g.barbadense_cottongen_reftransV1_0094087, g.barbadense_cottongen_reftransV1_0094087 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy Swiss-Prot
Match: 4CLL1_ORYSJ (4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica GN=4CLL1 PE=2 SV=2)

HSP 1 Score: 392.889 bits (1008), Expect = 3.337e-125
Identity = 214/496 (43.15%), Postives = 306/496 (61.69%), Query Frame = -3

Query:  381 TYAEVHLTARKVATGLNN-LGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAK-----DNDIKIVTIDAPPEGC--LHFSELTEANEDEIPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIA-----QQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARL 1829
            T+AE+       A  L++  G++ GD ++LL  N   +   F   +  GA+ TT NP YTP EIAKQ + ++ KL+IT +    K+          D+D        PP+    L+ + +    E +     I   D  AL YSSGTTG  KGV+LTH++ + +       Q    E PN++       LC LP+FHI+ L+ +    L  G AI+ M +F+I +LME V++++VT    VPP+++A++K     KYDLSS++ + SG AP+GK++ + V  + P++++ QGYGMTE   ++++      + RE   G+ GT+V   E KIVD  T   LP NQ GEIC+RG  +M+GY N+ +AT+ TI K GWLHTGD+GY D   +LF+VDRLKELIKYKGFQ+APAELE +L++HP I DA V+P  D  AGEVP+A+VVRS  S +TE D+++FI KQV +YKRL RV F  ++PKS SGKILR+ L A++
Sbjct:   62 TFAELRSAVLSTAVALSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQVSDARAKLVITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLVAGVKEADYRRPPIKQSDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVF-------LCFLPMFHIFGLSVITYAQLHRGNAIIAMSRFDINSLMEAVQRHRVTHLFCVPPVIIALAKHGKAGKYDLSSLKFIGSGAAPLGKDVMEVVAKKFPDSEIVQGYGMTETCGIISLEYPEKGQAREF--GSTGTLVSGVEAKIVDIKTLKHLPPNQVGEICVRGPNVMQGYFNNVQATEFTI-KQGWLHTGDLGYFDGGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDAKAGEVPIAYVVRSPDSSLTEVDVQKFIEKQVAYYKRLKRVTFVGSVPKSASGKILRRQLIAQV 547          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy Swiss-Prot
Match: 4CLL9_ORYSJ (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 383.259 bits (983), Expect = 1.849e-121
Identity = 203/531 (38.23%), Postives = 314/531 (59.13%), Query Frame = -3

Query:  390 FRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTG-KIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPE--GCLHFSELTEANEDE----IPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLS--SIRMVMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLR 1955
            FRS  P + +P+ + +  +  +    + DR  L+    G + YTY EV     + A  L ++G+++G V+++ L N   +    LG    GA+ +  NP    AEI KQ   S+ KL++   V  +KVK    D  + ++ +       G + +  L  A +      +P       D+ ALPYSSGTTG+ KGVML+H++LV+++   +    P      + V L ++P FHIY +  +   +LR    +++M +F++ T +  +  ++V  AP VPP++LA+ KSPV D++DLS  +++ VM+  AP+  +L  A + + P  ++ + YG+TE   +   + A        K  + G ++ N E+K VDP TG SLP N  GE+C+R   +M+GY    E T+ T+D  GWLHTGD+GYID D ++FIVDR+KELIKYKGFQVAPAELEA+L++HP++ DA V  + DE AGEVPVA VVR +G++  E++I  +++++V  YKR+  +   DAIPKS SGKILR+ LR
Sbjct:   20 FRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTARFARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSEAKLVVANEVAFDKVK----DAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSDLCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETA--GQVVTLGLMPFFHIYGITGICCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLALKSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGFILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVGYIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVACVVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLR 544          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy Swiss-Prot
Match: 4CLL1_ARATH (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 376.711 bits (966), Expect = 4.399e-119
Identity = 212/541 (39.19%), Postives = 330/541 (61.00%), Query Frame = -3

Query:  381 MEADQHQHVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCLHFSELTEANED--EIPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRM--VMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARL 1991
            ME+ + +  E+IFRS  P + IP+ L L  +  + V ++ +    +   TGK  TY +V    +++A  L +LG+++G V++++L N  E+    LG    G + + ANP    +EI KQ  AS  + +IT A   EKVKS        IV  +   EG +++ +L EA +   +    +I   D+ ALP+SSGTTGL KGVMLTH++L+ ++   + G    +    + V L ++P FHIY +  +   +++    ++ M ++++   +  +  ++V+ AP VPPI+L + K+P++D++DLS +++  VM+  AP+  EL  A   + PN ++ + YG+TE   +   +    K     K  + G ++ N E+K +DP TG SLP+N SGE+C+R   +M+GY  + E T  TID+ GWLHTGDIGYIDDD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP++ D  VVP+ DE AGE+P A VV +  +   E+DI  F++  V  YK++  V F D+IPKS SGKI+R+ LR ++
Sbjct:    1 MESQKQEDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRLAKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGARGIITDATNYEKVKSLGLP---VIVLGEEKIEGAVNWKDLLEAGDKCGDTDNEEILQTDLCALPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMI--GQIVTLGLIPFFHIYGIVGICCATMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSVMTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILPNLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVINPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKI 536          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy Swiss-Prot
Match: 4CL2_DICDI (Probable 4-coumarate--CoA ligase 2 OS=Dictyostelium discoideum GN=4cl2 PE=3 SV=1)

HSP 1 Score: 376.711 bits (966), Expect = 5.442e-119
Identity = 219/551 (39.75%), Postives = 320/551 (58.08%), Query Frame = -3

Query:  390 EFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQ-ATASKTKLLITQAVYAEKVKSFAKDNDIK--------IVTIDAPPEG--------------CLHFSELTEANEDEIPAVKIN-SDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNAKL-GQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRL-GRVFFTDAIPKSPSGKILRKDLR 1964
            +  F S  P+I IP   P+     +++    D+  L++G T K Y+   V  T  KVA GLN L I++GDV+ ++L N PE++  F G    G I++  NP YT  E++   AT S   L +T AVY EK+K     ND+K        ++ +D   +                + F++L   N  + P V+I+   D   +P+SSGTTGL KGV L+H +LV++  Q    E  N  + + D ++  LP FHIY L + L+  ++ G  ++I+ KFE V  ++L++KYKV I+  VPPI +  +KSP++DK+DLSS+R + SG AP+ +E+ED +++R     +  QGYG TE  P       F       KSG+ G ++ N  +KI+ P TG +L   + GEICI+G  +M GY N+ +AT   IDKDG+  TGDIGY+D+D   FIVDR KELIK KGFQV PAELEA+L++HP ++DA VV +     GEVP  FVV      +TE ++  +   ++  YK   G +FF  AIPKS +GK+LRK+L+
Sbjct:    8 QIYFTSKYPNIIIPEK-PIPHLILKHIRSKPDQVLLVDGLTFKEYSSHFVADTIEKVACGLNKLNIKKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVY-EKIK-----NDLKRVFPSVEKVILVDIAGQTLKEIGQLTLSSDGIVMSFNQLINNNGKDYPIVRIDLKKDTAIIPFSSGTTGLFKGVCLSHHNLVSNTHQTQTVETTN--YKKNDTVMGQLPFFHIYGLMTYLILMVKQGHCVVILPKFEFVRFLDLIQKYKVAISFIVPPIAIMFAKSPIVDKFDLSSLRTLFSGAAPLSREVEDLIKERFKGKLIIKQGYGATELSPA-----CFVIPSGLIKSGSAGILLPNQLVKIISPETGENLGMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFFKTGDIGYVDEDGYYFIVDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLK 544          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy Swiss-Prot
Match: 4CL1_DICDI (Probable 4-coumarate--CoA ligase 1 OS=Dictyostelium discoideum GN=4cl1 PE=3 SV=1)

HSP 1 Score: 371.318 bits (952), Expect = 5.184e-117
Identity = 217/548 (39.60%), Postives = 317/548 (57.85%), Query Frame = -3

Query:  390 FRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQ-ATASKTKLLITQAVYAEKVKSFAKDNDIK--------IVTIDAPPEG--------------CLHFSELTEANEDEIPAVKIN-SDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNA-KLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRL-GRVFFTDAIPKSPSGKILRKDLR 1955
            F S  P+I IP   P+     +++    D+  L++G T K Y+   V  T  KVA GLN L I++GDV+ ++L N PE++  F G    G I++  NP YT  E++   AT S   L +T AVY EK+K     ND+K        ++ +D   +                + F++L   N  + P V+I+   D   +P+SSGTTGL KGV L+H ++V++  Q    E     + + D ++ +LP FHIY L   L+  ++ G  ++++ KFE V  +EL++KYKV I+  VPP+ +  +KSP++DK+DLSS+R + SG AP+  E+ED +++R      + QGYG TE  P       F       KSG+ G ++ N   KI+ P TG +L   + GEICI+G  +M GY N+ +AT   IDKDG+L TGDIGY+D+D   FIVDR KELIK KGFQV PAELEA+L++HP ++DA VV +     GEVP  FVV      +TE ++  +   ++  YK   G +FF  AIPKS +GK+LRK+L+
Sbjct:   11 FTSKYPNIIIPEK-PVPQLILKHIRSKPDQVLLVDGLTFKEYSSHFVADTIEKVACGLNKLNIKKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVY-EKIK-----NDLKRVFPSVEKVILVDIAGQTLKEIDQLTLSSDGIVMSFNQLINNNGKDYPIVRIDPKKDTAIIPFSSGTTGLFKGVCLSHHNIVSNTYQTQTIETST--YKKNDTVMGILPFFHIYGLMLFLMLMVKQGHRVVVLPKFEPVRFLELIQKYKVAISFIVPPVAIMFAKSPIVDKFDLSSLRTLFSGAAPLSSEVEDLIKERFKGRLVIKQGYGATELSPA-----CFVIPSGLVKSGSAGILLPNQLAKIISPETGENLGMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFLKTGDIGYVDEDGYYFIVDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLK 544          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy TrEMBL
Match: A0A0B0MBN2_GOSAR (4-coumarate--CoA ligase 1 OS=Gossypium arboreum GN=F383_02233 PE=4 SV=1)

HSP 1 Score: 1103.2 bits (2852), Expect = 0.000e+0
Identity = 534/543 (98.34%), Postives = 538/543 (99.08%), Query Frame = -3

Query:  363 MEADQHQHVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCLHFSELTEANEDEIPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARLAADVAN 1991
            MEADQHQH EFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQN PEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVT+DAPPEGCLHFSELTEANEDEIPAVKIN DDVVALPYSSGTTGLPKGVMLTHKSLVTS+AQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSG APMGKELEDA+RDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDP+TGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDA VVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARLAADVAN
Sbjct:    1 MEADQHQHDEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNLPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTVDAPPEGCLHFSELTEANEDEIPAVKINPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGAAPMGKELEDAIRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPHTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARLAADVAN 543          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy TrEMBL
Match: A0A0D2UDX2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G005900 PE=4 SV=1)

HSP 1 Score: 1093.95 bits (2828), Expect = 0.000e+0
Identity = 530/538 (98.51%), Postives = 534/538 (99.26%), Query Frame = -3

Query:  378 MEADQHQHVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCLHFSELTEANEDEIPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARLA 1991
            MEADQHQ VEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFL ASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCL+FSELTEANEDEIPAVKIN DDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDA RDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDP+TGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGK+LRKDLRA+LA
Sbjct:    1 MEADQHQRVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLAASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCLNFSELTEANEDEIPAVKINPDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAARDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPHTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKVLRKDLRAKLA 538          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy TrEMBL
Match: A0A061GPP7_THECC (4-coumarate:CoA ligase 2 OS=Theobroma cacao GN=TCM_038994 PE=4 SV=1)

HSP 1 Score: 985.712 bits (2547), Expect = 0.000e+0
Identity = 486/541 (89.83%), Postives = 516/541 (95.38%), Query Frame = -3

Query:  369 MEADQHQHVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCLHFSELTEANEDEIPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARLAADV 1991
            MEA+Q Q  EFIFRS LPDIYIP HLPLHTYCFE +SQFKDRPCLINGP G+IY+YAEV LTAR+VATGL NLGIQQGDVIMLLLQNSPEF+FAFLGASFRGAISTTANPFYTPAEI KQATASKT+LLITQAVYAEKVK+FAK+NDIKI+TID PPEGC+HFSELT+A+E EIPAVKIN DDVVALPYSSGTTGLPKGVMLTHKSLVTS+AQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELV+ YKVTIAPFVPPIVLA++K+P +DKYDLSSIR VMSG APMGKELEDAVR RLPNAKLGQGYGMTEAGPVLAMNLAFAKE  ETKSG+CGTVVRNAEMKIV+P TGASLP+NQSGEICIRG+QIMKGYLND EAT+ TIDKDGWLHTGDIGYIDDDDE+FIVDRLKELIKYKGFQVAPAELEAMLI HPNISDA VVPMKDEAAGEVPVAFVVRSNGSKITED+IKQFISKQVVFYKRLG+VFFT+AIPK+PSGKILRKDLRARLAAD+
Sbjct:    1 MEANQVQQ-EFIFRSRLPDIYIPKHLPLHTYCFEKISQFKDRPCLINGPNGRIYSYAEVELTARRVATGLKNLGIQQGDVIMLLLQNSPEFVFAFLGASFRGAISTTANPFYTPAEIVKQATASKTRLLITQAVYAEKVKNFAKENDIKIITIDTPPEGCMHFSELTQADESEIPAVKINPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVQNYKVTIAPFVPPIVLAIAKTPDVDKYDLSSIRTVMSGAAPMGKELEDAVRARLPNAKLGQGYGMTEAGPVLAMNLAFAKEACETKSGSCGTVVRNAEMKIVNPDTGASLPRNQSGEICIRGSQIMKGYLNDPEATQRTIDKDGWLHTGDIGYIDDDDEVFIVDRLKELIKYKGFQVAPAELEAMLIGHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQFISKQVVFYKRLGQVFFTEAIPKAPSGKILRKDLRARLAADI 540          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy TrEMBL
Match: A0A067KVV8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08679 PE=4 SV=1)

HSP 1 Score: 971.844 bits (2511), Expect = 0.000e+0
Identity = 464/538 (86.25%), Postives = 500/538 (92.94%), Query Frame = -3

Query:  378 MEADQHQHVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCLHFSELTEANEDEIPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARLA 1991
            ME       +FIFRS LPDIYIPNHLPLHTYCFEN+SQFKD PCLING TG+IYTYA+V LT+RKVA GL+ LGIQQGDVIMLLLQNSPEF+FAFLGASFRGAISTTANPFYTPAEI KQ TASK +L+ITQAVYAEKVK FAKD+DIK++TID+PPE CLHFSELT  +E++IP VKINSDDVVALPYSSGTTG PKGVMLTHK LVTS+AQQVDGENPNLYFHEKDVILC+LPLFHIYSLNSVLLC LRVGAAILIMQKFEI +LMELV KYKVTIAPFVPPIVLA++KSPV+DKYDLSSIR VMSG APMGKELEDAVR +LPNAKLGQGYGMTEAGPVL+M LAFAKEP E KSGACGTVVRNAEMKIVDP TG SLP+NQ+GEICIRG+QIMKGYLND EAT+ TIDKDGWLHTGD+GYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDA VVPMKDEAAGE+PVAFVVRSNGSKITED+IKQ+ISKQV+FYKR+ +VFFTDAIPK+PSGKILRKDLRARLA
Sbjct:    1 METKLDNQEDFIFRSKLPDIYIPNHLPLHTYCFENISQFKDNPCLINGATGEIYTYADVELTSRKVAAGLDKLGIQQGDVIMLLLQNSPEFVFAFLGASFRGAISTTANPFYTPAEIKKQLTASKAQLVITQAVYAEKVKQFAKDSDIKVMTIDSPPEDCLHFSELTSCDENDIPTVKINSDDVVALPYSSGTTGFPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCLLPLFHIYSLNSVLLCGLRVGAAILIMQKFEINSLMELVTKYKVTIAPFVPPIVLAIAKSPVVDKYDLSSIRTVMSGAAPMGKELEDAVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPDTGNSLPRNQAGEICIRGSQIMKGYLNDPEATERTIDKDGWLHTGDVGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEIPVAFVVRSNGSKITEDEIKQYISKQVIFYKRISKVFFTDAIPKAPSGKILRKDLRARLA 538          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy TrEMBL
Match: A0A0D2TGX0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G005900 PE=4 SV=1)

HSP 1 Score: 957.592 bits (2474), Expect = 0.000e+0
Identity = 464/470 (98.72%), Postives = 466/470 (99.15%), Query Frame = -3

Query:  582 MEADQHQHVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCLHFSELTEANEDEIPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVP 1991
            MEADQHQ VEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFL ASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCL+FSELTEANEDEIPAVKIN DDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDA RDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDP+TGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVP
Sbjct:    1 MEADQHQRVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLAASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCLNFSELTEANEDEIPAVKINPDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAARDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPHTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVP 470          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy TrEMBL
Match: T1RK64_9ROSI (Putative 4-coumarate:CoA ligase OS=Hibiscus cannabinus GN=4CL PE=2 SV=1)

HSP 1 Score: 956.051 bits (2470), Expect = 0.000e+0
Identity = 459/543 (84.53%), Postives = 499/543 (91.90%), Query Frame = -3

Query:  363 MEADQHQHVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCLHFSELTEANEDEIPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARLAADVAN 1991
            MEA+Q  H EFIFRS+LPDI IPNHLPLHTYCFEN+S FKD PCLIN PTG++YTYA+VHLT RKVA GLN LGIQQGDVIMLLL NSPEF+FAFLGASFRGAI+TTANPF+TPAEIAKQA+ASKT+L ITQAVYAEKVK+FA D DIKI+TID  PEGCLHFSELT  +EDEIPAVKIN DDVVALP+SSGTTGLPKGVMLTHKSLVTS+AQ V G+NPN+YFHE+DVILC+LPLFHIYSLN +LLCSLR GAAILIMQKFEI+ LMELVEKY VTIAPFVPPI+LA++K+P I KYDLSSIRMV+SG APMGK+LEDAVRDRLPNAKLGQGYGMTE   VLA+NLAFAKEP ETKSGACGTVVRNAEMKIVDP TG SLP+NQSGEICIRG+QIMKGYLND EATK TIDK+GWLHTGDIGYID+D+ELFIVDRLKELIKYKGFQVAPAELEAMLI+HPNISDA VVPMKDEAAGEVPVAF+VRSN S ITED+IKQFISKQVVFYKRL RVFF D IPK+PSGKILRKD+RA+LAA V N
Sbjct:    1 MEANQDGH-EFIFRSSLPDINIPNHLPLHTYCFENLSNFKDGPCLINAPTGRVYTYAQVHLTCRKVAAGLNKLGIQQGDVIMLLLHNSPEFVFAFLGASFRGAITTTANPFFTPAEIAKQASASKTRLFITQAVYAEKVKNFALDKDIKIITIDTTPEGCLHFSELTRVHEDEIPAVKINPDDVVALPFSSGTTGLPKGVMLTHKSLVTSVAQHVGGDNPNIYFHERDVILCLLPLFHIYSLNCILLCSLRAGAAILIMQKFEILPLMELVEKYSVTIAPFVPPIILAIAKTPDIQKYDLSSIRMVISGAAPMGKKLEDAVRDRLPNAKLGQGYGMTET--VLALNLAFAKEPWETKSGACGTVVRNAEMKIVDPETGTSLPRNQSGEICIRGSQIMKGYLNDPEATKRTIDKEGWLHTGDIGYIDEDNELFIVDRLKELIKYKGFQVAPAELEAMLISHPNISDAAVVPMKDEAAGEVPVAFIVRSNHSNITEDEIKQFISKQVVFYKRLARVFFVDTIPKAPSGKILRKDVRAKLAAHVPN 540          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy TrEMBL
Match: O48869_9ROSI (4-coumarate:CoA ligase 1 OS=Populus trichocarpa x Populus deltoides GN=4CL1 PE=2 SV=1)

HSP 1 Score: 954.896 bits (2467), Expect = 0.000e+0
Identity = 458/539 (84.97%), Postives = 500/539 (92.76%), Query Frame = -3

Query:  378 MEADQHQHVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDN-DIKIVTIDAPPEGCLHFSELTEANEDEIPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARLA 1991
            MEA   Q  EFIFRS LPDI+IPNHLPLHTYCFEN+S+FKD PCLINGPTG+I+TYAEV LT+RKVA+GLN LGI+QGDVI+LLLQNSPEF+FAFLGAS  GAISTTANPFYTPAE+AKQATASK KL+ITQAVYAEKV+ F K+N  +KIVT+D+PPE  LHFSELT ++ED+IPAV+IN DDVVALPYSSGTTGLPKGVMLTHK LVTS+AQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLC LRVG+AIL+MQKFEIVTLMELV+KYKVTIAPFVPP+VLAV+K PV+DKYDLSSIR VMSG APMGKELED VR +LPNAKLGQGYGMTEAGPVL+M LAFAKEP E KSGACGTVVRNAEMKIVDP TG SLP+NQSGEICIRG+QIMKGYLND EAT+ T+D DGWLHTGDIGYID DDELFIVDRLKELIKYKGFQVAPAELEAMLIAHP+ISD  VVPMKDEAAGEVP+AFVVR+NGSKITED+IKQ+ISKQVVFYKR+ RVFFT+AIPK+PSGKILRKDLRARLA
Sbjct:    1 MEAKNDQAQEFIFRSKLPDIHIPNHLPLHTYCFENLSRFKDNPCLINGPTGEIHTYAEVELTSRKVASGLNKLGIKQGDVILLLLQNSPEFVFAFLGASIIGAISTTANPFYTPAEVAKQATASKAKLIITQAVYAEKVQEFVKENVHVKIVTVDSPPENYLHFSELTNSDEDDIPAVEINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCGLRVGSAILLMQKFEIVTLMELVQKYKVTIAPFVPPVVLAVAKCPVVDKYDLSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPDTGRSLPRNQSGEICIRGSQIMKGYLNDPEATERTVDNDGWLHTGDIGYIDGDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPDISDCAVVPMKDEAAGEVPIAFVVRANGSKITEDEIKQYISKQVVFYKRISRVFFTEAIPKAPSGKILRKDLRARLA 539          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy TrEMBL
Match: D9ZKR6_9ROSI (4-coumarate:CoA ligase OS=Hibiscus cannabinus GN=4CL PE=2 SV=1)

HSP 1 Score: 951.429 bits (2458), Expect = 0.000e+0
Identity = 457/543 (84.16%), Postives = 497/543 (91.53%), Query Frame = -3

Query:  363 MEADQHQHVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCLHFSELTEANEDEIPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARLAADVAN 1991
            MEA+Q  H EFIFRS+LPDI IPNHLPLHTYCFEN+S FKD PCLIN PTG++YTYA+VHLT RKVA GLN LGIQQGDVIMLLL NSPEF+FAFLGASFRGAI+TTANPF+TPAEIAKQA+ASKT+L ITQAVYAEKVK+FA D DIKI+TID  PEGCLHFSELT  +EDEIPAVKIN DDVVALP+SSGTTGLPKGVMLTHKSLVTS+AQ V G+NPN+YFHE+DVILC+LPLFHIYSLN +LLCSLR GAAILIMQKFEI+ L ELVEKY VTIAPFVPPI+LA++K+P I KYDLSSIRMV+SG APMGK+LEDAVRDRLPNAKLGQGYGMTE   VLA+NLAFAKEP ETKSGACGTVVRNAEMKIVDP TG SLP+NQSGEICIRG+QIMKGYLND EATK TIDK+GWLHTGDIGYID+D+ELFIVDRLKELIKYKGFQVAPAELEAMLI+HPNISDA VVPMKDEAAGEVPVAF+VRSN S I ED+IKQFISKQVVFYKRL RVFF D IPK+PSGKILRKD+RA+LAA V N
Sbjct:    1 MEANQDGH-EFIFRSSLPDINIPNHLPLHTYCFENLSNFKDGPCLINAPTGRVYTYAQVHLTCRKVAAGLNKLGIQQGDVIMLLLHNSPEFVFAFLGASFRGAITTTANPFFTPAEIAKQASASKTRLFITQAVYAEKVKNFALDKDIKIITIDTTPEGCLHFSELTRVHEDEIPAVKINPDDVVALPFSSGTTGLPKGVMLTHKSLVTSVAQHVGGDNPNIYFHERDVILCLLPLFHIYSLNCILLCSLRAGAAILIMQKFEILPLTELVEKYSVTIAPFVPPIILAIAKTPDIQKYDLSSIRMVISGAAPMGKKLEDAVRDRLPNAKLGQGYGMTET--VLALNLAFAKEPWETKSGACGTVVRNAEMKIVDPETGTSLPRNQSGEICIRGSQIMKGYLNDPEATKRTIDKEGWLHTGDIGYIDEDNELFIVDRLKELIKYKGFQVAPAELEAMLISHPNISDAAVVPMKDEAAGEVPVAFIVRSNHSNIIEDEIKQFISKQVVFYKRLARVFFVDTIPKAPSGKILRKDVRAKLAAHVPN 540          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy TrEMBL
Match: V4U1F8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10007733mg PE=4 SV=1)

HSP 1 Score: 942.569 bits (2435), Expect = 0.000e+0
Identity = 463/594 (77.95%), Postives = 516/594 (86.87%), Query Frame = -3

Query:  375 GGSSTITYLFISLNQTINQPDQTSQKQNQTLTFFFCLSQFS--PFSYFL*ISPLMEADQHQHVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDNDIKIVTIDAPPEGCLHFSEL-TEANEDEIPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARLAA 2147
            GG  +    F   + + + P   S K NQTLT  F    FS   FS+   +   MEA Q  H EFIFRS LPDIYIPNHLPLHTYCFEN+SQF DRPC+INGPTG +YTYA+V LT+RK A GL+ LG+Q+GDVIMLLLQN P+F+FAFLGAS+ GA STTANPFYTPAEIAKQA ASK K++ITQAVY +KV+  ++ +  +IVTID PPE CLHFSEL T+A+E+EIPAVKIN DDVVALPYSSGTTGLPKGVMLTH+ LVTS+AQQVDGENPNLY H++DVILCVLPLFHIYSLNSVLLC+LRVGAAILIMQKFEIV LMELV+KYKVT+APFVPPIVLAV+KS  +DKYD+SSIR VMSG APMGKELEDAVR +LP+AKLGQGYGMTEAGPVL+M LAFAKEP E KSGACGTVVRNAEMKIV P TG SLP+NQ+GEICIRG QIMKGYLND EAT+ TIDK+GWLHTGDIGYIDD+DELFIVDRLKELIK+KGFQVAPAELEAMLIAHPNISDA VVPMKDEAAGEVPVAFVVRSNGSKITED+IKQ+ISKQVVFYKR+ RVFFTD+IPK+PSGKILRKDLR +LAA
Sbjct:   39 GGLQSKLMTFTHHSLSPSTPSHGS-KNNQTLTIPFASQNFSLSHFSHIHILFAKMEAKQ-DHQEFIFRSKLPDIYIPNHLPLHTYCFENISQFADRPCIINGPTGDVYTYADVELTSRKFAAGLSKLGVQKGDVIMLLLQNCPQFVFAFLGASYIGATSTTANPFYTPAEIAKQAIASKAKIIITQAVYNDKVRDLSEHHGARIVTIDPPPENCLHFSELITQADENEIPAVKINPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYLHKEDVILCVLPLFHIYSLNSVLLCALRVGAAILIMQKFEIVKLMELVQKYKVTVAPFVPPIVLAVAKSGDVDKYDMSSIRTVMSGAAPMGKELEDAVRAKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVVRNAEMKIVHPDTGISLPRNQAGEICIRGAQIMKGYLNDREATETTIDKEGWLHTGDIGYIDDEDELFIVDRLKELIKFKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVVFYKRINRVFFTDSIPKAPSGKILRKDLRTKLAA 630          

BLAST of g.barbadense_cottongen_reftransV1_0094087 vs. ExPASy TrEMBL
Match: B9IM06_POPTR (4-coumarate-CoA ligase 2 family protein OS=Populus trichocarpa GN=POPTR_0018s10210g PE=4 SV=2)

HSP 1 Score: 937.176 bits (2421), Expect = 0.000e+0
Identity = 450/540 (83.33%), Postives = 497/540 (92.04%), Query Frame = -3

Query:  375 MEADQHQHVEFIFRSTLPDIYIPNHLPLHTYCFENVSQFKDRPCLINGPTGKIYTYAEVHLTARKVATGLNNLGIQQGDVIMLLLQNSPEFIFAFLGASFRGAISTTANPFYTPAEIAKQATASKTKLLITQAVYAEKVKSFAKDND-IKIVTIDAPPEGCLHFSELTEANEDEIPAVKINSDDVVALPYSSGTTGLPKGVMLTHKSLVTSIAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSVLLCSLRVGAAILIMQKFEIVTLMELVEKYKVTIAPFVPPIVLAVSKSPVIDKYDLSSIRMVMSGGAPMGKELEDAVRDRLPNAKLGQGYGMTEAGPVLAMNLAFAKEPRETKSGACGTVVRNAEMKIVDPYTGASLPQNQSGEICIRGTQIMKGYLNDTEATKVTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDATVVPMKDEAAGEVPVAFVVRSNGSKITEDDIKQFISKQVVFYKRLGRVFFTDAIPKSPSGKILRKDLRARLAA 1991
            MEA++ Q  EFIFRS LPDIYIPNHLPLHTYCFE +SQFKD PCLINGPTG IYTYA+V LT+RKVA+GL  LG+QQGDVI+LLLQNSPEF+FA LGASF GAIS+TANPFYT AEIAKQATASK KL+ITQA YAEKV+ FA++ND +KI+TID+  E CLHFSELT ++E+EIPAVKI  DDVVALPYSSGTTGLPKGVMLTHK LVTS+AQQVDGENPNLYFHE+DVILCVLPLFHIYSLNSVLLC LR G+AIL+MQKFE V+LM+LV+KYKVTIAP VPPI LA++KSPV+D+YDLSSIR V+SG APMGKELED VR +LPNAKLGQGYGMTEAGPV+AM LAFAKEP E KSGACGTVVRNAEMKIVDP TG S P+N++GEICIRG QIMKGYLNDTEAT+ TIDKDGWLHTGD+GYI D+DELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDA VVPMKDEAAGEVPVAFVVRSNGSKITED+IKQ+ISKQV+FYKR+GRVFFT+AIPK+PSGKILRKDLRAR+AA
Sbjct:    1 MEANKDQVQEFIFRSKLPDIYIPNHLPLHTYCFEKLSQFKDYPCLINGPTGDIYTYADVELTSRKVASGLYKLGVQQGDVILLLLQNSPEFVFALLGASFIGAISSTANPFYTSAEIAKQATASKAKLIITQAAYAEKVQQFAQENDHVKIMTIDSLTENCLHFSELTSSDENEIPAVKIKPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHERDVILCVLPLFHIYSLNSVLLCGLRAGSAILLMQKFETVSLMDLVQKYKVTIAPLVPPIFLAIAKSPVVDQYDLSSIRTVLSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVIAMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPETGDSQPRNKAGEICIRGCQIMKGYLNDTEATERTIDKDGWLHTGDVGYI-DEDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEVPVAFVVRSNGSKITEDEIKQYISKQVIFYKRIGRVFFTEAIPKAPSGKILRKDLRARVAA 539