Working group session:
Germplasm and Genetic Stocks
Presentation type:
poster
Authors:
Hou, Meiying; Zhou, Baoliang; Hou, Meiying; Zhou, Baoliang
Presenter:
Hou, Meiying; Hou, Meiying
Correspondent:
Zhou, Baoliang; Zhou, Baoliang
Abstract:
Gossypium hirsutum L. (Upland cotton), an allotetraploid with a base chromosome number of 13 (2n = 4x = 52) and a genome size of 2500 Mbp, is the major cash crop in China. In the present study, ninety-three F2 individuals derived from the interspecific cross, G. hirsutum L. acc 08N2162 and G. tomentosum Nutt. ex Seem., were used to construct the genetic map (HT map) with simple sequence repeats (gSSR) and simple sequence repeats derived from expressed sequence tags (EST–SSR). 1045 loci were detected from 807 pairs of EST–SSR, and 755 loci from 540 pairs of gSSR. Polymorphisms from gSSR and EST-SSR was very similar, 16.0 % (540 in 3380) and 15.8% (807 in 5180), respectively. A total of 1800 loci from 1347 pairs of primers were detected, of which there were 682 co-dominant markers, 458 dominant for upland cotton and 660 for G. tomentosum. Of the 1800 loci, 1295 loci were grouped into 46 linkage groups at LOD≧4, and the map covered 3,375.3 cM with a mean density of 2.86 cM per locus. All the above linkage groups have been located on their corresponding chromosomes using the genetic map of interspecific cross between TM-1 (G. hirsutum L.) and Hai 7124 (G. barbadense L.) (HB map) as reference. The same reciprocal translocations were found between A4 and A5, A2 and A3 chromosomes on both genetic maps. There were 506 (28.1%) markers showed segregation distortion, of which 255 (14.1%) markers located and 24 SDRs totally were detected. The distribution of distorted marker loci were very uneven between At subgenome and Dt, less distorted marker loci were located into At (89) than D sub-genome (166), 7 SDRs appeared in the At and 17 SDRs appeared in the Dt. Five distorted markers located on 12 short linkage groups.