g.barbadense_cottongen_reftransV1_0094177, g.barbadense_cottongen_reftransV1_0094177 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy Swiss-Prot
Match: CALS9_ARATH (Callose synthase 9 OS=Arabidopsis thaliana GN=CALS9 PE=2 SV=2)

HSP 1 Score: 2922.11 bits (7574), Expect = 0.000e+0
Identity = 1466/1890 (77.57%), Postives = 1662/1890 (87.94%), Query Frame = -2

Query:  224 GSVGHAAGGIAGYVPSSL-NNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDVARLLEFYRLYREKNNVDKLREEEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQLTEEIPVELKRVIDSDAAMTEDLIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSIPETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQPVWSNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIAQPANSC-------SKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWR-KTSFFQKPEPRSKNPLKLGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFNDGHLNA-KTLREVLSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIASVFVSFLYVRALQEESKPNSNSVVFRXXXXXXXXXXXIHFFISFLMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLH-PV--RXXXXXXXXXXXXXKFDAARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGKLPLVQWPLFLLASKIFLAKDIAAESRDSQDELWERISRDEYMKYAVQECYYALRYILTAILEAEGRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGILNQAEKPEHEKGAVNAVQDLYDVVRHDVLAIYLREHSDQWQSILKARTEGRLFAKLNWPRDPELKAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEAALSRLETTDTQGYELSPEARARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFIDVVETLKDGKVHTEYYSKLVKADINGKDKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYGYNDX-XXXXXXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIRTMRGRILETILSLRFFLFQYGVVYKLNVQGTNTSLTVYGFSWVVLAVLIILFKVFTFSQKMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGNNPNTGL 5851
            G  G     I GYVPSSL NNRDID ILR ADEIQDE+PN+ARILCEH YSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQD+ RL EFYRLYREKNNVD L+EEE  LRESG F+    ELERKT+KRKRVF TL+VLG VLEQL +EIP ELK VIDSDAAM+ED IAYNIIP  LDAP  TNA  +FPEV+AAV+ALK++  LPKLP DF IP TR+ D++DFLHY+FGFQKD+VSNQREH+VLLLANEQSR  IPEE EPKLD+AAV+KVFLKSL+NYIKWC+YLCIQP WSNL+A+N +KK+LF+SLYFLIWGEAANIRFLPECLCYIFHHM REMDE LRQ +A+PA SC       S DGV SFLD VI PLY VV+AEA NN+NGRAPHSAWRNYDDFNEYFWSLH F+L WPWR  +SFFQKP PR K  LK G  +HRGKTSFVEHRTF HLYHSFHRLWIFL MMFQ L IIAFN   L + KTL ++LSLGPTFVVMKF+ESVL+VIMMYGAYSTTRRLAVSRIFLRFIWF +ASVF+SFLYV++L+    PNS+S + +LYLIVI IYGG+ FF S LMRIP CH +   CD   +IRF KWMRQER+YVGRGMYERT+DFIKY++FWL++LS KF+FAYF QIKPLV PTR ++  +NI YSWHDFVSR N+NALTV SLWAPV+A+YLLDI+IFYT+ SA  GFLLGARDRLGEIRSL+A+ KLFEEFP AFM+ LH P+  R S +S   V +K+K DAA F+PFWN+IIK+LREEDY+T+FEMELL MPKN+G+L LVQWPLFLL+SKI LAK+IAAES +SQ+E+ ERI RD+YMKYAV+E Y+ L+ +LT  LEAEGR WVERIYE I+ S+ ++ I  DFQLNKL LVI+RVTALLGIL + E PEH KGA+ A+QDLYDV+R D+L   +R H + W  + +A  EGRLF KL WP+DPELKA VKRLYSL TIKDSA++VP+NLEARRRL+FFTNSLFMD+PP + V++MLSFSVFTPYYSE+VLYSM EL K+NEDGISILFYLQKIYPDEWKNFLARIGRDENA E +L D+  DILELRFWASYRGQTLARTVRGMMYYRKALMLQ+YLER+   D          TD +G+ELSPEARA+ADLKFTYVVTCQIYGRQKE+QKPEA DIALLMQRNEALR+A+IDVV++ K+GK HTEYYSKLVKADI+GKDKEIY+IKLPGDPKLGEGKPENQNHAIVFTRGNA+QTIDMNQDNYFEEALK+RNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQE+SFVTLGQRVLA PLK+RMHYGHPDVFDRVFHITRGGISKASR+INISEDI+AGFN+TLRQGN+THHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQL DFFRMMSF+FTTVGFY CTMLTVLT+YIFLYGRAYLALSGVG T++ RA + D+ AL AALN QFLFQIG+F+AVPMVLGFILEQGFL+AIVSFITMQ QLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVV+HIKFSENYRLYSRSHFVK +EV+LLLVVYLAYG +++ A+SYILL++SSWF+A+SWLFAPYLFNP+GFEWQK+VEDF++WTNWLFYRGGIGVKG ESWEAWW+EE++HIRT+ GRI+ETILSLRFF+FQYG+VYKL +QG++TS  VYG+SWV  A++I+LFKVFTFSQK+SVNFQLLLRFIQG+S ++A+AG+ VAV LT LS+ DIFA +LAF+PTGWGILSIA AWKP +K+ G+WKS+RS+ARLYDA MGM+IF+PVA  SWFPFVSTFQTR+MFNQAFSRGLEISLILAG+NPN+GL
Sbjct:   23 GVAGGNQSSIVGYVPSSLSNNRDIDAILRAADEIQDEDPNIARILCEHGYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDILRLQEFYRLYREKNNVDTLKEEEKQLRESGAFTD---ELERKTVKRKRVFATLKVLGSVLEQLAKEIPEELKHVIDSDAAMSEDTIAYNIIP--LDAPVTTNATTTFPEVQAAVAALKYFPGLPKLPPDFPIPATRTADMLDFLHYIFGFQKDSVSNQREHIVLLLANEQSRLNIPEETEPKLDDAAVRKVFLKSLENYIKWCDYLCIQPAWSNLEAINGDKKLLFLSLYFLIWGEAANIRFLPECLCYIFHHMVREMDEILRQQVARPAESCMPVDSRGSDDGV-SFLDHVIAPLYGVVSAEAFNNDNGRAPHSAWRNYDDFNEYFWSLHSFELGWPWRTSSSFFQKPIPRKK--LKTGRAKHRGKTSFVEHRTFLHLYHSFHRLWIFLAMMFQALAIIAFNKDDLTSRKTLLQILSLGPTFVVMKFSESVLEVIMMYGAYSTTRRLAVSRIFLRFIWFGLASVFISFLYVKSLK---APNSDSPIVQLYLIVIAIYGGVQFFFSILMRIPTCHNIANKCDRWPVIRFFKWMRQERHYVGRGMYERTSDFIKYLLFWLVVLSAKFSFAYFLQIKPLVGPTRMIVKQNNIPYSWHDFVSRKNYNALTVASLWAPVVAIYLLDIHIFYTIFSAFLGFLLGARDRLGEIRSLEAIHKLFEEFPGAFMRALHVPLTNRTSDTSHQTVDKKNKVDAAHFAPFWNQIIKSLREEDYITDFEMELLLMPKNSGRLELVQWPLFLLSSKILLAKEIAAES-NSQEEILERIERDDYMKYAVEEVYHTLKLVLTETLEAEGRLWVERIYEDIQTSLKERNIHHDFQLNKLSLVITRVTALLGILKENETPEHAKGAIKALQDLYDVMRLDILTFNMRGHYETWNLLTQAWNEGRLFTKLKWPKDPELKALVKRLYSLFTIKDSAAHVPRNLEARRRLQFFTNSLFMDVPPPKSVRKMLSFSVFTPYYSEVVLYSMAELTKRNEDGISILFYLQKIYPDEWKNFLARIGRDENALEGDL-DNERDILELRFWASYRGQTLARTVRGMMYYRKALMLQSYLERKAGNDA---------TDAEGFELSPEARAQADLKFTYVVTCQIYGRQKEDQKPEAVDIALLMQRNEALRIAYIDVVDSPKEGKSHTEYYSKLVKADISGKDKEIYSIKLPGDPKLGEGKPENQNHAIVFTRGNAIQTIDMNQDNYFEEALKMRNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLAKPLKIRMHYGHPDVFDRVFHITRGGISKASRVINISEDIFAGFNTTLRQGNVTHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLLDFFRMMSFFFTTVGFYLCTMLTVLTVYIFLYGRAYLALSGVGATIRERAILLDDTALSAALNAQFLFQIGVFTAVPMVLGFILEQGFLQAIVSFITMQFQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVKHIKFSENYRLYSRSHFVKAMEVILLLVVYLAYGNDEAGAVSYILLTVSSWFLAVSWLFAPYLFNPAGFEWQKVVEDFKEWTNWLFYRGGIGVKGAESWEAWWEEELSHIRTLSGRIMETILSLRFFIFQYGIVYKLKLQGSDTSFAVYGWSWVAFAMIIVLFKVFTFSQKISVNFQLLLRFIQGLSLLMALAGIIVAVVLTPLSVTDIFACVLAFIPTGWGILSIACAWKPVLKRMGMWKSIRSLARLYDALMGMLIFLPVALCSWFPFVSTFQTRMMFNQAFSRGLEISLILAGDNPNSGL 1890          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy Swiss-Prot
Match: CALSA_ARATH (Callose synthase 10 OS=Arabidopsis thaliana GN=CALS10 PE=2 SV=5)

HSP 1 Score: 2396.7 bits (6210), Expect = 0.000e+0
Identity = 1192/1897 (62.84%), Postives = 1479/1897 (77.97%), Query Frame = -2

Query:  230 GH--AAGGIAGYVPSSLNN-RDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDVARLLEFYRLYREKNNVDKLREEEMMLRESGV-FSGNLGELERKTLKRKRVFGTLRVLGMVLEQLTEE---------IPVELKRVIDSDAAMTEDLIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSIPETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQPVWSNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIAQPANSC---SKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWRKTS-FFQKPEPRSKNPLKLGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFNDGHLNAKTLREVLSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIASVFVSFLYVRALQEESKPNSNSVVFRXXXXXXXXXXXIHFFISFLMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLHPVRXXXXXXXXXXXXXKFD-----AARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGKLPLVQWPLFLLASKIFLAKDIAAESRDSQDELWERISRDEYMKYAVQECYYALRYILTAILEAEGRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGILNQAEKPEHEKGAVNAVQDLYDVVRHDVLAIYLREHSDQWQSILKARTEGRLFAKLNWPRDPELKAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEAALSRLETTDTQGYELSPEARARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFI---DVVETLKDGKVHTEYYSKLVKADINGKDKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYGYNDXXXXX-XXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIRTMRGRILETILSLRFFLFQYGVVYKLNVQGTNTSLTVYGFSWVVLAVLIILFKVFTFSQKMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGNNPNT 5842
            GH   + G+AG VP SL    +ID IL+ ADEIQ E+P+VARILCE AYS+AQNLDPNS+GRGVLQFKTGLMSVIKQKLAKR+  +IDR +D+ RL EFY+LY+ ++ VD +++EE   RESG  FS N+GE+    LK ++VF TLR L  VLE L+ +         I  EL R+  +DA ++ +L  YNI+P  L+A ++TNAI  FPEVR AV A+++    P+LP DF I   R  D+ D L Y+FGFQ+DNV NQREH+VL L+N QS+  IP + +PK+DE AV +VFLK LDNYIKWC YL I+ V++ L+A+++++K+  VSLYFLIWGEAAN+RFLPEC+CYIFH+MA+E+D  L    A  A+SC   +  G VSFL+++I P+Y+ ++AE   N  G+A HS WRNYDDFNEYFW+  CF+LSWP +  S F  KP+ R +            K+SFVEHRT+ HL+ SF RLWIF+ +MFQ LTIIAF + HLN +T + +LS GPT+ +M F E +LDV++MYGAYS  R +A+SR+ +RF+W+ + S FV + YV+ L E +KPN N   F LY++V+G Y  +      L+++PACH L+E+ D  S  +F KW+ QERY+VGRG++E  +D+ +Y+ FWL++L+ KF FAYF QIKPLVKPT T+I +   +YSWHD VS++N +ALT+VSLWAPV+A+YL+DI+I+YT+LSA+ G ++GA+ RLGEIR+++ V K FE FP+AF + L           +   +   D     AA FSPFWNEIIK+LREEDYL+N EM+LL +P NTG L LVQWPLFLL SKI +A D+A E +++Q+ LW +I  DEYM YAVQECYY++  IL +++  EGR WVERI+  I  SI + +++    L KLQLV+SR TAL G+L + E P+  KGA  A+ D Y+VV HD+L+  LRE  D W  + +AR EGRLF+++ WPRDPE+  QVKRL+ LLT+KD+A+NVPKNLEARRRLEFFTNSLFMDMP ARPV EM+ FSVFTPYYSE VLYS +EL  +NEDGISILFYLQKI+PDEW+NFL RIGR E+  + +L  S +D LELRFW SYRGQTLARTVRGMMYYR+ALMLQ++LER      +A+L+ +     +G+E S EARA+ADLKFTYVV+CQIYG+QK+++KPEA DI LL+QR EALRVAFI   DV           E+YSKLVKADI+GKD+EIY+IKLPGDPKLGEGKPENQNHAIVFTRG A+QTIDMNQDNY EEA+K+RNLLEEF   HGIR PTILGVREHVFTGSVSSLA FMSNQE+SFVTLGQRVLA PLKVRMHYGHPDVFDR+FHITRGGISKASR+INISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYR+GQLFDFFRMMSFYFTTVGFY CTM+TVLT+Y+FLYGR YLA SG    +   A+++ N AL+AALN QFL QIGIF+AVPMV+GFILE G L+AI SFITMQ QLC+VFFTFSLGTRTHYFGRTILHGGA+Y+ATGRGFVV+HIKF++NYRLYSRSHFVK  EV LLL++Y+AYGY D   S  +LL+ISSWF+ +SWLFAPY+FNPSGFEWQK VEDF DW +WL Y+GG+GVKGE SWE+WW+EE AHI+T+RGRILETILSLRFF+FQYG+VYKL++   NTSL +YG+SWVVL V++ LFK+F +S + S N  L LRF+QGV+ +  IA + VA+A+TDLSIPD+FA +L F+PTGW +LS+A  WK  ++  GLW++VR   R+YDA MGM+IF P+A  SWFPF+STFQ+RL+FNQAFSRGLEIS+ILAGN  N 
Sbjct:   27 GHERVSSGLAGAVPPSLGRATNIDAILQAADEIQSEDPSVARILCEQAYSMAQNLDPNSDGRGVLQFKTGLMSVIKQKLAKRDGASIDRDRDIERLWEFYKLYKRRHRVDDIQKEEQKWRESGTTFSSNVGEI----LKMRKVFATLRALIEVLEVLSRDADPNGVGRSIRDELGRIKKADATLSAELTPYNIVP--LEAQSMTNAIGVFPEVRGAVQAIRYTEHFPRLPVDFEISGQRDADMFDLLEYIFGFQRDNVRNQREHLVLTLSNAQSQLSIPGQNDPKIDENAVNEVFLKVLDNYIKWCKYLRIRVVYNKLEAIDRDRKLFLVSLYFLIWGEAANVRFLPECICYIFHNMAKELDAKLDHGEAVRADSCLTGTDTGSVSFLERIICPIYETISAETVRNNGGKAAHSEWRNYDDFNEYFWTPACFELSWPMKTESRFLSKPKGRKRT----------AKSSFVEHRTYLHLFRSFIRLWIFMFIMFQSLTIIAFRNEHLNIETFKILLSAGPTYAIMNFIECLLDVVLMYGAYSMARGMAISRLVIRFLWWGLGSAFVVYYYVKVLDERNKPNQNEFFFHLYILVLGCYAAVRLIFGLLVKLPACHALSEMSDQ-SFFQFFKWIYQERYFVGRGLFENLSDYCRYVAFWLVVLASKFTFAYFLQIKPLVKPTNTIIHLPPFQYSWHDIVSKSNDHALTIVSLWAPVLAIYLMDIHIWYTLLSAIIGGVMGAKARLGEIRTIEMVHKRFESFPEAFAQNLVSPVVKRVPLGQHASQDGQDMNKAYAAMFSPFWNEIIKSLREEDYLSNREMDLLSIPSNTGSLRLVQWPLFLLCSKILVAIDLAMECKETQEVLWRQICDDEYMAYAVQECYYSVEKILNSMVNDEGRRWVERIFLEISNSIEQGSLAITLNLKKLQLVVSRFTALTGLLIRNETPDLAKGAAKAMFDFYEVVTHDLLSHDLREQLDTWNILARARNEGRLFSRIAWPRDPEIIEQVKRLHLLLTVKDAAANVPKNLEARRRLEFFTNSLFMDMPQARPVAEMVPFSVFTPYYSETVLYSSSELRSENEDGISILFYLQKIFPDEWENFLERIGRSESTGDADLQASSTDALELRFWVSYRGQTLARTVRGMMYYRRALMLQSFLERRGLGVDDASLTNM----PRGFESSIEARAQADLKFTYVVSCQIYGQQKQQKKPEATDIGLLLQRYEALRVAFIHSEDVGNGDGGSGGKKEFYSKLVKADIHGKDEEIYSIKLPGDPKLGEGKPENQNHAIVFTRGEAIQTIDMNQDNYLEEAIKMRNLLEEFHGKHGIRRPTILGVREHVFTGSVSSLAWFMSNQETSFVTLGQRVLAYPLKVRMHYGHPDVFDRIFHITRGGISKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRIGQLFDFFRMMSFYFTTVGFYVCTMMTVLTVYVFLYGRVYLAFSGADRAISRVAKLSGNTALDAALNAQFLVQIGIFTAVPMVMGFILELGLLKAIFSFITMQFQLCSVFFTFSLGTRTHYFGRTILHGGAKYRATGRGFVVQHIKFADNYRLYSRSHFVKAFEVALLLIIYIAYGYTDGGASSFVLLTISSWFLVISWLFAPYIFNPSGFEWQKTVEDFEDWVSWLMYKGGVGVKGELSWESWWEEEQAHIQTLRGRILETILSLRFFMFQYGIVYKLDLTRKNTSLALYGYSWVVLVVIVFLFKLFWYSPRKSSNILLALRFLQGVASITFIALIVVAIAMTDLSIPDMFACVLGFIPTGWALLSLAITWKQVLRVLGLWETVREFGRIYDAAMGMLIFSPIALLSWFPFISTFQSRLLFNQAFSRGLEISIILAGNRANV 1902          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy Swiss-Prot
Match: CALS5_ARATH (Callose synthase 5 OS=Arabidopsis thaliana GN=CALS5 PE=1 SV=1)

HSP 1 Score: 1544.25 bits (3997), Expect = 0.000e+0
Identity = 890/1915 (46.48%), Postives = 1210/1915 (63.19%), Query Frame = -2

Query:  239 VPSSLNNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDVARLLEFYRLYREK--NNVDKLRE-EEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQLTEEIPVELKRVIDSDAAMTEDLIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSI--PETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQPVWSNLDAVN--KEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIA-------QPANSCSKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWRKTSFFQKPEPRSKNPLKLGG---GQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFNDGHLNAKTLREVL-SLGPTFVV---MKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIASVFVSFLYVRALQEESKPNSNSVVF----RXXXXXXXXXXXIHFFISFLMRI----PACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLHPVRXX-------XXXXXXXXXXXKFDAARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTG-KLPLVQWPLFLLASKIFLAKDIAAESRDSQDELWERISRDEYMKYAVQECYYALRYILTAILEAEG-RTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGILNQAEKPEHEKGAVNAVQDLYDVVRHDVLAIYLRE-----HSDQWQS-ILKARTEGRLFAKLNWPRDPELKAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEA---ALSRLETTDTQGY-ELSPEARARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFIDVVETLKDGKVHTEYYSKLVKADINGKDKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYG-YNDXXXXXXXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHI--RTMRGRILETILSLRFFLFQYGVVYKLNV-----QGTNTSLTVYGFSWVVLAVLIILFKVFTFS-QKMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGNN 5812
            VP+SL    I  ILRVA EI+ E P VA +   +A+  A  LDP+S GRGV QFKT L   +++  A      + ++ D   +  FY+ Y E     +D+  + +   L ++   +G L E+     K ++V           E +  EI    + V + +    E    YNI+P  LD+   + +++   EV+AAV+AL +   L   PS F     +T + DL+D+L  +FGFQ+DNV NQREH+V L A+   R     EP  KLD+ AV  V  K   NY  WC +L  +       A    +++KIL++ LY LIWGEAANIRF+PECLCYIFH+MA E+   L  +++       +P+     +   +FL +VITP+Y VV  EA  N NG+A HS W NYDD NEYFW+  CF L WP R      K   R     K G        GK++F E RTF+H+YHSF RLW F ++  Q + I+AF    L     ++VL +L   F+    ++F +SVLDVI+ +  +   +   V R  L+ +      V +   Y +++          + F    +    +  +   ++   + L  I    P   R  E  D   + R + W  Q R YVGRGM+E     IKY IFWL++   KFAF+YF Q+K LVKPT  ++++ +++Y WH+F     HN   VVSLW PVI +Y +D  I+Y + S + G ++GA DRLGEIR+L  ++  F+  P AF   L P   +       +   +EV    + +AA+FS  WNEII + REED +++ EM+LL +P  +   L L+QWP FLLASKI +A D+AA+ R    +LW+RI  DEYMK AV ECY + +++L  ++  E  +  +  I + +E++I+K +   +F++  L  + S+   L+GIL  A+ P      V  +QD+ +VV  D++    RE     H+++     L A T+ +           +   Q+ RL+ LLT+K+SA +VP NLEA+RR+ FFTNSLFMDMP A  V+ MLSFSV TPYYSE  +YS N+L  +NEDG+S+++YLQKI+PDEW NFL R+   +   ET + +S  +IL+LR W S RGQTL RTVRGMMYYR+AL LQ +L+  N  +  A   A+S     D +    L  +  A ADLKFTYV TCQ YG QK      A DI  LM  N +LRVA+ID VE  + GKV   +YS L+KA ++  D+EIY IKLPG  K+GEGKPENQNHA++FTRG A+Q IDMNQD+Y EEALK+RNLLEEF+ DHG+R PTILG REH+FTGSVSSLA FMSNQE+SFVT+GQRVLA+PLKVR HYGHPDVFDR+FHITRGGISKASR IN+SEDI+AGFNSTLR+GN+THHEYIQVGKGRDVGLNQI+LFE KVA GNGEQ LSRD+YRLG  FDFFRMMS YFTTVGFY  +M+ VLT+Y FLYGR YL+LSGV E +   A    +++L+AA+ +Q + Q+G+   +PMV+   LE+GF  A+   I MQLQL  VFFTFSLGT+ HY+GRTILHGG++Y+ATGRGFVV+H KF+ENYR+YSRSHFVKG+E+++LL+ Y  YG   + ++ Y L+  S+WF+  SWLFAP+ FNPSGFEWQKIV+D+ DW  W+  RGGIGV   +SWE+WW+EE  H+      G+  E  LSLR+F++QYG+VY+LN+      G   S+ VYG SW+V+  ++I+ K+ +   +K S +FQL+ R ++   F+ ++  V +      L++ DI  S+LAF+PTGW +L I+   +P +K  G+W SV+++AR Y+  MG++IF+PV   +WFPFVS FQTRL+FNQAFSRGL+I  ILAG  
Sbjct:   37 VPASLGT--IAPILRVAAEIEHERPRVAYLCRFYAFEKAHRLDPSSGGRGVRQFKTLLFQRLERDNASSLASRVKKT-DGREVESFYQQYYEHYVRALDQGDQADRAQLGKAYQTAGVLFEVLMAVNKSEKV-----------EAVAPEIIAAARDVQEKN----EIYAPYNILP--LDSAGASQSVMQLEEVKAAVAALGNTRGL-NWPSGFEQHRKKTGNLDLLDWLRAMFGFQRDNVRNQREHLVCLFADNHIRLTPKPEPLNKLDDRAVDTVMSKLFKNYKNWCKFLGRKHSLRLPQAAQDIQQRKILYMGLYLLIWGEAANIRFMPECLCYIFHNMAYELHGLLAGNVSIVTGENIKPSYGGDDE---AFLRKVITPIYRVVQTEANKNANGKAAHSDWSNYDDLNEYFWTPDCFSLGWPMRDDGDLFK-STRDTTQGKKGSFRKAGRTGKSNFTETRTFWHIYHSFDRLWTFYLLALQAMIILAFERVELREILRKDVLYALSSIFITAAFLRFLQSVLDVILNFPGFHRWKFTDVLRNILKIVVSLAWCVVLPLCYAQSVSFAPGKLKQWLSFLPQVKGVPPLYIMAVALYLLPNVLAAIMFIFPMLRRWIENSD-WHIFRLLLWWSQPRIYVGRGMHESQIALIKYTIFWLLLFCCKFAFSYFLQVKLLVKPTNAIMSIRHVKYKWHEFFPNAEHNYGAVVSLWLPVILVYFMDTQIWYAIFSTICGGVIGAFDRLGEIRTLGMLRSRFQSLPGAFNTYLVPSDKTRRRGFSLSKRFAEVTAARRTEAAKFSQLWNEIISSFREEDLISDREMDLLLVPYTSDPSLKLIQWPPFLLASKIPIALDMAAQFRTRDSDLWKRICADEYMKCAVIECYESFKHVLHTLVIGENEKRIIGIIIKEVESNISKNSFLSNFRMAPLPALCSKFVELVGILKNAD-PAKRDTVVLLLQDMLEVVTRDMMQNENRELVELGHTNKESGRQLFAGTDAKPAILFPPVATAQWHEQISRLHLLLTVKESAMDVPTNLEAQRRIAFFTNSLFMDMPRAPRVRNMLSFSVLTPYYSEETVYSKNDLEMENEDGVSVVYYLQKIFPDEWTNFLERL---DCKDETSVLESEENILQLRHWVSLRGQTLFRTVRGMMYYRRALKLQAFLDMANETEILAGYKAISEPTEEDKKSQRSLYTQLEAVADLKFTYVATCQNYGNQKRSGDRRATDILNLMVNNPSLRVAYIDEVEEREGGKVQKVFYSVLIKA-VDNLDQEIYRIKLPGPAKIGEGKPENQNHALIFTRGEALQAIDMNQDHYLEEALKMRNLLEEFNEDHGVRAPTILGFREHIFTGSVSSLAWFMSNQETSFVTIGQRVLASPLKVRFHYGHPDVFDRIFHITRGGISKASRGINLSEDIFAGFNSTLRRGNVTHHEYIQVGKGRDVGLNQISLFEAKVACGNGEQTLSRDLYRLGHRFDFFRMMSCYFTTVGFYISSMIVVLTVYAFLYGRLYLSLSGVEEAIVKFAAAKGDSSLKAAMASQSVVQLGLLMTLPMVMEIGLERGFRTALSDLIIMQLQLAPVFFTFSLGTKVHYYGRTILHGGSKYRATGRGFVVKHEKFAENYRMYSRSHFVKGMELMVLLICYRIYGKAAEDSVGYALVMGSTWFLVGSWLFAPFFFNPSGFEWQKIVDDWDDWNKWISSRGGIGVPANKSWESWWEEEQEHLLHSGFFGKFWEIFLSLRYFIYQYGIVYQLNLTKESRMGKQHSIIVYGLSWLVIVAVMIVLKIVSMGRKKFSADFQLMFRLLKLFLFIGSVVIVGMLFHFLKLTVGDIMQSLLAFLPTGWALLQISQVARPLMKTVGMWGSVKALARGYEYIMGVVIFMPVTVLAWFPFVSEFQTRLLFNQAFSRGLQIQRILAGGK 1920          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy Swiss-Prot
Match: CALS2_ARATH (Callose synthase 2 OS=Arabidopsis thaliana GN=CALS2 PE=2 SV=3)

HSP 1 Score: 1540.01 bits (3986), Expect = 0.000e+0
Identity = 888/1956 (45.40%), Postives = 1221/1956 (62.42%), Query Frame = -2

Query:  233 VPSSLNNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQ--DVARLLEFYRLYREK------NNVDKLREEEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQLTEEIPVEL-KRVIDSDAAMTED---LIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSIPETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQP-VW-SNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIA-------QPANSCSKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWRKTS--FFQKPEP----RSKNPLKLGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFN-----DGHLNAKTLREVLSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIAS-VFVSFLYVRALQEESKPNSNSVVFRXXXXXXXXXXXIHFFI------------SFLMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLHP---VRXXXXXXXXXXXXXKFD---------AARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGK-LPLVQWPLFLLASKIFLAKDIAAESRDSQDELWERISRDEYMKYAVQECYYALRYILTAIL--EAEGRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGILNQAEKPEHEKGAVNAVQDLYDVVRHDVL-----AIYLREHSD---QWQSILKARTEGRLFAKLNWP---RDPELKAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEAALSRLETT----DTQGYELSPEARARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFIDVVE-TLKD---GKVHTEYYSKLVKADINGK-----------DKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDH-GIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYGYN-DXXXXXXXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIR--TMRGRILETILSLRFFLFQYGVVYKLNV-QGTNTSLTVYGFSW-VVLAVLIILFKVFTFSQKMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGNNPN 5812
            VPSSL   +I  ILRVA+E++   P VA +   +A+  A  LDP S GRGV QFKT L+    Q+L +    T+   Q  D   +  FY+ Y +K      N  DK    ++                       + + T  VL  VL+ + +   VE+   ++++   + E     + YNI+P  LD  +   AI+ FPE++A VSAL++   LP  P+     +    D++D+L  +FGFQKDNVSNQREH++LLLAN   R     E +P+LD+ A+  V  K   NY KWC YL  +  +W   +    +++K+L++ LY LIWGEAAN+RFLPECLCYI+HHMA E+   L   ++       +PA     +   +FL +V+TP+Y  +A EA  +  G++ HS WRNYDD NEYFWS+ CF L WP R  +  F Q  E     RS+N  K G  +  GK +FVE R+F+H++ SF R+W F ++  Q + IIA+N      G        +VLS+  T  ++K  ++VLD+ + +     +R      + LRFI+ ++A+ ++V  + +        P+  +   + +           F I            + L   P   R  E  D   ++  + W  Q R Y+GRGM+E      KY +FW+++L  K AF+++ +IKPLVKPT+ ++ +    Y WH+F      N   V++LW+PVI +Y +D  I+Y ++S + G L GA  RLGEIR+L  ++  F+  P+AF   L P                  KFD         AARF+  WN+II + REED +++ EMELL +P    + L L++WP FLLASKI +A D+A +S     EL +R+S D YM  AV+ECY + + ++  ++  E EG+  +  I+  I+  I K+T+  D  L+ L  +  +   L+  L +  + E +   V  + ++ +VV  D++     ++    H+    ++  +     + + F++L +P   +    K ++KRL+ LLT+K+SA +VP NLEARRRL FF+NSLFM+MP A  ++ MLSFSV TPYYSE VL+S+  L K+NEDG+SILFYLQKI+PDEW NFL R+   +  +E EL        ELR WASYRGQTL +TVRGMMYYRKAL LQ +L+     +       LE T       G  L  + +A AD+KFT+VV+CQ Y  QK      A DI  LM    +LRVA+ID VE T K+   G     YYS LVKA    K           D+ IY IKLPG   LGEGKPENQNH+I+FTRG  +QTIDMNQDNY EEA K+RNLL+EF   H G+R PTILG+REH+FTGSVSSLA FMSNQE+SFVT+GQRVLA+PLKVR HYGHPDVFDR+FH+TRGG+ KAS++IN+SEDI+AGFNSTLR+GN+THHEYIQVGKGRDVGLNQI++FE K+A GNGEQ LSRD+YRLG  FDFFRM+S YFTT+GFYF TMLTVLT+Y+FLYGR YL LSG+ E +  +     N  L+AAL +Q   QIG   A+PM++   LE+GF  A++ F+ MQLQL +VFFTF LGT+THY+GRT+ HGGA Y+ TGRGFVV H KF+ENYR YSRSHFVKG+E+++LL+VY  +G+     ++YIL+++S WFM ++WLFAP+LFNPSGFEWQKIV+D+ DW  W++ RGGIGV  E+SWE+WW++E+ H+R    RG ILE +L+LRFF+FQYG+VY+L+  +  N SL +YG SW V+L +L+I+  +    Q+ S NFQLL R I+G  F+  +  +   +AL  L+  DIF  +LAF+PTGWG+L IA A KP +++ G W SVR++AR Y+  MG+++F PVAF +WFPFVS FQTR++FNQAFSRGL+IS IL G   +
Sbjct:   35 VPSSLV--EIAPILRVANEVEASNPRVAYLCRFYAFEKAHRLDPTSSGRGVRQFKTALL----QRLERENETTLAGRQKSDAREMQSFYQHYYKKYIQALQNAADKADRAQL----------------------TKAYQTAAVLFEVLKAVNQTEDVEVADEILEAHTKVEEKSQIYVPYNILP--LDPDSQNQAIMRFPEIQATVSALRNTRGLP-WPAGHK--KKLDEDMLDWLQTMFGFQKDNVSNQREHLILLLANVHIRQFPRPEQQPRLDDRALTIVMKKLFKNYKKWCKYLGRKSSLWLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFLPECLCYIYHHMAFELYGMLAGSVSPMTGEHVKPAYGGEDE---AFLQKVVTPIYKTIAKEAKRSRGGKSKHSEWRNYDDLNEYFWSIRCFRLGWPMRADADFFCQTAEELRLDRSENKPKTGD-RWMGKVNFVEIRSFWHIFRSFDRMWSFYILSLQAMIIIAWNGSGKLSGIFQGDVFLKVLSIFITAAILKLAQAVLDIALSW----KSRHSMSFHVKLRFIFKAVAAAIWVVLMPLTYAYSWKTPSGFAETIKNWFGGHQNSSPSFFIIVILIYLSPNMLSTLLFAFPFIRRYLERSD-YKIVMLMMWWSQPRLYIGRGMHESALSLFKYTMFWVVLLISKLAFSFYAEIKPLVKPTKDIMRVHISVYRWHEFFPHAKSNMGVVIALWSPVILVYFMDTQIWYAIVSTLVGGLNGAFRRLGEIRTLGMLRSRFQSLPEAFNACLVPNEKSETPKKKGIMATFTRKFDQVPSSKDKEAARFAQMWNKIISSFREEDLISDREMELLLVPYWADRDLDLIRWPPFLLASKIPIALDMAKDSNGKDRELTKRLSVDSYMTCAVRECYASFKNLINFLVVGEREGQV-INEIFSRIDEHIEKETLIKDLNLSALPDLYGQFVRLIEYLME-NREEDKDQIVIVLLNMLEVVTRDIMDEEVPSMLESTHNGTYVKYDVMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFSNSLFMEMPDAPKIRNMLSFSVLTPYYSEDVLFSIFGLEKQNEDGVSILFYLQKIFPDEWTNFLERV---KCGSEEELRAREELEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEELMKGYKALELTSEDASKSGTSLWAQCQALADMKFTFVVSCQQYSVQKRSGDQRAKDILRLMTTYPSLRVAYIDEVEQTHKESYKGADEKIYYSALVKAAPQTKSMDSSESVQTLDQVIYRIKLPGPAILGEGKPENQNHSIIFTRGEGLQTIDMNQDNYMEEAFKMRNLLQEFLVKHGGVRTPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQRVLASPLKVRFHYGHPDVFDRLFHLTRGGVCKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSNQKAFRSNMPLQAALASQSFVQIGFLMALPMMMEIGLERGFHNALIDFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGHAYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKEIGHLRHSGKRGIILEIVLALRFFIFQYGLVYQLSTFKQENQSLWIYGASWFVILFILLIVKGLGVGRQRFSTNFQLLFRIIKGFVFLTFLGLLITFLALRFLTPKDIFLCMLAFMPTGWGMLLIAQACKPLIQRLGFWSSVRTLARGYEILMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGQRKD 1943          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy Swiss-Prot
Match: CALS3_ARATH (Callose synthase 3 OS=Arabidopsis thaliana GN=CALS3 PE=2 SV=3)

HSP 1 Score: 1534.24 bits (3971), Expect = 0.000e+0
Identity = 882/1956 (45.09%), Postives = 1231/1956 (62.93%), Query Frame = -2

Query:  233 VPSSLNNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDVARLLEFYRLYREK------NNVDKLREEEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQLTEEIPVELKRVI----DSDAAMTEDLIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSIPETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQP-VW-SNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIA-------QPANSCSKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWRKTS-FFQKP--------EPRSKNPLKLGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFNDGH----LNAKTLREVLSLGPTFVVMKFTESVLDVIMMYGAYST----TRRLAVSRIFLRFIWFSIASVFVSF------LYVRALQE--ESKPNSNSVVFRXXXXXXXXXXXIHFFISFLMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLHPVRXXXXXXXXXXXXXKF-------------DAARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGK-LPLVQWPLFLLASKIFLAKDIAAESRDSQDELWERISRDEYMKYAVQECYYALRYILTAILEAE-GRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGIL--NQAEKPEHEKGAVNAVQDLYDVVRHDVL-------AIYLREHSDQWQS-ILKARTEGRLFAK---LNWPRDPELKA---QVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLE---RENARDTEAALSRLETTDTQG-YELSPEARARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFIDVVE-TLKDGKVHTE---YYSKLVKAD--------INGKDKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDH-GIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYGYN-DXXXXXXXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIR--TMRGRILETILSLRFFLFQYGVVYKLNVQGTNTSLTVYGFSWVVLAVLIILFKVFTFS-QKMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGNNPN 5812
            VPSSL   +I  ILRVA+E++   P VA +   +A+  A  LDP S GRGV QFKT L+  ++++     +G + +S D   +  FY+ Y +K      N  DK    ++                       + + T  VL  VL+ +     +E+ R I    D  A  T+  + YNI+P  LD  +   AI+ +PE++AAV AL++   LP  P      + +  D++D+L  +FGFQKDNV+NQREH++LLLAN   R     + +PKLD+ A+ +V  K   NY KWC YL  +  +W   +    +++K+L+++LY LIWGEAAN+RF+PECLCYI+HHMA E+   L  +++       +PA    +D   +FL +V+TP+Y+V+  EA  ++ G++ HS WRNYDD NEYFWS+ CF L WP R  + FF  P        +  +  P+ +   +  GK +FVE R+F+H++ SF R+W F ++  Q + I+A++ G       A   ++VLS+  T  +MK  ++VLDVI+ + A+ +     +   + ++F    W  I  V  ++       + R ++    S  +S S+      I +  Y   +     +   P   R  E   N  ++  + W  Q R YVGRGM+E      KY +FW+++++ K AF+Y+ +I+PLV PT+ ++      + WH+F  R  +N   V++LWAP+I +Y +D  I+Y + S ++G + GA  RLGEIR+L  ++  FE  P AF  RL P   +      +                   +AARF+  WN II + REED +++ EM+LL +P    + L L+QWP FLLASKI +A D+A +S     EL +RI  D YMK AV+ECY + + I+  +++    +  +E I+  ++  I    +  +++++ L  +      L+  L  N+ E  +H    V   QD+ +VV  D++       ++    H   W   ++    + +LFA    + +P +P  +A   ++KR+Y LLT K+SA +VP NLEARRR+ FF+NSLFMDMP A  V+ MLSFSV TPYY+E VL+S+ +L   NEDG+SILFYLQKI+PDEW NFL R+   +  +E EL +S     ELR WASYRGQTL RTVRGMMYYRKAL LQ +L+    E+  +   A+      +++G   L  + +A AD+KFTYVV+CQ YG  K    P A DI  LM R  +LRVA+ID VE  +KD         YYS LVK              D+ IY I+LPG   LGEGKPENQNHAI+F+RG  +QTIDMNQDNY EEALK+RNLL+EF   H G+R P+ILG+REH+FTGSVSSLA FMSNQE+SFVT+GQR+LA PL+VR HYGHPDVFDR+FH+TRGG+SKAS++IN+SEDI+AGFNSTLR+GN+THHEYIQVGKGRDVGLNQI++FE K+A GNGEQ LSRD+YRLG  FDFFRMMS YFTTVGFYF T++TVLT+YIFLYGR YL LSG+ + +  +  I DN  L+ AL +Q   QIG   A+PM++   LE+GF  A+  F+ MQLQL  VFFTFSLGT+THY+GRT+LHGGA+Y++TGRGFVV H KF++NYRLYSRSHFVKGLE++LLLVVY  +G      L+Y+L++IS WFM  +WLFAP+LFNPSGFEWQKIV+D+ DW  W+   GGIGV  E+SWE+WW+EE  H+R    RG ++E +L+LRFF++QYG+VY L +     +  VYG SW+V+ +++ + K  +   ++ S +FQL+ R I+G+ FM  IA + + + L  ++I DI   ILAF+PTGWG+L IA A KP V + G W SVR++AR Y+  MG+++F PVAF +WFPFVS FQTR++FNQAFSRGL+IS IL G+  +
Sbjct:   38 VPSSL--VEIAPILRVANEVESSNPRVAYLCRFYAFEKAHRLDPTSSGRGVRQFKTALLQRLEREHDPTLMGRVKKS-DAREMQSFYQHYYKKYIQALHNAADKADRAQL----------------------TKAYQTANVLFEVLKAVNLTQSIEVDREILEAQDKVAEKTQLYVPYNILP--LDPDSANQAIMRYPEIQAAVLALRNTRGLP-WPEGHK--KKKDEDMLDWLQEMFGFQKDNVANQREHLILLLANVHIRQFPKPDQQPKLDDQALTEVMKKLFKNYKKWCKYLGRKSSLWLPTIQQEMQQRKLLYMALYLLIWGEAANLRFMPECLCYIYHHMAFELYGMLAGNVSPMTGENVKPAYGGEED---AFLRKVVTPIYEVIQMEAQRSKKGKSKHSQWRNYDDLNEYFWSVDCFRLGWPMRADADFFCLPVAVPNTEKDGDNSKPI-VARDRWVGKVNFVEIRSFWHVFRSFDRMWSFYILCLQAMIIMAWDGGQPSSVFGADVFKKVLSVFITAAIMKLGQAVLDVILNFKAHQSMTLHVKLRYILKVFSAAAWVIILPVTYAYSWKDPPAFARTIKSWFGSAMHSPSLFI----IAVVSYLSPNMLAGVMFLFPLLRRFLERS-NYRIVMLMMWWSQPRLYVGRGMHESAFSLFKYTMFWVLLIATKLAFSYYIEIRPLVAPTQAIMKARVTNFQWHEFFPRAKNNIGVVIALWAPIILVYFMDSQIWYAIFSTLFGGIYGAFRRLGEIRTLGMLRSRFESLPGAFNDRLIPDGKNQQKKKGIRATLSHNFTEDKVPVNKEKEAARFAQLWNTIISSFREEDLISDREMDLLLVPYWADRDLDLIQWPPFLLASKIPIALDMAKDSNGKDRELKKRIESDTYMKCAVRECYASFKNIIKFVVQGNREKEVIEIIFAEVDKHIDTGDLIQEYKMSALPSLYDHFVKLIKYLLDNKEEDRDH---VVILFQDMLEVVTRDIMMEDYNISSLVDSSHGGTWHGGMIPLEQQYQLFASSGAIRFPIEPVTEAWKEKIKRIYLLLTTKESAMDVPSNLEARRRISFFSNSLFMDMPMAPKVRNMLSFSVLTPYYTEEVLFSLRDLETPNEDGVSILFYLQKIFPDEWNNFLERV---KCLSEEELKESDELEEELRLWASYRGQTLTRTVRGMMYYRKALELQAFLDMAMHEDLMEGYKAVELNSENNSRGERSLWAQCQAVADMKFTYVVSCQQYGIHKRSGDPRAQDILRLMTRYPSLRVAYIDEVEEPVKDKSKKGNQKVYYSVLVKVPKSTDHSTLAQNLDQVIYRIRLPGPAILGEGKPENQNHAIIFSRGEGLQTIDMNQDNYMEEALKMRNLLQEFLTKHDGVRHPSILGLREHIFTGSVSSLAWFMSNQETSFVTIGQRLLANPLRVRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDIYRLGHRFDFFRMMSCYFTTVGFYFSTLITVLTVYIFLYGRLYLVLSGLEQGLSTQKGIRDNTPLQIALASQSFVQIGFLMALPMLMEIGLERGFRTALSEFVLMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAKYRSTGRGFVVFHAKFADNYRLYSRSHFVKGLEMMLLLVVYQIFGSAYRGVLAYLLITISMWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWNKWINNIGGIGVPAEKSWESWWEEEQEHLRYSGKRGIVVEILLALRFFIYQYGLVYHLTITEKTKNFLVYGVSWLVIFLILFVMKTVSVGRRRFSASFQLMFRLIKGLIFMTFIAIIVILITLAHMTIQDIIVCILAFMPTGWGMLLIAQACKPVVHRAGFWGSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGHRKD 1948          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy Swiss-Prot
Match: CALS1_ARATH (Callose synthase 1 OS=Arabidopsis thaliana GN=CALS1 PE=1 SV=2)

HSP 1 Score: 1533.47 bits (3969), Expect = 0.000e+0
Identity = 887/1968 (45.07%), Postives = 1231/1968 (62.55%), Query Frame = -2

Query:  233 MGSVGHAAGGIAGYVPSSLNNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQ--DVARLLEFYRLYREK------NNVDKLREEEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQL--TEEIPV--ELKRVIDSDAAMTEDLIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSIPETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQP-VW-SNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIA-------QPANSCSKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWRKTS--FFQKPEPR--SKNPLKLGGGQH-RGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFN-DGHLNA----KTLREVLSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIASVFVSFLYVRALQEES-------------KPNSNSVVFRXXXXXXXXXXXIHFFISFLMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLHPVRXXXXXXXX---XXXXXKFD---------AARFSPFWNEIIKNLREEDYLTNFEMELLFMPK-NTGKLPLVQWPLFLLASKIFLAKDIAAESRDSQDELWERISRDEYMKYAVQECYYALRYILTAIL--EAEGRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGILNQAEKPEHEKGAVNAVQDL----YDVVRHDVLAIYLREHSD---QWQSILKARTEGRLFAKLNWP---RDPELKAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEAALSRLETTDTQ----GYELSPEARARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFIDVVE-TLKDGKVHTE---YYSKLVKADINGK-----------DKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDHG-IRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYGYN-DXXXXXXXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIR--TMRGRILETILSLRFFLFQYGVVYKLNV-QGTNTSLTVYGFSW-VVLAVLIILFKVFTFSQKMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGNNPN 5854
            +GS+G A    +  VPSSL   +I  ILRVA+E++   P VA +   +A+  A  LDP S GRGV QFKT L+    Q+L +    T+   Q  D   +  FY+ Y +K      N  DK    ++                       + + T  VL  VL+ +  TE++ V  E+    +     T+  + YNI+P  LD  +   AI+  PE++AAV+AL++   LP         +    D++D+L  +FGFQKDNV NQREH++LLLAN   R     + +PKLD+ A+  V  K   NY KWC YL  +  +W   +    +++K+L++ LY LIWGEAAN+RF+PECLCYI+HHMA E+   L   ++       +PA     +   +FL +V+TP+Y  ++ EA  +  G++ HS WRNYDD NEYFWS+ CF L WP R  +  F Q  E     ++ +K   G    GK +FVE R+F+H++ SF RLW F ++  Q + +IA+N  G L+A        +VLS+  T  ++K  ++VLD+ + + A  +       R  ++    ++  V ++  Y  + +  S               NS S+      + I IY   +   + L   P   R  E  D   ++  + W  Q R Y+GRGM+E      KY +FW+++L  K AF+Y+ +IKPLV PT+ ++ +    YSWH+F     +N   V++LW+PVI +Y +D  I+Y ++S + G L GA  RLGEIR+L  ++  F+  P AF   L P   S  +  +        KFD         AARF+  WN+II + REED +++ EMELL +P  +   L L++WP FLLASKI +A D+A +S     EL +R++ D YM  AV+ECY + + ++  ++  E EG+  +  I+  I+  I K+T+  +  L+ L  +  +   L+  L +  + + ++  +  +  L     D++  +V ++    H+    ++  +     + + F++L +P   +    K ++KRL+ LLT+K+SA +VP NLEARRRL FF+NSLFMDMPPA  ++ MLSFSV TPY+SE VL+S+  L ++NEDG+SILFYLQKI+PDEW NFL R+   +   E EL        ELR WASYRGQTL +TVRGMMYYRKAL LQ +L+     +       LE T  +    G  L  + +A AD+KFT+VV+CQ Y   K      A DI  LM    ++RVA+ID VE T K+    TE   YYS LVKA    K           D+ IY IKLPG   LGEGKPENQNHAI+FTRG  +QTIDMNQDNY EEA K+RNLL+EF   HG +R PTILG+REH+FTGSVSSLA FMSNQE+SFVT+GQRVLA+PLKVR HYGHPD+FDR+FH+TRGGI KAS++IN+SEDI+AGFNSTLR+GN+THHEYIQVGKGRDVGLNQI++FE K+A GNGEQ LSRD+YRLG  FDFFRM+S YFTT+GFYF TMLTVLT+Y+FLYGR YL LSG+ E +  +    +N  LEAAL +Q   QIG   A+PM++   LE+GF  A++ F+ MQLQL +VFFTF LGT+THY+GRT+ HGGA Y+ TGRGFVV H KF+ENYR YSRSHFVKG+E+++LL+VY  +G +    ++YIL+++S WFM ++WLFAP+LFNPSGFEWQKIV+D+ DW  W++ RGGIGV  E+SWE+WW++E+ H+R   +RG  LE  L+LRFF+FQYG+VY L+  +G N S  VYG SW V+L +L+I+  +    ++ S NFQLL R I+G+ F+  +A +   +AL  ++I D+F  +LAF+PTGWG+L IA A KP +++ G+W SVR++AR Y+  MG+++F PVAF +WFPFVS FQTR++FNQAFSRGL+IS IL G   +
Sbjct:   22 VGSLGEAMLD-SEVVPSSLV--EIAPILRVANEVEASNPRVAYLCRFYAFEKAHRLDPTSSGRGVRQFKTALL----QRLERENETTLAGRQKSDAREMQSFYQHYYKKYIQALLNAADKADRAQL----------------------TKAYQTAAVLFEVLKAVNQTEDVEVADEILETHNKVEEKTQIYVPYNILP--LDPDSQNQAIMRLPEIQAAVAALRNTRGLPWTAGH---KKKLDEDILDWLQSMFGFQKDNVLNQREHLILLLANVHIRQFPKPDQQPKLDDRALTIVMKKLFRNYKKWCKYLGRKSSLWLPTIQQEVQQRKLLYMGLYLLIWGEAANLRFMPECLCYIYHHMAFELYGMLAGSVSPMTGEHVKPAYGGEDE---AFLQKVVTPIYQTISKEAKRSRGGKSKHSVWRNYDDLNEYFWSIRCFRLGWPMRADADFFCQTAEELRLERSEIKSNSGDRWMGKVNFVEIRSFWHIFRSFDRLWSFYILCLQAMIVIAWNGSGELSAIFQGDVFLKVLSVFITAAILKLAQAVLDIALSWKARHSMSLYVKLRYVMKVGAAAVWVVVMAVTYAYSWKNASGFSQTIKNWFGGHSHNSPSLFI----VAILIYLSPNMLSALLFLFPFIRRYLERSD-YKIMMLMMWWSQPRLYIGRGMHESALSLFKYTMFWIVLLISKLAFSYYAEIKPLVGPTKDIMRIHISVYSWHEFFPHAKNNLGVVIALWSPVILVYFMDTQIWYAIVSTLVGGLNGAFRRLGEIRTLGMLRSRFQSIPGAFNDCLVPQDNSDDTKKKRFRATFSRKFDQLPSSKDKEAARFAQMWNKIISSFREEDLISDREMELLLVPYWSDPDLDLIRWPPFLLASKIPIALDMAKDSNGKDRELKKRLAVDSYMTCAVRECYASFKNLINYLVVGEREGQV-INDIFSKIDEHIEKETLITELNLSALPDLYGQFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSYVKYDVMTPLHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFSNSLFMDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFPDEWTNFLERV---KCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEELLKGYKALELTSEEASKSGGSLWAQCQALADMKFTFVVSCQQYSIHKRSGDQRAKDILRLMTTYPSIRVAYIDEVEQTHKESYKGTEEKIYYSALVKAAPQTKPMDSSESVQTLDQLIYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQDNYMEEAFKMRNLLQEFLEKHGGVRCPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQRVLASPLKVRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMALPMMMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRGITLEIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGASWFVILFILLIVKGLGVGRRRFSTNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDLFICMLAFMPTGWGMLLIAQACKPLIQQLGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGQRKD 1943          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy Swiss-Prot
Match: CALS6_ARATH (Putative callose synthase 6 OS=Arabidopsis thaliana GN=CALS6 PE=3 SV=2)

HSP 1 Score: 1508.81 bits (3905), Expect = 0.000e+0
Identity = 852/1910 (44.61%), Postives = 1208/1910 (63.25%), Query Frame = -2

Query:  245 VPSSLNNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDR--SQDVARLLEFYRLYREKNNVDKLREEEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQL--TEEIPVELKRVIDSDAAMTEDLIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPK--------LPSDFSIPETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQPVWSNLDAVNKEK-KILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHI-AQPANSCSKDGVV---SFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWR-KTSFFQKPE---PRSKNPLKLGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFN-DGHL----NAKTLREVLSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIASVFVSFLYVRALQEESKPNSNSVVFRXXXXXXXXXXXIHFFISF----------LMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLHPVRXXXXXXXXXXXXXKFDAARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGKLPLVQWPLFLLASKIFLAKDIAAESRDSQD-ELWERISRDEYMKYAVQECYYALRYILTAILEAEG-RTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGIL--NQAEKPEHEKGAVNAVQDLYDVVRHDVL--AIYLREHSDQWQSILKARTEGRLFAKLN--WPRDPELKAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEAALSRLETTDTQGYELSPEARARADLKFTYVVTCQIYGRQKE----EQKPEAADIALLMQRNEALRVAFIDVVETLKDGKVHTEYYSKLVKADINGKDKEIYAIKLPGDP-KLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDH-GIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYGYN-DXXXXXXXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIR--TMRGRILETILSLRFFLFQYGVVYKLNVQGTNTSLTVYGFSWVVLAVLIILFKVFTFS-QKMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAG 5812
            VPSSL +  I  ILRVA+EI+ + P VA +   HA+  A  +D  S GRGV QFKT L+     +L K E  T  +    D   +  +Y+ + EK            ++E        GE  RK  +  R++    VL  VL+ +  + ++  E +R  +      +    YNI+P  L A     AIV  PEV+AA SA+++  +LP+         P++     T+  D++++L   FGFQ+ NV+NQREH++LLLAN   R    EE + +L  + V ++  K+  +Y  WC YL         D  +K++ +++++SLY LIWGEA+N+RF+PEC+CYIFH+MA ++   L  ++ A    +   + V+   SFL  VITP+Y V+  EA  N+ G A HS WRNYDD NEYFWS  CF + WP   K  FF   +   P+ +   ++  G+ + KT+FVE RTF++L+  F R+WIFLVM FQ + I+ ++  G L    +    + VL++  T   +   ++ LD+I+ + A+   +   + R  L+F    + +V +   Y +++Q   +P      F  +           + +SF          L  +P   R  E C ++  I+ I W  Q + YVGRGM+E      KY  FW+++L  K AF Y+ +I PL+ PT+ ++ +    Y WH+F     +N   V+++WAP++ +YL+D  I+Y + S ++G + GA   LGEIR+L  ++  FE  P AF + L P   +    ++     K +   FS  WNE I ++R ED +++ + +LL +P ++G + ++QWP FLLASKI +A D+A + +  +D EL+ +I  D YM YAV E Y  L+ I+ A+LE E  R  + +++  ++ S+ ++    +F+++ L L+  ++   L IL  +  ++  ++   +N  QD+ +++  D+L     + E +      +K   + + F K+N    RD   + +V RL+ LL++K+SA NVP+NLEARRR+ FF NSLFM+MP A  +++MLSFSV TPYY E VLYS  +L K+NEDGISILFYLQKIYPDEW N+L R+ +D    E +  +       LR W SYRGQTLARTVRGMMYYR+AL LQ Y E    +   +    + + D         ARA ADLKFTYVV+CQ+YG QK+      +    +I  LM +  +LRVA++D  E   D K    +YS L+K   +  D+EIY IKLPG P ++GEGKPENQNHAI+FTRG A+QTIDMNQDNYFEEA K+RN+LEEF+++  G R PTILG+REH+FTGSVSSLA FMSNQESSFVT+GQR+LA PL+VR HYGHPD+FDR+FHITRGG+SKAS++IN+SEDI+ GFNSTLR G +THHEYIQVGKGRDVGLN I++FE KVA GNGEQ LSRDVYRLG  FDF+RM+SFYFTT+GFYF +MLTVLT+Y FLYGR Y+ +SG+ + +   A      ALE AL TQ +FQ+G    +PMV+   LE GF  AIV F  MQLQL +VFFTF LGT++HY+GRTILHGG++Y+ TGRGFVV H KF+ENYRLYSRSHFVKGLE++LLLVVY  YG++  S+  Y+ +++S WFM  SWLFAP++FNPSGFEWQK V+D+ DW  WL  RGGIG+  E+SWE+WW+ E  H++  ++RGRILE  L+LRFF++QYG+VY+LN+   + S  VYG SWVVL   +++ K+ +   ++   +FQL+ R ++ + F+  ++ + +   +  L++ D+ AS+LAF+PTGW IL I    +  +K  G+W SV+ + R Y+  MG++IF P+A  SWFP VS FQ RL+FNQAFSRGL+IS+ILAG
Sbjct:   43 VPSSLAS--IAPILRVANEIEKDNPRVAYLCRFHAFEKAHRMDATSSGRGVRQFKTYLL----HRLEKEEEETKPQLAKNDPREIQAYYQNFYEK-----------YIKE--------GETSRKPEEMARLYQIASVLYDVLKTVVPSPKVDYETRRYAEEVERKRDRYEHYNILP--LYAVGTKPAIVELPEVKAAFSAVRNVRNLPRRRIHLPSNTPNEMRKARTKLNDILEWLASEFGFQRGNVANQREHIILLLANADIRKRNDEEYD-ELKPSTVTELMDKTFKSYYSWCKYLHSTSNLKFPDDCDKQQLQLIYISLYLLIWGEASNVRFMPECICYIFHNMANDVYGILFSNVEAVSGETYETEEVIDEESFLRTVITPIYQVIRNEAKRNKGGTASHSQWRNYDDLNEYFWSKKCFKIGWPLDLKADFFLNSDEITPQDERLNQVTYGKSKPKTNFVEVRTFWNLFRDFDRMWIFLVMAFQAMVIVGWHGSGSLGDIFDKDVFKTVLTIFITSAYLTLLQAALDIILNFNAWKNFKFSQILRYLLKFAVAFMWAVLLPIAYSKSVQ---RPTGVVKFFSTWTGDWKDQSFYTYAVSFYVLPNILAALLFLVPPFRRAME-CSDMRPIKVIMWWAQPKLYVGRGMHEDMFSLFKYTTFWIMLLISKLAFNYYVEILPLITPTKMIMNLHIGHYQWHEFFPHATNNIGVVIAIWAPIVLVYLMDTQIWYAIFSTLFGGIHGAFSHLGEIRTLGMLRSRFESIPIAFSRTLMPSEDAKRKHADDYVDQK-NITNFSQVWNEFIYSMRSEDKISDRDRDLLLVPSSSGDVSVIQWPPFLLASKIPIAVDMAKDFKGKEDAELFRKIKSDSYMYYAVIESYETLKKIIYALLEDEADRRVMNQVFLEVDMSMQQQRFIYEFRMSGLPLLSDKLEKFLSILLSDYEDQGTYKSQLINVFQDVIEIITQDLLVNGHEILERARVHSPDIKNEKKEQRFEKINIHLVRDRCWREKVIRLHLLLSVKESAINVPQNLEARRRITFFANSLFMNMPSAPRIRDMLSFSVLTPYYKEDVLYSEEDLNKENEDGISILFYLQKIYPDEWTNYLDRL-KDPKLPEKDKSEF------LREWVSYRGQTLARTVRGMMYYRQALELQCYQEVAGEQAEFSVFRAMASNDENQKAFLERARALADLKFTYVVSCQVYGNQKKSGDIHNRSCYTNILQLMLKYPSLRVAYVDEREETADAKSPKVFYSVLLKGG-DKFDEEIYRIKLPGPPAEIGEGKPENQNHAIIFTRGEALQTIDMNQDNYFEEAFKLRNVLEEFNKERVGRRKPTILGLREHIFTGSVSSLAWFMSNQESSFVTIGQRILANPLRVRFHYGHPDIFDRIFHITRGGVSKASKVINLSEDIFGGFNSTLRGGYVTHHEYIQVGKGRDVGLNPISIFEAKVANGNGEQTLSRDVYRLGHRFDFYRMLSFYFTTIGFYFSSMLTVLTVYAFLYGRMYMVMSGLEKEILRLASPNQLEALEQALATQSIFQLGFLMVLPMVMEIGLEHGFRSAIVDFFIMQLQLASVFFTFQLGTKSHYYGRTILHGGSKYRPTGRGFVVFHAKFAENYRLYSRSHFVKGLELLLLLVVYQIYGHSYRSSNLYLYITVSMWFMVGSWLFAPFIFNPSGFEWQKTVDDWTDWKRWLGDRGGIGIPVEKSWESWWNVEQEHLKHTSIRGRILEITLALRFFIYQYGIVYQLNISQRSKSFLVYGLSWVVLLTSLLVLKMVSMGRRRFGTDFQLMFRILKALLFLGFLSVMTILFVVFKLTLTDLSASVLAFLPTGWAILLIGQVLRSPIKALGVWDSVKELGRAYENIMGLVIFAPIAVLSWFPIVSEFQARLLFNQAFSRGLQISMILAG 1911          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy Swiss-Prot
Match: CALS7_ARATH (Callose synthase 7 OS=Arabidopsis thaliana GN=CALS7 PE=3 SV=3)

HSP 1 Score: 1481.85 bits (3835), Expect = 0.000e+0
Identity = 851/1914 (44.46%), Postives = 1203/1914 (62.85%), Query Frame = -2

Query:  245 VPSSLNNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDVARLLEFYRLYREKNNVDKLREEEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQLTEEIPVELK--RVIDSDAAMTEDLIAYNIIP-FPLDAPTITNAIVSFPEVRAAVSALKHYSSLPK---LPSDFSIPET-----RS-PDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYL-CIQPVWSNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIAQPANSCSKDGVV---SFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWR-KTSFFQKPEPRSKNPLK----LGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFN-DGHLNA----KTLREVLSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIASVFVSFLYVRALQEESKPNSNSVVFRXXXXXXXXXXXIHFFISF----------LMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLHPVRX-XXXXXXXXXXXXKFDAARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGKLPLVQWPLFLLASKIFLAKDIAAESRDSQD-ELWERISRDEYMKYAVQECYYALRYILTAIL--EAEGRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGIL-NQAEKPEHEKGAVNAVQDLYDVVRHDVLA----IYLREHSDQWQSILKARTEGRLFAK--LNWPRDPELKAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGR-DENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEAALSRLETTDTQGYELSPEARARADLKFTYVVTCQIYGRQKEEQKPEA----ADIALLMQRNEALRVAFIDVVETLKDGKVHTEYYSKLVKADINGKDKEIYAIKLPGDP-KLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDH-GIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYGYN-DXXXXXXXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIR--TMRGRILETILSLRFFLFQYGVVYKLNVQGTNTSLTVYGFSW-VVLAVLIILFKVFTFSQKMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAG 5812
            VPSSL +  I  ILRVA++I  +   VA +   HA+  A  +DP S GRGV QFKT L+  ++++    E   +    D   +  +Y+ + E N  D                   GE ++   +  +++    VL  VL+ +  +  ++ K  R         E    YNI+P + L A T   A++  PE++AA+ A+ +  +LP+     +  ++ E      RS  D++++L  VFGFQ+ NV+NQREH++LLLAN   R     E   ++  + V+K+  K   NY  WC YL C   +        ++  +L++ LY LIWGEA+N+RF+PECLCYIFH+MA E+   L  ++        + G     +FL  VITP+Y V+  E   N+NG+A HS WRNYDD NEYFW   CF L WP   K  FF   +  S+ P +    +  G+ + KT+FVE RTF++LY SF R+W+FLV+  Q + I+A++  G + A       R VL++  T   +   ++ LD+++ +GA+ + +   + R   +F+  ++ ++ +   Y +++Q    P      F  ++          + I+              +P   R+ E   N+ ++  I W  Q + Y+GRGM+E      KY  FW+++L  K AF+Y+ +I PLV PT+ +  M  + Y WH+F     HN   ++++W P++ +Y +D  I+Y + S ++G + GA   LGEIR+L  ++  F+  P AF  +L P+    A          + D ARFS  WN+ I  +R+ED +++ E +LL +P ++G + +VQWP FLLASKI +A D+A + +  +D +L+++I  + YM YAV E Y  +R I+  +L  E++ R   E  YE ++ SI +     +F++  + L+  ++   L IL +  E+ +++   +N +QD+ +++  DV+     I  R H  Q   I   + E R F K  L+  ++   + +V RL  LLT+K+SA N+P++LEARRR+ FF NSLFM+MP A  V++MLSFSV TPYY E VLYS  EL K+NEDGI+ILFYLQ+IYP+EW N+  R+     N +E +  +      +LR W SYRGQTL+RTVRGMMYYR AL LQ + E      T       E+ +      S  ARA ADLKFTYVV+CQ+YG QK+  +        +I  LM +  +LRVA+ID  E   +GK    +YS L+K   +  D+EIY IKLPG P ++GEGKPENQNHAI+FTRG A+QTIDMNQDNYFEE  K+RN+L+EFD    G R PTILG+REH+FTGSVSSLA FMSNQE+SFVT+GQRVLA PL+VR HYGHPD+FDR+FHITRGGISKAS+IIN+SEDI+AG+NSTLR G +THHEYIQ GKGRDVG+NQI+ FE KVA GNGEQ LSRDVYRLG+ FDF+RM+SFYFTTVGFYF +M+TVLT+Y+FLYGR YL LSG+ + +   A + ++ ALE AL  Q +FQ+G    +PMV+   LE+GF  A+  FI MQLQL +VFFTF LGT+ HYFGRTILHGG++Y+ATGRGFVV H KF+ENYRLYSRSHFVKGLE+V+LLVVY  YG +  S+ +Y+ ++ S WF+  SWLFAP++FNPSGFEWQK V+D+ DW  W+  RGGIG+  ++SWE+WWD E  H++   +RGR+LE +L+LRF L+QYG+VY LN+   +T+  VYG SW ++L+VL++L  V    +K   +FQ++ R ++ + F+  ++ + V   +  L+I D+FASILAF+PTGW IL I  A +   K  G W SV+ + R Y+  MG++IF P+A  SWFPFVS FQTRL+FNQAFSRGL+IS+ILAG
Sbjct:   51 VPSSLAS--IAPILRVANDIDQDNARVAYLCRFHAFEKAHRMDPTSSGRGVRQFKTYLLHKLEEEEEITE--HMLAKSDPREIQLYYQTFYENNIQD-------------------GEGKKTPEEMAKLYQIATVLYDVLKTVVPQARIDDKTLRYAKEVERKKEQYEHYNILPLYALGAKT---AVMELPEIKAAILAVCNVDNLPRPRFHSASANLDEVDRERGRSFNDILEWLALVFGFQRGNVANQREHLILLLANIDVRKR-DLENYVEIKPSTVRKLMEKYFKNYNSWCKYLRCDSYLRFPAGCDKQQLSLLYIGLYLLIWGEASNVRFMPECLCYIFHNMANEVHGILFGNVYPVTGDTYEAGAPDEEAFLRNVITPIYQVLRKEVRRNKNGKASHSKWRNYDDLNEYFWDKRCFRLKWPMNFKADFFIHTDEISQVPNQRHDQVSHGKRKPKTNFVEARTFWNLYRSFDRMWMFLVLSLQTMIIVAWHPSGSILAIFTEDVFRNVLTIFITSAFLNLLQATLDLVLSFGAWKSLKFSQIMRYITKFLMAAMWAIMLPITYSKSVQ---NPTGLIKFFSSWVGSWLHRSLYDYAIALYVLPNILAAVFFLLPPLRRIMERS-NMRIVTLIMWWAQPKLYIGRGMHEEMFALFKYTFFWVMLLLSKLAFSYYVEILPLVNPTKLIWDMHVVNYEWHEFFPNATHNIGVIIAIWGPIVLVYFMDTQIWYAIFSTLFGGIYGAFSHLGEIRTLGMLRSRFKVVPSAFCSKLTPLPLGHAKRKHLDETVDEKDIARFSQMWNKFIHTMRDEDLISDRERDLLLVPSSSGDVTVVQWPPFLLASKIPIALDMAKDFKGKEDVDLFKKIKSEYYMHYAVVEAYETVRDIIYGLLQDESDKRIVREICYE-VDISIQQHRFLSEFRMTGMPLLSDKLEKFLKILLSDYEEDDYKSQIINVLQDIIEIITQDVMVNGHEILERAHL-QSGDIESDKKEQR-FEKIDLSLTQNISWREKVVRLLLLLTVKESAINIPQSLEARRRMTFFANSLFMNMPDAPRVRDMLSFSVLTPYYKEDVLYSEEELNKENEDGITILFYLQRIYPEEWSNYCERVNDLKRNLSEKDKAE------QLRQWVSYRGQTLSRTVRGMMYYRVALELQCFQEYTEENATNGGYLPSESNEDDRKAFSDRARALADLKFTYVVSCQVYGNQKKSSESRDRSCYNNILQLMLKYPSLRVAYIDEREETVNGKSQKVFYSVLLKG-CDKLDEEIYRIKLPGPPTEIGEGKPENQNHAIIFTRGEALQTIDMNQDNYFEECFKMRNVLQEFDEGRRGKRNPTILGLREHIFTGSVSSLAWFMSNQETSFVTIGQRVLANPLRVRFHYGHPDIFDRIFHITRGGISKASKIINLSEDIFAGYNSTLRGGYVTHHEYIQAGKGRDVGMNQISFFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYFSSMITVLTVYVFLYGRLYLVLSGLEKNILQSASVHESNALEQALAAQSVFQLGFLMVLPMVMEIGLEKGFRTALGDFIIMQLQLASVFFTFQLGTKAHYFGRTILHGGSKYRATGRGFVVFHAKFAENYRLYSRSHFVKGLELVILLVVYQVYGTSYRSSSTYMYITFSMWFLVTSWLFAPFIFNPSGFEWQKTVDDWTDWKRWMGNRGGIGIVLDKSWESWWDIEQEHLKHTNLRGRVLEILLALRFLLYQYGIVYHLNIARRHTTFLVYGLSWAILLSVLLVLKMVSMGRRKFGTDFQVMFRILKALLFLGFLSVMTVLFVVCGLTISDLFASILAFLPTGWAILLIGQALRSVFKGLGFWDSVKELGRAYEYIMGLVIFTPIAVLSWFPFVSEFQTRLLFNQAFSRGLQISMILAG 1923          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy Swiss-Prot
Match: CALS8_ARATH (Putative callose synthase 8 OS=Arabidopsis thaliana GN=CALS8 PE=2 SV=2)

HSP 1 Score: 1456.43 bits (3769), Expect = 0.000e+0
Identity = 825/1956 (42.18%), Postives = 1184/1956 (60.53%), Query Frame = -2

Query:  233 VPSSLNNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDVARLLEFYRLYREKNNVDKLREEEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQLTEEIPVELKRVIDSDAAMTEDLIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSIPETRSP--DLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLC----IQPVWSNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIA----QPANSCSKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWR-KTSFFQKPEPRSKNPLKLGG--------------------------------GQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFNDGH-----LNAKTLREVLSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIASVFVSFLYVRALQEESKPNSNSVVFRXXXXXXXXXXXIHFFIS------FLMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLHPVRXXXXXXXXXXXXXKFD-----------AARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGKLP-LVQWPLFLLASKIFLAKDIAAESRDSQDELWERISRDEYMKYAVQECYYALRYILTAILEAE-GRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGILN-------QAEKPEHEKGA-VNAVQDLYDVVRHDVLA--------IYLREHSDQWQSILKARTEGRLFAKLN-W-------PRDPELKAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDE-NAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEAALSRLETTDTQGYELSPEARARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFIDVVETLKDGKVHTEYYSKLVKADINGKDKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYGYNDXXXXXXX-XXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIR--TMRGRILETILSLRFFLFQYGVVYKLNVQGTNTSLTVYGFSWVVLAVLIILFKVFTFSQKM-SVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGNNPN 5812
            +P++L + +I   LR+A+ ++ EEP +A +   HA+ +A ++D NS GRGV QFKT L+    Q+L   E  T+ R ++ + + E  R+Y           +E ++R    F  NL   +R+ L   R   +  VL  VL+ +T     +     +S  A +E  + YNI+P  LD   +  AI+  PE++AAV+ +++   LP  P +F   +   P  DL +FL Y FGFQ  NV+NQREH++LLL+N   R    +   PK  + AV  +  K   NY  WC +L     I+  +   +A+  + K L++ LY LIWGEA+N+RF+PECLCYIFHHMA E+   L   ++    +        G  SFL  V+TP+Y VV  EA  N+NG A HS WRNYDD NE+FWSL CF++ WP R +  FF      +  P +  G                                 +  GKT+FVE R+F+ ++ SF R+W F V+  Q L I+A +D        NA    +V+S+  T  ++K  + +LD+I  + A +T       +  ++  + ++ ++ +  LY  + ++     +N   +            +  +++       L  +PA  +  E   N  + + + W  Q R YVGRGM E      KY  FW+++L  KFAF+Y F+IKPL++PTR ++ +    Y WH+       NA  +V++WAP++ +Y +D  I+Y+V   ++G L G    LGEIR+L  ++  F   P AF   L P         +      F+            A+F   WN++I + R ED ++N E++L+ MP ++  L  +++WP+FLLA+K   A  IA +     + L+ RI +DEYM YAV+ECY +L+YIL  ++  +  +  +  I   IE SI + ++ ++F++ +L  +  +   L+ +L        Q EK E   G  V A+QD++++V +D++         +  RE S +   I     E +LF     W       P    L  Q++R   LLT+KDSA ++P+NL+ARRRL FF  SLFMDMP A  V+ M+SFSV TP+Y E + YS NEL    +  +SI+FY+QKI+PDEWKNFL R+G D  +A + E  +      ELR WAS+RGQTL+RTVRGMMY R+AL LQ +L+     D E  L   +  +     L+ +  A AD+KFTYVV+CQ++G QK    P A DI  LM +  +LRVA+++  E +        YYS LVKA +NG D+EIY +KLPG P +GEGKPENQNHAIVFTRG A+QTIDMNQD+Y EEA K+RNLL+EF R+ G RPPTILG+REH+FTGSVSSLA FMS QE+SFVT+GQR+LA PL+VR HYGHPDVFDR+FHITRGGISK+SR IN+SED++AG+N+TLR+G IT++EY+QVGKGRDVGLNQI+ FE KVA GN EQ +SRD+YRLGQ FDFFRM+S YFTT+GFYF ++++V+ IYI+LYG+ YL LSG+ +T+ + A++ +  +LE AL +Q   Q+G+ + +PMV+   LE+GFL A   FI MQLQL   FFTFSLGT+THYFGRTILHGGA+Y+ TGR  VV H  FSENYRLYSRSHF+KG E+++LLVVY  + +   +      ++ S WFM+ +WL AP+LFNPSGF W+ IV D+RDW  W+  +GGIG++ ++SW++WW++E AH+R   +  R LE ILSLRFF++QYG+VY L++  +NT++ VY  SWVV+       K     +++ S    L+ RF +   F+  +  +     +  LS+ D+  S LAF+PTGWG++ IA A +P ++ T LW+  + +AR YD GMG+++F P+A  +W P +S FQTR +FN+AF+R L+I  ILAG   N
Sbjct:   56 LPATLAS-EIQRFLRIANLVESEEPRIAYLCRFHAFEIAHHMDRNSTGRGVRQFKTSLL----QRLELDEEFTVRRRKEKSDVRELKRVYHAY--------KEYIIRHGAAF--NLDNSQREKLINARRIAS--VLYEVLKTVTSGAGPQAIADRESIRAKSEFYVPYNILP--LDKGGVHQAIMHLPEIKAAVAIVRNTRGLPP-PEEF---QRHQPFLDLFEFLQYAFGFQNGNVANQREHLILLLSNTIIRQPQKQSSAPKSGDEAVDALMKKFFKNYTNWCKFLGRKNNIRLPYVKQEAL--QYKTLYIGLYLLIWGEASNLRFMPECLCYIFHHMAYELHGVLTGAVSMITGEKVAPAYGGGHESFLADVVTPIYMVVQKEAEKNKNGTADHSMWRNYDDLNEFFWSLECFEIGWPMRPEHDFFCVESSETSKPGRWRGMLRFRKQTKKTDEEIEDDEELGVLSEEQPKPTSRWLGKTNFVETRSFWQIFRSFDRMWSFFVLSLQALIIMACHDVGSPLQVFNANIFEDVMSIFITSAILKLIKGILDIIFKWKARNTMPINEKKKRLVKLGFAAMWTIILPVLYSHSRRKYICYFTNYKTWLGEWCFSPYMVAVTIYLTGSAIELVLFFVPAISKYIE-TSNHGIFKTLSWWGQPRLYVGRGMQETQVSQFKYTFFWILVLLTKFAFSYAFEIKPLIEPTRLIMKVGVRNYEWHEIFPEVKSNAAAIVAVWAPIMVVYFMDTQIWYSVYCTIFGGLYGVLHHLGEIRTLGMLRGRFHTLPSAFNASLIPHSTKDEKRRKQRGFFPFNLGRGSDGQKNSMAKFVLVWNQVINSFRTEDLISNKELDLMTMPLSSEVLSGIIRWPIFLLANKFSTALSIAKDFVGKDEVLYRRIRKDEYMYYAVKECYESLKYILQILVVGDLEKKIISGIINEIEESIRQSSLLEEFKMAELPALHDKCIELVQLLVEGSAEQLQVEKSEELHGKLVKALQDIFELVTNDMMVHGDRILDLLQSREGSGEDTGIFMRVIEPQLFESYGEWRCIHFPLPDSASLSEQIQRFLLLLTVKDSAMDIPENLDARRRLSFFATSLFMDMPDAPKVRNMMSFSVLTPHYQEDINYSTNEL-HSTKSSVSIIFYMQKIFPDEWKNFLERMGCDNLDALKKEGKEE-----ELRNWASFRGQTLSRTVRGMMYCREALKLQAFLD---MADDEDILEGYKDVERSNRPLAAQLDALADMKFTYVVSCQMFGAQKSSGDPHAQDILDLMIKYPSLRVAYVEEREEIVLDVPKKVYYSILVKA-VNGFDQEIYRVKLPGPPNIGEGKPENQNHAIVFTRGEALQTIDMNQDHYLEEAFKMRNLLQEFLRNRGRRPPTILGLREHIFTGSVSSLAWFMSYQETSFVTIGQRLLANPLRVRFHYGHPDVFDRIFHITRGGISKSSRTINLSEDVFAGYNTTLRRGCITYNEYLQVGKGRDVGLNQISKFEAKVANGNSEQTISRDIYRLGQRFDFFRMLSCYFTTIGFYFSSLISVIGIYIYLYGQLYLVLSGLQKTLILEAKVKNIKSLETALASQSFIQLGLLTGLPMVMEIGLEKGFLIAFQDFILMQLQLAAFFFTFSLGTKTHYFGRTILHGGAKYRPTGRKVVVFHANFSENYRLYSRSHFIKGFELMILLVVYELFKHTSQSNMAYSFITFSVWFMSFTWLCAPFLFNPSGFTWEIIVGDWRDWNRWIKEQGGIGIQQDKSWQSWWNDEQAHLRGSGVGARCLEIILSLRFFVYQYGLVYHLDITQSNTNIIVYALSWVVILATFFTVKAVDLGRQLFSTRKHLVFRFFKVFVFVSILTIIITLANICHLSVKDLLVSCLAFLPTGWGLILIAQAVRPKIEGTSLWEFTQVLARAYDYGMGVVLFAPMAILAWLPIISAFQTRFLFNEAFNRRLQIQPILAGKKKN 1975          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy Swiss-Prot
Match: CALSC_ARATH (Callose synthase 12 OS=Arabidopsis thaliana GN=CALS12 PE=2 SV=1)

HSP 1 Score: 1401.34 bits (3626), Expect = 0.000e+0
Identity = 802/1812 (44.26%), Postives = 1117/1812 (61.64%), Query Frame = -2

Query:  245 IPVELKRVIDSDAAMTEDLIAYNIIP---FPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSIPETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQP-VWSNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREM----DEALRQHIAQPANSCSKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFD-LSWPWRKTSFFQKPEPRSKNPLKLGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFNDGHLNAKTLREV-------------LSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFI----WFSIASVFVSFLYVRALQEESKPNSNSVVFRXXXXXXXXXXXIHFFISFLMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLHP----------------------VRXXXXXXXXXXXXXKFDAARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGKLPLVQWPLFLLASKIFLAKDIAAESRDSQDE-LWERISRDEYMKYAVQECYYALRYILTAIL--EAEGRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGILNQAEKPEHEKGAVNAVQDLYDVVRHDVLAIYLREHSDQWQSILKARTEGRLFAKLNWPRDPELKA------------------QVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEAALSRLETTDTQGYELSPEAR---------------------------ARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFIDVVETLKDGKVHTEYYSKLVKADIN-GKDKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYG-YNDXXXXXXXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIRTM--RGRILETILSLRFFLFQYGVVYKLNVQGTNTSLTVYGFSWVVLAVLIILFKVFTFSQ-KMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKK-TGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAG 5374
            +P +  R + ++A   E+   YNIIP      D P++      FPEVRAA +ALK    L + P    +      DL+D+L   FGFQKDNV NQREH+VL LAN Q R   P +    LD A V++   K L NY  WC+YL  +  +W +    +  +++L+V LY LIWGEAAN+RF+PEC+CYIFH+MA E+    ++ L ++  QP    S  G  +FL  V+ P+YD + AE   ++NG   H  WRNYDD NEYFW+  CF  L WP    S F K             G+  GKT FVE RTFF+LY SF RLW+ L +  Q   I+A+ +    +   R++             L++  T+  M+  ++VLD    Y   S   +    R+ ++ I    W    +V  + ++ +  Q+    N+ +     +L  +G +         L  IP      E   N  +   + W  Q + +VGRG+ E   D IKY  FW+ +L+ KF F+YF Q+KP++KP++ +  + +++Y WH F    + N  +V  LW PV+ +YL+DI I+Y + S++ G ++G  D LGEIR +  ++  F+ F  A    L P                      +R       + +E ++ +A +F+  WNEII   REED +++ E+ELL +PKN+  + +++WP FLL +++ LA   A E  D+ D+ LW +I ++EY + AV E Y +++++L +I+  + E  + +   ++ I  SI  +  +  F+++ L  +   +  L+G++N  E        VN +Q LY++                 Q  ++ +T  +L  +   PRDP  K                   QV+RL+++LT +DS  +VP NLEARRR+ FF+NSLFM+MP A  V++M++FSV TPYYSE V+YS  +L  + EDGIS L+YLQ IY DEWKNF  R+ R+    ++EL+   + + +LR WASYRGQTLARTVRGMMYY +AL +  +L+  +  D       L +      EL  ++                              A +KFTYVV CQIYG QK +++P+A +I  LM++NEALR+A++D V     G+  T+YYS LVK D    K+ EI+ +KLPG  KLGEGKPENQNHA++FTRG+AVQTIDMNQD+YFEEALK+RNLL+E++  HGIR PTILGVREH+FTGSVSSLA FMS QE+SFVTLGQRVLA PLKVRMHYGHPDVFDR + ++RGGISKASR+INISEDI+AGFN TLR GN+THHEYIQVGKGRDVGLNQI++FE KVA GNGEQVLSRDVYRLG   DFFRM+SF++TTVGF+F TM+ +LT+Y FL+GR YLALSGV ++    +  T NAAL   LN QF+ Q+G+F+A+PM++ + LE+GFL AI +FI MQ+QL  VF+TFS+GTR HYFGRTILHGGA+Y+ATGRGFVV H  F+ENYRLY+RSHFVK +E+ L+L+VY ++      +L YI ++I+SWF+ +SW+ AP++FNPSGF+W K V DF D+ NW++Y+G I  K E+SWE WW EE  H+R     G  +E IL LRFF FQYG+VY+L +   +TSL VY FSW+ +  + +LF V  +++ K S    +  R +Q +  ++AI  +   +  T  S  DIF S+LAF+PTGWGIL IA   + ++K  T  W +V S+AR+YD   G++I VPVAF SW P   + QTR++FN+AFSRGL I  I+ G
Sbjct:    8 VPPQTGRPLAAEAVGIEEE-PYNIIPVNNLLADHPSL-----RFPEVRAAAAALKTVGDLRRPPY---VQWRSHYDLLDWLALFFGFQKDNVRNQREHMVLHLANAQMRLSPPPDNIDSLDSAVVRRFRRKLLANYSSWCSYLGKKSNIWISDRNPDSRRELLYVGLYLLIWGEAANLRFMPECICYIFHNMASELNKILEDCLDENTGQPYLP-SLSGENAFLTGVVKPIYDTIQAEIDESKNGTVAHCKWRNYDDINEYFWTDRCFSKLKWPLDLGSNFFKSR-----------GKSVGKTGFVERRTFFYLYRSFDRLWVMLALFLQAAIIVAWEEKPDTSSVTRQLWNALKARDVQVRLLTVFLTWSGMRLLQAVLDAASQYPLVSRETKRHFFRMLMKVIAAAVWIVAFTVLYTNIWKQKRQDRQWSNAATTKIYQFLYAVGAFLVPEILALALFIIPWMRNFLEET-NWKIFFALTWWFQGKSFVGRGLREGLVDNIKYSTFWIFVLATKFTFSYFLQVKPMIKPSKLLWNLKDVDYEWHQFYG--DSNRFSVALLWLPVVLIYLMDIQIWYAIYSSIVGAVVGLFDHLGEIRDMGQLRLRFQFFASAIQFNLMPEEQLLNARGFGNKFKDGIHRLKLRYGFGRPFKKLESNQVEANKFALIWNEIILAFREEDIVSDREVELLELPKNSWDVTVIRWPCFLLCNELLLALSQARELIDAPDKWLWHKICKNEYRRCAVVEAYDSIKHLLLSIIKVDTEEHSIITVFFQIINQSIQSEQFTKTFRVDLLPKIYETLQKLVGLVNDEETDSGR--VVNVLQSLYEIATR--------------QFFIEKKTTEQLSNEGLTPRDPASKLLFQNAIRLPDASNEDFYRQVRRLHTILTSRDSMHSVPVNLEARRRIAFFSNSLFMNMPHAPQVEKMMAFSVLTPYYSEEVVYSKEQLRNETEDGISTLYYLQTIYADEWKNFKERMHREGIKTDSELW--TTKLRDLRLWASYRGQTLARTVRGMMYYYRALKMLAFLDSASEMDIREGAQELGSVRNLQGELGGQSDGFVSENDRSSLSRASSSVSTLYKGHEYGTALMKFTYVVACQIYGSQKAKKEPQAEEILYLMKQNEALRIAYVDEVPA---GRGETDYYSVLVKYDHQLEKEVEIFRVKLPGPVKLGEGKPENQNHAMIFTRGDAVQTIDMNQDSYFEEALKMRNLLQEYNHYHGIRKPTILGVREHIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRFWFLSRGGISKASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQISMFEAKVASGNGEQVLSRDVYRLGHRLDFFRMLSFFYTTVGFFFNTMMVILTVYAFLWGRVYLALSGVEKSALADSTDT-NAALGVILNQQFIIQLGLFTALPMIVEWSLEEGFLLAIWNFIRMQIQLSAVFYTFSMGTRAHYFGRTILHGGAKYRATGRGFVVEHKGFTENYRLYARSHFVKAIELGLILIVYASHSPIAKDSLIYIAMTITSWFLVISWIMAPFVFNPSGFDWLKTVYDFEDFMNWIWYQGRISTKSEQSWEKWWYEEQDHLRNTGKAGLFVEIILVLRFFFFQYGIVYQLKIANGSTSLFVYLFSWIYIFAIFVLFLVIQYARDKYSAKAHIRYRLVQFLLIVLAILVIVALLEFTHFSFIDIFTSLLAFIPTGWGILLIAQTQRKWLKNYTIFWNAVVSVARMYDILFGILIMVPVAFLSWMPGFQSMQTRILFNEAFSRGLRIMQIVTG 1773          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy TrEMBL
Match: A0A061GRD6_THECC (Glucan synthase-like 10 isoform 2 OS=Theobroma cacao GN=TCM_040336 PE=4 SV=1)

HSP 1 Score: 2930.58 bits (7596), Expect = 0.000e+0
Identity = 1439/1601 (89.88%), Postives = 1512/1601 (94.44%), Query Frame = -2

Query: 1079 FGMGSVGHAAGGIA----GYVPSSL-NNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDVARLLEFYRLYREKNNVDKLREEEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQLTEEIPVELKRVIDSDAAMTEDLIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSIPETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQPVWSNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIAQPANSCSKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWRKTS-FFQKPEPRSKNPLKLGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFNDGHLNAKTLREVLSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIASVFVSFLYVRALQEESKPNSNSVVFRXXXXXXXXXXXIHFFISFLMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLHPVRXXXXXXXXXXXXXKFDAARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGKLPLVQWPLFLLASKIFLAKDIAAESR-DSQDELWERISRDEYMKYAVQECYYALRYILTAILEAEGRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGILNQAEKPEHEKGAVNAVQDLYDVVRHDVLAIYLREHSDQWQSILKARTEGRLFAKLNWPRDPELKAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEAALSRLETTDTQGYELSPEARARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFIDVVETLKDGKVHTEYYSKLVKADINGKDKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHF 5860
            FGM ++G   GGIA    GYVPSSL  NRDID ILR ADEIQD++PNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQD+ARL EFY+LYREKNNVDKLREEEM LRESGVFS NLGELE+KTLKRK+VFGTLRVLGMVLEQLTEEIP ELKRVIDSDAAMTEDLIAYNIIP  LDAPTIT+AI SFPEVRAAVS LK++  LP+LP+DFSIP+TRS DL+DFLHYVFGFQKDNVSNQREH+VLLLANEQSR GIPEE EPKLDEAAVQKVFLKSL NYI+WCNYLCIQPVWSNLDAV++EKK+LFVSLYFLIWGEAANIRFLPECLCYIFHHM REMDE LRQ +AQPANSC  +  VSFLDQVITPL++VVAAEAANN NGRAPHSAWRNYDDFNEYFWSLHCF+LSWPWRK+S FFQKP+PRSKNPLK GGGQHRGKTSFVEHRTFFHLYHSFHRLWIFL MMFQGLTIIAFNDGHLN+KTLREVLSLGPTFVVMKF ESVLDV MMYGAYSTTRRLAVSRI LRF+WFS+ASV +SFLYV+ALQEESKPNS+SVVFRLYLIVIGIY GI FFISFLMRIPACHRLT  CD  SLIRFIKWMRQERYYVG GMYERTTDFIKYM+FWLIILSGKF+FAYFFQIKPLVKPTRT++ MD I+YSWHDFVS+NNHNALTV +LWAPVIAMYLLDIY+FYTVLSAVWGFLLGARDRLGEIRSL AVQKLFEEFP AFMK LHPVR S SS+++VVEK+KFDAARFSP WNEIIKNLREEDYLTN EMELL MPKNTG LPLVQWPLFLLASKIFLA + AAE   DSQDELWERISRD++MKYAVQECY+ALR+ILT ILEAEGR WVERIYEGIEASI KK+I  DFQLNKLQLVISRVTALLGILNQAEKPEHEKGAV AVQDLYDVVRHDVLAI +REH +QW +I KARTEGRLFA L WPRDPELKAQVKRLYSLLTIKDSASNVPKNLEA RRLEFFTNSLFMDMPP RPV EMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDEN+AETEL+DSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLER+N+ DTEAALSRL+TTDTQG+ELSPEARARADLKFTYVVTCQIYG+QKEEQKPEAADIALLMQRNEALRVAFIDVVE LKDG VHTEY+SKLVKADINGKDKEIYAIKLPG+PKLGEGKPENQNHAIVFTRGNA+QTIDMNQDNYFEEALK+RNLLEEF RDHGIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLA PLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVG+YFCTMLTVLT+Y FLYG+AYLALSGVGETMQ RA+ITDN ALE ALNTQFLFQIGIFSAVPM+LGFILEQGFLRA+VSF+TMQ+QLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHF
Sbjct:   21 FGMRTIGQPVGGIASGIAGYVPSSLAKNRDIDAILRAADEIQDDDPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDIARLQEFYKLYREKNNVDKLREEEMKLRESGVFSSNLGELEQKTLKRKKVFGTLRVLGMVLEQLTEEIPEELKRVIDSDAAMTEDLIAYNIIP--LDAPTITDAIASFPEVRAAVSELKYFRGLPRLPADFSIPDTRSADLLDFLHYVFGFQKDNVSNQREHIVLLLANEQSRLGIPEETEPKLDEAAVQKVFLKSLKNYIEWCNYLCIQPVWSNLDAVSREKKLLFVSLYFLIWGEAANIRFLPECLCYIFHHMVREMDEILRQQMAQPANSCCSESGVSFLDQVITPLFEVVAAEAANNGNGRAPHSAWRNYDDFNEYFWSLHCFELSWPWRKSSSFFQKPKPRSKNPLKSGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLAMMFQGLTIIAFNDGHLNSKTLREVLSLGPTFVVMKFIESVLDVFMMYGAYSTTRRLAVSRILLRFVWFSVASVVISFLYVKALQEESKPNSDSVVFRLYLIVIGIYAGIQFFISFLMRIPACHRLTNQCDRWSLIRFIKWMRQERYYVGLGMYERTTDFIKYMVFWLIILSGKFSFAYFFQIKPLVKPTRTIVTMDAIQYSWHDFVSKNNHNALTVATLWAPVIAMYLLDIYLFYTVLSAVWGFLLGARDRLGEIRSLGAVQKLFEEFPAAFMKTLHPVRTSTSSTNQVVEKNKFDAARFSPVWNEIIKNLREEDYLTNLEMELLLMPKNTGSLPLVQWPLFLLASKIFLANNCAAERIIDSQDELWERISRDDHMKYAVQECYHALRFILTEILEAEGRMWVERIYEGIEASIEKKSIHVDFQLNKLQLVISRVTALLGILNQAEKPEHEKGAVKAVQDLYDVVRHDVLAINMREHYEQWNNISKARTEGRLFANLKWPRDPELKAQVKRLYSLLTIKDSASNVPKNLEAGRRLEFFTNSLFMDMPPPRPVHEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENSAETELFDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERDNSGDTEAALSRLDTTDTQGFELSPEARARADLKFTYVVTCQIYGKQKEEQKPEAADIALLMQRNEALRVAFIDVVEILKDGNVHTEYFSKLVKADINGKDKEIYAIKLPGNPKLGEGKPENQNHAIVFTRGNAIQTIDMNQDNYFEEALKMRNLLEEFHRDHGIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLANPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGYYFCTMLTVLTVYFFLYGKAYLALSGVGETMQDRAQITDNTALETALNTQFLFQIGIFSAVPMILGFILEQGFLRAVVSFVTMQIQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHF 1619          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy TrEMBL
Match: A0A022PT59_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a000080mg PE=4 SV=1)

HSP 1 Score: 2928.27 bits (7590), Expect = 0.000e+0
Identity = 1452/1891 (76.78%), Postives = 1649/1891 (87.20%), Query Frame = -2

Query:  224 GHAAGGIAGYVPSSLNNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDVARLLEFYRLYREKNNVDKLREEEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQLTEE---------IPVELKRVIDSDAAMTEDLIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSIPETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQPVWSNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIAQPANSCSKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWRKTS-FFQKPEPRSKNPLKLGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFNDGHLNAKTLREVLSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIASVFVSFLYVRALQEESKPNSNSVVFRXXXXXXXXXXXIHFFISFLMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLH---PVRXXXXXXXXXXXXXKFDAARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGKLPLVQWPLFLLASKIFLAKDIAAESRDSQDELWERISRDEYMKYAVQECYYALRYILTAILE----AEGRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGILNQAEKPEHEKGAVNAVQDLYDVVRHDVLAIYLREHSDQWQSILKARTEGRLFAKLNWPRDPELKAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEAALSRLETTDTQGYELSPEARARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFIDVVETLKDGKVHTEYYSKLVKADINGKDKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYGYND-XXXXXXXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIRTMRGRILETILSLRFFLFQYGVVYKLNVQGTNTSLTVYGFSWVVLAVLIILFKVFTFSQKMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGNNPNTGL 5842
            G    G+A  VPSSL NR+ID ILR ADEIQD++PNV+RILCEHAYSL+QNLDPNSEGRGVLQFKTGLMSVIKQKLAKRE G IDRSQD+ARL EFY+LYRE NNVDKLREEE+ LRESGVFSGNLGELERKT+KRKRV  TL+VLG VLEQL+++         IP ELKRV++SDAAMTEDLI YNIIP  LD P ITN IVSFPEVRAA S+LK++  LPKLP+ FS+P +RS D+ DFL Y FGFQKDN+SNQRE VV LLANEQSR  I EE EP LDEAAVQKVFLKSLDNYIKWCNYL I PVWSNLDAV+KEKK+LF+SLYFLIWGEAAN+RFLPECLCYIFHHMARE++E LR+ +AQPA+SC  +  VSF+DQVI PLYDV+ AEA NN NG APHSAWRNYDDFNEYFWSLHCF+LSWPWRK+S FF KP PRSKN LK  GG+  GKTSFVEHRTF HLYHSFHRLWIFL MMFQGLT+IAFN+G LN KT+RE+LS+GPT+ VMKF +SVLD+IMMYGAYST+RRLAV+R+FLRF+ +S+ASV + FLY RAL+E+S  N+NS+ ++LY+I+I  Y G  FF+SFL  IPACHRL++  D+  L+RF+KWM Q                                      I+PLV PTR ++ +    YSWHDFVS+NNHNA+TV SLW PV+A+YLLDI+IFYTV+SAVWGFLLGARDRLGEIRSLDAV +LFE+FP AFM  LH   P R S  SSS+ +EK+K DAARF+PFWNEIIKNLREEDY++N EMELL MPKN+G L LVQWPLFLLASK+FLAKDIA E++DSQ+ELW+RISRD+YMKYAV+EC+Y++++ILTAIL+     EG+ WVERIYE I+ SI    I  DF+LNKL LVI +VTALLGIL +   PE E GAV A+ DLYDV+RHD+L+I +R++ + W  + KARTEGRLF KL WP+D ELKAQV RLYSLLTIKDSA+NVPKNLEARRRLEFFTNSLFM+MP A+PV+EMLSF VFTPYYSEIVLY M++LLKKNEDGIS LFYLQKIYPDEWKNFLARIGRDENA+E+EL D+P+ ILELRFWASYRGQTLARTVRGMMYYRKALMLQ YLER +A D EA ++  ++TD QG+ELSPEARA+ADLKFTYVVTCQIYG+Q+EEQKPEAADIALLMQRNEALRVAFIDVVETLKDGKVH+EY+SKLVKADINGKDKE+Y+IKLPG+PKLGEGKPENQNHA+VFTRGNA+QTIDMNQDNYFEEALK+RNLLEEF  DHG+RPPTILGVREHVFTGSVSSLASFMSNQE+SFVTLGQRVL+ PLKVRMHYGHPDVFDRVFHITRGGISK+SRIINISEDI++GFNSTLRQGN+THHEYIQVGKGRDVGLNQIA+FEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVG+YFCTMLTVLT+Y FLYGR YLALSGVGE++Q  A +  N AL AALN QFLFQIG+F+AVPM+LGFILEQGFLRA+VSF+TMQ QLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVV+HIKF+ENYRLY+RSHFVKG+E+ LLL+VYLAYGYN+  AL+YILL++SSWF+A SWLFAPYLFNPSGFEWQK VEDFRDWTNWL YRGGIGVKG ESWEAWWDEE++HIRT  GR++ETILSLRFF+FQYG+VYKL+VQGT+TSLTVYGFSW+VLAVLIILFKVFTFSQK+SVNFQLLLRFIQGVSF+ A+AG+ VAVA+T+L+I DIFA ILAF+PTGWGIL IA AWKP +KK GLWKS+RS+ARLYDA MG++IF+P+A  SWFPFVSTFQTRLM+NQAFSRGLEISLILAGNNPNTG+
Sbjct:   27 GRPETGLATNVPSSLGNRNIDDILRAADEIQDDDPNVSRILCEHAYSLSQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREGGGIDRSQDIARLREFYKLYRETNNVDKLREEEIKLRESGVFSGNLGELERKTVKRKRVLATLKVLGNVLEQLSKDVSPEEAERLIPEELKRVMESDAAMTEDLIPYNIIP--LDGPNITNPIVSFPEVRAAASSLKYFRGLPKLPATFSVPASRSLDIFDFLQYTFGFQKDNISNQREDVVHLLANEQSRLRILEELEPILDEAAVQKVFLKSLDNYIKWCNYLGILPVWSNLDAVSKEKKLLFISLYFLIWGEAANVRFLPECLCYIFHHMARELEEILREQVAQPADSCVSESGVSFIDQVIRPLYDVICAEAGNNNNGAAPHSAWRNYDDFNEYFWSLHCFELSWPWRKSSIFFLKPTPRSKNFLKSSGGKRCGKTSFVEHRTFLHLYHSFHRLWIFLAMMFQGLTLIAFNNGQLNTKTMREILSVGPTYFVMKFFKSVLDIIMMYGAYSTSRRLAVTRVFLRFLSYSLASVVICFLYARALEEQSNANNNSITYKLYVIIISSYAGAKFFLSFLQHIPACHRLSDRGDSWRLVRFMKWMHQ--------------------------------------IRPLVGPTRLIVNIPVTRYSWHDFVSKNNHNAMTVASLWTPVVAIYLLDIHIFYTVISAVWGFLLGARDRLGEIRSLDAVHQLFEKFPAAFMNNLHVPLPNRDSLHSSSQSLEKNKIDAARFAPFWNEIIKNLREEDYISNLEMELLQMPKNSGSLQLVQWPLFLLASKLFLAKDIAVENKDSQEELWDRISRDDYMKYAVEECFYSVKFILTAILDDEGNNEGKKWVERIYEDIQGSIANGCIHVDFRLNKLPLVIQKVTALLGILKKDHTPELETGAVKAILDLYDVMRHDILSINMRDNYETWNMLSKARTEGRLFQKLKWPQDAELKAQVGRLYSLLTIKDSAANVPKNLEARRRLEFFTNSLFMEMPEAKPVREMLSFCVFTPYYSEIVLYRMSDLLKKNEDGISTLFYLQKIYPDEWKNFLARIGRDENASESELSDNPNHILELRFWASYRGQTLARTVRGMMYYRKALMLQAYLERMSAGDVEAGITGKDSTDVQGFELSPEARAQADLKFTYVVTCQIYGKQREEQKPEAADIALLMQRNEALRVAFIDVVETLKDGKVHSEYFSKLVKADINGKDKEVYSIKLPGNPKLGEGKPENQNHAVVFTRGNAMQTIDMNQDNYFEEALKMRNLLEEFHCDHGLRPPTILGVREHVFTGSVSSLASFMSNQEASFVTLGQRVLSNPLKVRMHYGHPDVFDRVFHITRGGISKSSRIINISEDIFSGFNSTLRQGNVTHHEYIQVGKGRDVGLNQIAVFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGYYFCTMLTVLTVYAFLYGRVYLALSGVGESIQDIADVLGNTALSAALNAQFLFQIGVFTAVPMILGFILEQGFLRAVVSFVTMQFQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVQHIKFTENYRLYARSHFVKGMEICLLLIVYLAYGYNEGGALAYILLTVSSWFLAGSWLFAPYLFNPSGFEWQKTVEDFRDWTNWLLYRGGIGVKGGESWEAWWDEELSHIRTFSGRVMETILSLRFFIFQYGIVYKLDVQGTDTSLTVYGFSWIVLAVLIILFKVFTFSQKISVNFQLLLRFIQGVSFLFALAGIVVAVAITNLTIADIFACILAFLPTGWGILCIACAWKPVMKKVGLWKSIRSIARLYDAAMGILIFIPIALCSWFPFVSTFQTRLMYNQAFSRGLEISLILAGNNPNTGI 1877          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy TrEMBL
Match: M4ELG0_BRARP (Uncharacterized protein OS=Brassica rapa subsp. pekinensis GN=Bra029628 PE=4 SV=1)

HSP 1 Score: 2924.04 bits (7579), Expect = 0.000e+0
Identity = 1457/1883 (77.38%), Postives = 1667/1883 (88.53%), Query Frame = -2

Query:  224 YVPSSL-NNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDVARLLEFYRLYREKNNVDKLREEEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQLTEEIPVELKRVIDSDAAMTEDLIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSIPETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQPVWSNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIAQPANSC-------SKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWR-KTSFFQKPEPRSKNPLKLGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQ----GLTIIAFNDGHL-NAKTLREVLSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIASVFVSFLYVRALQEESKPNSNSVVFRXXXXXXXXXXXIHFFISFLMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLH-PV-RXXXXXXXXXXXXXKFDAARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGKLPLVQWPLFLLASKIFLAKDIAAESRDSQDELWERISRDEYMKYAVQECYYALRYILTAILEAEGRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGILNQAEKPEHEKGAVNAVQDLYDVVRHDVLAIYLREHSDQWQSILKARTEGRLFAKLNWPRDPELKAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEAALSRLETTDTQGYELSPEARARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFIDVVETLKDGKVHTEYYSKLVKADINGKDKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFD--RDHGIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYGYNDX-XXXXXXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIRTMRGRILETILSLRFFLFQYGVVYKLNVQGTNTSLTVYGFSWVVLAVLIILFKVFTFSQKMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGNNPNTGL 5815
            YVPSSL NNRDID ILR ADE+QDE+P++ARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSV+KQKLAKREVGTIDRSQD+ RL +FYRLYREKNNVD L+E+E  LRESGVF+    E+ERKTL+RKRVF TL+VLG VLEQ+ +EIP ELK VIDSDAAM+ED IAYNIIP  LDAP  TNA  SFPEV+AAV+ALK++  LPKLP+DF IP TR+ D++DFLHY+FGFQKD+VSNQREH+VLLLANEQSR  IPEE EPKLD+AAV+KVFLKSLDNYIKWC+YLCIQP WSNL+ ++ EK++LF+SLYFLIWGEAANIRFLPECLCYIFHHM REMDE LRQ +A+PA SC       S DGV SFLD VI PLY VV+AEA NN+NGRAPHSAWRNYDDFNEYFWSLH F+L WPWR  +SFFQKP PR K  L+ G  +HRGKTSFVEHRTF HLYHSFHRLWIFL MMFQ     L IIAFN   L + KTLRE+LSLGPTFVVMKF+ESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFS+ASV VSFLYV+ALQE+S PNSNSV+F+ Y+I+I IYGG+ FF+S LMR P CH +   CD   +IRF KWMRQER+YVGRGMYE+T+DFIKY++FW+++LS KF+FAYF QIKPLV PTR ++  DNI+YSWHD VSRNN+NALTV SLWAPV+A+YLLDI+IFYT++SA  GFLLGARDRLGEIRSL+A+ K FEEFP AFM+ LH P+   ++  S +  +K+K DAA F+PFWN+IIK LREEDY+T+FEM+LL MPKN+G+L LVQWPLFLL+SKI LAK+IAAES ++Q+E+ ERI RD+YMKYAV+E Y+ L+ +L   LEAEGR WVERIYE I+ASI  + I  DFQLNKL  VI+RVTALLGIL + E PEH KGA+ A+QDLYDV+R D+L   +R   + W  + +A  EGRLF KL WP+DPELKA VKRLYSL TIKDSA++VP+NLEARRRL+FFTNSLFMD+PP + V +MLSFSVFTPYYSE+VLYSM EL K+NEDGISILFYLQKIYPDEWKNFLARIG+DENA E +L +   DILELRFWASYRGQTLARTVRGMMYYRKALMLQ+YLER+  RD E+     + TD++G+ELSPEARA+ADLKFTYVVTCQIYGRQKE+QKPEAADIALLMQRNEALR+A+IDVV+T K+GK HTEYYSKLVKADI+GKDKEIY+I+LPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALK+RNLLEEFD  R HGIRPPTILGVREHVFTGSVSSLASFMSNQE+SFVTLGQRVLA PLK+RMHYGHPDVFDRVFHITRGGISKASR+INISEDI+AGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQL DFFRMMSFYFTTVGFYFCTMLTVLT+YIFLYGR YLALSGVG T++ RA + D+ AL AALN QFLFQIG+F+AVPM+LGFILEQGFL+AIVSF TMQ QLCT+FFTFSLGTRTHYFGRTILHGGARYQATGRGFVV+HIKFSENYRLYSRSHF+K +EV+LLLVVYL+YG +++ A+SYILL++SSWF+A+SWLFAPYLFNP+GFEWQK+VEDF++WTNWLFYRGGIGVKG ESWEAWW+EE++HIRT+ GRI+ETILSLRFF+FQYG+VYKLN+QG++TS+ VYG+SW   A+LI+LFKVFTFSQK+SVNFQL+LRF+QGVS ++A+AG+ VA+ LT+LS+ DIFASILAF+PTGWGILSIA AWKP +K+ G+WKSVRS+ARL+DAGMGM+IF+PVA  SWFPFVSTFQTR+MFNQAFSRGLEISLILAGNNPN+GL
Sbjct:   39 YVPSSLANNRDIDAILRAADELQDEDPSIARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVVKQKLAKREVGTIDRSQDIKRLQDFYRLYREKNNVDTLKEDEKQLRESGVFTK---EMERKTLRRKRVFATLKVLGNVLEQVAKEIPDELKHVIDSDAAMSEDTIAYNIIP--LDAPVTTNATTSFPEVQAAVAALKYFPGLPKLPADFPIPATRNADMLDFLHYIFGFQKDSVSNQREHIVLLLANEQSRLTIPEEREPKLDDAAVRKVFLKSLDNYIKWCDYLCIQPAWSNLETISGEKRLLFLSLYFLIWGEAANIRFLPECLCYIFHHMVREMDEILRQRVARPAESCMPVESRGSDDGV-SFLDHVIAPLYGVVSAEAFNNDNGRAPHSAWRNYDDFNEYFWSLHSFELGWPWRTSSSFFQKPIPRKKYDLQTGRAKHRGKTSFVEHRTFLHLYHSFHRLWIFLAMMFQTSLQALAIIAFNKNDLYSRKTLREILSLGPTFVVMKFSESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSLASVVVSFLYVKALQEDSNPNSNSVMFKFYVILIAIYGGVQFFLSILMRFPTCHNIANKCDRWPVIRFFKWMRQERHYVGRGMYEKTSDFIKYLLFWVVVLSAKFSFAYFLQIKPLVGPTRMIVKQDNIQYSWHDLVSRNNYNALTVASLWAPVVAIYLLDIHIFYTLVSAFLGFLLGARDRLGEIRSLEAIHKQFEEFPGAFMRALHVPITNRTSDPSHQAADKNKVDAAHFAPFWNQIIKCLREEDYITDFEMDLLLMPKNSGRLQLVQWPLFLLSSKILLAKEIAAES-NTQEEIIERIERDDYMKYAVEEVYHTLKLVLMETLEAEGRMWVERIYEDIQASIKDRKIHHDFQLNKLSRVITRVTALLGILKENETPEHAKGAIKALQDLYDVMRLDILTFNMRGQYETWNILTQAWNEGRLFTKLKWPKDPELKALVKRLYSLFTIKDSAAHVPRNLEARRRLQFFTNSLFMDVPPPKSVDKMLSFSVFTPYYSEVVLYSMAELTKRNEDGISILFYLQKIYPDEWKNFLARIGQDENALEGDLRNE-RDILELRFWASYRGQTLARTVRGMMYYRKALMLQSYLERKAGRDGESTPFGNDATDSEGFELSPEARAQADLKFTYVVTCQIYGRQKEDQKPEAADIALLMQRNEALRIAYIDVVDTPKEGKSHTEYYSKLVKADISGKDKEIYSIRLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKMRNLLEEFDPNRGHGIRPPTILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLAKPLKIRMHYGHPDVFDRVFHITRGGISKASRVINISEDIFAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLLDFFRMMSFYFTTVGFYFCTMLTVLTVYIFLYGRLYLALSGVGATIRERAILLDDTALSAALNAQFLFQIGVFTAVPMILGFILEQGFLQAIVSFTTMQFQLCTIFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVKHIKFSENYRLYSRSHFIKAMEVILLLVVYLSYGNDEAGAVSYILLTVSSWFLAVSWLFAPYLFNPAGFEWQKVVEDFKEWTNWLFYRGGIGVKGAESWEAWWEEEISHIRTLSGRIVETILSLRFFIFQYGIVYKLNLQGSDTSIAVYGWSWAAFAMLIVLFKVFTFSQKVSVNFQLVLRFVQGVSLLVALAGIVVAIVLTNLSVTDIFASILAFIPTGWGILSIACAWKPVIKRLGMWKSVRSLARLFDAGMGMLIFLPVALCSWFPFVSTFQTRMMFNQAFSRGLEISLILAGNNPNSGL 1913          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy TrEMBL
Match: W9QD66_9ROSA (Callose synthase 9 OS=Morus notabilis GN=L484_018426 PE=4 SV=1)

HSP 1 Score: 2894.38 bits (7502), Expect = 0.000e+0
Identity = 1468/1912 (76.78%), Postives = 1619/1912 (84.68%), Query Frame = -2

Query:  224 GMGSVGHAAGGIAGYVPSSL-NNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDVARLLEFYRLYREKNNVDKLREEEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQLTEEIPVELKRVIDSDAAMTEDLIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSIPETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQPVWSNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIAQPANSCSKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWRKTSFFQKPEPRSKNPLKLGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFNDGHLNAKTLREVLSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIASVFVSFLYVRALQEESKPNSNSVVFRXXXXXXXXXXXIHFFISFLMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLHPVRXXXXXXXXXXXXXKFDAARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGKLPLVQWPLFLLASKIFLAKDIAAESRDSQDELWERISRDEYMKYAVQECYYALRYILTAILEAEGRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGILNQAEKPEHEKGAVNAVQDLYDVVRHDVLAIYLREHSDQWQSILKARTEGRLFAKLNWPRDPEL----------------------------KAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENA----RDTEAALSRLETTDTQGYELSPEARARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFIDVVETLKDGKVHTEYYSKLVKADINGKDKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYGYNDX-XXXXXXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIRTMRGRILETILSLRFFLFQYGVVYKLNVQGTNTSLTVYGFSWVVLAVLIILFKVFTFSQKMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGNNPNTGL 5857
            G  + G    GIAGYVPSSL NNRDID ILR ADEIQDE+PNV+RILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKRE G IDRSQD+ARL EFY+LYREKN+VDKLREEEM L+ESG FSGNLGELERK +KRKRVF TL+VLG VLEQL+ EIP ELKRV++SDAAMTEDLIAYNIIP  LDAP+ TNAIVS PEVRAA+ ALK++  LP LP+DFSIP TR  D++DFLHY+FGFQKD+VSNQREH+V LLANEQSR  I EE EP+LDEAAVQ VFLKSL+NYIKWC+YL IQPVWSN + V+KEKK+LFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDE LRQ IAQPANSC+ +  VSFLDQVI PLYDVV                                                   +   LK GG +H+GKTSFVEHRTF HLYHSFHRLWIFL MMFQGL IIAFN G+LNAKT REVLSLGPTF VMKF ES+                                        +ALQEESK N N V+FRLY+I++GIY GI FFISFL+RIPACH+LT  CD  S+IRF+KWMRQE YYVGRGMYERTTDFIKYM+FWL++L  KF+FAYF QIKPLV PT+T++ MD+IEYSWH  VS+NN+N  TVVSLWAPV+A+Y+LDI++FYTV SA+ GFLLGARDRLGEIRSL+A+ KLFE+              S  SSSEVVEK K DAARFSPFWNEIIKNLREEDY+T  EMELL MPKN+G LPLVQWPLFLLASKIFLAKDIA ESRDSQ+ELWERISRD+YMKYAVQEC++ +R ILT IL+ EG+ WVERIYE I ASI K++I  DFQLNKL LVISRV AL+GIL + E  + EKGAV AVQDLYDV+RHD L+I + ++ + W  + KARTEGRLF K+ WP+D EL                            ++QVKRL+SLLTIKDSA+NVPKNLEARRRL+FFTNSLFMD+P A+PV EMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKI+PDEWKNFLARIGR ENA E+EL DSPSDILELRFWASYR QTLARTVRGMMYYRKALMLQTYLER N+     D EAA+S  + TDTQG+ELSP+ARA+ADLKFTYVVTCQIYG+QKE++KPEAADIALLMQRNEALRVAFID VE+L +GKVHTEYYSKLVK DINGKDKEIY+IKLPG+PKLGEGKPENQNHAI+FTRGNA+QTIDMNQDNYFEEALK+RNLLEEF RDHGIRPPTILGVREHVFTGSVSSLASFMSNQE+SFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDI+AGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVG+YFCTMLTVLT+YIFLYG+ YLALSGVGET+Q+RARI DN AL  ALNTQFLFQIGIF+AVPMVLGFILEQGFLRA+VSF+TMQLQLC+VFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFVKGLEVVLLLVVYLAYGYN+S A+ YILLSISSWFM+LSWLFAPYLFNPSGFEWQK VEDFRDWTNWL YRGGIGVKG ESWEAWWDEE++HIRT+ GRI+ETILSLRFF+FQYGVVYKL+VQG++ SLTVYG SW+VLAVLIILFKVFTFSQK+SVNFQL+LRF+QGVSF++A+AG+AVA+ LTDL++ DIFA ILAFVPTGWGILSIA AWKP +KK GLWKS+RS+ARLYDAGMGM+IFVPVA  SWFPFVSTFQTRLM+NQAFSRGLEISLILAGNN N+G+
Sbjct:   22 GKDAYGRPVEGIAGYVPSSLANNRDIDEILRAADEIQDEDPNVSRILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREGGLIDRSQDIARLQEFYKLYREKNDVDKLREEEMKLKESGAFSGNLGELERKRVKRKRVFATLKVLGAVLEQLSPEIPDELKRVMESDAAMTEDLIAYNIIP--LDAPSTTNAIVSLPEVRAAILALKYFRGLPTLPTDFSIPATRKADMLDFLHYMFGFQKDSVSNQREHIVQLLANEQSRLHILEESEPELDEAAVQSVFLKSLENYIKWCSYLGIQPVWSNFEVVSKEKKLLFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEILRQQIAQPANSCNSENGVSFLDQVIFPLYDVV---------------------------------------------------AAGMLKSGGSKHQGKTSFVEHRTFLHLYHSFHRLWIFLAMMFQGLAIIAFNKGNLNAKTFREVLSLGPTFAVMKFFESM----------------------------------------KALQEESKRNGNPVIFRLYVILVGIYAGIQFFISFLLRIPACHQLTNQCDRWSIIRFVKWMRQEHYYVGRGMYERTTDFIKYMLFWLVVLGAKFSFAYFLQIKPLVGPTQTIVKMDSIEYSWHSIVSKNNYNFWTVVSLWAPVVAIYILDIHVFYTVTSAICGFLLGARDRLGEIRSLEALHKLFEQ-------------TSHQSSSEVVEKKKVDAARFSPFWNEIIKNLREEDYITTHEMELLEMPKNSGTLPLVQWPLFLLASKIFLAKDIAVESRDSQEELWERISRDDYMKYAVQECFHTVRLILTNILDDEGKMWVERIYEDIYASIAKRSIHVDFQLNKLALVISRVFALMGILKEGESSDMEKGAVKAVQDLYDVIRHDFLSIDMSKNYETWNLLSKARTEGRLFTKIKWPKDTELVCSGSFSCFSIYLLVISPRDVVIKEDVQRSQVKRLHSLLTIKDSAANVPKNLEARRRLQFFTNSLFMDIPMAKPVNEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIFPDEWKNFLARIGRHENAHESELADSPSDILELRFWASYRAQTLARTVRGMMYYRKALMLQTYLERLNSGVATSDLEAAISSSDATDTQGFELSPQARAQADLKFTYVVTCQIYGKQKEDKKPEAADIALLMQRNEALRVAFIDDVESLTEGKVHTEYYSKLVKGDINGKDKEIYSIKLPGNPKLGEGKPENQNHAIIFTRGNAIQTIDMNQDNYFEEALKMRNLLEEFHRDHGIRPPTILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIFAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGYYFCTMLTVLTVYIFLYGKTYLALSGVGETIQIRARILDNTALTTALNTQFLFQIGIFTAVPMVLGFILEQGFLRAVVSFVTMQLQLCSVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFVKGLEVVLLLVVYLAYGYNESGAIGYILLSISSWFMSLSWLFAPYLFNPSGFEWQKTVEDFRDWTNWLLYRGGIGVKGAESWEAWWDEELSHIRTLEGRIVETILSLRFFIFQYGVVYKLDVQGSDKSLTVYGLSWIVLAVLIILFKVFTFSQKISVNFQLVLRFVQGVSFLMALAGLAVAIILTDLTVSDIFACILAFVPTGWGILSIAVAWKPLLKKMGLWKSIRSIARLYDAGMGMLIFVPVALLSWFPFVSTFQTRLMYNQAFSRGLEISLILAGNNANSGI 1827          

BLAST of g.barbadense_cottongen_reftransV1_0094177 vs. ExPASy TrEMBL
Match: D7L640_ARALL (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_340908 PE=4 SV=1)

HSP 1 Score: 2881.66 bits (7469), Expect = 0.000e+0
Identity = 1452/1889 (76.87%), Postives = 1647/1889 (87.19%), Query Frame = -2

Query:  224 GSVGHAAGGIAGYVPSSL-NNRDIDTILRVADEIQDEEPNVARILCEHAYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGTIDRSQDVARLLEFYRLYREKNNVDKLREEEMMLRESGVFSGNLGELERKTLKRKRVFGTLRVLGMVLEQLTEEIPVELKRVIDSDAAMTEDLIAYNIIPFPLDAPTITNAIVSFPEVRAAVSALKHYSSLPKLPSDFSIPETRSPDLMDFLHYVFGFQKDNVSNQREHVVLLLANEQSRHGIPEEPEPKLDEAAVQKVFLKSLDNYIKWCNYLCIQPVWSNLDAVNKEKKILFVSLYFLIWGEAANIRFLPECLCYIFHHMAREMDEALRQHIAQPANSC-------SKDGVVSFLDQVITPLYDVVXXXXXXXXNGRAPHSAWRNYDDFNEYFWSLHCFDLSWPWR-KTSFFQKPEPRSKNPLKLGGGQHRGKTSFVEHRTFFHLYHSFHRLWIFLVMMFQGLTIIAFNDGHLNA-KTLREVLSLGPTFVVMKFTESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFSIASVFVSFLYVRALQEESKPNSNSVVFRXXXXXXXXXXXIHFFISFLMRIPACHRLTELCDNLSLIRFIKWMRQERYYVGRGMYERTTDFIKYMIFWLIILSGKFAFAYFFQIKPLVKPTRTVIAMDNIEYSWHDFVSRNNHNALTVVSLWAPVIAMYLLDIYIFYTVLSAVWGFLLGARDRLGEIRSLDAVQKLFEEFPDAFMKRLH-PV-RXXXXXXXXXXXXXKFDAARFSPFWNEIIKNLREEDYLTNFEMELLFMPKNTGKLPLVQWPLFLLASKIFLAKDIAAESRDSQDELWERISRDEYMKYAVQECYYALRYILTAILEAEGRTWVERIYEGIEASITKKTISDDFQLNKLQLVISRVTALLGILNQAEKPEHEKGAVNAVQDLYDVVRHDVLAIYLREHSDQWQSILKARTEGRLFAKLNWPRDPELKAQVKRLYSLLTIKDSASNVPKNLEARRRLEFFTNSLFMDMPPARPVQEMLSFSVFTPYYSEIVLYSMNELLKKNEDGISILFYLQKIYPDEWKNFLARIGRDENAAETELYDSPSDILELRFWASYRGQTLARTVRGMMYYRKALMLQTYLERENARDTEAALSRLETTDTQGYELSPEARARADLKFTYVVTCQIYGRQKEEQKPEAADIALLMQRNEALRVAFIDVVETLKDGKVHTEYYSKLVKADINGKDKEIYAIKLPGDPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKVRNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQESSFVTLGQRVLATPLKVRMHYGHPDVFDRVFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLFDFFRMMSFYFTTVGFYFCTMLTVLTIYIFLYGRAYLALSGVGETMQVRARITDNAALEAALNTQFLFQIGIFSAVPMVLGFILEQGFLRAIVSFITMQLQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVRHIKFSENYRLYSRSHFXXXXXXXXXXXXXLAYGYNDX-XXXXXXXXXXSWFMALSWLFAPYLFNPSGFEWQKIVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMAHIRTMRGRILETILSLRFFLFQYGVVYKLNVQGTNTSLTVYGFSWVVLAVLIILFKVFTFSQKMSVNFQLLLRFIQGVSFMXXXXXXXXXXALTDLSIPDIFASILAFVPTGWGILSIAAAWKPFVKKTGLWKSVRSMARLYDAGMGMIIFVPVAFFSWFPFVSTFQTRLMFNQAFSRGLEISLILAGNNPNTGL 5851
            G  G     I GYVPSSL NNRDID ILR ADEIQDE+PN+ARILCEH YSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVG IDRSQD+ RL EFYRLYREKNNVD L+EEE  LRESG F+    ELERKT+KRKRVF TL+VLG VLEQL +EIP ELK VIDSDAAM+ED IAYNIIP  LDAP  TNA  +FPEV+AAV+ALK++  LPKLP+DF IP TR  D++DFLHY+FGFQKD+VSNQREH+VLLLANEQSR  IPEE EPKLD+AAV KVFLKSL+NYIKWC+YLCIQP WSNL+A++ EKK+LF+SLYFLIWGEAANIRFLPECLCYIFHHM REMDE LRQ +A+PA SC       S DGV SFLD VI PLY VV+AEA NN+NGRAPHSAWRNYDDFNEYFWSLH F+L WPWR  +SFFQKP PR K  LK G  +HRGKTSFVEHRTF HLYHSFHRLWIFL MMFQ L IIAFN   L + KTLRE+LSLGPTFVVMKF+ESVLDVIMMYGAYSTTRRLAVSRIFLRFIWF +ASVF+SFLYV+AL+E   PNS+S +F+LYLIVI IYGG+ FF S LMRIP CH +   CD   +IRF KWMRQER+YVGRGMYERT+DFIKY++FWL++LS KF+FAYF QI+PLV PTR ++  +NI YSWHDFVSR N+NALTV SLWAPV+A+YLLDI+IFYT++SA  GFLLGARDRLGEIRSL+A+ KLFEEFP AFM+ LH P+   ++ +S + V+K+K DAA F+PFWN+IIK+LREEDY+T+FEMELL MPKN+G+L LVQWPLFLL+SKI LAK+IAAES +SQ+E+ ERI RD+YMKYAV+E Y+ L+ +LT  LEAEGR WVERI++ I+AS+ ++ I  DFQLNKL LVI+RVTA LGIL + E PEHEKGA+ A+QDLYDV+R D+L   +R H + W  + +A  EGRLF KL WP+DPE+KA VKRLYSL TIKDSA++VP+NLEARRRL+FFTNSLFMD+PP + V++MLSFSVFTPYYSE+VLYSM EL K+NEDGISILFYLQKIYPDEWKNFLARIGRDENA E +L D+  DI+ELRFWASYRGQTLARTVRGMMYYRKALMLQ+YLER+  RD E A      TD +G+ELSPEARA+ADLKFTYVVTCQIYGRQKE+QKPEA DIALLMQRNEALR+A+IDVV+T K+GK HTEYYSKLVKADI+GKDKEIY+IKLPGDPKLGEGKPENQNHAIVFTRGNA+QTIDMNQDNYFEEALK+RNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQE+SFVTLGQRVLA PLK+RMHYGHPDVFDRVFHITRGGISKASR+INISEDI+AGFN+TLRQGN+THHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQL DFFRMMSF+FTTVGFY CTMLTVLT+YIFLYGRAYLALSGVG T++ RA + D+ AL AALN QFLFQIG+F+AVPMVLGFILEQGFL+AIVSFITMQ QLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVV+HIKFSENYRLYSRSHFVKG+EV+LLLVVYLAYG +++ A+SYILL++SSWF+A+SWLFAPYLFNP+GFEWQK+VEDF++WTNWLFYRGGIGVKG ESWEAWW+EEM                       YG+VYKL +QG++TS  VYG+SWV  A+ I+LFKVFTFSQK+SVNFQLLLRFIQG+S ++A+AG+ VAV LT LS+ DIFA +LAF+PTGWGILSIA AWKP +K+ G+WKS+RS+ARLYDA MGM+IF+PVA  +WFPFVSTFQTR+MFNQAFSRGLEISLILAG+NPN+GL
Sbjct:   23 GVAGGNQSSIVGYVPSSLSNNRDIDAILRAADEIQDEDPNIARILCEHGYSLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREVGNIDRSQDILRLQEFYRLYREKNNVDTLKEEEKQLRESGAFTD---ELERKTVKRKRVFATLKVLGSVLEQLAKEIPEELKHVIDSDAAMSEDTIAYNIIP--LDAPVTTNATTTFPEVQAAVAALKYFPGLPKLPADFPIPVTRIADMLDFLHYIFGFQKDSVSNQREHIVLLLANEQSRLNIPEETEPKLDDAAVHKVFLKSLENYIKWCDYLCIQPAWSNLEAISGEKKLLFLSLYFLIWGEAANIRFLPECLCYIFHHMVREMDEILRQQVARPAESCMPVDSRGSDDGV-SFLDHVIAPLYGVVSAEAFNNDNGRAPHSAWRNYDDFNEYFWSLHSFELGWPWRTSSSFFQKPIPRKKYELKTGRAKHRGKTSFVEHRTFLHLYHSFHRLWIFLAMMFQALAIIAFNKDDLTSTKTLREILSLGPTFVVMKFSESVLDVIMMYGAYSTTRRLAVSRIFLRFIWFGLASVFISFLYVKALKE---PNSDSPIFKLYLIVIAIYGGVQFFFSILMRIPTCHNIANKCDRWPVIRFFKWMRQERHYVGRGMYERTSDFIKYLLFWLVVLSAKFSFAYFLQIEPLVSPTRMIVKQNNIPYSWHDFVSRKNYNALTVASLWAPVVAIYLLDIHIFYTIVSAFLGFLLGARDRLGEIRSLEAIHKLFEEFPGAFMRALHVPLTNRTSDTSHQAVDKNKVDAAHFAPFWNQIIKSLREEDYITDFEMELLLMPKNSGRLELVQWPLFLLSSKILLAKEIAAES-NSQEEILERIERDDYMKYAVEEVYHTLKLVLTETLEAEGRMWVERIFDDIKASLKERNIHHDFQLNKLSLVITRVTAFLGILKENETPEHEKGAIKALQDLYDVMRLDILTFNMRGHYETWNILTQAWNEGRLFTKLKWPKDPEMKALVKRLYSLFTIKDSAAHVPRNLEARRRLQFFTNSLFMDVPPPKSVRKMLSFSVFTPYYSEVVLYSMAELTKRNEDGISILFYLQKIYPDEWKNFLARIGRDENALEGDL-DNERDIIELRFWASYRGQTLARTVRGMMYYRKALMLQSYLERKAGRDDEDA------TDAEGFELSPEARAQADLKFTYVVTCQIYGRQKEDQKPEAVDIALLMQRNEALRIAYIDVVDTPKEGKSHTEYYSKLVKADISGKDKEIYSIKLPGDPKLGEGKPENQNHAIVFTRGNAIQTIDMNQDNYFEEALKMRNLLEEFDRDHGIRPPTILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLAKPLKIRMHYGHPDVFDRVFHITRGGISKASRVINISEDIFAGFNTTLRQGNVTHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLLDFFRMMSFFFTTVGFYLCTMLTVLTVYIFLYGRAYLALSGVGATIRERAILLDDTALSAALNAQFLFQIGVFTAVPMVLGFILEQGFLQAIVSFITMQFQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVKHIKFSENYRLYSRSHFVKGMEVILLLVVYLAYGNDEAGAVSYILLTVSSWFLAVSWLFAPYLFNPAGFEWQKVVEDFKEWTNWLFYRGGIGVKGAESWEAWWEEEM-----------------------YGIVYKLQLQGSDTSFAVYGWSWVAFAMSIVLFKVFTFSQKISVNFQLLLRFIQGLSLLMALAGIIVAVVLTKLSVTDIFACVLAFIPTGWGILSIACAWKPVLKRMGMWKSIRSLARLYDALMGMLIFLPVALCAWFPFVSTFQTRMMFNQAFSRGLEISLILAGDNPNSGL 1871