g.barbadense_cottongen_reftransV1_0093369, g.barbadense_cottongen_reftransV1_0093369 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FH16_ARATH (Formin-like protein 16 OS=Arabidopsis thaliana GN=FH16 PE=3 SV=2)

HSP 1 Score: 251.136 bits (640), Expect = 4.718e-68
Identity = 143/252 (56.75%), Postives = 184/252 (73.02%), Query Frame = 3

Query: 3048 RKNNLKPYHWLKLTRAMQGSLWAEAQ-KPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 3800
            ++++LKP HW+K+TRA+QGSLW E Q +  E+  A E+D+ E+E+LFS  A             +     KPEKV LI+L+RA N  + L  +K+PLPD+ ++V+ +D++ LD DQ+ENLI+ CPTKEE ELLK Y GDK  LGK EQ  LEL+KVPR E+KLRV  FKI F ++++  R  LN++NS  EEVR+S  LK IM+ IL+LGN LN+GTARGSAVGFRLDSLL L+ETRA NNKMTLMHYLCKV
Sbjct:  188 KRSSLKPLHWVKITRALQGSLWDELQIQYGESQTAIELDVPEIETLFSVGA-------------KPRPKPKPEKVPLIDLKRANNTIVNLKILKMPLPDMMAAVMAMDESVLDVDQIENLIQLCPTKEEMELLKNYTGDKATLGKSEQCLLELMKVPRFEAKLRVLSFKIPFGTKITKFRKMLNVVNSACEEVRSSQMLKEIMKIILFLGNTLNQGTARGSAVGFRLDSLLILSETRADNNKMTLMHYLCKV 426          

HSP 2 Score: 161.77 bits (408), Expect = 3.198e-39
Identity = 83/148 (56.08%), Postives = 109/148 (73.65%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKE 4628
            HY   V  L  K+ +LL+F KDL +LE++ +I LK LAEE+ AI+KGLEK+ QELTASE DGPV + F   LK+F+  AE++V +++ LYSS   NADALA YFGEDP   PFE+V +TLL+F+R+F +AH+EN KQ +LEK KA  E
Sbjct:  421 HYLCKV--LASKAADLLDFHKDLQSLESTLEINLKSLAEEIHAITKGLEKLKQELTASETDGPVSQVFRKLLKDFISSAETQVATVSTLYSSARINADALAHYFGEDPNHYPFEKVSATLLSFIRLFKKAHQENVKQEDLEKKKAATE 566          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FH15B_ARATH (Putative formin-like protein 15b OS=Arabidopsis thaliana GN=FH15B PE=3 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 1.231e-61
Identity = 119/188 (63.30%), Postives = 146/188 (77.66%), Query Frame = 3

Query: 3237 RGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 3800
            + A   K + ++ I+LRRA + EIMLTKV IPL D+ ++VL +D+  LD DQ+ENLI+FCPTKEE ELLK Y GDK  LGKCEQ+FLE++KVP VESKLR F FKIQF +Q+++L  GLN +NS  EEVR S KLK IM  IL +GN LN+GTA GSAVGF+L SLL L++T A N+KMTLMHYLCKV
Sbjct:   19 KQADLEKKKAMKQIDLRRANDTEIMLTKVNIPLADMMAAVLGMDEYVLDVDQIENLIRFCPTKEEMELLKNYTGDKATLGKCEQYFLEVMKVPGVESKLRAFSFKIQFGTQIAELNKGLNAVNSACEEVRTSEKLKEIMANILCMGNILNQGTAEGSAVGFKLKSLLILSDTCAPNSKMTLMHYLCKV 206          

HSP 2 Score: 176.022 bits (445), Expect = 8.363e-46
Identity = 90/155 (58.06%), Postives = 120/155 (77.42%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLK 4649
            HY   V  L  K+ +LL+F KDL +LE+++KIQLK LAEE+QAI+KGLEK+ ++LTASE+DGPV + F   LK+F+  AE++V +++ LYSS G+NADALA YFGEDP   PFE+V +TLL+F+R+F +AHEEN KQ +L+K K  KEAE EK K
Sbjct:  201 HYLCKV--LASKASDLLDFHKDLESLESASKIQLKSLAEEIQAITKGLEKLNKQLTASESDGPVSQVFRKVLKDFISMAETQVATVSSLYSS-GKNADALAHYFGEDPNHYPFEKVTTTLLSFIRLFKKAHEENVKQADLDKNKDAKEAEMEKTK 352          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FH21B_ARATH (Formin-like protein 21b OS=Arabidopsis thaliana GN=FH21B PE=3 SV=1)

HSP 1 Score: 222.246 bits (565), Expect = 3.949e-61
Identity = 116/176 (65.91%), Postives = 141/176 (80.11%), Query Frame = 3

Query: 3273 LIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 3800
            LI+ RRA+N  IML KV++PLPD+ ++VL +D++ LD DQ+ENLI+FCPTKEE +LLK Y GDK  LGKCEQ+FLEL+KVP VESKLRVF FKI F +Q+ +L  GLN +NS  EE+R S KLK IM+ IL LGN LN+GTARGSAVGF+LDSLL L+E  + N  MTLMHYLCKV
Sbjct:   59 LIDQRRAFNTMIMLQKVEMPLPDMMAAVLGMDESVLDVDQIENLIRFCPTKEEMKLLKNYTGDKATLGKCEQYFLELMKVPGVESKLRVFSFKIHFGTQIKELNKGLNTVNSACEEIRTSQKLKEIMKIILCLGNILNQGTARGSAVGFKLDSLLNLSEKCSANTNMTLMHYLCKV 234          

HSP 2 Score: 155.606 bits (392), Expect = 1.530e-38
Identity = 80/138 (57.97%), Postives = 102/138 (73.91%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVR-AHEENCKQ 4595
            HY   V  L  K+ +LL+F KDL  LE+++KI LK LAEEM AI+KGL+K+ QELTASE+DGP+ E F   LK+F+  AE++V +++ LYSSVG N DAL  YFGEDP   PFEQV +TLL+FVR+F   AH+EN KQ
Sbjct:  229 HYLCKV--LASKASDLLDFHKDLENLESASKIHLKSLAEEMVAITKGLQKLNQELTASESDGPISEVFRKLLKDFISVAETQVATVSSLYSSVGGNTDALVHYFGEDPNDYPFEQVTATLLSFVRLFTTAAHQENVKQ 364          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FH19_ARATH (Formin-like protein 19 OS=Arabidopsis thaliana GN=FH19 PE=2 SV=1)

HSP 1 Score: 222.246 bits (565), Expect = 1.790e-60
Identity = 138/262 (52.67%), Postives = 172/262 (65.65%), Query Frame = 3

Query: 3051 KNNLKPYHWLKLTRAMQGSLWAEAQK--------PEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXX-XQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFL---ELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 3800
            K +LKP HW+K TRA+ GSLW E Q+         E+   A E+ +SE+E++FS  A                   +PEKV LI+LRRA N EI L  + I LPD+ ++ + +D++ LD  DQ+ENLI   PTKE+ + L  Y GDK   G CEQ F    E++KVPRVESKLRVF FKIQF +Q++ L  GLN +NS  EE+R S KLK IM+ IL LGN LN+GT RG AVGFRLDSLL L+ETRA N+KMTLMHYLCKV
Sbjct:   75 KCSLKPLHWVKKTRALPGSLWDELQRRQECRDIEDEQILCAIELSVSEIETIFSLGAKPK-------------PKPEPEKVPLIDLRRATNTEIRLMLLNIRLPDMIAAAMAMDESRLDDFDQIENLINLFPTKEDMKFLLTYTGDK---GNCEQLFQYLQEVVKVPRVESKLRVFSFKIQFGTQITKLTKGLNAVNSACEEIRTSQKLKDIMENILCLGNILNQGTGRGRAVGFRLDSLLILSETRADNSKMTLMHYLCKV 320          

HSP 2 Score: 143.665 bits (361), Expect = 3.430e-34
Identity = 77/137 (56.20%), Postives = 99/137 (72.26%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQ 4595
            HY   V  L  K+ +LL+F KDL +LE+++KIQLK LAEE+QAI+KGLEK+ QELTASE DGPV + F   LKEF+  AE++V ++  LY     NA+ALA YFG      PFEQV +TLL+F+R+F +AHEEN KQ
Sbjct:  315 HYLCKV--LASKASDLLDFHKDLESLESASKIQLKSLAEEIQAITKGLEKLKQELTASETDGPVSQVFRKLLKEFISNAETQVATVMALYYPARGNAEALAHYFG---YHYPFEQVTATLLSFIRLFKKAHEENVKQ 446          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FH15A_ARATH (Formin-like protein 15a OS=Arabidopsis thaliana GN=FH15A PE=2 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 4.620e-49
Identity = 117/265 (44.15%), Postives = 162/265 (61.13%), Query Frame = 3

Query: 3048 RKNNLKPYHWLKLTRAMQGSLWAEAQKPE-EAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEE-------VRNSVKLKRIMQTILYLGNALNKGTARG----SAVGFRLDSLLKLTETRARNNKMTLMHYLCKVKD 3806
            ++ +LKP HW+K+T  +QGSLW E Q+   ++  A E+D+SELE+LF   A                   KPEK++L +LRRA      +  + +PLPD+ ++VL +D++ +D DQ+E LIKFCPT EE ELLK Y GDK  LGK EQ+ LEL+KVPR+E+KLRVF FK QF +++++L+  LN++ S  EE       VR+S KLK IM+ I  LGN  N+G  RG    S V F+LD L            +  MHY CK+K+
Sbjct:   58 KRASLKPLHWVKITSDLQGSLWDELQRRHGDSQTAIELDISELETLFFVEA-------------------KPEKIRLHDLRRASYRVFNVINLSMPLPDMMTAVLAMDESVVDVDQIEKLIKFCPTNEEMELLKTYTGDKAALGKYEQYLLELMKVPRLEAKLRVFSFKTQFGTKITELKERLNVVTSACEENLLLIHQVRSSEKLKEIMKKIPCLGNTSNQGPDRGKTFLSPVEFKLDRL-----------SVKRMHYFCKLKE 292          

HSP 2 Score: 73.1738 bits (178), Expect = 9.079e-12
Identity = 38/69 (55.07%), Postives = 50/69 (72.46%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFC 4391
            HY+  V  L  ++ ELL+  KDL +LE+++KIQ+K LA+ +QAI K LEK+ QELTASE DGP  E FC
Sbjct:  328 HYYCKV--LASEASELLDVYKDLQSLESASKIQVKSLAQNIQAIIKRLEKLKQELTASETDGPASEVFC 394          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FH21A_ARATH (Putative formin-like protein 21a OS=Arabidopsis thaliana GN=FH21A PE=3 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 4.178e-44
Identity = 116/275 (42.18%), Postives = 153/275 (55.64%), Query Frame = 3

Query: 3006 RGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQ---------KPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXX-XXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 3800
            +GI C  P      +K++LK ++W+K+TRA+ GSLW E Q         + E+   A E+D+SE+E+ FS  A                   KPEK  LI+LRRA + E+ L  + I LP    + +   D ++ D D++  LI   PTKE  ELL  Y G K  L K EQ+F EL KV RVESKLRVF FKIQFS++++  +  LN++NS  EEV +S KLK IM+ I  LGN  N+GT RG  VGF LDSL            +  MH  CKV
Sbjct:  118 KGIVCCFP--CPSKKKSSLKRFNWVKITRALPGSLWDELQIQQVCHGDIEDEQILCAIELDVSEIETFFSLGA------------------AKPEKDPLIDLRRATDTELTLMLLNIRLPADMMAAIMAMDESVLDDDEIRGLINLFPTKENMELLMSYTGGKWTLEKWEQYFQELRKVLRVESKLRVFYFKIQFSTKITQFKKRLNVVNSACEEVCSSQKLKEIMKKITCLGNTSNQGTGRGVTVGFNLDSLC-----------VKSMHNFCKV 361          

HSP 2 Score: 64.3142 bits (155), Expect = 5.844e-9
Identity = 35/66 (53.03%), Postives = 47/66 (71.21%), Query Frame = 3

Query: 4206 VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETF---CW 4394
            VL  ++ +LL+  KDL +LE+++K QLK LAEEMQ I + LEK+ QELTA+E DGP  + F   CW
Sbjct:  361 VLASEASDLLDVHKDLQSLESASKKQLKSLAEEMQDIIRDLEKLNQELTAAETDGPDSQVFRNVCW 426          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FORH_DICDI (Formin-H OS=Dictyostelium discoideum GN=forH PE=1 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 3.700e-33
Identity = 86/255 (33.73%), Postives = 147/255 (57.65%), Query Frame = 3

Query: 3060 LKPYHWLKL-TRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFR-LDSLLKLTETRARNNKMTLMHYLCK-VKDHLP 3815
            ++ ++W+ +    +QG+ W    K +E      +D  ELESLFSA AP     S+           +   V +I++++A NC IML   KIP   L    + LD+     +    L++F PTKE+ E +K Y GD+ +LG  EQ+ L ++ +P+++S+L+ F+FK +F   V DL   +  I + + E++ S +L  I++ IL +GN +N  T RG A GF+ L++L K+ + R+ +NK++L+H+L K ++D +P
Sbjct:  633 MRNFNWITIPALKVQGTFW---DKLDETSFIQSLDKVELESLFSAKAPTVKVESK--------QLTRKVVVTVIDMKKANNCAIMLQHFKIPNEQLKKMQIMLDEKHFSQENAIYLLQFAPTKEDIEAIKEYQGDQMQLGAAEQYMLTVMDIPKLDSRLKAFIFKQKFEGLVEDLVPDIKAIKAASLELKKSKRLSDILKFILAIGNYVNGSTTRGGAFGFKVLETLPKMRDARSNDNKLSLLHFLAKTLQDRIP 876          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FH12_ARATH (Formin-like protein 12 OS=Arabidopsis thaliana GN=FH12 PE=2 SV=2)

HSP 1 Score: 130.954 bits (328), Expect = 4.317e-31
Identity = 84/173 (48.55%), Postives = 114/173 (65.90%), Query Frame = 3

Query: 3291 AYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKG-YNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKVKD 3806
            A NCE ML+K+KIPLPD+ ++VL LD + +  DQ++NLIK C +KEE + L+    GDKE LGKCE+ F EL+ VPR+E KLRVF FK+++ S+VSDL+  ++ I +  +E+  SVKL RIMQT      +L     RGS V   LDSL+KL +       + LMH  CK+ D
Sbjct:    2 ASNCEKMLSKIKIPLPDMLNAVLDLDSSAVIIDQIKNLIKICWSKEEMDRLRNSAGGDKEVLGKCEEIFGELMMVPRIEPKLRVFAFKVEYPSRVSDLKMWMHTIIAATKEITGSVKLFRIMQT------SLTMQVLRGSNVECGLDSLVKLCDN------VYLMHDFCKLLD 162          

HSP 2 Score: 68.9366 bits (167), Expect = 6.930e-11
Identity = 46/138 (33.33%), Postives = 74/138 (53.62%), Query Frame = 3

Query: 4230 LLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEK 4643
            LL+F  DLV LEA+++I+L+ +  +MQ +    E+V  E  ASENDG     +   + +FL   + + + L +LY+ VG   ++   Y  E P+   F++  + L  FV  F ++ EE  +Q E EK   +K   N K
Sbjct:  160 LLDFGNDLVHLEAASRIELETITNKMQELFDIEEEVNDEFLASENDGANFVGYRNVVHDFLCTIDGDKQLLNILYAEVGGLVNS---YIAEYPSGVRFKEATNILTRFVETFYKSREEIERQAEAEKEILEKRKMNIK 294          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: DAAM1_HUMAN (Disheveled-associated activator of morphogenesis 1 OS=Homo sapiens GN=DAAM1 PE=1 SV=2)

HSP 1 Score: 126.331 bits (316), Expect = 1.037e-27
Identity = 89/283 (31.45%), Postives = 165/283 (58.30%), Query Frame = 3

Query: 3018 CIGPKSLAQPRKNNLKPYHWLKL-TRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSA--------AAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXX-XXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNK-MTLMHYLCK-VKDHLPFLLSL 3830
             +  KS+ QP  N LK ++W KL    ++G++W E    +  FK   +D+ +LE  FSA           NS     D   +  +S  K +++ +I+ RRA NC I+L+++K+   ++  ++LT+D+      D LE L+KF P K + +LL+ +  + +++ K ++F  E+ ++   + +L+   FK +F+ +V++++  +  I S +EEV  S  LK++++ +L  GN +NKG  RG+A GF++ SL K+ +T++  +K +TL+HYL   V++  P +L+L
Sbjct:  597 ALKKKSIPQP-TNALKSFNWSKLPENKLEGTVWTEIDDTK-VFKI--LDLEDLERTFSAYQRQQDFFVNSNSKQKEADAIDDTLSSKLKVKELSVIDGRRAQNCNILLSRLKLSNDEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSGSEEVFRSGALKQLLEVVLAFGNYMNKG-QRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPSVLNL 874          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy Swiss-Prot
Match: FORF_DICDI (Formin-F OS=Dictyostelium discoideum GN=forF PE=1 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 4.209e-27
Identity = 82/278 (29.50%), Postives = 145/278 (52.16%), Query Frame = 3

Query: 3024 GPKSLAQPRKNNLKPYHWLKL-TRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGY---NGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLC-----KVKDHLPFLLSL 3830
             PK         +K + W K+  + +  +++          K   +++ E+E+LF A   NS     +    +  S  KP  V +I+ +++ N  I L+K K PL ++ +++ TLD+     + L+ L ++ PT E+ E +K Y   +G+ + L K E F LE+  V  +  +++ F  KI F  ++ +++  L +   T ++++NS    ++M+ +L +GN LN GTARG   GF+LD+LLKL +T+  NNK  L+ Y+      K  D L F+  L
Sbjct:  654 APKFTVSKPTTKVKQFQWTKIPNKKLGETIFTNLG----TIKTDWLNVGEIENLFFAPEANS-QKKLEASDKKSTSSTKPGTVSVIDPKKSQNLAIYLSKFKCPLEEIKTALYTLDEDIFTMESLKALEQYLPTDEDMEAIKDYLKKDGELKMLTKAEHFLLEMDSVSSLAERVKSFYLKILFPDKLKEIKPDLELFTKTIKDIKNSKNFLKVMEVVLIIGNFLNGGTARGDCFGFKLDALLKLADTKTANNKSNLLVYIISELEQKFPDSLKFMDDL 926          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: A0A0D2RV42_GOSRA (Formin-like protein OS=Gossypium raimondii GN=B456_009G062500 PE=3 SV=1)

HSP 1 Score: 1765.36 bits (4571), Expect = 0.000e+0
Identity = 1118/1163 (96.13%), Postives = 1125/1163 (96.73%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLETGCPKSREIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESEDXXXXXXXXXXXXXXXXXXXXTPKHLQLTRAIAXXXXXXXXXXXXXXXXXXXXXXXXYQXXXXXXXXXXXXXXXFQSPPYPPTPLHSFSRASPSPLVKNXXXXXXXXXXAFLAPKVENLCQXXXXXXXXXXXXXXXXXXXVKNXXXXXXXXXXXXXX--XXXXXXXXXXXXXXXXXXXXXXXXXXXXXTCPNSCSPQIRTSGXXXXXXXXXQSGSISDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCAKGLSKAGDTSPQLHSVSXXXXXXXXXXXXXXXLNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 3800
            MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEY MTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTS LNPLPSQLRYLQYVSRRNV SEWPPSDKALTLDCIILRCIPNMDGEGGCR IFRIYGQDPFMAADRTPKVLFSTPK SKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDF+AEVIFSDIDVAPSAISIDFP IEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENL+TGCPKS+EIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQ SDANSTRTKFEPEEIQV PQMATQH+PSAVPS DSSSTEGKTESQELQVA QEAELTN SRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPA ISRY SP SALGITALLHDHDASESEDHSHPVAS LSSSSVSS LS+TPKHLQLTRAIASPGQSSFVPPPPPPLPSFSGASPAYQP PSLEVSPPSVKSSFQSPP+PP PLHSFSR SPSPLVKNSLQ LPPS SA LAPKVENL +SPPPPPPLPPSSATSPSSSVKNSFSAPPPPPP P    SAISP  TV NSSSA PPPPPLPNVASSTTCPNSCSPQIRTSGPPPPPAPPPQSGSISDPTSKSSVPRP PPPPPSLAAKGPS NNSAHVPPVPPPPAPCAKGLSKAGDTSPQLHSVSNGNIPSIPG PSAAPLNLKGRGIS IGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEA KAPEIDMSELESLFSAAAPNSDN SRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPDLTSSVLTLDDTTLDSDQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV
Sbjct:    1 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYDMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSPLNPLPSQLRYLQYVSRRNVGSEWPPSDKALTLDCIILRCIPNMDGEGGCRLIFRIYGQDPFMAADRTPKVLFSTPKNSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFSAEVIFSDIDVAPSAISIDFPDIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLKTGCPKSKEIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQSSDANSTRTKFEPEEIQVAPQMATQHSPSAVPSSDSSSTEGKTESQELQVALQEAELTNPSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAPISRYRSPPSALGITALLHDHDASESEDHSHPVASSLSSSSVSSHLSNTPKHLQLTRAIASPGQSSFVPPPPPPLPSFSGASPAYQPPPSLEVSPPSVKSSFQSPPHPPPPLHSFSRESPSPLVKNSLQSLPPSSSASLAPKVENLFRSPPPPPPLPPSSATSPSSSVKNSFSAPPPPPPPPLPPFSAISPSFTVKNSSSAPPPPPPLPNVASSTTCPNSCSPQIRTSGPPPPPAPPPQSGSISDPTSKSSVPRPSPPPPPSLAAKGPSPNNSAHVPPVPPPPAPCAKGLSKAGDTSPQLHSVSNGNIPSIPGQPSAAPLNLKGRGISRIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEASKAPEIDMSELESLFSAAAPNSDNASRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPDLTSSVLTLDDTTLDSDQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 1163          

HSP 2 Score: 168.318 bits (425), Expect = 1.450e-38
Identity = 86/93 (92.47%), Postives = 86/93 (92.47%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSV 4463
            HY   V  LNEKSPELLEF KDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPV ETFCWTLKEFLGFAESEVRSLALLYSSV
Sbjct: 1158 HYLCKV--LNEKSPELLEFPKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVSETFCWTLKEFLGFAESEVRSLALLYSSV 1248          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: A0A0D2QA64_GOSRA (Formin-like protein OS=Gossypium raimondii GN=B456_009G062500 PE=3 SV=1)

HSP 1 Score: 1764.59 bits (4569), Expect = 0.000e+0
Identity = 1118/1163 (96.13%), Postives = 1125/1163 (96.73%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLETGCPKSREIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESEDXXXXXXXXXXXXXXXXXXXXTPKHLQLTRAIAXXXXXXXXXXXXXXXXXXXXXXXXYQXXXXXXXXXXXXXXXFQSPPYPPTPLHSFSRASPSPLVKNXXXXXXXXXXAFLAPKVENLCQXXXXXXXXXXXXXXXXXXXVKNXXXXXXXXXXXXXX--XXXXXXXXXXXXXXXXXXXXXXXXXXXXXTCPNSCSPQIRTSGXXXXXXXXXQSGSISDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCAKGLSKAGDTSPQLHSVSXXXXXXXXXXXXXXXLNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 3800
            MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEY MTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTS LNPLPSQLRYLQYVSRRNV SEWPPSDKALTLDCIILRCIPNMDGEGGCR IFRIYGQDPFMAADRTPKVLFSTPK SKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDF+AEVIFSDIDVAPSAISIDFP IEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENL+TGCPKS+EIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQ SDANSTRTKFEPEEIQV PQMATQH+PSAVPS DSSSTEGKTESQELQVA QEAELTN SRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPA ISRY SP SALGITALLHDHDASESEDHSHPVAS LSSSSVSS LS+TPKHLQLTRAIASPGQSSFVPPPPPPLPSFSGASPAYQP PSLEVSPPSVKSSFQSPP+PP PLHSFSR SPSPLVKNSLQ LPPS SA LAPKVENL +SPPPPPPLPPSSATSPSSSVKNSFSAPPPPPP P    SAISP  TV NSSSA PPPPPLPNVASSTTCPNSCSPQIRTSGPPPPPAPPPQSGSISDPTSKSSVPRP PPPPPSLAAKGPS NNSAHVPPVPPPPAPCAKGLSKAGDTSPQLHSVSNGNIPSIPG PSAAPLNLKGRGIS IGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEA KAPEIDMSELESLFSAAAPNSDN SRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPDLTSSVLTLDDTTLDSDQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV
Sbjct:    1 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYDMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSPLNPLPSQLRYLQYVSRRNVGSEWPPSDKALTLDCIILRCIPNMDGEGGCRLIFRIYGQDPFMAADRTPKVLFSTPKNSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFSAEVIFSDIDVAPSAISIDFPDIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLKTGCPKSKEIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQSSDANSTRTKFEPEEIQVAPQMATQHSPSAVPSSDSSSTEGKTESQELQVALQEAELTNPSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAPISRYRSPPSALGITALLHDHDASESEDHSHPVASSLSSSSVSSHLSNTPKHLQLTRAIASPGQSSFVPPPPPPLPSFSGASPAYQPPPSLEVSPPSVKSSFQSPPHPPPPLHSFSRESPSPLVKNSLQSLPPSSSASLAPKVENLFRSPPPPPPLPPSSATSPSSSVKNSFSAPPPPPPPPLPPFSAISPSFTVKNSSSAPPPPPPLPNVASSTTCPNSCSPQIRTSGPPPPPAPPPQSGSISDPTSKSSVPRPSPPPPPSLAAKGPSPNNSAHVPPVPPPPAPCAKGLSKAGDTSPQLHSVSNGNIPSIPGQPSAAPLNLKGRGISRIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEASKAPEIDMSELESLFSAAAPNSDNASRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPDLTSSVLTLDDTTLDSDQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 1163          

HSP 2 Score: 301.982 bits (772), Expect = 2.842e-80
Identity = 150/161 (93.17%), Postives = 152/161 (94.41%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVSTPQR 4667
            HY   V  LNEKSPELLEF KDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPV ETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEK KAQKEA+NEKLKVSTP +
Sbjct: 1158 HYLCKV--LNEKSPELLEFPKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVSETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKKKAQKEADNEKLKVSTPTK 1316          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: A0A0D2TEN7_GOSRA (Formin-like protein OS=Gossypium raimondii GN=B456_009G062500 PE=3 SV=1)

HSP 1 Score: 1764.2 bits (4568), Expect = 0.000e+0
Identity = 1118/1163 (96.13%), Postives = 1125/1163 (96.73%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLETGCPKSREIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESEDXXXXXXXXXXXXXXXXXXXXTPKHLQLTRAIAXXXXXXXXXXXXXXXXXXXXXXXXYQXXXXXXXXXXXXXXXFQSPPYPPTPLHSFSRASPSPLVKNXXXXXXXXXXAFLAPKVENLCQXXXXXXXXXXXXXXXXXXXVKNXXXXXXXXXXXXXX--XXXXXXXXXXXXXXXXXXXXXXXXXXXXXTCPNSCSPQIRTSGXXXXXXXXXQSGSISDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCAKGLSKAGDTSPQLHSVSXXXXXXXXXXXXXXXLNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 3800
            MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEY MTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTS LNPLPSQLRYLQYVSRRNV SEWPPSDKALTLDCIILRCIPNMDGEGGCR IFRIYGQDPFMAADRTPKVLFSTPK SKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDF+AEVIFSDIDVAPSAISIDFP IEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENL+TGCPKS+EIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQ SDANSTRTKFEPEEIQV PQMATQH+PSAVPS DSSSTEGKTESQELQVA QEAELTN SRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPA ISRY SP SALGITALLHDHDASESEDHSHPVAS LSSSSVSS LS+TPKHLQLTRAIASPGQSSFVPPPPPPLPSFSGASPAYQP PSLEVSPPSVKSSFQSPP+PP PLHSFSR SPSPLVKNSLQ LPPS SA LAPKVENL +SPPPPPPLPPSSATSPSSSVKNSFSAPPPPPP P    SAISP  TV NSSSA PPPPPLPNVASSTTCPNSCSPQIRTSGPPPPPAPPPQSGSISDPTSKSSVPRP PPPPPSLAAKGPS NNSAHVPPVPPPPAPCAKGLSKAGDTSPQLHSVSNGNIPSIPG PSAAPLNLKGRGIS IGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEA KAPEIDMSELESLFSAAAPNSDN SRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPDLTSSVLTLDDTTLDSDQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV
Sbjct:    1 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYDMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSPLNPLPSQLRYLQYVSRRNVGSEWPPSDKALTLDCIILRCIPNMDGEGGCRLIFRIYGQDPFMAADRTPKVLFSTPKNSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFSAEVIFSDIDVAPSAISIDFPDIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLKTGCPKSKEIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQSSDANSTRTKFEPEEIQVAPQMATQHSPSAVPSSDSSSTEGKTESQELQVALQEAELTNPSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAPISRYRSPPSALGITALLHDHDASESEDHSHPVASSLSSSSVSSHLSNTPKHLQLTRAIASPGQSSFVPPPPPPLPSFSGASPAYQPPPSLEVSPPSVKSSFQSPPHPPPPLHSFSRESPSPLVKNSLQSLPPSSSASLAPKVENLFRSPPPPPPLPPSSATSPSSSVKNSFSAPPPPPPPPLPPFSAISPSFTVKNSSSAPPPPPPLPNVASSTTCPNSCSPQIRTSGPPPPPAPPPQSGSISDPTSKSSVPRPSPPPPPSLAAKGPSPNNSAHVPPVPPPPAPCAKGLSKAGDTSPQLHSVSNGNIPSIPGQPSAAPLNLKGRGISRIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEASKAPEIDMSELESLFSAAAPNSDNASRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPDLTSSVLTLDDTTLDSDQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 1163          

HSP 2 Score: 285.419 bits (729), Expect = 5.835e-75
Identity = 142/153 (92.81%), Postives = 144/153 (94.12%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEK 4643
            HY   V  LNEKSPELLEF KDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPV ETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEK KAQKE+E+ K
Sbjct: 1158 HYLCKV--LNEKSPELLEFPKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVSETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKKKAQKESEHLK 1308          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: A0A0D2TKK1_GOSRA (Formin-like protein OS=Gossypium raimondii GN=B456_009G062500 PE=3 SV=1)

HSP 1 Score: 1687.93 bits (4370), Expect = 0.000e+0
Identity = 1089/1163 (93.64%), Postives = 1096/1163 (94.24%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLETGCPKSREIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESEDXXXXXXXXXXXXXXXXXXXXTPKHLQLTRAIAXXXXXXXXXXXXXXXXXXXXXXXXYQXXXXXXXXXXXXXXXFQSPPYPPTPLHSFSRASPSPLVKNXXXXXXXXXXAFLAPKVENLCQXXXXXXXXXXXXXXXXXXXVKNXXXXXXXXXXXXXX--XXXXXXXXXXXXXXXXXXXXXXXXXXXXXTCPNSCSPQIRTSGXXXXXXXXXQSGSISDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCAKGLSKAGDTSPQLHSVSXXXXXXXXXXXXXXXLNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 3800
            MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEY MTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTS LNPLPSQLRYLQYVSRRNV SEWPPSDK                              DPFMAADRTPKVLFSTPK SKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDF+AEVIFSDIDVAPSAISIDFP IEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENL+TGCPKS+EIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQ SDANSTRTKFEPEEIQV PQMATQH+PSAVPS DSSSTEGKTESQELQVA QEAELTN SRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPA ISRY SP SALGITALLHDHDASESEDHSHPVAS LSSSSVSS LS+TPKHLQLTRAIASPGQSSFVPPPPPPLPSFSGASPAYQP PSLEVSPPSVKSSFQSPP+PP PLHSFSR SPSPLVKNSLQ LPPS SA LAPKVENL +SPPPPPPLPPSSATSPSSSVKNSFSAPPPPPP P    SAISP  TV NSSSA PPPPPLPNVASSTTCPNSCSPQIRTSGPPPPPAPPPQSGSISDPTSKSSVPRP PPPPPSLAAKGPS NNSAHVPPVPPPPAPCAKGLSKAGDTSPQLHSVSNGNIPSIPG PSAAPLNLKGRGIS IGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEA KAPEIDMSELESLFSAAAPNSDN SRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPDLTSSVLTLDDTTLDSDQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV
Sbjct:    1 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYDMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSPLNPLPSQLRYLQYVSRRNVGSEWPPSDK------------------------------DPFMAADRTPKVLFSTPKNSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFSAEVIFSDIDVAPSAISIDFPDIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLKTGCPKSKEIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQSSDANSTRTKFEPEEIQVAPQMATQHSPSAVPSSDSSSTEGKTESQELQVALQEAELTNPSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAPISRYRSPPSALGITALLHDHDASESEDHSHPVASSLSSSSVSSHLSNTPKHLQLTRAIASPGQSSFVPPPPPPLPSFSGASPAYQPPPSLEVSPPSVKSSFQSPPHPPPPLHSFSRESPSPLVKNSLQSLPPSSSASLAPKVENLFRSPPPPPPLPPSSATSPSSSVKNSFSAPPPPPPPPLPPFSAISPSFTVKNSSSAPPPPPPLPNVASSTTCPNSCSPQIRTSGPPPPPAPPPQSGSISDPTSKSSVPRPSPPPPPSLAAKGPSPNNSAHVPPVPPPPAPCAKGLSKAGDTSPQLHSVSNGNIPSIPGQPSAAPLNLKGRGISRIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEASKAPEIDMSELESLFSAAAPNSDNASRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPDLTSSVLTLDDTTLDSDQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 1133          

HSP 2 Score: 302.753 bits (774), Expect = 1.563e-80
Identity = 150/161 (93.17%), Postives = 152/161 (94.41%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVSTPQR 4667
            HY   V  LNEKSPELLEF KDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPV ETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEK KAQKEA+NEKLKVSTP +
Sbjct: 1128 HYLCKV--LNEKSPELLEFPKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVSETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKKKAQKEADNEKLKVSTPTK 1286          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: A0A0D2UH83_GOSRA (Formin-like protein OS=Gossypium raimondii GN=B456_009G062500 PE=3 SV=1)

HSP 1 Score: 1686 bits (4365), Expect = 0.000e+0
Identity = 1089/1163 (93.64%), Postives = 1096/1163 (94.24%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLETGCPKSREIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESEDXXXXXXXXXXXXXXXXXXXXTPKHLQLTRAIAXXXXXXXXXXXXXXXXXXXXXXXXYQXXXXXXXXXXXXXXXFQSPPYPPTPLHSFSRASPSPLVKNXXXXXXXXXXAFLAPKVENLCQXXXXXXXXXXXXXXXXXXXVKNXXXXXXXXXXXXXX--XXXXXXXXXXXXXXXXXXXXXXXXXXXXXTCPNSCSPQIRTSGXXXXXXXXXQSGSISDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCAKGLSKAGDTSPQLHSVSXXXXXXXXXXXXXXXLNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 3800
            MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEY MTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTS LNPLPSQLRYLQYVSRRNV SEWPPSDK                              DPFMAADRTPKVLFSTPK SKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDF+AEVIFSDIDVAPSAISIDFP IEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENL+TGCPKS+EIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQ SDANSTRTKFEPEEIQV PQMATQH+PSAVPS DSSSTEGKTESQELQVA QEAELTN SRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPA ISRY SP SALGITALLHDHDASESEDHSHPVAS LSSSSVSS LS+TPKHLQLTRAIASPGQSSFVPPPPPPLPSFSGASPAYQP PSLEVSPPSVKSSFQSPP+PP PLHSFSR SPSPLVKNSLQ LPPS SA LAPKVENL +SPPPPPPLPPSSATSPSSSVKNSFSAPPPPPP P    SAISP  TV NSSSA PPPPPLPNVASSTTCPNSCSPQIRTSGPPPPPAPPPQSGSISDPTSKSSVPRP PPPPPSLAAKGPS NNSAHVPPVPPPPAPCAKGLSKAGDTSPQLHSVSNGNIPSIPG PSAAPLNLKGRGIS IGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEA KAPEIDMSELESLFSAAAPNSDN SRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPDLTSSVLTLDDTTLDSDQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV
Sbjct:    1 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYDMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSPLNPLPSQLRYLQYVSRRNVGSEWPPSDK------------------------------DPFMAADRTPKVLFSTPKNSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFSAEVIFSDIDVAPSAISIDFPDIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLKTGCPKSKEIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQSSDANSTRTKFEPEEIQVAPQMATQHSPSAVPSSDSSSTEGKTESQELQVALQEAELTNPSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAPISRYRSPPSALGITALLHDHDASESEDHSHPVASSLSSSSVSSHLSNTPKHLQLTRAIASPGQSSFVPPPPPPLPSFSGASPAYQPPPSLEVSPPSVKSSFQSPPHPPPPLHSFSRESPSPLVKNSLQSLPPSSSASLAPKVENLFRSPPPPPPLPPSSATSPSSSVKNSFSAPPPPPPPPLPPFSAISPSFTVKNSSSAPPPPPPLPNVASSTTCPNSCSPQIRTSGPPPPPAPPPQSGSISDPTSKSSVPRPSPPPPPSLAAKGPSPNNSAHVPPVPPPPAPCAKGLSKAGDTSPQLHSVSNGNIPSIPGQPSAAPLNLKGRGISRIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEASKAPEIDMSELESLFSAAAPNSDNASRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPDLTSSVLTLDDTTLDSDQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 1133          

HSP 2 Score: 222.246 bits (565), Expect = 3.673e-55
Identity = 110/123 (89.43%), Postives = 112/123 (91.06%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNF 4553
            HY   V  LNEKSPELLEF KDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPV ETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQV+  L+  
Sbjct: 1128 HYLCKV--LNEKSPELLEFPKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVSETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVLFDLVKL 1248          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: A0A0B0PUI5_GOSAR (Formin-like protein OS=Gossypium arboreum GN=F383_03986 PE=3 SV=1)

HSP 1 Score: 1685.62 bits (4364), Expect = 0.000e+0
Identity = 1066/1101 (96.82%), Postives = 1067/1101 (96.91%), Query Frame = 3

Query:  498 MVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLETGCPKSREIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESEDXXXXXXXXXXXXXXXXXXXXTPKHLQLTRAIAXXXXXXXXXXXXXXXXXXXXXXXXYQXXXXXXXXXXXXXXXFQSPPYPPTPLHSFSRASPSPLVKNXXXXXXXXXXAFLAPKVENLCQXXXXXXXXXXXXXXXXXXXVKNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTCPNSCSPQIRTSGXXXXXXXXXQSGSISDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCAKGLSKAGDTSPQLHSVSXXXXXXXXXXXXXXXLNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 3800
            MVFNFREGEYQSQIASILSEY MTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLL RKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPK SKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVA SAISIDFPG EEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLETGCPKSREIASVLHDDKVEEKSNSEAP HVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRY SPLSALGITALLHDHDASESEDHSHPVASPLS SSVSSPLS+TPKHLQLTRAIASPGQSSFVPPPPPPLPSFSGASPAYQPSPSLEVSPPSVKSSFQSPPYPPTPLHSFSRASPSPLVKNSLQ LPPSLSA LAPKVENLCQSPPPPPPLPPSSATSPSSSVKNSFSAPPPPPPLPPFSAISP STVNNSSSASPPPPPLPNVASSTTCPNSCSPQIRTSGPPPPPAPPPQS SISD TSKSSVPRPPPPPPPSLAAKGPSSNNSAHVPPVPPPPAPCAKGLSKAGDTSPQLHSVSNGNIPSIPGPPSAAPLNLKGR IS IGPKSLAQPRK NLKPYHWLKLTRAMQGSLWAEAQKPEEA KAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPDLTSSVLTLDDTTLDSDQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQ                 VRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV
Sbjct:    1 MVFNFREGEYQSQIASILSEYDMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLNRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKNSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVATSAISIDFPGFEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLETGCPKSREIASVLHDDKVEEKSNSEAPAHVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYRSPLSALGITALLHDHDASESEDHSHPVASPLSFSSVSSPLSNTPKHLQLTRAIASPGQSSFVPPPPPPLPSFSGASPAYQPSPSLEVSPPSVKSSFQSPPYPPTPLHSFSRASPSPLVKNSLQSLPPSLSASLAPKVENLCQSPPPPPPLPPSSATSPSSSVKNSFSAPPPPPPLPPFSAISPSSTVNNSSSASPPPPPLPNVASSTTCPNSCSPQIRTSGPPPPPAPPPQSASISDTTSKSSVPRPPPPPPPSLAAKGPSSNNSAHVPPVPPPPAPCAKGLSKAGDTSPQLHSVSNGNIPSIPGPPSAAPLNLKGRAISRIGPKSLAQPRKANLKPYHWLKLTRAMQGSLWAEAQKPEEASKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPDLTSSVLTLDDTTLDSDQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQ-----------------VRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV 1084          

HSP 2 Score: 312.383 bits (799), Expect = 1.009e-83
Identity = 154/161 (95.65%), Postives = 155/161 (96.27%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVSTPQR 4667
            HY   V  LNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVSTP +
Sbjct: 1079 HYLCKV--LNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVSTPTK 1237          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: A0A061GJK1_THECC (Formin-like protein OS=Theobroma cacao GN=TCM_036911 PE=3 SV=1)

HSP 1 Score: 937.176 bits (2421), Expect = 0.000e+0
Identity = 484/657 (73.67%), Postives = 537/657 (81.74%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDY----TDVANM---------LQENLETGCPKSREIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLS-DANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELT----------NSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESEDXXXXXXXXXXXXXXXXXXXXTPKHLQLTRAI 2216
            M LFRK F+RKPPDGLLEISERVYVFDCCFS DI E+ EY+ YI  IV +LRDHF DASFMVFNFREGEYQSQIASILSEY MTVMDYPRQYEGCPLLT E++HHFL+SSE+W SLGQQN+ILMH ERGG PVLAFML ALL+YRKQ++GEQKTL+M YKQAPRELL L S LNPLPSQLRYLQYVSRRN+ SEWPP+D+ALTLDCII+R IPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSK V+YYKQ DCELVKI+I CHV+GDVVLECISLDS+  RE M+FR+MFNTAFIRSNILMLN +EIDILWN KDQFPKDF AEVIFS++DVA S +SID PGIEEK+GL ++ FAKVQEIFS VD+     DVANM         LQEN ETG  K  E ASVLHDD+V+EKSN +A EH+ TSPTS ALEKQS  S + S DANSTR KFEP+EIQV P+   Q  PS+  S D S  EG TE  ELQVAPQE + +          NS RR VE QE+Q++ QQPAQS S+F P  +VNSIRKK EPQELQVA+QRPAQPKIISQR PQSSIS PVSYCNSL GSP  ISRYHS  SALGITALLHDH  S+SE+  HPV  PLSSS++SSPL +TPK LQ + AI
Sbjct:    1 MALFRKFFYRKPPDGLLEISERVYVFDCCFSKDIWEDDEYKTYIGGIVGQLRDHFPDASFMVFNFREGEYQSQIASILSEYDMTVMDYPRQYEGCPLLTMEMVHHFLRSSESWLSLGQQNVILMHCERGGWPVLAFMLAALLIYRKQFTGEQKTLDMTYKQAPRELLQLMSPLNPLPSQLRYLQYVSRRNLGSEWPPADRALTLDCIIIRFIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKLVRYYKQVDCELVKIDIHCHVLGDVVLECISLDSDQARESMMFRVMFNTAFIRSNILMLNLEEIDILWNAKDQFPKDFRAEVIFSEMDVATSVMSIDLPGIEEKDGLPMEAFAKVQEIFSNVDWPSPKRDVANMLQHISVSNILQENSETGISKRMERASVLHDDEVKEKSNVQASEHMITSPTSAALEKQSTFSVKPSLDANSTRKKFEPQEIQVAPRQPAQSIPSSGSSSDPSPMEGLTELHELQVAPQEPKHSKTSNKPSPEANSIRRKVETQELQIALQQPAQSISSFKPTPNVNSIRKKIEPQELQVALQRPAQPKIISQRVPQSSISVPVSYCNSLQGSPVPISRYHSAPSALGITALLHDHVVSKSEECIHPVTLPLSSSAISSPLPNTPKPLQPSLAI 657          

HSP 2 Score: 482.641 bits (1241), Expect = 6.286e-140
Identity = 275/313 (87.86%), Postives = 284/313 (90.73%), Query Frame = 3

Query: 2889 CAKGLSKAGDTSPQLHSVSXXXXXXXXXXXXXXXLNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV-KDHLPFLL 3824
            CAKGLSKAG TSPQ HSVSNGNIPSIPGPPS APLNLKGRGIS IG K+ A  RK NLKPYHWLKLTRAMQGSLWAEAQKPEEA KAPE DMSELESLFSAAAPNSDN  RDGK+NR ASGRK EKVQLIELRRAYNCEIMLTKVKIPLPDL SSVL LDDT LD+DQ+ENLIKFCPTKEE ELLKGYNGDKEKLGKCEQFFLEL+KVPRVESKLRVF FKIQF SQVSDLRN LNI+NS AEEVRNSVKLKRIMQTIL LGNALN GTARGSA+GFRLDSLLKLT+TRARNNKMTLMHYLCKV  + LP LL
Sbjct:  938 CAKGLSKAGGTSPQSHSVSNGNIPSIPGPPSGAPLNLKGRGISRIGSKTQAS-RKTNLKPYHWLKLTRAMQGSLWAEAQKPEEASKAPEFDMSELESLFSAAAPNSDNSIRDGKANRCASGRKSEKVQLIELRRAYNCEIMLTKVKIPLPDLMSSVLALDDTALDADQVENLIKFCPTKEEMELLKGYNGDKEKLGKCEQFFLELMKVPRVESKLRVFSFKIQFCSQVSDLRNSLNIVNSAAEEVRNSVKLKRIMQTILSLGNALNHGTARGSAIGFRLDSLLKLTDTRARNNKMTLMHYLCKVLVEKLPELL 1249          

HSP 3 Score: 268.855 bits (686), Expect = 1.795e-69
Identity = 137/161 (85.09%), Postives = 142/161 (88.20%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVSTPQR 4667
            HY   V  L EK PELL+F KDLVTLE+STKIQLKCLAEEMQAISKGLEKVVQELTASENDGPV ETFC TLKEFL FAE EVRSLA LYS VGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAH+EN KQLELEK KA KEAEN KLK+STP +
Sbjct: 1235 HYLCKV--LVEKLPELLDFPKDLVTLESSTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVSETFCRTLKEFLSFAEGEVRSLASLYSCVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHKENSKQLELEKKKALKEAENGKLKISTPPK 1393          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: U5FFI3_POPTR (Formin-like protein OS=Populus trichocarpa GN=POPTR_0018s02490g PE=3 SV=1)

HSP 1 Score: 729.554 bits (1882), Expect = 0.000e+0
Identity = 379/617 (61.43%), Postives = 455/617 (73.74%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDY----TDVANML-------QENLETGCPKSREIASVLHDD---KVEEKSNSEAPEHVTTSPTSDALEKQSICSFQ-LSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESED 2123
            M LFRK F+RKPPDGLLEISERVYVFDCC+  D  EE+EY+VYI  IV KLR+H  DASFMVFNF+EGE +SQI S+LSE+ MTVMDYPR YE  PLL+ E+IHH L+SSE+W SLGQQN++LMH ERGG PVLAFML ALLLY KQ++GEQ+TL+MIYKQ P+ELLHL S +NPLPSQLRYLQYVSRRN+ ++WPP D+ALTLDCIILR IP MD EGGC+PIFRI+GQDPFM  DRTPKVLFSTPK+S+FVQ+YKQADCELVKI+I CH+ GDVV+ECI+LDS+ ERE M+FR+MFNT+FI SNILMLNRDEID LW+ KDQFPKDF AEV+FS++D +    +ID PG+EEK+G+ ++ F +V EIFS +D+    TDVA  +       QENL++  P+  E  SVL +    +V+EK      E+ T SPTS  L KQS  SF+  SD NS R + EP+E++V    A Q  PS   S D++S   K E QEL+VA                           QST +  P LD  S +KK EPQELQVA+Q PAQ KIISQR PQ S+ SPVSY N+L G P  +SRYHS  SALGITALLHDH  S+ E+
Sbjct:    1 MALFRKFFYRKPPDGLLEISERVYVFDCCYCMDTFEEEEYKVYIRGIVGKLRNHLPDASFMVFNFQEGENESQIGSVLSEFDMTVMDYPRHYESFPLLSMEMIHHSLRSSESWLSLGQQNVVLMHCERGGWPVLAFMLAALLLYGKQFTGEQRTLDMIYKQGPQELLHLMSPINPLPSQLRYLQYVSRRNMGTQWPPLDRALTLDCIILRVIPCMDTEGGCQPIFRIHGQDPFMVVDRTPKVLFSTPKRSRFVQHYKQADCELVKIDINCHIQGDVVMECINLDSDHEREQMMFRVMFNTSFISSNILMLNRDEIDTLWDAKDQFPKDFRAEVLFSEMDSSTPIGAIDLPGLEEKDGIPVEVFPRVHEIFSNMDWPDTKTDVAQNMHHHIMEPQENLDSS-PQRAEGGSVLMESTFVRVQEKPKLNESENKTPSPTSITLVKQSTLSFKPFSDTNSVREEAEPQELKV----ALQSMPSIKSSPDANSAREKVEPQELKVA--------------------------CQSTLSIKPSLDAISTQKKVEPQELQVALQWPAQSKIISQRVPQRSLCSPVSYGNNLQGLPVPMSRYHSAPSALGITALLHDHAVSKGEE 586          

HSP 2 Score: 213.001 bits (541), Expect = 2.272e-52
Identity = 109/148 (73.65%), Postives = 120/148 (81.08%), Query Frame = 3

Query: 3390 QLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV-KDHLPFLLSL 3830
            Q+++LIKFCPTKEE ELLKGYNGDKE LGKCEQFFLE++KVPR ESKLRVF FKIQF SQV      L  +      +R+S KLKRIMQTIL LGNALN GTARGSAVGFRLDSLLKLT+TRARNNK+TLMHYLCKV  + LP LL  
Sbjct:  990 QVDSLIKFCPTKEEMELLKGYNGDKENLGKCEQFFLEMMKVPRAESKLRVFSFKIQFQSQVRGQSLSLLFV------IRSSAKLKRIMQTILSLGNALNHGTARGSAVGFRLDSLLKLTDTRARNNKITLMHYLCKVLAEKLPELLDF 1131          

HSP 3 Score: 89.3521 bits (220), Expect = 1.993e-14
Identity = 51/79 (64.56%), Postives = 58/79 (73.42%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKI----------QLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFC 4391
            HY   V  L EK PELL+F+KDLV LEA+TK+          QLK LAEEMQA+SKGLEKV+QELTASENDG V E+FC
Sbjct: 1115 HYLCKV--LAEKLPELLDFSKDLVNLEAATKVILLLLLCVCIQLKYLAEEMQAVSKGLEKVMQELTASENDGLVSESFC 1191          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: U5FI54_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0018s02490g PE=4 SV=1)

HSP 1 Score: 728.783 bits (1880), Expect = 0.000e+0
Identity = 379/617 (61.43%), Postives = 455/617 (73.74%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDY----TDVANML-------QENLETGCPKSREIASVLHDD---KVEEKSNSEAPEHVTTSPTSDALEKQSICSFQ-LSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESED 2123
            M LFRK F+RKPPDGLLEISERVYVFDCC+  D  EE+EY+VYI  IV KLR+H  DASFMVFNF+EGE +SQI S+LSE+ MTVMDYPR YE  PLL+ E+IHH L+SSE+W SLGQQN++LMH ERGG PVLAFML ALLLY KQ++GEQ+TL+MIYKQ P+ELLHL S +NPLPSQLRYLQYVSRRN+ ++WPP D+ALTLDCIILR IP MD EGGC+PIFRI+GQDPFM  DRTPKVLFSTPK+S+FVQ+YKQADCELVKI+I CH+ GDVV+ECI+LDS+ ERE M+FR+MFNT+FI SNILMLNRDEID LW+ KDQFPKDF AEV+FS++D +    +ID PG+EEK+G+ ++ F +V EIFS +D+    TDVA  +       QENL++  P+  E  SVL +    +V+EK      E+ T SPTS  L KQS  SF+  SD NS R + EP+E++V    A Q  PS   S D++S   K E QEL+VA                           QST +  P LD  S +KK EPQELQVA+Q PAQ KIISQR PQ S+ SPVSY N+L G P  +SRYHS  SALGITALLHDH  S+ E+
Sbjct:    1 MALFRKFFYRKPPDGLLEISERVYVFDCCYCMDTFEEEEYKVYIRGIVGKLRNHLPDASFMVFNFQEGENESQIGSVLSEFDMTVMDYPRHYESFPLLSMEMIHHSLRSSESWLSLGQQNVVLMHCERGGWPVLAFMLAALLLYGKQFTGEQRTLDMIYKQGPQELLHLMSPINPLPSQLRYLQYVSRRNMGTQWPPLDRALTLDCIILRVIPCMDTEGGCQPIFRIHGQDPFMVVDRTPKVLFSTPKRSRFVQHYKQADCELVKIDINCHIQGDVVMECINLDSDHEREQMMFRVMFNTSFISSNILMLNRDEIDTLWDAKDQFPKDFRAEVLFSEMDSSTPIGAIDLPGLEEKDGIPVEVFPRVHEIFSNMDWPDTKTDVAQNMHHHIMEPQENLDSS-PQRAEGGSVLMESTFVRVQEKPKLNESENKTPSPTSITLVKQSTLSFKPFSDTNSVREEAEPQELKV----ALQSMPSIKSSPDANSAREKVEPQELKVA--------------------------CQSTLSIKPSLDAISTQKKVEPQELQVALQWPAQSKIISQRVPQRSLCSPVSYGNNLQGLPVPMSRYHSAPSALGITALLHDHAVSKGEE 586          

HSP 2 Score: 244.588 bits (623), Expect = 1.665e-62
Identity = 115/138 (83.33%), Postives = 127/138 (92.03%), Query Frame = 3

Query: 3390 QLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKVK 3803
            Q+++LIKFCPTKEE ELLKGYNGDKE LGKCEQFFLE++KVPR ESKLRVF FKIQF SQVSDLR  LN+INS +EE+R+S KLKRIMQTIL LGNALN GTARGSAVGFRLDSLLKLT+TRARNNK+TLMHYLCKV+
Sbjct:  990 QVDSLIKFCPTKEEMELLKGYNGDKENLGKCEQFFLEMMKVPRAESKLRVFSFKIQFQSQVSDLRGNLNVINSASEEIRSSAKLKRIMQTILSLGNALNHGTARGSAVGFRLDSLLKLTDTRARNNKITLMHYLCKVQ 1127          

BLAST of g.barbadense_cottongen_reftransV1_0093369 vs. ExPASy TrEMBL
Match: W9RED7_9ROSA (Formin-like protein OS=Morus notabilis GN=L484_013215 PE=3 SV=1)

HSP 1 Score: 715.687 bits (1846), Expect = 0.000e+0
Identity = 360/602 (59.80%), Postives = 433/602 (71.93%), Query Frame = 3

Query:  318 MGLFRKLFFRKPPDGLLEISERVYVFDCCFSTDIREEKEYRVYIENIVCKLRDHFTDASFMVFNFREGEYQSQIASILSEYGMTVMDYPRQYEGCPLLTTELIHHFLKSSENWFSLGQQNIILMHSERGGLPVLAFMLTALLLYRKQYSGEQKTLEMIYKQAPRELLHLTSSLNPLPSQLRYLQYVSRRNVDSEWPPSDKALTLDCIILRCIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKKSKFVQYYKQADCELVKINIRCHVVGDVVLECISLDSNLERELMVFRIMFNTAFIRSNILMLNRDEIDILWNTKDQFPKDFNAEVIFSDIDVAPSAISIDFPGIEEKNGLSIDDFAKVQEIFSRVDYTDVANMLQENLETGCPKSREIASVLHDDKVEEKSNSEAPEHVTTSPTSDALEKQSICSFQLSDANSTRTKFEPEEIQVVPQMATQHTPSAVPSLDSSSTEGKTESQELQVAPQEAELTNSSRRNVEIQEIQVSSQQPAQSTSTFTPVLDVNSIRKKTEPQELQVAVQRPAQPKIISQRGPQSSISSPVSYCNSLPGSPAAISRYHSPLSALGITALLHDHDASESED 2123
            M LFRK F+RKPPDGLLEISERVYVFDCCF+TD+ EE EY+V I  +V +LRDH  DASFMVFNFREGE QSQI SILSEY MTVMDYPR YEGCPLLT E++HHFL+SSE+W SLGQQN++LMH ERGG PVLAFML ALL+YRKQ++GEQKTL+MIYKQAPRELL L S LNP+PSQLRYLQYVSRRNV SEWPP D+ALTL CII+R IPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPK+ K V++YKQADC+L+KI+I CH+ GDVVLECI+LDS+LERE M+FR+MFNTAFIRSNIL+LNRDEIDILWN  DQFPKDF AEV+FS++D + S+ISID PGIEEK GL ++ FAKVQE+FS VD+ D             PK+    ++L   K             + SP++   E + +  F+ S          PE+ +V P+ +  +    + S +   T    +  E  +  Q+ +LT  ++   E QE+Q++ Q+PAQS                                KIISQR P++ +S+PVSY NSL GSP  +SR+HS  SALGITALLHDH AS  E+
Sbjct:    1 MALFRKFFYRKPPDGLLEISERVYVFDCCFTTDVWEEDEYKVRIGGMVSQLRDHSPDASFMVFNFREGEKQSQICSILSEYDMTVMDYPRHYEGCPLLTMEMLHHFLRSSESWLSLGQQNVLLMHCERGGWPVLAFMLAALLIYRKQFTGEQKTLDMIYKQAPRELLQLMSPLNPVPSQLRYLQYVSRRNVGSEWPPLDRALTLVCIIMRLIPNMDGEGGCRPIFRIYGQDPFMAADRTPKVLFSTPKRIKLVRHYKQADCDLLKIDIHCHIQGDVVLECITLDSDLEREEMMFRVMFNTAFIRSNILILNRDEIDILWNANDQFPKDFRAEVLFSEMDASTSSISIDLPGIEEKEGLPMEAFAKVQEMFSDVDWLD-------------PKADAALNLLQQGK-------------SDSPSARIAEMRRL--FRESS---------PEQFKVEPKASESNVSKKMQSQELQDTNSVVKKNETLLV-QDNKLT--TKNKTEPQELQIALQRPAQS--------------------------------KIISQRVPKTPLSAPVSYSNSLQGSP--VSRFHSAPSALGITALLHDHAASNREE 528          

HSP 2 Score: 330.102 bits (845), Expect = 1.593e-89
Identity = 207/311 (66.56%), Postives = 230/311 (73.95%), Query Frame = 3

Query: 2898 GLSKAGDTSPQ-LHSVSXXXXXXXXXXXXXXXLNLKGRGISCIGPKSLAQPRKNNLKPYHWLKLTRAMQGSLWAEAQKPEEAFKAPEIDMSELESLFSAAAPNSDNGSRDGKSNRGASGRKPEKVQLIELRRAYNCEIMLTKVKIPLPXXXXXXXXXXXXXXXXXQLENLIKFCPTKEETELLKGYNGDKEKLGKCEQFFLELIKVPRVESKLRVFLFKIQFSSQVSDLRNGLNIINSTAEEVRNSVKLKRIMQTILYLGNALNKGTARGSAVGFRLDSLLKLTETRARNNKMTLMHYLCKV-KDHLPFLL 3824
            G SKA  TS Q   +VSNGN+PSIPGPPS   L+ KGRG+S + P++ AQP+K+NLKPYHWLKLTRAMQGSLWAEAQK +EA  APE DMSELESLFSAA P+SD+GS  GKSNR ASG K EKVQLIELRRAYNCEIMLTKVK+PLPDL SSVLTLD++ LD DQ+ENLIKFCPTKEE +LLKGY+ DKE LGKCEQ                                            +R+SVKLKRIMQTIL LGNALN GTARGSA+GFRLDSL KLT+TRARNNKMTLMHYLCKV  + LP LL
Sbjct:  796 GASKASLTSQQSCSAVSNGNVPSIPGPPSGVLLSSKGRGLSRMTPRNQAQPKKSNLKPYHWLKLTRAMQGSLWAEAQKNDEASNAPEFDMSELESLFSAAVPSSDHGSPGGKSNRRASGPKAEKVQLIELRRAYNCEIMLTKVKVPLPDLMSSVLTLDESALDVDQVENLIKFCPTKEEMDLLKGYSRDKENLGKCEQ--------------------------------------------IRSSVKLKRIMQTILSLGNALNHGTARGSAIGFRLDSLPKLTDTRARNNKMTLMHYLCKVLAEKLPDLL 1062          

HSP 3 Score: 248.44 bits (633), Expect = 1.681e-63
Identity = 126/158 (79.75%), Postives = 137/158 (86.71%), Query Frame = 3

Query: 4185 HYFITV*VLNEKSPELLEFTKDLVTLEASTKIQLKCLAEEMQAISKGLEKVVQELTASENDGPVCETFCWTLKEFLGFAESEVRSLALLYSSVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLELEKTKAQKEAENEKLKVST 4658
            HY   V  L EK P+LL+F K+L +LEASTK+QLK LAEEMQAISKGLEKVVQELTASENDG V   FC TLKEFL  AE+EVRSLA LYS+VGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLE E+ KAQKEAENEK+K+ +
Sbjct: 1048 HYLCKV--LAEKLPDLLDFPKELASLEASTKVQLKYLAEEMQAISKGLEKVVQELTASENDGRVSGIFCKTLKEFLTDAEAEVRSLASLYSNVGRNADALALYFGEDPARCPFEQVVSTLLNFVRMFVRAHEENCKQLEFERKKAQKEAENEKMKLGS 1203