Gossypium hirsutum (AD1) ZM24 genome CRI Assembly v1 & Annotation a1
About the assembly
The ZM24 genome assembly was conducted using a Canu-based pipeline (Koren, S. et al. 2017) with the following procedures: longer seed reads were selected with the settings genomeSize = 600000000 and corOutCoverage = 35; raw reads overlapping was detected through a high sensitive overlapper MHAP (mhap-2.1.2, option corMhapSensitivity = low/normal/high), and an error correction was performed through Falcon sense method (option correctedErrorRate = 0.025); error-corrected reads were trimmed of unsupported bases and hairpin adapters in order to reach their longest supporting range with the default parameters, and then the draft assembly was generated using the top 80% longest trimmed reads. The raw Illumina reads were filtered as mentioned in the Illumina sequencing section. Finally, the clean reads from the same sequencing individuals were integrated to correct the SNPs and InDels in the draft assemblies using Pilon v1.22 (Walker, B. J. et al., 2014) with the parameters -mindepth 10 –changes –threads 4 –fix bases.
The chromosomes (pseudomolecules) for G. hrisutum ZM24 genome. These files belong to the CRI Assembly v1
The predicted gene model, their alignments and proteins for G. hirsutum 'TM-1' genome. These files belong to the CRI-ZM24 Assembly v1 & Annotation a1.0
All annotation files are available for download by selecting the desired data type in the left-hand "Resources" side bar. Each data type page will provide a description of the available files and links do download. Alternatively, you can use the FTP repository for bulk download.
Homology of the Gossypium hirsutum CRI-ZM24 v1.0 proteins was determined by pairwise sequence comparison using the blastp algorithm against various protein databases. An expectation value cutoff less than 1e-9 was used for the NCBI nr (Release 2018-05) and 1e-6 for the Arabidoposis proteins (TAIR10), UniProtKB/SwissProt (Release 2019-01), and UniProtKB/TrEMBL (Release 2019-01) databases. The best hit reports are available for download in Excel format.
Transcript alignments were performed by the CottonGen Team of Main Bioinformatics Lab at WSU. The alignment tool 'BLAT' was used to map transcripts to the G. hirsutum genome assembly. Alignments with an alignment length of 97% and 90% identify were preserved. The available files are in GFF3 format.
Marker alignments were performed by the CottonGen Team of Main Bioinformatics Lab at WSU. The alignment tool 'BLAT' was used to map marker sequences from CottonGen to the Gossypium hirsutum genome assembly. Markers required 90% identity over 97% of their length. For SSRs & RFLPs, gap size was restricted to 1000bp or less with less than 2 gaps. For dbSNPs and Indels gap size was restricted to 2bp with less than 2 gaps. The available files are in GFF3 format. Markers available in CottonGen are linked to JBrowse.
Functional annotation files for the Gossypium hirsutum CRI-ZM24 Genome v1.0 are available for download below. The Gossypium hirsutum CRI-ZM24 Genome proteins were analyzed using InterProScan in order to assign InterPro domains and Gene Ontology (GO) terms. Pathways analysis was performed using the KEGG Automatic Annotation Server (KAAS).