Gene expression analysis in Upland cotton under Cotton Fleahopper (Miridae: Pseudatomoscelis seriatus) feeding stress

Working group session: 
Germplasm and Genetic Stocks
Presentation type: 
poster
Authors: 
McLoud, Laura Ann; Hague, Steven; Jones, Don
Presenter: 
McLoud, Laura Ann
Correspondent: 
McLoud, Laura Ann; Hague, Steven
Abstract: 
Cotton fleahopper (Pseudatomoscelis seriatus) (Hemiptera: Miridae) is a piercing-sucking insect that has emerged as a major pest in the Texas cotton industry over the past decade. Cotton fleahopper feeding results in square damage, abscission, and subsequent yield-loss. Previous studies in Upland cotton (Gossypium hirsutum) indicate that plant trichome density plays an important role in conferring resistance to cotton fleahopper, but the mechanism of resistance remains largely unknown. In this project, an RNA sequencing experiment was performed to measure gene expression in four Upland cotton genotypes on which the cotton fleahopper had fed and on which the insect had not fed. Genotypes were selected for testing based on previous data regarding their level of susceptibility or resistance to cotton fleahopper feeding damage. RNA sequencing was performed using the Illumina HiSeq 2000 platform, and Gossypium raimondii was used as the reference genome.