Working group session:
Functional Genomics
Presentation type:
poster
Authors:
Afolabi-Balogun, Nusrah; Inuwa, Hajiya
Presenter:
Afolabi-Balogun, Nusrah
Correspondent:
Afolabi-Balogun, Nusrah
Abstract:
Genetic transformation method using Agrobacterium tumefaciens was developed for cotton plant species. Following the efficient regeneration of three cotton varieties, the effect of inclusion of acetosyringone in co-cultivation medium was measured. Subsequently, transformation was obtained by co- cultivation of 3 weeks old cotton apical shoot and A. tumefaciens strain LBA 4404 containing a plasmid harboring neomycin phosphotransferase and b-glucuronidase encoding genes. PCR analyses performed to identify the presence of marker gene (npt II) in the transgenic plants using primers used for amplification of a 700 bp fragment of the npt II gene showed that 87% of the transgenic plants selected for kanamycin resistance were transformed with the gene encoding b-glucuronidase. Routine transformation efficiency of cotton was established at 1.3%. The mean number of GUS positive apices was 67% higher when acetosyringone was included in the medium.