Working group session:
Functional Genomics
Presentation type:
poster
Authors:
Khan, Muhammad Daud; Zhu, ShuiJin
Presenter:
Khan, Muhammad Daud
Correspondent:
Zhu, ShuiJin
Abstract:
Due to its toxic nature, cadmium (Cd) causes ultramorphological, metabolic, and proteomic changes in plants. We designed the present study to study Cd stress (0, 500 µM) in upland cotton cultivar (ZMS-49) using physiological, metabolic and proteomic biomarkers. One way ANOVA was performed along with transmission electron microscopy, energy diffraction X-Ray analysis and 2-D gel electrophoresis to fulfill the objectives of the designed study. Data revealed that 500 µM Cd level altered physiology-based traits such as fresh and dry weights, tolerance index and chlorophyll-related parameters. It also caused lowering down of various essential plants nutrients as compared to control. The antioxidative metabolism was also significantly influenced by the higher level of Cd (500 µM). Oxidative stress biomarkers in all parts of the plant increased, while there was found either increase or decrease in the expression levels of various antioxidants. Ultramorphology of both roots and leaves was insignificantly changed. Cd was localized in cell wall, vacuoles and intracellular regions. Leaf proteomic data reveal differential expression of a number of proteins under Cd stressful conditions. Proteins such as methionine synthase, ribulose 1, 5-bisphosphate carboxylase, apoplastic anionic guiacol peroxidase etc. were upregulated. While proteins involved in energy pathway (ATP-dependent Clp protease ATP-binding subunit ClpC), chaperon and stress protein (HSP70 (AA6-651) etc. were supressed. Upregulation of antioxidant machinery, Cd-deposition in dead parts of the cell and active regulation of certain proteins show that ZMS-49 can be a potential candidate to be grown in Cd-contaminated areas.