Development of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes

Overview
TitleDevelopment of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes
AuthorsZhai C, Xu P, Zhang X, Guo Q, Zhang X, Xu Z, Shen X
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
VolumeN/A
IssueN/A
Year2015
Page(s)N/A
CitationZhai C, Xu P, Zhang X, Guo Q, Zhang X, Xu Z, Shen X. Development of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2015 May 9.

Abstract

KEY MESSAGE
We reported the first development of Gossypium anomalum -derived microsatellite markers and identification of recombination between sexually incompatible species by a synthesized hexaploid on genome level. To continue to develop improved cotton varieties, it is essential to transfer desired characters from diploid wild cotton species such as Gossypium anomalum to cultivated allotetraploid cotton species. However, interspecific reproductive barriers limit gene transfer between species. In a previous study, we used colchicine treatment to produce a synthesized hexaploid derived from an interspecific hybrid between Gossypium hirsutum and G. anomalum and demonstrated its hybridity and doubled status using morphological, cytological and molecular marker methods. In the current study, to effectively monitor G. anomalum genome components in the G. hirsutum background, we developed 5974 non-redundant G. anomalum-derived SSR primer pairs using RNA-Seq technology, which were combined with a publicly available physical map. Based on this combined map and segregation data from the BC2F1 population, we identified a set of 230 informative G. anomalum-specific SSR markers distributed on the chromosomes, which cover 95.72 % of the cotton genome. After analyzing BC2F1 segregation data, 50 recombination types from 357 recombination events were identified, which cover 81.48 % of the corresponding G. anomalum genome. A total of 203 recombination events occurred on chromosome 11, accounting for 56.86 % of the recombination events on all chromosomes. Recombination hotspots were observed at marker intervals JAAS1148-NAU5100 on chromosome 1 and JAAS0426-NAU998 on chromosome 2. Therefore, all G. anomalum chromosomes are capable of recombining with At chromosomes in G. hirsutum. This study represents an important step towards introgressing desirable traits into cultivated cotton from the wild cotton species G. anomalum.

Featuremaps
This publication contains information about 1 maps:
Map Name
(86-1 x G. anomalum) x Su8289, BC2F1 (2015)
Features
This publication contains information about 6,654 features:
Feature NameUniquenameType
JAAS0001JAAS0001genetic_marker
JAAS0002JAAS0002genetic_marker
JAAS0003JAAS0003genetic_marker
JAAS0004JAAS0004genetic_marker
JAAS0005JAAS0005genetic_marker
JAAS0006JAAS0006genetic_marker
JAAS0007JAAS0007genetic_marker
JAAS0008JAAS0008genetic_marker
JAAS0009JAAS0009genetic_marker
JAAS0010JAAS0010genetic_marker
JAAS0011JAAS0011genetic_marker
JAAS0012JAAS0012genetic_marker
JAAS0013JAAS0013genetic_marker
JAAS0014JAAS0014genetic_marker
JAAS0015JAAS0015genetic_marker
JAAS0016JAAS0016genetic_marker
JAAS0017JAAS0017genetic_marker
JAAS0018JAAS0018genetic_marker
JAAS0019JAAS0019genetic_marker
JAAS0020JAAS0020genetic_marker
JAAS0021JAAS0021genetic_marker
JAAS0022JAAS0022genetic_marker
JAAS0023JAAS0023genetic_marker
JAAS0024JAAS0024genetic_marker
JAAS0025JAAS0025genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1432-2242
eISSN1432-2242
Publication Date2015 May 9
Journal AbbreviationTheor. Appl. Genet.
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:25957115