Comparative analysis of 3D genome organization in cotton reveals the topological basis for transcriptional divergence during polyploidization

Working group session: 
Comparative Genomics and Bioinformatics
Presentation type: 
15 minute Oral
Maojun Wang, Pengcheng Wang, Min Lin, Guoliang Li, Qingyong Yang, and Xianlong Zhang*
Author Affliation: 
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.
The spatial partition of the genome into higher-order structures underpins pivotal regulatory functions, but knowledge of three-dimensional (3D) genome structure and its dynamics during polyploidization remains poor. Here, we characterize 3D genome architectures for diploid and tetraploid cotton, and found the existence of A/B compartments and topologically associated domains (TADs). By comparing chromatin interaction maps in cotton, this study interrogates the reorganization of chromatin during polyploidization. We also identified inter-subgenomic interactions and linked these interactions to expression of homoeologous genes. This study offers evolutionary insights into 3D genome organization and transcriptional regulation in polyploids. Keywords: Cotton; 3D genome; Hi-C data analysis; transcriptional regulation; polyploidization