Mutant Texas marker-1 (TM-1) cotton plants containing the Li 2 gene were crossed with the Upland cotton variety DP5690 and F 1 progeny were backcrossed for five generations (BC 5) by SSD to DP5690 which served as the recurrent parent in each backcross. The DP5690 recurrent parent was a pure inbred line that was self- pollinated for nine generations via SSD. Progeny in each backcross were selected based on phenotype for the Li 2 short-fiber mutation.