g.barbadense_cottongen_reftransV1_0092022, g.barbadense_cottongen_reftransV1_0092022 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0092022 vs. ExPASy Swiss-Prot
Match: POLX_TOBAC (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 9.325e-49
Identity = 99/292 (33.90%), Postives = 160/292 (54.79%), Query Frame = 3

Query: 1998 NTVCRFQKSLYGLKQASRQWNLKLTQALVSARFQQSKYDYSLFTKQ-QGGNXXXXXXXXXXXXXTGNDEVLIKELKGILNQNFKMKDLGELKYFLGLEILRFKDG--IFLNQRKYALELIADSGIGGAKSATTPLEQNTKLTAIEYDQGIGQEEKEDSLLQDKTVYQRLIGRLLY-LTHTRPDITYAVHCLSQFMQHPKVSHLNTALRVVRYIKNSPGQGIXXXXXXXCQLITFCDSDCASCPMSRKSVTGFCIKLGNSLTSWKVKKQNTVARSSAETKYRSMAATASEIVW 2861
            + VC+  KSLYGLKQA RQW +K    + S  + ++  D  ++ K+    N ++LL+YVDD++I G D+ LI +LKG L+++F MKDLG  +  LG++I+R +    ++L+Q KY   ++    +  AK  +TPL  + KL+       +  EEK +     K  Y   +G L+Y +  TRPDI +AV  +S+F+++P   H      ++RY++ + G  +    S    L  + D+D A    +RKS TG+         SW+ K Q  VA S+ E +Y +   T  E++W
Sbjct:  951 HMVCKLNKSLYGLKQAPRQWYMKFDSFMKSQTYLKTYSDPCVYFKRFSENNFIILLLYVDDMLIVGKDKGLIAKLKGDLSKSFDMKDLGPAQQILGMKIVRERTSRKLWLSQEKYIERVLERFNMKNAKPVSTPLAGHLKLSKKMCPTTV--EEKGN---MAKVPYSSAVGSLMYAMVCTRPDIAHAVGVVSRFLENPGKEHWEAVKWILRYLRGTTGDCLCFGGSDPI-LKGYTDADMAGDIDNRKSSTGYLFTFSGGAISWQSKLQKCVALSTTEAEYIAATETGKEMIW 1236          

HSP 2 Score: 77.0258 bits (188), Expect = 9.325e-49
Identity = 47/119 (39.50%), Postives = 75/119 (63.03%), Query Frame = 2

Query: 1589 EPKSYSEAVQNPA*---IKAMEEEISTLESNNTWTVVPLPLXXXXXXXXXXXXXXYKASSEVERFKARLVAKGYSQKEGMGYVDTFSPVAKLVTVRVVLALAFIFRWPLFQMDVFNAFL 1936
            EP+S  E + +P     +KAM+EE+ +L+ N T+ +V LP  K  + CK+  K+K     ++ R+KARLV KG+ QK+G+ + + FSPV K+ ++R +L+LA      + Q+DV  AFL
Sbjct:  810 EPESLKEVLSHPEKNQLMKAMQEEMESLQKNGTYKLVELPKGKRPLKCKWVFKLKKDGDCKLVRYKARLVVKGFEQKKGIDFDEIFSPVVKMTSIRTILSLAASLDLEVEQLDVKTAFL 928          

HSP 3 Score: 26.1794 bits (56), Expect = 9.325e-49
Identity = 10/14 (71.43%), Postives = 11/14 (78.57%), Query Frame = 1

Query: 1942 DLCEEVYMELPTGF 1983
            DL EE+YME P GF
Sbjct:  931 DLEEEIYMEQPEGF 944          

HSP 4 Score: 105.145 bits (261), Expect = 1.355e-41
Identity = 57/140 (40.71%), Postives = 79/140 (56.43%), Query Frame = 2

Query:  788 GIIHQSLCVHTPQQNGVTERKHRHLLEVAKALKFQSKVLLKFWGECVKTACYLIN*LPSSVLHNKFPFEILYNIQPNLSHLKVFGCLCYA-TQTPYKDKFSPKVIPSVFMGYSSTQKGYILFNLETKSFFVNRDVKFHES 1204
            GI H+     TPQ NGV ER +R ++E  +++   +K+   FWGE V+TACYLIN  PS  L  + P  +  N + + SHLKVFGC  +A      + K   K IP +F+GY   + GY L++   K    +RDV F ES
Sbjct:  568 GIRHEKTVPGTPQHNGVAERMNRTIVEKVRSMLRMAKLPKSFWGEAVQTACYLINRSPSVPLAFEIPERVWTNKEVSYSHLKVFGCRAFAHVPKEQRTKLDDKSIPCIFIGYGDEEFGYRLWDPVKKKVIRSRDVVFRES 707          

HSP 5 Score: 88.9669 bits (219), Expect = 1.355e-41
Identity = 44/133 (33.08%), Postives = 67/133 (50.38%), Query Frame = 3

Query:  357 SLWYARLGHVPSSKLHHVPFLQSFSF-DDNLIHSCAVCPLARQTRQPFSNSVTRSAKPFDLVHLDIWGPYRISTHSGHRYFLTIVDDHSRMTWVYLLKHKSEALLYLKQFIHLVKNQFSAFIKYFRSDNGSKF 752
             LW+ R+GH+    L  +      S+     +  C  C   +Q R  F  S  R     DLV+ D+ GP  I +  G++YF+T +DD SR  WVY+LK K +     ++F  LV+ +    +K  RSDNG ++
Sbjct:  423 DLWHKRMGHMSEKGLQILAKKSLISYAKGTTVKPCDYCLFGKQHRVSFQTSSERKLNILDLVYSDVCGPMEIESMGGNKYFVTFIDDASRKLWVYILKTKDQVFQVFQKFHALVERETGRKLKRLRSDNGGEY 555          

BLAST of g.barbadense_cottongen_reftransV1_0092022 vs. ExPASy Swiss-Prot
Match: M810_ARATH (Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana GN=AtMg00810 PE=4 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.245e-44
Identity = 87/227 (38.33%), Postives = 136/227 (59.91%), Query Frame = 3

Query: 2187 TGNDEVLIKELKGILNQNFKMKDLGELKYFLGLEILRFKDGIFLNQRKYALELIADSGIGGAKSATTPL--EQNTKLTAIEYDQGIGQEEKEDSLLQDKTVYQRLIGRLLYLTHTRPDITYAVHCLSQFMQHPKVSHLNTALRVVRYIKNSPGQGIXXXXXXXCQLITFCDSDCASCPMSRKSVTGFCIKLGNSLTSWKVKKQNTVARSSAETKYRSMAATASEIVW 2861
            TG+   L+  L   L+  F MKDLG + YFLG++I     G+FL+Q KYA +++ ++G+   K  +TPL  + N+ ++  +Y               D + ++ ++G L YLT TRPDI+YAV+ + Q M  P ++  +   RV+RY+K +   G+ +  +S   +  FCDSD A C  +R+S TGFC  LG ++ SW  K+Q TV+RSS ET+YR++A TA+E+ W
Sbjct:   13 TGSSNTLLNMLIFQLSSTFSMKDLGPVHYFLGIQIKTHPSGLFLSQTKYAEQILNNAGMLDCKPMSTPLPLKLNSSVSTAKY--------------PDPSDFRSIVGALQYLTLTRPDISYAVNIVCQRMHEPTLADFDLLKRVLRYVKGTIFHGLYIHKNSKLNVQAFCDSDWAGCTSTRRSTTGFCTFLGCNIISWSAKRQPTVSRSSTETEYRALALTAAELTW 225          

BLAST of g.barbadense_cottongen_reftransV1_0092022 vs. ExPASy Swiss-Prot
Match: COPIA_DROME (Copia protein OS=Drosophila melanogaster GN=GIP PE=1 SV=3)

HSP 1 Score: 127.102 bits (318), Expect = 1.706e-42
Identity = 89/296 (30.07%), Postives = 148/296 (50.00%), Query Frame = 3

Query: 1995 GNTVCRFQKSLYGLKQASRQWNLKLTQALVSARFQQSKYDYSLFTKQQGG---NXXXXXXXXXXXXXTGNDEVLIKELKGILNQNFKMKDLGELKYFLGLEILRFKDGIFLNQRKYALELIADSGIGGAKSATTPLEQNTKLTAIEYDQGIGQEEKEDSLLQDKTVYQRLIGRLLYLTH-TRPDITYAVHCLSQFMQHPKVSHLNTALRVVRYIKNSPGQGIXXXXXXXCQ--LITFCDSDCASCPMSRKSVTGFCIKLGN-SLTSWKVKKQNTVARSSAETKYRSMAATASEIVW 2861
             + VC+  K++YGLKQA+R W     QAL    F  S  D  ++   +G    N+ +LL   D ++ TG D   +   K  L + F+M DL E+K+F+G+ I   +D I+L+Q  Y  ++++   +    + +TPL        +  D+               T  + LIG L+Y+   TRPD+T AV+ LS++             RV+RY+K +    ++   + + +  +I + DSD A   + RKS TG+  K+ + +L  W  K+QN+VA SS E +Y ++     E +W
Sbjct: 1028 SDNVCKLNKAIYGLKQAARCWFEVFEQALKECEFVNSSVDRCIYILDKGNINENIYVLLYVDDVVIATG-DMTRMNNFKRYLMEKFRMTDLNEIKHFIGIRIEMQEDKIYLSQSAYVKKILSKFNMENCNAVSTPLPSKINYELLNSDEDCN------------TPCRSLIGCLMYIMLCTRPDLTTAVNILSRYSSKNNSELWQNLKRVLRYLKGTIDMKLIFKKNLAFENKIIGYVDSDWAGSEIDRKSTTGYLFKMFDFNLICWNTKRQNSVAASSTEAEYMALFEAVREALW 1310          

HSP 2 Score: 68.5514 bits (166), Expect = 1.706e-42
Identity = 37/101 (36.63%), Postives = 62/101 (61.39%), Query Frame = 2

Query: 1634 KAMEEEISTLESNNTWTVVPLPLXXXXXXXXXXXXXXYKASSEVERFKARLVAKGYSQKEGMGYVDTFSPVAKLVTVRVVLALAFIFRWPLFQMDVFNAFL 1936
            +A+  E++  + NNTWT+   P  K+ +  ++   +KY       R+KARLVA+G++QK  + Y +TF+PVA++ + R +L+L   +   + QMDV  AFL
Sbjct:  908 EAINTELNAHKINNTWTITKRPENKNIVDSRWVFSVKYNELGNPIRYKARLVARGFTQKYQIDYEETFAPVARISSFRFILSLVIQYNLKVHQMDVKTAFL 1008          

HSP 3 Score: 21.9422 bits (45), Expect = 1.706e-42
Identity = 8/12 (66.67%), Postives = 9/12 (75.00%), Query Frame = 1

Query: 1945 LCEEVYMELPTG 1980
            L EE+YM LP G
Sbjct: 1012 LKEEIYMRLPQG 1023          

HSP 4 Score: 97.4413 bits (241), Expect = 2.193e-34
Identity = 60/167 (35.93%), Postives = 86/167 (51.50%), Query Frame = 2

Query:  788 GIIHQSLCVHTPQQNGVTERKHRHLLEVAKALKFQSKVLLKFWGECVKTACYLIN*LPSSVL--HNKFPFEILYNIQPNLSHLKVFGCLCYATQTPYKDKFSPKVIPSVFMGYSSTQKGYILFNLETKSFFVNRDVKFHESVFPFHFAPIDSSVFFPPQLQILDSDF 1282
            GI +     HTPQ NGV+ER  R + E A+ +   +K+   FWGE V TA YLIN +PS  L   +K P+E+ +N +P L HL+VFG   Y      + KF  K   S+F+GY     G+ L++   + F V RDV   E+      A    +VF     +  + +F
Sbjct:  568 GISYHLTVPHTPQLNGVSERMIRTITEKARTMVSGAKLDKSFWGEAVLTATYLINRIPSRALVDSSKTPYEMWHNKKPYLKHLRVFGATVYVHIKNKQGKFDDKSFKSIFVGYEPN--GFKLWDAVNEKFIVARDVVVDETNMVNSRAVKFETVFLKDSKESENKNF 732          

HSP 5 Score: 72.4034 bits (176), Expect = 2.193e-34
Identity = 46/157 (29.30%), Postives = 71/157 (45.22%), Query Frame = 3

Query:  315 HSIDCMHSVLDNNTSLWYARLGHVPSSKLHHVPFLQSFS---FDDNLIHSCAVCPL---ARQTRQPFSN--SVTRSAKPFDLVHLDIWGPYRISTHSGHRYFLTIVDDHSRMTWVYLLKHKSEALLYLKQFIHLVKNQFSAFIKYFRSDNGSKFFNS 761
            +SI+  H    NN  LW+ R GH+   KL  +     FS     +NL  SC +C      +Q R PF      T   +P  +VH D+ GP    T     YF+  VD  +     YL+K+KS+     + F+   +  F+  + Y   DNG ++ ++
Sbjct:  405 YSINAKHK---NNFRLWHERFGHISDGKLLEIKRKNMFSDQSLLNNLELSCEICEPCLNGKQARLPFKQLKDKTHIKRPLFVVHSDVCGPITPVTLDDKNYFVIFVDQFTHYCVTYLIKYKSDVFSMFQDFVAKSEAHFNLKVVYLYIDNGREYLSN 558          

BLAST of g.barbadense_cottongen_reftransV1_0092022 vs. ExPASy Swiss-Prot
Match: YCH4_YEAST (Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TY5A PE=5 SV=2)

HSP 1 Score: 105.531 bits (262), Expect = 5.184e-23
Identity = 86/296 (29.05%), Postives = 142/296 (47.97%), Query Frame = 3

Query: 1980 FLQSRG-NTVCRFQKSLYGLKQASRQWNLKLTQALVSARFQQSKYDYSLFTKQQGGNXXXXXXXXXXXXXTGNDEVLIKELKGILNQNFKMKDLGELKYFLGLEILRFKDG-IFLNQRKYALELIADSGIGGAKSATTPLEQNTKLTAIEYDQGIGQEEKEDSLLQDKTVYQRLIGRLLYLTHT-RPDITYAVHCLSQFMQHPKVSHLNTALRVVRYIKNSPGQGIXXXXXXXCQLITFCDSDCASCPMSRKSVTGFCIKLGNSLTSWKVKK-QNTVARSSAETKYRSMAATASEI 2855
            F+  R  + V      +YGLKQA   WN  +   L    F + + ++ L+ +      + + +YVDDL++      +   +K  L + + MKDLG++  FLGL I +  +G I L+ + Y  +  ++S I   K   TPL  +  L            E     L+D T YQ ++G+LL+  +T RPDI+Y V  LS+F++ P+  HL +A RV+RY+  +    +   S S   L  +CD+   +      S  G+   L  +  +W  KK +  +   S E +Y + + T  EI
Sbjct:   24 FVNERNPDYVWELYGGMYGLKQAPLLWNEHINNTLKKIGFCRHEGEHGLYFRSTSDGPIYIAVYVDDLLVAAPSPKIYDRVKQELTKLYSMKDLGKVDKFLGLNIHQSSNGDITLSLQDYIAKAASESEINTFKLTQTPLCNSKPLF-----------ETTSPHLKDITPYQSIVGQLLFCANTGRPDISYPVSLLSRFLREPRAIHLESARRVLRYLYTTRSMCLKYRSGSQLALTVYCDASHGAIHDLPHSTGGYVTLLAGAPVTWSSKKLKGVIPVPSTEAEYITASETVMEI 308          

BLAST of g.barbadense_cottongen_reftransV1_0092022 vs. ExPASy Swiss-Prot
Match: M240_ARATH (Uncharacterized mitochondrial protein AtMg00240 OS=Arabidopsis thaliana GN=AtMg00240 PE=4 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 2.885e-16
Identity = 39/79 (49.37%), Postives = 55/79 (69.62%), Query Frame = 3

Query: 2508 LYLTHTRPDITYAVHCLSQFMQHPKVSHLNTALRVVRYIKNSPGQGIXXXXXXXCQLITFCDSDCASCPMSRKSVTGFC 2744
            +YLT TRPD+T+AV+ LSQF    + + +    +V+ Y+K + GQG+  S++S  QL  F DSD ASCP +R+SVTGFC
Sbjct:    1 MYLTITRPDLTFAVNRLSQFSSASRTAQMQAVYKVLHYVKGTVGQGLFYSATSDLQLKAFADSDWASCPDTRRSVTGFC 79          

BLAST of g.barbadense_cottongen_reftransV1_0092022 vs. ExPASy Swiss-Prot
Match: M820_ARATH (Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana GN=AtMg00820 PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 2.323e-15
Identity = 43/105 (40.95%), Postives = 70/105 (66.67%), Query Frame = 2

Query: 1571 STSSLVEPKSYSEAVQNPA*IKAMEEEISTLESNNTWTVVPLPLXXXXXXXXXXXXXXYKASSEVERFKARLVAKGYSQKEGMGYVDTFSPVAKLVTVRVVLALA 1885
            +T+   EPKS   A+++P   +AM+EE+  L  N TW +VP P+ ++ + CK+  K K  +   ++R KARLVAKG+ Q+EG+ +V+T+SPV +  T+R +L +A
Sbjct:   21 TTTIKKEPKSVIFALKDPGWCQAMQEELDALSRNKTWILVPPPVNQNILGCKWVFKTKLHSDGTLDRLKARLVAKGFHQEEGIYFVETYSPVVRTATIRTILNVA 125          

BLAST of g.barbadense_cottongen_reftransV1_0092022 vs. ExPASy TrEMBL
Match: A5BC87_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006992 PE=4 SV=1)

HSP 1 Score: 271.552 bits (693), Expect = 1.553e-103
Identity = 141/286 (49.30%), Postives = 199/286 (69.58%), Query Frame = 3

Query: 2004 VCRFQKSLYGLKQASRQWNLKLTQALVSARFQQSKYDYSLFTKQQGGNXXXXXXXXXXXXXTGNDEVLIKELKGILNQNFKMKDLGELKYFLGLEILRFKDGIFLNQRKYALELIADSGIGGAKSATTPLEQNTKLTAIEYDQGIGQEEKEDSLLQDKTVYQRLIGRLLYLTHTRPDITYAVHCLSQFMQHPKVSHLNTALRVVRYIKNSPGQGIXXXXXXXCQLITFCDSDCASCPMSRKSVTGFCIKLGNSLTSWKVKKQNTVARSSAETKYRSMAATASEIVW 2861
            VCR  KSLYGLKQASR W  K +  +    F QS  DYSLFTK  G + +++LIYVDD++ITGNDE +I  LK  L+  F++KDLG+L+YFLG+E+ R  DGI ++QRKY L+++ ++G+ GAK   TP+E+N KL     D           LL++ + Y+RL+G+L+YLT TR +I+Y+VH LSQFMQ P+  HL+    +++Y+K +PGQG+   +  +  L  FCD+D A C ++R+SVTG+CI LG +L SWK KKQ TV+RSSAE++YR+MA+   E+ W
Sbjct:  760 VCRLNKSLYGLKQASRSWFQKFSATIQQNGFHQSMADYSLFTKISGNSFIVVLIYVDDMIITGNDENVIAALKESLHTKFRIKDLGQLRYFLGIEVARSTDGILISQRKYTLDILDEAGLLGAKPLLTPMEENNKLLPTVGD-----------LLKNPSTYRRLVGQLIYLTITRXEISYSVHILSQFMQEPRKPHLHVVHHLLQYLKGAPGQGLYFPAKGNLLLRGFCDADWARCSITRRSVTGYCIFLGGALISWKTKKQTTVSRSSAESEYRAMASITCELTW 1034          

HSP 2 Score: 121.709 bits (304), Expect = 1.553e-103
Identity = 66/139 (47.48%), Postives = 94/139 (67.63%), Query Frame = 2

Query: 1523 HIFYSHLPTHNQLFLSSTSSLVEPKSYSEAVQNPA*IKAMEEEISTLESNNTWTVVPLPLXXXXXXXXXXXXXXYKASSEVERFKARLVAKGYSQKEGMGYVDTFSPVAKLVTVRVVLALAFIFRWPLFQMDVFNAFLQ 1939
            +I Y+ L    + F+ + ++LVEP +Y +AV +P   +AM  E+  LE N+TWT+ PLP     I CK+  KIKY +   VER+KA+LVAKG++Q+EG+ Y +TFSP+AKL T+R +LA+A +  W   QMDV NAFL 
Sbjct:  598 YISYAQLSPKYRNFVCAITTLVEPTTYEQAVLDPKWQEAMAAELHALEQNHTWTLTPLPSGHRPIGCKWVYKIKYNSDGTVERYKAQLVAKGFTQREGIDYKETFSPMAKLTTIRCLLAIAAVRHWSFHQMDVQNAFLH 736          

HSP 3 Score: 35.4242 bits (80), Expect = 1.553e-103
Identity = 16/23 (69.57%), Postives = 17/23 (73.91%), Query Frame = 1

Query: 1942 DLCEEVYMELPTGFCSQEGTLFV 2010
            DL EEVYM+LP GFC Q  TL V
Sbjct:  738 DLLEEVYMQLPPGFCRQGETLMV 760          

BLAST of g.barbadense_cottongen_reftransV1_0092022 vs. ExPASy TrEMBL
Match: A5BNR5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_035665 PE=4 SV=1)

HSP 1 Score: 287.73 bits (735), Expect = 2.160e-101
Identity = 157/288 (54.51%), Postives = 197/288 (68.40%), Query Frame = 3

Query: 1998 NTVCRFQKSLYGLKQASRQWNLKLTQALVSARFQQSKYDYSLFTKQQGGNXXXXXXXXXXXXXTGNDEVLIKELKGILNQNFKMKDLGELKYFLGLEILRFKDGIFLNQRKYALELIADSGIGGAKSATTPLEQNTKLTAIEYDQGIGQEEKEDSLLQDKTVYQRLIGRLLYLTHTRPDITYAVHCLSQFMQHPKVSHLNTALRVVRYIKNSPGQGIXXXXXXXCQLITFCDSDCASCPMSRKSVTGFCIKLGNSLTSWKVKKQNTVARSSAETKYRSMAATASEIVW 2861
            N VCR +KS+YGLKQASR W    T  + SA + QSK DYSLFTK QG     +LIYVDD+++TGND   IK LK  L + F +KDLGELKYFLG+E  R K GIF++QRKY L+++ D+G+ G K    P+EQN KLT            ++  LL D + Y+RL+GRL+YLT TRPDI Y+V  LSQFM  P+  H   ALRV+RYIK SPGQG+ L S ++  L  FCDSD   C MSR+SV+G+C+ LG+SL SWK KKQ  V+RSSAE +YR+MA T  E+ W
Sbjct: 1356 NLVCRLRKSIYGLKQASRNWFSTFTATVKSAGYIQSKADYSLFTKSQGNKFTAILIYVDDILLTGNDLHEIKMLKTHLLKRFFIKDLGELKYFLGIEFSRSKKGIFMSQRKYTLDILQDTGLTGVKPEKFPMEQNLKLT-----------NEDGELLHDPSRYRRLVGRLIYLTVTRPDIVYSVRTLSQFMNTPRKPHWEAALRVLRYIKGSPGQGLFLPSENNLTLSAFCDSDWGGCRMSRRSVSGYCVFLGSSLISWKSKKQTNVSRSSAEAEYRAMANTCLELTW 1632          

HSP 2 Score: 112.079 bits (279), Expect = 2.160e-101
Identity = 72/181 (39.78%), Postives = 104/181 (57.46%), Query Frame = 2

Query: 1424 PLCCFSRIFKPPNWLHDXXXXXXXXXXXXXXXN----------HIFYSHLPTHNQLFLSSTSSLVEPKSYSEAVQNPA*IKAMEEEISTLESNNTWTVVPLPLXXXXXXXXXXXXXXYKASSEVERFKARLVAKGYSQKEGMGYVDTFSPVAKLVTVRVVLALAFIFRWPLFQMDVFNAFL 1936
            PL   +R  +PP W HD++ S   + S    S+          H+ +     H++ FL+  ++  EP S+ +A  +P   +AM  E+  LE NNTW +VPLP     I C++  KIKY +   +ER+KARLVAKGY+Q  G+ Y +TFSP AKL T+R +L +A    W + Q+DV NAFL
Sbjct: 1154 PLRRSTRHIQPPAWHHDYVMSAQLNHSSTQSSSRQGTRYPLSSHLSFFRFSPHHRAFLALLTAQTEPSSFEQADCDPRWRQAMSTELQALERNNTWEMVPLPPGHKPIGCRWVYKIKYHSDGTIERYKARLVAKGYTQVAGIDYQETFSPTAKLTTLRCLLTVAASRNWYIHQLDVHNAFL 1334          

HSP 3 Score: 164.851 bits (416), Expect = 7.723e-68
Identity = 78/140 (55.71%), Postives = 102/140 (72.86%), Query Frame = 2

Query:  788 GIIHQSLCVHTPQQNGVTERKHRHLLEVAKALKFQSKVLLKFWGECVKTACYLIN*LPSSVLHNKFPFEILYNIQPNLSHLKVFGCLCYATQTPYKDKFSPKVIPSVFMGYSSTQKGYILFNLETKSFFVNRDVKFHESV 1207
            GI  Q+ C++TPQQNGV ERKHRH+L VA++L FQS V L+FWGECV TA YLIN +P+ +L NK PFE+LYN  P+L+HL+VFGC CY T    K KF P+    VF+GY   +KGY + +L+T+   V+RDV F E++
Sbjct:  909 GIELQTSCIYTPQQNGVVERKHRHILNVARSLMFQSNVPLEFWGECVLTAVYLINRIPTPLLSNKSPFEVLYNRPPSLTHLRVFGCECYVTNVHPKQKFDPRASICVFLGYPHGKKGYKVLDLQTQKISVSRDVFFRENI 1048          

HSP 4 Score: 122.865 bits (307), Expect = 7.723e-68
Identity = 70/188 (37.23%), Postives = 96/188 (51.06%), Query Frame = 3

Query:  192 LFDGKMKGIGKESRGLYXXXXXXXXXXXXXXXXXXXXXXNIHSIDCMHSVLDNNTSLWYARLGHVPSSKLHHVPFLQSFSFDDNLIHSCAVCPLARQTRQPFSNSVTRSAKPFDLVHLDIWGPYRISTHSGHRYFLTIVDDHSRMTWVYLLKHKSEALLYLKQFIHLVKNQFSAFIKYFRSDNGSKFF 755
            L  GKM G GK+  GLY    S+  S +                      +   + LW+ RLGH   S+   +  L        + + C +CP A+QTR PF  S   +  PF L+H D+WGP++I  H+G RYFLTIVDD SR TW++L+ HKSE    L  F+  VK QF   ++  R DNG++F 
Sbjct:  733 LVSGKMIGSGKQRGGLYYMHPSTNKSVVFH--------------------VSQPSDLWHLRLGHPSFSRFKLLSRLLP-DIHKEIGNHCPICPQAKQTRLPFPKSSITTKFPFSLLHCDVWGPHKIPAHTGSRYFLTIVDDFSRCTWIFLMHHKSETQSLLTNFVQFVKTQFHTDVQTVRMDNGTEFI 899          

BLAST of g.barbadense_cottongen_reftransV1_0092022 vs. ExPASy TrEMBL
Match: A5ARV9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010143 PE=4 SV=1)

HSP 1 Score: 283.493 bits (724), Expect = 7.095e-101
Identity = 160/311 (51.45%), Postives = 207/311 (66.56%), Query Frame = 3

Query: 1944 FV*GGLYGVAYW-----FLQSRGNTVCRFQKSLYGLKQASRQWNLKLTQALVSARFQQSKYDYSLFTKQQGGNXXXXXXXXXXXXXTGNDEVLIKELKGILNQNFKMKDLGELKYFLGLEILRFKDGIFLNQRKYALELIADSGIGGAKSATTPLEQNTKLTAIEYDQGIGQEEKEDSLLQDKTVYQRLIGRLLYLTHTRPDITYAVHCLSQFMQHPKVSHLNTALRVVRYIKNSPGQGIXXXXXXXCQLITFCDSDCASCPMSRKSVTGFCIKLGNSLTSWKVKKQNTVARSSAETKYRSMAATASEIVW 2861
            F+ G LY   Y      F +   + VC+  KSLYGLKQASRQW LKL+ AL +A F+QS  DYSLF +   G    LL+YVDD+++ GN    I E K  L  +FK+KD+G+L+YFLG+E+ R K GI L QRKYALEL+ D+G  GAK +  P+EQ+  LT     +G G E      L+D + Y+RL+GRL+YLT TRPD+ YAVH LSQFM  P+  HL+ A +V+RY+K +PGQGI L S+   +L  +CD+D A C  +R+S TG+CI  GN+  SWK KKQ TV+RSSAE +YRSMA T  EI W
Sbjct: 1082 FLNGDLYEDVYMQLPPGFGRKGEHRVCKLHKSLYGLKQASRQWFLKLSSALKAAGFKQSWSDYSLFXRNTQGRFTTLLVYVDDVILAGNSLEDIIETKQFLASHFKLKDMGQLRYFLGIEVARSKQGIVLCQRKYALELLEDAGFLGAKPSRFPVEQSLTLT-----RGDGAE------LKDASQYRRLVGRLIYLTITRPDLVYAVHILSQFMDTPRQPHLDAAYKVLRYVKQTPGQGIFLPSTGQLELTAYCDADWARCKDTRRSTTGYCIFFGNAPISWKTKKQGTVSRSSAEAEYRSMATTCCEITW 1381          

HSP 2 Score: 114.775 bits (286), Expect = 7.095e-101
Identity = 68/137 (49.64%), Postives = 91/137 (66.42%), Query Frame = 2

Query: 1526 IFYSHLPTHNQLFLSSTSSLVEPKSYSEAVQNPA*IKAMEEEISTLESNNTWTVVPLPLXXXXXXXXXXXXXXYKASSEVERFKARLVAKGYSQKEGMGYVDTFSPVAKLVTVRVVLALAFIFRWPLFQMDVFNAFL 1936
            + Y  L   ++ F    +   EP+S+S+AV +    +AM  EI  L++N TW++VPLP  K  I CK+  KIKY     +ER+KARLVAKG+SQ EG+ Y +TF+PVAKL TVRV+L+LA I  W L Q+DV NAFL
Sbjct:  947 LSYDRLSPMHKAFTVKITLAKEPRSFSQAVLDSRWREAMNTEIQALQANKTWSLVPLPSHKKPIGCKWVYKIKYNPDGTIERYKARLVAKGFSQVEGIDYRETFAPVAKLTTVRVLLSLASIQGWHLHQLDVNNAFL 1083          

HSP 3 Score: 167.162 bits (422), Expect = 3.830e-78
Identity = 86/184 (46.74%), Postives = 117/184 (63.59%), Query Frame = 2

Query:  755 QFNCSSLFTDLGIIHQSLCVHTPQQNGVTERKHRHLLEVAKALKFQSKVLLKFWGECVKTACYLIN*LPSSVLHNKFPFEILYNIQPNLSHLKVFGCLCYATQTPYK-DKFSPKVIPSVFMGYSSTQKGYILFNLETKSFFVNRDVKFHESVFPFHFAPIDSS----VFFPPQLQILDSDFLHI 1291
            +F  +  ++  GI+HQ+ C++TPQQNGV ERKHRHLL VA+AL FQS +   FWG+ + TA YLIN  P+ +L  K PFE L++  PN SHL+VFGC C+ +  P +  KF P+ I SVF+GY   QKGY +++L+ K   ++RDV F E+ FP+      +S     FFP   Q  D D  HI
Sbjct:  658 EFKHTQFYSSRGILHQTSCINTPQQNGVVERKHRHLLNVARALLFQSHLPKPFWGDAILTAAYLINRTPTPLLQGKTPFEKLFHKSPNYSHLRVFGCRCFVSTHPLRPSKFDPRSIESVFIGYPHGQKGYKVYSLKDKKXLISRDVTFFETEFPYQNXLSTTSPSLDTFFPSLPQTPDIDDDHI 841          

HSP 4 Score: 155.221 bits (391), Expect = 3.830e-78
Identity = 86/188 (45.74%), Postives = 111/188 (59.04%), Query Frame = 3

Query:  192 LFDGKMKGIGKESRGLYXXXXXXXXXXXXXXXXXXXXXXNIHSIDCMHSVLDNNTSLWYARLGHVPSSKLHHV-PFLQSFSFDDNLIHSCAVCPLARQTRQPFSNSVTRSAKPFDLVHLDIWGPYRISTHSGHRYFLTIVDDHSRMTWVYLLKHKSEALLYLKQFIHLVKNQFSAFIKYFRSDNGSKF 752
            L  GKM G+G ES GLY                      N+      + V      LW+ RLGH PSSK+  + PFLQ+ + D   + +C++CPLA+ TR PF  SV+ S   FDL+H+DIWG Y + + SG +YFLTIVDDHSR TWVYL+ HKSEA   L  F++LV NQF + +K  RSDNG +F
Sbjct:  496 LQSGKMIGMGTESEGLYCL--------------------NLPRKGTCNVVNTKTQDLWHQRLGH-PSSKVSVLFPFLQNKTLD---VSTCSICPLAKHTRTPFPLSVSSSDSCFDLIHVDIWGGYHVPSLSGAQYFLTIVDDHSRSTWVYLMHHKSEARSLLVHFVNLVANQFGSQVKIVRSDNGPEF 659          

BLAST of g.barbadense_cottongen_reftransV1_0092022 vs. ExPASy TrEMBL
Match: Q9LVQ2_ARATH (Retroelement pol polyprotein-like OS=Arabidopsis thaliana PE=4 SV=1)

HSP 1 Score: 286.574 bits (732), Expect = 8.661e-101
Identity = 156/294 (53.06%), Postives = 204/294 (69.39%), Query Frame = 3

Query: 1980 FLQSRGNTVCRFQKSLYGLKQASRQWNLKLTQALVSARFQQSKYDYSLFTKQQGGNXXXXXXXXXXXXXTGNDEVLIKELKGILNQNFKMKDLGELKYFLGLEILRFKDGIFLNQRKYALELIADSGIGGAKSATTPLEQNTKLTAIEYDQGIGQEEKEDSLLQDKTVYQRLIGRLLYLTHTRPDITYAVHCLSQFMQHPKVSHLNTALRVVRYIKNSPGQGIXXXXXXXCQLITFCDSDCASCPMSRKSVTGFCIKLGNSLTSWKVKKQNTVARSSAETKYRSMAATASEIVW 2861
            F  S  + VCR +KSLYGLKQA R W  KL+ AL    F Q   DYS F+    G  + +L+YVDDL+I GNDE ++++ K  L + F MKDLG+LKYFLG+E+ R  DGIFL+QRKYAL++I+DSG  GA+ A TPLEQN  L +   D G         LLQD   ++RL+GRLLYL HTRP+++Y+VH LSQFMQ P+ +HL  A+R+VRY+K SPGQGILLSS+    L  +CDSD  SCP++R+S++ + + LG S  SWK KKQ+TV+ SSAE +YR+M+    EI W
Sbjct: 1112 FRHSHPDKVCRLRKSLYGLKQAPRCWFKKLSDALKRFGFIQGYEDYSFFSYSCKGIELRVLVYVDDLIICGNDEYMVQKFKEYLGRCFSMKDLGKLKYFLGIEVSRGPDGIFLSQRKYALDIISDSGTLGARPAYTPLEQNHHLAS---DDG--------PLLQDPKPFRRLVGRLLYLLHTRPELSYSVHVLSQFMQAPREAHLEAAMRIVRYLKGSPGQGILLSSNKDLTLEVYCDSDFQSCPLTRRSLSAYVVLLGGSPISWKTKKQDTVSHSSAEAEYRAMSVALKEIKW 1394          

HSP 2 Score: 106.301 bits (264), Expect = 8.661e-101
Identity = 58/139 (41.73%), Postives = 86/139 (61.87%), Query Frame = 2

Query: 1520 NHIFYSHLPTHNQLFLSSTSSLVEPKSYSEAVQNPA*IKAMEEEISTLESNNTWTVVPLPLXXXXXXXXXXXXXXYKASSEVERFKARLVAKGYSQKEGMGYVDTFSPVAKLVTVRVVLALAFIFRWPLFQMDVFNAFL 1936
            ++IF       +++FL++ ++  EPK + EAV+      AM +E+  LE N TW +V LP  K +I  ++  K K+ A   VER+KARLV +G +Q EG  Y +TF+PV K+ TVR +L L    +W ++QMDV NAFL
Sbjct:  958 DYIFDECFSAGHKVFLAAITANDEPKHFKEAVKVKVWNDAMYKEVDALEVNKTWDIVDLPTGKVAIGSQWVYKTKFNADGTVERYKARLVVQGNNQIEGEDYTETFAPVVKMTTVRTLLRLVAANQWEVYQMDVHNAFL 1096          

HSP 3 Score: 26.5646 bits (57), Expect = 8.661e-101
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 1

Query: 1942 DLCEEVYMELPTGF 1983
            DL EEVYM+LP GF
Sbjct: 1099 DLEEEVYMKLPPGF 1112          

HSP 4 Score: 160.999 bits (406), Expect = 4.499e-64
Identity = 81/169 (47.93%), Postives = 113/169 (66.86%), Query Frame = 2

Query:  755 QFNC-SSLFTDLGIIHQSLCVHTPQQNGVTERKHRHLLEVAKALKFQSKVLLKFWGECVKTACYLIN*LPSSVLHNKFPFEILYNIQPNLSHLKVFGCLCYATQ-TPYKDKFSPKVIPSVFMGYSSTQKGYILFNLETKSFFVNRDVKFHESVFPFHFAPIDSSVFFPP 1255
            +F C SS F + GI+HQ+ CV TPQQNG  ERKHRH+L V++AL FQ+ + +KFWGE V TA YLIN  PSS+ +   P+E+L+  +P+   L+VFG  CYA + T  KDKF  +    +F+GY   QKG+ +++L T  F V+RDV F E+VFP+     D +++ PP
Sbjct:  640 EFMCLSSYFKEQGIVHQTSCVGTPQQNGRVERKHRHILNVSRALLFQASLPIKFWGEAVMTAAYLINRTPSSIHNGLSPYELLHGCKPDYDQLRVFGSACYAHRVTRDKDKFGERSRLCIFVGYPFGQKGWKVYDLSTNEFIVSRDVVFRENVFPYATNEGD-TIYTPP 807          

HSP 5 Score: 114.005 bits (284), Expect = 4.499e-64
Identity = 67/187 (35.83%), Postives = 92/187 (49.20%), Query Frame = 3

Query:  195 FDGKMKGIGKESRGLYXXXXXXXXXXXXXXXXXXXXXXNIHSIDCMHSVLDNNTSLWYARLGHVPSSKLHHVPFLQSFSFDDNLIHSCAVCPLARQTRQPFSNSVTRSAKPFDLVHLDIWGPYRISTHSGHRYFLTIVDDHSRMTWVYLLKHKSEALLYLKQFIHLVKNQFSAFIKYFRSDNGSKFF 755
            F   + G G+E  G+Y    ++                 +H +D     +  + +LW+ RLGH   S L  +P     S       SC VC  A+QTR+ F +S  +S   F L+H D+WGPYR+ +  G  YFLTIVDD SR  W YLL  KSE    L  F+   + QF   +K  RSDNG++F 
Sbjct:  476 FSRTLIGTGEERDGVYYLTDAATT--------------TVHKVD-----VTTDHALWHQRLGHPSFSVLSSLPLFSGSSCS-VSSRSCDVCFRAKQTREVFPDSSNKSTDCFSLIHCDVWGPYRVPSSCGAVYFLTIVDDFSRSVWTYLLLAKSEVRSVLTNFLAYTEKQFGKSVKIIRSDNGTEFM 642          

BLAST of g.barbadense_cottongen_reftransV1_0092022 vs. ExPASy TrEMBL
Match: A5ALL8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_001215 PE=4 SV=1)

HSP 1 Score: 295.049 bits (754), Expect = 4.960e-100
Identity = 154/292 (52.74%), Postives = 209/292 (71.58%), Query Frame = 3

Query: 1986 QSRGNTVCRFQKSLYGLKQASRQWNLKLTQALVSARFQQSKYDYSLFTKQQGGNXXXXXXXXXXXXXTGNDEVLIKELKGILNQNFKMKDLGELKYFLGLEILRFKDGIFLNQRKYALELIADSGIGGAKSATTPLEQNTKLTAIEYDQGIGQEEKEDSLLQDKTVYQRLIGRLLYLTHTRPDITYAVHCLSQFMQHPKVSHLNTALRVVRYIKNSPGQGIXXXXXXXCQLITFCDSDCASCPMSRKSVTGFCIKLGNSLTSWKVKKQNTVARSSAETKYRSMAATASEIVW 2861
            Q   N VCR  KSLYGLKQASRQW  K ++A+ +A + QS+ DYSLFT+ QG +   LLIYVDD++ITGND + I  +K  L+  F++KDLG+LKYFLG+E+   K+GIF++QRKYALE+I D+G+ GA    TP+E+  +L+           +K D LL+D   Y+RL+GRL+YLT +RPDITY+VH LS+FM  P+  H+  ALRVVRY+KN+PGQG+  SS+S  +L  +CDSD A CP++R+S TG+C+ LG SL SW+ K+Q TV+ SSAE +YR+M     E+ W
Sbjct: 1143 QGEENLVCRLHKSLYGLKQASRQWFSKFSKAIQAAGYVQSRADYSLFTRIQGKSFTALLIYVDDILITGNDSMSIAAIKKFLHSQFRLKDLGDLKYFLGIEVSSSKNGIFISQRKYALEIIKDAGLLGAAPIDTPMERGLRLS-----------DKSD-LLKDPGHYRRLVGRLIYLTVSRPDITYSVHVLSRFMHQPRKLHMEAALRVVRYLKNAPGQGLFFSSNSDFRLRAYCDSDWAGCPITRRSTTGYCVFLGPSLVSWRSKRQKTVSLSSAEAEYRAMTGACCELTW 1422          

HSP 2 Score: 100.138 bits (248), Expect = 4.960e-100
Identity = 53/101 (52.48%), Postives = 74/101 (73.27%), Query Frame = 2

Query: 1634 KAMEEEISTLESNNTWTVVPLPLXXXXXXXXXXXXXXYKASSEVERFKARLVAKGYSQKEGMGYVDTFSPVAKLVTVRVVLALAFIFRWPLFQMDVFNAFL 1936
            +AM  E+  L++N TWT+  LP  K+ I C++  KIK+++   +ER+KARLVAKG++Q EG+ Y DTFSP+AK++TV  +LALA   RW L Q+DV NAFL
Sbjct: 1024 EAMRSELQALQANGTWTLTSLPTGKTPIGCRWVYKIKHRSDGSIERYKARLVAKGFTQLEGVDYQDTFSPIAKIITVCCLLALAAARRWSLHQLDVNNAFL 1124          

HSP 3 Score: 172.17 bits (435), Expect = 6.595e-74
Identity = 83/149 (55.70%), Postives = 104/149 (69.80%), Query Frame = 2

Query:  770 SLFTDLGIIHQSLCVHTPQQNGVTERKHRHLLEVAKALKFQSKVLLKFWGECVKTACYLIN*LPSSVLHNKFPFEILYNIQPNLSHLKVFGCLCYATQTPYKDKFSPKVIPSVFMGYSSTQKGYILFNLETKSFFVNRDVKFHESVFPF 1216
            S F D G++ Q  CV+TPQQNGV ERKHRH+L+VA+ALKFQ+++  +FWGEC  TA ++IN LPS VL  K PFE LY   P  SHL+VFGCL YAT      KF  + I  +F+GY   QK Y LFNL T+  F +RDV+FHE+ FP+
Sbjct:  681 SFFKDNGVLFQHSCVYTPQQNGVVERKHRHILQVARALKFQAQLPTQFWGECALTAVHIINRLPSPVLSFKTPFERLYLKPPTYSHLRVFGCLAYATNVHVSHKFDHRAIACIFIGYPVGQKAYKLFNLSTRKIFTSRDVRFHENRFPY 829          

HSP 4 Score: 135.961 bits (341), Expect = 6.595e-74
Identity = 78/188 (41.49%), Postives = 112/188 (59.57%), Query Frame = 3

Query:  204 KMKGIGKESRGLYXXXXXXXXXXXXXXXXXXXXXXNIHSIDCMHSVLDNNTSLWYARLGHVPSSKLHHVP--FLQ-SFSFDDNLIHSCAVCPLARQTRQPFSNSVTRSAKPFDLVHLDIWGPYRISTHSGHRYFLTIVDDHSRMTWVYLLKHKSEALLYLKQFIHLVKNQFSAFIKYFRSDNGSKFFN 758
            +M G+GK+  GLY   + +   + +   SS +     +       +  ++T LW++RLGHV  S+L  +   FL  S  F+++    C +C LA+Q R PF  S   S KPFDL+H DIWG Y+ S+ SG  YFLTIVDD++R TW++L+KHK EA   +K F   V  QF++ IK FRSDNG +F +
Sbjct:  502 RMIGLGKQRDGLYYLAALTTNKTETNSYSSPNRPPTCN-------LTTSSTDLWHSRLGHVSHSRLSFIAKNFLNFSIQFNND----CPICLLAKQHRLPFGTSEISSEKPFDLIHCDIWGRYKHSSLSGAHYFLTIVDDYTRFTWIFLMKHKDEAQPLIKHFFSYVSTQFASHIKIFRSDNGGEFLS 678