g.barbadense_cottongen_reftransV1_0093436, g.barbadense_cottongen_reftransV1_0093436 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 4.759e-45
Identity = 61/133 (45.86%), Postives = 87/133 (65.41%), Query Frame = 1

Query:  937 LTL*NSLFQRSKSTAM-VFVA*GGKMNEIPETETPHPHRAGNIFVILYMVDLEEKENINSEKFMSWMRRVYNYMTPFITKSPREIYINYRDLDIGVNKFNNHKGNYAQARIWGLKYFKNNFNRLVHITTKLGP 1332
            +T+   L++      M VF   GG M+EI E+  P PHRAG  + I Y+   E++E+  +EK ++W+R VYN+ TP+++++PR  Y+NYRDLD+G   F +   NY QARIWG KYF  NFNRLV + TK+ P
Sbjct:  398 VTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEKQED--NEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFES-PNNYTQARIWGEKYFGKNFNRLVKVKTKVDP 527          

HSP 2 Score: 63.929 bits (154), Expect = 4.759e-45
Identity = 36/102 (35.29%), Postives = 58/102 (56.86%), Query Frame = 3

Query:  672 ERVKSSKDEDKMTIQARFSSMFLGGTDKLLQLMEEKFPQLGLMKEDCLEMSWAESDLYFEQF----PIGAPLETLLGRNHXXXXXXXXXXXXXDFVKQPIPE 965
              +  ++ ++K TI + FSS+F GG D L+ LM + FP+LG+ K DC ++SW ++ +++             E LL R+      K+ F  K D+VK+PIPE
Sbjct:  296 RNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRS---GGRKAAFSIKLDYVKKPIPE 394          

HSP 3 Score: 30.4166 bits (67), Expect = 4.759e-45
Identity = 23/81 (28.40%), Postives = 39/81 (48.15%), Query Frame = 2

Query:  449 KSIGEDMFWAIRXXXXXXXXALG*S*LGKQTRCQFQLMSPFSEFTECWIK--------TQLIHQWQYVAHKFPKEIHMSIH 667
            KS+GED+FWAIR          G    G     + +L++  S  T   +K         +L+++WQ +A+ + KE+ +  H
Sbjct:  220 KSMGEDLFWAIR--------GGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTH 292          

HSP 4 Score: 28.8758 bits (63), Expect = 4.759e-45
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 3

Query: 1335 NFFRHEQSIPPL 1370
            NFFR+EQSIPPL
Sbjct:  529 NFFRNEQSIPPL 540          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 6.435e-45
Identity = 61/133 (45.86%), Postives = 88/133 (66.17%), Query Frame = 1

Query:  937 LTL*NSLFQRSKSTAM-VFVA*GGKMNEIPETETPHPHRAGNIFVILYMVDLEEKENINSEKFMSWMRRVYNYMTPFITKSPREIYINYRDLDIGVNKFNNHKGNYAQARIWGLKYFKNNFNRLVHITTKLGP 1332
            +T+   L++      M VF   GG M+EI E+  P PHRAG ++ I Y+   E++E+  +EK ++W+R VYN+ TP+++++PR  Y+NYRDLD+G   F +   NY QARIWG KYF  NFNRLV + TK+ P
Sbjct:  398 VTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEKQED--NEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFES-PNNYTQARIWGEKYFGKNFNRLVKVKTKVDP 527          

HSP 2 Score: 62.7734 bits (151), Expect = 6.435e-45
Identity = 36/102 (35.29%), Postives = 58/102 (56.86%), Query Frame = 3

Query:  672 ERVKSSKDEDKMTIQARFSSMFLGGTDKLLQLMEEKFPQLGLMKEDCLEMSWAESDLYFEQFPIGAPL----ETLLGRNHXXXXXXXXXXXXXDFVKQPIPE 965
              +  ++ ++K TI + FSS+F GG D L+ LM + FP+LG+ K DC ++SW ++ +++             E LL R+      K+ F  K D+VK+PIPE
Sbjct:  296 RNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRS---GGRKAAFSIKLDYVKKPIPE 394          

HSP 3 Score: 30.0314 bits (66), Expect = 6.435e-45
Identity = 23/81 (28.40%), Postives = 39/81 (48.15%), Query Frame = 2

Query:  449 KSIGEDMFWAIRXXXXXXXXALG*S*LGKQTRCQFQLMSPFSEFTECWIK--------TQLIHQWQYVAHKFPKEIHMSIH 667
            KS+GED+FWAIR          G    G     + +L++  S  T   +K         +L+++WQ +A+ + KE+ +  H
Sbjct:  220 KSMGEDLFWAIR--------GGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTH 292          

HSP 4 Score: 28.8758 bits (63), Expect = 6.435e-45
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 3

Query: 1335 NFFRHEQSIPPL 1370
            NFFR+EQSIPPL
Sbjct:  529 NFFRNEQSIPPL 540          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 3.180e-44
Identity = 56/111 (50.45%), Postives = 80/111 (72.07%), Query Frame = 1

Query: 1000 GGKMNEIPETETPHPHRAGNIFVILYMVDLEEKENINSEKFMSWMRRVYNYMTPFITKSPREIYINYRDLDIGVNKFNNHKGNYAQARIWGLKYFKNNFNRLVHITTKLGP 1332
            GG M+EI E+  P PHRAG ++ + Y+   E++E+  +EK ++W+R +YN+MTP+++K+PR  Y+NYRDLDIG+N   N   NY QARIWG KYF  NF+RLV + T + P
Sbjct:  419 GGIMDEISESAIPFPHRAGILYELWYICSWEKQED--NEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKN-PNNYTQARIWGEKYFGKNFDRLVKVKTLVDP 526          

HSP 2 Score: 63.5438 bits (153), Expect = 3.180e-44
Identity = 36/106 (33.96%), Postives = 58/106 (54.72%), Query Frame = 3

Query:  672 ERVKSSKDEDKMTIQARFSSMFLGGTDKLLQLMEEKFPQLGLMKEDCLEMSWAESDLYF--------EQFPIGAPLETLLGRNHXXXXXXXXXXXXXDFVKQPIPE 965
              +  ++ ++K  I   FSS+FLGG D L+ LM + FP+LG+ K DC ++SW ++ +++        + F     L+   G+N         FK K D+VK+PIPE
Sbjct:  295 RNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSAGQN-------GAFKIKLDYVKKPIPE 393          

HSP 3 Score: 30.4166 bits (67), Expect = 3.180e-44
Identity = 17/62 (27.42%), Postives = 33/62 (53.23%), Query Frame = 1

Query:  427 LMEEFLIKIHWR---RYVLGHSRRWXXX---XXSFGVVLAWKTNSVPVPANVTVFRVHRMLD 594
            +++  L+ +H +   R  +G    W        SFG+++AWK   V VP + T+F V ++++
Sbjct:  204 IIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKKIME 264          

HSP 4 Score: 28.8758 bits (63), Expect = 3.180e-44
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 3

Query: 1332 QNFFRHEQSIPPL 1370
             NFFR+EQSIPPL
Sbjct:  527 NNFFRNEQSIPPL 539          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.306e-42
Identity = 56/113 (49.56%), Postives = 77/113 (68.14%), Query Frame = 1

Query: 1000 GGKMNEIPETETPHPHRAGNIFVILYMVDLEEKENINSEKFMSWMRRVYNYMTPFITKSPREIYINYRDLDIGVNKFNNHKG--NYAQARIWGLKYFKNNFNRLVHITTKLGP 1332
            GG M EI E+  P PHRAG ++ + Y    E++E+  +EK ++W+R VYN+ TP+++++PR  Y+NYRDLD+G     NH    NY QARIWG KYF  NFNRLV + TK+ P
Sbjct:  420 GGIMEEISESAIPFPHRAGIMYELWYTASWEKQED--NEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGK---TNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDP 527          

HSP 2 Score: 64.3142 bits (155), Expect = 1.306e-42
Identity = 45/136 (33.09%), Postives = 68/136 (50.00%), Query Frame = 3

Query:  570 FQSSQNVGSKRN*FISGSMLHTSFLKKYT*AST*ERVKSSKDEDKMTIQARFSSMFLGGTDKLLQLMEEKFPQLGLMKEDCLEMSWAESDLYFEQF----PIGAPLETLLGRNHXXXXXXXXXXXXXDFVKQPIPE 965
            F   QN+  K +      +L T F+ K         +  +  ++K T+   FSS+F GG D L+ LM + FP+LG+ K DC E SW ++ +++             E LL R+   A  K+ F  K D+VK+PIPE
Sbjct:  273 FNKWQNIAYKYD---KDLVLMTHFITK--------NITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRS---AGKKTAFSIKLDYVKKPIPE 394          

HSP 3 Score: 30.4166 bits (67), Expect = 1.306e-42
Identity = 11/28 (39.29%), Postives = 18/28 (64.29%), Query Frame = 1

Query:  511 FGVVLAWKTNSVPVPANVTVFRVHRMLD 594
            FG++ AWK   V VP+  T+F V + ++
Sbjct:  238 FGIIAAWKIKLVAVPSKSTIFSVKKNME 265          

HSP 4 Score: 28.8758 bits (63), Expect = 1.306e-42
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 3

Query: 1332 QNFFRHEQSIPPL 1370
             NFFR+EQSIPPL
Sbjct:  528 NNFFRNEQSIPPL 540          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy Swiss-Prot
Match: RETOL_ARATH (Reticuline oxidase-like protein OS=Arabidopsis thaliana GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 88.5817 bits (218), Expect = 2.112e-32
Identity = 48/119 (40.34%), Postives = 71/119 (59.66%), Query Frame = 1

Query:  979 AMVFVA*GGKMNEIPETETPHPHRAGNIFVILYMVDLEEKENINSEKFMSWMRRVYNYMTPFITKSPREIYINYRDLDIGVNKFNNHKGN-YAQARIWGLKYFKNNFNRLVHITTKLGP 1332
             +VF   GGKM E+     P PHR   +F I Y V+ +E      + +++  + +Y++MT F++K+PR  Y NYRD+DIGV   N+H  N Y +  ++G KYF  NF+RLV I T + P
Sbjct:  409 GLVFNPYGGKMAEVAVNAKPFPHR-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGV---NDHGANSYKEGEVYGRKYFGENFDRLVKIKTAVDP 523          

HSP 2 Score: 53.5286 bits (127), Expect = 2.112e-32
Identity = 34/91 (37.36%), Postives = 56/91 (61.54%), Query Frame = 3

Query:  708 TIQARFSSMFLGGTDKLLQLMEEKFPQLGLMKEDCLEMSWAESDLYFEQFPIGAPLE--TLLGRNHXXXXXXXXXXXXXDFVKQPIPEKQI 974
            T++A   ++FLG  D+++ L+ ++FP+LGL KE+C EM+W +S L+++       ++    L RN     + SF K KSD+V   IP+K I
Sbjct:  308 TVRASVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRN---LDTSSFGKRKSDYVATAIPKKGI 395          

HSP 3 Score: 30.4166 bits (67), Expect = 2.112e-32
Identity = 13/27 (48.15%), Postives = 18/27 (66.67%), Query Frame = 1

Query:  529 WKTNSVPVPANVTVFRVHRMLDQNATN 609
            +K   VPVP+ VTVFRV + +D  A +
Sbjct:  250 YKVKLVPVPSVVTVFRVEQYMDSGAVD 276          

HSP 4 Score: 25.7942 bits (55), Expect = 2.112e-32
Identity = 10/12 (83.33%), Postives = 11/12 (91.67%), Query Frame = 3

Query: 1335 NFFRHEQSIPPL 1370
            NFFR+EQSIP L
Sbjct:  525 NFFRNEQSIPTL 536          

HSP 5 Score: 23.8682 bits (50), Expect = 2.112e-32
Identity = 8/11 (72.73%), Postives = 11/11 (100.00%), Query Frame = 2

Query:  449 KSIGEDMFWAI 481
            K++GED+FWAI
Sbjct:  226 KAMGEDLFWAI 236          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy Swiss-Prot
Match: RETO_ESCCA (Reticuline oxidase OS=Eschscholzia californica GN=BBE1 PE=1 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 4.239e-29
Identity = 40/114 (35.09%), Postives = 65/114 (57.02%), Query Frame = 1

Query: 1000 GGKMNEIPETETPHPHRAGNIFVILYMVDLEEKENINSEKFMSWMRRVYNYMTPFITKSPREIYINYRDLDIGVNKFNNHK---GNYAQARIWGLKYFKNNFNRLVHITTKLGP 1332
            GG+M++I    TP PHR+G   ++ Y+V   + E     +F+ W+ +VY +M PF++K+PR  Y+N+ DLD+G   + N          +R WG  YF +N+ RL+   T + P
Sbjct:  393 GGQMSKISSDFTPFPHRSGTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDP 506          

HSP 2 Score: 41.5874 bits (96), Expect = 4.239e-29
Identity = 29/68 (42.65%), Postives = 37/68 (54.41%), Query Frame = 3

Query:  780 FPQLGLMKEDCLEMSWAESDLYFEQFPIGAPLETLLG-RNHXXXXXXXXXXXXXDFVKQPIPEKQIHG 980
            FP+LGL++ED LEMSW ES  Y       A LET+    N      +  FK K D  K+P+P K  +G
Sbjct:  313 FPELGLVEEDYLEMSWGESFAYL------AGLETVSQLNNRFLKFDERAFKTKVDLTKEPLPSKAFYG 374          

HSP 3 Score: 33.113 bits (74), Expect = 4.239e-29
Identity = 13/25 (52.00%), Postives = 17/25 (68.00%), Query Frame = 1

Query:  511 FGVVLAWKTNSVPVPANVTVFRVHR 585
            +G + AWK   +PVP  VTVFRV +
Sbjct:  228 WGAIYAWKIKLLPVPEKVTVFRVTK 252          

HSP 4 Score: 26.9498 bits (58), Expect = 4.239e-29
Identity = 8/22 (36.36%), Postives = 16/22 (72.73%), Query Frame = 2

Query:  599 TQLIHQWQYVAHKFPKEIHMSI 664
            T L+H+WQ+VA +  ++  +S+
Sbjct:  260 TSLLHKWQFVAEELEEDFTLSV 281          

HSP 5 Score: 23.483 bits (49), Expect = 4.239e-29
Identity = 8/13 (61.54%), Postives = 9/13 (69.23%), Query Frame = 3

Query: 1332 QNFFRHEQSIPPL 1370
             N F H QSIPP+
Sbjct:  507 NNVFNHPQSIPPM 519          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 6.424e-26
Identity = 56/112 (50.00%), Postives = 77/112 (68.75%), Query Frame = 1

Query: 1000 GGKMNEIPETETPHPHRAGNIFVILYMVDLEEKENINSEKFMSWMRRVYNYMTPFITKSPREIYINYRDLDIGVNKFN-NHKGNYAQARIWGLKYFKNNFNRLVHITTKLGP 1332
            GG M+EI E+  P PHRAG ++ + Y    E++E+  +EK ++W+R VYN+ TP+++++PR  Y+NYRDLD+G  K N     NY QARIWG KYF  NFNRLV + TK  P
Sbjct:  420 GGIMDEISESAIPFPHRAGIMYELWYTATWEKQED--NEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLG--KTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADP 527          

HSP 2 Score: 29.261 bits (64), Expect = 6.424e-26
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 3

Query: 1332 QNFFRHEQSIPPL 1370
             NFFR+EQSIPPL
Sbjct:  528 NNFFRNEQSIPPL 540          

HSP 3 Score: 62.7734 bits (151), Expect = 2.389e-13
Identity = 23/60 (38.33%), Postives = 39/60 (65.00%), Query Frame = 3

Query:  672 ERVKSSKDEDKMTIQARFSSMFLGGTDKLLQLMEEKFPQLGLMKEDCLEMSWAESDLYFE 851
              +  +  ++K T+   FSS+FLGG D L+ LM + FP+LG+ K DC E+SW ++ +++ 
Sbjct:  296 RNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYS 355          

HSP 4 Score: 35.4242 bits (80), Expect = 2.389e-13
Identity = 25/87 (28.74%), Postives = 42/87 (48.28%), Query Frame = 2

Query:  449 KSIGEDMFWAIRXXXXXXXXALG*S*LGKQTRCQFQLMSPFSEFTECWIK--------TQLIHQWQYVAHKFPKEIHMSIHMRKGEI 685
            KS+GED+FWAIR          G    G     + +L+   S+ T   +K         +L ++WQ +A+K+ K++ ++ H R   I
Sbjct:  220 KSMGEDLFWAIR--------GGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNI 298          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy Swiss-Prot
Match: PHI_MYCGS (3-hexulose-6-phosphate isomerase OS=Mycobacterium gastri GN=rmpB PE=2 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.113e-20
Identity = 66/196 (33.67%), Postives = 98/196 (50.00%), Query Frame = -2

Query: 1421 NGSMASLASQICNDIASIFSKPSIHPPALD-LMVAELASTVSQKGRVFLYGVGREGLMLKALCMRLAHLGLSAHFVFDMTTPPITSKDLLVASAGPGGFSTVDAICSVARSHGGRVLLLTAQPETGSSVRHANVVAYVAAQTMADDGGGEKSRTLLPMGSVYEGAMFVLFEMVVYKLGEVLGESPEAIRARHTNLE 2005
            +G++  +   I N+++ +  + +     +D   VA LA  + Q GRVF+ G GR GL+L+   MRL H GL+ H   D TTP I++ DLL+ ++G G  S V      A+  G R+   T  P++  +     VV   AAQ    D G   SR     GS++E  +FV+ E V   L +      E +  RH NLE
Sbjct:    8 DGAVKVVGDDITNNLSLVRDEVADTAAKVDPEQVAVLARQIVQPGRVFVAGAGRSGLVLRMAAMRLMHFGLTVHVAGDTTTPAISAGDLLLVASGSGTTSGVVKSAETAKKAGARIAAFTTNPDSPLAGLADAVVIIPAAQKT--DHGSHISRQY--AGSLFEQVLFVVTEAVFQSLWDHTEVEAEELWTRHANLE 199          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy Swiss-Prot
Match: PHI_BACSU (3-hexulose-6-phosphate isomerase OS=Bacillus subtilis (strain 168) GN=hxlB PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.586e-19
Identity = 53/161 (32.92%), Postives = 85/161 (52.80%), Query Frame = -2

Query: 1421 ELASTVSQKGRVFLYGVGREGLMLKALCMRLAHLGLSAHFVFDMTTPPITSKDLLVASAGPGGFSTVDAICSVARSHGGRVLLLTAQPETGSSVRHANVVAYVAAQTMADDGGGEKSRTLLPMGSVYEGAMFVLFEMVVYKLGEVLGESPEAIRARHTNLE 1903
            +LA  +    ++F  G GR GLM K+  MRL H+G +AH V ++ TPP+   DL++  +G G   ++    + A+S  G V  LT  PE+ S  + A+++  +         G  K  T+ PMGS++E  + + ++ V+ KL E  G   E +   H NLE
Sbjct:   28 QLADHILSSHQIFTAGAGRSGLMAKSFAMRLMHMGFNAHIVGEILTPPLAEGDLVIIGSGSGETKSLIHTAAKAKSLHGIVAALTINPES-SIGKQADLIIRMPGSPKDQSNGSYK--TIQPMGSLFEQTLLLFYDAVILKLMEKKGLDSETMFTHHANLE 185          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy Swiss-Prot
Match: RETO_PAPSO (Reticuline oxidase OS=Papaver somniferum GN=BBE1 PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 4.404e-17
Identity = 43/114 (37.72%), Postives = 62/114 (54.39%), Query Frame = 1

Query: 1000 GGKMNEIPETETPHPHRAGNIFVILYMVDLEEKENINSEKFMSWMRRVYNYMTPFITKSPREIYINYRDLDIGVNKFNNHKGNYAQ---ARIWGLKYFKNNFNRLVHITTKLGP 1332
            GGKM+EI    TP PHR G   +  Y++   + E     +F  W+ + Y+Y+ PF++K PR  Y+N+ DLDIG   + N          AR WG +YF +N+ RLV   T + P
Sbjct:  397 GGKMSEISTDFTPFPHRKGTKLMFEYIIAWNQDEESKIGEFSEWLAKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWRNKSSTTNAVEIARNWGERYFSSNYERLVKAKTLIDP 510          

HSP 2 Score: 25.0238 bits (53), Expect = 4.404e-17
Identity = 8/14 (57.14%), Postives = 10/14 (71.43%), Query Frame = 3

Query: 1332 QNFFRHEQSIPPLL 1373
             N F H QSIPP++
Sbjct:  511 NNVFNHPQSIPPMM 524          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy TrEMBL
Match: B9ID53_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0015s13430g PE=4 SV=2)

HSP 1 Score: 302.753 bits (774), Expect = 2.808e-94
Identity = 155/205 (75.61%), Postives = 174/205 (84.88%), Query Frame = -2

Query: 1421 MDTGNGSMASLASQICNDIASIFSKPS-IHPPALDLMVAELASTVSQKGRVFLYGVGREGLMLKALCMRLAHLGLSAHFVFDMTTPPITSKDLLVASAGPGGFSTVDAICSVARSHGGRVLLLTAQPETGSSVRHANVVAYVAAQTMA-----DDGGGEKSRTLLPMGSVYEGAMFVLFEMVVYKLGEVLGESPEAIRARHTNLE 2017
            M T   SM+SLAS+ICN +ASIF+KP+  HPP LDLM+  L ST +Q GRVFLYGVGREGLMLKALCMRLAHLG+S HFVFDMTTPPITS DLL+ASAGPGGFSTVDA+CS+ARS+G  VLLLTAQPETGS V+HA+VV YV AQTMA      DGG EKSR L+PMGSV+EGA+FV FEMVVYKLGE LG+SPE IR+RHTNLE
Sbjct:    1 MGTETPSMSSLASRICNHMASIFAKPTGPHPPPLDLMITTLTSTAAQNGRVFLYGVGREGLMLKALCMRLAHLGISTHFVFDMTTPPITSNDLLIASAGPGGFSTVDALCSLARSYGATVLLLTAQPETGSCVKHASVVCYVPAQTMADDKGDGDGGEEKSRPLMPMGSVFEGALFVFFEMVVYKLGEALGQSPEVIRSRHTNLE 205          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy TrEMBL
Match: A0A072VQM0_MEDTR (3-hexulose-6-phosphate isomerase, putative OS=Medicago truncatula GN=MTR_1g100753 PE=4 SV=1)

HSP 1 Score: 299.286 bits (765), Expect = 5.847e-93
Identity = 153/195 (78.46%), Postives = 174/195 (89.23%), Query Frame = -2

Query: 1421 MASLASQICNDIASIFSKPS-IHPPALDLMVAELASTVSQKGRVFLYGVGREGLMLKALCMRLAHLGLSAHFVFDMTTPPITSKDLLVASAGPGGFSTVDAICSVARSHGGRVLLLTAQPETGSSVRHANVVAYVAAQTMADDGGGE--KSRTLLPMGSVYEGAMFVLFEMVVYKLGEVLGESPEAIRARHTNLE 1996
            MAS+A+QICN I+SIFS P+  +PP LDL++ EL++  SQ GRVFLYGVGREGLMLKA CMRLAHLGLSAH VFDMTTPPIT+ DLL+ASAGPGGFSTVDAICSVARS+GGRVLLLTAQPETGS V+HA+VVAYV A+TMA+D   E  KSR LLPMGSVYEGA+FVLFEM VYKLG++LGESPEA+R+RHTNLE
Sbjct:   12 MASVATQICNQISSIFSTPTHPYPPPLDLLLTELSTISSQNGRVFLYGVGREGLMLKAFCMRLAHLGLSAHLVFDMTTPPITTNDLLIASAGPGGFSTVDAICSVARSNGGRVLLLTAQPETGSCVKHASVVAYVPAKTMANDKDEEDVKSRPLLPMGSVYEGALFVLFEMAVYKLGDILGESPEAVRSRHTNLE 206          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy TrEMBL
Match: A0A0A0LBC8_CUCSA (3-hexulose-6-phosphate isomerase OS=Cucumis sativus GN=Csa_3G181970 PE=4 SV=1)

HSP 1 Score: 296.204 bits (757), Expect = 9.715e-92
Identity = 145/197 (73.60%), Postives = 170/197 (86.29%), Query Frame = -2

Query: 1421 SMASLASQICNDIASIFSKPSI-HPPALDLMVAELASTVSQKGRVFLYGVGREGLMLKALCMRLAHLGLSAHFVFDMTTPPITSKDLLVASAGPGGFSTVDAICSVARSHGGRVLLLTAQPETGSSVRHANVVAYVAAQTMADD---GGGEKSRTLLPMGSVYEGAMFVLFEMVVYKLGEVLGESPEAIRARHTNLE 1999
            S ASLAS+IC++IASIFSKP+  + P L+++V+EL+S   +K RVFLYGVGREGLMLKALCMRLAHLGLS+H VFDM  PPI   DLL+ASAGPGGFSTVDAICSVARS GGRVL+LTAQPE GSSV++A+V+ YV AQTMADD   G   + R LLPMGS+YEGA+FVLFEMVVY++GEVLGESPEA+R+RHTNLE
Sbjct:    5 SPASLASRICSEIASIFSKPTQPYAPPLEILVSELSSVAERKARVFLYGVGREGLMLKALCMRLAHLGLSSHQVFDMNAPPIAGSDLLIASAGPGGFSTVDAICSVARSKGGRVLVLTAQPENGSSVKYADVICYVPAQTMADDEESGEAAEWRPLLPMGSLYEGALFVLFEMVVYRIGEVLGESPEAVRSRHTNLE 201          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy TrEMBL
Match: I1LDT1_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 295.049 bits (754), Expect = 2.706e-91
Identity = 150/196 (76.53%), Postives = 166/196 (84.69%), Query Frame = -2

Query: 1421 ASLASQICNDIASIFSKPS-IHPPALDLMVAELASTVSQKGRVFLYGVGREGLMLKALCMRLAHLGLSAHFVFDMTTPPITSKDLLVASAGPGGFSTVDAICSVARSHGGRVLLLTAQPETGSSVRHANVVAYV----AAQTMADDGGGEKSRTLLPMGSVYEGAMFVLFEMVVYKLGEVLGESPEAIRARHTNLE 1993
            AS+A++IC  IASIFSKP+  +PP LDL+V ELAS  SQ GRVFLYGVGREGLMLKALCMRL HLGLSAHFVFDMTTPPI + DLL+ASAGPGGFSTVDA+C+VARS+GGRVLLLTAQPETGS V+HAN VAYV     A   AD     KSR LLPMGSVYE A+FVLFEMVVYKLGE LGESPE++R+RHTNLE
Sbjct:   10 ASMATEICTQIASIFSKPTHPYPPPLDLLVTELASVASQNGRVFLYGVGREGLMLKALCMRLFHLGLSAHFVFDMTTPPIDAADLLIASAGPGGFSTVDALCAVARSNGGRVLLLTAQPETGSCVKHANAVAYVAAQTMADDDADAERDAKSRPLLPMGSVYEAALFVLFEMVVYKLGEALGESPESVRSRHTNLE 205          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy TrEMBL
Match: M5X3N7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021377mg PE=4 SV=1)

HSP 1 Score: 291.582 bits (745), Expect = 5.146e-90
Identity = 148/190 (77.89%), Postives = 163/190 (85.79%), Query Frame = -2

Query: 1421 ICNDIASIFSKPS-IHPPALDLMVAELASTVSQKGRVFLYGVGREGLMLKALCMRLAHLGLSAHFVFDMTTPPITSKDLLVASAGPGGFSTVDAICSVARSHGGRVLLLTAQPETGSSVRHANVVAYVAAQTMADD---GGGEKSRTLLPMGSVYEGAMFVLFEMVVYKLGEVLGE-SPEAIRARHTNLE 1975
            ICN IASIF+KP+  HPP LDL+V EL+S  ++ G VFLYGVGREGLMLKALCMRLAHLGLSAH VFDMTTPPI++ DLL+ASAGPGGFSTV+AICSVARSHG RVLLLTAQPETGS V HA+VV YV AQTMA+D   G   +SR LLPMGS YEGA+FVLFEMVVYKL E LG+ SPEAIRARHTNLE
Sbjct:   17 ICNHIASIFTKPTHPHPPVLDLLVNELSSAAARNGGVFLYGVGREGLMLKALCMRLAHLGLSAHMVFDMTTPPISTTDLLIASAGPGGFSTVEAICSVARSHGARVLLLTAQPETGSCVAHASVVGYVPAQTMANDADAGDVMESRPLLPMGSAYEGALFVLFEMVVYKLAEALGQTSPEAIRARHTNLE 206          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy TrEMBL
Match: V4LTT5_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10015742mg PE=4 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 8.432e-90
Identity = 146/196 (74.49%), Postives = 163/196 (83.16%), Query Frame = -2

Query: 1421 MASLASQICNDIASIFSKP-SIHPPALDLMVAELASTVSQKGRVFLYGVGREGLMLKALCMRLAHLGLSAHFVFDMTTPPITSKDLLVASAGPGGFSTVDAICSVARSHGGRVLLLTAQPETGSSVRHANVVAYVAAQTMADDGG--GEK-SRTLLPMGSVYEGAMFVLFEMVVYKLGEVLGESPEAIRARHTNLE 1996
            M+ L+SQICN I+S+FS P S +PP LDL+V ELA    +  RVFL+GVGREGLMLKA  MRL HLGLS+H V DMTTPPI+S DLL+ASAGPGGFSTVDAICSVARS G +VLLLTAQPETGS V+HA  V Y+ AQTMA DGG   EK  R LLPMGSVYEGA+FVLFEMVVYKLGEVLGESPE+IR+RHTNLE
Sbjct:    9 MSKLSSQICNQISSVFSNPTSPYPPPLDLLVTELAGVSRRNSRVFLHGVGREGLMLKAFAMRLFHLGLSSHLVSDMTTPPISSSDLLIASAGPGGFSTVDAICSVARSSGAKVLLLTAQPETGSCVKHATAVCYIPAQTMASDGGDAAEKGERRLLPMGSVYEGALFVLFEMVVYKLGEVLGESPESIRSRHTNLE 204          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy TrEMBL
Match: A0A059DK06_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02801 PE=4 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 1.114e-89
Identity = 146/203 (71.92%), Postives = 166/203 (81.77%), Query Frame = -2

Query: 1421 NGSMASLASQICNDIASIFSKPSIHPPALDLMVAELASTVSQKGRVFLYGVGREGLMLKALCMRLAHLGLSAHFVFDMTTPPITSKDLLVASAGPGGFSTVDAICSVARSHGGRVLLLTAQPETGSSVRHANVVAYVAAQTMADDGG--------GEKSRTLLPMGSVYEGAMFVLFEMVVYKLGEVLGESPEAIRARHTNLE 2005
            + SM +LASQICN IAS+FS P+ HPPALD++V+EL++  S+ GRVFLYGVGREGLM+KALCMRLAHLGL AH VFD+TTPPI   DLL+ASAGPGGFSTVDAICSVARSHGGRV+LLTAQPETG+  RHA+ VA+V AQTMADD              R LLPMGSV+EGAMFVLFEMVVY+LGEVLGESPEA+     NLE
Sbjct:   10 DTSMPALASQICNHIASVFSMPTAHPPALDVLVSELSAAASRGGRVFLYGVGREGLMMKALCMRLAHLGLQAHSVFDVTTPPIAGGDLLLASAGPGGFSTVDAICSVARSHGGRVVLLTAQPETGTCARHASAVAHVPAQTMADDAARRRRRGGRRGGPRPLLPMGSVFEGAMFVLFEMVVYRLGEVLGESPEAVPISPHNLE 212          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy TrEMBL
Match: D7MRN8_ARALL (Sugar isomerase domain-containing protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_495287 PE=4 SV=1)

HSP 1 Score: 283.878 bits (725), Expect = 4.825e-87
Identity = 140/200 (70.00%), Postives = 165/200 (82.50%), Query Frame = -2

Query: 1421 MASLASQICNDIASIFSKP-SIHPPALDLMVAELASTVSQKG--RVFLYGVGREGLMLKALCMRLAHLGLSAHFVFDMTTPPITSKDLLVASAGPGGFSTVDAICSVARSHGGRVLLLTAQPETGSSVRHANVVAYVAAQTMADDGGGE-----KSRTLLPMGSVYEGAMFVLFEMVVYKLGEVLGESPEAIRARHTNLE 1996
            MA+L+SQICN I+S+F+KP S +PP LDL+V ELA+    +   R+FLYGVGREGLMLKA  MRL HLGLS H VFDMTTPPI+S DLL+ASAGPGGFSTVDAICSVA+S+G +V+L+TA+PE GS V+HA  V YV AQTMA D GG+     + R LLPMGSVYEGA+FVLFEMVVYKLG+VLGES E++RARHTNLE
Sbjct:    9 MANLSSQICNQISSVFTKPTSPYPPPLDLLVTELAAISRNRNNSRIFLYGVGREGLMLKAFAMRLFHLGLSTHLVFDMTTPPISSSDLLIASAGPGGFSTVDAICSVAKSNGAKVILITAEPEIGSCVKHATDVCYVPAQTMASDIGGDAAAEMEERRLLPMGSVYEGALFVLFEMVVYKLGDVLGESSESVRARHTNLE 208          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy TrEMBL
Match: M1B4W8_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400014325 PE=4 SV=1)

HSP 1 Score: 283.493 bits (724), Expect = 6.287e-87
Identity = 138/193 (71.50%), Postives = 163/193 (84.46%), Query Frame = -2

Query: 1421 ASLASQICNDIASIFSKPSIHPPALDLMVAELASTVSQKGRVFLYGVGREGLMLKALCMRLAHLGLSAHFVFDMTTPPITSKDLLVASAGPGGFSTVDAICSVARSHGGRVLLLTAQPETGSSVRHANVVAYVAAQTMADD--GGGEKSRTLLPMGSVYEGAMFVLFEMVVYKLGEVLGESPEAIRARHTNLE 1993
            A LAS+ICN I+S+F+  S +P ALD++V E+A   ++ GRVFLYGVGREGLMLKAL MRL HLGLSAH VFDM TPPI   DLL++SAGPGGFSTVDAI  VA+ +G RVLLLTAQP++GSS+++A+ VAY+ AQTMADD  G GEK R LLPMGSVYEGAMFVLFEMVV+KLGE+L +SPEA+RARHTNLE
Sbjct:   10 ALLASKICNQISSVFTAKSPNPAALDVLVQEIADAANRNGRVFLYGVGREGLMLKALSMRLFHLGLSAHCVFDMNTPPIGPSDLLISSAGPGGFSTVDAISGVAKFNGARVLLLTAQPDSGSSMKYASAVAYIPAQTMADDNAGDGEKERPLLPMGSVYEGAMFVLFEMVVFKLGEILKQSPEAVRARHTNLE 202          

BLAST of g.barbadense_cottongen_reftransV1_0093436 vs. ExPASy TrEMBL
Match: M4EK17_BRARP (Uncharacterized protein OS=Brassica rapa subsp. pekinensis GN=Bra029134 PE=4 SV=1)

HSP 1 Score: 283.108 bits (723), Expect = 6.916e-87
Identity = 143/196 (72.96%), Postives = 162/196 (82.65%), Query Frame = -2

Query: 1421 MASLASQICNDIASIFSKPS-IHPPALDLMVAELASTVSQKGRVFLYGVGREGLMLKALCMRLAHLGLSAHFVFDMTTPPITSKDLLVASAGPGGFSTVDAICSVARSHGGRVLLLTAQPETGSSVRHANVVAYVAAQTMADDGGG--EK-SRTLLPMGSVYEGAMFVLFEMVVYKLGEVLGESPEAIRARHTNLE 1996
            M+ L+SQICN I+S+FS P+  +PP LDL+V EL     +  RVFL+GVGREGLMLKA  MRL HLGLS+H V DMTTPPI+S DLL+ASAGPGGFSTVDAICSVA+S G +V+L+TAQPETGS V+HA  V YV AQTMA DGGG  EK  R LLPMGSVYEGA+FVLFEMVVYKLGEVLGESPE+IR RHTNLE
Sbjct:    1 MSRLSSQICNQISSVFSNPTDPYPPTLDLLVTELTGVSRRGARVFLHGVGREGLMLKAFAMRLFHLGLSSHLVSDMTTPPISSPDLLIASAGPGGFSTVDAICSVAKSAGAKVVLITAQPETGSCVKHATDVCYVPAQTMASDGGGATEKGERPLLPMGSVYEGALFVLFEMVVYKLGEVLGESPESIRCRHTNLE 196