Working group session:
Germplasm and Genetic Stocks
Presentation type:
poster
Authors:
Makamov, Abdusalom ; Usmonov, Dilshod; Darmanov, Mukhtor; Tulanov, Akmal; Turaev, Ozod; Kushanov, Fakhriddin; Shapulatov, Umidjon; Ayubov, Mirzakamol; Soliev, Azamjon; Shermatov, Shukhrat; Buriev, Zabardast; Abdukarimov, Abdusattor; Saha, Sukumar; Jenkins, Johnie; Stelly, David; Abdurakhmonov, Ibrokhim
Presenter:
Makamov, Abdusalom
Correspondent:
Makamov, Abdusalom ; Abdurakhmonov, Ibrokhim
Abstract:
Chromosome substitution backcrossing lines (CS-B) have a pair of chromosomes or chromosome arms from Pima cotton (Gossypium barbadense) which replaced the respective pair of chromosomes or chromosome arms of TM-1 (G. hirsutum). These lines are largely similar to each other, except that each differs by the replacement of a specific homologous pair of chromosomes from Pima 3-79 into Upland genetic background (Texas Marker-1). The aim of this research is to develop chromosome specific recombinant inbred mapping populations using CS-B lines for future performance of fine association mapping of agronomically important QTLs. Development of chromosome specific mapping population based on a set of CS-B lines are of importance to enrich genetic diversity in cotton because it will show wide segregation in a single substituted chromosome from Pima cotton. In this study, the commercial Uzbek cotton cultivar AN-Boyovut-2 was used as a common parent and 17 CS-B lines as donor genotypes. The common parent, An-Boyovut-2 is an elite cultivar, having high yield and salt tolerance but middle fiber quality, was crossed with 17 CS-B donor lines in 2006, followed by self-pollination to develop 17 segregating F2 populations. In order to get the next generations of these populations, 11 segregating F2-3 populations were chosen in which the existence of substituted chromosomes were confirmed by chromosome specific DNA markers. The created chromosome specific recombinant inbred mapping populations will be excellent genetic resource for dissecting and fine mapping superior fiber quality loci.