g.barbadense_cottongen_reftransV1_0093369, g.barbadense_cottongen_reftransV1_0093369 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: DAAM1_MOUSE (Disheveled-associated activator of morphogenesis 1 OS=Mus musculus GN=Daam1 PE=1 SV=4)

HSP 1 Score: 124.02 bits (310), Expect = 6.026e-27
Identity = 88/282 (31.21%), Postives = 164/282 (58.16%), Query Frame = 3

Query: 3018 CIGPKSLAQPRKNNLKPYHWLKL-TRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAA-------APNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXX-XXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNK-MTLMHYLCK-VKDHLPFLLSL 3830
             +  K++ QP  N LK ++W KL    + G++W E    +  FK   +D+ +LE  FSA          NS     D   +  +S  K +++ +I+ RRA NC I+L+++K+   ++  ++LT+D+      D LE L+KF P K + +LL+ +  + +++ K ++F  E+ ++   + +L+   FK +F+ +V++++  +  I S +EEV  S  LK++++ +L  GN +NKG  RG+A GF++ SL K+ +T++  +K +TL+HYL   V++  P +L+L
Sbjct:  597 TLKKKNIPQP-TNALKSFNWSKLPENKLDGTVWTEIDDTK-VFKI--LDLEDLERTFSAYQRQQEFFVNNSKQKEADAIDDTLSSKLKVKELSVIDGRRAQNCNILLSRLKLSNDEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSGSEEVFRSRALKQLLEVVLAFGNYMNKG-QRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPKVLNL 873          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FH3_ARATH (Formin-like protein 3 OS=Arabidopsis thaliana GN=FH3 PE=2 SV=3)

HSP 1 Score: 117.087 bits (292), Expect = 5.606e-25
Identity = 74/250 (29.60%), Postives = 126/250 (50.40%), Query Frame = 3

Query: 3051 KNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKP--EKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLC 3794
            K  LKP+ W K+    Q  +W E       F     +   +ESLF     N  N +++G+ +  +S R+   + +Q+I+ R+A N  I+L  + +            +   L  + L+ L+K  PT EE   L+ Y+GD   LG  E+F   L+ +P    ++   LF I    +VS L+  L  +    +++RNS    ++++ +L  GN +N GT RG A  F+LD+LLKL++ +  + K TL+H++ 
Sbjct:  328 KTKLKPFFWDKMANPDQKMVWHEISAGSFQF-----NEEAMESLFGY---NDGNKNKNGQKSTDSSLRESPLQYIQIIDTRKAQNLSILLRALNVT--TEEVVDAIKEGNELPVELLQTLLKMAPTSEEELKLRLYSGDLHLLGPAERFLKILVDIPFAFKRIESLLFMISLQEEVSGLKEALGTLEVACKKLRNSRLFLKLLEAVLKTGNRMNVGTFRGDAQAFKLDTLLKLSDVKGTDGKTTLLHFVV 567          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FH8_ORYSJ (Formin-like protein 8 OS=Oryza sativa subsp. japonica GN=FH8 PE=2 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 6.239e-25
Identity = 79/256 (30.86%), Postives = 139/256 (54.30%), Query Frame = 3

Query: 3042 QPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLF----SAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCK 3797
            +PR   LKP HW K+ RA         Q    +F   ++D   +E+LF    + AAP  + G    K+    S R+ E+V  ++ ++A N  I+L  + +   +++ ++L  +   L S+ LE L+K  PTKEE   L+ Y+GD  KLG  E+F   ++ +P    ++   L++  F ++++ LRN    + +  E++R S    ++++ +L  GN +N GT RG A  F+LD+LLKL + +  + K TL+H++ +
Sbjct:  451 EPRPK-LKPLHWDKV-RATSDRAMVWDQLKSSSF---QLDEDMIEALFMNNSTPAAPPREVGR---KAAGVPSFRQEERV--LDPKKAQNIAILLRALNVTREEVSDALLDGNAECLGSELLETLVKMAPTKEEELKLRDYSGDLSKLGSAERFLKAVLDIPFAFKRVDAMLYRANFETEINYLRNSFETLEAACEDLRGSRLFLKLLEAVLRTGNRMNVGTNRGEAKAFKLDTLLKLADVKGTDGKTTLLHFVVQ 696          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FH13_ORYSJ (Formin-like protein 13 OS=Oryza sativa subsp. japonica GN=FH13 PE=2 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.200e-24
Identity = 77/243 (31.69%), Postives = 134/243 (55.14%), Query Frame = 3

Query: 3075 WLKLTRAMQG--SLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCK 3797
            W KL RA+ G  ++W +  K  + F+   +D   +ESLF  +      GS D  + RG SG++  +  L++ +R  N  IML  + +   ++  +++  +   L S+  E L K  PTKEE   LKGY+GD  K+   E+F  +++ VP    ++   L++  F ++V+ LR     + +  EE+R+S    +++  +L  GN +N GT RG A  F+LD+LLKL + ++ + + TL+H++ K
Sbjct:  341 WDKL-RAISGRTTVWDQV-KNSDTFR---VDEEAMESLFLNSG-GGGAGSSDPAARRGGSGKQERR--LLDPKRLQNVAIMLKSLNVAADEVIGALVRGNPEDLGSEFYETLAKMAPTKEEELKLKGYSGDLSKIDPAERFLKDVLGVPFAFERVDAMLYRANFDNEVNYLRKSFGTLEAACEELRSSKLFLKLLDAVLKTGNRMNDGTNRGEARAFKLDTLLKLADIKSTDGRTTLLHFVVK 575          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FH9_ORYSI (Formin-like protein 9 OS=Oryza sativa subsp. indica GN=FH9 PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 7.731e-24
Identity = 80/248 (32.26%), Postives = 121/248 (48.79%), Query Frame = 3

Query: 3060 LKPYHWLKLTRAMQGSL-WAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKS-NRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCK 3797
            LKP HW K+  A   S+ W + +     F   E D   ++SLF   A N     +D ++ N+ AS  K     +IE  R  N  I+L  +             +    L   QLE L+K  PTKEE E L  Y+GD   L   E F   L+ +P    ++ V L+K  F  +V+ ++    +I     E+++S    R+++ +L  GN +N GT RG A  F+LD+LLKL + R  + K TL+H++ K
Sbjct:  474 LKPLHWDKVRAAPNRSMVWNDIRSSSFEF---EFDEQMIKSLF---AYNLQGSMKDEEAMNKTASTTK----HVIEHHRLQNTTILLKTLNAN--TSQVCNSVIQGNGLSVQQLEALVKMKPTKEEEEKLLNYDGDINMLDPAENFVKVLLTIPMAFPRMEVMLYKENFDDEVAHIKMSFAMIEGACTELKSSKLFLRLLEAVLKTGNRMNVGTLRGGASAFKLDALLKLADIRGTDGKTTLLHFVVK 709          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: A0A0A0L132_CUCSA (Formin-like protein OS=Cucumis sativus GN=Csa_4G651990 PE=3 SV=1)

HSP 1 Score: 671.774 bits (1732), Expect = 0.000e+0
Identity = 324/475 (68.21%), Postives = 373/475 (78.53%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDY----TDVA----------NMLQEN-LETGCPKSREIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMATQ 1697
            M LFRK F+RKPPDGLLEISERVYVFDCCF+T++ EE EY+VYI  IV +LR+  TDASFMVFNFREGE+ S I +ILS Y MTVMDYPRQYEGCPLLT E+IHHFL+SSE+W SLGQQN++LMH ERGG PVLAFML ALL+YRKQY+GEQKTL+MIYKQAPRELL L S LNPLPSQLRYLQYVSRRNV SEWPP D+ALTLDCII+RCIPNMDGEGGCRPIFRIYGQDPFMAADRT KVLFSTPKKSK V+ YKQ DCELVKI+I CH+ GDVVLECISLD++LERE M+FR+MFNTAFIRSNILMLNRD+IDILW+ KDQFPKDF AEV+FS++D + S ISI+ P IEEK+GL I+ FA+VQEIFS VD+     D A          N+LQE  L +G    R++  +  +  + E   SE  E    SP      K  + S     A+S  +K EP E+QV  Q+  Q
Sbjct:    1 MALFRKFFYRKPPDGLLEISERVYVFDCCFTTEVLEEDEYKVYIGGIVGQLRESLTDASFMVFNFREGEHHSLITNILSVYDMTVMDYPRQYEGCPLLTMEMIHHFLRSSESWLSLGQQNVLLMHCERGGWPVLAFMLAALLIYRKQYAGEQKTLDMIYKQAPRELLQLMSPLNPLPSQLRYLQYVSRRNVGSEWPPLDRALTLDCIIIRCIPNMDGEGGCRPIFRIYGQDPFMAADRTSKVLFSTPKKSKLVRQYKQVDCELVKIDIHCHIQGDVVLECISLDNDLEREEMMFRVMFNTAFIRSNILMLNRDDIDILWHAKDQFPKDFRAEVLFSEMDSSASLISIELPNIEEKDGLPIEAFARVQEIFSNVDWLSPKADAALNVLQKITASNLLQEKLLSSGSLDRRQLLDLSLEKLILE---SETSEENIRSPRLKIQTKSKLSSELSKAASSVISKLEPSELQVALQLPPQ 472          

HSP 2 Score: 410.223 bits (1053), Expect = 3.007e-115
Identity = 214/279 (76.70%), Postives = 238/279 (85.30%), Query Frame = 3

Query: 2991 LNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV-KDHLPFLL 3824
             N KGRG+  +  K+ +QP+++NLKPYHWLKLTRAMQGSLWAE QK +EA KAPE DMSELESLFSAAAPNSD+G   G SNR ASG K +KV LIELRRAYNCEIML+KVKIPLPD+  SVL LDD+ LD DQ++NLIKFCPTKEE ELLKGY GDK+ LGKCEQFF EL+KVPRVESKLRVF FKIQF  Q SDLRN LN INS +EE+R+SVKLKR+MQTIL LGNALN GTARGSA+GFRLDSLLKLT+TRARNNKMTLMHYLCKV  + LP LL
Sbjct:  984 FNAKGRGLGRMNSKNQSQPKRSNLKPYHWLKLTRAMQGSLWAETQKTDEASKAPEFDMSELESLFSAAAPNSDSGG-SGNSNRRASGPKSDKVHLIELRRAYNCEIMLSKVKIPLPDMMCSVLALDDSALDVDQVDNLIKFCPTKEEMELLKGYGGDKDNLGKCEQFFSELMKVPRVESKLRVFSFKIQFRLQASDLRNSLNTINSASEEIRSSVKLKRVMQTILSLGNALNHGTARGSAIGFRLDSLLKLTDTRARNNKMTLMHYLCKVLAEKLPELL 1261          

HSP 3 Score: 248.44 bits (633), Expect = 3.398e-63
Identity = 128/162 (79.01%), Postives = 136/162 (83.95%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKE-AENEKLKVSTPQR 4667
            HY   V  L EK PELL+F KDLV+LEASTKIQLK LAEEMQAISKGLEKVVQEL  SENDGP+ E FC TLK FL  AE+EVRSLA LYS+VGRNADALALYFGEDPARCPFEQVVSTL NFVRMF RAHEENCKQLE EK KAQKE AE EKLK+ T ++
Sbjct: 1247 HYLCKV--LAEKLPELLDFPKDLVSLEASTKIQLKYLAEEMQAISKGLEKVVQELANSENDGPISEIFCRTLKGFLSHAEAEVRSLASLYSNVGRNADALALYFGEDPARCPFEQVVSTLFNFVRMFARAHEENCKQLEYEKKKAQKEAAEREKLKLGTAKK 1406          

HSP 4 Score: 88.9669 bits (219), Expect = 3.341e-14
Identity = 45/71 (63.38%), Postives = 54/71 (76.06%), Query Frame = 3

Query: 1890 VNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDH 2102
             +S+  K EP ELQVA+Q P Q KII+QR PQ S+S+PVS+ +S+ GSP  I RYHS  SALGITALLHDH
Sbjct:  451 ASSVISKLEPSELQVALQLPPQSKIITQRIPQPSLSTPVSFRSSMQGSPRPILRYHSAPSALGITALLHDH 521          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: G7I7N1_MEDTR (Formin-like protein OS=Medicago truncatula GN=MTR_1g013800 PE=3 SV=2)

HSP 1 Score: 667.537 bits (1721), Expect = 0.000e+0
Identity = 337/561 (60.07%), Postives = 413/561 (73.62%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSA-ISIDFPGIEEKNGLSIDDFAKVQEIFSRVDY----TDVANMLQ---------ENLETGCPKSREIASVLHDD-----KVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLSDANSTRTKFEP--------EEIQVVPQM--ATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKT 1913
            M LFRK F++KPPDGLLEI+ERVYVFD CF+TD+ +E EY+VYI  I+ ++R+HF DASFMVFN REGE QS I++IL +Y MTVMDYPRQYEGCPLLT E+IHHFL+S ENW  LG QNI+LMH ERGG PVLAFML ALL+YRK Y+GEQKTL+MIYKQAPRELL L S LNPLPSQLRYLQY+SRRNV SEWPP D+ALTLDC+I+R IPNMDGEGGCRPIFRIYGQDPF+ A+R+PKVLFSTPK+SK V++YKQADCELVKI+I CHV GDVVLECISLDS++ERE M+FR+MFNTAFIRSNILMLNRDEIDILWNTKD FPK+F  EV+FSD++ + S+ IS+D P IEEK GL ++ FAKV+EIFS V++    TDVAN+LQ         E L++G   S   +++L++      K + KS S+     T SP S   E++S+   QLS+        +P        E+I+    +  A++  PS V S DS ST+ ++ES E    P+++ L N         E   S+ Q  QS     P +D  S +KKT
Sbjct:    1 MALFRKFFYKKPPDGLLEITERVYVFDYCFTTDVIDEDEYKVYIGGIIRQIREHFPDASFMVFNMREGENQSHISNILCDYDMTVMDYPRQYEGCPLLTMEMIHHFLRSGENWLQLGNQNIVLMHCERGGWPVLAFMLAALLIYRKMYTGEQKTLDMIYKQAPRELLQLMSPLNPLPSQLRYLQYISRRNVGSEWPPLDRALTLDCVIIRQIPNMDGEGGCRPIFRIYGQDPFIPANRSPKVLFSTPKRSKLVRHYKQADCELVKIDIHCHVQGDVVLECISLDSDVEREQMMFRVMFNTAFIRSNILMLNRDEIDILWNTKDHFPKNFRVEVLFSDMEASSSSVISVDLPRIEEKEGLPVEAFAKVKEIFSNVEWLDSKTDVANVLQQITASNMLLERLDSGA--SASTSTLLNESLSGRLKFDSKSQSQ-----TNSPKSSVHEERSVSPLQLSEDVPMDKMIKPVKIEELSAEKIKTPASLGPASEPIPSFVLSKDSDSTKKESESSE----PKKS-LENDP-------EFPASTAQVEQSIPLIEPSIDAGSTKKKT 542          

HSP 2 Score: 393.275 bits (1009), Expect = 1.442e-108
Identity = 207/276 (75.00%), Postives = 232/276 (84.06%), Query Frame = 3

Query: 3000 KGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV-KDHLPFLL 3824
            KGRG+     K+  Q +++NLKPYHWLKLTRAM GSLWAE QK +EA +APE DMSELE+LFSA  P+S +  + GKSNR  SG+KP+KVQLIELRRAYNCEIMLTKVKIPLPDL   VL LDD+ L  DQ+ENLIKFCPTKEE + LK Y GDKE LGKCEQFFLEL+KVPRVESKLRVF FK+QF SQVS+L+  LNI+NS +EE+RNSVKLKRIMQTIL LGNALN GTARGSAVGFRLDSLLKLT+TRARNNKMTLMHYLCKV  + LP LL
Sbjct: 1347 KGRGLLRTNAKT--QTKRSNLKPYHWLKLTRAMHGSLWAETQKLDEASRAPEFDMSELETLFSANNPSSSH-EKGGKSNRRGSGQKPDKVQLIELRRAYNCEIMLTKVKIPLPDLMGHVLALDDSVLYVDQVENLIKFCPTKEEMDQLKAYTGDKENLGKCEQFFLELMKVPRVESKLRVFCFKMQFCSQVSELKRDLNIVNSASEEIRNSVKLKRIMQTILSLGNALNHGTARGSAVGFRLDSLLKLTDTRARNNKMTLMHYLCKVLAEKLPELL 1619          

HSP 3 Score: 234.958 bits (598), Expect = 1.009e-58
Identity = 118/158 (74.68%), Postives = 133/158 (84.18%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKE-AENEKLKVS 4655
            HY   V  L EK PELL+F+KDLV LE +TKIQLK LAEEMQAISKGLEKV+QEL+ASENDGPV E FC  LKEFL  AE+EVRSLA LY++VGRNADALA YFGEDP RCPFEQVV+TL+NFVRMF+RAH+EN KQ+E EK KA KE AENEK K++
Sbjct: 1605 HYLCKV--LAEKLPELLDFSKDLVNLEGATKIQLKYLAEEMQAISKGLEKVIQELSASENDGPVSEVFCQILKEFLSDAEAEVRSLAQLYTNVGRNADALASYFGEDPQRCPFEQVVATLMNFVRMFIRAHDENVKQIEYEKKKADKEAAENEKSKLA 1760          

HSP 4 Score: 71.2478 bits (173), Expect = 9.133e-9
Identity = 40/76 (52.63%), Postives = 50/76 (65.79%), Query Frame = 3

Query: 1878 PVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCN-SLPGSPAAISRYHSPLSALGITALLHDH 2102
            P +D  SI+KK +PQ+LQV++Q P Q  IIS R  Q+   +  SY + SL GSP AISRY    SALGIT +L DH
Sbjct:  792 PSIDAKSIKKKIDPQQLQVSLQLPTQSIIISPRIHQAIRPASASYSSTSLLGSPVAISRYQGSPSALGITTVLQDH 867          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: F6H333_VITVI (Formin-like protein OS=Vitis vinifera GN=VIT_04s0008g06530 PE=3 SV=1)

HSP 1 Score: 665.996 bits (1717), Expect = 0.000e+0
Identity = 344/564 (60.99%), Postives = 413/564 (73.23%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLETGCPKSREIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVS 2009
            M LFRK F+RKPPDGLLEISERVYVFDCCF+TD+ E++EY+VY+ +IV +LR+HF DASFMVFNFREG+ QSQI+SILSEY MTVMDYPR YEGCPLLT E+IHHFL+SSE+W SLGQQN++LMH ER G P+LAFML ALL+YRKQY+GEQKTL+MIYKQAPRELL L S LNPLPSQLRYLQYVSRRNV SEWPP D+ALTLDC+ILR IPN+DGEGGCRPIFRIYGQDPFM ADRTPKVLFSTPK+SK V++YKQ DCELVKI+I CH+ GDVVLECISL+ ++ERE M+FR+MFNTAFIRSNILMLNRDEIDILWN+KDQFPKDF AEV+FS++D   S I+ID  G+EEK+GL ++ FAKVQEIFS VD+ D             PK+    +VL              + +T S     LE  S    Q  +      +  PE+++  P+                              P+ AE   SS  ++ + +  ++S +P+         +D N IR+K +PQELQVA+QRPAQ KIISQR PQ+ IS+PVS
Sbjct:    1 MALFRKFFYRKPPDGLLEISERVYVFDCCFTTDVLEDEEYKVYMGSIVGQLREHFPDASFMVFNFREGDSQSQISSILSEYDMTVMDYPRHYEGCPLLTMEMIHHFLRSSESWLSLGQQNVLLMHCERSGWPILAFMLAALLIYRKQYTGEQKTLDMIYKQAPRELLQLMSPLNPLPSQLRYLQYVSRRNVGSEWPPLDRALTLDCVILRIIPNLDGEGGCRPIFRIYGQDPFMVADRTPKVLFSTPKRSKTVRHYKQEDCELVKIDIHCHIQGDVVLECISLEEDMEREEMMFRVMFNTAFIRSNILMLNRDEIDILWNSKDQFPKDFRAEVLFSEMDSGNSLITIDLEGVEEKDGLPMEAFAKVQEIFSNVDWLD-------------PKTDVAFNVL--------------QQITASNVLQELETDSA---QSGETVGLLQELSPEKVEDKPK------------------------------PKAAENNISSTTSMALGKQHMTSAKPS---------VDANLIRRKIDPQELQVALQRPAQSKIISQRIPQTPISNPVS 495          

HSP 2 Score: 257.684 bits (657), Expect = 5.314e-66
Identity = 130/155 (83.87%), Postives = 138/155 (89.03%), Query Frame = 3

Query: 4206 VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVSTPQRS 4670
            VL EK PELL+F KDL+ LEASTKIQLK LAEEMQAISKGLEKVVQELTASENDGPV E FC TLKEFL FAE+EVRSLA LYS VGRNADALALYFGEDPARCPFEQVVSTLLNFVRMF RAHEENCKQLE E+ KAQKEAE+EK+K++  Q S
Sbjct: 1231 VLAEKLPELLDFPKDLLHLEASTKIQLKYLAEEMQAISKGLEKVVQELTASENDGPVSENFCKTLKEFLVFAEAEVRSLASLYSGVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFTRAHEENCKQLEFERKKAQKEAESEKIKINHKQES 1385          

HSP 3 Score: 245.358 bits (625), Expect = 3.890e-62
Identity = 120/148 (81.08%), Postives = 132/148 (89.19%), Query Frame = 3

Query: 3390 QLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV-KDHLPFLLSL 3830
            Q++NLIKFCPTKEE ELLKGYNGDK  LGKCEQFFLEL+KVPRVESKLRVF FKIQF  QVSDL+N LN++NS +EE+RNSVKLKRIMQTIL LGNALN GTARGSA+GFRLDSLLKLT+TRARNNKMTLM+YLCKV  + LP LL  
Sbjct: 1095 QVDNLIKFCPTKEEIELLKGYNGDKGNLGKCEQFFLELMKVPRVESKLRVFSFKIQFRIQVSDLKNNLNVVNSASEEIRNSVKLKRIMQTILSLGNALNHGTARGSAIGFRLDSLLKLTDTRARNNKMTLMNYLCKVLAEKLPELLDF 1242          

HSP 4 Score: 187.193 bits (474), Expect = 2.804e-44
Identity = 90/113 (79.65%), Postives = 97/113 (85.84%), Query Frame = 3

Query: 3000 KGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLP 3338
            KGRG+S  GPK  AQP+K +LKPYHWLKLTRAMQGSLWAE Q+PEEA KAPE DMSELESLFS A PNS+NG   GKSNR ASG K EKVQLI+LRRAYNCEIMLTKVK+PLP
Sbjct:  597 KGRGLSRAGPKIQAQPKKASLKPYHWLKLTRAMQGSLWAETQRPEEASKAPEFDMSELESLFSTAVPNSENGGVGGKSNRRASGPKSEKVQLIDLRRAYNCEIMLTKVKMPLP 709          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: B9RNW2_RICCO (Formin-like protein OS=Ricinus communis GN=RCOM_0920950 PE=3 SV=1)

HSP 1 Score: 655.596 bits (1690), Expect = 0.000e+0
Identity = 346/621 (55.72%), Postives = 416/621 (66.99%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDY----TDVA-------NMLQENLETGCPKSREIASVLHD---DKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQL-----SDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESED 2123
            M LFRK F+RKPPDGLLEISERVYVFDCCF+T+I ++ EY++YI  IV +LRD F D+SFMVFNFREGE+QS I SILSEY MTVMDYPR YEGCPLLT E+IHHFL+SSE+W SL +QN++LMH ERGG PVLAFML +LL+YRKQ+SGEQKTL+MIYKQAPRELL L S LNPLPSQLRYLQYVSRRNV S+WPP D+ALTLDC+ILR IPNMDGEGGCRPIFRIYGQDP MAADRTPKVLFSTPK+SK V++YKQA  +L+K++I CHV GDVV+ECI ++S+LERE M+FR+MFNTAFIRSNILMLN +EID+LWN KDQFPKDF AEV+FS++    S  ++D  G+EEK GL  D FAKVQE FS VD     +D+        + LQENLE    +  EI  V+ +   +KV+ KSN                +K+S  +F L     SDA+STR K E +E+ +    A+Q  PS  PS D +  + + ES                      QE+ VS Q                                R AQ KIIS R  ++  SSP                 +S  SA+GI+ALLHD  AS  E+
Sbjct:    1 MALFRKFFYRKPPDGLLEISERVYVFDCCFTTEILDDDEYKIYIGGIVSQLRDQFPDSSFMVFNFREGEHQSLIGSILSEYDMTVMDYPRHYEGCPLLTMEMIHHFLRSSESWLSLLKQNVLLMHCERGGWPVLAFMLASLLIYRKQFSGEQKTLDMIYKQAPRELLQLMSPLNPLPSQLRYLQYVSRRNVGSQWPPLDRALTLDCVILRVIPNMDGEGGCRPIFRIYGQDPLMAADRTPKVLFSTPKRSKLVRHYKQAVSDLIKLDIHCHVQGDVVMECIHVESDLEREQMIFRVMFNTAFIRSNILMLNSEEIDMLWNVKDQFPKDFRAEVLFSEMHSTSSLATVDLLGLEEKGGLPEDAFAKVQEFFSNVDLLEPESDLTLNLLQQFSRLQENLEIDSSQRVEIDRVMPESTIEKVQGKSN----------------QKESKYNFILSIKSPSDASSTREKSESQELMI----ASQSMPSPKPSPDVNIIQKRVESS---------------------QELHVSLQ--------------------------------RSAQSKIISPRVQKTLRSSP-----------------YSTSSAIGISALLHDDGASNGEE 531          

HSP 2 Score: 415.616 bits (1067), Expect = 5.889e-118
Identity = 225/314 (71.66%), Postives = 253/314 (80.57%), Query Frame = 3

Query: 2889 CAKGLSKAGDTSPQLHSVSXXXXXXXXXXXXXXXL-NLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV-KDHLPFLL 3824
            CAKGLSK G TS   H+            P +  L + KGRG+S   P++ AQPRK NLKPYHWLK+TRAMQGSLWAEAQK +EA K PE DMSELESLFSAAAPN+ +GS   KS+R   G K +KVQLIELRRAYNCEIML+KVKIPL DL S++L +DD+ LD DQ++NLIKFCPTKEE ELLKGY+GDK+ LGKCEQFFLEL+KVPRVESKLRVF FK+QF  QVSDLR  LN++NS AEEVR+S KLKRIMQTIL LGNALN GTARGSAVGFRLDSLLKLT+TRA NNKMTLMHYLCKV  + LP LL
Sbjct:  793 CAKGLSKPGGTSSPSHNGVVNGNVPPVPGPPSGALFSAKGRGLSRSSPRNQAQPRKANLKPYHWLKITRAMQGSLWAEAQKSDEASKTPEFDMSELESLFSAAAPNTGHGSTGEKSSRRTLGSKSDKVQLIELRRAYNCEIMLSKVKIPLSDLMSAILAMDDSVLDVDQVDNLIKFCPTKEEMELLKGYSGDKDNLGKCEQFFLELMKVPRVESKLRVFSFKLQFHPQVSDLRRNLNVVNSAAEEVRSSRKLKRIMQTILSLGNALNHGTARGSAVGFRLDSLLKLTDTRAINNKMTLMHYLCKVLAEKLPELL 1106          

HSP 3 Score: 245.743 bits (626), Expect = 1.672e-62
Identity = 123/161 (76.40%), Postives = 139/161 (86.34%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVSTPQR 4667
            HY   V  L EK PELL+F KDL++LEA+TKIQLK LAEEMQAISKGLEKV+QELTASENDG V E FC +LK FL  AESEVRSLA LYS+VGRNADALALYFGEDPARCPFEQVVSTLLNFV+MF+RAH+ENCKQLEL + +A+KEAENEKLK+   ++
Sbjct: 1092 HYLCKV--LAEKLPELLDFQKDLMSLEAATKIQLKYLAEEMQAISKGLEKVLQELTASENDGHVSEYFCKSLKVFLSSAESEVRSLASLYSTVGRNADALALYFGEDPARCPFEQVVSTLLNFVKMFIRAHDENCKQLELVRKRAEKEAENEKLKMDASKK 1250          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: A0A067FW70_CITSI (Formin-like protein OS=Citrus sinensis GN=CISIN_1g000624mg PE=3 SV=1)

HSP 1 Score: 651.358 bits (1679), Expect = 0.000e+0
Identity = 340/542 (62.73%), Postives = 403/542 (74.35%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDYTD--------------VANMLQENLETGCPKSRE---IASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLS-DANSTRTKFEPEEIQVVPQMATQHTPSAV----PSLDSSSTEGKTESQELQVAPQEAELTN-SSRRNVEIQEI--QVSSQQPAQSTS 1868
            M LFRK F+RKPPDGLLEISERV+VFDCCF+TDI EE+EY+ Y+  IV +LR++F +ASFMVFNFREGE+QSQI  +LSEY MTVMDYPR YEGCPLLT E +HHFL+SSE+W SLG QN++LMH ERGG PVLAFML ALL+YRKQ++GEQKTL+MIYKQAPRELL L S LNPLPSQLRYLQYVSRRNV SEWPP D+ALTLDC+ILR IPN DGEGGC PIFRIYGQDP M ADRTPKVLFSTPK+SK V++YKQADCELVKI+I CH+ GDVVLECISLDS+ ERE M+FR+MFNTAFIRSNILMLNRDEIDILWN+KD F K+F AEV+FS++D A S +S+D PGIEEK+GL I+ FAKVQEIFS VD+ D               +N +QENLET     +    I S L  DK  E+   +AP+++     S +  K  + S + + DANS + K EP+E+ V  Q   Q  P  +    P   SS+++G   S+    AP    +T      +  IQEI  QV   QPA  TS
Sbjct:    1 MALFRKFFYRKPPDGLLEISERVFVFDCCFTTDILEEEEYKEYLGGIVGQLREYFPEASFMVFNFREGEHQSQIGQVLSEYDMTVMDYPRHYEGCPLLTMETVHHFLRSSESWLSLGHQNVLLMHCERGGWPVLAFMLAALLIYRKQFTGEQKTLDMIYKQAPRELLQLMSPLNPLPSQLRYLQYVSRRNVGSEWPPLDRALTLDCVILRVIPNFDGEGGCCPIFRIYGQDPLMVADRTPKVLFSTPKRSKLVRHYKQADCELVKIDIHCHIQGDVVLECISLDSDQEREEMMFRVMFNTAFIRSNILMLNRDEIDILWNSKDLFSKEFRAEVLFSEMDAATSLVSVDLPGIEEKDGLPIEAFAKVQEIFSNVDWLDPKLDVAVNMLQHFTPSNFIQENLETALNAEKGSIMIESALEKDK--EQLKLKAPDNI-GGLASISQGKPFMPSVKPALDANSFKKKNEPKELLVSLQQPAQ--PKIISPRLPQTSSSASQGSPISR-YHSAPSSLGITALLHDHDKYIQEITQQVKRSQPAVPTS 536          

HSP 2 Score: 428.713 bits (1101), Expect = 1.612e-121
Identity = 249/317 (78.55%), Postives = 268/317 (84.54%), Query Frame = 3

Query: 2892 AKGLS--KAGDTSPQLHS-VSXXXXXXXXXXXXXXXLNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV-KDHLPFLLSL 3830
            AKGLS  KA D +P  HS VSNGNIP IPGPPS AP + K RG++   P+  +QPRKNNLKPYHWLKLTRAMQGSLWAEAQK +EA KAPE DMSELESLFSAAAPNSD G + GKSNR  SG KPE+VQLIELRRA NCEIMLTKVKIPLPDL  SVL LDD+ LD DQ++NLIKFCPTKEE E+LK YNGDK  LGKCEQFFLEL+KVPRVESKLRVF FKIQF +QVSDLR  LNIINS +EEVRNS+KLKRIMQTIL LGNALN GTARGSAVGFRLDSLLKLT+TRARNNKMTLMHYLCKV  + LP LL  
Sbjct:  914 AKGLSLSKANDVTPPSHSGVSNGNIPPIPGPPSGAPFSAKMRGLAHASPRLQSQPRKNNLKPYHWLKLTRAMQGSLWAEAQKSDEASKAPEFDMSELESLFSAAAPNSDLGGKSGKSNR-RSGPKPERVQLIELRRANNCEIMLTKVKIPLPDLMGSVLALDDSALDIDQVDNLIKFCPTKEEMEVLKNYNGDKGNLGKCEQFFLELMKVPRVESKLRVFSFKIQFQTQVSDLRTSLNIINSASEEVRNSIKLKRIMQTILSLGNALNHGTARGSAVGFRLDSLLKLTDTRARNNKMTLMHYLCKVLAEKLPELLGF 1229          

HSP 3 Score: 244.973 bits (624), Expect = 3.667e-62
Identity = 126/160 (78.75%), Postives = 132/160 (82.50%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVSTPQ 4664
            HY   V  L EK PELL F KDLV+LEASTKIQLK LAEEMQAISKGLEKVVQELTASENDG V   FC  LKEFL +AE EVRSLALLYSSVGRNADALA YFGEDPARCPFEQVVSTLLNFV+MFV AHEENC+QLE E+ KA+K  ENEKLK    Q
Sbjct: 1213 HYLCKV--LAEKLPELLGFPKDLVSLEASTKIQLKFLAEEMQAISKGLEKVVQELTASENDGEVSGNFCKLLKEFLSYAEGEVRSLALLYSSVGRNADALAQYFGEDPARCPFEQVVSTLLNFVKMFVLAHEENCRQLEFERKKAEKAVENEKLKTQKGQ 1370          

HSP 4 Score: 85.5001 bits (210), Expect = 3.739e-13
Identity = 47/76 (61.84%), Postives = 55/76 (72.37%), Query Frame = 3

Query: 1878 PVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHD 2105
            P LD NS +KK EP+EL V++Q+PAQPKIIS R PQ+S S+         GSP  ISRYHS  S+LGITALLHDHD
Sbjct:  450 PALDANSFKKKNEPKELLVSLQQPAQPKIISPRLPQTSSSAS-------QGSP--ISRYHSAPSSLGITALLHDHD 516          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: A0A067FJA7_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g000624mg PE=4 SV=1)

HSP 1 Score: 650.588 bits (1677), Expect = 0.000e+0
Identity = 340/542 (62.73%), Postives = 403/542 (74.35%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDYTD--------------VANMLQENLETGCPKSRE---IASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLS-DANSTRTKFEPEEIQVVPQMATQHTPSAV----PSLDSSSTEGKTESQELQVAPQEAELTN-SSRRNVEIQEI--QVSSQQPAQSTS 1868
            M LFRK F+RKPPDGLLEISERV+VFDCCF+TDI EE+EY+ Y+  IV +LR++F +ASFMVFNFREGE+QSQI  +LSEY MTVMDYPR YEGCPLLT E +HHFL+SSE+W SLG QN++LMH ERGG PVLAFML ALL+YRKQ++GEQKTL+MIYKQAPRELL L S LNPLPSQLRYLQYVSRRNV SEWPP D+ALTLDC+ILR IPN DGEGGC PIFRIYGQDP M ADRTPKVLFSTPK+SK V++YKQADCELVKI+I CH+ GDVVLECISLDS+ ERE M+FR+MFNTAFIRSNILMLNRDEIDILWN+KD F K+F AEV+FS++D A S +S+D PGIEEK+GL I+ FAKVQEIFS VD+ D               +N +QENLET     +    I S L  DK  E+   +AP+++     S +  K  + S + + DANS + K EP+E+ V  Q   Q  P  +    P   SS+++G   S+    AP    +T      +  IQEI  QV   QPA  TS
Sbjct:    1 MALFRKFFYRKPPDGLLEISERVFVFDCCFTTDILEEEEYKEYLGGIVGQLREYFPEASFMVFNFREGEHQSQIGQVLSEYDMTVMDYPRHYEGCPLLTMETVHHFLRSSESWLSLGHQNVLLMHCERGGWPVLAFMLAALLIYRKQFTGEQKTLDMIYKQAPRELLQLMSPLNPLPSQLRYLQYVSRRNVGSEWPPLDRALTLDCVILRVIPNFDGEGGCCPIFRIYGQDPLMVADRTPKVLFSTPKRSKLVRHYKQADCELVKIDIHCHIQGDVVLECISLDSDQEREEMMFRVMFNTAFIRSNILMLNRDEIDILWNSKDLFSKEFRAEVLFSEMDAATSLVSVDLPGIEEKDGLPIEAFAKVQEIFSNVDWLDPKLDVAVNMLQHFTPSNFIQENLETALNAEKGSIMIESALEKDK--EQLKLKAPDNI-GGLASISQGKPFMPSVKPALDANSFKKKNEPKELLVSLQQPAQ--PKIISPRLPQTSSSASQGSPISR-YHSAPSSLGITALLHDHDKYIQEITQQVKRSQPAVPTS 536          

HSP 2 Score: 312.768 bits (800), Expect = 4.152e-84
Identity = 189/247 (76.52%), Postives = 205/247 (83.00%), Query Frame = 3

Query: 2892 AKGLS--KAGDTSPQLHS-VSXXXXXXXXXXXXXXXLNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEV 3623
            AKGLS  KA D +P  HS VSNGNIP IPGPPS AP + K RG++   P+  +QPRKNNLKPYHWLKLTRAMQGSLWAEAQK +EA KAPE DMSELESLFSAAAPNSD G + GKSNR  SG KPE+VQLIELRRA NCEIMLTKVKIPLPDL  SVL LDD+ LD DQ++NLIKFCPTKEE E+LK YNGDK  LGKCEQFFLEL+KVPRVESKLRVF FKIQF +QVSDLR  LNIINS +EE 
Sbjct:  914 AKGLSLSKANDVTPPSHSGVSNGNIPPIPGPPSGAPFSAKMRGLAHASPRLQSQPRKNNLKPYHWLKLTRAMQGSLWAEAQKSDEASKAPEFDMSELESLFSAAAPNSDLGGKSGKSNR-RSGPKPERVQLIELRRANNCEIMLTKVKIPLPDLMGSVLALDDSALDIDQVDNLIKFCPTKEEMEVLKNYNGDKGNLGKCEQFFLELMKVPRVESKLRVFSFKIQFQTQVSDLRTSLNIINSASEEA 1159          

HSP 3 Score: 84.7297 bits (208), Expect = 5.181e-13
Identity = 47/76 (61.84%), Postives = 55/76 (72.37%), Query Frame = 3

Query: 1878 PVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHD 2105
            P LD NS +KK EP+EL V++Q+PAQPKIIS R PQ+S S+         GSP  ISRYHS  S+LGITALLHDHD
Sbjct:  450 PALDANSFKKKNEPKELLVSLQQPAQPKIISPRLPQTSSSASQ-------GSP--ISRYHSAPSSLGITALLHDHD 516          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: V4UMC3_9ROSI (Formin-like protein OS=Citrus clementina GN=CICLE_v10007257mg PE=3 SV=1)

HSP 1 Score: 650.203 bits (1676), Expect = 0.000e+0
Identity = 339/542 (62.55%), Postives = 402/542 (74.17%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDYTD--------------VANMLQENLETGCPKSRE---IASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLS-DANSTRTKFEPEEIQVVPQMATQHTPSAV----PSLDSSSTEGKTESQELQVAPQEAELTN-SSRRNVEIQEI--QVSSQQPAQSTS 1868
            M LFRK F+RKPPDGLLEISERV+VFDCCF+TDI EE+EY+ Y+  IV +L ++F +ASFMVFNFREGE+QSQI  +LSEY MTVMDYPR YEGCPLLT E +HHFL+SSE+W SLG QN++LMH ERGG PVLAFML ALL+YRKQ++GEQKTL+MIYKQAPRELL L S LNPLPSQLRYLQYVSRRNV SEWPP D+ALTLDC+ILR IPN DGEGGC PIFRIYGQDP M ADRTPKVLFSTPK+SK V++YKQADCELVKI+I CH+ GDVVLECISLDS+ ERE M+FR+MFNTAFIRSNILMLNRDEIDILWN+KD F K+F AEV+FS++D A S +S+D PGIEEK+GL I+ FAKVQEIFS VD+ D               +N +QENLET     +    I S L  DK  E+   +AP+++     S +  K  + S + + DANS + K EP+E+ V  Q   Q  P  +    P   SS+++G   S+    AP    +T      +  IQEI  QV   QPA  TS
Sbjct:    1 MALFRKFFYRKPPDGLLEISERVFVFDCCFTTDILEEEEYKEYLGGIVGQLHEYFPEASFMVFNFREGEHQSQIGQVLSEYDMTVMDYPRHYEGCPLLTMETVHHFLRSSESWLSLGHQNVLLMHCERGGWPVLAFMLAALLIYRKQFTGEQKTLDMIYKQAPRELLQLMSPLNPLPSQLRYLQYVSRRNVGSEWPPLDRALTLDCVILRVIPNFDGEGGCCPIFRIYGQDPLMVADRTPKVLFSTPKRSKLVRHYKQADCELVKIDIHCHIQGDVVLECISLDSDQEREEMMFRVMFNTAFIRSNILMLNRDEIDILWNSKDLFSKEFRAEVLFSEMDAATSLVSVDLPGIEEKDGLPIEAFAKVQEIFSNVDWLDPKLDVAVNMLQHFTPSNFIQENLETALNAEKGSIMIESALEKDK--EQLKLKAPDNI-GGLASISQGKPFMPSVKPALDANSFKKKNEPKEVLVSLQQPAQ--PKIISPRLPQTSSSASQGSPISR-YHSAPSSLGITALLHDHDKYIQEITQQVKRSQPAVPTS 536          

HSP 2 Score: 428.713 bits (1101), Expect = 1.355e-121
Identity = 249/317 (78.55%), Postives = 268/317 (84.54%), Query Frame = 3

Query: 2892 AKGLS--KAGDTSPQLHS-VSXXXXXXXXXXXXXXXLNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV-KDHLPFLLSL 3830
            AKGLS  KA D +P  HS VSNGNIP IPGPPS AP + K RG++   P+  +QPRKNNLKPYHWLKLTRAMQGSLWAEAQK +EA KAPE DMSELESLFSAAAPNSD G + GKSNR  SG KPE+VQLIELRRA NCEIMLTKVKIPLPDL  SVL LDD+ LD DQ++NLIKFCPTKEE E+LK YNGDK  LGKCEQFFLEL+KVPRVESKLRVF FKIQF +QVSDLR  LNIINS +EEVRNS+KLKRIMQTIL LGNALN GTARGSAVGFRLDSLLKLT+TRARNNKMTLMHYLCKV  + LP LL  
Sbjct:  903 AKGLSLSKANDVTPPSHSGVSNGNIPPIPGPPSGAPFSAKMRGLAHASPRLQSQPRKNNLKPYHWLKLTRAMQGSLWAEAQKSDEASKAPEFDMSELESLFSAAAPNSDLGGKSGKSNR-RSGPKPERVQLIELRRANNCEIMLTKVKIPLPDLMGSVLALDDSALDIDQVDNLIKFCPTKEEMEVLKNYNGDKGNLGKCEQFFLELMKVPRVESKLRVFSFKIQFQTQVSDLRTSLNIINSASEEVRNSIKLKRIMQTILSLGNALNHGTARGSAVGFRLDSLLKLTDTRARNNKMTLMHYLCKVLAEKLPELLGF 1218          

HSP 3 Score: 245.358 bits (625), Expect = 3.477e-62
Identity = 126/160 (78.75%), Postives = 132/160 (82.50%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVSTPQ 4664
            HY   V  L EK PELL F KDLV+LEASTKIQLK LAEEMQAISKGLEKVVQELTASENDG V   FC  LKEFL +AE EVRSLALLYSSVGRNADALA YFGEDPARCPFEQVVSTLLNFV+MFV AHEENC+QLE E+ KA+K  ENEKLK    Q
Sbjct: 1202 HYLCKV--LAEKLPELLGFPKDLVSLEASTKIQLKFLAEEMQAISKGLEKVVQELTASENDGEVSGNFCKLLKEFLSYAEGEVRSLALLYSSVGRNADALAQYFGEDPARCPFEQVVSTLLNFVKMFVLAHEENCRQLEFERKKAEKAVENEKLKTQKGQ 1359          

HSP 4 Score: 84.3445 bits (207), Expect = 8.316e-13
Identity = 46/76 (60.53%), Postives = 55/76 (72.37%), Query Frame = 3

Query: 1878 PVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHD 2105
            P LD NS +KK EP+E+ V++Q+PAQPKIIS R PQ+S S+         GSP  ISRYHS  S+LGITALLHDHD
Sbjct:  450 PALDANSFKKKNEPKEVLVSLQQPAQPKIISPRLPQTSSSAS-------QGSP--ISRYHSAPSSLGITALLHDHD 516          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: B9HAW7_POPTR (Formin-like protein OS=Populus trichocarpa GN=POPTR_0006s27960g PE=3 SV=2)

HSP 1 Score: 649.818 bits (1675), Expect = 0.000e+0
Identity = 342/557 (61.40%), Postives = 408/557 (73.25%), Query Frame = 3

Query:  498 MVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDY----TDVANML-------QENLETGCPKSREIASVLHDD---KVEEKSNSEAPEHVTTSPTSDALEKQSICSFQ-LSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESED 2123
            MVFNFREG+ QSQI+S+LSE+ MTVMDYPR YE CP+L+ E+IHHFL+SSE+W SLGQQN++LMH ERGG PVLAFML ALLLY KQ++GEQ+TL+MIYKQ PRELL L S +NPLPSQLRYLQYVSRRNV ++WPP D+ALTLDCIILR +P MD EGGCRPIFRIYGQDPFMAADR PKVLFSTPK+S+FV+++KQA+CELVKI+I CH+ GD+V+ECISLDS+ ERE M+FR+MFNTAFIRSNILM NRDEID LWN KDQFPKDF AEV+FS++D A S  +ID PG+EEK+GL ++ FA+V EIFS VD+    TDVA  +       QENL++  P+  EI SVL +    KV+EK   +  E+ T SPTS    KQSI SF+  SD NS R + EP+E++V    A    PS   S D +S   K E QEL+VA                           Q T +  P  D NS +KK EPQELQVA+Q  AQ K+ISQR PQ S SSPVSY NSL  SP  +SRYH   S LGITALL+DH AS+ E+
Sbjct:    1 MVFNFREGDNQSQISSVLSEFDMTVMDYPRHYESCPILSMEMIHHFLRSSESWLSLGQQNVVLMHCERGGWPVLAFMLAALLLYGKQFTGEQRTLDMIYKQGPRELLQLMSPINPLPSQLRYLQYVSRRNVGTQWPPLDRALTLDCIILRLVPGMDTEGGCRPIFRIYGQDPFMAADRNPKVLFSTPKRSRFVRHFKQAECELVKIDIHCHIQGDIVMECISLDSDHEREQMMFRVMFNTAFIRSNILMFNRDEIDTLWNAKDQFPKDFRAEVLFSEMDSATSIAAIDLPGLEEKDGLPMEAFARVHEIFSNVDWPDTKTDVAQNVHQHIIAPQENLDSS-PQRAEIGSVLMESTLVKVQEKLKLKESENKTPSPTSITPMKQSILSFEPFSDTNSVREEAEPQELKV----ALHSVPSIKSSPDENSAGEKVEHQELKVA--------------------------LQFTPSIKPSSDENSTQKKVEPQELQVALQLSAQSKLISQRVPQRSHSSPVSYSNSLQASPVPMSRYHGAPSNLGITALLYDHAASKGEE 526          

HSP 2 Score: 404.831 bits (1039), Expect = 3.598e-114
Identity = 215/276 (77.90%), Postives = 234/276 (84.78%), Query Frame = 3

Query: 2994 NLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFK-------APEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 3800
            N KGRG+  I  K+  QPRK NLKPYHWLKLTRA+ GSLWAEAQK +EA K       APE DMSELE+LFSAAAPNSD GS  GKSNR   G K +K+QLIELRRAYNCEIML+KVKIPLPDL SSVL LDDT LD DQ++NLIKFCPTKEE ELLKGYNGDKE LGKCE+FFLE++KVPR ESKLRVF FKIQF SQVSDLR  LNI+NS +EE+R+S KLKRIMQTIL LGNALN GTARGSAVGFR+DSLLKLT+TRARNNKMTLMHYLCKV
Sbjct:  869 NAKGRGMFRINSKN--QPRKANLKPYHWLKLTRAVSGSLWAEAQKADEASKYTPFVLLAPEFDMSELETLFSAAAPNSDRGSTAGKSNRRTVGHKSDKIQLIELRRAYNCEIMLSKVKIPLPDLMSSVLALDDTALDVDQVDNLIKFCPTKEEMELLKGYNGDKENLGKCEKFFLEMMKVPRAESKLRVFSFKIQFQSQVSDLRGNLNIVNSASEEIRSSAKLKRIMQTILSLGNALNFGTARGSAVGFRMDSLLKLTDTRARNNKMTLMHYLCKV 1142          

HSP 3 Score: 174.481 bits (441), Expect = 2.171e-40
Identity = 89/117 (76.07%), Postives = 97/117 (82.91%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVV 4535
            HY   V  L EK  ELL+F KDLV+LEA+TKIQLK LAEEMQA+SKGLEKV+QEL+A+ENDG V E FC  LKEFL FAESEVRSLA LYS VGRNADALALYFGEDPARC FEQ +
Sbjct: 1137 HYLCKV--LAEKLAELLDFPKDLVSLEAATKIQLKYLAEEMQAVSKGLEKVMQELSAAENDGLVSEIFCKILKEFLAFAESEVRSLACLYSCVGRNADALALYFGEDPARCTFEQGI 1251          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: A0A067KES4_JATCU (Formin-like protein OS=Jatropha curcas GN=JCGZ_07089 PE=3 SV=1)

HSP 1 Score: 643.654 bits (1659), Expect = 0.000e+0
Identity = 330/581 (56.80%), Postives = 402/581 (69.19%), Query Frame = 3

Query:  423 EEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDYTDVA-----------NMLQENLETGCPKSREIASVLHD---DKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESED 2123
            E+ EY+VYI  IV +LR++F D+SFMVFNFREGE QSQI SILS+Y MTVMDYPR YEGCPLLT E+IHHFL+SSE+W +L QQN++LMH ERGG P+L+FML ALL+YRKQ++GEQKTL+MIYKQAPRELL L S LNPLPSQLRYLQYVSRRNV S+WPP D+ALTLDC+ILR IPNMDGEGGCRPIFRIYGQDP M ADRTPKVLFSTPK+SK V+YYKQ  C L+KI+I CH+ GDVV+ECI+++S+LERE M+FR+MFNTAFIRSNILMLNRDEID LWN KDQFPKDF AEV+FS++D   S  SID PG+EEK GL  + FAKVQE+FS VD+ D               LQENLE   P+   I  +      D++ ++S  +  E    SPT  +   +SI      DANS R K E           +Q +P+A   ++    +   +     + P       S+R  VE QE+ ++SQ  +  +S   P  DV+SIR+K    ELQV++QRP Q  IIS R  ++  SSP                YH  LS LGI+ALLHDH AS  E+
Sbjct:    2 EDDEYKVYIGGIVSQLREYFPDSSFMVFNFREGENQSQIGSILSDYDMTVMDYPRHYEGCPLLTMEMIHHFLRSSESWLTLLQQNVLLMHCERGGWPILSFMLAALLIYRKQFTGEQKTLDMIYKQAPRELLQLMSPLNPLPSQLRYLQYVSRRNVGSQWPPLDRALTLDCVILRIIPNMDGEGGCRPIFRIYGQDPLMVADRTPKVLFSTPKRSKLVRYYKQEVCNLIKIDIHCHIQGDVVMECINVESDLEREQMMFRVMFNTAFIRSNILMLNRDEIDTLWNVKDQFPKDFRAEVLFSEMDSTSSLASIDLPGLEEKGGLPEEAFAKVQEMFSNVDWLDTKPDAALNVLQHFTTLQENLENISPQRAAIGGLTPKSAFDRIIDESKLKESERKCRSPTYLSSRDRSI------DANSMRRKLE-----------SQASPTANSIVEKVEADNNADQSIHSIKPSPD--VYSTREKVEPQELTIASQ--SMPSSKLCP--DVHSIRRKVGAPELQVSLQRPTQSMIISPRVSKTPRSSP----------------YHGALSTLGISALLHDHSASNGEE 543          

HSP 2 Score: 402.905 bits (1034), Expect = 1.102e-113
Identity = 211/276 (76.45%), Postives = 236/276 (85.51%), Query Frame = 3

Query: 3000 KGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV-KDHLPFLL 3824
            +GRG+S   P++   PRK NLKPYHWLKLTRAMQGSLWAE  K ++A KAPE DMSELESLFSA APNS +G  + KSNR   G KPEKVQLIELRRAYNCEIML+KVKIPLPDL  SVL LDD+ LD DQ++NLIKFCPTKEE ELLKGYNGDKE LG+CEQFF+EL+KVPRVESKLRVF FK+QF  QV+DLR  LN++NS AEE+R+S+KLKRIMQTIL LGNALN GTARGSA+GFRLDSLLKLT+TRARNNKMTLMHYLCKV  + LP LL
Sbjct:  831 RGRGLSRANPRNQV-PRKANLKPYHWLKLTRAMQGSLWAETPKSDDASKAPEFDMSELESLFSAVAPNSGHGGGE-KSNRRTRGPKPEKVQLIELRRAYNCEIMLSKVKIPLPDLMGSVLALDDSALDVDQVDNLIKFCPTKEEMELLKGYNGDKENLGRCEQFFMELMKVPRVESKLRVFSFKLQFHPQVTDLRRNLNVVNSAAEEIRSSIKLKRIMQTILSLGNALNHGTARGSAIGFRLDSLLKLTDTRARNNKMTLMHYLCKVLSEKLPELL 1104          

HSP 3 Score: 254.603 bits (649), Expect = 2.617e-65
Identity = 127/161 (78.88%), Postives = 140/161 (86.96%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVSTPQR 4667
            HY   V  L+EK PELL+F KDL+ LEA+TKIQLK LAEEMQAISKGLEKVVQELTASENDG V E+FC  LKEFL  AESEVRSLALLYS+VGRNADALALYFGEDPARCPFEQVVSTLLNFVRMF RAHEENCKQLE E+ KA+++AENEKL++   +R
Sbjct: 1090 HYLCKV--LSEKLPELLDFPKDLMNLEAATKIQLKYLAEEMQAISKGLEKVVQELTASENDGQVSESFCKILKEFLSSAESEVRSLALLYSTVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFSRAHEENCKQLEFERKKAERDAENEKLRMGASKR 1248          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: M5Y4B1_PRUPE (Formin-like protein OS=Prunus persica GN=PRUPE_ppa000320mg PE=3 SV=1)

HSP 1 Score: 638.647 bits (1646), Expect = 0.000e+0
Identity = 336/560 (60.00%), Postives = 391/560 (69.82%), Query Frame = 3

Query:  498 MVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDY----TDVA-NMLQENLETGCPKSREIASV---------LHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMAT------QHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASES 2117
            MVFNFR+GE QS I +ILSEY MTVMDYPR YEGCPLLT E IHHFL+SSE+W SLGQQN++LMH ERGG  VLAFML ALL+YRKQY+GE KTL++IYKQAPRELL L S LNP+PSQLRYLQY++RRNV SEWPP D+ALTLDCII+R IPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPK+SK V++YKQADCELVKI+I CH+ GDVVLECISLD++LERE M+FRIMFNTAFIRSNILMLNR++IDILWN KDQFPKDF AEV+FS++D A S ISID PG+EEK+GL ++ FAKVQEIFS VD+    TDV  N+LQ    +G  ++ + ASV         L       KS  +A E+    PTS A  KQ   S   ++  +   +  P ++   P+ A       Q+ P    SL  SS E    +             NS R+ +E QE+QV                                A+ RPAQ KIISQR PQ+S S+PVSYCNSL GSP  ISRYHS  SALGITALL DH AS S
Sbjct:    1 MVFNFRDGENQSLICNILSEYDMTVMDYPRHYEGCPLLTMEAIHHFLRSSESWLSLGQQNVLLMHCERGGWLVLAFMLAALLIYRKQYTGEHKTLDIIYKQAPRELLQLMSPLNPMPSQLRYLQYITRRNVGSEWPPLDRALTLDCIIIRFIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKRSKLVRHYKQADCELVKIDIHCHIQGDVVLECISLDNDLEREEMMFRIMFNTAFIRSNILMLNREDIDILWNVKDQFPKDFRAEVLFSEMDAATSLISIDLPGMEEKDGLPVEAFAKVQEIFSNVDWLDPKTDVTLNVLQRIASSGALQNLDSASVHNTETGSLLLESTSANLKSEVKASENNIKGPTSVAQGKQDSASAYTAETGNLLLESLPAKLISEPKAAENSIKSPQYRPHGKQSL--SSFEPSLNA-------------NSIRKKIEPQELQV--------------------------------ALLRPAQSKIISQRAPQASRSAPVSYCNSLQGSPVPISRYHSAPSALGITALLQDHAASNS 513          

HSP 2 Score: 412.92 bits (1060), Expect = 6.964e-117
Identity = 215/279 (77.06%), Postives = 241/279 (86.38%), Query Frame = 3

Query: 2991 LNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV-KDHLPFLL 3824
             +LKGRG++  G ++ AQP+K NLKPYHWLKLTRAMQGSLWAEAQK +EA K PE DMSELESLFSA  PNSD+G+  GKS+R  +G K EKVQLIELRRAYNCEIML+KVKIPLPD+ SSVL LDD+ LD DQ+ENLIKFCPTKEE ELLKGY+GDKE LG+CEQFFLEL+KVPRVE KLRVF FKIQF SQVSDLR  LN +NS A+++R+S KLKRIMQTIL LGNALN GTARGSA+GFRLDSLLKLT+TRARNNKMTLMHYLCKV  + LP LL
Sbjct:  850 FSLKGRGLTRPGSRNQAQPKKANLKPYHWLKLTRAMQGSLWAEAQKADEATKPPEFDMSELESLFSAVTPNSDHGNTGGKSSRRTTGPKSEKVQLIELRRAYNCEIMLSKVKIPLPDVMSSVLALDDSALDIDQVENLIKFCPTKEEMELLKGYSGDKENLGRCEQFFLELMKVPRVEPKLRVFSFKIQFHSQVSDLRKNLNTVNSVADQIRSSEKLKRIMQTILSLGNALNHGTARGSAIGFRLDSLLKLTDTRARNNKMTLMHYLCKVLAEKLPELL 1128          

HSP 3 Score: 262.692 bits (670), Expect = 8.675e-68
Identity = 134/164 (81.71%), Postives = 142/164 (86.59%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVST-PQRSL 4673
            HY   V  L EK PELL+F KDL +LEASTKIQLK LAEEMQAISKGLEKVVQELTASENDGPV ETFC TLKEFL  AE+EVRSLA LYS+VGRNADALALYFGEDPARCPFEQVVSTLLNFVRMF RAHEENCKQLE EK KAQKEA+NEK+K  T P++ L
Sbjct: 1114 HYLCKV--LAEKLPELLDFPKDLTSLEASTKIQLKYLAEEMQAISKGLEKVVQELTASENDGPVSETFCKTLKEFLSHAEAEVRSLASLYSNVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFARAHEENCKQLEFEKKKAQKEAQNEKMKAGTVPKKEL 1275