Working group session:
Structural Genomics
Presentation type:
oral
Authors:
Fang, David; Jenkins, Johnie ; McCarty, Jack; Deng, Dewayne
Presenter:
Fang, David
Correspondent:
Fang, David
Abstract:
The objective of this research is to identify molecular markers associated with quantitative trait loci controlling fiber quality traits based on the genome wide association study (GWAS). Five hundred fifty recombinant inbred lines (RILs) derived after 6 cycles of random mating of 11 parental varieties were previously developed at USDA-ARS, Mississippi State. A total of 730 polymorphic simple sequence repeat (SSR) markers were selected based on their mapped positions. An average of 28 markers was chosen from each chromosome. These markers were analyzed among 11 parental varieties and 275 RILs, and revealed 1264 loci. Of them, 849 are polymorphic loci that cover all 26 chromosomes, and the average marker locus interval is about 3 cM. The 550 RILs were planted in Starkville, Mississippi between 2009 and 2011 to obtain fiber quality data. Marker-trait associations were conducted using JMP Genomics and TASSELL software packages. Markers that were identified as associated with fiber quality QTLs were tested in the other 275 RILs to confirm the associations. Detail marker-fiber quality QTL associations will be presented in the conference.