The Li2 Mutation Results in Reduced Subgenome Expression Bias in Elongating Fibers of Allotetraploid Cotton (Gossypium hirsutum L.)

Publication Overview
TitleThe Li2 Mutation Results in Reduced Subgenome Expression Bias in Elongating Fibers of Allotetraploid Cotton (Gossypium hirsutum L.)
AuthorsNaoumkina M, Thyssen G, Fang DD, Hinchliffe DJ, Florane C, Yeater KM, Page JT, Udall JA
TypeJournal Article
Journal NamePloS one
Volume9
Issue3
Year2014
Page(s)e90830
CitationNaoumkina M, Thyssen G, Fang DD, Hinchliffe DJ, Florane C, Yeater KM, Page JT, Udall JA. The Li2 Mutation Results in Reduced Subgenome Expression Bias in Elongating Fibers of Allotetraploid Cotton (Gossypium hirsutum L.). PloS one. 2014; 9(3):e90830.

Abstract

Next generation sequencing (RNA-seq) technology was used to evaluate the effects of the Ligon lintless-2 (Li2) short fiber mutation on transcriptomes of both subgenomes of allotetraploid cotton (Gossypium hirsutum L.) as compared to its near-isogenic wild type. Sequencing was performed on 4 libraries from developing fibers of Li2 mutant and wild type near-isogenic lines at the peak of elongation followed by mapping and PolyCat categorization of RNA-seq data to the reference D5 genome (G. raimondii) for homeologous gene expression analysis. The majority of homeologous genes, 83.6% according to the reference genome, were expressed during fiber elongation. Our results revealed: 1) approximately two times more genes were induced in the AT subgenome comparing to the DT subgenome in wild type and mutant fiber; 2) the subgenome expression bias was significantly reduced in the Li2 fiber transcriptome; 3) Li2 had a significantly greater effect on the DT than on the AT subgenome. Transcriptional regulators and cell wall homeologous genes significantly affected by the Li2 mutation were reviewed in detail. This is the first report to explore the effects of a single mutation on homeologous gene expression in allotetraploid cotton. These results provide deeper insights into the evolution of allotetraploid cotton gene expression and cotton fiber development.

Properties
Additional details for this publication include:
Property NameValue
Journal AbbreviationPLoS ONE
Journal CountryUnited States
LanguageEnglish
Language Abbreng
Publication Date2014
Publication ModelElectronic-eCollection
Publication TypeJournal Article
URLhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090830
DOI10.1371/journal.pone.0090830
eISSN1932-6203
Elocation10.1371/journal.pone.0090830
ISSN1932-6203
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:24598808