Identification of candidate genes and development of functional markers for Root-Knot Nematode resistance loci in Upland cotton

Working group session: 
Structural Genomics
Presentation type: 
poster
Authors: 
Kumar, Pawan; He, Yajun; Shen, Xinlian; Davis, Richard; Nichols, Robert; Jones, Don; Chee, Peng
Presenter: 
Chee, Peng
Correspondent: 
Chee, Peng
Abstract: 
Host plant resistance in the line Auburn 623RNR and a number of elite breeding lines derived from it exhibit the highest level of resistance to root-knot nematode (Meloidogyne incognita; RKN) know to date in Upland cotton (Gossypium hirsutum L.) Identification and characterization of the resistant genes in this resistant source is of great interest to cotton nematologists and breeders. Genetic mapping analysis has identified two QTLs, qMi-C11 and qMi-C14, conferring resistance to RKN in the Auburn 623RNR source. The QTL qMi-C11 is identified on an 810 Kb region flanked by markers CIR316 and CIR069 on Chr.11 while the QTL qMi-C14 is identified on a 2.3 Mb region flanked by markers BNL3644 and CGR5668 on Chr.14. Primary objectives of this study are to identify potential candidate genes for RKN resistance on chr.11 and chr.14 based on gene annotations and to study sequence variation between RKN resistance and susceptible lines for the identified candidate genes leading to the development of functional SNP markers for efficient marker assisted selection.