g.barbadense_cottongen_reftransV1_0094088, g.barbadense_cottongen_reftransV1_0094088 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy Swiss-Prot
Match: RF2_STRPN (Peptide chain release factor 2 OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) GN=prfB PE=3 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 1.055e-44
Identity = 77/155 (49.68%), Postives = 115/155 (74.19%), Query Frame = -2

Query:  259 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHS 723
             TSF+ VEVMP L +D+++VE+ E+D+++   R+GG GGQNVNKV T VR+THIPTG+ V+ T +R+Q  N+ +A+  L+AKL  + ++++A+E+  ++G+  +  WG QIR+YVF PY +VKD RT FE + +  VMDG+LD FI AYLK++ S
Sbjct:  211 HTSFTSVEVMPEL-DDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRTQYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDHRTSFEVAQVDKVMDGDLDGFIDAYLKWRIS 364          

HSP 2 Score: 147.902 bits (372), Expect = 3.924e-37
Identity = 82/216 (37.96%), Postives = 132/216 (61.11%), Query Frame = -2

Query: 1279 DFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 1926
            D   +R+ ++   E++   R S  ++ LE+E+ +LE+K  +  FW+D   AQ+T   L ++K+  N  ++ +   ++ E +++   E    D ++ +E V  + +L+K +  +E+T LLS PYD   A++ I  G+GGT+AQDW DMLLRMYTR+G  + +K  V++   G+EAGIKSVT+  EG  AYG L  E G HR+VR SPF++   R  +
Sbjct:    2 DISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPDFWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAE----DESVHDELVAQLAELDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTS 213          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy Swiss-Prot
Match: RF2_STRU0 (Peptide chain release factor 2 OS=Streptococcus uberis (strain ATCC BAA-854 / 0140J) GN=prfB PE=3 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 2.135e-44
Identity = 76/153 (49.67%), Postives = 112/153 (73.20%), Query Frame = -2

Query:  265 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYK 723
             TSF+ VEVMP L +D+++V++ ++D+++   R+GG GGQNVNKV T VR+THIPTG+ V  T +R+Q  N+ +A+  L+AKL  + +E++A E+  ++GD  +  WG QIR+YVF PY +VKD RT FE + +  VMDGE+D FI AYLK++
Sbjct:  211 HTSFTSVEVMPEL-DDTIEVDIRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVASTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAEEVNALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFEVAQVDKVMDGEIDGFIDAYLKWR 362          

HSP 2 Score: 135.961 bits (341), Expect = 4.724e-33
Identity = 81/212 (38.21%), Postives = 122/212 (57.55%), Query Frame = -2

Query: 1279 LRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 1914
            +R+ +    E++   R S  +  LE+E+ +LE+K  +  FW+D   AQ+T   L  +K      ++     ++ E  + +  E +SI   L E  V     L + L ++E+T LLS PYD   A++ I  G+GGT+AQDWADML RMYTR+G  + +K   ++   G+EAGIKSVT+  EG  AYG+L  E G HR+VR SPF++   R  +
Sbjct:    6 IRQKIVENKEKLTSFRRSLDLDGLEEEIALLENKMTEPDFWNDNIAAQKTSQELNTLKSTFETFHDMLELSDETELYLEMLAEDESIKEELEEALVK----LEQILSQYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWADMLFRMYTRFGNAKGFKVETLDYQAGDEAGIKSVTLSFEGPNAYGFLKSEMGVHRLVRISPFDSAKRRHTS 213          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy Swiss-Prot
Match: RF2_CLOP1 (Peptide chain release factor 2 OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) GN=prfB PE=3 SV=2)

HSP 1 Score: 167.548 bits (423), Expect = 3.876e-44
Identity = 80/150 (53.33%), Postives = 104/150 (69.33%), Query Frame = -2

Query:  274 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYL 723
            QTSF+ VEV+P L  D  D+E+   DL I   RAGG GGQ+VNK E+AVRITHIPTG+ V+C  ERSQ +N+  A+  LK+KL+ + E     +I+ + G+     WG QIR+YVFHPY +VKD RT  ETS++  VMDG++D FI AYL
Sbjct:  212 QTSFASVEVLPELTSDQ-DIEINPVDLRIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQNERSQFSNRDTAMGMLKSKLIELKERAHKEKIEDLTGELKDMGWGSQIRSYVFHPYSMVKDHRTNVETSNVNGVMDGDIDNFIIAYL 360          

HSP 2 Score: 159.844 bits (403), Expect = 2.805e-41
Identity = 88/201 (43.78%), Postives = 126/201 (62.69%), Query Frame = -2

Query: 1279 VNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 1881
            + E+  S  +  LEK+   LE K  ++ FWDD  KAQE       VKDKI+       +++D E +  L EE D   A   +E ++ +K L+K +D  ++  +LSG YD+  A++++  G GG+DA DW +MLLRMYTRW EK+ Y    ++   G+EAG+KSVT++V+G +AYGYL  EKG HR+VR SPFNA G RQ +
Sbjct:   17 LKEMGASLCLASLEKKSAELELKMQEAGFWDDVQKAQEVTQEAKRVKDKIDKFKNLNERIDDVEVLKELMEENDEETA---KEIISEVKALSKEIDTLKIETILSGEYDRNDAILTLHTGVGGSDANDWTEMLLRMYTRWCEKKGYSLETIDYLPGDEAGVKSVTLKVKGEFAYGYLKAEKGIHRLVRISPFNANGKRQTS 214          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy Swiss-Prot
Match: RF2_STRA1 (Peptide chain release factor 2 OS=Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700) GN=prfB PE=3 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 6.385e-44
Identity = 76/153 (49.67%), Postives = 112/153 (73.20%), Query Frame = -2

Query:  265 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYK 723
             TSF+ VEVMP L +D+++VE+ ++D+++   R+GG GGQNVNKV T VR+THIPTG+ V  T +R+Q  N+ +A+  L+AKL  + +E++A E+  ++GD  +  WG QIR+YVF PY +VKD RT FE + +  VMDGE++ FI AYLK++
Sbjct:  211 HTSFASVEVMPEL-DDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFELAQVDKVMDGEINGFIDAYLKWR 362          

HSP 2 Score: 140.198 bits (352), Expect = 1.834e-34
Identity = 81/212 (38.21%), Postives = 130/212 (61.32%), Query Frame = -2

Query: 1279 LRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 1914
            +R+ +    E++   R S  + +LE+E+ +LE++  +  FW+D   AQ+T   L ++K K +  +  +   ++ E ++ + +E DS+   L E   N+++ L+K +  +E+T LLS PYD   A++ I  G+GGT+AQDW D+LLRMYTR+G    +K  V++   G+EAGIKSVT+  EG  AYG L  E G HR+VR SPF++   R  +
Sbjct:    6 IRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPDFWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEE---NLMQ-LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTS 213          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy Swiss-Prot
Match: RF2_THEP1 (Peptide chain release factor 2 OS=Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995) GN=prfB PE=3 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.720e-43
Identity = 82/152 (53.95%), Postives = 108/152 (71.05%), Query Frame = -2

Query:  268 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKY 723
             TSF+ V V+P + +D VD+E+  EDL+I   RA G GGQ VNK E+AVRITH+PTG+ V C  ERSQ  NK  AL  LKAKL  +  E++  EI++I+G+     WG QIR+YVFHPY +VKD RTG ET+++ +VMDG++D FI+A L Y
Sbjct:  211 HTSFASVNVIPEI-DDDVDIEIRPEDLKIETFRASGHGGQYVNKTESAVRITHLPTGIVVSCQNERSQHQNKQTALKILKAKLYQLEMEKKQREIQEIQGELKDISWGNQIRSYVFHPYTMVKDHRTGVETANVDAVMDGDIDMFIEAELVY 361          

HSP 2 Score: 137.502 bits (345), Expect = 1.766e-33
Identity = 80/214 (37.38%), Postives = 124/214 (57.94%), Query Frame = -2

Query: 1279 YALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 1920
            +  R  +E   ++  +I +     ++++E+  +E K  D S WDD+ KA+E    L  +K+    L   ++  ED E  + L++E    D  + +    ++++L  A+ + EL  +L+G YD   A +S+  GAGGT++QDWA MLLRMY RW E++ +   +VE   GEEAGIK  TI ++G YAYGYL  E G HR+VR SPF+A   R  +
Sbjct:    4 FETRTRMEELEKKYKDILSVVNENEVDREIEEIEKKLTDPSVWDDQKKAREYTQKLKRLKNISEDLKRVRSLFEDLEVAIELSDE----DQEMAQHVEEIVQELEGAVKKLELEIILNGKYDPNNAYLSVHPGAGGTESQDWAQMLLRMYMRWAERKGFDVEIVEFQPGEEAGIKDATILIKGEYAYGYLKHESGVHRLVRISPFDAARRRHTS 213          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy Swiss-Prot
Match: RF2_THESQ (Peptide chain release factor 2 OS=Thermotoga sp. (strain RQ2) GN=prfB PE=3 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 2.736e-43
Identity = 81/152 (53.29%), Postives = 108/152 (71.05%), Query Frame = -2

Query:  268 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKY 723
             TSF+ V V+P + +D VD+E+  EDL+I   RA G GGQ VNK E+AVRITH+PTG+ V C  ERSQ  NK  AL  LKAKL  +  E++  EI++I+G+     WG QIR+Y+FHPY +VKD RTG ET+++ +VMDG++D FI+A L Y
Sbjct:  211 HTSFASVNVIPEI-DDDVDIEIRPEDLKIETFRASGHGGQYVNKTESAVRITHLPTGIVVSCQNERSQHQNKQTALKILKAKLYQLEMEKKRREIQEIQGELKDISWGNQIRSYIFHPYTMVKDHRTGVETANVDAVMDGDIDMFIEAELVY 361          

HSP 2 Score: 137.117 bits (344), Expect = 1.887e-33
Identity = 80/214 (37.38%), Postives = 123/214 (57.48%), Query Frame = -2

Query: 1279 YALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 1920
            +  +  +E   ++  ++ +     ++ KEL  +E K  D S WDD+ KA+E    L  +K+    L   ++  ED E  + L++E    D  + +    ++++L  A+ + EL  +L+G YD   A +S+  GAGGT++QDWA MLLRMY RW E++ +   +VE   GEEAGIK  TI ++G YAYGYL  E G HR+VR SPF+A   R  +
Sbjct:    4 FETKTKIEELEKKYKDVLSVVNEDEINKELEEVEKKLTDPSVWDDQKKAREYTQKLKRLKNISEDLKRVRSLFEDLEVAIELSDE----DQEMAQHVEEIVQELEGAVKKLELEIILNGKYDPNNAYLSVHPGAGGTESQDWAQMLLRMYMRWAERKGFDVEIVEFQPGEEAGIKDATILIKGEYAYGYLKHESGVHRLVRISPFDAARRRHTS 213          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy Swiss-Prot
Match: RF2_STRPG (Peptide chain release factor 2 OS=Streptococcus pyogenes serotype M5 (strain Manfredo) GN=prfB PE=3 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 2.757e-43
Identity = 75/153 (49.02%), Postives = 112/153 (73.20%), Query Frame = -2

Query:  265 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYK 723
             TSF+ VEVMP L +++++VE+ ++D+++   R+GG GGQNVNKV T VR+THIPTG+ V  T +R+Q  N+ +A+  L+AKL  + +E++A E+  ++GD  +  WG QIR+YVF PY +VKD RT FE + +  VMDGE++ FI AYLK++
Sbjct:  211 HTSFASVEVMPEL-DNTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFELAQVDKVMDGEINGFIDAYLKWR 362          

HSP 2 Score: 139.813 bits (351), Expect = 2.556e-34
Identity = 81/212 (38.21%), Postives = 129/212 (60.85%), Query Frame = -2

Query: 1279 LRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 1914
            +R+ +    E++   R S  + +LE+E+ +LE+   +  FW+D   AQ+T   L ++K K +  +  +   ++ E ++ + +E DS+   L E   N+++ L+K +  +E+T LLS PYD   A++ I  G+GGT+AQDW D+LLRMYTR+G    +K  V++   G+EAGIKSVT+  EG  AYG L  E G HR+VR SPF++   R  +
Sbjct:    6 IRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPDFWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEE---NLMQ-LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTS 213          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy Swiss-Prot
Match: RF2_THEMA (Peptide chain release factor 2 OS=Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) GN=prfB PE=3 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 3.056e-43
Identity = 81/152 (53.29%), Postives = 108/152 (71.05%), Query Frame = -2

Query:  268 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKY 723
             TSF+ V V+P + +D VD+E+  EDL+I   RA G GGQ VNK E+AVRITH+PTG+ V C  ERSQ  NK  AL  LKAKL  +  E++  EI++I+G+     WG QIR+Y+FHPY +VKD RTG ET+++ +VMDG++D FI+A L Y
Sbjct:  213 HTSFASVNVIPEI-DDDVDIEIRPEDLKIETFRASGHGGQYVNKTESAVRITHLPTGIVVSCQNERSQHQNKQTALKILKAKLYQLEMEKKRREIQEIQGELKDISWGNQIRSYIFHPYTMVKDHRTGVETANVDAVMDGDIDMFIEAELVY 363          

HSP 2 Score: 137.502 bits (345), Expect = 1.805e-33
Identity = 80/214 (37.38%), Postives = 123/214 (57.48%), Query Frame = -2

Query: 1279 YALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 1920
            +  +  +E   ++  ++ +     ++ KEL  +E K  D S WDD+ KA+E    L  +K+    L   ++  ED E  + L++E    D  + +    ++++L  A+ + EL  +L+G YD   A +S+  GAGGT++QDWA MLLRMY RW E++ +   +VE   GEEAGIK  TI ++G YAYGYL  E G HR+VR SPF+A   R  +
Sbjct:    6 FETKTKIEELEKKYKDVLSVVNEDEINKELEEVEKKLTDPSVWDDQKKAREYTQKLKRLKNISEDLKRVRSLFEDLEVAIELSDE----DQEMAQHVEEIVQELEGAVKKLELEIILNGKYDPNNAYLSVHPGAGGTESQDWAQMLLRMYMRWAERKGFDVEIVEFQPGEEAGIKDATILIKGEYAYGYLKHESGVHRLVRISPFDAARRRHTS 215          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy Swiss-Prot
Match: RF2_CLOPS (Peptide chain release factor 2 OS=Clostridium perfringens (strain SM101 / Type A) GN=prfB PE=3 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 3.355e-43
Identity = 79/150 (52.67%), Postives = 103/150 (68.67%), Query Frame = -2

Query:  274 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYL 723
            QTSF+ VEV+P L  D  D+E+   DL I   RAGG GGQ+VNK E+AVRITHIPTG+ V+C  ERSQ +N+  A+  LK+KL+ + E     +I+ + G+     WG QIR+YVFHPY +VKD RT  ETS++  VM G++D FI AYL
Sbjct:  214 QTSFASVEVLPELTSDQ-DIEINPVDLRIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQNERSQFSNRDTAMGMLKSKLIELKERAHKEKIEDLTGELKDMGWGSQIRSYVFHPYSMVKDHRTNVETSNVNGVMGGDIDNFIIAYL 362          

HSP 2 Score: 159.458 bits (402), Expect = 3.067e-41
Identity = 88/201 (43.78%), Postives = 126/201 (62.69%), Query Frame = -2

Query: 1279 VNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 1881
            + E+  S  +  LEK+   LE K  ++ FWDD  KAQE       VKDKI+       +++D E +  L EE D   A   +E ++ +K L+K +D  ++  +LSG YD+  A++++  G GG+DA DW +MLLRMYTRW EK+ Y    ++   G+EAG+KSVT++V+G +AYGYL  EKG HR+VR SPFNA G RQ +
Sbjct:   19 LKEMGASLDLASLEKKSAELELKMQEAGFWDDVQKAQEVTQEAKRVKDKIDKFKNLNERIDDVEVLKELMEENDEETA---KEIISEVKALSKEIDTLKIETILSGEYDRNNAILTLHTGVGGSDANDWTEMLLRMYTRWCEKKGYSLETIDYLPGDEAGVKSVTLKVKGEFAYGYLKAEKGIHRLVRISPFNANGKRQTS 216          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy Swiss-Prot
Match: RF2_OENOB (Peptide chain release factor 2 OS=Oenococcus oeni (strain ATCC BAA-331 / PSU-1) GN=prfB PE=3 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 3.640e-42
Identity = 78/155 (50.32%), Postives = 107/155 (69.03%), Query Frame = -2

Query:  259 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHS 723
             TSF  ++VMP L +D +++E+  +D+++   R+GG GGQNVNKV TAVR+THIPTG+ V    ER+Q  N+  A+  LKAKL    E++R  E  ++ G  +   WG QIR+YVF PY++VKD+R+G+ET D   VMDG LD FI AYLK+K S
Sbjct:  213 HTSFVSIDVMPELDDDEIEIEIKPQDVKMDVYRSGGAGGQNVNKVSTAVRLTHIPTGIVVASQVERTQYGNRDIAMKMLKAKLYEQEEQKREEEHAKLSGTKLDVAWGSQIRSYVFQPYRMVKDLRSGYETGDTDGVMDGNLDPFINAYLKWKLS 367          

HSP 2 Score: 121.709 bits (304), Expect = 3.636e-28
Identity = 73/213 (34.27%), Postives = 112/213 (52.58%), Query Frame = -2

Query: 1273 RKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVTSV 1911
            R  +    E+++  R++  +  L+  +   E K +   FWDD+  AQ+ +     +K++ +     +  VED   +  L  E D  DA +  E    I+   K L+++ L QLL+  YD   A++ I  G GGT++ DWA  L RMYTRW +   +K  V +   G+ AGI S T+ + G  AYG+L  EKG HR VR SPF++ G R  + V
Sbjct:    7 RNSISDMQEQIDGFRSTLDLDALDASIAENEDKMSQPGFWDDQQSAQKIIDETNTLKNRRDSFLSLQKSVEDLTAMAELLSEED--DADMHAELDTDIEKTEKDLEKYNLNQLLTEKYDSNNAILEIHPGEGGTESTDWASNLYRMYTRWAQAHDFKVEVTDYQTGDVAGIDSATLRIIGHNAYGFLRSEKGVHRFVRISPFDSAGRRHTSFV 217          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy TrEMBL
Match: A0A0D2R4L4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G264100 PE=4 SV=1)

HSP 1 Score: 530.406 bits (1365), Expect = 1.380e-177
Identity = 292/299 (97.66%), Postives = 295/299 (98.66%), Query Frame = -2

Query: 1279 MEAILNPLLSSPTILKPTNPFSLSNVNKAXXXXXXXXXXXXXXXXSAILSSPPPRFTSSVTYATSDSQLSEGVETSTREWVMQDFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 2175
            M+AILNPLLSSPTILKPTNPFSLSNVNKAPFLLSLTHFTTTSFPFSAILS+PPPRFTSSVTYATSDSQLSEGVETSTREWVMQDFYALRKDVE ASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVI DLNKALDRFELTQLLSGPYDKEGAVISI AGAGGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQ +
Sbjct:    1 MDAILNPLLSSPTILKPTNPFSLSNVNKAPFLLSLTHFTTTSFPFSAILSTPPPRFTSSVTYATSDSQLSEGVETSTREWVMQDFYALRKDVETASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVINDLNKALDRFELTQLLSGPYDKEGAVISITAGAGGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQTS 299          

HSP 2 Score: 325.094 bits (832), Expect = 2.309e-98
Identity = 156/157 (99.36%), Postives = 157/157 (100.00%), Query Frame = -2

Query:  253 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSIN 723
            QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKAL+RLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSIN
Sbjct:  297 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALTRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSIN 453          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy TrEMBL
Match: A0A0B0N7A0_GOSAR (Peptide chain release factor 2 OS=Gossypium arboreum GN=F383_11471 PE=4 SV=1)

HSP 1 Score: 527.324 bits (1357), Expect = 2.360e-176
Identity = 292/299 (97.66%), Postives = 294/299 (98.33%), Query Frame = -2

Query: 1279 MEAILNPLLSSPTILKPTNPFSLSNVNKAXXXXXXXXXXXXXXXXSAILSSPPPRFTSSVTYATSDSQLSEGVETSTREWVMQDFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 2175
            MEAILNPLLSS TILKP NPF LSNVNKAPFLLSLTHFTTTSFPFSAILSSPPPRFTSSVTYATSDSQLSEGVETSTREWVMQDFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEGAVISIAAGAGGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKS TIEVEGR+AYGYLSGEKGTHRIVRQSPFNAKGLRQ +
Sbjct:    1 MEAILNPLLSSSTILKPANPFYLSNVNKAPFLLSLTHFTTTSFPFSAILSSPPPRFTSSVTYATSDSQLSEGVETSTREWVMQDFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEGAVISIAAGAGGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSATIEVEGRFAYGYLSGEKGTHRIVRQSPFNAKGLRQTS 299          

HSP 2 Score: 327.791 bits (839), Expect = 2.442e-99
Identity = 158/159 (99.37%), Postives = 159/159 (100.00%), Query Frame = -2

Query:  247 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSINVS 723
            QTSFSGVEVMPLLPE+SVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSINVS
Sbjct:  297 QTSFSGVEVMPLLPEESVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSINVS 455          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy TrEMBL
Match: A0A061F5D6_THECC (High chlorophyll fluorescent 109 isoform 2 OS=Theobroma cacao GN=TCM_025105 PE=4 SV=1)

HSP 1 Score: 448.358 bits (1152), Expect = 1.107e-145
Identity = 244/302 (80.79%), Postives = 261/302 (86.42%), Query Frame = -2

Query: 1279 MEAILNPLLSSPTILKPTNPFSLSN---VNKAXXXXXXXXXXXXXXXXSAILSSPPPRFTSSVTYATSDSQLSEGVETSTREWVMQDFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 2175
            MEAIL PLLSS T++KP  P S S+   +NK P LLSL    T +      + S P RFTSSV YAT +SQL+EGV+T+TREWVMQDFYALRKDVE ASERV EI  S G+Q LEKEL  LESK ADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIV LTEEMD+IDAALLEEAV+VIK+LNKALDRFELTQLLSGPYDKEGAVISI AGAGGTDAQDWADMLLRMY RWGEKQKYKT+VVEKSLGEEAG+KS TIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQ +
Sbjct:    1 MEAILKPLLSSTTMIKPPKPSSFSSYTSINKVPVLLSLAPLLTATSFSFTAILSAPSRFTSSVIYATPESQLTEGVDTNTREWVMQDFYALRKDVETASERVEEIIASTGLQLLEKELADLESKAADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVKLTEEMDAIDAALLEEAVSVIKELNKALDRFELTQLLSGPYDKEGAVISITAGAGGTDAQDWADMLLRMYVRWGEKQKYKTKVVEKSLGEEAGLKSATIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQTS 302          

HSP 2 Score: 319.701 bits (818), Expect = 2.306e-96
Identity = 151/159 (94.97%), Postives = 158/159 (99.37%), Query Frame = -2

Query:  247 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSINVS 723
            QTSF+GVEVMPLLPE+SVDVELPEEDLEI+FSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDI SVMDGELDAFIKAYLK+K+S++VS
Sbjct:  300 QTSFAGVEVMPLLPEESVDVELPEEDLEITFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIASVMDGELDAFIKAYLKHKYSMSVS 458          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy TrEMBL
Match: A0A061EYJ6_THECC (High chlorophyll fluorescent 109 isoform 1 OS=Theobroma cacao GN=TCM_025105 PE=4 SV=1)

HSP 1 Score: 431.024 bits (1107), Expect = 6.369e-138
Identity = 244/334 (73.05%), Postives = 261/334 (78.14%), Query Frame = -2

Query: 1279 MEAILNPLLSSPTILKPTNPFSLSN---VNKAXXXXXXXXXXXXXXXXSAILSSPPPRFTSSVTYATSDSQLSEGVETSTREWVMQ--------------------------------DFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 2175
            MEAIL PLLSS T++KP  P S S+   +NK P LLSL    T +      + S P RFTSSV YAT +SQL+EGV+T+TREWVMQ                                DFYALRKDVE ASERV EI  S G+Q LEKEL  LESK ADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIV LTEEMD+IDAALLEEAV+VIK+LNKALDRFELTQLLSGPYDKEGAVISI AGAGGTDAQDWADMLLRMY RWGEKQKYKT+VVEKSLGEEAG+KS TIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQ +
Sbjct:    1 MEAILKPLLSSTTMIKPPKPSSFSSYTSINKVPVLLSLAPLLTATSFSFTAILSAPSRFTSSVIYATPESQLTEGVDTNTREWVMQGSEAVGMLPWFYGHYHCFEVVGFNANKVLDFVDFYALRKDVETASERVEEIIASTGLQLLEKELADLESKAADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVKLTEEMDAIDAALLEEAVSVIKELNKALDRFELTQLLSGPYDKEGAVISITAGAGGTDAQDWADMLLRMYVRWGEKQKYKTKVVEKSLGEEAGLKSATIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQTS 334          

HSP 2 Score: 310.842 bits (795), Expect = 3.768e-92
Identity = 149/155 (96.13%), Postives = 154/155 (99.35%), Query Frame = -2

Query:  259 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHS 723
            QTSF+GVEVMPLLPE+SVDVELPEEDLEI+FSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDI SVMDGELDAFIKAYLK+K+S
Sbjct:  332 QTSFAGVEVMPLLPEESVDVELPEEDLEITFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIASVMDGELDAFIKAYLKHKYS 486          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy TrEMBL
Match: B9RM42_RICCO (Peptide chain release factor, putative OS=Ricinus communis GN=RCOM_1077620 PE=4 SV=1)

HSP 1 Score: 392.504 bits (1007), Expect = 2.876e-124
Identity = 201/243 (82.72%), Postives = 218/243 (89.71%), Query Frame = -2

Query: 1279 TSSVTYATSDSQLSEGVETSTREWVMQDFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 2007
            +SSV YA  DSQL+ GV+T TREW MQDFY+LR+DVE ASERV EIRTSAG+QQLE+E+  LESK A++SFWDDRA AQETLLALTDVKDKI LL EFKTKVEDAETIV LTEEMDSID  LLEEA ++IK+LNK+L+RFELTQLLSGPYDKE AVISI AGAGGTDAQDWADMLLRMY RWGEKQ+YKTRVVEKS GEEAGIKS  IEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQ +
Sbjct:   58 SSSVIYAAPDSQLNVGVDTETREWAMQDFYSLRRDVEAASERVEEIRTSAGLQQLEQEVTNLESKAANTSFWDDRATAQETLLALTDVKDKIKLLTEFKTKVEDAETIVKLTEEMDSIDTGLLEEASSIIKELNKSLERFELTQLLSGPYDKESAVISITAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSPGEEAGIKSAMIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQTS 300          

HSP 2 Score: 312.768 bits (800), Expect = 9.498e-94
Identity = 147/159 (92.45%), Postives = 156/159 (98.11%), Query Frame = -2

Query:  247 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSINVS 723
            QTSFSG+EVMPLLPE+S+DVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIA EQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG+ETSD+ SVMDGEL+AFIKAYLKYK+S+ +S
Sbjct:  298 QTSFSGIEVMPLLPEESMDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAVEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDVTSVMDGELEAFIKAYLKYKYSMTLS 456          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy TrEMBL
Match: W9RF34_9ROSA (Peptide chain release factor 2 OS=Morus notabilis GN=L484_005584 PE=4 SV=1)

HSP 1 Score: 388.267 bits (996), Expect = 1.345e-122
Identity = 202/248 (81.45%), Postives = 215/248 (86.69%), Query Frame = -2

Query: 1279 PPPRFTSSVTYATSDSQLSEGVETSTREWVMQDFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 2022
            P  RF   V YAT + Q+   V    REW MQDFY+LRKDVE  SERV EIR SAG+QQLE+EL VLESK ADSSFWDDRAKAQETLLALTDVKDKI LL+EFK KVEDAETI+ LTEEMDSID ALLEEA  +IK+LNKALDRFELT+LLSGPYDKEGAVISI AGAGGTDAQDWADMLLRMY RWGEKQ+YKTRVVEKS GEEAGIKS TIEVEGRYAYGYLSGEKGTHRIVRQSPFN+KGLRQ +
Sbjct:   53 PLSRFPPYVLYATQERQVGTEVNNDVREWAMQDFYSLRKDVETVSERVEEIRASAGLQQLEEELAVLESKAADSSFWDDRAKAQETLLALTDVKDKIKLLSEFKAKVEDAETIIMLTEEMDSIDTALLEEANTIIKELNKALDRFELTELLSGPYDKEGAVISITAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSPGEEAGIKSATIEVEGRYAYGYLSGEKGTHRIVRQSPFNSKGLRQTS 300          

HSP 2 Score: 306.605 bits (784), Expect = 1.998e-91
Identity = 144/159 (90.57%), Postives = 156/159 (98.11%), Query Frame = -2

Query:  247 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSINVS 723
            QTSFSGVEVMPLLPE+S+++E+P+EDL+ISFSRAGGKGGQNVNKVETAVRITHIPTGVT+RCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDIVSVMDGEL+ FIKA+LKYK+S+ VS
Sbjct:  298 QTSFSGVEVMPLLPEESINIEIPDEDLDISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGLETSDIVSVMDGELEPFIKAFLKYKYSMAVS 456          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy TrEMBL
Match: D7SPM1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0023g00870 PE=4 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 2.105e-121
Identity = 200/248 (80.65%), Postives = 215/248 (86.69%), Query Frame = -2

Query: 1279 PPRFTSSVTYATSDSQLSEGVETSTR-EWVMQDFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 2019
            P RF+  V +A+ +SQL   V T T  EW MQDFY LR+DVE  SER  EIR SAG+QQLE+EL  LE K ADSSFWDDRAKAQETLLALTDVKDKI LL +FKT+VEDAETIV LTEEMDSID  LLEEA N+IK+LNKALDRFELTQLLSGPYDKEGAVISI AGAGGTDAQDWADMLLRMY RWGEKQ+YKTRVVEKS+GEEAGIKS TIE+EGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQ +
Sbjct:   45 PSRFSPHVLFASPESQLDMEVTTETSSEWAMQDFYTLRRDVEAISERAEEIRASAGLQQLEEELAALEMKAADSSFWDDRAKAQETLLALTDVKDKIKLLTDFKTQVEDAETIVKLTEEMDSIDTGLLEEAANIIKELNKALDRFELTQLLSGPYDKEGAVISITAGAGGTDAQDWADMLLRMYVRWGEKQRYKTRVVEKSMGEEAGIKSATIELEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQTS 292          

HSP 2 Score: 307.76 bits (787), Expect = 4.884e-92
Identity = 147/159 (92.45%), Postives = 155/159 (97.48%), Query Frame = -2

Query:  247 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSINVS 723
            QTSFSGVEVMPLLPE+S+DVE+PEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDI SVMDGEL+ FIKAYLK+K S+ +S
Sbjct:  290 QTSFSGVEVMPLLPEESMDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDITSVMDGELEPFIKAYLKFKFSMTLS 448          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy TrEMBL
Match: A0A067JVR4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18056 PE=4 SV=1)

HSP 1 Score: 379.793 bits (974), Expect = 2.128e-119
Identity = 211/302 (69.87%), Postives = 236/302 (78.15%), Query Frame = -2

Query: 1279 MEAILNPLLSSPTILKPTNPFSLSNVNKAXXXXXXXXXXXXXXXXSAILSS---PPPRFTSSVTYATSDSQLSEGVETSTREWVMQDFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 2175
            ME ++ PL SS T+L       LS+  K+  LLS+    ++        SS      R  SS+ YA  ++QL  GV+T T EW MQDFY+LR+DVE ASERV EI  SAGMQ L++EL  LE+K ADSS WDDRA+AQE L ALTDVKDKI LL EFKT VEDAETIV LTEEMDS DA LLEEA  ++K+LNK+LDRFELTQLLSGPYDKEGAVISI AGAGGTDAQDWADMLLRMY RWGEKQ+YKTRVVEKS GEEAGIKSVTIE+EGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQ +
Sbjct:    1 MEGLVCPLYSSNTVLASAK---LSHNIKSSVLLSVNPSVSSPHRTILAFSSVLATSSRLPSSIIYAAPENQLDVGVDTETGEWAMQDFYSLRRDVEAASERVEEIIASAGMQLLQQELAGLEAKAADSSLWDDRARAQEILSALTDVKDKIKLLTEFKTMVEDAETIVKLTEEMDSTDAGLLEEATAIVKELNKSLDRFELTQLLSGPYDKEGAVISITAGAGGTDAQDWADMLLRMYLRWGEKQRYKTRVVEKSPGEEAGIKSVTIEIEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQTS 299          

HSP 2 Score: 309.686 bits (792), Expect = 1.406e-92
Identity = 147/159 (92.45%), Postives = 155/159 (97.48%), Query Frame = -2

Query:  247 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSINVS 723
            QTSFSGVEVMPLLPE+S+DVE+PEEDLEI+FSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIA EQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDI SVMDGEL+ FIKAYLKYK+S+ +S
Sbjct:  297 QTSFSGVEVMPLLPEESMDVEIPEEDLEITFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAVEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDINSVMDGELEPFIKAYLKYKYSMAMS 455          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy TrEMBL
Match: B9HHX5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0008s07570g PE=4 SV=2)

HSP 1 Score: 375.17 bits (962), Expect = 1.657e-117
Identity = 199/257 (77.43%), Postives = 217/257 (84.44%), Query Frame = -2

Query: 1279 LSSPPPRFTSSVTYATSDSQLSEGVETST------REWVMQDFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 2031
            L S P R  SSV YAT DSQLS G +T T      REW MQDFY+LRKDVE AS+RV E+R S+G+  LE+EL  LESK ADSS WDDRAKAQETLLALTDVKDKI LL EFKTKVE+AETIV LTEEMDS DA  LEEA  +IK+LNKALD++ELTQLLSGPYDKEGAV+SI AGAGGTDAQDWADMLLRMY RWGEKQ+YKT+VVE S GEEAGIKS T+EVEGRYAYGYLSGEKGTHRIVRQSPFN+KGLRQ +
Sbjct:   49 LFSAPSRSFSSVIYATPDSQLSVGADTDTDTDTDTREWAMQDFYSLRKDVETASQRVEEMRASSGLHLLEQELADLESKAADSSLWDDRAKAQETLLALTDVKDKIKLLAEFKTKVEEAETIVKLTEEMDSTDAGFLEEATGIIKELNKALDKYELTQLLSGPYDKEGAVLSITAGAGGTDAQDWADMLLRMYVRWGEKQRYKTKVVESSPGEEAGIKSATVEVEGRYAYGYLSGEKGTHRIVRQSPFNSKGLRQTS 305          

HSP 2 Score: 306.99 bits (785), Expect = 1.350e-91
Identity = 146/159 (91.82%), Postives = 154/159 (96.86%), Query Frame = -2

Query:  247 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSINVS 723
            QTSFSGVEVMPLLPE+S+DVELPEEDLEIS SRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQL+NKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTG ETSDI SVMDGELD FI+AYLK+K+S+ +S
Sbjct:  303 QTSFSGVEVMPLLPEESMDVELPEEDLEISTSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLSNKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETSDIASVMDGELDPFIRAYLKHKYSMTLS 461          

BLAST of g.barbadense_cottongen_reftransV1_0094088 vs. ExPASy TrEMBL
Match: A0A059D2I3_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B01462 PE=4 SV=1)

HSP 1 Score: 370.933 bits (951), Expect = 4.854e-116
Identity = 191/252 (75.79%), Postives = 217/252 (86.11%), Query Frame = -2

Query: 1279 ILSSPPPRFTSSVTYATSDSQLSEGVETSTREWVMQDFYALRKDVEMASERVNEIRTSAGMQQLEKELVVLESKTADSSFWDDRAKAQETLLALTDVKDKINLLNEFKTKVEDAETIVNLTEEMDSIDAALLEEAVNVIKDLNKALDRFELTQLLSGPYDKEXXXXXXXXXXXGTDAQDWADMLLRMYTRWGEKQKYKTRVVEKSLGEEAGIKSVTIEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQVT 2034
            + SS   R   SV  + S+ QLS+ VE  + EW MQDFY+LR++VE AS RV +IR SAG+QQLEKEL  LE K +D+SFWDDRAKAQ+TLLALTDVKDKI LL++FKT+VE+AET+V LTEEMDS D  LLEEA  +IK+LNKALDRFELT+LLSGPYDKEGAV+SI AGAGGTDAQDWADMLLRMY RWGEKQ+YKTRVVEKS+GEEAGIKS T+EVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQ +
Sbjct:   38 VPSSSRFRSVGSVVCSASNDQLSQSVEADSSEWAMQDFYSLRREVEAASARVVDIRASAGLQQLEKELADLELKASDASFWDDRAKAQQTLLALTDVKDKIRLLSDFKTQVEEAETVVMLTEEMDSKDTGLLEEAAGIIKELNKALDRFELTELLSGPYDKEGAVVSITAGAGGTDAQDWADMLLRMYARWGEKQRYKTRVVEKSMGEEAGIKSATLEVEGRYAYGYLSGEKGTHRIVRQSPFNAKGLRQTS 289          

HSP 2 Score: 305.449 bits (781), Expect = 4.162e-91
Identity = 144/159 (90.57%), Postives = 154/159 (96.86%), Query Frame = -2

Query:  247 QTSFSGVEVMPLLPEDSVDVELPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTVRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGFETSDIVSVMDGELDAFIKAYLKYKHSINVS 723
            QTSFSGVEVMPLLPE+S++VE+PEEDLEI+FSRAGGKGGQNVNKVETAVRITHIPTGVT+RCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGD VKAEWGQQIRNYVFHPYKLVKDVRTG+ETSDI SVMDGELD FIK YLKYK+S++ S
Sbjct:  287 QTSFSGVEVMPLLPEESLNVEIPEEDLEITFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDVVKAEWGQQIRNYVFHPYKLVKDVRTGYETSDISSVMDGELDPFIKTYLKYKYSMSQS 445