Working group session:
Breeding and Applied Genomics
Presentation type:
oral
Authors:
Romanel, Elisson; Lee, Tae-Ho; Paterson, Andrew; Vaslin, Maite ; Romanel, Elisson; Lee, Tae-Ho; Paterson, Andrew; Vaslin, Maite
Presenter:
Romanel, Elisson; Romanel, Elisson
Correspondent:
Vaslin, Maite ; Vaslin, Maite
Abstract:
Small RNA biogenesis regulatory network evolution in Gossypium raimondii Elisson Romanel1, Tae-Ho Lee2, Andrew Paterson2 and Maite F.S. Vaslin3 1Lab. Biologia Evolutiva Teórica e Aplicada, Depto. Genética, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil; 2Plant Genome Mapping Laboratory, The University of Georgia, Athens, GA, USA; 3Lab. Virologia Molecular Vegetal (LVMV), I. Microbiologia, UFRJ, Rio de Janeiro, Brazil. The genus Gossypium is composed by ~50 species ranging from 885 Mb per haploid nucleus in D-genome (G.raimondii) to 2572 Mb in K-genome. Plant small RNA biogenesis pathway, key component of RNA-silencing mechanism, are essential for eukaryote development, nutritional and stress responses, chromatin regulation and viral defense. Searching for Dicer-like (DCL), Argonaute (AGO), RNA-dependent RNA polymerase (RDR) and nuclear RNA polymerase IV/V (PolIV/V) gene sequence in G. raimondii we surprisingly find duplication gene event for DCL3, AGO1, AGO4, AGO7, AGO10, RDR1 and Pol IVa. Beyond these extra DCL3, AGO1, AGO4, AGO10 and Pol IVa-2 proteins, G. raimondii genome showed a higher number of isoforms of these genes compared to the others plants species studied here. All these paralogous cotton duplicated genes showed a KS value which fit with the unique cotton whole genome duplication event. It is the first report of an extra-DCL3 and Pol IVa protein existence in eudicots. Most of these genes (DLC3, AGO4 and Pol IVa) are involved in 24-nt siRNA production and secondary steps DNA methylation and chromatin remodeling at their target loci. The increase number of genes and its isoforms points out a possible role of them in the control the large amount of retrotransposons and repetitive DNA elements present in cotton species.