Working group session:
Germplasm and Genetic Stocks
Presentation type:
poster
Authors:
Imran, Muhammad; Liu, Jin-Yuan
Presenter:
Imran, Muhammad
Correspondent:
Liu, Jin-Yuan
Abstract:
Malate dehydrogenase (MDH) is an enzyme of the citric acid cycle that catalyzes the reversible oxidation of malate to oxaloacetate with the concomitant reduction of NADH and plays crucial roles in various cellular processes. Here, we identified 12 malate dehydrogenase (MDH) genes from diploid cotton species Gossypium raimondii, and designated GrMDH1-12. These MDH members were located on 10 chromosomes in G. raimondii with uneven distribution. Their exon-intron numbers and genomic positions were highly conserved in different plant species. A phylogenetic analysis showed that MDH putative proteins can be divided into four groups, MDH I, MDH II, MDH III and MDH IV that contain 2, 3, 3 and 4 members, respectively. Expression analysis indicates that 11 GrMDH genes, except GrMDH 10, are differentially expressed in various cotton fiber, seeds and vegetative tissues. More importantly, eight of 12 GrMDH genes showed higher expression level in the fibers, particularly in early fiber elongation stage, demonstrating the high potential roles of MDH in the cotton fiber cells elongation. Finally, a promoter element analysis of the GrMDH genes indicates redundant but distinctive cis-regulatory elements for fiber cell development and stress responsiveness. This study is the first to characterize MDH gene family in cotton and provides a foundation for future studies on specific functions of these genes in fiber development.