g.barbadense_cottongen_reftransV1_0092164, g.barbadense_cottongen_reftransV1_0092164 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy Swiss-Prot
Match: M310_ARATH (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 4.547e-29
Identity = 54/143 (37.76%), Postives = 86/143 (60.14%), Query Frame = 3

Query: 1647 AIPTYAMSCFFLRNSLCGEIEMIFAKFWWQKGYGKRGIHWCQWKHMCHPKEEYG-MGLRNMSQFNIALLAKQG*RLITNPDSLVTRVFKAKYFPNEDFLNSCLGNKNSYVWKNIWATKGILAKGLCWKVGTGLNFSIANDAWI 2072
            A+P YAMSCF L   LC ++     +FWW     KR I W  W+ +C  KE+ G +G R++  FN ALLAKQ  R+I  P +L++R+ +++YFP+   +   +G + SY W++I   + +L++GL   +G G++  +  D WI
Sbjct:    2 ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDGIHTKVWLDRWI 144          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy Swiss-Prot
Match: LIN1_NYCCO (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 6.022e-21
Identity = 52/200 (26.00%), Postives = 101/200 (50.50%), Query Frame = 3

Query: 1005 TVE*GFLQAIMLQMGFTRDWVALVMKCITTTSYAVNINGSRARIF*PTRGLRQGDPLSPFLFLMCSEGLSLLMRLAMGEGLLKGIKTCRRGLEISHLFADDCILFDEATTRKAMMIKEILKEYKSCSGQCGNFEKSTVF-YSSISKGD--MKNEIS--------EFLGVRVSTNLEKYFGLPSMVGRRKESFLNLKERIS 1571
             ++  F+   + ++G    ++ L+    +  +  + +NG + + F    G RQG PLSP LF +  E L++ +R    E  +KGI      +++S LFADD I++ E T      + E++KEY + SG   N  KS  F Y++ ++ +  +K+ I         ++LGV ++ +++  +         KE++  L++ I+
Sbjct:  606 NIQHPFMIRTLKKIGIEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSGTRQGCPLSPLLFNIVMEVLAIAIR---EEKAIKGIHIGSEEIKLS-LFADDMIVYLENTRDSTTKLLEVIKEYSNVSGYKINTHKSVAFIYTNNNQAEKTVKDSIPFTVVPKKMKYLGVYLTKDVKDLY---------KENYETLRKEIA 792          

HSP 2 Score: 40.817 bits (94), Expect = 6.022e-21
Identity = 24/61 (39.34%), Postives = 32/61 (52.46%), Query Frame = 1

Query:  712 FKQTYIALIPK-SPNPRTLVNFRPISLCTVIYKFVAKAITNCLQDVIG*CIYQAQSAFILG 891
            F +  I LIPK   +P    N+RPISL  +  K + K +TN +Q  I   I+  Q  FI G
Sbjct:  508 FYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQHIKKIIHHDQVGFIPG 568          

HSP 3 Score: 37.3502 bits (85), Expect = 6.022e-21
Identity = 13/36 (36.11%), Postives = 24/36 (66.67%), Query Frame = 2

Query:  533 LSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRY 640
            L+ P ++ EI + +Q +   K+PGPDGF + F++ +
Sbjct:  448 LNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQTF 483          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy Swiss-Prot
Match: RNHX1_ARATH (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 2.105e-16
Identity = 54/186 (29.03%), Postives = 90/186 (48.39%), Query Frame = 3

Query: 1536 KESFLNLKERISWKFEGRSGRLLSQGVKDIFIKSVLQAIPTYAMSCFFLRNSLCGEIEMIFAKFWWQKGYGKRGIHWCQWKHMCHPKEEYGMGLRNMSQFNIALLAKQG*RLITNPDSLVTRVFKAKYFPNEDFLNSCLGNKNSYVWKNIWAT-----KGILAKGLCWKVGTGLNFSIANDAWIPG 2078
            K++F  + ER+S +  G   + LS   +    K+VL ++P ++MS   L  S+   ++ +   F W     K+  H  +W  +C PK+E G+G+R     N AL++K G RL+   +SL T V + KY   E   +  L  K S  W + W +     + +++ G+ W  G G       D W+ G
Sbjct:   10 KDTFGEILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQKKYHVGEIRDSRWLIPKGS--WSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVSG 193          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy Swiss-Prot
Match: M1250_ARATH (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.923e-12
Identity = 35/66 (53.03%), Postives = 45/66 (68.18%), Query Frame = 3

Query: 1113 INGSRARIF*PTRGLRQGDPLSPFLFLMCSEGLSLLMRLAMGEGLLKGIKTCRRGLEISH-LFADD 1307
            ING+   +  P+RGLRQGDPLSP+LF++C+E LS L R A  +G L GI+       I+H LFADD
Sbjct:   14 INGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRINHLLFADD 79          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy Swiss-Prot
Match: YTX2_XENLA (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis PE=3 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 1.220e-10
Identity = 29/79 (36.71%), Postives = 49/79 (62.03%), Query Frame = 1

Query:  715 KQTYIALIPKSPNPRTLVNFRPISLCTVIYKFVAKAITNCLQDVIG*CIYQAQSAFILGR-LFRIMFF*LMKSYIVFAR 948
            ++  ++L+PK  + R + N+RP+SL +  YK VAKAI+  L+ V+   I+  QS  + GR +F  +F  L++  + FAR
Sbjct:  506 RRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRTIFDNVF--LIRDLLHFAR 582          

HSP 2 Score: 41.2022 bits (95), Expect = 1.220e-10
Identity = 26/83 (31.33%), Postives = 37/83 (44.58%), Query Frame = 2

Query:  416 ISEAATIFFQRLFTSNIIG-DLSHLLMGIKKSFSYELNTKLSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRYWHIAGKD 661
            I + A  F+Q LF+ + I  D    L       S     +L +P    E+  AL+ M   K+PG DG    FF+ +W   G D
Sbjct:  405 IRDRARSFYQNLFSPDPISPDACEELWDGLPVVSERRKERLETPITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPD 487          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy Swiss-Prot
Match: LORF2_MOUSE (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus GN=Pol PE=1 SV=2)

HSP 1 Score: 41.9726 bits (97), Expect = 8.609e-9
Identity = 23/80 (28.75%), Postives = 44/80 (55.00%), Query Frame = 2

Query:  413 EISEAATIFFQRLFTSNIIGDLSHLLMGIKKSFSYELNTK----LSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRY 640
            EI      F++RL+++ +  +L  +   + +    +LN      L+SP + KEI+  +  + + K+PGPDGF A F++ +
Sbjct:  413 EIQNTIRSFYKRLYSTKL-ENLDEMDKFLDRYQVPKLNQDQVDHLNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQTF 491          

HSP 2 Score: 41.2022 bits (95), Expect = 8.609e-9
Identity = 24/61 (39.34%), Postives = 33/61 (54.10%), Query Frame = 1

Query:  712 FKQTYIALIPK-SPNPRTLVNFRPISLCTVIYKFVAKAITNCLQDVIG*CIYQAQSAFILG 891
            F +  I LIPK   +P  + NFRPISL  +  K + K + N +Q+ I   I+  Q  FI G
Sbjct:  516 FYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQVGFIPG 576          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy TrEMBL
Match: F4NCI7_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris PE=4 SV=1)

HSP 1 Score: 321.242 bits (822), Expect = 1.263e-103
Identity = 175/445 (39.33%), Postives = 257/445 (57.75%), Query Frame = 3

Query:  879 FHSREII*DNVLLAYEILHSFCQKRTGNRDIWQ*NLILGKLMT-VE*GFLQAIMLQMGFTRDWVALVMKCITTTSYAVNINGSRARIF*PTRGLRQGDPLSPFLFLMCSEGLSLLMRLAMGEGLLKGIKTCRRGLEISHLF-ADDCILFDEATTRKAMMIKEILKEYKSCSGQCGNFEKSTVFYSSISKGDMKNEISEFLGVRVSTNLEKYFGLPSMVGRRKE-SFLNLKERISWKFEGRSGRLLSQGVKDIFIKSVLQAIPTYAMSCFFLRNSLCGEIEMIFAKFWWQKGYGKRGIHWCQWKHMCHPKEEYGMGLRNMSQFNIALLAKQG*RLITNPDSLVTRVFKAKYFPNEDFLNSCLGNKNSYVWKNIWATKGILAKGLCWKVGTGLNFSIANDAWIPG-AINFRLSSVPFTMTDFKVADLINSAERKWKGELIVDTFGEE 2201
            F  R +I DN L+A+EI H+  +K      +    L + K    VE  FL+ +M +MGF   W+  VM CI++ S+  N+NG       P+RGLRQGDP+SP+LFL+C++  S L+  A  E  + G + CR    +SHLF ADD ILF +A+ ++  M+ +I+ +Y+  SGQ  N  K+ V +S     + ++ I   LGV+     EKY GLP+++GR K+ +F  +KERI  K +G   +LLS+  K++ IKSV QAIPTY MS F L + L  EI  + A+FWW      R +HW  W  +C+PK   G+G R++  FN +LLAKQ  RL T   +L+ R+ +A+YF + + L +  G   S+ W++IW +K +L +GL W VG+G    +  DAWI G   +   +    +  D KV DLI+ A   W  E +  TF EE
Sbjct:  543 FVPRRLITDNALVAFEIFHAMKRKDANKNGVCALKLDMSKAYDRVEWCFLERVMKKMGFCDGWIDRVMACISSVSFTFNVNGVVEGSLSPSRGLRQGDPISPYLFLLCADAFSTLLSKAASEKKIHGAQICRGAPVVSHLFFADDSILFTKASVQECSMVADIISKYERASGQQVNLSKTEVVFSRSVDRERRSAIVNVLGVKEVDRQEKYLGLPTIIGRSKKVTFACIKERIWKKLQGWKEKLLSRPGKEVLIKSVAQAIPTYMMSVFSLPSGLIDEIHSLLARFWWGSSDTNRKMHWHSWDTLCYPKSMGGLGFRDLHCFNQSLLAKQAWRLCTGDQTLLYRLLQARYFKSSELLEARRGYNPSFTWRSIWGSKSLLLEGLKWCVGSGERIRVWEDAWILGEGAHMVPTPQADSNLDLKVCDLIDVARGAWNIESVQQTFVEE 987          

HSP 2 Score: 66.2402 bits (160), Expect = 1.263e-103
Identity = 38/116 (32.76%), Postives = 58/116 (50.00%), Query Frame = 2

Query:  413 EISEAATIFFQRLFTSNIIGDLSHLLMGIKKSFSYELNTKLSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRYWHIAGKDVKNFCLEFQILTRNVNFSNKRTLRLFQKVQIPE 760
            EI      +F+ LF ++   ++   L G+    S ++NT L    +  E+K AL  M   KAPG DG  ALFF+++WHI G DV +F   +     ++   NK  + L  K   P+
Sbjct:  387 EICGVVQHYFEGLFATDSPVNMELALEGLSHCVSTDMNTALLMLPSGDEVKEALFAMHPNKAPGIDGLHALFFQKFWHILGSDVISFVQSWWRGMGDLGVVNKTCIVLIPKCDHPQ 502          

HSP 3 Score: 32.7278 bits (73), Expect = 1.263e-103
Identity = 17/37 (45.95%), Postives = 21/37 (56.76%), Query Frame = 1

Query:  295 Y*EQMARVNWLKFGYKNFVFFHKFTSARKRRNIISML 405
            Y    AR N ++ G KN  +FH   S RKRRN I+ L
Sbjct:  338 YWHARARANEIRDGDKNTKYFHHKASQRKRRNTINEL 374          

HSP 4 Score: 30.0314 bits (66), Expect = 1.263e-103
Identity = 19/50 (38.00%), Postives = 26/50 (52.00%), Query Frame = 2

Query: 2210 ILRIPLAREPHDDFMVCSGEPSGEFSVRSAYKLLQTSDPRAYALQNIYRD 2359
            +L IPL+R   DD        +G FSVRS Y L +    R + LQ+  R+
Sbjct:  992 VLSIPLSRFLPDDHRYWWPSRNGIFSVRSCYWLGRLGPVRTWQLQHGERE 1041          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy TrEMBL
Match: Q2QWF4_ORYSJ (Retrotransposon protein, putative, unclassified OS=Oryza sativa subsp. japonica GN=LOC_Os12g09530 PE=4 SV=1)

HSP 1 Score: 301.982 bits (772), Expect = 1.887e-103
Identity = 166/437 (37.99%), Postives = 249/437 (56.98%), Query Frame = 3

Query:  894 II*DNVLLAYEILHSFCQKRTGNRDIWQ*NLILGKLMT-VE*GFLQAIMLQMGFTRDWVALVMKCITTTSYAVNINGSRARIF*PTRGLRQGDPLSPFLFLMCSEGLSLLMRLAMGEGLLKGIKTCRRGLEISHL-FADDCILFDEATTRKAMMIKEILKEYKSCSGQCGNFEKSTVFYSSISKGDMKNEISEFLGVRVSTNLEKYFGLPSMVGRRK-ESFLNLKERISWKFEGRSGRLLSQGVKDIFIKSVLQAIPTYAMSCFFLRNSLCGEIEMIFAKFWWQKGYGKRGIHWCQWKHMCHPKEEYGMGLRNMSQFNIALLAKQG*RLITNPDSLVTRVFKAKYFPNEDFLNSCLGNKNSYVWKNIWATKGILAKGLCWKVGTGLNFSIANDAWIPGAINFRLSSVPFTMTDFK-VADLINSAERKWKGELIVDTF 2192
            +I DN L+A+E  H+  Q +  +++     L L K    V+  +L+ ++ +MGF+  W+  +M C+T+  YAV  NGS  + F P+RGLRQGDPLSP+LFL  ++GLS ++  A+  G L  +K CR+   ISHL FADD +LF +A   +A ++KE L  Y+ C+ Q  +  K ++ +        +  I   L V+ ++  +KY GLP+  GR K + F ++KER   +    + + L  G K++ IKSV+QA+PTY MS F L  S C E   +  KFWW +   +R +HW  W+ +  PK   GMG ++M  FN ALLA+Q  RL+  PDSL  RV KAKY+PN D L++   + +S  WK I     +L KG+ W++GTG    I  D W+P     ++     T T  K V+DL+   E+ W  ++I   F
Sbjct:  662 MITDNALIAFECFHNIAQSKRESQEFCAYKLDLAKAYDRVDWQYLEGVLDRMGFSNTWIQWMMACVTSVRYAVRFNGSMLKSFQPSRGLRQGDPLSPYLFLFVADGLSTILSSAVRTGDLVPLKVCRQAPGISHLLFADDSLLFFKANVEQATVVKEALNLYERCTSQLLSPAKCSMLFGKHCSDQTQPGIKSLLDVQQNSFEDKYLGLPTPEGRMKADRFQSIKERFFKRLIDWAEKHLPMGGKEVLIKSVIQALPTYMMSVFKLPPSFCEEYMQLVRKFWWGEDRSQRKVHWASWQQLIKPKSMGGMGFKDMKIFNQALLARQAWRLVQFPDSLCARVLKAKYYPNGDLLDTAFSSVSSDSWKGIVHGLELLKKGVIWRIGTGRKVHIWRDDWLPREHGLKVMGKK-TGTRLKWVSDLLVPNEQSWDEQVIRHIF 1097          

HSP 2 Score: 63.929 bits (154), Expect = 1.887e-103
Identity = 36/70 (51.43%), Postives = 43/70 (61.43%), Query Frame = 1

Query:  694 DQKC--EFFKQTYIALIPKSPNPRTLVNFRPISLCTVIYKFVAKAITNCLQDVIG*CIYQAQSAFILGRL 897
            DQK   E    T I LIPK    + L +FRPISLC VIYK VAK + N L+ ++   I Q QSAFI GR+
Sbjct:  593 DQKVMPEGVNTTAIVLIPKKKESKELKDFRPISLCNVIYKVVAKCLVNHLRPILESIISQEQSAFIPGRM 662          

HSP 3 Score: 62.003 bits (149), Expect = 1.887e-103
Identity = 34/84 (40.48%), Postives = 46/84 (54.76%), Query Frame = 2

Query:  413 EISEAATIFFQRLFTSNIIGDLSHLLMGIKKSFSYELNTKLSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRYWHIAGKDV 664
            E+   AT FF+ L+T + +     LL  I    + ++N  L   F+ KEI  AL  +   KAPGPDGFPA FF+R W +   DV
Sbjct:  501 EMETMATNFFKDLYTKDPLVTPQPLLDLIMLKVTEQMNEDLCKAFSDKEISDALFQIGPIKAPGPDGFPARFFQRNWEVLKNDV 584          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy TrEMBL
Match: B9EYH0_ORYSJ (Uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_02920 PE=4 SV=1)

HSP 1 Score: 277.715 bits (709), Expect = 3.336e-103
Identity = 152/441 (34.47%), Postives = 244/441 (55.33%), Query Frame = 3

Query:  894 II*DNVLLAYEILHSFCQKRTGNRDIWQ*NLILGKLMTVE*GFLQAIMLQMGFTRDWVALVMKCITTTSYAVNINGSRARIF*PTRGLRQGDPLSPFLFLMCSEGLSLLMRLAMGEGLLKGIKTCRRGLEISH-LFADDCILFDEATTRKAMMIKEILKEYKSCSGQCGNFEKSTVFYSSISKGDMKNEISEFLGVRVSTNLEKYFGLPSMVGRRKESFLN-LKERISWKFEGRSGRLLSQGVKDIFIKSVLQAIPTYAMSCFFLRNSLCGEIEMIFAKFWWQKGYGKRGIHWCQWKHMCHPKEEYGMGLRNMSQFNIALLAKQG*RLITNPDSLVTRVFKAKYFPNEDFLNSCLGNKNSYVWKNIWATKGILAKGLCWKVGTGLNFSIANDAWIPGAINFRLSSVPFTMTDFKVAD-LINSAERKWKGELIVDTFGEEVA 2207
            +I D+ L+A+E LHS  ++ + N        ++     +E  +LQ  + ++GF   WV+ VM+C+T+  YAV ING   R+  P+RG+RQGDP+SP+LFL+C+EGLS L++     GLL GI+   +G  ISH LFADD I F ++  R   ++K  L+ Y + SGQ  N +KS++ +       +K  + + L V      + Y G+P+ +     S  N L ER+  +  G + R LS+  K+  +K+V+QAIPTY MSCF +  ++C +++   A  WW    G++ +HW                  ++  FN A+LA+QG R++T+P+SL +RV K +YFP+ DF ++      S+ W++I   + +L KG+ W VG G    I  D WIPG +   + +  F + D    D L+N     W  E++   F +++A
Sbjct:  285 LITDSALVAFECLHSIRRQHSKNPFFALKIDMMKAYDRIEWSYLQGCLTKLGFAPAWVSSVMRCVTSVRYAVRINGELTRLVIPSRGIRQGDPVSPYLFLLCTEGLSCLLQRKESLGLLHGIRNGHQGPPISHLLFADDSIFFAKSDPRSVQVLKSTLQSYCNASGQKINLQKSSLLFGRYCPDAIKTCVKDCLQVSNEALQDTYLGMPTEINCAVTSSFNFLSERVWKRVNGWNDRPLSRAGKETMLKAVVQAIPTYIMSCFQVPTTICDKMKSSMANHWWGYEDGRKKMHW----------------RSDLVIFNQAMLARQGWRILTDPNSLCSRVLKGRYFPDSDFWSAPKPRSASFTWRSILFGRELLKKGVRWAVGDGSTIKITKDKWIPG-VQAGMVNTRFPIPDDVTVDWLMNPERNAWDEEIVKAVFNDDIA 708          

HSP 2 Score: 63.929 bits (154), Expect = 3.336e-103
Identity = 31/79 (39.24%), Postives = 45/79 (56.96%), Query Frame = 2

Query:  428 ATIFFQRLFTSNIIGDLSHLLMGIKKSFSYELNTKLSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRYWHIAGKDV 664
            A  FF++LFTS     +  +L  I       +N +L   ++ +EIK AL  M  TKAP PDGFPALF++ +W+    D+
Sbjct:  129 AEHFFKKLFTSEQCESVEEVLEAIPCKVDESINEELCKAYSDEEIKEALFQMGPTKAPSPDGFPALFYQSHWNFLENDI 207          

HSP 3 Score: 51.6026 bits (122), Expect = 3.336e-103
Identity = 30/73 (41.10%), Postives = 40/73 (54.79%), Query Frame = 1

Query:  679 GISNSDQKCEFFKQTYIALIPKSPNPRTLVNFRPISLCTVIYKFVAKAITNCLQDVIG*CIYQAQSAFILGRL 897
            G    D+  E    + I LIPK  N   L  FRPISLC V+YK  +K + N L+  +   + + QSAF+ GRL
Sbjct:  213 GFLTGDEIPEGLCDSIIVLIPKIHNLVHLSKFRPISLCNVLYKIASKVLANRLKIFLPDIVSEFQSAFVPGRL 285          

HSP 4 Score: 42.743 bits (99), Expect = 3.336e-103
Identity = 22/58 (37.93%), Postives = 35/58 (60.34%), Query Frame = 1

Query:  244 IIFLKRLLYMEI----DKHKMY*EQMARVNWLKFGYKNFVFFHKFTSARKRRNIISML 405
            II  +RLL  ++    ++ ++  +Q +R+ WLK G +N  FFH   SAR+R N I +L
Sbjct:   54 IIREERLLEQQLCDLFEREEIMAKQRSRIEWLKEGDRNTSFFHARASARRRTNKIKLL 111          

HSP 5 Score: 33.113 bits (74), Expect = 3.336e-103
Identity = 13/40 (32.50%), Postives = 27/40 (67.50%), Query Frame = 2

Query: 2201 SSRILRIPLAREPHDDFMVCSGEPSGEFSVRSAYKLLQTS 2320
            +  +L++P++R   +DF+    +  G F+VRSAY L++++
Sbjct:  708 AREVLQVPISRHGGEDFVSWPHDKRGLFTVRSAYNLVRSN 747          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy TrEMBL
Match: Q2HS45_MEDTR (RNA-directed DNA polymerase (Reverse transcriptase) OS=Medicago truncatula GN=MtrDRAFT_AC157503g30v2 PE=4 SV=2)

HSP 1 Score: 301.597 bits (771), Expect = 5.019e-103
Identity = 170/443 (38.37%), Postives = 247/443 (55.76%), Query Frame = 3

Query:  879 FHSREII*DNVLLAYEILHSFCQKRTGNRDIWQ*NLILGKLMT-VE*GFLQAIMLQMGFTRDWVALVMKCITTTSYAVNINGSRARIF*PTRGLRQGDPLSPFLFLMCSEGLSLLMRLAMGEGLLKGIKTCRRGLEISHL-FADDCILFDEATTRKAMMIKEILKEYKSCSGQCGNFEKSTVFYSSISKGDMKNEISEFLGVRVSTNLEKYFGLPSMVGRRKESFLN-LKERISWKFEGRSGRLLSQGVKDIFIKSVLQAIPTYAMSCFFLRNSLCGEIEMIFAKFWW-QKGYGKRGIHWCQWKHMCHPKEEYGMGLRNMSQFNIALLAKQG*RLITNPDSLVTRVFKAKYFPNEDFLNSCLGNKNSYVWKNIWATKGILAKGLCWKVGTGLNFSIANDAWIPGAINFRLSSVPFTMTDFKVADLINSAERKWKGELIVDTFG 2195
            F +   I DN+L+A EI+H    K  G +      + + K    V+  +L +IM +MGF   W+  +   +T  SY V +N  R     P RGLRQGDPLSP+LF++C+EG+S L++ A    +L G+K C+R   +SHL FADD  LF  A   +   +K+IL  Y + S Q  N +KS +++S       KN +S  L V     + KY GLPSM+GR K+S  N +K+RI  +  G S ++LSQ  K++ IKSV QAIP+Y MS F L +S+  +IE +   FWW  K  G+ GI W  W+ +   KE  GMG R++  F++A+  KQ    +TNPD++V+R+FKAKYF NE+FL + LG+  SYVW++IW+++  L  G  W++G G                          T   V+ LI++  R W   LI + F 
Sbjct:  132 FVAGRTITDNILIALEIIHYLKCKTWGIKGEAALKIDISKTYDRVDWNYLFSIMEKMGFNPIWIKWMRMSVTMVSYHVLVNDDRVGPIYPGRGLRQGDPLSPYLFILCAEGMSTLIKQAERNNILHGVKVCKRAPTVSHLLFADDSFLFFRANENETRALKDILDTYANASDQLINMQKSEIYFSRNVPVTKKNTLSNMLWVSEGIGIRKYLGLPSMIGRSKKSIFNYIKDRIWNRISGLSSKMLSQAGKEVLIKSVAQAIPSYCMSVFLLPHSIADDIEKMLNSFWWGMKNEGRGGIRWMSWERLTMRKEWGGMGFRHIHGFDLAMSGKQCWNFLTNPDAMVSRIFKAKYFTNENFLGASLGHNPSYVWRSIWSSRIFLKTGYQWRIGDG-------------------------STTATVSHLIDAQTRSWNEPLIRNNFN 549          

HSP 2 Score: 65.4698 bits (158), Expect = 5.019e-103
Identity = 31/59 (52.54%), Postives = 39/59 (66.10%), Query Frame = 1

Query:  718 QTYIALIPKSPNPRTLVNFRPISLCTVIYKFVAKAITNCLQDVIG*CIYQAQSAFILGR 894
             T I LIPK  +PRT+ + RPISLC V+YK +AK + N LQ V+  CI   QS F+ GR
Sbjct:   78 DTNIVLIPKCDHPRTMRDLRPISLCNVVYKILAKTLANRLQRVLDKCISVEQSGFVAGR 136          

HSP 3 Score: 53.5286 bits (127), Expect = 5.019e-103
Identity = 22/66 (33.33%), Postives = 38/66 (57.58%), Query Frame = 2

Query:  488 LMGIKKSFSYELNTKLSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRYWHIAGKDVKNFCLEF 685
            +  + +  + E N  L +PF  +E + A+  M+S KAPGPDG    F++R+W + G++V   C  +
Sbjct:    1 MQAVAECLNEEDNQFLIAPFTDEEFRDAIFQMQSDKAPGPDGLNPTFYKRFWTLCGEEVVAACRRW 66          

HSP 4 Score: 27.335 bits (59), Expect = 5.019e-103
Identity = 11/33 (33.33%), Postives = 21/33 (63.64%), Query Frame = 2

Query: 2210 ILRIPLAREPHDDFMVCSGEPSGEFSVRSAYKL 2308
            IL+IPL     +D ++   +  G +SV++AY++
Sbjct:  556 ILKIPLLHCEQNDAIIWRFDKKGVYSVKNAYRV 588          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy TrEMBL
Match: M5XSK0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016553mg PE=4 SV=1)

HSP 1 Score: 345.51 bits (885), Expect = 1.681e-102
Identity = 189/446 (42.38%), Postives = 269/446 (60.31%), Query Frame = 3

Query:  885 SREII*DNVLLAYEILHSFCQKRTGNRDIWQ*NLILGKLMT-VE*GFLQAIMLQMGFTRDWVALVMKCITTTSYAVNINGSRARIF*PTRGLRQGDPLSPFLFLMCSEGLSLLMRLAMGEGLLKGIKTCRRGLEISHLF-ADDCILFDEATTRKAMMIKEILKEYKSCSGQCGNFEKSTV-FYSSISKGDMKNEISEFLGVRVSTNLEKYFGLPSMVGRRK-ESFLNLKERISWKFEGRSGRLLSQGVKDIFIKSVLQAIPTYAMSCFFLRNSLCGEIEMIFAKFWWQKGYGKRGIHWCQWKHMCHPKEEYGMGLRNMSQFNIALLAKQG*RLITNPDSLVTRVFKAKYFPNEDFLNSCLGNKNSYVWKNIWATKGILAKGLCWKVGTGLNFSIANDAWIPGAINFRL-SSVPFTMTDFKVADLINSAERKWKGELIVDT-FGEEV 2204
            S  +I DN +LA EI+H   ++R G +      + + K    +E  FL+AIM ++GF   W+ L++ CI+T SY+  ING+      P+RGLRQGDPLSP+LFL+C+EGL+ L+     EG LKG+  CR    ISHLF ADD  LF  A     M +K+IL  Y+   GQ  NF+KS V F  ++ +GD +  +++F+G+    +  +Y GLP ++ ++K  SF +LKER+  K +   G+LLS   K+I IK V QAIP Y MSCF L   +C ++  + A+FWW      + IHW  W  +C PKEE G+G RN+  FN+ALLAKQG RL+ NPDSLVT+V KAKYFP   FL + +    S VWK++   + ++ +G  W+VG+G    I  D W+P   +F++ S  P      KV+DLI+   R+W   L+ +  F EEV
Sbjct:  187 SGRLISDNTILAAEIIHYLRRRRRGKKGFMALKMDMSKAYDRIEWSFLEAIMRKLGFAEQWIQLMLTCISTVSYSFVINGTPHGFLHPSRGLRQGDPLSPYLFLLCAEGLTALIAQKEREGFLKGVSICRGAPAISHLFFADDSFLFAWANMADCMALKDILDTYERALGQQVNFQKSAVCFSKNVHRGD-QLMLAQFMGIPCVDHHSQYLGLPMVLDKKKGASFNHLKERLWKKLQTWKGKLLSGAGKEILIKVVAQAIPIYTMSCFLLPKYVCEDLNKLVAQFWWNSSTENKKIHWMAWDRLCAPKEEGGLGFRNLHAFNLALLAKQGWRLLQNPDSLVTKVLKAKYFPTRSFLETTVSPYASVVWKSLCDARTVIIQGSRWQVGSGDTIGIWEDRWLPQPNSFQIFSPRPEHSAITKVSDLIHGDSREWNAPLLQNVFFPEEV 631          

HSP 2 Score: 54.299 bits (129), Expect = 1.681e-102
Identity = 28/67 (41.79%), Postives = 40/67 (59.70%), Query Frame = 1

Query:  697 QKCEFFKQTYIALIPKSPNPRTLVNFRPISLCTVIYKFVAKAITNCLQDVIG*CIYQAQSAFILGRL 897
            QK  F   T++ LIPK  +P+ +   RPISLC V++K   K + N L+ ++   I  +QSA I GRL
Sbjct:  127 QKINF---THVVLIPKVKHPKDMSQLRPISLCNVLFKIATKVLANRLKLILDKIISPSQSALISGRL 190          

HSP 3 Score: 25.409 bits (54), Expect = 1.681e-102
Identity = 16/44 (36.36%), Postives = 23/44 (52.27%), Query Frame = 2

Query: 2210 ILRIPLAREPHDDFMVCSGEPSGEFSVRSAYKL---LQTSDPRA 2332
            I  IPL+     D +V   +  G F+V+SAY +   L +S  RA
Sbjct:  634 IRSIPLSLRLTPDMLVWHYDKKGMFTVKSAYHVARSLHSSTGRA 677          

HSP 4 Score: 70.4774 bits (171), Expect = 3.520e-9
Identity = 39/112 (34.82%), Postives = 64/112 (57.14%), Query Frame = 2

Query:  437 FFQRLFTSNIIGDLSHLLMGIKKSFSYELNTKLSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRYWHIAGKDV----KNFCLEFQILTRNVNFSNKRTLRLFQKVQIPE 760
            +F  LFTS+  G    ++  ++   + ++N  L + +   EI  A+  M  TKAPGPDG P +FF++YWHI G DV    K+F    ++L + +NF++   + L  KV+ P+
Sbjct:   37 YFTHLFTSSASGSEGEIIDAVESRVTPDMNNLLLTDYCDAEIHEAVFQMYPTKAPGPDGMPPIFFQKYWHIVGSDVTRAIKDFLTSGRLLQK-INFTH---VVLIPKVKHPK 144          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy TrEMBL
Match: M5XK32_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa016563mg PE=4 SV=1)

HSP 1 Score: 297.36 bits (760), Expect = 5.288e-102
Identity = 168/443 (37.92%), Postives = 243/443 (54.85%), Query Frame = 3

Query:  876 CFHSREII*DNVLLAYEILHSFCQKRTGNRDIWQ*NLILGKLMT-VE*GFLQAIMLQMGFTRDWVALVMKCITTTSYAVNINGSRARIF*PTRGLRQGDPLSPFLFLMCSEGLSLLMRLAMGEGLLKGIKTCRRGLEISHLFADDCILFDEATTRKAMMIKEILKEYKSCSGQCGNFEKSTVFYSSISKGDMKNEISEFLGVRVSTNLEKYFGLPSMVGRRKE-SFLNLKERISWKFEGRSGRLLSQGVKDIFIKSVLQAIPTYAMSCFFLRNSLCGEIEMIFAKFWWQKGYGKRGIHWCQWKHMCHPKEEYGMGLRNMSQFNIALLAKQG*RLITNPDSLVTRVFKAKYFPNEDFLNSCLGNKNSYVWKNIWATKGILAKGLCWKVGTGLNFSIANDAWIPGAINFRLSSVPFT-MTDFKVADLI-NSAERKWKGELIVDTF 2192
             F    +I DN ++A+EI H   Q+R G +      L + K    VE  FL+ +ML MGF   WV +VM C+TT SY+  +NG   RI  PTRGLRQGDPLSP+LFL+C+E                             +   D  +F +AT     ++K I + Y+  SG+  N +KS V +S+    D ++ ++  LGV    +   Y GLP M+GR K   F  LKER+  K +G   + LS   K++ +K V Q+IP Y M+CF L   LC EIE + A+FWW +    R IHW +W+ +C  K E GMG R +  FN+A+LAKQG RL+ NP SL +R+ KAKYFP+ +F  + LG++ S VWK+IW  + +L  G  +++G G +  I  D W+P    F + + P   M + KV++LI N    +W  + + + F
Sbjct:  138 AFVPGRLITDNSIVAFEIAHFLKQRRRGRKGSLALKLDMSKAYDRVEWEFLEKMMLAMGFPILWVRMVMDCVTTVSYSFLVNGEPTRILYPTRGLRQGDPLSPYLFLLCAE-----------------------------VLPHDSFVFAKATDNNCGVLKHIFEVYERASGEQINCQKSCVAFSANIHMDTQSRLASVLGVPRVDSHATYLGLPMMLGRNKTFCFRYLKERVWKKLQGWREQTLSIAGKEVLLKVVAQSIPLYVMNCFLLPQGLCHEIEQMMARFWWGQQGENRKIHWMRWERLCKAKTEGGMGFRCLQAFNMAMLAKQGWRLVHNPHSLASRLLKAKYFPHTNFWEATLGSRPSCVWKSIWTARKVLEMGSRFQIGDGKSVRIWGDKWVPRPAAFVVITSPLDGMENTKVSELICNEGSPQWDLQKLNNLF 551          

HSP 2 Score: 62.003 bits (149), Expect = 5.288e-102
Identity = 28/60 (46.67%), Postives = 40/60 (66.67%), Query Frame = 1

Query:  718 QTYIALIPKSPNPRTLVNFRPISLCTVIYKFVAKAITNCLQDVIG*CIYQAQSAFILGRL 897
             T++ LIPK   PRT+   RPISLC V+Y+  AK + N ++ V+   I ++QSAF+ GRL
Sbjct:   85 HTFVTLIPKVKEPRTMAQLRPISLCNVLYRIGAKTLANRMKFVMQSVISESQSAFVPGRL 144          

HSP 3 Score: 59.3066 bits (142), Expect = 5.288e-102
Identity = 24/63 (38.10%), Postives = 40/63 (63.49%), Query Frame = 2

Query:  476 LSHLLMGIKKSFSYELNTKLSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRYWHIAGKDV 664
            +  +L  ++   + ++   L + F+ +EIK A+  M+ +KAPGPDG P LF+++YW I G DV
Sbjct:    4 MEEILSALEPKVTADMQQVLIADFSYQEIKDAVFQMQPSKAPGPDGLPPLFYQKYWRIVGDDV 66          

HSP 4 Score: 25.7942 bits (55), Expect = 5.288e-102
Identity = 12/32 (37.50%), Postives = 21/32 (65.62%), Query Frame = 2

Query: 2213 LRIPLAREPHDDFMVCSGEPSGEFSVRSAYKL 2308
            +RIPL+     D +V + +  G F+V+SAY++
Sbjct:  560 VRIPLSIRAPPDRIVWNYDKHGLFTVKSAYRV 591          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy TrEMBL
Match: M5WJW2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020180mg PE=4 SV=1)

HSP 1 Score: 313.923 bits (803), Expect = 2.031e-101
Identity = 180/463 (38.88%), Postives = 273/463 (58.96%), Query Frame = 3

Query:  876 CFHSREII*DNVLLAYEILHSF--CQKRTGNRDIWQ*NLILGKLMT---VE*GFLQAIMLQMGFTRDWVALVMKCITTTSYAVNINGSRARIF*PTRGLRQGDPLSPFLFLMCSEGLSLLMRLAMGEGLLKGIKTCRRGLEISHL-FADDCILFDEATTRKAMMIKEILKEYKSCSGQCGNFEKSTVFYSSISKGDMKNEISEFLGVRVSTNLEKYFGLPSMVGR-RKESFLNLKERISWKFEGRSGRLLSQGVKDIFIKSVLQAIPTYAMSCFFLRNSLCGEIEMIFAKFWWQKGYGKRGIHWCQWKHMCHPKEEYGMGLRNMSQFNIALLAKQG*RLITNPDSLVTRVFKAKYFPNEDFLNSCLGNKNSYVWKNIWATKGILAKGLCWKVGTGLNFSIANDAWIPGAINFRLSSVPFTMTDFKVADLINSAERKWKGELIVDTFGEEVAGSFVSLWRESLT 2243
             F    +I DNV+ A+EI+++    +KR   RD+ Q  L L        VE  FL+A+ML++GF+  WV+ VM CI+TT+++V   G+      P RGLRQG PLSP+LFL+C+EG S L+  A   G L G++  R    ++HL FADD ILF +AT +  M ++ + + Y+  +GQ  N+ KS +  S  +     + I   L V V    E Y GLP++ G+ RK+ F +LK+++     G   +LLS+  K+I IK+VLQAIPTY+MSCF +   LC E+  I A+FWW K   KRGIHW +W+ +C  K   G+G R++  FN ALLAKQ  R++  P+SLV R+F+A+Y P+  FL + +G   S++W ++   K +L KG+ W+VG+G++  +  D W+P    F++ S P      +V DL  S+  +W   L+ D F ++   + + +   SL 
Sbjct:  492 AFVPNRMILDNVMAAFEIMNTIKGVKKR---RDV-QMALKLDMAKAYDRVEWVFLRAMMLKLGFSATWVSKVMDCISTTTFSVLWKGTPVGHIMPQRGLRQGCPLSPYLFLICTEGFSCLLHGAERRGDLVGVQVARGAPSVTHLLFADDSILFMKATNKACMALETLFQTYEEVTGQQINYSKSALSLSPNATRADFDMIEGGLNVPVVRCHENYLGLPTIAGKGRKQLFQHLKDKLWKHISGWKEKLLSRAGKEILIKAVLQAIPTYSMSCFQIPKGLCKELNGIMARFWWAKAKDKRGIHWVKWELLCKSKFAGGLGFRDLEAFNQALLAKQCWRILRTPESLVARIFRARYHPSVPFLEAEVGTNPSFIWGSLQWGKELLNKGVRWRVGSGVSIQVYTDKWLPAPSCFKIMSPPQLPLSTRVCDLFTSSG-QWNVPLLKDIFWDQEVDAILQIPLASLA 949          

HSP 2 Score: 70.0922 bits (170), Expect = 2.031e-101
Identity = 37/107 (34.58%), Postives = 58/107 (54.21%), Query Frame = 2

Query:  437 FFQRLFTSNIIGDLSHLLMGIKKSFSYELNTKLSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRYWHIAGKDVKNFCLEFQILTRNVNFSNKRTLRLFQKVQIP 757
            +F+ LF+S+    +  +L  ++   +  +N +L   F  +E++  L  M  TKAPG DG PALFF++YWHI G  V   CL+      +V   N   + L  KV++P
Sbjct:  345 YFKTLFSSSGGQQMERILNEVRPVITSAMNDRLLQAFTREELEHTLFQMFPTKAPGHDGMPALFFQKYWHIVGDKVAKKCLQILNGEGSVREFNHTLIALIPKVKMP 451          

HSP 3 Score: 37.3502 bits (85), Expect = 2.031e-101
Identity = 17/40 (42.50%), Postives = 25/40 (62.50%), Query Frame = 1

Query:  277 IDKHKMY*EQMARVNWLKFGYKNFVFFHKFTSARKRRNII 396
            ++K ++   Q +RV WLK G KN  FFH   S+R +RN +
Sbjct:  282 LEKQEIMWRQRSRVVWLKEGDKNTHFFHGRASSRSKRNRV 321          

HSP 4 Score: 66.2402 bits (160), Expect = 7.246e-8
Identity = 34/72 (47.22%), Postives = 43/72 (59.72%), Query Frame = 1

Query:  682 ISNSDQKCEFFKQTYIALIPKSPNPRTLVNFRPISLCTVIYKFVAKAITNCLQDVIG*CIYQAQSAFILGRL 897
            I N +     F  T IALIPK   P T+  FRPISLCT +YK +AK I N L+ V+   I + QSAF+  R+
Sbjct:  427 ILNGEGSVREFNHTLIALIPKVKMPTTVSEFRPISLCTTVYKMIAKTIANRLKTVLPHVITETQSAFVPNRM 498          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy TrEMBL
Match: Q7XLZ5_ORYSJ (OSJNBa0086O06.14 protein OS=Oryza sativa subsp. japonica GN=OSJNBa0086O06.14 PE=4 SV=2)

HSP 1 Score: 277.715 bits (709), Expect = 4.280e-100
Identity = 163/460 (35.43%), Postives = 243/460 (52.83%), Query Frame = 3

Query:  894 II*DNVLLAYEILHSFCQKRTGNRDIWQ*NLILGKLMT-VE*GFLQAIMLQMGFTRDWVALVMKCITTTSYAVNINGSRARIF*PTRGLRQGDPLSPFLFLMCSEGLSLLMRLAMGEGLLKGIKTCRRGLEISHL-FADDCILFDEATTRKAMMIKEILKEYKSCSGQCGNFEKSTVFYSSISKGDMKNEISEFLGVRVSTNLEKYFGLPSMVGRRK-ESFLNLKERISWKFEGRSGRLLSQGVKDIFIKSVLQAIPTYAMSCFFLRNSLCGEIEMIFAKFWWQKGYGKRGIHWCQWKHMCHPKEEYGMGLRNMSQFNIALLAKQG*RLITNPDSLVTRVFKAKYFPNEDFLNSCLGNKNSYVWKNIWATKGILAKGLCWKVGTGLNFSIANDAWIPGAINFRLSSVPFTMTDFKVADLINSAERKWKGELIVDTFGEEVAGSFVSL---WRESLTMISW 2255
            +I DN L+A+E  HS  +    ++D     L L K    V+ GFL   + ++GF   W   +M C+T+  Y+V +NG+    F PTRGLR+GDPL+P+LFL  ++GLS +++    E  ++ +K CR    +SHL FADD +LF +A   +A  IKE L  Y+ C+GQ  N ++ ++ +S++   + ++ I   L V  +   +K  GLP+  GR K E F  +KER   +    S R LS   K+  IKSV QA+PTY M  F +    C E E +   FWW    G++ +HW  W+ +  PK   G+G R++  FN ALLA+Q  RLI +PDSL  RV KAKY+PN    ++   + +S  WK I     +L KGL W++G G    I  + W+    N ++           V +LI +  + W   LI     EE A   + +    RE     +W
Sbjct:  533 MITDNALVAFECFHSIHKCTRESQDFCALKLDLSKAYDRVDWGFLDGALQKLGFGNIWRKWIMSCVTSVRYSVRLNGNMLEPFYPTRGLREGDPLNPYLFLFIADGLSNILQRRRDERQIQPLKVCRSAPGVSHLLFADDSLLFFKAEVIQATRIKEALDLYERCTGQLINPKECSLLFSALCPQERQDGIKAVLQVERTCFDDKCLGLPTPDGRMKAEQFQPIKERFEKRLTDWSERFLSLAGKEALIKSVAQALPTYTMGVFKMPERFCEEYEQLVRNFWWGHEKGEKKVHWIAWEKLTSPKLLGGLGFRDIRCFNQALLARQAWRLIESPDSLCARVLKAKYYPNGTITDTAFPSVSSPTWKGIVHGLELLKKGLIWRIGDGSKTKIWRNHWVAHGENLKILEKKTWNRVIYVRELIVTDTKTWNEPLIRHIIREEDADEILKIRIPQREEEDFPAW 992          

HSP 2 Score: 59.6918 bits (143), Expect = 4.280e-100
Identity = 29/64 (45.31%), Postives = 41/64 (64.06%), Query Frame = 1

Query:  706 EFFKQTYIALIPKSPNPRTLVNFRPISLCTVIYKFVAKAITNCLQDVIG*CIYQAQSAFILGRL 897
            E    T I +IPK+  P  + +FRP+SLC VIYK VAK + N L+ ++   I + QSAF+ GR+
Sbjct:  470 EGMNDTVIVMIPKTNAPVEMKDFRPVSLCNVIYKVVAKCLVNRLRPLLQEIISETQSAFVPGRM 533          

HSP 3 Score: 55.8398 bits (133), Expect = 4.280e-100
Identity = 27/56 (48.21%), Postives = 34/56 (60.71%), Query Frame = 2

Query:  518 ELNTKLSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRYWHIAGKDVKNFCLEF 685
            E+N  L+ PF  +EI  AL  +   KAPGPDGFPA FF+R W +  +DV     EF
Sbjct:  407 EMNGMLTKPFTDEEISDALFQIGPLKAPGPDGFPARFFQRNWGVLKRDVIEGVREF 462          

HSP 4 Score: 44.669 bits (104), Expect = 4.280e-100
Identity = 24/74 (32.43%), Postives = 40/74 (54.05%), Query Frame = 1

Query:  184 EMTTSHLVEKDQS*LPVFVFIIFLKRLLYMEIDKHKMY*EQMARVNWLKFGYKNFVFFHKFTSARKRRNIISML 405
            E+    +  ++ S   V    IFL+ LL+ E    +++ +Q +R+ WLK G +N  +FH   S R R+N+I  L
Sbjct:  332 ELEEMRMRGREDSEPDVHRLKIFLQELLHRE----EIWWKQRSRITWLKEGDRNTRYFHLKASWRARKNLIKKL 401          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy TrEMBL
Match: M5WKF7_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa022768mg PE=4 SV=1)

HSP 1 Score: 325.479 bits (833), Expect = 4.844e-99
Identity = 174/412 (42.23%), Postives = 248/412 (60.19%), Query Frame = 3

Query:  876 CFHSREII*DNVLLAYEILHSFCQKRTGNRDIWQ*NLILGKLMT-VE*GFLQAIMLQMGFTRDWVALVMKCITTTSYAVNINGSRARIF*PTRGLRQGDPLSPFLFLMCSEGLSLLMRLAMGEGLLKGIKTCRRGLEISHL-FADDCILFDEATTRKAMMIKEILKEYKSCSGQCGNFEKSTVFYSSISKGDMKNEISEFLGVRVSTNLEKYFGLPSMVGRRKES-FLNLKERISWKFEGRSGRLLSQGVKDIFIKSVLQAIPTYAMSCFFLRNSLCGEIEMIFAKFWWQKGYGKRGIHWCQWKHMCHPKEEYGMGLRNMSQFNIALLAKQG*RLITNPDSLVTRVFKAKYFPNEDFLNSCLGNKNSYVWKNIWATKGILAKGLCWKVGTGLNFSIANDAWIPGAINFRLSS 2102
             F S   I DN L A EI +   ++R G +      L + K    VE  FLQAIM ++GF   W+ LV+ CI++ SY+  ING       P+RGLRQGDPLSP+LFL C+EGLS L+       LLKG+  C+    ISHL FADD + F  AT  +  +I+ IL +Y+  SGQ  N  KS+V +SS  K   +  ++  LGV+ +   ++Y G+P++VGRR+ + F  LKERI  +  G  G+LLS   K+I +K+V+QAIP Y+MS F L      ++  + A FWW    G R IHW  W+ +C PK+E GMG R++  FN+A+LAKQG R++T P S+ +++ +AKYFP   FLN+ + + +S VWK++ A + +L  G  W+V  G N  I +D WIP    F++ S
Sbjct:  120 AFISEWSIVDNTLTAAEIAYFLKKRRRGKKGFMALKLDMSKAYDRVEWHFLQAIMGKLGFNARWINLVLTCISSVSYSFLINGILTGYLHPSRGLRQGDPLSPYLFLFCAEGLSTLIAKKESAWLLKGVTICKEAPSISHLLFADDSLFFALATVEECQVIRAILAQYEQESGQQLNLMKSSVVFSSNIKRPAQLTLAAALGVQQADYHDRYLGMPTLVGRRRSACFSYLKERIMKRLHGWKGKLLSGAGKEILLKAVVQAIPLYSMSYFLLPRGFYDDLNKLMAGFWWNNSNGDRKIHWISWEKLCKPKDEGGMGFRHLYAFNLAMLAKQGWRILTMPTSMASKLLQAKYFPTSSFLNASVSSHDSAVWKSLCAARKVLEGGTRWQVSDGNNIDIWHDRWIPQPRQFKIYS 531          

HSP 2 Score: 62.7734 bits (151), Expect = 4.844e-99
Identity = 40/103 (38.83%), Postives = 56/103 (54.37%), Query Frame = 2

Query:  449 LFTSNIIGDLSHLLMGIKKSFSYELNTKLSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRYWHIAGKDVKNFCLEFQILTRNVNFSNKRTLRLFQKVQIP 757
            LFTS   G    +L  I+   S  +N  L++ F  +E+KTAL  M  TKAPGPDG P LFF+       + VKNF L    + R +N+++   + L  KV+ P
Sbjct:    2 LFTSQGRGSTQEVLNSIEPRVSISMNNTLNADFTDEEVKTALFQMHPTKAPGPDGMPPLFFQ------NRAVKNF-LHLDRILRKINYTH---VALIPKVKNP 94          

HSP 3 Score: 25.0238 bits (53), Expect = 4.844e-99
Identity = 13/31 (41.94%), Postives = 19/31 (61.29%), Query Frame = 2

Query: 2210 ILRIPLAREPHDDFMVCSGEPSGEFSVRSAY 2302
            I  IPL+ +   D ++   +  G+FSVRSAY
Sbjct:  539 IHSIPLSIQHPLDQLIWHYDRKGQFSVRSAY 569          

BLAST of g.barbadense_cottongen_reftransV1_0092164 vs. ExPASy TrEMBL
Match: Q2QXH7_ORYSJ (Os12g0156200 protein OS=Oryza sativa subsp. japonica GN=LOC_Os12g06010 PE=4 SV=2)

HSP 1 Score: 286.189 bits (731), Expect = 5.492e-99
Identity = 162/404 (40.10%), Postives = 226/404 (55.94%), Query Frame = 3

Query:  876 CFHSREII*DNVLLAYEILHSFCQKRTGNRDIWQ*NLILGKLMT-VE*GFLQAIMLQMGFTRDWVALVMKCITTTSYAVNINGSRARIF*PTRGLRQGDPLSPFLFLMCSEGLSLLMRLAMGEGLLKGIKTCRRGLEISHL-FADDCILFDEATTRKAMMIKEILKEYKSCSGQCGNFEKSTVFYSSISKGDMKNEISEFLGVRVSTNLEKYFGLPSMVGR-RKESFLNLKERISWKFEGRSGR-LLSQGVKDIFIKSVLQAIPTYAMSCFFLRNSLCGEIEMIFAKFWWQKGYGKRGIHWCQWKHMCHPKEEYGMGLRNMSQFNIALLAKQG*RLITNPDSLVTRVFKAKYFPNEDFLNSCLGNKNSYVWKNIWATKGILAKGLCWKVGTGLNFSIANDAWIP 2075
             F    +I DN LLA+E  H+  + +  N       L L K    V+  FL+  M ++GF R WV  +MKC+T+  Y V  NG+  + F PTRGLRQGDPL PFLFL  ++GLSLL++  + +  L   K CR    ISHL FADD +LF +A  R+A ++KE+L  Y   +GQ  N  K ++     S   +   ISE L V      ++Y G P+  GR  K  F +L+ +I WK   + G   LS G K++ IK+V+QAIP Y M  F L  S+  ++  +   FWW    G+R  HW  W  +  PK   G+G R+   FN ALLA+Q  RLIT PDSL  RV KAKYFP+   +++  G+ +S  W++I     +L KG+ W+VG G +  I  D+W+P
Sbjct:  670 AFVQGRMITDNALLAFECFHAMQKNKKANHAACAYKLDLSKAYDRVDWRFLEMAMNKLGFARRWVNWIMKCVTSVRYMVKFNGTLLQSFAPTRGLRQGDPLLPFLFLFVADGLSLLLKEKVAQNSLTPFKVCRAAPGISHLLFADDTLLFFKAHQREAEVVKEVLSSYAMGTGQLINPAKCSILMGGASTPAVSEAISEILQVERDRFEDRYLGFPTPEGRMHKGRFQSLQAKI-WKRVIQWGENHLSTGGKEVLIKAVIQAIPVYVMGIFKLPESVIDDLTKLTKNFWWDSMNGQRKTHWKAWDSLTKPKSLGGLGFRDYRLFNQALLARQAWRLITYPDSLCARVLKAKYFPHGSLIDTSFGSNSSPAWRSIEYGLDLLKKGIIWRVGNGNSIRIWRDSWLP 1072          

HSP 2 Score: 59.6918 bits (143), Expect = 5.492e-99
Identity = 29/64 (45.31%), Postives = 39/64 (60.94%), Query Frame = 1

Query:  706 EFFKQTYIALIPKSPNPRTLVNFRPISLCTVIYKFVAKAITNCLQDVIG*CIYQAQSAFILGRL 897
            E    T I LIPK   P  L +FRPISLC V+YK V+K + N L+ ++   +   QSAF+ GR+
Sbjct:  613 EGVNDTAIVLIPKKEQPVDLRDFRPISLCNVVYKVVSKCLVNRLRPILDDLVSVEQSAFVQGRM 676          

HSP 3 Score: 53.1434 bits (126), Expect = 5.492e-99
Identity = 26/86 (30.23%), Postives = 47/86 (54.65%), Query Frame = 2

Query:  407 SLEISEAATIFFQRLFTSNIIGDLSHLLMGIKKSFSYELNTKLSSPFNAKEIKTALQGMESTKAPGPDGFPALFFRRYWHIAGKDV 664
            ++++   AT +FQ ++T++   +   +   I++  +  +N KL   F   EI  A+  +   K+PGPDGFPA F++R W     D+
Sbjct:  513 TMKLESMATEYFQDVYTADPNLNPETVTRLIQEKVTDIMNEKLCEDFTEDEISQAIFQIGPLKSPGPDGFPARFYQRNWGTIKADI 598          

HSP 4 Score: 35.039 bits (79), Expect = 5.492e-99
Identity = 22/55 (40.00%), Postives = 28/55 (50.91%), Query Frame = 1

Query:  253 LKRLLYMEIDKHKMY*EQMARVNWLKFGYKNFVFFHKFTSARKRRNIISMLELRN 417
            +  LLY E    +M   Q +RVNWLK   +N  FFH     R ++N IS L   N
Sbjct:  456 MNELLYRE----EMLWLQRSRVNWLKDEDRNTKFFHSRAVWRAKKNKISKLRDAN 506