Working group session:
Functional Genomics
Presentation type:
poster
Authors:
liu, guoyuan; nan, wenzhi; wu, man; yu, jiwen; pei, wenfeng; yu, shuxun
Presenter:
Correspondent:
yu, shuxun
Abstract:
Background: MicroRNAs, a kind of non-coding small RNAs, playing an improtant role in regulating gene expression during post-transcriptional stage in plant. To identify differentially expressed miRNAs related to differences in fiber length between two near-isogenic lines（NILs） that will facilitate the uncovering of the mechanism of fiber elongation, a high-throughput sequencing technology was applied.
Result: Two libraries were constructed during elongation stage between the two NILs. A total of 35 known miRNAs families and 169 novel miRNAs were found by aligning to the cotton D genomic and the miRBase (version 18.0). A number of 16 known miRNAs and 60 novel miRNAs were found to have significant difference between the two NILs. Soon a bioinformatics and a degradome were taken to analyze the targets of miRNAs. A total of 236 targets were found, with obvious function related to plant hormone pathways and RIG-I-like receptor signaling pathways, such as auxin, brassinosteroid and so on. Then a real-time fluorescence quantitative PCR with some chosen miRNAs and targets were taken to measure the relationship among miRNAs, targets, and the length of fiber.
Conclusion: This study shows the function of miRNAs during fiber elongation by regulating their targets. And some novel miRNAs in allopolyploid cotton were found. We also revealed a possible pathway of some miRNAs influencing the fiber length, which deserved further study.
Key words: Allopolyploid cotton, fiber elongation, miRNA, high-throughput sequence