Mapping quantitative trait loci associated with cotton leaf curl virus (clcuv) and flower characteristics in cotton (Gossypium l.) using microsatellite markers

Working group session: 
Breeding and Applied Genomics
Presentation type: 
poster
Authors: 
Muhammad, Khan; Muhammad, Khan; Muhammad, Khan; Muhammad, Khan
Presenter: 
Muhammad, Khan; Muhammad, Khan; Muhammad, Khan; Muhammad, Khan
Correspondent: 
Muhammad, Khan; Muhammad, Khan
Abstract: 
Genetic dissection of the virus resistance/tolerance varieties may assist in developing Cotton Leaf Curl Disease (CLCuD) tolerant upland cotton. The present study was conducted to identify and map quantitative trait loci (QTLs) conferring CLCuD resistance and cotton flower traits in an interspecific cross (Gossypium barbadense x Gossypium anomaulum) x Gossypium hirsutum population. An F2 population was developed which differed greatly in striking characteristics. A genetic map covering 395 cM, with 89 marker loci and 7 linkage groups, was constructed by using this F2 population. The F2 population was grown in field and flower traits (Pollen colour, petal colour, petal basal red spot, bracket size and stem hairiness) or disease tolerance were evaluated. QTL analysis via composite interval mapping detected 2 QTLs for the disease tolerance and 3 QTLs for flower traits in F2 population were identified. Two significant QTLs for cotton leaf curl virus (qCLCVa1 & qCLCVa2), were located on chromosomes 9 at 25 cM and 22.1 cM respectively and each explains 18% of PV in F2 population. When both of these QTLs were fitted simultaneously, they explained 36% of the phenotypic variance. The peak LOD scores were about 3.36 and 3.26 respectively. One QTL for pollen colour was identified on the Chromosoe 5 with accumulative phenotypic variance of 0.13%. One significant QTL for Petal Colour was found on chromosomes 11 (9.5 cM) with less additive effect from dominant parent. The peak LOD score was about 3.6. Its contribution toward phenotypic variance was 0.2 %. A QTL for Red Petal Spot was detected on chromosome 4 (9 cM) with LOD score value of 3.29. Individually the QTLs accounted for 0.2-18% of phenotypic variation. The QTLs for cotton leaf curl disease tolerance, firstly detected in Gossypium, may provide a basis for marker-assisted selection to improve productivity in cotton-crop breeding.