Working group session:
Germplasm and Genetic Stocks
Presentation type:
poster
Authors:
Ernazarova, Dilrabo; Sanamyan, Marina; Rizaeva, Safiya; Abdullaev, Abdumavlyan; Kushanov, Fakhriddin
Presenter:
Correspondent:
Ernazarova, Dilrabo; Kushanov, Fakhriddin
Abstract:
Interspecific cotton hybrids are included both the theoretical and practical interest for systematics and evolution of the genius Gossypium L. There are problems of the distant hybridization experiences such as crossability of parental species and sterility of F1 hybrids. The causes of these unfavorable phenomena can be revealed through the cytological method. In thise work, microsporogenesis of F1 hybrids between representatives of different species - G.hirsutum L. (AD1) and G.barbadense L. (AD2) has been examined. Cytological results pointed out that F1 hybrids G.hirsutum L. x G.barbadense L. involved minor alterations in M1 of meiosis.
In F1 hybrids, G.hirsutum L. x G.hirsutum L. microsporogenesis takes its normal with extremely rare anomalous microspores. The pollen fertility of the hybrid plants were 90,9 and 95,5%, whereas in other hybrids (G.hirsutum ssp.punctatum var.gambia x G.hirsutum ssp.punctatum, G.hirsutum ssp.punctatum var.gambia x G.hirsutum ssp.purpurascens) the pollen fertility was shown 82,2 and 83,2%.
In first generation of hybrids G.hirsutum L. x G.barbadense L. microsporogenesis occurred normally as intraspecific crosses. Anomalous microspores were identified extremely rare in interspecific hybridization. The meiotic index and pollen fertility were consisted to 90,7 and 94,9%, respectively, whereas in reciprocal hybrids (G.barbadense ssp.darwinii х G.hirsutum ssp.purpurascens var.el-salvador and G.hirsutum ssp.purpurascens var.el-salvador x G.barbadense ssp.darwinii) the meiotic index was shown as 87,6 and 86,7%, respectively.
Cytological studies of microsporogenesis indicated phylogenetic relationship between species G.hirsutum L. and G.barbadense L. The quadrivalent chromosome associations at the M1 meiosis were detected among hybrids G.barbadense ssp.darwinii х G.hirsutum ssp.purpurascens var.el-salvador and G.barbadense ssp.vitifolium f.brasiliense х G.hirsutum ssp.mexicanum var.nervosum (Jucatan)). Thus, cytological studies confirm the regular character of meiosis in the tetraploid hybrids.