWRTA+WVRIPYP QWG P FH RTS+IMI VSLVASVDS                                                   VGTYHSAS++V++K PT G+VSRGIALEGFCSLLAGIWGSGTGSTTLTEN HTINITK+ASRRALV GA+FLI+ S +GK+GAILASIP +LAA++LCF+WAL V++GLS L+YTQTASFRNITIVGVSLFLGLSIP YFQQYQP S L+LPSY++P+ AAS GP  T  EQ+ 
Sbjct:  364 HRLFRIYAVPLSALLIWTYAFFLTVGGAYDYRGCNADIPSSNILIDECKKHVYTMKHCRTDASNAWRTASWVRIPYPFQWGFPNFHMRTSIIMIFVSLVASVDS---------------------------------------------------VGTYHSASMIVNAKRPTRGIVSRGIALEGFCSLLAGIWGSGTGSTTLTENIHTINITKVASRRALVIGAMFLIVLSFLGKLGAILASIPQALAASVLCFIWALTVSLGLSNLRYTQTASFRNITIVGVSLFLGLSIPAYFQQYQPLSSLILPSYYIPFGAASSGPFQTGIEQLD 641          

HSP 2 Score: 293.123 bits (749), Expect = 4.372e-83
Identity = 163/209 (77.99%), Postives = 190/209 (90.91%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAVRTLTYHLVYTF 1697
            +K GLRDNPG   LIYYGLQH+LSL GSL+FIP V+VPAM G+DKDTASVISTMLL++G++TILH YFG+RLPLVQGSSF+YLAP LV+IN+ ++RNLTEHKFR  MRELQGAIIVGS+FQ ILGFSGLMSLLLR INPVVVAPTVAAVGLAFFSYGFPQAG+C+E+SVPL+LL+LI TLYLRG+S+FGHRLFRIYAV  L+  L++T+
Sbjct:  175 MKFGLRDNPGFVPLIYYGLQHYLSLVGSLVFIPLVIVPAMDGSDKDTASVISTMLLLTGVTTILHCYFGTRLPLVQGSSFVYLAPVLVVINSEEFRNLTEHKFRDTMRELQGAIIVGSLFQCILGFSGLMSLLLRFINPVVVAPTVAAVGLAFFSYGFPQAGTCVEISVPLILLLLIFTLYLRGVSLFGHRLFRIYAV-PLSALLIWTY 382          

HSP 3 Score: 98.9821 bits (245), Expect = 1.313e-19
Identity = 50/88 (56.82%), Postives = 63/88 (71.59%), Query Frame = 3

Query:  513 KLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNM 776
            K G+   ++EPF+PKKD N  PR+LRSWAK+TGFVS  SGET T++ T +F  S+ F + K GRDQ   GSS K EIDP+LGR RP +
Sbjct:   29 KFGAFFKRVEPFLPKKDLN--PRDLRSWAKKTGFVSDYSGETSTSTRT-KFGESSDFDLPK-GRDQVVTGSSHKTEIDPILGRNRPEI 112          

HSP 4 Score: 81.2629 bits (199), Expect = 3.813e-14
Identity = 41/62 (66.13%), Postives = 55/62 (88.71%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGKF 3846
            QLDF++NA+LS++MVVT L+AF+LDNTVPGS++ERGVY+W+ AED+  DP ++ DYSLP KF
Sbjct:  639 QLDFAMNAVLSLNMVVTFLLAFILDNTVPGSKEERGVYVWTRAEDMQMDPEMRADYSLPRKF 700          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT12_ARATH (Nucleobase-ascorbate transporter 12 OS=Arabidopsis thaliana GN=NAT12 PE=1 SV=3)

HSP 1 Score: 266.929 bits (681), Expect = 5.070e-74
Identity = 159/322 (49.38%), Postives = 202/322 (62.73%), Query Frame = 1

Query: 2491 HVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQY--QPESILMLPSYFVPYAAASDGP 3450
            H I     VPLS+ ITW  AF LT  GAY YKGC P +P SN++   CRK+   MK+CR D S+A  +A W R PYPLQWGVP+F+++ + +M +VS++ASVDS                                                   VG+YH++SLLV+S+PPT GVVSR I LEGF S+LAG+WG+GTGSTTLTEN HTI +TKM SRR +  GA  L++FS+VGKVG  LASIP  + A++LCFMWA+  A+GLS L+Y++  S RNI IVG+SLF  LS+P YFQQY   P S L +PSY+ PY  +S GP
Sbjct:  359 HRIFLIYAVPLSLAITWAAAFLLTETGAYTYKGCDPNVPVSNVVSTHCRKYMTRMKYCRVDTSHALSSAPWFRFPYPLQWGVPLFNWKMAFVMCVVSVIASVDS---------------------------------------------------VGSYHASSLLVASRPPTRGVVSRAIGLEGFTSVLAGLWGTGTGSTTLTENVHTIAVTKMGSRRVVELGACVLVIFSLVGKVGGFLASIPQVMVASLLCFMWAMFTALGLSNLRYSEAGSSRNIIIVGLSLFFSLSVPAYFQQYGISPNSNLSVPSYYQPYIVSSHGP 629          

HSP 2 Score: 225.713 bits (574), Expect = 4.567e-60
Identity = 127/199 (63.82%), Postives = 166/199 (83.42%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLT-EHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV 1664
            +K GLRD PGL  + +YGLQH+LS+ GSLI +P V+VPAMGG+ ++ A+V+ST+L VSGI+T+LH+ FGSRLPL+QG SF++LAPAL IIN+ +++ L   + F+HIMRELQGAII+GS FQ++LG+SGLMSL+LRL+NPVVVAPTVAAVGL+F+SYGFP  G C+E+ V  +LLV+I  LYLR IS+  HR+F IYAV
Sbjct:  169 MKYGLRDTPGLVPIGFYGLQHYLSMLGSLILVPLVIVPAMGGSHEEVANVVSTVLFVSGITTLLHTSFGSRLPLIQGPSFVFLAPALAIINSPEFQGLNGNNNFKHIMRELQGAIIIGSAFQAVLGYSGLMSLILRLVNPVVVAPTVAAVGLSFYSYGFPLVGKCLEIGVVQILLVIIFALYLRKISVLSHRIFLIYAV 367          

HSP 3 Score: 58.9214 bits (141), Expect = 3.077e-7
Identity = 33/64 (51.56%), Postives = 47/64 (73.44%), Query Frame = 1

Query: 3664 LDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLP---GKF 3846
            +++ +N LLSMSMV+  ++A +LDNTVPGS+ ERGVY+WS +E    +P+L  DY LP   G+F
Sbjct:  637 MNYVMNTLLSMSMVIAFIMAVILDNTVPGSKQERGVYVWSDSETATREPALAKDYELPFRVGRF 700          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT1_ARATH (Nucleobase-ascorbate transporter 1 OS=Arabidopsis thaliana GN=NAT1 PE=2 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 4.687e-37
Identity = 109/296 (36.82%), Postives = 144/296 (48.65%), Query Frame = 1

Query: 2497 IVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQY 3384
            I     + + VTI WIYA  LTA GAY  +G                K + T   CR D +N   TA W + PYPLQWG P F    S  M+   LV+ V                                                   +  G Y +AS L  + PP   V+SRGI  +G   LL G++G+GTGST L EN   + +T++ SRR +   A F+I+FS +GK GA+ ASIP+ + AA+ C ++ LV AVGLS LQ+T   S RN+ I G+SLFLG+SIP +F QY
Sbjct:  209 IFERFPILICVTIVWIYAVILTASGAY--RG----------------KPSLTQHSCRTDKANLISTAPWFKFPYPLQWGPPTFSVGHSFAMMSAVLVSMV---------------------------------------------------ESTGAYIAASRLAIATPPPAYVLSRGIGWQGIGVLLDGLFGTGTGSTVLVENVGLLGLTRVGSRRVVQVSAGFMIVFSTLGKFGAVFASIPVPIYAALHCILFGLVAAVGLSFLQFTNMNSMRNLMITGLSLFLGISIPQFFAQY 435          

HSP 2 Score: 128.257 bits (321), Expect = 2.347e-29
Identity = 73/172 (42.44%), Postives = 105/172 (61.05%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLT--EHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            L+  +  NP     +    Q+++ + G+  FIP+++VPAMGG+D D A VI T+L V+GI T+L + FG+RLP V G S  Y+ P   IIN    + ++    +F H MR +QGA+IV S  Q ILG+S +  L  R  +P+ +AP V  VGL  F  GFPQ G+CIE+ +P
Sbjct:   15 LEYCIDSNPPWPETVLLAFQNYILMLGTSAFIPALLVPAMGGSDGDRARVIQTLLFVAGIKTLLQALFGTRLPAVVGGSLAYVVPIAYIINDSSLQKISNDHERFIHTMRAIQGALIVASSIQIILGYSQVWGLFSRFFSPLGMAPVVGLVGLGMFQRGFPQLGNCIEIGLP 186          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT5_ARATH (Nucleobase-ascorbate transporter 5 OS=Arabidopsis thaliana GN=NAT5 PE=2 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 6.971e-37
Identity = 109/325 (33.54%), Postives = 151/325 (46.46%), Query Frame = 1

Query: 2485 GRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHT 3459
            G+HV  R   V  SV I W+YAFFLT GGAY+  G                    T + CR D +     A W+R+P+P QWG P+F    +  M++ S VA V+S   +                                               V  Y SA++      P P V+SRG+  +G   L++G++G+G GS+   EN   + +TK+ SRR +   A F+I FS++GK GA+ ASIP  + AA+ C  +A V A GLS LQ+    SFR + I+G S+FLGLSIP YF ++               A    GPVHT
Sbjct:  212 GKHVFAR-FAVIFSVAIVWLYAFFLTLGGAYNGVGTD------------------TQRSCRTDRAGLISAAPWIRVPWPFQWGAPLFDAGEAFAMMMASFVALVESTGAFIA---------------------------------------------VSRYASATM------PPPSVISRGVGWQGVAILISGLFGTGIGSSVSVENAGLLALTKIGSRRVVQISAGFMIFFSILGKFGAVFASIPSPIIAALYCLFFAYVGAGGLSLLQFCNLNSFRTLFILGFSIFLGLSIPQYFNEHT--------------AIKGYGPVHT 452          

HSP 2 Score: 111.694 bits (278), Expect = 5.654e-24
Identity = 60/151 (39.74%), Postives = 97/151 (64.24%), Query Frame = 3

Query: 1122 GLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTE--HKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIE 1568
            G QH+L + G+ + IPS +VP MGG +++ A +I T+L V+G++T+L + FG+RLP V G+S+ ++   + I+ +  + ++ +   +F+ I+R  QGA+IV S  Q ILGFSGL   ++R ++P+  AP V  VG   +  GFP    CIE
Sbjct:   39 GFQHYLVMLGTTVLIPSALVPQMGGRNEEKAKLIQTILFVAGLNTLLQTVFGTRLPAVIGASYTFVPVTISIMLSGRFNDVADPVERFKRIIRATQGALIVASTLQIILGFSGLWRNVVRFLSPLSAAPLVGLVGYGLYELGFPGVAKCIE 189          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT2_ARATH (Nucleobase-ascorbate transporter 2 OS=Arabidopsis thaliana GN=NAT2 PE=2 SV=2)

HSP 1 Score: 149.443 bits (376), Expect = 5.460e-36
Identity = 106/321 (33.02%), Postives = 153/321 (47.66%), Query Frame = 1

Query: 2497 IVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHT 3459
            +V    + +++ I W YA  LTA GAY +                 R H  T  +CR D SN   +A W++IPYPLQWG P F    +  M+   LV                                                   ++ +  G + +A+ L S+ PP P V+SRGI  +G   LL G++G+ +GS+   EN   +  T++ SRR +   A F+I FS++GK GA+ ASIP ++ AA+ C ++ LV +VGLS LQ+T   S RN+ IVGVSLFLGLSIP YF+ +  +++               GP HT
Sbjct:  213 VVERFALIIALIIVWAYAHVLTASGAYKH-----------------RPHQ-TQLNCRTDMSNLISSAPWIKIPYPLQWGAPSFDAGHAFAMMAAVLV---------------------------------------------------SLIESTGAFKAAARLASATPPPPHVLSRGIGWQGIGILLNGLFGTLSGSSVSVENIGLLGSTRVGSRRVIQISAGFMIFFSMLGKFGALFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSLRNLFIVGVSLFLGLSIPEYFRDFSMKAL--------------HGPAHT 450          

HSP 2 Score: 120.553 bits (301), Expect = 7.278e-27
Identity = 64/172 (37.21%), Postives = 103/172 (59.88%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHI--MRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            L+  +  NP  G  I  G +H++   G+ + IPS++VP MGG D D   V+ T+L + G++T+L + FG+RLP V G S+ ++ P + II+      + + + R +  MR +QGAIIV S  Q ILGFS + ++  R  +P+ + P +A  G   F+ GFP  G+C+E+ +P
Sbjct:   20 LEYCIDSNPPWGEAIALGFEHYILALGTAVMIPSILVPMMGGDDGDKVRVVQTLLFLQGVNTLLQTLFGTRLPTVIGGSYAFMVPIISIIHDSSLTRIEDPQLRFLSTMRAVQGAIIVASSVQIILGFSQMWAICSRFFSPIGMVPVIALTGFGLFNRGFPVVGNCVEIGLP 191          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT7_ARATH (Nucleobase-ascorbate transporter 7 OS=Arabidopsis thaliana GN=NAT7 PE=2 SV=2)

HSP 1 Score: 149.058 bits (375), Expect = 6.400e-36
Identity = 108/325 (33.23%), Postives = 142/325 (43.69%), Query Frame = 1

Query: 2485 GRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHT 3459
            G   +     V  SV I WIYA  LT GGAY   G +                  T   CR D S     + W+R+PYP QWG P FH   +  M+ VS V                                                   ++ +  GTY   S   S+ PP P V+SRG+  +G   LL G++G+G G++   EN   + +T++ SRR +   A F+I FS++GK GAI ASIP  + AA+ C  +A V A GLS LQ+    SFR   I+G S+F+GLSIP YF QY               A    GPVHT
Sbjct:  221 GERQVFHRFAVIFSVVIVWIYAHLLTVGGAYKNTGVN------------------TQTSCRTDRSGLISGSPWIRVPYPFQWGPPTFHAGEAFAMMAVSFV---------------------------------------------------SLIESTGTYIVVSRFASATPPPPSVLSRGVGWQGVGVLLCGLFGAGNGASVSVENAGLLALTRVGSRRVVQISAGFMIFFSILGKFGAIFASIPAPVVAALHCLFFAYVGAGGLSLLQFCNLNSFRTKFILGFSVFMGLSIPQYFNQYT--------------AVNKYGPVHT 462          

HSP 2 Score: 129.028 bits (323), Expect = 1.658e-29
Identity = 68/158 (43.04%), Postives = 104/158 (65.82%), Query Frame = 3

Query: 1113 IYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLT--EHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            I  G QH+L + G+ + IP+ +VP MGG +++ A ++ T+L VSG++T+L S+FG+RLP V G S+ Y+   L II A  Y ++   + KF+ IMR +QGA+IV SI Q ++GFSGL   ++RL++P+   P VA  G   + +GFP    CIE+ +P
Sbjct:   46 ILLGFQHYLVMLGTTVLIPTYLVPQMGGGNEEKAKMVQTLLFVSGLNTLLQSFFGTRLPAVIGGSYTYVPTTLSIILAGRYSDILDPQEKFKRIMRGIQGALIVASILQIVVGFSGLWRNVVRLLSPLSAVPLVALAGFGLYEHGFPLLAKCIEIGLP 203          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT4_ARATH (Nucleobase-ascorbate transporter 4 OS=Arabidopsis thaliana GN=NAT4 PE=2 SV=2)

HSP 1 Score: 140.198 bits (352), Expect = 4.670e-33
Identity = 103/302 (34.11%), Postives = 140/302 (46.36%), Query Frame = 1

Query: 2479 FVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQY 3384
            F  +  I     V  ++ I W YA  LTA GAYD                  ++   T   CR D S     + WVRIPYPLQWG P FH   +  M+  + VA                                                   I +  G++ +AS   S+    P V+SRGI  +G   LL G++G+ TGST L ENT  + +TK+ SRR +   A F+I FS+ GK GA+LASIPL + AA+ C ++A V + GL  LQ+    SFRN  I+G S+F+GLS+  YF +Y
Sbjct:  207 FKCKRSICEQFAVLFTIAIVWAYAEILTAAGAYD------------------KRPDNTQLSCRTDRSGLISASPWVRIPYPLQWGRPSFHGSDAFAMMAATYVA---------------------------------------------------IVETTGSFIAASRFGSATHIPPSVLSRGIGWQGIGVLLNGLFGTATGSTALVENTGLLGLTKVGSRRVVQISAGFMIFFSIFGKFGAVLASIPLPIFAALYCVLFAYVASAGLGLLQFCNLNSFRNKFILGFSIFIGLSVAQYFTEY 439          

HSP 2 Score: 126.716 bits (317), Expect = 8.267e-29
Identity = 71/154 (46.10%), Postives = 100/154 (64.94%), Query Frame = 3

Query: 1113 IYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVI-INARDYRNLTEH-KFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIE 1568
            I  G QH++ + G+ + IPS++VP MGG D + A VI+T+L VSGI+T+L S FGSRLP+V G+S+ YL PAL I  + R    L  H +F   MR +QGA+I+ SI   I+GF GL  +L+R ++P+  AP V   G+   ++ FPQ   CIE
Sbjct:   34 IVLGFQHYIVMLGTTVIIPSILVPLMGGGDVEKAEVINTVLFVSGINTLLQSLFGSRLPVVMGASYAYLIPALYITFSYRFTYYLHPHLRFEETMRAIQGALIIASISHMIMGFFGLWRILVRFLSPLSAAPLVILTGVGLLAFAFPQLARCIE 187          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT6_ARATH (Nucleobase-ascorbate transporter 6 OS=Arabidopsis thaliana GN=NAT6 PE=2 SV=2)

HSP 1 Score: 139.043 bits (349), Expect = 9.513e-33
Identity = 107/325 (32.92%), Postives = 148/325 (45.54%), Query Frame = 1

Query: 2485 GRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHT 3459
            G++V  R   V  +V I WIYA  LT GGAY+  G +P                 T   CR D +     A W+R+P+P QWG P F    +  M++ S VA V+S   +                                               V  Y SA++L       P ++SRGI  +G   L++G++G+G GS+   EN   + +T++ SRR +   A F+I FS++GK GA+ ASIP  + AA+ C  +A V A GLS LQ+    SFR   I+G S+FLGLSIP YF +Y               A    GPVHT
Sbjct:  216 GKNVFDR-FAVIFAVVIVWIYAHLLTVGGAYN--GAAPT----------------TQTSCRTDRAGIIGAAPWIRVPWPFQWGAPSFDAGEAFAMMMASFVALVESTGAFVA---------------------------------------------VSRYASATML------PPSILSRGIGWQGVAILISGLFGTGAGSSVSVENAGLLALTRVGSRRVVQIAAGFMIFFSILGKFGAVFASIPAPIIAALYCLFFAYVGAGGLSFLQFCNLNSFRTKFILGFSVFLGLSIPQYFNEYT--------------AIKGYGPVHT 456          

HSP 2 Score: 118.627 bits (296), Expect = 3.584e-26
Identity = 64/158 (40.51%), Postives = 98/158 (62.03%), Query Frame = 3

Query: 1113 IYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTE--HKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            I  G QH+L + G+ + IP+ +VP MGG  ++ A VI T+L V+GI+T+L + FG+RLP V G+S+ ++   + II +  + + +    +F  IMR  QGA+IV S  Q ILGFSGL   ++R ++P+   P V  VG   + +GFP    CIE+ +P
Sbjct:   40 ILLGFQHYLVMLGTTVLIPTALVPQMGGGYEEKAKVIQTILFVAGINTLLQTLFGTRLPAVVGASYTFVPTTISIILSGRFSDTSNPIDRFERIMRATQGALIVASTLQMILGFSGLWRNVVRFLSPISAVPLVGLVGFGLYEFGFPGVAKCIEIGLP 197          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT3_ARATH (Nucleobase-ascorbate transporter 3 OS=Arabidopsis thaliana GN=NAT3 PE=2 SV=2)

HSP 1 Score: 128.642 bits (322), Expect = 2.188e-29
Identity = 95/283 (33.57%), Postives = 129/283 (45.58%), Query Frame = 1

Query: 2527 VTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYF 3375
            + I W +A  LT  GAY+    +                  T + CR D +    +A W+RIPYP QWG PIF       M   ++VAS +S                                                    G + +AS L  +  P   VVSR I L+G   LL GI+GS TG+T   EN   + +T++ SRR +     F+I FS+ GK GA  ASIPL + A + C +  +VVAVG+S +Q+T T S RN+ ++GVSLFL LSI  YF
Sbjct:  247 LAIIWAFAAILTVSGAYNNVSTA------------------TKQSCRTDRAFLMSSAPWIRIPYPFQWGTPIFKASHVFGMFGAAIVASAEST---------------------------------------------------GVFFAASRLAGATAPPAHVVSRSIGLQGIGVLLEGIFGSITGNTASVENVGLLGLTRIGSRRVVQVSTFFMIFFSIFGKFGAFFASIPLPIFAGVYCILLGIVVAVGISFIQFTDTNSMRNMYVIGVSLFLSLSIAQYF 460          

HSP 2 Score: 110.153 bits (274), Expect = 1.997e-23
Identity = 65/174 (37.36%), Postives = 100/174 (57.47%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRN----LTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            L+  +  NP     +    QH++ + G+ + I + +V  MGG   D A VI T+L +SGI+T+L +  G+RLP V G SF Y+ P L II  RDY N      + +FRH MR +QG++I+ S    I+G+      L+R+ +P++V P V+ V L  F  GFP   +C+E+ +P
Sbjct:   44 LQYCIHSNPSWHETVVLAFQHYIVMLGTTVLIANTLVSPMGGDPGDKARVIQTILFMSGINTLLQTLIGTRLPTVMGVSFAYVLPVLSII--RDYNNGQFDSEKQRFRHTMRTVQGSLIISSFVNIIIGYGQAWGNLIRIFSPIIVVPVVSVVSLGLFLRGFPLLANCVEIGLP 215          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT8_ARATH (Nucleobase-ascorbate transporter 8 OS=Arabidopsis thaliana GN=NAT8 PE=2 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 3.459e-29
Identity = 94/289 (32.53%), Postives = 135/289 (46.71%), Query Frame = 1

Query: 2521 LSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWG-VPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQY 3384
             SV I W+YA+ LT GGAY           SN  ++       T   CR D +     + W+R+P+P+QWG  P F+      M+  S V                                                   ++ +  GTY + S   S+ P  P V+SRGI  +GF  LL G++G+G  ++   EN   + +T++ SRR +   A F+I FS++GK GAI ASIP  + AA+ C  ++ V A GLS +Q+    SFR   I+G S+F+GLSIP YF QY
Sbjct:  233 FSVVIVWLYAYILTIGGAY-----------SNTEIN-------TQISCRTDRAGIISASPWIRVPHPIQWGGAPTFNAGDIFAMMAASFV---------------------------------------------------SLVESTGTYIAVSRYASATPIPPSVLSRGIGWQGFGILLCGLFGAGNATSVSVENAGLLAVTRVGSRRVIQVAAGFMIFFSILGKFGAIFASIPAPIVAALYCLFFSYVGAGGLSLIQFCNLNSFRTKFILGFSIFMGLSIPQYFYQY 452          

HSP 2 Score: 112.079 bits (279), Expect = 4.819e-24
Identity = 60/168 (35.71%), Postives = 97/168 (57.74%), Query Frame = 3

Query: 1083 LRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLT--EHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            L   P     I  G QH+L + G+ + IP+++V  +   ++D   +I T+L VSGI+T+  S+FG+RLP V G+S+ Y+   + I+ A  Y ++   + +F  IMR +QGA+I+ S    ++GFSGL   + R ++P+   P VA  G   +  GFP    CIE+ +P
Sbjct:   34 LTSPPPWPETILLGFQHYLVMLGTTVLIPTMLVSKIDARNEDKVKLIQTLLFVSGINTLFQSFFGTRLPAVIGASYSYVPTTMSIVLAARYNDIMDPQKRFEQIMRGIQGALIIASFLHILVGFSGLWRNVTRFLSPLSAVPLVAFSGFGLYEQGFPMLAKCIEIGLP 201          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: V7CLW7_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G146700g PE=4 SV=1)

HSP 1 Score: 402.134 bits (1032), Expect = 2.925e-119
Identity = 215/344 (62.50%), Postives = 252/344 (73.26%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            ++ +L+L GI   GRH + R   VPLS+TI WIYA FLTAGGAY+YKGC+P IPSSNIL+D CRKHAYTM+HCR D SNA  TAAWVRIPYPLQWG+PIFHFRTS+IM+IV+LVASVDS                                                   VGTY + SL V+S+PPTPGVVSRGIALEGFCS+LAG+WGSGTGSTTLTEN HTI+ITK+ASR+ +V GA FLILFS +GKVGA+LASIP +LAA++LCFMWAL  A+GLS L+Y+Q+ SFRNITIVGVSLFLG+SIP YFQQYQ ES L+LPSY VPYAAAS GP  +  +Q+ 
Sbjct:  371 LIFTLYLRGISIFGRH-LFRIYAVPLSLTIIWIYASFLTAGGAYNYKGCNPNIPSSNILLDACRKHAYTMQHCRTDVSNALATAAWVRIPYPLQWGIPIFHFRTSIIMVIVALVASVDS---------------------------------------------------VGTYRATSLQVNSRPPTPGVVSRGIALEGFCSILAGLWGSGTGSTTLTENMHTIDITKVASRKVVVVGATFLILFSFIGKVGALLASIPQALAASVLCFMWALTAALGLSNLRYSQSTSFRNITIVGVSLFLGMSIPAYFQQYQAESSLILPSYLVPYAAASSGPFRSGIKQLD 662          

HSP 2 Score: 301.597 bits (771), Expect = 7.174e-84
Identity = 168/208 (80.77%), Postives = 189/208 (90.87%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV-RTLTYHLVY 1691
            L+ GL+DNPG+  LIYYGLQH+LSL GSL+ IP VMVP MGGTDKDTASVISTML +SGI+TILHSYFG+RLPLVQGSSF+YLAPALVIINA++YRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMS+LLRLINP+VVAPTVAAVGLAFFSYGFPQAG+C E+++P + L+LI TLYLRGISIFG  LFRIYAV  +LT   +Y
Sbjct:  196 LECGLKDNPGIVPLIYYGLQHYLSLTGSLVLIPLVMVPVMGGTDKDTASVISTMLFLSGITTILHSYFGTRLPLVQGSSFVYLAPALVIINAQEYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSVLLRLINPIVVAPTVAAVGLAFFSYGFPQAGTCPEITIPQIALILIFTLYLRGISIFGRHLFRIYAVPLSLTIIWIY 403          

HSP 3 Score: 102.449 bits (254), Expect = 9.368e-19
Identity = 62/118 (52.54%), Postives = 77/118 (65.25%), Query Frame = 3

Query:  450 METGS-NSDSLDK---AETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIE 791
            METGS NS+SL++        G G K G +  K+EPFV + +  HNPRELRSWAKRTGFVS  SGE GT+ S  +F++           +++ GGSSPKIEIDPV+GRTR N   EIE
Sbjct:    1 METGSLNSESLNRNVAMSVRGGTGKKQGGVAGKVEPFVAQSE--HNPRELRSWAKRTGFVSDYSGEAGTSGSA-KFEAF----------ERKGGGSSPKIEIDPVVGRTRRN---EIE 102          

HSP 4 Score: 76.2554 bits (186), Expect = 1.316e-10
Identity = 42/61 (68.85%), Postives = 55/61 (90.16%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            QLDF+INAL+S++MV+TLLVAF+LDNTVPG ++ERGV++WS AED++TDPSL  +YSL  K
Sbjct:  660 QLDFAINALMSLNMVITLLVAFLLDNTVPGRQEERGVFVWSRAEDISTDPSLLSEYSLSKK 720          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: I1J505_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=2)

HSP 1 Score: 401.749 bits (1031), Expect = 3.053e-119
Identity = 216/345 (62.61%), Postives = 255/345 (73.91%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPY-AAASDGPVHTINEQVS 3477
            ++ +L+L GI   GRH + R   VPLS+TI WIYA FLTAGGAY+YKGC+P+IPSSNIL+D CRKHAYTMKHCR D SNA  TAAWVR+PYPLQWG+PIFHFRTS+IM+IVSLVASVDS                                                   VGTY + SL V+S+PPTPGVVSRGIALEGFCS+LAG+WGSGTG+TTLTEN HTI++TK+ASR+ +V GA FLI+FS +GKVGA+LASIPL+LAA++LCFMWAL  A+GLS LQY+Q+ASFRNITIVGVSLFLG+SIP YFQQYQ ES L+LPSY VPY AAAS GP  +  +Q+ 
Sbjct:  363 LIFTLYLRGISIFGRH-LFRIYAVPLSLTIIWIYASFLTAGGAYNYKGCNPDIPSSNILLDACRKHAYTMKHCRTDVSNALSTAAWVRMPYPLQWGIPIFHFRTSIIMVIVSLVASVDS---------------------------------------------------VGTYRATSLQVNSRPPTPGVVSRGIALEGFCSILAGLWGSGTGATTLTENMHTIDVTKVASRKVVVVGAAFLIMFSFIGKVGALLASIPLALAASVLCFMWALTAALGLSNLQYSQSASFRNITIVGVSLFLGMSIPAYFQQYQAESSLVLPSYLVPYAAAASSGPFRSGIKQLD 655          

HSP 2 Score: 299.671 bits (766), Expect = 3.022e-83
Identity = 165/208 (79.33%), Postives = 189/208 (90.87%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV-RTLTYHLVY 1691
            LK GL++NPG+ +LIYYGLQH+LSLAGSL+ IP VM+P MGGTDKDTA+VISTML +SGI+TILHSY G+RLPLVQGSSF+YLAPALVIINA++YRNLTEHKFRHIMRELQGAIIVGS+FQ ILGFSGLMS+LLRLINP+VVAPTVAAVGLAFFSYGFPQAG+C E+++P + LVLI TLYLRGISIFG  LFRIYAV  +LT   +Y
Sbjct:  188 LKCGLKENPGIVSLIYYGLQHYLSLAGSLVLIPLVMIPVMGGTDKDTATVISTMLFLSGITTILHSYLGTRLPLVQGSSFVYLAPALVIINAQEYRNLTEHKFRHIMRELQGAIIVGSVFQCILGFSGLMSILLRLINPIVVAPTVAAVGLAFFSYGFPQAGTCPEITIPQIALVLIFTLYLRGISIFGRHLFRIYAVPLSLTIIWIY 395          

HSP 3 Score: 96.6709 bits (239), Expect = 6.534e-17
Identity = 55/113 (48.67%), Postives = 72/113 (63.72%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRG-----NKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPN 773
            METGS S+SL++    + RG      + G +  K++ FVP+ +  HNPRELRSWAKRTGFVS  SGE GT+ S  +F++           ++R  GSSPKIEIDPV+GR R N
Sbjct:    1 METGSKSESLNRNVAMSVRGGTGKKKQQGGVAGKVDSFVPRSE--HNPRELRSWAKRTGFVSDYSGEAGTSGSA-KFEA----------LERRREGSSPKIEIDPVVGRARQN 100          

HSP 4 Score: 83.1889 bits (204), Expect = 8.928e-13
Identity = 47/61 (77.05%), Postives = 56/61 (91.80%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            QLDF+INAL+S++MVVTLLVAF+LDNTVPGS++ERGVY WS AED+ATDPS Q +YSLP K
Sbjct:  653 QLDFAINALMSLNMVVTLLVAFLLDNTVPGSQEERGVYQWSRAEDIATDPSQQSEYSLPKK 713          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A0B2PBL2_GLYSO (Nucleobase-ascorbate transporter 11 OS=Glycine soja GN=glysoja_024285 PE=4 SV=1)

HSP 1 Score: 401.749 bits (1031), Expect = 3.515e-119
Identity = 216/345 (62.61%), Postives = 255/345 (73.91%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPY-AAASDGPVHTINEQVS 3477
            ++ +L+L GI   GRH + R   VPLS+TI WIYA FLTAGGAY+YKGC+P+IPSSNIL+D CRKHAYTMKHCR D SNA  TAAWVR+PYPLQWG+PIFHFRTS+IM+IVSLVASVDS                                                   VGTY + SL V+S+PPTPGVVSRGIALEGFCS+LAG+WGSGTG+TTLTEN HTI++TK+ASR+ +V GA FLI+FS +GKVGA+LASIPL+LAA++LCFMWAL  A+GLS LQY+Q+ASFRNITIVGVSLFLG+SIP YFQQYQ ES L+LPSY VPY AAAS GP  +  +Q+ 
Sbjct:  363 LIFTLYLRGISIFGRH-LFRIYAVPLSLTIIWIYASFLTAGGAYNYKGCNPDIPSSNILLDACRKHAYTMKHCRTDVSNALSTAAWVRMPYPLQWGIPIFHFRTSIIMVIVSLVASVDS---------------------------------------------------VGTYRATSLQVNSRPPTPGVVSRGIALEGFCSILAGLWGSGTGATTLTENMHTIDVTKVASRKVVVVGAAFLIMFSFIGKVGALLASIPLALAASVLCFMWALTAALGLSNLQYSQSASFRNITIVGVSLFLGMSIPAYFQQYQAESSLVLPSYLVPYAAAASSGPFRSGIKQLD 655          

HSP 2 Score: 299.286 bits (765), Expect = 3.739e-83
Identity = 165/208 (79.33%), Postives = 189/208 (90.87%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV-RTLTYHLVY 1691
            LK GL++NPG+ +LIYYGLQH+LSLAGSL+ IP VM+P MGGTDKDTA+VISTML +SGI+TILHSY G+RLPLVQGSSF+YLAPALVIINA++YRNLTEHKFRHIMRELQGAIIVGS+FQ ILGFSGLMS+LLRLINP+VVAPTVAAVGLAFFSYGFPQAG+C E+++P + LVLI TLYLRGISIFG  LFRIYAV  +LT   +Y
Sbjct:  188 LKCGLKENPGIVSLIYYGLQHYLSLAGSLVLIPLVMIPVMGGTDKDTATVISTMLFLSGITTILHSYLGTRLPLVQGSSFVYLAPALVIINAQEYRNLTEHKFRHIMRELQGAIIVGSVFQCILGFSGLMSILLRLINPIVVAPTVAAVGLAFFSYGFPQAGTCPEITIPQIALVLIFTLYLRGISIFGRHLFRIYAVPLSLTIIWIY 395          

HSP 3 Score: 98.5969 bits (244), Expect = 1.653e-17
Identity = 56/113 (49.56%), Postives = 73/113 (64.60%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRG-----NKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPN 773
            METGS S+SL++    + RG      + G +  K++ FVP+ +  HNPRELRSWAKRTGFVS  SGE GT+ S  +F++           ++R  GSSPKIEIDPV+GRTR N
Sbjct:    1 METGSKSESLNRNVAMSVRGGTGKKKQQGGIAGKVDSFVPRSE--HNPRELRSWAKRTGFVSDYSGEAGTSGSA-KFEA----------LERRREGSSPKIEIDPVVGRTRQN 100          

HSP 4 Score: 83.1889 bits (204), Expect = 9.161e-13
Identity = 47/61 (77.05%), Postives = 56/61 (91.80%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            QLDF+INAL+S++MVVTLLVAF+LDNTVPGS++ERGVY WS AED+ATDPS Q +YSLP K
Sbjct:  653 QLDFAINALMSLNMVVTLLVAFLLDNTVPGSQEERGVYQWSRAEDIATDPSQQSEYSLPKK 713          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A0A0L5J1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G114490 PE=4 SV=1)

HSP 1 Score: 402.905 bits (1034), Expect = 4.395e-119
Identity = 213/357 (59.66%), Postives = 260/357 (72.83%), Query Frame = 1

Query: 2401 LPQFILLNNFGLSRKMVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQ 3471
            +P  +LL        ++ +L+L G+     H + R   VPLSV I W YAFFLTAGGAY++ GCSP+IPSSNILVD CR+HAYTMKHCR D S+AWRTAAWVRIPYPLQWGVPIFH +TS+IMI+VSLV+SVDS+                                                   GTYH+ +L V++KPPTPG+VSRGIA+EGFCS+LAG+WG+G GSTTLTEN HTI++TK+A+RRAL  GA+FLI  S++GKVGA+LASIPL+LAA++LCF WAL+VA+GLSTLQY+QTAS RN+TIVGVSLFLGLSIP YFQQ+Q E+ L+LPSY VPYAAAS+GP HT N+Q
Sbjct:  402 VPHIVLL--------LIFTLYLRGVSIFS-HRVFRIYAVPLSVVIIWAYAFFLTAGGAYNFTGCSPDIPSSNILVDACRRHAYTMKHCRTDVSSAWRTAAWVRIPYPLQWGVPIFHIKTSIIMIMVSLVSSVDSI---------------------------------------------------GTYHTVALRVAAKPPTPGIVSRGIAVEGFCSILAGLWGTGAGSTTLTENVHTIHVTKVANRRALEVGAVFLIFISLIGKVGAVLASIPLALAASVLCFTWALMVALGLSTLQYSQTASIRNMTIVGVSLFLGLSIPAYFQQFQSETSLILPSYLVPYAAASNGPTHTGNKQ 698          

HSP 2 Score: 291.582 bits (745), Expect = 4.223e-80
Identity = 159/198 (80.30%), Postives = 184/198 (92.93%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV 1664
            LK+G  D PG   LIYYGLQ +LSL GS++F+P ++VPAMGGTDKDTA+VIST+LLVSGI+TILHSYFG+RLPLVQGSSF+YLAPAL+I+NA++YRNLTEHKF+HIMRELQGAIIV SIFQSILGFSGLMSL LRLINP+VVAPTVAAVGLAFFSYGFPQAGSC+E+SVP ++L+LI TLYLRG+SIF HR+FRIYAV
Sbjct:  234 LKLGPTDYPGYVPLIYYGLQQYLSLVGSVVFMPLIIVPAMGGTDKDTATVISTLLLVSGITTILHSYFGTRLPLVQGSSFVYLAPALIIMNAQEYRNLTEHKFQHIMRELQGAIIVSSIFQSILGFSGLMSLFLRLINPLVVAPTVAAVGLAFFSYGFPQAGSCVEISVPHIVLLLIFTLYLRGVSIFSHRVFRIYAV 431          

HSP 3 Score: 114.005 bits (284), Expect = 2.619e-22
Identity = 66/117 (56.41%), Postives = 85/117 (72.65%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRGNKLGSMLPK---IEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIE 791
            M+TGS +  ++K ET   RG KLGS+LP    ++ FVP+ D  HNPRELRSWA+RTGFVS  SGE  T+S  E+ + ST F + K G ++R GGSSPKIEIDP+LG+TRPN  +E+E
Sbjct:    1 MDTGSRA--MNKVETGK-RGGKLGSILPNDSTVDRFVPRTD--HNPRELRSWARRTGFVSTFSGE-ATSSVGEK-NESTRFDLEK-GLERRGGGSSPKIEIDPILGQTRPNREIEVE 109          

HSP 4 Score: 74.7146 bits (182), Expect = 3.666e-10
Identity = 42/62 (67.74%), Postives = 49/62 (79.03%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGKF 3846
            Q DF  NAL+S++MVVT L+A VL+NTVPGSR ERGVYIWS AED+  DPSL   YSLP +F
Sbjct:  698 QFDFVFNALMSLNMVVTFLIAIVLENTVPGSRQERGVYIWSHAEDIKNDPSLVATYSLPKRF 759          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A072TRH3_MEDTR (Nucleobase-ascorbate transporter-like protein OS=Medicago truncatula GN=MTR_8g068550 PE=4 SV=1)

HSP 1 Score: 401.749 bits (1031), Expect = 1.037e-118
Identity = 216/359 (60.17%), Postives = 256/359 (71.31%), Query Frame = 1

Query: 2401 LPQFILLNNFGLSRKMVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            +PQ +L+        ++ +L L GI   G H + R   VPLS+TI WIYA FLTAGGAY+YKGC+P IPSSNIL+D C+KHAYTMKHCR D SNA  T++W+RIPYPLQWG PIFHFRT +IM+I SLVASVDS                                                   VGTYHS+SL ++ +PPTPGVVSRGIALEGFCS+LAG+WGSGTGSTTLTEN HTI+ITK+ASRR +  GA F+ILFS +GKVGA+LASIP +LAA++LCFMWAL+ A+GLSTLQY Q+ SFRNITIVGVSLFLGLSIP YFQQYQP+S L+LPSY VPYAAAS GP H+  +Q+ 
Sbjct:  394 IPQIVLV--------LIFTLHLRGISIFGNH-LFRIYAVPLSLTIIWIYASFLTAGGAYNYKGCNPNIPSSNILIDACKKHAYTMKHCRTDISNALSTSSWLRIPYPLQWGFPIFHFRTCIIMVITSLVASVDS---------------------------------------------------VGTYHSSSLRINLRPPTPGVVSRGIALEGFCSILAGLWGSGTGSTTLTENVHTIDITKVASRRVVELGAAFMILFSFMGKVGALLASIPQALAASVLCFMWALITALGLSTLQYGQSGSFRNITIVGVSLFLGLSIPAYFQQYQPQSSLILPSYLVPYAAASSGPFHSGIKQLD 692          

HSP 2 Score: 286.574 bits (732), Expect = 2.055e-78
Identity = 159/208 (76.44%), Postives = 184/208 (88.46%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV-RTLTYHLVY 1691
            +K GL +NPGL  L+YY LQH+LSL GSL+ IP VMVP MGGTD DTA+VISTML +SGI+TILHSYFG+RLPLVQGSSF+YLAPALVI+N+ ++RNLT HKFRHIMRELQGAIIVGS FQ ILGFSGLMSLLLR+INP VVAPTVAAVGLAFFSYGFPQAG+C+E+S+P ++LVLI TL+LRGISIFG+ LFRIYAV  +LT   +Y
Sbjct:  226 MKFGLTENPGLVPLVYYSLQHYLSLIGSLVLIPLVMVPTMGGTDNDTANVISTMLFLSGITTILHSYFGTRLPLVQGSSFVYLAPALVIMNSEEFRNLTHHKFRHIMRELQGAIIVGSTFQCILGFSGLMSLLLRIINPTVVAPTVAAVGLAFFSYGFPQAGTCMEISIPQIVLVLIFTLHLRGISIFGNHLFRIYAVPLSLTIIWIY 433          

HSP 3 Score: 97.4413 bits (241), Expect = 3.658e-17
Identity = 63/118 (53.39%), Postives = 74/118 (62.71%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRGNK--LGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMD 797
            METGS+ D  DK +   G  NK    S   KIEPFVP++D  HNP+EL++WAK+TGFVS  S E   ASS+   DS     V     D R GG+SPKIEIDPVLG  RPN   EIE+D
Sbjct:    1 METGSSLDIFDKGKVMRGVRNKKKQDSNAIKIEPFVPRRD--HNPKELKTWAKKTGFVSDYSSEA-GASSSANLDS-----VIVVDDDHREGGNSPKIEIDPVLGIARPNRDDEIELD 110          

HSP 4 Score: 68.9366 bits (167), Expect = 2.624e-8
Identity = 40/61 (65.57%), Postives = 50/61 (81.97%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            QLDF+INAL+S++MVVTL VAF+L+NTVP S  ER VYIW+  +D+ATDPSL   YS+P K
Sbjct:  690 QLDFAINALMSLNMVVTLFVAFILENTVPRSSQERAVYIWTRPQDIATDPSLASAYSMPRK 750