Simple Sequence Repeat (SSR) Genetic Linkage Map of D Genome Diploid Cotton Derived from an Interspecific Cross between Gossypium davidsonii and Gossypium klotzschianum

Publication Overview
TitleSimple Sequence Repeat (SSR) Genetic Linkage Map of D Genome Diploid Cotton Derived from an Interspecific Cross between Gossypium davidsonii and Gossypium klotzschianum
AuthorsKirungu JN, Deng Y, Cai X, Magwanga RO, Zhou Z, Wang X, Wang Y, Zhang Z, Wang K, Liu F
TypeJournal Article
Journal NameInternational journal of molecular sciences
Volume19
Issue1
Year2018
CitationKirungu JN, Deng Y, Cai X, Magwanga RO, Zhou Z, Wang X, Wang Y, Zhang Z, Wang K, Liu F. Simple Sequence Repeat (SSR) Genetic Linkage Map of D Genome Diploid Cotton Derived from an Interspecific Cross between Gossypium davidsonii and Gossypium klotzschianum. International journal of molecular sciences. 2018 Jan 11; 19(1).

Abstract

The challenge in tetraploid cotton cultivars is the narrow genetic base and therefore, the bottleneck is how to obtain interspecific hybrids and introduce the germplasm directly from wild cotton to elite cultivars. Construction of genetic maps has provided insight into understanding the genome structure, interrelationships between organisms in relation to evolution, and discovery of genes that carry important agronomic traits in plants. In this study, we generated an interspecific hybrid between two wild diploid cottons, Gossypium davidsonii and Gossypium klotzschianum, and genotyped 188 F2:3 populations in order to develop a genetic map. We screened 12,560 SWU Simple Sequence Repeat (SSR) primers and obtained 1000 polymorphic markers which accounted for only 8%. A total of 928 polymorphic primers were successfully scored and only 728 were effectively linked across the 13 chromosomes, but with an asymmetrical distribution. The map length was 1480.23 cM, with an average length of 2.182 cM between adjacent markers. A high percentage of the markers on the map developed, and for the physical map of G. raimondii, exhibited highly significant collinearity, with two types of duplication. High level of segregation distortion was observed. A total of 27 key genes were identified with diverse roles in plant hormone signaling, development, and defense reactions. The achievement of developing the F2:3 population and its genetic map constructions may be a landmark in establishing a new tool for the genetic improvement of cultivars from wild plants in cotton. Our map had an increased recombination length compared to other maps developed from other D genome cotton species.

Featuremaps
This publication contains information about 2 maps:
Map Name
D3K-57 x D3D-1, F2:3 (2018)
D3K-57 x D3D-1, F2:3-P (2018)
Stocks
This publication contains information about 1 stocks:
Stock NameUniquenameType
cdbpop_000144D3K-57 x D3D-1, F2:3population
Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic
ISSN1422-0067
eISSN1422-0067
Publication Date2018 Jan 11
Journal AbbreviationInt J Mol Sci
PIIE204
Elocation10.3390/ijms19010204
DOI10.3390/ijms19010204
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountrySwitzerland
Cross References
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DatabaseAccession
PMID: PubMedPMID:29324636