g.barbadense_cottongen_reftransV1_0094223, g.barbadense_cottongen_reftransV1_0094223 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT11_ARATH (Nucleobase-ascorbate transporter 11 OS=Arabidopsis thaliana GN=NAT11 PE=2 SV=1)

HSP 1 Score: 398.667 bits (1023), Expect = 3.106e-120
Identity = 210/329 (63.83%), Postives = 241/329 (73.25%), Query Frame = 1

Query: 2491 HVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            H + R   VPLS  + W YAFFLT GGAYDY+GC+ +IPSSNIL+DEC+KH YTMKHCR DASNAWRTA+WVRIPYP QWG P FH RTS+IMI VSLVASVDS                                                   VGTYHSAS++V++K PT G+VSRGIALEGFCSLLAGIWGSGTGSTTLTEN HTINITK+ASRRALV GA+FLI+ S +GK+GAILASIP +LAA++LCF+WAL V++GLS L+YTQTASFRNITIVGVSLFLGLSIP YFQQYQP S L+LPSY++P+ AAS GP  T  EQ+ 
Sbjct:  364 HRLFRIYAVPLSALLIWTYAFFLTVGGAYDYRGCNADIPSSNILIDECKKHVYTMKHCRTDASNAWRTASWVRIPYPFQWGFPNFHMRTSIIMIFVSLVASVDS---------------------------------------------------VGTYHSASMIVNAKRPTRGIVSRGIALEGFCSLLAGIWGSGTGSTTLTENIHTINITKVASRRALVIGAMFLIVLSFLGKLGAILASIPQALAASVLCFIWALTVSLGLSNLRYTQTASFRNITIVGVSLFLGLSIPAYFQQYQPLSSLILPSYYIPFGAASSGPFQTGIEQLD 641          

HSP 2 Score: 293.123 bits (749), Expect = 4.372e-83
Identity = 163/209 (77.99%), Postives = 190/209 (90.91%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAVRTLTYHLVYTF 1697
            +K GLRDNPG   LIYYGLQH+LSL GSL+FIP V+VPAM G+DKDTASVISTMLL++G++TILH YFG+RLPLVQGSSF+YLAP LV+IN+ ++RNLTEHKFR  MRELQGAIIVGS+FQ ILGFSGLMSLLLR INPVVVAPTVAAVGLAFFSYGFPQAG+C+E+SVPL+LL+LI TLYLRG+S+FGHRLFRIYAV  L+  L++T+
Sbjct:  175 MKFGLRDNPGFVPLIYYGLQHYLSLVGSLVFIPLVIVPAMDGSDKDTASVISTMLLLTGVTTILHCYFGTRLPLVQGSSFVYLAPVLVVINSEEFRNLTEHKFRDTMRELQGAIIVGSLFQCILGFSGLMSLLLRFINPVVVAPTVAAVGLAFFSYGFPQAGTCVEISVPLILLLLIFTLYLRGVSLFGHRLFRIYAV-PLSALLIWTY 382          

HSP 3 Score: 98.9821 bits (245), Expect = 1.313e-19
Identity = 50/88 (56.82%), Postives = 63/88 (71.59%), Query Frame = 3

Query:  513 KLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNM 776
            K G+   ++EPF+PKKD N  PR+LRSWAK+TGFVS  SGET T++ T +F  S+ F + K GRDQ   GSS K EIDP+LGR RP +
Sbjct:   29 KFGAFFKRVEPFLPKKDLN--PRDLRSWAKKTGFVSDYSGETSTSTRT-KFGESSDFDLPK-GRDQVVTGSSHKTEIDPILGRNRPEI 112          

HSP 4 Score: 81.2629 bits (199), Expect = 3.813e-14
Identity = 41/62 (66.13%), Postives = 55/62 (88.71%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGKF 3846
            QLDF++NA+LS++MVVT L+AF+LDNTVPGS++ERGVY+W+ AED+  DP ++ DYSLP KF
Sbjct:  639 QLDFAMNAVLSLNMVVTFLLAFILDNTVPGSKEERGVYVWTRAEDMQMDPEMRADYSLPRKF 700          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT12_ARATH (Nucleobase-ascorbate transporter 12 OS=Arabidopsis thaliana GN=NAT12 PE=1 SV=3)

HSP 1 Score: 266.929 bits (681), Expect = 5.070e-74
Identity = 159/322 (49.38%), Postives = 202/322 (62.73%), Query Frame = 1

Query: 2491 HVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQY--QPESILMLPSYFVPYAAASDGP 3450
            H I     VPLS+ ITW  AF LT  GAY YKGC P +P SN++   CRK+   MK+CR D S+A  +A W R PYPLQWGVP+F+++ + +M +VS++ASVDS                                                   VG+YH++SLLV+S+PPT GVVSR I LEGF S+LAG+WG+GTGSTTLTEN HTI +TKM SRR +  GA  L++FS+VGKVG  LASIP  + A++LCFMWA+  A+GLS L+Y++  S RNI IVG+SLF  LS+P YFQQY   P S L +PSY+ PY  +S GP
Sbjct:  359 HRIFLIYAVPLSLAITWAAAFLLTETGAYTYKGCDPNVPVSNVVSTHCRKYMTRMKYCRVDTSHALSSAPWFRFPYPLQWGVPLFNWKMAFVMCVVSVIASVDS---------------------------------------------------VGSYHASSLLVASRPPTRGVVSRAIGLEGFTSVLAGLWGTGTGSTTLTENVHTIAVTKMGSRRVVELGACVLVIFSLVGKVGGFLASIPQVMVASLLCFMWAMFTALGLSNLRYSEAGSSRNIIIVGLSLFFSLSVPAYFQQYGISPNSNLSVPSYYQPYIVSSHGP 629          

HSP 2 Score: 225.713 bits (574), Expect = 4.567e-60
Identity = 127/199 (63.82%), Postives = 166/199 (83.42%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLT-EHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV 1664
            +K GLRD PGL  + +YGLQH+LS+ GSLI +P V+VPAMGG+ ++ A+V+ST+L VSGI+T+LH+ FGSRLPL+QG SF++LAPAL IIN+ +++ L   + F+HIMRELQGAII+GS FQ++LG+SGLMSL+LRL+NPVVVAPTVAAVGL+F+SYGFP  G C+E+ V  +LLV+I  LYLR IS+  HR+F IYAV
Sbjct:  169 MKYGLRDTPGLVPIGFYGLQHYLSMLGSLILVPLVIVPAMGGSHEEVANVVSTVLFVSGITTLLHTSFGSRLPLIQGPSFVFLAPALAIINSPEFQGLNGNNNFKHIMRELQGAIIIGSAFQAVLGYSGLMSLILRLVNPVVVAPTVAAVGLSFYSYGFPLVGKCLEIGVVQILLVIIFALYLRKISVLSHRIFLIYAV 367          

HSP 3 Score: 58.9214 bits (141), Expect = 3.077e-7
Identity = 33/64 (51.56%), Postives = 47/64 (73.44%), Query Frame = 1

Query: 3664 LDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLP---GKF 3846
            +++ +N LLSMSMV+  ++A +LDNTVPGS+ ERGVY+WS +E    +P+L  DY LP   G+F
Sbjct:  637 MNYVMNTLLSMSMVIAFIMAVILDNTVPGSKQERGVYVWSDSETATREPALAKDYELPFRVGRF 700          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT1_ARATH (Nucleobase-ascorbate transporter 1 OS=Arabidopsis thaliana GN=NAT1 PE=2 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 4.687e-37
Identity = 109/296 (36.82%), Postives = 144/296 (48.65%), Query Frame = 1

Query: 2497 IVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQY 3384
            I     + + VTI WIYA  LTA GAY  +G                K + T   CR D +N   TA W + PYPLQWG P F    S  M+   LV+ V                                                   +  G Y +AS L  + PP   V+SRGI  +G   LL G++G+GTGST L EN   + +T++ SRR +   A F+I+FS +GK GA+ ASIP+ + AA+ C ++ LV AVGLS LQ+T   S RN+ I G+SLFLG+SIP +F QY
Sbjct:  209 IFERFPILICVTIVWIYAVILTASGAY--RG----------------KPSLTQHSCRTDKANLISTAPWFKFPYPLQWGPPTFSVGHSFAMMSAVLVSMV---------------------------------------------------ESTGAYIAASRLAIATPPPAYVLSRGIGWQGIGVLLDGLFGTGTGSTVLVENVGLLGLTRVGSRRVVQVSAGFMIVFSTLGKFGAVFASIPVPIYAALHCILFGLVAAVGLSFLQFTNMNSMRNLMITGLSLFLGISIPQFFAQY 435          

HSP 2 Score: 128.257 bits (321), Expect = 2.347e-29
Identity = 73/172 (42.44%), Postives = 105/172 (61.05%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLT--EHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            L+  +  NP     +    Q+++ + G+  FIP+++VPAMGG+D D A VI T+L V+GI T+L + FG+RLP V G S  Y+ P   IIN    + ++    +F H MR +QGA+IV S  Q ILG+S +  L  R  +P+ +AP V  VGL  F  GFPQ G+CIE+ +P
Sbjct:   15 LEYCIDSNPPWPETVLLAFQNYILMLGTSAFIPALLVPAMGGSDGDRARVIQTLLFVAGIKTLLQALFGTRLPAVVGGSLAYVVPIAYIINDSSLQKISNDHERFIHTMRAIQGALIVASSIQIILGYSQVWGLFSRFFSPLGMAPVVGLVGLGMFQRGFPQLGNCIEIGLP 186          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT5_ARATH (Nucleobase-ascorbate transporter 5 OS=Arabidopsis thaliana GN=NAT5 PE=2 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 6.971e-37
Identity = 109/325 (33.54%), Postives = 151/325 (46.46%), Query Frame = 1

Query: 2485 GRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHT 3459
            G+HV  R   V  SV I W+YAFFLT GGAY+  G                    T + CR D +     A W+R+P+P QWG P+F    +  M++ S VA V+S   +                                               V  Y SA++      P P V+SRG+  +G   L++G++G+G GS+   EN   + +TK+ SRR +   A F+I FS++GK GA+ ASIP  + AA+ C  +A V A GLS LQ+    SFR + I+G S+FLGLSIP YF ++               A    GPVHT
Sbjct:  212 GKHVFAR-FAVIFSVAIVWLYAFFLTLGGAYNGVGTD------------------TQRSCRTDRAGLISAAPWIRVPWPFQWGAPLFDAGEAFAMMMASFVALVESTGAFIA---------------------------------------------VSRYASATM------PPPSVISRGVGWQGVAILISGLFGTGIGSSVSVENAGLLALTKIGSRRVVQISAGFMIFFSILGKFGAVFASIPSPIIAALYCLFFAYVGAGGLSLLQFCNLNSFRTLFILGFSIFLGLSIPQYFNEHT--------------AIKGYGPVHT 452          

HSP 2 Score: 111.694 bits (278), Expect = 5.654e-24
Identity = 60/151 (39.74%), Postives = 97/151 (64.24%), Query Frame = 3

Query: 1122 GLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTE--HKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIE 1568
            G QH+L + G+ + IPS +VP MGG +++ A +I T+L V+G++T+L + FG+RLP V G+S+ ++   + I+ +  + ++ +   +F+ I+R  QGA+IV S  Q ILGFSGL   ++R ++P+  AP V  VG   +  GFP    CIE
Sbjct:   39 GFQHYLVMLGTTVLIPSALVPQMGGRNEEKAKLIQTILFVAGLNTLLQTVFGTRLPAVIGASYTFVPVTISIMLSGRFNDVADPVERFKRIIRATQGALIVASTLQIILGFSGLWRNVVRFLSPLSAAPLVGLVGYGLYELGFPGVAKCIE 189          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT2_ARATH (Nucleobase-ascorbate transporter 2 OS=Arabidopsis thaliana GN=NAT2 PE=2 SV=2)

HSP 1 Score: 149.443 bits (376), Expect = 5.460e-36
Identity = 106/321 (33.02%), Postives = 153/321 (47.66%), Query Frame = 1

Query: 2497 IVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHT 3459
            +V    + +++ I W YA  LTA GAY +                 R H  T  +CR D SN   +A W++IPYPLQWG P F    +  M+   LV                                                   ++ +  G + +A+ L S+ PP P V+SRGI  +G   LL G++G+ +GS+   EN   +  T++ SRR +   A F+I FS++GK GA+ ASIP ++ AA+ C ++ LV +VGLS LQ+T   S RN+ IVGVSLFLGLSIP YF+ +  +++               GP HT
Sbjct:  213 VVERFALIIALIIVWAYAHVLTASGAYKH-----------------RPHQ-TQLNCRTDMSNLISSAPWIKIPYPLQWGAPSFDAGHAFAMMAAVLV---------------------------------------------------SLIESTGAFKAAARLASATPPPPHVLSRGIGWQGIGILLNGLFGTLSGSSVSVENIGLLGSTRVGSRRVIQISAGFMIFFSMLGKFGALFASIPFTIFAAVYCVLFGLVASVGLSFLQFTNMNSLRNLFIVGVSLFLGLSIPEYFRDFSMKAL--------------HGPAHT 450          

HSP 2 Score: 120.553 bits (301), Expect = 7.278e-27
Identity = 64/172 (37.21%), Postives = 103/172 (59.88%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHI--MRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            L+  +  NP  G  I  G +H++   G+ + IPS++VP MGG D D   V+ T+L + G++T+L + FG+RLP V G S+ ++ P + II+      + + + R +  MR +QGAIIV S  Q ILGFS + ++  R  +P+ + P +A  G   F+ GFP  G+C+E+ +P
Sbjct:   20 LEYCIDSNPPWGEAIALGFEHYILALGTAVMIPSILVPMMGGDDGDKVRVVQTLLFLQGVNTLLQTLFGTRLPTVIGGSYAFMVPIISIIHDSSLTRIEDPQLRFLSTMRAVQGAIIVASSVQIILGFSQMWAICSRFFSPIGMVPVIALTGFGLFNRGFPVVGNCVEIGLP 191          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT7_ARATH (Nucleobase-ascorbate transporter 7 OS=Arabidopsis thaliana GN=NAT7 PE=2 SV=2)

HSP 1 Score: 149.058 bits (375), Expect = 6.400e-36
Identity = 108/325 (33.23%), Postives = 142/325 (43.69%), Query Frame = 1

Query: 2485 GRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHT 3459
            G   +     V  SV I WIYA  LT GGAY   G +                  T   CR D S     + W+R+PYP QWG P FH   +  M+ VS V                                                   ++ +  GTY   S   S+ PP P V+SRG+  +G   LL G++G+G G++   EN   + +T++ SRR +   A F+I FS++GK GAI ASIP  + AA+ C  +A V A GLS LQ+    SFR   I+G S+F+GLSIP YF QY               A    GPVHT
Sbjct:  221 GERQVFHRFAVIFSVVIVWIYAHLLTVGGAYKNTGVN------------------TQTSCRTDRSGLISGSPWIRVPYPFQWGPPTFHAGEAFAMMAVSFV---------------------------------------------------SLIESTGTYIVVSRFASATPPPPSVLSRGVGWQGVGVLLCGLFGAGNGASVSVENAGLLALTRVGSRRVVQISAGFMIFFSILGKFGAIFASIPAPVVAALHCLFFAYVGAGGLSLLQFCNLNSFRTKFILGFSVFMGLSIPQYFNQYT--------------AVNKYGPVHT 462          

HSP 2 Score: 129.028 bits (323), Expect = 1.658e-29
Identity = 68/158 (43.04%), Postives = 104/158 (65.82%), Query Frame = 3

Query: 1113 IYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLT--EHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            I  G QH+L + G+ + IP+ +VP MGG +++ A ++ T+L VSG++T+L S+FG+RLP V G S+ Y+   L II A  Y ++   + KF+ IMR +QGA+IV SI Q ++GFSGL   ++RL++P+   P VA  G   + +GFP    CIE+ +P
Sbjct:   46 ILLGFQHYLVMLGTTVLIPTYLVPQMGGGNEEKAKMVQTLLFVSGLNTLLQSFFGTRLPAVIGGSYTYVPTTLSIILAGRYSDILDPQEKFKRIMRGIQGALIVASILQIVVGFSGLWRNVVRLLSPLSAVPLVALAGFGLYEHGFPLLAKCIEIGLP 203          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT4_ARATH (Nucleobase-ascorbate transporter 4 OS=Arabidopsis thaliana GN=NAT4 PE=2 SV=2)

HSP 1 Score: 140.198 bits (352), Expect = 4.670e-33
Identity = 103/302 (34.11%), Postives = 140/302 (46.36%), Query Frame = 1

Query: 2479 FVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQY 3384
            F  +  I     V  ++ I W YA  LTA GAYD                  ++   T   CR D S     + WVRIPYPLQWG P FH   +  M+  + VA                                                   I +  G++ +AS   S+    P V+SRGI  +G   LL G++G+ TGST L ENT  + +TK+ SRR +   A F+I FS+ GK GA+LASIPL + AA+ C ++A V + GL  LQ+    SFRN  I+G S+F+GLS+  YF +Y
Sbjct:  207 FKCKRSICEQFAVLFTIAIVWAYAEILTAAGAYD------------------KRPDNTQLSCRTDRSGLISASPWVRIPYPLQWGRPSFHGSDAFAMMAATYVA---------------------------------------------------IVETTGSFIAASRFGSATHIPPSVLSRGIGWQGIGVLLNGLFGTATGSTALVENTGLLGLTKVGSRRVVQISAGFMIFFSIFGKFGAVLASIPLPIFAALYCVLFAYVASAGLGLLQFCNLNSFRNKFILGFSIFIGLSVAQYFTEY 439          

HSP 2 Score: 126.716 bits (317), Expect = 8.267e-29
Identity = 71/154 (46.10%), Postives = 100/154 (64.94%), Query Frame = 3

Query: 1113 IYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVI-INARDYRNLTEH-KFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIE 1568
            I  G QH++ + G+ + IPS++VP MGG D + A VI+T+L VSGI+T+L S FGSRLP+V G+S+ YL PAL I  + R    L  H +F   MR +QGA+I+ SI   I+GF GL  +L+R ++P+  AP V   G+   ++ FPQ   CIE
Sbjct:   34 IVLGFQHYIVMLGTTVIIPSILVPLMGGGDVEKAEVINTVLFVSGINTLLQSLFGSRLPVVMGASYAYLIPALYITFSYRFTYYLHPHLRFEETMRAIQGALIIASISHMIMGFFGLWRILVRFLSPLSAAPLVILTGVGLLAFAFPQLARCIE 187          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT6_ARATH (Nucleobase-ascorbate transporter 6 OS=Arabidopsis thaliana GN=NAT6 PE=2 SV=2)

HSP 1 Score: 139.043 bits (349), Expect = 9.513e-33
Identity = 107/325 (32.92%), Postives = 148/325 (45.54%), Query Frame = 1

Query: 2485 GRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHT 3459
            G++V  R   V  +V I WIYA  LT GGAY+  G +P                 T   CR D +     A W+R+P+P QWG P F    +  M++ S VA V+S   +                                               V  Y SA++L       P ++SRGI  +G   L++G++G+G GS+   EN   + +T++ SRR +   A F+I FS++GK GA+ ASIP  + AA+ C  +A V A GLS LQ+    SFR   I+G S+FLGLSIP YF +Y               A    GPVHT
Sbjct:  216 GKNVFDR-FAVIFAVVIVWIYAHLLTVGGAYN--GAAPT----------------TQTSCRTDRAGIIGAAPWIRVPWPFQWGAPSFDAGEAFAMMMASFVALVESTGAFVA---------------------------------------------VSRYASATML------PPSILSRGIGWQGVAILISGLFGTGAGSSVSVENAGLLALTRVGSRRVVQIAAGFMIFFSILGKFGAVFASIPAPIIAALYCLFFAYVGAGGLSFLQFCNLNSFRTKFILGFSVFLGLSIPQYFNEYT--------------AIKGYGPVHT 456          

HSP 2 Score: 118.627 bits (296), Expect = 3.584e-26
Identity = 64/158 (40.51%), Postives = 98/158 (62.03%), Query Frame = 3

Query: 1113 IYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTE--HKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            I  G QH+L + G+ + IP+ +VP MGG  ++ A VI T+L V+GI+T+L + FG+RLP V G+S+ ++   + II +  + + +    +F  IMR  QGA+IV S  Q ILGFSGL   ++R ++P+   P V  VG   + +GFP    CIE+ +P
Sbjct:   40 ILLGFQHYLVMLGTTVLIPTALVPQMGGGYEEKAKVIQTILFVAGINTLLQTLFGTRLPAVVGASYTFVPTTISIILSGRFSDTSNPIDRFERIMRATQGALIVASTLQMILGFSGLWRNVVRFLSPISAVPLVGLVGFGLYEFGFPGVAKCIEIGLP 197          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT3_ARATH (Nucleobase-ascorbate transporter 3 OS=Arabidopsis thaliana GN=NAT3 PE=2 SV=2)

HSP 1 Score: 128.642 bits (322), Expect = 2.188e-29
Identity = 95/283 (33.57%), Postives = 129/283 (45.58%), Query Frame = 1

Query: 2527 VTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYF 3375
            + I W +A  LT  GAY+    +                  T + CR D +    +A W+RIPYP QWG PIF       M   ++VAS +S                                                    G + +AS L  +  P   VVSR I L+G   LL GI+GS TG+T   EN   + +T++ SRR +     F+I FS+ GK GA  ASIPL + A + C +  +VVAVG+S +Q+T T S RN+ ++GVSLFL LSI  YF
Sbjct:  247 LAIIWAFAAILTVSGAYNNVSTA------------------TKQSCRTDRAFLMSSAPWIRIPYPFQWGTPIFKASHVFGMFGAAIVASAEST---------------------------------------------------GVFFAASRLAGATAPPAHVVSRSIGLQGIGVLLEGIFGSITGNTASVENVGLLGLTRIGSRRVVQVSTFFMIFFSIFGKFGAFFASIPLPIFAGVYCILLGIVVAVGISFIQFTDTNSMRNMYVIGVSLFLSLSIAQYF 460          

HSP 2 Score: 110.153 bits (274), Expect = 1.997e-23
Identity = 65/174 (37.36%), Postives = 100/174 (57.47%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRN----LTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            L+  +  NP     +    QH++ + G+ + I + +V  MGG   D A VI T+L +SGI+T+L +  G+RLP V G SF Y+ P L II  RDY N      + +FRH MR +QG++I+ S    I+G+      L+R+ +P++V P V+ V L  F  GFP   +C+E+ +P
Sbjct:   44 LQYCIHSNPSWHETVVLAFQHYIVMLGTTVLIANTLVSPMGGDPGDKARVIQTILFMSGINTLLQTLIGTRLPTVMGVSFAYVLPVLSII--RDYNNGQFDSEKQRFRHTMRTVQGSLIISSFVNIIIGYGQAWGNLIRIFSPIIVVPVVSVVSLGLFLRGFPLLANCVEIGLP 215          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy Swiss-Prot
Match: NAT8_ARATH (Nucleobase-ascorbate transporter 8 OS=Arabidopsis thaliana GN=NAT8 PE=2 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 3.459e-29
Identity = 94/289 (32.53%), Postives = 135/289 (46.71%), Query Frame = 1

Query: 2521 LSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWG-VPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQY 3384
             SV I W+YA+ LT GGAY           SN  ++       T   CR D +     + W+R+P+P+QWG  P F+      M+  S V                                                   ++ +  GTY + S   S+ P  P V+SRGI  +GF  LL G++G+G  ++   EN   + +T++ SRR +   A F+I FS++GK GAI ASIP  + AA+ C  ++ V A GLS +Q+    SFR   I+G S+F+GLSIP YF QY
Sbjct:  233 FSVVIVWLYAYILTIGGAY-----------SNTEIN-------TQISCRTDRAGIISASPWIRVPHPIQWGGAPTFNAGDIFAMMAASFV---------------------------------------------------SLVESTGTYIAVSRYASATPIPPSVLSRGIGWQGFGILLCGLFGAGNATSVSVENAGLLAVTRVGSRRVIQVAAGFMIFFSILGKFGAIFASIPAPIVAALYCLFFSYVGAGGLSLIQFCNLNSFRTKFILGFSIFMGLSIPQYFYQY 452          

HSP 2 Score: 112.079 bits (279), Expect = 4.819e-24
Identity = 60/168 (35.71%), Postives = 97/168 (57.74%), Query Frame = 3

Query: 1083 LRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLT--EHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVP 1580
            L   P     I  G QH+L + G+ + IP+++V  +   ++D   +I T+L VSGI+T+  S+FG+RLP V G+S+ Y+   + I+ A  Y ++   + +F  IMR +QGA+I+ S    ++GFSGL   + R ++P+   P VA  G   +  GFP    CIE+ +P
Sbjct:   34 LTSPPPWPETILLGFQHYLVMLGTTVLIPTMLVSKIDARNEDKVKLIQTLLFVSGINTLFQSFFGTRLPAVIGASYSYVPTTMSIVLAARYNDIMDPQKRFEQIMRGIQGALIIASFLHILVGFSGLWRNVTRFLSPLSAVPLVAFSGFGLYEQGFPMLAKCIEIGLP 201          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A0D2T9J8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G058700 PE=4 SV=1)

HSP 1 Score: 603.208 bits (1554), Expect = 0.000e+0
Identity = 405/417 (97.12%), Postives = 409/417 (98.08%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDXXXXXXXXXXXKTVADGNGLGEKVEERKXXXXXXXXXXXXXXXXXXXXASVTPLXXXXXXXXXXXXXXXXIGMYPXXXXXXXXXXXXXXXXLKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV-RTLTYHLVYTF 1697
            METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDSGSGSGSRQGRKTVADGNGLGEKVEERKVGLNGNGVENGNGNGKGNGVASVTPLVVEEKDEEGKGGRDVEIGMYPEGEEQGHEGGNGHHPGLKVGLRDNPG GALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFG+RLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLR INPVVVAPTVAAVGLAFFSYGFPQAGSCIEVSVPLLLLVLICTLYLRGISIFGHRLFRIYAV  ++T   +Y F
Sbjct:    1 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDSGSGSGSRQGRKTVADGNGLGEKVEERKVGLNGNGVENGNGNGKGNGVASVTPLVVEEKDEEGKGGRDVEIGMYPEGEEQGHEGGNGHHPGLKVGLRDNPGFGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGTRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRWINPVVVAPTVAAVGLAFFSYGFPQAGSCIEVSVPLLLLVLICTLYLRGISIFGHRLFRIYAVPLSVTITWIYAF 417          

HSP 2 Score: 506.523 bits (1303), Expect = 1.041e-157
Identity = 274/341 (80.35%), Postives = 280/341 (82.11%), Query Frame = 1

Query: 2455 SLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            +L+L GI   G H + R   VPLSVTITWIYAFFLTAGGAYDYKGCSP+IPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDS                                                  QVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFS+VGKVGAILASIPLSLAAAILCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQ+ 
Sbjct:  386 TLYLRGISIFG-HRLFRIYAVPLSVTITWIYAFFLTAGGAYDYKGCSPDIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDS--------------------------------------------------QVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSIVGKVGAILASIPLSLAAAILCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQLD 675          

HSP 3 Score: 108.997 bits (271), Expect = 1.012e-20
Identity = 62/62 (100.00%), Postives = 62/62 (100.00%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGKF 3846
            QLDFSINALLSMSMVVTLLVAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGKF
Sbjct:  673 QLDFSINALLSMSMVVTLLVAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGKF 734          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A0D2S7N0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G058700 PE=4 SV=1)

HSP 1 Score: 603.208 bits (1554), Expect = 0.000e+0
Identity = 405/417 (97.12%), Postives = 409/417 (98.08%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDXXXXXXXXXXXKTVADGNGLGEKVEERKXXXXXXXXXXXXXXXXXXXXASVTPLXXXXXXXXXXXXXXXXIGMYPXXXXXXXXXXXXXXXXLKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV-RTLTYHLVYTF 1697
            METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDSGSGSGSRQGRKTVADGNGLGEKVEERKVGLNGNGVENGNGNGKGNGVASVTPLVVEEKDEEGKGGRDVEIGMYPEGEEQGHEGGNGHHPGLKVGLRDNPG GALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFG+RLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLR INPVVVAPTVAAVGLAFFSYGFPQAGSCIEVSVPLLLLVLICTLYLRGISIFGHRLFRIYAV  ++T   +Y F
Sbjct:    1 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDSGSGSGSRQGRKTVADGNGLGEKVEERKVGLNGNGVENGNGNGKGNGVASVTPLVVEEKDEEGKGGRDVEIGMYPEGEEQGHEGGNGHHPGLKVGLRDNPGFGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGTRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRWINPVVVAPTVAAVGLAFFSYGFPQAGSCIEVSVPLLLLVLICTLYLRGISIFGHRLFRIYAVPLSVTITWIYAF 417          

HSP 2 Score: 502.286 bits (1292), Expect = 3.485e-156
Identity = 273/341 (80.06%), Postives = 279/341 (81.82%), Query Frame = 1

Query: 2455 SLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            +L+L GI   G H + R   VPLSVTITWIYAFFLTAGGAYDYKGCSP+IPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDS                                                   VGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFS+VGKVGAILASIPLSLAAAILCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQ+ 
Sbjct:  386 TLYLRGISIFG-HRLFRIYAVPLSVTITWIYAFFLTAGGAYDYKGCSPDIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDS---------------------------------------------------VGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSIVGKVGAILASIPLSLAAAILCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQLD 674          

HSP 3 Score: 108.612 bits (270), Expect = 1.285e-20
Identity = 62/62 (100.00%), Postives = 62/62 (100.00%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGKF 3846
            QLDFSINALLSMSMVVTLLVAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGKF
Sbjct:  672 QLDFSINALLSMSMVVTLLVAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGKF 733          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A0B0P656_GOSAR (Nucleobase-ascorbate transporter 11-like protein OS=Gossypium arboreum GN=F383_02598 PE=4 SV=1)

HSP 1 Score: 551.206 bits (1419), Expect = 1.783e-179
Identity = 362/390 (92.82%), Postives = 365/390 (93.59%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDXXXXXXXXXXXKTVADGNGLGEKVEERKXXXXXXXXXXXXXXXXXXXXASVTPLXXXXXXXXXXXXXXXXIGMYPXXXXXXXXXXXXXXXXLKVGLRDNPGLG-----------------ALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIE 1568
            METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPK+DQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKS RDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDSGSGSGSRQGRKTVADGNGLGEKVEERKVGLNGNGVENGNG G G   ASVTPLVVEEKDEEGKGGRDVEIGMYPEGEEQGHEGGNGHHPGLKVGLRDNPG G                 ALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTA+VISTMLLVSGISTILHSYFG+RLPLVQGSSFIYLAPALVIINARDYRNL EHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINPVVVAPTVAAVGLAFFSYGFPQAGSCIE
Sbjct:    1 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKRDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSSRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDSGSGSGSRQGRKTVADGNGLGEKVEERKVGLNGNGVENGNGKGNGV--ASVTPLVVEEKDEEGKGGRDVEIGMYPEGEEQGHEGGNGHHPGLKVGLRDNPGFGRNQYTRPVLIRLSLPTGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTATVISTMLLVSGISTILHSYFGTRLPLVQGSSFIYLAPALVIINARDYRNLAEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINPVVVAPTVAAVGLAFFSYGFPQAGSCIE 388          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A0B0N5M4_GOSAR (Nucleobase-ascorbate transporter 11 OS=Gossypium arboreum GN=F383_11654 PE=4 SV=1)

HSP 1 Score: 533.102 bits (1372), Expect = 2.245e-173
Identity = 287/342 (83.92%), Postives = 288/342 (84.21%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQ 3471
            MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHA+TMKHCR DASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDS                                                   VGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTIN TKMASRRALVFGALFLILFSVVGKVGAILASIPLSLAAAILCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIP YFQQYQPESILMLPSYFVPYAAASDGPVHTINEQ
Sbjct:    1 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAFTMKHCRTDASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDS---------------------------------------------------VGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINKTKMASRRALVFGALFLILFSVVGKVGAILASIPLSLAAAILCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPAYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQ 291          

HSP 2 Score: 116.316 bits (290), Expect = 4.507e-24
Identity = 53/55 (96.36%), Postives = 53/55 (96.36%), Query Frame = 2

Query: 3734 TTQFQAAGTNEGCTFGHVLKMSQLIHPCNWITLCQENFPHFVVVLAVCGHNPQQK 3898
            TTQFQAAG NEGCTFGHVLKMSQLIHPCNWITLCQENFPHFVVVLAVCGHN QQK
Sbjct:  296 TTQFQAAGKNEGCTFGHVLKMSQLIHPCNWITLCQENFPHFVVVLAVCGHNTQQK 350          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A061DHL0_THECC (Xanthine/uracil permease family protein isoform 3 OS=Theobroma cacao GN=TCM_000977 PE=4 SV=1)

HSP 1 Score: 500.36 bits (1287), Expect = 1.692e-157
Identity = 334/427 (78.22%), Postives = 364/427 (85.25%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDXXXXXXXXXXXKTVA----DGNGLGEKVEERKXXXXXXXXXXXXXXXXXXXXAS------VTPLXXXXXXXXXXXXXXXXIGMYPXXXXXXXXXXXXXXXXLKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV-RTLTYHLVYTF 1697
            MET S S+SL+KAE S GRGNKLGSMLPKIEPFVPK+D  HNPRELRSWAKRTGFVSHLSGETGTASST       GFH  + GRDQRAGGSSPKIEIDPVLGRT+PN G+EIE DSGS SGS  G+   A    +GNGLG+KV+ER+ GLNGN    GNGN       +      VTP++ E+K  EGKG RDVE+GMYP GE+QGH G +GHHPG+KVGLRDNPG GALIYYGLQHFLSLAGSLIFIP VMVPAMGGTDKDTA+VISTMLLVSGI+TILHSYFG+RLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAII+GS+FQSILGFSGLMSLLLRLINPVVVAPTVAAVGLAFFSYGFP+AGSC+EVSVPL+LLVLICTLYLRGISIFGHR FRIYAV  ++    +Y F
Sbjct:    1 METRS-SNSLEKAEPSRGRGNKLGSMLPKIEPFVPKRD--HNPRELRSWAKRTGFVSHLSGETGTASST-------GFHAERGGRDQRAGGSSPKIEIDPVLGRTKPNRGIEIEADSGSRSGSGSGQGRRAVPDENGNGLGDKVDEREAGLNGNAKGIGNGNLHVVENGNGNGVPGVTPVISEQKHGEGKGERDVEVGMYPGGEQQGHGGWSGHHPGMKVGLRDNPGFGALIYYGLQHFLSLAGSLIFIPLVMVPAMGGTDKDTATVISTMLLVSGITTILHSYFGTRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIIGSVFQSILGFSGLMSLLLRLINPVVVAPTVAAVGLAFFSYGFPRAGSCVEVSVPLILLVLICTLYLRGISIFGHRFFRIYAVPLSVMITWIYAF 417          

HSP 2 Score: 322.398 bits (825), Expect = 1.579e-92
Identity = 170/251 (67.73%), Postives = 179/251 (71.31%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKV 3198
            ++ +L+L GI   G H   R   VPLSV ITWIYAFFLT GGAYDYKGCSP+IPSSNILVDEC+KHAYTMKHCR D SNAWR AAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSV  Y                                                   HSASLLVSSKPPTP VVSRGIALEGFCS+LAG+WGSGTGSTTLTENTHTINITKMASRRA+VFGALFLILFS VGKV
Sbjct:  383 LICTLYLRGISIFG-HRFFRIYAVPLSVMITWIYAFFLTTGGAYDYKGCSPDIPSSNILVDECKKHAYTMKHCRTDVSNAWRNAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVGTY---------------------------------------------------HSASLLVSSKPPTPAVVSRGIALEGFCSMLAGLWGSGTGSTTLTENTHTINITKMASRRAVVFGALFLILFSFVGKV 581          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A061DHG4_THECC (Transducin/WD40 repeat-like superfamily protein isoform 2 OS=Theobroma cacao GN=TCM_000977 PE=4 SV=1)

HSP 1 Score: 501.13 bits (1289), Expect = 8.688e-157
Identity = 334/427 (78.22%), Postives = 364/427 (85.25%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDXXXXXXXXXXXKTVA----DGNGLGEKVEERKXXXXXXXXXXXXXXXXXXXXAS------VTPLXXXXXXXXXXXXXXXXIGMYPXXXXXXXXXXXXXXXXLKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV-RTLTYHLVYTF 1697
            MET S S+SL+KAE S GRGNKLGSMLPKIEPFVPK+D  HNPRELRSWAKRTGFVSHLSGETGTASST       GFH  + GRDQRAGGSSPKIEIDPVLGRT+PN G+EIE DSGS SGS  G+   A    +GNGLG+KV+ER+ GLNGN    GNGN       +      VTP++ E+K  EGKG RDVE+GMYP GE+QGH G +GHHPG+KVGLRDNPG GALIYYGLQHFLSLAGSLIFIP VMVPAMGGTDKDTA+VISTMLLVSGI+TILHSYFG+RLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAII+GS+FQSILGFSGLMSLLLRLINPVVVAPTVAAVGLAFFSYGFP+AGSC+EVSVPL+LLVLICTLYLRGISIFGHR FRIYAV  ++    +Y F
Sbjct:    1 METRS-SNSLEKAEPSRGRGNKLGSMLPKIEPFVPKRD--HNPRELRSWAKRTGFVSHLSGETGTASST-------GFHAERGGRDQRAGGSSPKIEIDPVLGRTKPNRGIEIEADSGSRSGSGSGQGRRAVPDENGNGLGDKVDEREAGLNGNAKGIGNGNLHVVENGNGNGVPGVTPVISEQKHGEGKGERDVEVGMYPGGEQQGHGGWSGHHPGMKVGLRDNPGFGALIYYGLQHFLSLAGSLIFIPLVMVPAMGGTDKDTATVISTMLLVSGITTILHSYFGTRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIIGSVFQSILGFSGLMSLLLRLINPVVVAPTVAAVGLAFFSYGFPRAGSCVEVSVPLILLVLICTLYLRGISIFGHRFFRIYAVPLSVMITWIYAF 417          

HSP 2 Score: 317.005 bits (811), Expect = 6.493e-90
Identity = 167/248 (67.34%), Postives = 176/248 (70.97%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVV 3189
            ++ +L+L GI   G H   R   VPLSV ITWIYAFFLT GGAYDYKGCSP+IPSSNILVDEC+KHAYTMKHCR D SNAWR AAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSV  Y                                                   HSASLLVSSKPPTP VVSRGIALEGFCS+LAG+WGSGTGSTTLTENTHTINITKMASRRA+VFGALFLILFS V
Sbjct:  383 LICTLYLRGISIFG-HRFFRIYAVPLSVMITWIYAFFLTTGGAYDYKGCSPDIPSSNILVDECKKHAYTMKHCRTDVSNAWRNAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVGTY---------------------------------------------------HSASLLVSSKPPTPAVVSRGIALEGFCSMLAGLWGSGTGSTTLTENTHTINITKMASRRAVVFGALFLILFSFV 578          

HSP 3 Score: 87.4261 bits (215), Expect = 4.492e-14
Identity = 53/62 (85.48%), Postives = 57/62 (91.94%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGKF 3846
            QLDF+INAL+SMSMVVTLLVAFVLDNTVPGS+ ERGVYIWS AEDV+ DPS Q DYSLPGKF
Sbjct:  591 QLDFTINALMSMSMVVTLLVAFVLDNTVPGSQQERGVYIWSRAEDVSMDPSSQADYSLPGKF 652          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A061DPP1_THECC (Xanthine/uracil permease family protein isoform 1 OS=Theobroma cacao GN=TCM_000977 PE=4 SV=1)

HSP 1 Score: 501.516 bits (1290), Expect = 5.872e-156
Identity = 334/427 (78.22%), Postives = 364/427 (85.25%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRGNKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMDXXXXXXXXXXXKTVA----DGNGLGEKVEERKXXXXXXXXXXXXXXXXXXXXAS------VTPLXXXXXXXXXXXXXXXXIGMYPXXXXXXXXXXXXXXXXLKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV-RTLTYHLVYTF 1697
            MET S S+SL+KAE S GRGNKLGSMLPKIEPFVPK+D  HNPRELRSWAKRTGFVSHLSGETGTASST       GFH  + GRDQRAGGSSPKIEIDPVLGRT+PN G+EIE DSGS SGS  G+   A    +GNGLG+KV+ER+ GLNGN    GNGN       +      VTP++ E+K  EGKG RDVE+GMYP GE+QGH G +GHHPG+KVGLRDNPG GALIYYGLQHFLSLAGSLIFIP VMVPAMGGTDKDTA+VISTMLLVSGI+TILHSYFG+RLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAII+GS+FQSILGFSGLMSLLLRLINPVVVAPTVAAVGLAFFSYGFP+AGSC+EVSVPL+LLVLICTLYLRGISIFGHR FRIYAV  ++    +Y F
Sbjct:    1 METRS-SNSLEKAEPSRGRGNKLGSMLPKIEPFVPKRD--HNPRELRSWAKRTGFVSHLSGETGTASST-------GFHAERGGRDQRAGGSSPKIEIDPVLGRTKPNRGIEIEADSGSRSGSGSGQGRRAVPDENGNGLGDKVDEREAGLNGNAKGIGNGNLHVVENGNGNGVPGVTPVISEQKHGEGKGERDVEVGMYPGGEQQGHGGWSGHHPGMKVGLRDNPGFGALIYYGLQHFLSLAGSLIFIPLVMVPAMGGTDKDTATVISTMLLVSGITTILHSYFGTRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIIGSVFQSILGFSGLMSLLLRLINPVVVAPTVAAVGLAFFSYGFPRAGSCVEVSVPLILLVLICTLYLRGISIFGHRFFRIYAVPLSVMITWIYAF 417          

HSP 2 Score: 464.537 bits (1194), Expect = 3.706e-142
Identity = 253/344 (73.55%), Postives = 267/344 (77.62%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            ++ +L+L GI   G H   R   VPLSV ITWIYAFFLT GGAYDYKGCSP+IPSSNILVDEC+KHAYTMKHCR D SNAWR AAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDS                                                   VGTYHSASLLVSSKPPTP VVSRGIALEGFCS+LAG+WGSGTGSTTLTENTHTINITKMASRRA+VFGALFLILFS V KVGAILASIPLSLAA+ILCFMWALVVA+GLSTLQYTQT SFRNITIVGVSLFLGLSIP YFQQYQPES L+LPSYFVPYAAASDGPV T N+Q+ 
Sbjct:  383 LICTLYLRGISIFG-HRFFRIYAVPLSVMITWIYAFFLTTGGAYDYKGCSPDIPSSNILVDECKKHAYTMKHCRTDVSNAWRNAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDS---------------------------------------------------VGTYHSASLLVSSKPPTPAVVSRGIALEGFCSMLAGLWGSGTGSTTLTENTHTINITKMASRRAVVFGALFLILFSFVAKVGAILASIPLSLAASILCFMWALVVALGLSTLQYTQTTSFRNITIVGVSLFLGLSIPAYFQQYQPESSLILPSYFVPYAAASDGPVRTGNKQLD 674          

HSP 3 Score: 89.7373 bits (221), Expect = 8.550e-15
Identity = 53/62 (85.48%), Postives = 57/62 (91.94%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGKF 3846
            QLDF+INAL+SMSMVVTLLVAFVLDNTVPGS+ ERGVYIWS AEDV+ DPS Q DYSLPGKF
Sbjct:  672 QLDFTINALMSMSMVVTLLVAFVLDNTVPGSQQERGVYIWSRAEDVSMDPSSQADYSLPGKF 733          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A067K0Y3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18707 PE=4 SV=1)

HSP 1 Score: 437.187 bits (1123), Expect = 9.655e-132
Identity = 235/342 (68.71%), Postives = 262/342 (76.61%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQ 3471
            +V SL+L G+   G H + R   VPLSV I W YAFFLTAGGAY+YKGCSP+IPSSNILVD CR+HAYTM+HCR D SNA RT+AWVRIPYPLQWG+PIFH RTSLIMIIVSLVASVDS                                                   VGTYHS S LV+SKPPTPG+VSRGIALEGFCSLLAG+WGSGTGSTTLTEN HTINITK+ASRRA+V GA+FLILFS VGKVGAILASIPL+LAA++LCFMW L+VA+GLSTLQY+QTASFRNI IVGVSLFLGLSIP YFQQYQPES L+LPSYFVP+AAAS+GPVHT ++Q
Sbjct:  403 LVFSLYLRGLSIFG-HRLFRVYAVPLSVMIIWTYAFFLTAGGAYNYKGCSPDIPSSNILVDACRRHAYTMQHCRTDVSNALRTSAWVRIPYPLQWGIPIFHLRTSLIMIIVSLVASVDS---------------------------------------------------VGTYHSTSFLVNSKPPTPGIVSRGIALEGFCSLLAGLWGSGTGSTTLTENVHTINITKVASRRAVVLGAVFLILFSFVGKVGAILASIPLALAASVLCFMWGLIVALGLSTLQYSQTASFRNIAIVGVSLFLGLSIPAYFQQYQPESSLILPSYFVPFAAASNGPVHTSSKQ 692          

HSP 2 Score: 308.145 bits (788), Expect = 7.482e-86
Identity = 171/209 (81.82%), Postives = 198/209 (94.74%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAVRTLTYHLVYTF 1697
            +K GLRDNPG   L+YYGLQH+LSLAGSLIFIP ++VPAMGGTDKDTA+VISTMLL+SGI+TILHSYFG+RLPLVQGSSFIYLAPALVI+NAR+YRNL+E+KFRHIMRELQGAIIVGSIFQ ILGF+GLMSLLLRLINPVVVAPTVAAVGLAFFSYGFPQAGSC+E+S+PL+LLVL+ +LYLRG+SIFGHRLFR+YAV  L+  +++T+
Sbjct:  228 MKFGLRDNPGFVPLMYYGLQHYLSLAGSLIFIPLIIVPAMGGTDKDTATVISTMLLISGITTILHSYFGTRLPLVQGSSFIYLAPALVIMNAREYRNLSENKFRHIMRELQGAIIVGSIFQVILGFTGLMSLLLRLINPVVVAPTVAAVGLAFFSYGFPQAGSCVEISIPLILLVLVFSLYLRGLSIFGHRLFRVYAV-PLSVMIIWTY 435          

HSP 3 Score: 124.405 bits (311), Expect = 1.505e-25
Identity = 74/119 (62.18%), Postives = 85/119 (71.43%), Query Frame = 3

Query:  450 METGSNSDSLDKAETSNGRG---NKLGSMLPKIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFHVGKSGRDQRAGGSSPKIEIDPVLGRTRPNMGVEIEMD 797
            METGS+S+ +DK+ T   R      LGSMLPKIEPFV + D  HNPRELRSWAKRTGF+S  S ET T S++ +FDSSTGF + K     + GGSSPKIEIDPVLGRTR   G EIE D
Sbjct:    1 METGSSSNPVDKSGTQRERAKTNTNLGSMLPKIEPFVARTD--HNPRELRSWAKRTGFISTFSSET-TTSNSGKFDSSTGFDLEKGVHHHKNGGSSPKIEIDPVLGRTRTARGSEIEAD 116          

HSP 4 Score: 82.4185 bits (202), Expect = 1.549e-12
Identity = 45/61 (73.77%), Postives = 52/61 (85.25%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            Q DF+INAL+S++MVVT L+AFVLDNTVPG+R ERGVYIWS  ED+ TDPSL  DYSLP K
Sbjct:  692 QFDFAINALMSLNMVVTFLIAFVLDNTVPGTRQERGVYIWSHPEDLVTDPSLHADYSLPQK 752          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: A0A067EZH2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g013981mg PE=4 SV=1)

HSP 1 Score: 423.705 bits (1088), Expect = 5.005e-131
Identity = 228/359 (63.51%), Postives = 264/359 (73.54%), Query Frame = 1

Query: 2401 LPQFILLNNFGLSRKMVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQVS 3477
            +PQ +L+        ++ +L+L GI   G H + R   VPLSV I W YAFFLTAGGAY+YKGCSP+IPSSNIL D C KHAYTMKHCR D SNAWRTAAWVRIPYPLQWG+PIFH RTSLIMIIVSLVASVDS                                                   VGTYH+ASLLV+SKPPTP +VSRGI +EGFCS+LAG+WGSGTGS+TLTEN HTI+ITK+ASRRA+  GA+F+I+FS VGKVGAILASIP +LAA+ILCFMWAL+VA+GLS LQYTQ+ASFRNI IVG SLFLGLSIP YFQQY PE+ L+LPSYFVP+AAAS+GPVHT ++Q+ 
Sbjct:   63 IPQILLV--------LIFALYLRGISVFGHH-LFRIYAVPLSVMIIWAYAFFLTAGGAYNYKGCSPDIPSSNILSDACIKHAYTMKHCRTDVSNAWRTAAWVRIPYPLQWGIPIFHLRTSLIMIIVSLVASVDS---------------------------------------------------VGTYHTASLLVNSKPPTPRIVSRGIGMEGFCSILAGLWGSGTGSSTLTENVHTISITKVASRRAVQLGAVFMIVFSFVGKVGAILASIPQALAASILCFMWALIVAIGLSNLQYTQSASFRNIMIVGASLFLGLSIPAYFQQYHPETSLILPSYFVPFAAASNGPVHTGSKQLD 361          

HSP 2 Score: 124.79 bits (312), Expect = 1.730e-26
Identity = 80/92 (86.96%), Postives = 87/92 (94.57%), Query Frame = 3

Query: 1389 MRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAV 1664
            MRELQGAIIVGSIFQSILGF+GLMSL LRLINPVVVAPTVAA+GLAFFSYGFPQAGSC+E+S+P +LLVLI  LYLRGIS+FGH LFRIYAV
Sbjct:    1 MRELQGAIIVGSIFQSILGFTGLMSLFLRLINPVVVAPTVAAIGLAFFSYGFPQAGSCVEISIPQILLVLIFALYLRGISVFGHHLFRIYAV 92          

HSP 3 Score: 90.8929 bits (224), Expect = 1.092e-15
Identity = 51/61 (83.61%), Postives = 57/61 (93.44%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            QLDF+INAL+SM+MVVTLLVAF+LDNTVPGSR ERGVYIWS AED+A+DPSLQ DYSLP K
Sbjct:  359 QLDFAINALMSMNMVVTLLVAFILDNTVPGSRQERGVYIWSRAEDLASDPSLQADYSLPRK 419          

BLAST of g.barbadense_cottongen_reftransV1_0094223 vs. ExPASy TrEMBL
Match: B9HFM4_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s14230g PE=4 SV=2)

HSP 1 Score: 433.335 bits (1113), Expect = 1.438e-130
Identity = 232/342 (67.84%), Postives = 260/342 (76.02%), Query Frame = 1

Query: 2446 MVDSLFLVGIFFVGRHVIVRSLQVPLSVTITWIYAFFLTAGGAYDYKGCSPEIPSSNILVDECRKHAYTMKHCRADASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDSVSIYCVPKYRIKWTV*WKYFI*HLISVNLRH*TRNKDNGPYIDACDTITKQVGTYHSASLLVSSKPPTPGVVSRGIALEGFCSLLAGIWGSGTGSTTLTENTHTINITKMASRRALVFGALFLILFSVVGKVGXXXXXXXXXXXXXXXCFMWALVVAVGLSTLQYTQTASFRNITIVGVSLFLGLSIPTYFQQYQPESILMLPSYFVPYAAASDGPVHTINEQ 3471
            ++ +L+L GI   G H I +   VPLSV + W YAFFLTAGGAY+YKGCSP++PSSNILVD CRKHAYTM+HCR DASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDS                                                   VGTYHS SLLV+SKPPTP +VSRGIALEGFCS+LAGIWG GTGSTTLTEN HT+NITK+ASRR +  GA FLILFS +GKVGAILASIP +LAA+ILCFMW L+V++GLSTLQY+QTASFRNITIVGVSLFLGL+IP YFQQYQPES L+LPSYFVPYAAAS+GPV T ++Q
Sbjct:  375 LIFTLYLRGISIFG-HRIFQIYAVPLSVLMIWTYAFFLTAGGAYNYKGCSPDVPSSNILVDACRKHAYTMQHCRTDASNAWRTAAWVRIPYPLQWGVPIFHFRTSLIMIIVSLVASVDS---------------------------------------------------VGTYHSTSLLVNSKPPTPRIVSRGIALEGFCSVLAGIWGCGTGSTTLTENVHTVNITKVASRRVVEVGAAFLILFSFIGKVGAILASIPQALAASILCFMWGLIVSLGLSTLQYSQTASFRNITIVGVSLFLGLTIPAYFQQYQPESSLILPSYFVPYAAASNGPVQTSSKQ 664          

HSP 2 Score: 308.531 bits (789), Expect = 2.800e-86
Identity = 170/209 (81.34%), Postives = 197/209 (94.26%), Query Frame = 3

Query: 1071 LKVGLRDNPGLGALIYYGLQHFLSLAGSLIFIPSVMVPAMGGTDKDTASVISTMLLVSGISTILHSYFGSRLPLVQGSSFIYLAPALVIINARDYRNLTEHKFRHIMRELQGAIIVGSIFQSILGFSGLMSLLLRLINXXXXXXXXXXXGLAFFSYGFPQAGSCIEVSVPXXXXXXXXXXXXRGISIFGHRLFRIYAVRTLTYHLVYTF 1697
            ++ GLRDNPG   L+YYGLQH+LS+AGSLIFIP ++VPAMGGTD+DTA VISTMLL+SGI+TILHSYFG+RLPLVQGSSF+YLAPALVIINAR+YRNLTEHKFRHIMRELQGAIIVGS+FQ+ILGF+G MSLLLRLINPVVVAPTVAAVGLAFFSYGFPQAGSC+E+S+PL+LLVLI TLYLRGISIFGHR+F+IYAV  L+  +++T+
Sbjct:  200 MRYGLRDNPGFALLMYYGLQHYLSMAGSLIFIPLIIVPAMGGTDRDTAEVISTMLLISGITTILHSYFGTRLPLVQGSSFVYLAPALVIINAREYRNLTEHKFRHIMRELQGAIIVGSLFQTILGFTGFMSLLLRLINPVVVAPTVAAVGLAFFSYGFPQAGSCVEISIPLILLVLIFTLYLRGISIFGHRIFQIYAV-PLSVLMIWTY 407          

HSP 3 Score: 82.8037 bits (203), Expect = 1.319e-12
Identity = 55/119 (46.22%), Postives = 70/119 (58.82%), Query Frame = 3

Query:  534 KIEPFVPKKDQNHNPRELRSWAKRTGFVSHLSGETGTASSTERFDSSTGFH--VGKSGRDQRAGGSSPKIEIDPVLGRTRP-NMGVEIEMDXXXXXXXXXXXKTVADGNGLGEKVEERK 881
            KI+PFVP+ D  HNPRELRSWAKRTGFVS  S ET T++ T     ++  +     +    R GGSSPKIEIDP+LGRTR  N  +EIE +           +   D  GLG + E ++
Sbjct:   33 KIDPFVPRTD--HNPRELRSWAKRTGFVSTFSSETTTSNDTATPTPASELYNKAVDNNNHHRNGGSSPKIEIDPILGRTRQLNSRIEIEPE----------SRPGNDDRGLGLRDESKR 139          

HSP 4 Score: 82.0333 bits (201), Expect = 2.339e-12
Identity = 47/61 (77.05%), Postives = 54/61 (88.52%), Query Frame = 1

Query: 3661 QLDFSINAXXXXXXXXXXXXAFVLDNTVPGSRDERGVYIWSCAEDVATDPSLQLDYSLPGK 3843
            Q DF++NAL+S++MVVTLLVAFVLDNTVPG+R ERGVYIWS AED+ATD SL  DYSLP K
Sbjct:  664 QFDFAMNALMSLNMVVTLLVAFVLDNTVPGNRQERGVYIWSRAEDMATDTSLHADYSLPSK 724