Working group session:
Germplasm and Genetic Stocks
Presentation type:
poster
Authors:
Ye, Wuwei
Presenter:
Ye, Wuwei
Correspondent:
Ye, Wuwei
Abstract:
Salinity resistance and differential gene expression associated with salinity in cotton germplasm were studied, according to the large scale of saline area in China and its negative significant effects on the cotton production. The salinity-resisted genes and their differential expression were studied under the stress of NaCl on cotton. There found, under the NaCl stress, 1644 genes differentially expressed from the salinity-sensitive cotton and only 817genes differentially expressed from the salinity-resisted cotton. The differential expressed genes could be divided into five groups according to the expressed levels by the cluster analysis.
Two genes were excavated by gene chips and SSH librarys, cloned by RACE and RT-PCR, named GhPTAC and GhGnT separately. Salt-resistant related expression analysis studied for the two genes by Real-time PCR. More in-depth researches for GhGnT gene were studied by GFP sub-cellular localization and yeast expression technology. The full length cDNA sequences of GhPTAC and GhGnT were obtained by RACE technology combined with RT-PCR. The full length of GhPTAC is 1564bp, including 5'-UTR 41bp, complete ORF 1038bp and 3'-UTR 485bp, which encodes a polypeptide of 345 amino acids. The full length of GhGnT is 2140bp, including 5'-UTR 587bp, complete ORF 1353bp, 3'-UTR 200bp, and PolyA 27bp, which encodes a polypeptide of 450 amino acids.