Working group session:
Functional Genomics
Presentation type:
oral
Authors:
Halepoto, Abdul Rehman; Halepoto, Abdul Rehman
Presenter:
Halepoto, Abdul Rehman; Halepoto, Abdul Rehman
Correspondent:
Halepoto, Abdul Rehman; Halepoto, Abdul Rehman
Abstract:
ABSTRACT: Genetic diversity/phylogenetic study in six cotton cultivars were carried out realeased by Central Cotton Research Institute Sakrand. The study was carried out by using RAPD markers. In this study 15 universal RAPD primers were used. The RAPD profiles obtained were successfully used to differentiate the genotypes. Based on the pair-wise comparison of amplification products, the genetic diversity/similarity was estimated. The four cotton cultivars showed variation at the DNA level. The genetic similarity among all cotton cultivars ranged from 70 to 85%. CRIS-467 was quite distant from CRIS-134, CRIS-121, CRIS-342 and CRIS-5A (Marvi) cultivars. A dendrogram was constructed using NTSYSpc programme. On the basis of this analysis, the populations were clustered into two clusters: cluster I contained CRIS-121 and CRIS-134 cultivars and cluster II contained CRIS-467, CRIS-342, CRIS-5A and CRIS-9 cultivars. Therefore, the polymorphism detected and its reproducibility suggest that RAPD markers are reliable for identification of cotton cultivars.